Restriction map for
pFN21AA1040
(Circular) (Six-base) MAPSORT of: pFN21AA1040.seq Check: 47 from: 1 to: 9859
pFN21AA1040 9859 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7669 278
Size: 53 83 186 936 6133 2468
Fragments arranged by size:
6133 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3584 7202 1937
Size: 1647 3618 4594
Fragments arranged by size:
4594 3618 1647
Acc65I G'GTAC_C
Cuts at: 7180 7180
Size: 9859
AclI AA'CG_TT
Cuts at: 1563 7986 8359 1563
Size: 6423 373 3063
Fragments arranged by size:
6423 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2571 3465 3579 4103 4778 6763 7053 7922
Size: 939 894 114 524 675 1985 290 869
Cuts at: 7922 9306 9574 1632
Size: 1384 268 1917
Fragments arranged by size:
1985 1917 1384 939 894 869 675 524
290 268 114
AflII C'TTAA_G
Cuts at: 820 1017 2369 6974 820
Size: 197 1352 4605 3705
Fragments arranged by size:
4605 3705 1352 197
AflIII A'CryG_T
Cuts at: 1203 8758 9735 1203
Size: 7555 977 1327
Fragments arranged by size:
7555 1327 977
AgeI A'CCGG_T
Cuts at: 8690 8690
Size: 9859
AhdI GACnn_n'nnGTC
Cuts at: 8588 8588
Size: 9859
AleI CACnn'nnGTG
Cuts at: 1166 2083 1166
Size: 917 8942
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2957 1184
Size: 1773 8086
AlwNI CAG_nnn'CTG
Cuts at: 2501 3090 4588 4681 7602 9174 2501
Size: 589 1498 93 2921 1572 3186
Fragments arranged by size:
3186 2921 1572 1498 589 93
ApaI G_GGCC'C
Cuts at: 5610 5610
Size: 9859
ApaLI G'TGCA_C
Cuts at: 7916 9072 7916
Size: 1156 8703
ApoI r'AATT_y
Cuts at: 1493 2096 2169 2801 3026 4434 5520 6113 6461
Size: 603 73 632 225 1408 1086 593 348
Cuts at: 6461 6638 6737 7168 7394 7546 9655 1493
Size: 177 99 431 226 152 2109 1697
Fragments arranged by size:
2109 1697 1408 1086 632 603 593 431
348 226 225 177 152 99 73
AseI AT'TA_AT
Cuts at: 160 1032 4632 8413 160
Size: 872 3600 3781 1606
Fragments arranged by size:
3781 3600 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9859
AvaI C'yCGr_G
Cuts at: 1870 1943 2024 7184 1870
Size: 73 81 5160 4545
Fragments arranged by size:
5160 4545 81 73
BamHI G'GATC_C
Cuts at: 5110 7189 7556 5110
Size: 2079 367 7413
Fragments arranged by size:
7413 2079 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2012 4163 7180 8635 618
Size: 325 217 627 225 2151 3017 1455 1842
Fragments arranged by size:
3017 2151 1842 1455 627 325 225 217
BanII G_rGCy'C
Cuts at: 1391 5610 6677 7178 1391
Size: 4219 1067 501 4072
Fragments arranged by size:
4219 4072 1067 501
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3677 3790 4784 928
Size: 971 1778 113 994 6003
Fragments arranged by size:
6003 1778 994 971 113
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8050 8084 1903
Size: 34 6113 34 3678
Fragments arranged by size:
6113 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3351 6087 6406 7751 8961 3351
Size: 2736 319 1345 1210 4249
Fragments arranged by size:
4249 2736 1345 1210 319
BclI T'GATC_A
Cuts at: 1548 4753 1548
Size: 3205 6654
BfrBI ATG'CAT
Cuts at: 2651 5951 7053 2651
Size: 3300 1102 5457
Fragments arranged by size:
5457 3300 1102
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4714 8470 136
Size: 107 122 71 631 605 3042 3756 1525
Fragments arranged by size:
3756 3042 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2615 9854 2615
Size: 7239 2620
BlpI GC'TnA_GC
Cuts at: 7276 7276
Size: 9859
Bme1580I G_kGCm'C
Cuts at: 1790 5610 7018 7920 9076 1790
Size: 3820 1408 902 1156 2573
Fragments arranged by size:
3820 2573 1408 1156 902
BmgBI CAC'GTC
Cuts at: 1318 5349 1318
Size: 4031 5828
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3191 3797 4191
Size: 416 53 83 399 24 1774 606 394
Cuts at: 4191 7590 8548 442
Size: 3399 958 1753
Fragments arranged by size:
3399 1774 1753 958 606 416 399 394
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9859
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2212 2212
Size: 9859
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2066 2102 2883 5307 5388 6299 6513 6752
Size: 827 36 781 2424 81 911 214 239
Cuts at: 6752 8519 1239
Size: 1767 2579
Fragments arranged by size:
2579 2424 1767 911 827 781 239 214
81 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2774 3987 4487 4637 6786 7984 8849 9147 2774
Size: 1213 500 150 2149 1198 865 298 3486
Fragments arranged by size:
3486 2149 1213 1198 865 500 298 150
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4438 8522 882
Size: 617 2939 4084 2219
Fragments arranged by size:
4084 2939 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 7650 493
Size: 711 6446 2702
Fragments arranged by size:
6446 2702 711
BsaBI GATnn'nnATC
Cuts at: 5718 9853 5718
Size: 4135 5724
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7666 8048 8683 275
Size: 53 83 186 936 6133 382 635 1451
Fragments arranged by size:
6133 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 8292 8690 8964 9111 9522 8292
Size: 398 274 147 411 8629
Fragments arranged by size:
8629 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3687 3717 5761 5791 7143
Size: 30 165 30 2284 30 2044 30 1352
Cuts at: 7143 7173 1178
Size: 30 3864
Fragments arranged by size:
3864 2284 2044 1352 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2804 6395 1186
Size: 1618 3591 4650
Fragments arranged by size:
4650 3591 1618
BseYI C'CCAG_C
Cuts at: 9062 9558 9062
Size: 496 9363
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2139 3116 3678 5034 5157 7068 1163
Size: 573 403 977 562 1356 123 1911 3954
Fragments arranged by size:
3954 1911 1356 977 573 562 403 123
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7309 8070 9098 1540
Size: 275 186 5308 761 1028 2301
Fragments arranged by size:
5308 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 6315 7920 8005 9076 6315
Size: 1605 85 1071 7098
Fragments arranged by size:
7098 1605 1071 85
BsmI GAATG_Cn'
Cuts at: 3145 3467 3673 4670 4781 5188 7055 7370 7463
Size: 322 206 997 111 407 1867 315 93
Cuts at: 7463 3145
Size: 5541
Fragments arranged by size:
5541 1867 997 407 322 315 206 111
93
BsmBI CGTCTCn'nnnn_
Cuts at: 5339 5339
Size: 9859
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 5610 6315 6677 7018 7178 7920 8005
Size: 399 3820 705 362 341 160 742 85
Cuts at: 8005 9076 1391
Size: 1071 2174
Fragments arranged by size:
3820 2174 1071 742 705 399 362 341
160 85
BspEI T'CCGG_A
Cuts at: 9522 9522
Size: 9859
BspHI T'CATG_A
Cuts at: 2419 7748 9478 2419
Size: 5329 1730 2800
Fragments arranged by size:
5329 2800 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2770 4853 7214 844
Size: 1926 2083 2361 3489
Fragments arranged by size:
3489 2361 2083 1926
BsrBI CCG'CTC
Cuts at: 1394 1823 7746 1394
Size: 429 5923 3507
Fragments arranged by size:
5923 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4066 4347 5342 6159 6607 8354 8528
Size: 1183 2818 281 995 817 448 1747 174
Cuts at: 8528 65
Size: 1396
Fragments arranged by size:
2818 1747 1396 1183 995 817 448 281
174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6199 8503 8690 1700
Size: 110 4389 2304 187 2869
Fragments arranged by size:
4389 2869 2304 187 110
BsrGI T'GTAC_A
Cuts at: 96 2206 96
Size: 2110 7749
BssHII G'CGCG_C
Cuts at: 1925 2068 1925
Size: 143 9716
BssSI C'ACGA_G
Cuts at: 7919 8931 7919
Size: 1012 8847
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4681 794
Size: 3887 5972
BstBI TT'CG_AA
Cuts at: 8663 8663
Size: 9859
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9859
BstXI CCAn_nnnn'nTGG
Cuts at: 3463 3463
Size: 9859
BstYI r'GATC_y
Cuts at: 1972 2615 2668 3185 5110 5379 5759 6183 6848
Size: 643 53 517 1925 269 380 424 665
Cuts at: 6848 7189 7556 7941 7958 9410 9496 9508 9854
Size: 341 367 385 17 1452 86 12 346
Cuts at: 9854 1972
Size: 1977
Fragments arranged by size:
1977 1925 1452 665 643 517 424 385
380 367 346 341 269 86 53 17
12
Bsu36I CC'TnA_GG
Cuts at: 6633 6633
Size: 9859
BtgI C'CryG_G
Cuts at: 513 1065 4072 513
Size: 552 3007 6300
Fragments arranged by size:
6300 3007 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 6851 487
Size: 683 56 5625 3495
Fragments arranged by size:
5625 3495 683 56
BtsI GCAGTG_nn'
Cuts at: 3394 3879 3981 4224 4295 5259 6300 7375 8169
Size: 485 102 243 71 964 1041 1075 794
Cuts at: 8169 8189 3394
Size: 20 5064
Fragments arranged by size:
5064 1075 1041 964 794 485 243 102
71 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9859
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9824
DraI TTT'AAA
Cuts at: 3750 5031 6843 6945 7163 3750
Size: 1281 1812 102 218 6446
Fragments arranged by size:
6446 1812 1281 218 102
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8866 809
Size: 507 7550 1802
Fragments arranged by size:
7550 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4456 4715 5036
Size: 54 1425 50 275 18 2626 259 321
Cuts at: 5036 6476 7306 8195 9824 8
Size: 1440 830 889 1629 43
Fragments arranged by size:
2626 1629 1440 1425 889 830 321 275
259 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7306 1537
Size: 275 5494 4090
Fragments arranged by size:
5494 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4122 4980 5007 5126 6912 7789 1353
Size: 2769 858 27 119 1786 877 3423
Fragments arranged by size:
3423 2769 1786 877 858 119 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6218 8446 8820 8966 387
Size: 1064 138 4629 2228 374 146 1280
Fragments arranged by size:
4629 2228 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2066 2102 2571 2883 3465 3579 4103
Size: 393 434 36 469 312 582 114 524
Cuts at: 4103 4778 5307 5388 6299 6513 6752 6763 7053
Size: 675 529 81 911 214 239 11 290
Cuts at: 7053 7922 8519 9306 9574 1239
Size: 869 597 787 268 1524
Fragments arranged by size:
1524 911 869 787 675 597 582 529
524 469 434 393 312 290 268 239
214 114 81 36 11
EcoNI CCTnn'n_nnAGG
Cuts at: 6804 6804
Size: 9859
EcoO109I rG'GnC_Cy
Cuts at: 4193 5606 4193
Size: 1413 8446
EcoRI G'AATT_C
Cuts at: 7168 7546 7168
Size: 378 9481
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5239 6029 5239
Size: 790 9069
FspI TGC'GCA
Cuts at: 5169 8365 5169
Size: 3196 6663
FspAI rTGC'GCAy
Cuts at: 5169 5169
Size: 9859
HaeII r_GCGC'y
Cuts at: 1678 9006 1678
Size: 7328 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3590 3622 3925 3957 4277
Size: 32 135 32 2018 32 303 32 320
Cuts at: 4277 4309 4444 4476 6061 6093 6524 6556 8500
Size: 32 135 32 1585 32 431 32 1944
Cuts at: 8500 8532 8574 8606 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2018 1944 1585 431 320 303 135
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3585 3609 3983 7203 7449 669
Size: 988 281 1647 24 374 3220 246 3079
Fragments arranged by size:
3220 3079 1647 988 374 281 246 24
HindIII A'AGCT_T
Cuts at: 748 4518 5509 748
Size: 3770 991 5098
Fragments arranged by size:
5098 3770 991
HpaI GTT'AAC
Cuts at: 7449 7449
Size: 9859
KpnI G_GTAC'C
Cuts at: 7184 7184
Size: 9859
MfeI C'AATT_G
Cuts at: 2148 5160 7458 2148
Size: 3012 2298 4549
Fragments arranged by size:
4549 3012 2298
MluI A'CGCG_T
Cuts at: 9735 9735
Size: 9859
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3396 3458 3921 6211 8973 9157 1305
Size: 2091 62 463 2290 2762 184 2007
Fragments arranged by size:
2762 2290 2091 2007 463 184 62
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4458 4717 6478 10
Size: 54 1425 343 2626 259 1761 3391
Fragments arranged by size:
3391 2626 1761 1425 343 259 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2083 3461 3506 4071 4786 7817 8176
Size: 648 917 1378 45 565 715 3031 359
Cuts at: 8176 8335 518
Size: 159 2042
Fragments arranged by size:
3031 2042 1378 917 715 648 565 359
159 45
MspA1I CmG'CkG
Cuts at: 1606 2315 2498 6145 7275 7952 9100 9345 1606
Size: 709 183 3647 1130 677 1148 245 2120
Fragments arranged by size:
3647 2120 1148 1130 709 677 245 183
NcoI C'CATG_G
Cuts at: 513 1065 4072 513
Size: 552 3007 6300
Fragments arranged by size:
6300 3007 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9859
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9859
NotI GC'GGCC_GC
Cuts at: 7306 7306
Size: 9859
NsiI A_TGCA'T
Cuts at: 2653 5953 7055 2653
Size: 3300 1102 5457
Fragments arranged by size:
5457 3300 1102
NspI r_CATG'y
Cuts at: 1132 1229 2335 2938 6067 6449 7217 8762 1132
Size: 97 1106 603 3129 382 768 1545 2229
Fragments arranged by size:
3129 2229 1545 1106 768 603 382 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9859
PciI A'CATG_T
Cuts at: 8758 8758
Size: 9859
PflMI CCAn_nnn'nTGG
Cuts at: 1108 6100 6137 1108
Size: 4992 37 4830
Fragments arranged by size:
4992 4830 37
PfoI T'CCnGG_A
Cuts at: 2664 5514 2664
Size: 2850 7009
PmeI GTTT'AAAC
Cuts at: 7163 7163
Size: 9859
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7928 7960 9441 9473 7928
Size: 32 1481 32 8314
Fragments arranged by size:
8314 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4193 4193
Size: 9859
PshAI GACnn'nnGTC
Cuts at: 1613 2284 1613
Size: 671 9188
PsiI TTA'TAA
Cuts at: 3818 7429 3818
Size: 3611 6248
PspOMI G'GGCC_C
Cuts at: 5606 5606
Size: 9859
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2358 2390 2571 2603 2943 2975 2358
Size: 32 181 32 340 32 9242
Fragments arranged by size:
9242 340 181 32 32 32
PstI C_TGCA'G
Cuts at: 830 2314 3886 4593 4686 5266 5409 7211 830
Size: 1484 1572 707 93 580 143 1802 3478
Fragments arranged by size:
3478 1802 1572 1484 707 580 143 93
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9859
PvuII CAG'CTG
Cuts at: 2315 2498 6145 2315
Size: 183 3647 6029
Fragments arranged by size:
6029 3647 183
SalI G'TCGA_C
Cuts at: 1936 3583 7201 1936
Size: 1647 3618 4594
Fragments arranged by size:
4594 3618 1647
SbfI CC_TGCA'GG
Cuts at: 7211 7211
Size: 9859
ScaI AGT'ACT
Cuts at: 2589 5770 6958 8107 9819 2589
Size: 3181 1188 1149 1712 2629
Fragments arranged by size:
3181 2629 1712 1188 1149
SexAI A'CCwGG_T
Cuts at: 5744 5744
Size: 9859
SfcI C'TryA_G
Cuts at: 826 1045 2310 2505 3882 4185 4589 4682 5262
Size: 219 1265 195 1377 303 404 93 580
Cuts at: 5262 5405 5574 7207 8342 9023 9214 826
Size: 143 169 1633 1135 681 191 1471
Fragments arranged by size:
1633 1471 1377 1265 1135 681 580 404
303 219 195 191 169 143 93
SfiI GGCCn_nnn'nGGCC
Cuts at: 4714 4714
Size: 9859
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9859
SgrAI Cr'CCGG_yG
Cuts at: 1700 6199 1700
Size: 4499 5360
SmaI CCC'GGG
Cuts at: 1872 7186 1872
Size: 5314 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2369 2753 4002 4466 4652 6801
Size: 197 926 426 384 1249 464 186 2149
Cuts at: 6801 6974 7963 8864 9126 820
Size: 173 989 901 262 1553
Fragments arranged by size:
2149 1553 1249 989 926 901 464 426
384 262 197 186 173
SnaBI TAC'GTA
Cuts at: 493 7650 493
Size: 7157 2702
SpeI A'CTAG_T
Cuts at: 152 7099 152
Size: 6947 2912
SphI G_CATG'C
Cuts at: 1132 2938 6449 7217 1132
Size: 1806 3511 768 3774
Fragments arranged by size:
3774 3511 1806 768
SspI AAT'ATT
Cuts at: 5 52 2482 3745 4630 4897 4930 5299 6322
Size: 47 2430 1263 885 267 33 369 1023
Cuts at: 6322 6571 7783 5
Size: 249 1212 2081
Fragments arranged by size:
2430 2081 1263 1212 1023 885 369 267
249 47 33
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9859
StyI C'CwwG_G
Cuts at: 513 1065 2235 3153 4072 6911 7298 513
Size: 552 1170 918 919 2839 387 3074
Fragments arranged by size:
3074 2839 1170 919 918 552 387
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5084 5704 7886 7903 8056 8241 8686
Size: 1255 3504 620 2182 17 153 185 445
Cuts at: 8686 325
Size: 1498
Fragments arranged by size:
3504 2182 1498 1255 620 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2206 2587 4199
Size: 274 80 33 51 1443 229 381 1612
Cuts at: 4199 4832 5768 6010 6859 6956 8105 9817 96
Size: 633 936 242 849 97 1149 1712 138
Fragments arranged by size:
1712 1612 1443 1149 936 849 633 381
274 242 229 138 97 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3687 3719 9819 9851 3687
Size: 32 6100 32 3695
Fragments arranged by size:
6100 3695 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9859
XbaI T'CTAG_A
Cuts at: 4333 7195 4333
Size: 2862 6997
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9859
XmaI C'CCGG_G
Cuts at: 1870 7184 1870
Size: 5314 4545
XmnI GAAnn'nnTTC
Cuts at: 2096 5510 5644 6504 7988 2096
Size: 3414 134 860 1484 3967
Fragments arranged by size:
3967 3414 1484 860 134
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7667 276
Size: 53 83 186 936 6133 2468
Fragments arranged by size:
6133 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoRV FseI KasI
NaeI NarI NgoMIV NruI PacI PmlI PspXI RsrII
SacI SacII SanDI SapI SfoI SrfI SwaI XcmI