Restriction map for
pFN21AA1047
(Circular) (Six-base) MAPSORT of: pFN21AA1047.seq Check: 7767 from: 1 to: 11716
pFN21AA1047 11716 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6750 7648 6750
Size: 898 10818
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 9526 278
Size: 53 83 186 936 7990 2468
Fragments arranged by size:
7990 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2084 6587 9059 1937
Size: 147 4503 2472 4594
Fragments arranged by size:
4594 4503 2472 147
Acc65I G'GTAC_C
Cuts at: 5453 9037 5453
Size: 3584 8132
AclI AA'CG_TT
Cuts at: 1563 9843 10216 1563
Size: 8280 373 3063
Fragments arranged by size:
8280 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3837 4398 4488 4826 5057 5130 5172 7314
Size: 2205 561 90 338 231 73 42 2142
Cuts at: 7314 8733 9779 11163 11431 1632
Size: 1419 1046 1384 268 1917
Fragments arranged by size:
2205 2142 1917 1419 1384 1046 561 338
268 231 90 73 42
AfeI AGC'GCT
Cuts at: 4705 4705
Size: 11716
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 11519
AflIII A'CryG_T
Cuts at: 1203 6396 10615 11592 1203
Size: 5193 4219 977 1327
Fragments arranged by size:
5193 4219 1327 977
AgeI A'CCGG_T
Cuts at: 10547 10547
Size: 11716
AhdI GACnn_n'nnGTC
Cuts at: 5751 10445 5751
Size: 4694 7022
AleI CACnn'nnGTG
Cuts at: 1166 6401 1166
Size: 5235 6481
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4668 4869 1184
Size: 3484 201 8031
Fragments arranged by size:
8031 3484 201
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2583 2615 7051 7083 2583
Size: 32 4436 32 7216
Fragments arranged by size:
7216 4436 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2126 2240 3869 4298 6659 6800 6976 7178 7541
Size: 114 1629 429 2361 141 176 202 363
Cuts at: 7541 8219 8393 8952 9459 11031 2126
Size: 678 174 559 507 1572 2811
Fragments arranged by size:
2811 2361 1629 1572 678 559 507 429
363 202 176 174 141 114
ApaI G_GGCC'C
Cuts at: 5431 7460 5431
Size: 2029 9687
ApaLI G'TGCA_C
Cuts at: 2393 6492 9773 10929 2393
Size: 4099 3281 1156 3180
Fragments arranged by size:
4099 3281 3180 1156
ApoI r'AATT_y
Cuts at: 1493 2225 2760 3048 5378 5508 9025 9251 9403
Size: 732 535 288 2330 130 3517 226 152
Cuts at: 9403 11512 1493
Size: 2109 1697
Fragments arranged by size:
3517 2330 2109 1697 732 535 288 226
152 130
AseI AT'TA_AT
Cuts at: 160 1032 10270 160
Size: 872 9238 1606
Fragments arranged by size:
9238 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11716
AvaI C'yCGr_G
Cuts at: 1870 1943 3711 4281 4664 5487 5736 9041 1870
Size: 73 1768 570 383 823 249 3305 4545
Fragments arranged by size:
4545 3305 1768 823 570 383 249 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2808 2841 2808
Size: 33 11683
BamHI G'GATC_C
Cuts at: 2440 6757 6787 7414 9046 9413 2440
Size: 4317 30 627 1632 367 4743
Fragments arranged by size:
4743 4317 1632 627 367 30
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4534 5453 5555 6041 9037
Size: 325 217 627 2747 919 102 486 2996
Cuts at: 9037 10492 618
Size: 1455 1842
Fragments arranged by size:
2996 2747 1842 1455 919 627 486 325
217 102
BanII G_rGCy'C
Cuts at: 1391 3165 3825 4317 4671 5431 5543 6470 7460
Size: 1774 660 492 354 760 112 927 990
Cuts at: 7460 8133 8142 9035 1391
Size: 673 9 893 4072
Fragments arranged by size:
4072 1774 990 927 893 760 673 660
492 354 112 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3636 4182 5637 8493 8817 928
Size: 971 1737 546 1455 2856 324 3827
Fragments arranged by size:
3827 2856 1737 1455 971 546 324
BbvCI CC'TCA_GC
Cuts at: 2414 2414
Size: 11716
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2180 2214 3503 3537 9907 9941 1903
Size: 34 243 34 1289 34 6370 34 3678
Fragments arranged by size:
6370 3678 1289 243 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4885 6647 9608 10818 4885
Size: 1762 2961 1210 5783
Fragments arranged by size:
5783 2961 1762 1210
BclI T'GATC_A
Cuts at: 1548 2506 2938 7839 7852 8673 1548
Size: 958 432 4901 13 821 4591
Fragments arranged by size:
4901 4591 958 821 432 13
BfrBI ATG'CAT
Cuts at: 4550 4954 4550
Size: 404 11312
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2410 3750 3809
Size: 107 122 71 631 605 738 1340 59
Cuts at: 3809 4735 7296 10327 136
Size: 926 2561 3031 1525
Fragments arranged by size:
3031 2561 1525 1340 926 738 631 605
122 107 71 59
BglII A'GATC_T
Cuts at: 3342 8397 11711 3342
Size: 5055 3314 3347
Fragments arranged by size:
5055 3347 3314
BlpI GC'TnA_GC
Cuts at: 4641 7425 8382 8873 9133 4641
Size: 2784 957 491 260 7224
Fragments arranged by size:
7224 2784 957 491 260
Bme1580I G_kGCm'C
Cuts at: 1790 2397 5431 5558 6209 6496 7460 7824 9777
Size: 607 3034 127 651 287 964 364 1953
Cuts at: 9777 10933 1790
Size: 1156 2573
Fragments arranged by size:
3034 2573 1953 1156 964 651 607 364
287 127
BmgBI CAC'GTC
Cuts at: 1318 2333 6399 8119 1318
Size: 1015 4066 1720 4915
Fragments arranged by size:
4915 4066 1720 1015
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2291 3952 4329
Size: 416 53 83 399 24 874 1661 377
Cuts at: 4329 4666 4904 6986 7362 9447 10405 442
Size: 337 238 2082 376 2085 958 1753
Fragments arranged by size:
2085 2082 1753 1661 958 874 416 399
377 376 337 238 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 11716
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5856 8161 5856
Size: 2305 9411
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4991 6138 6636 6819 7134 8040 10376 1239
Size: 3752 1147 498 183 315 906 2336 2579
Fragments arranged by size:
3752 2579 2336 1147 906 498 315 183
Bpu10I CC'TnA_GC
Cuts at: 2414 2655 4128 4351 2414
Size: 241 1473 223 9779
Fragments arranged by size:
9779 1473 241 223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2235 4331 5262 9841 10706 11004 2235
Size: 2096 931 4579 865 298 2947
Fragments arranged by size:
4579 2947 2096 931 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3869 4388 6371 6986 7340 7357 8056
Size: 617 2370 519 1983 615 354 17 699
Cuts at: 8056 8987 10379 882
Size: 931 1392 2219
Fragments arranged by size:
2370 2219 1983 1392 931 699 617 615
519 354 17
BsaAI yAC'GTr
Cuts at: 493 1204 4698 9507 493
Size: 711 3494 4809 2702
Fragments arranged by size:
4809 3494 2702 711
BsaBI GATnn'nnATC
Cuts at: 2730 4446 6699 11710 2730
Size: 1716 2253 5011 2736
Fragments arranged by size:
5011 2736 2253 1716
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 8792 9523 9905 10540
Size: 53 83 186 936 7259 731 382 635
Cuts at: 10540 275
Size: 1451
Fragments arranged by size:
7259 1451 936 731 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 8161 10149 10547 10821 10968 11379 8161
Size: 1988 398 274 147 411 8498
Fragments arranged by size:
8498 1988 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2251 2281 6966 6996 7051
Size: 30 165 30 848 30 4685 30 55
Cuts at: 7051 7081 7102 7132 7337 7367 1178
Size: 30 21 30 205 30 5527
Fragments arranged by size:
5527 4685 848 205 165 55 30 30
30 30 30 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2058 2670 4988 7059 8125 8338 8667 8746
Size: 872 612 2318 2071 1066 213 329 79
Cuts at: 8746 8922 1186
Size: 176 3980
Fragments arranged by size:
3980 2318 2071 1066 872 612 329 213
176 79
BseYI C'CCAG_C
Cuts at: 4250 6640 6991 7451 7914 8556 10919 11415 4250
Size: 2390 351 460 463 642 2363 496 4551
Fragments arranged by size:
4551 2390 2363 642 496 463 460 351
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4209 4314 4720 4864 7527 1163
Size: 573 2473 105 406 144 2663 5352
Fragments arranged by size:
5352 2663 2473 573 406 144 105
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6179 9166 9927 10955 1540
Size: 275 186 4178 2987 761 1028 2301
Fragments arranged by size:
4178 2987 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2397 4219 4461 6173 6496 8908 9777 9862 10933
Size: 1822 242 1712 323 2412 869 85 1071
Cuts at: 10933 2397
Size: 3180
Fragments arranged by size:
3180 2412 1822 1712 1071 869 323 242
85
BsmI GAATG_Cn'
Cuts at: 2039 2447 5523 5625 5737 6306 9227 9320 2039
Size: 408 3076 102 112 569 2921 93 4435
Fragments arranged by size:
4435 3076 2921 569 408 112 102 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4804 8125 4804
Size: 3321 8395
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2397 3165 3825 4219 4317 4461 4671
Size: 399 607 768 660 394 98 144 210
Cuts at: 4671 5431 5543 5558 6173 6209 6470 6496 7460
Size: 760 112 15 615 36 261 26 964
Cuts at: 7460 7824 8133 8142 8908 9035 9777 9862 10933
Size: 364 309 9 766 127 742 85 1071
Cuts at: 10933 1391
Size: 2174
Fragments arranged by size:
2174 1071 964 768 766 760 742 660
615 607 399 394 364 309 261 210
144 127 112 98 85 36 26 15
9
BspEI T'CCGG_A
Cuts at: 11379 11379
Size: 11716
BspHI T'CATG_A
Cuts at: 2305 5767 6106 6595 9605 11335 2305
Size: 3462 339 489 3010 1730 2686
Fragments arranged by size:
3462 3010 2686 1730 489 339
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4306 6750 7272 7648 9071 844
Size: 3462 2444 522 376 1423 3489
Fragments arranged by size:
3489 3462 2444 1423 522 376
BsrBI CCG'CTC
Cuts at: 1394 1823 4917 7210 7222 7228 8244 9603 1394
Size: 429 3094 2293 12 6 1016 1359 3507
Fragments arranged by size:
3507 3094 2293 1359 1016 429 12 6
BsrDI GCAATG_nn'
Cuts at: 65 1248 3120 4956 6873 10211 10385 65
Size: 1183 1872 1836 1917 3338 174 1396
Fragments arranged by size:
3338 1917 1872 1836 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 10360 10547 1700
Size: 110 8550 187 2869
Fragments arranged by size:
8550 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 8761 96
Size: 8665 3051
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11716
BssSI C'ACGA_G
Cuts at: 4074 9776 10788 4074
Size: 5702 1012 5002
Fragments arranged by size:
5702 5002 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2401 3953 6800 7178 7541 8219 794
Size: 1607 1552 2847 378 363 678 4291
Fragments arranged by size:
4291 2847 1607 1552 678 378 363
BstBI TT'CG_AA
Cuts at: 2200 3052 10520 2200
Size: 852 7468 3396
Fragments arranged by size:
7468 3396 852
BstEII G'GTnAC_C
Cuts at: 1184 6839 1184
Size: 5655 6061
BstXI CCAn_nnnn'nTGG
Cuts at: 6754 6754
Size: 11716
BstYI r'GATC_y
Cuts at: 1972 2440 2598 3342 4787 4828 5303 6757 6787
Size: 468 158 744 1445 41 475 1454 30
Cuts at: 6787 7414 8397 8949 9046 9413 9798 9815 11267
Size: 627 983 552 97 367 385 17 1452
Cuts at: 11267 11353 11365 11711 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1454 1452 1445 983 744 627 552
475 468 385 367 346 158 97 86
41 30 17 12
BstZ17I GTA'TAC
Cuts at: 2085 2085
Size: 11716
Bsu36I CC'TnA_GG
Cuts at: 5409 5409
Size: 11716
BtgI C'CryG_G
Cuts at: 513 1065 4320 5324 6295 7074 8304 513
Size: 552 3255 1004 971 779 1230 3925
Fragments arranged by size:
3925 3255 1230 1004 971 779 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 7620 487
Size: 683 56 6394 4583
Fragments arranged by size:
6394 4583 683 56
BtsI GCAGTG_nn'
Cuts at: 6437 6714 7442 7497 8972 9232 10026 10046 6437
Size: 277 728 55 1475 260 794 20 8107
Fragments arranged by size:
8107 1475 794 728 277 260 55 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 11716
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 11681
DraI TTT'AAA
Cuts at: 2884 5781 9020 2884
Size: 2897 3239 5580
Fragments arranged by size:
5580 3239 2897
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5926 7299 10723 809
Size: 507 4610 1373 3424 1802
Fragments arranged by size:
4610 3424 1802 1373 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 6176 6945 7268
Size: 54 1425 50 275 18 4346 769 323
Cuts at: 7268 7288 9163 10052 11681 8
Size: 20 1875 889 1629 43
Fragments arranged by size:
4346 1875 1629 1425 889 769 323 275
54 50 43 20 18
EagI C'GGCC_G
Cuts at: 1537 1812 6176 9163 1537
Size: 275 4364 2987 4090
Fragments arranged by size:
4364 4090 2987 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2535 2631 3404 3552 3603 3813 4074 4464
Size: 1182 96 773 148 51 210 261 390
Cuts at: 4464 8128 8614 8752 8827 9646 1353
Size: 3664 486 138 75 819 3423
Fragments arranged by size:
3664 3423 1182 819 773 486 390 261
210 148 138 96 75 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 10303 10677 10823 387
Size: 1064 138 8714 374 146 1280
Fragments arranged by size:
8714 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3837 4398 4488 4826 4991 5057 5130
Size: 393 2205 561 90 338 165 66 73
Cuts at: 5130 5172 6138 6636 6819 7134 7314 8040 8733
Size: 42 966 498 183 315 180 726 693
Cuts at: 8733 9779 10376 11163 11431 1239
Size: 1046 597 787 268 1524
Fragments arranged by size:
2205 1524 1046 966 787 726 693 597
561 498 393 338 315 268 183 180
165 90 73 66 42
EcoNI CCTnn'n_nnAGG
Cuts at: 5646 7050 8646 8718 5646
Size: 1404 1596 72 8644
Fragments arranged by size:
8644 1596 1404 72
EcoO109I rG'GnC_Cy
Cuts at: 3280 5427 6278 6501 7456 7457 7827 8632 8817
Size: 2147 851 223 955 1 370 805 185
Cuts at: 8817 3280
Size: 6179
Fragments arranged by size:
6179 2147 955 851 805 370 223 185
1
EcoRI G'AATT_C
Cuts at: 9025 9403 9025
Size: 378 11338
EcoRV GAT'ATC
Cuts at: 7578 7578
Size: 11716
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2226 3361 4128 4236 6002 2226
Size: 1135 767 108 1766 7940
Fragments arranged by size:
7940 1766 1135 767 108
FspI TGC'GCA
Cuts at: 10222 10222
Size: 11716
HaeII r_GCGC'y
Cuts at: 1678 4707 10863 1678
Size: 3029 6156 2531
Fragments arranged by size:
6156 3029 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2545 2577 3149 3181 4658
Size: 32 135 32 973 32 572 32 1477
Cuts at: 4658 4690 4779 4811 5086 5118 5205 5237 5737
Size: 32 89 32 275 32 87 32 500
Cuts at: 5737 5769 6215 6247 6670 6702 6873 6905 7567
Size: 32 446 32 423 32 171 32 662
Cuts at: 7567 7599 8228 8260 8389 8421 8804 8836 8984
Size: 32 629 32 129 32 383 32 148
Cuts at: 8984 9016 10357 10389 10431 10463 1373
Size: 32 1341 32 42 32 2626
Fragments arranged by size:
2626 1477 1341 973 662 629 572 500
446 423 383 275 171 148 135 129
89 87 42 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 9060 9306 669
Size: 988 281 7122 246 3079
Fragments arranged by size:
7122 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 5971 8285 8515 748
Size: 5223 2314 230 3949
Fragments arranged by size:
5223 3949 2314 230
HpaI GTT'AAC
Cuts at: 9306 9306
Size: 11716
KpnI G_GTAC'C
Cuts at: 5457 9041 5457
Size: 3584 8132
MfeI C'AATT_G
Cuts at: 3951 4899 9315 3951
Size: 948 4416 6352
Fragments arranged by size:
6352 4416 948
MluI A'CGCG_T
Cuts at: 11592 11592
Size: 11716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5150 5522 6125 7055 7278 7442 8982 10830
Size: 3845 372 603 930 223 164 1540 1848
Cuts at: 10830 11014 1305
Size: 184 2007
Fragments arranged by size:
3845 2007 1848 1540 930 603 372 223
184 164
MscI TGG'CCA
Cuts at: 10 64 1489 1832 6947 10
Size: 54 1425 343 5115 4779
Fragments arranged by size:
5115 4779 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3260 5439 6335 6401 7308 7590 9674
Size: 648 2094 2179 896 66 907 282 2084
Cuts at: 9674 10033 10192 518
Size: 359 159 2042
Fragments arranged by size:
2179 2094 2084 2042 907 896 648 359
282 159 66
MspA1I CmG'CkG
Cuts at: 1606 2401 3788 3809 4376 4981 6310 6797 7175
Size: 795 1387 21 567 605 1329 487 378
Cuts at: 7175 7783 8378 9132 9809 10957 11202 1606
Size: 608 595 754 677 1148 245 2120
Fragments arranged by size:
2120 1387 1329 1148 795 754 677 608
605 595 567 487 378 245 21
NcoI C'CATG_G
Cuts at: 513 1065 7074 8304 513
Size: 552 6009 1230 3925
Fragments arranged by size:
6009 3925 1230 552
NdeI CA'TA_TG
Cuts at: 387 5716 6064 387
Size: 5329 348 6039
Fragments arranged by size:
6039 5329 348
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 11716
NotI GC'GGCC_GC
Cuts at: 9163 9163
Size: 11716
NsiI A_TGCA'T
Cuts at: 4552 4956 4552
Size: 404 11312
NspI r_CATG'y
Cuts at: 1132 1229 8904 9074 10619 1132
Size: 97 7675 170 1545 2229
Fragments arranged by size:
7675 2229 1545 170 97
PasI CC'CwG_GG
Cuts at: 1348 5886 6202 6470 1348
Size: 4538 316 268 6594
Fragments arranged by size:
6594 4538 316 268
PciI A'CATG_T
Cuts at: 10615 10615
Size: 11716
PflMI CCAn_nnn'nTGG
Cuts at: 1108 6068 6659 8563 1108
Size: 4960 591 1904 4261
Fragments arranged by size:
4960 4261 1904 591
PfoI T'CCnGG_A
Cuts at: 4968 7150 4968
Size: 2182 9534
PmeI GTTT'AAAC
Cuts at: 9020 9020
Size: 11716
PmlI CAC'GTG
Cuts at: 4698 4698
Size: 11716
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7051 7083 9785 9817 11298 11330 7051
Size: 32 2702 32 1481 32 7437
Fragments arranged by size:
7437 2702 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3280 6501 7827 8632 3280
Size: 3221 1326 805 6364
Fragments arranged by size:
6364 3221 1326 805
PshAI GACnn'nnGTC
Cuts at: 1613 5637 1613
Size: 4024 7692
PsiI TTA'TAA
Cuts at: 3465 5207 8850 9286 3465
Size: 1742 3643 436 5895
Fragments arranged by size:
5895 3643 1742 436
PspOMI G'GGCC_C
Cuts at: 5427 7456 5427
Size: 2029 9687
PstI C_TGCA'G
Cuts at: 830 2428 3787 3802 6721 6796 7246 7537 7665
Size: 1598 1359 15 2919 75 450 291 128
Cuts at: 7665 8224 9068 830
Size: 559 844 3478
Fragments arranged by size:
3478 2919 1598 1359 844 559 450 291
128 75 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11716
PvuII CAG'CTG
Cuts at: 3788 4376 7175 7783 8378 3788
Size: 588 2799 608 595 7126
Fragments arranged by size:
7126 2799 608 595 588
SalI G'TCGA_C
Cuts at: 1936 9058 1936
Size: 7122 4594
SanDI GG'GwC_CC
Cuts at: 8632 8632
Size: 11716
SapI GCTCTTCn'nnn_
Cuts at: 3813 4074 3813
Size: 261 11455
SbfI CC_TGCA'GG
Cuts at: 9068 9068
Size: 11716
ScaI AGT'ACT
Cuts at: 8881 9964 11676 8881
Size: 1083 1712 8921
Fragments arranged by size:
8921 1712 1083
SexAI A'CCwGG_T
Cuts at: 2850 3761 8038 2850
Size: 911 4277 6528
Fragments arranged by size:
6528 4277 911
SfcI C'TryA_G
Cuts at: 826 1045 2424 3783 3798 3861 6717 6792 7242
Size: 219 1379 1359 15 63 2856 75 450
Cuts at: 7242 7533 7661 8220 9064 10199 10880 11071 826
Size: 291 128 559 844 1135 681 191 1471
Fragments arranged by size:
2856 1471 1379 1359 1135 844 681 559
450 291 219 191 128 75 63 15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11716
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11716
SmaI CCC'GGG
Cuts at: 1872 3713 9043 1872
Size: 1841 5330 4545
Fragments arranged by size:
5330 4545 1841
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2250 4281 4310 5277 9820 10721
Size: 197 926 307 2031 29 967 4543 901
Cuts at: 10721 10983 820
Size: 262 1553
Fragments arranged by size:
4543 2031 1553 967 926 901 307 262
197 29
SnaBI TAC'GTA
Cuts at: 493 9507 493
Size: 9014 2702
SpeI A'CTAG_T
Cuts at: 152 2264 152
Size: 2112 9604
SphI G_CATG'C
Cuts at: 1132 9074 1132
Size: 7942 3774
SspI AAT'ATT
Cuts at: 5 52 5725 9640 5
Size: 47 5673 3915 2081
Fragments arranged by size:
5673 3915 2081 47
StuI AGG'CCT
Cuts at: 1517 2248 7476 8092 1517
Size: 731 5228 616 5141
Fragments arranged by size:
5228 5141 731 616
StyI C'CwwG_G
Cuts at: 513 1065 2032 2820 3648 6116 7074 7378 7471
Size: 552 967 788 828 2468 958 304 93
Cuts at: 7471 8304 9155 513
Size: 833 851 3074
Fragments arranged by size:
3074 2468 967 958 851 833 828 788
552 304 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2247 4348 5302 6556 7173 8317 9743
Size: 1255 667 2101 954 1254 617 1144 1426
Cuts at: 9743 9760 9913 10098 10543 325
Size: 17 153 185 445 1498
Fragments arranged by size:
2101 1498 1426 1255 1254 1144 954 667
617 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4337 4364 8761
Size: 274 80 33 51 1443 2360 27 4397
Cuts at: 8761 8879 9962 11674 96
Size: 118 1083 1712 138
Fragments arranged by size:
4397 2360 1712 1443 1083 274 138 118
80 51 33 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4896 4928 6966 6998 7078 7110 11676 11708 4896
Size: 32 2038 32 80 32 4566 32 4904
Fragments arranged by size:
4904 4566 2038 80 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2331 6671 8998 1316
Size: 1015 4340 2327 4034
Fragments arranged by size:
4340 4034 2327 1015
XbaI T'CTAG_A
Cuts at: 8441 9052 8441
Size: 611 11105
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4606 5058 5179 5296 6088 7385 8802 4606
Size: 452 121 117 792 1297 1417 7520
Fragments arranged by size:
7520 1417 1297 792 452 121 117
XhoI C'TCGA_G
Cuts at: 1943 4281 1943
Size: 2338 9378
XmaI C'CCGG_G
Cuts at: 1870 3711 9041 1870
Size: 1841 5330 4545
Fragments arranged by size:
5330 4545 1841
XmnI GAAnn'nnTTC
Cuts at: 2277 3078 5070 5115 9845 2277
Size: 801 1992 45 4730 4148
Fragments arranged by size:
4730 4148 1992 801 45
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 9524 276
Size: 53 83 186 936 7990 2468
Fragments arranged by size:
7990 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BbeI BsiWI DraIII EcoICRI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PspXI PsrI
RsrII SacI SacII SfiI SfoI SrfI SwaI