Restriction map for
pFN21AA1035
(Circular) (Six-base) MAPSORT of: pFN21AA1035.seq Check: 5338 from: 1 to: 8383
pFN21AA1035 8383 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4448 4448
Size: 8383
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6193 278
Size: 53 83 186 936 4657 2468
Fragments arranged by size:
4657 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2727 3479 5726 1937
Size: 790 752 2247 4594
Fragments arranged by size:
4594 2247 790 752
Acc65I G'GTAC_C
Cuts at: 5360 5704 5360
Size: 344 8039
AclI AA'CG_TT
Cuts at: 1563 4802 6510 6883 1563
Size: 3239 1708 373 3063
Fragments arranged by size:
3239 3063 1708 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2085 2528 3108 3822 6446 7830 8098 1632
Size: 453 443 580 714 2624 1384 268 1917
Fragments arranged by size:
2624 1917 1384 714 580 453 443 268
AflII C'TTAA_G
Cuts at: 820 1017 2263 820
Size: 197 1246 6940
Fragments arranged by size:
6940 1246 197
AflIII A'CryG_T
Cuts at: 1203 5260 7282 8259 1203
Size: 4057 2022 977 1327
Fragments arranged by size:
4057 2022 1327 977
AgeI A'CCGG_T
Cuts at: 7214 7214
Size: 8383
AhdI GACnn_n'nnGTC
Cuts at: 7112 7112
Size: 8383
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8383
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8383
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3264 3296 3747 3779 5529 5561 3264
Size: 32 451 32 1750 32 6086
Fragments arranged by size:
6086 1750 451 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2114 4454 6126 7698 2114
Size: 2340 1672 1572 2799
Fragments arranged by size:
2799 2340 1672 1572
ApaLI G'TGCA_C
Cuts at: 6440 7596 6440
Size: 1156 7227
ApoI r'AATT_y
Cuts at: 1493 2136 2208 2447 2494 2509 2578 2650 2817
Size: 643 72 239 47 15 69 72 167
Cuts at: 2817 2892 3181 3406 3826 4136 4148 5390 5692
Size: 75 289 225 420 310 12 1242 302
Cuts at: 5692 5918 6070 8179 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1242 643 420 310 302 289
239 226 225 167 152 75 72 72
69 47 15 12
AseI AT'TA_AT
Cuts at: 160 1032 2469 6937 160
Size: 872 1437 4468 1606
Fragments arranged by size:
4468 1606 1437 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8383
AvaI C'yCGr_G
Cuts at: 1870 1943 4755 5364 5708 1870
Size: 73 2812 609 344 4545
Fragments arranged by size:
4545 2812 609 344 73
BamHI G'GATC_C
Cuts at: 2938 5713 6080 2938
Size: 2775 367 5241
Fragments arranged by size:
5241 2775 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2772 5360 5704 7159 618
Size: 325 217 627 985 2588 344 1455 1842
Fragments arranged by size:
2588 1842 1455 985 627 344 325 217
BanII G_rGCy'C
Cuts at: 1391 2479 5702 1391
Size: 1088 3223 4072
Fragments arranged by size:
4072 3223 1088
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3674 928
Size: 971 1775 5637
Fragments arranged by size:
5637 1775 971
BbvCI CC'TCA_GC
Cuts at: 4608 4608
Size: 8383
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2504 2538 6574 6608 1903
Size: 34 567 34 4036 34 3678
Fragments arranged by size:
4036 3678 567 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2596 5149 5156 6275 7485 2596
Size: 2553 7 1119 1210 3494
Fragments arranged by size:
3494 2553 1210 1119 7
BclI T'GATC_A
Cuts at: 1548 3547 5329 1548
Size: 1999 1782 4602
Fragments arranged by size:
4602 1999 1782
BfrBI ATG'CAT
Cuts at: 4576 4576
Size: 8383
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6994 136
Size: 107 122 71 631 605 5322 1525
Fragments arranged by size:
5322 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2239 3256 8378 2239
Size: 1017 5122 2244
Fragments arranged by size:
5122 2244 1017
BlpI GC'TnA_GC
Cuts at: 2094 3066 5800 2094
Size: 972 2734 4677
Fragments arranged by size:
4677 2734 972
Bme1580I G_kGCm'C
Cuts at: 1790 6444 7600 1790
Size: 4654 1156 2573
Fragments arranged by size:
4654 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8383
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6114 7072 442
Size: 416 53 83 399 24 4697 958 1753
Fragments arranged by size:
4697 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8383
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2099 2941 3810 4788 7043 1239
Size: 860 842 869 978 2255 2579
Fragments arranged by size:
2579 2255 978 869 860 842
Bpu10I CC'TnA_GC
Cuts at: 3285 4608 4992 3285
Size: 1323 384 6676
Fragments arranged by size:
6676 1323 384
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4017 4423 5619 6508 7373 7671 4017
Size: 406 1196 889 865 298 4729
Fragments arranged by size:
4729 1196 889 865 406 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7046 882
Size: 617 5547 2219
Fragments arranged by size:
5547 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3362 4557 5261 6174 493
Size: 711 2158 1195 704 913 2702
Fragments arranged by size:
2702 2158 1195 913 711 704
BsaBI GATnn'nnATC
Cuts at: 4971 8377 4971
Size: 3406 4977
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6190 6572 7207 275
Size: 53 83 186 936 4657 382 635 1451
Fragments arranged by size:
4657 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4963 6816 7214 7488 7635 8046 4963
Size: 1853 398 274 147 411 5300
Fragments arranged by size:
5300 1853 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2985 3015 3858 3888 1178
Size: 30 165 30 1582 30 843 30 5673
Fragments arranged by size:
5673 1582 843 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 8383
BseYI C'CCAG_C
Cuts at: 3228 5200 7586 8082 3228
Size: 1972 2386 496 3529
Fragments arranged by size:
3529 2386 1972 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3769 5589 1163
Size: 573 2033 1820 3957
Fragments arranged by size:
3957 2033 1820 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5833 6594 7622 1540
Size: 275 186 3832 761 1028 2301
Fragments arranged by size:
3832 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 6444 6529 7600 6444
Size: 85 1071 7227
Fragments arranged by size:
7227 1071 85
BsmI GAATG_Cn'
Cuts at: 2268 2368 4275 5894 5987 2268
Size: 100 1907 1619 93 4664
Fragments arranged by size:
4664 1907 1619 100 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2479 5702 6444 6529 7600 1391
Size: 399 689 3223 742 85 1071 2174
Fragments arranged by size:
3223 2174 1071 742 689 399 85
BspEI T'CCGG_A
Cuts at: 4963 8046 4963
Size: 3083 5300
BspHI T'CATG_A
Cuts at: 4815 6272 8002 4815
Size: 1457 1730 5196
Fragments arranged by size:
5196 1730 1457
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4448 5738 844
Size: 3604 1290 3489
Fragments arranged by size:
3604 3489 1290
BsrBI CCG'CTC
Cuts at: 1394 1823 6270 1394
Size: 429 4447 3507
Fragments arranged by size:
4447 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2690 3617 4692 5171 6878 7052 65
Size: 1183 1442 927 1075 479 1707 174 1396
Fragments arranged by size:
1707 1442 1396 1183 1075 927 479 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2787 7027 7214 1700
Size: 110 977 4240 187 2869
Fragments arranged by size:
4240 2869 977 187 110
BsrGI T'GTAC_A
Cuts at: 96 4012 96
Size: 3916 4467
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8383
BssSI C'ACGA_G
Cuts at: 6443 7455 6443
Size: 1012 7371
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2512 4379 794
Size: 1718 1867 4798
Fragments arranged by size:
4798 1867 1718
BstBI TT'CG_AA
Cuts at: 7187 7187
Size: 8383
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8383
BstYI r'GATC_y
Cuts at: 1972 2239 2938 3256 3490 5713 6080 6465 6482
Size: 267 699 318 234 2223 367 385 17
Cuts at: 6482 7934 8020 8032 8378 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2223 1977 1452 699 385 367 346 318
267 234 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2590 2590
Size: 8383
BtgI C'CryG_G
Cuts at: 513 1065 3574 4388 5304 513
Size: 552 2509 814 916 3592
Fragments arranged by size:
3592 2509 916 814 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2507 4895 487
Size: 683 56 1281 2388 3975
Fragments arranged by size:
3975 2388 1281 683 56
BtsI GCAGTG_nn'
Cuts at: 2112 4830 4952 5025 5899 6693 6713 2112
Size: 2718 122 73 874 794 20 3782
Fragments arranged by size:
3782 2718 874 794 122 73 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8383
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8348
DraI TTT'AAA
Cuts at: 2623 2832 4864 5687 2623
Size: 209 2032 823 5319
Fragments arranged by size:
5319 2032 823 209
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7390 809
Size: 507 6074 1802
Fragments arranged by size:
6074 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5056 5830 6719
Size: 54 1425 50 275 18 3226 774 889
Cuts at: 6719 8348 8
Size: 1629 43
Fragments arranged by size:
3226 1629 1425 889 774 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5830 1537
Size: 275 4018 4090
Fragments arranged by size:
4090 4018 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2299 4113 5370 6313 1353
Size: 946 1814 1257 943 3423
Fragments arranged by size:
3423 1814 1257 946 943
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3960 6970 7344 7490 387
Size: 1064 138 2371 3010 374 146 1280
Fragments arranged by size:
3010 2371 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2085 2099 2528 2941 3108 3810 3822
Size: 393 453 14 429 413 167 702 12
Cuts at: 3822 4788 6446 7043 7830 8098 1239
Size: 966 1658 597 787 268 1524
Fragments arranged by size:
1658 1524 966 787 702 597 453 429
413 393 268 167 14 12
EcoNI CCTnn'n_nnAGG
Cuts at: 3384 3384
Size: 8383
EcoRI G'AATT_C
Cuts at: 2578 2650 3406 5692 6070 2578
Size: 72 756 2286 378 4891
Fragments arranged by size:
4891 2286 756 378 72
EcoRV GAT'ATC
Cuts at: 4066 4066
Size: 8383
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3499 5663 3499
Size: 2164 6219
FspI TGC'GCA
Cuts at: 4169 6889 4169
Size: 2720 5663
HaeII r_GCGC'y
Cuts at: 1678 7530 1678
Size: 5852 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2270 2302 2724 2756 2923
Size: 32 135 32 698 32 422 32 167
Cuts at: 2923 2955 2985 3017 3305 3337 3383 3415 3431
Size: 32 30 32 288 32 46 32 16
Cuts at: 3431 3463 3806 3838 3858 3890 7024 7056 7098
Size: 32 343 32 20 32 3134 32 42
Cuts at: 7098 7130 1373
Size: 32 2626
Fragments arranged by size:
3134 2626 698 422 343 288 167 135
46 42 32 32 32 32 32 32
32 32 32 32 32 32 32 30
20 16
HincII GTy'rAC
Cuts at: 669 1657 1938 2220 5727 5973 669
Size: 988 281 282 3507 246 3079
Fragments arranged by size:
3507 3079 988 282 281 246
HindIII A'AGCT_T
Cuts at: 748 3776 748
Size: 3028 5355
HpaI GTT'AAC
Cuts at: 5973 5973
Size: 8383
KpnI G_GTAC'C
Cuts at: 5364 5708 5364
Size: 344 8039
MfeI C'AATT_G
Cuts at: 5982 5982
Size: 8383
MluI A'CGCG_T
Cuts at: 8259 8259
Size: 8383
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4344 4785 7497 7681 1305
Size: 3039 441 2712 184 2007
Fragments arranged by size:
3039 2712 2007 441 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5058 10
Size: 54 1425 343 3226 3335
Fragments arranged by size:
3335 3226 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2778 4059 4518 5121 6341 6700 6859
Size: 648 1612 1281 459 603 1220 359 159
Cuts at: 6859 518
Size: 2042
Fragments arranged by size:
2042 1612 1281 1220 648 603 459 359
159
MspA1I CmG'CkG
Cuts at: 1606 2291 4662 5220 5799 6476 7624 7869 1606
Size: 685 2371 558 579 677 1148 245 2120
Fragments arranged by size:
2371 2120 1148 685 677 579 558 245
NcoI C'CATG_G
Cuts at: 513 1065 3574 4388 5304 513
Size: 552 2509 814 916 3592
Fragments arranged by size:
3592 2509 916 814 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8383
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8383
NotI GC'GGCC_GC
Cuts at: 5830 5830
Size: 8383
NsiI A_TGCA'T
Cuts at: 4578 4578
Size: 8383
NspI r_CATG'y
Cuts at: 1132 1229 2803 3717 5741 7286 1132
Size: 97 1574 914 2024 1545 2229
Fragments arranged by size:
2229 2024 1574 1545 914 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8383
PciI A'CATG_T
Cuts at: 7282 7282
Size: 8383
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4389 1108
Size: 3281 5102
PfoI T'CCnGG_A
Cuts at: 4765 4765
Size: 8383
PmeI GTTT'AAAC
Cuts at: 5687 5687
Size: 8383
PmlI CAC'GTG
Cuts at: 5261 5261
Size: 8383
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6452 6484 7965 7997 6452
Size: 32 1481 32 6838
Fragments arranged by size:
6838 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3678 1613
Size: 2065 6318
PsiI TTA'TAA
Cuts at: 2663 5953 2663
Size: 3290 5093
PstI C_TGCA'G
Cuts at: 830 3661 3680 4459 5020 5735 830
Size: 2831 19 779 561 715 3478
Fragments arranged by size:
3478 2831 779 715 561 19
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8383
PvuII CAG'CTG
Cuts at: 2291 4662 5220 2291
Size: 2371 558 5454
Fragments arranged by size:
5454 2371 558
SalI G'TCGA_C
Cuts at: 1936 5725 1936
Size: 3789 4594
SbfI CC_TGCA'GG
Cuts at: 5735 5735
Size: 8383
ScaI AGT'ACT
Cuts at: 5314 5646 6631 8343 5314
Size: 332 985 1712 5354
Fragments arranged by size:
5354 1712 985 332
SexAI A'CCwGG_T
Cuts at: 4276 4276
Size: 8383
SfcI C'TryA_G
Cuts at: 826 1045 2196 2724 3073 3657 3676 4455 5016
Size: 219 1151 528 349 584 19 779 561
Cuts at: 5016 5221 5731 6866 7547 7738 826
Size: 205 510 1135 681 191 1471
Fragments arranged by size:
1471 1151 1135 779 681 584 561 528
510 349 219 205 191 19
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8383
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8383
SmaI CCC'GGG
Cuts at: 1872 5710 1872
Size: 3838 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2263 4032 4438 5634 6487 7388
Size: 197 926 320 1769 406 1196 853 901
Cuts at: 7388 7650 820
Size: 262 1553
Fragments arranged by size:
1769 1553 1196 926 901 853 406 320
262 197
SnaBI TAC'GTA
Cuts at: 493 4557 6174 493
Size: 4064 1617 2702
Fragments arranged by size:
4064 2702 1617
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8383
SphI G_CATG'C
Cuts at: 1132 5741 1132
Size: 4609 3774
SspI AAT'ATT
Cuts at: 5 52 2343 3628 3932 6307 5
Size: 47 2291 1285 304 2375 2081
Fragments arranged by size:
2375 2291 2081 1285 304 47
StuI AGG'CCT
Cuts at: 1517 3602 4003 1517
Size: 2085 401 5897
Fragments arranged by size:
5897 2085 401
StyI C'CwwG_G
Cuts at: 513 1065 3574 3603 4388 4668 5034 5304 5822
Size: 552 2509 29 785 280 366 270 518
Cuts at: 5822 513
Size: 3074
Fragments arranged by size:
3074 2509 785 552 518 366 280 270
29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3624 6410 6427 6580 6765 7210 325
Size: 1255 2044 2786 17 153 185 445 1498
Fragments arranged by size:
2786 2044 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4012 5287 5312
Size: 274 80 33 51 1443 2035 1275 25
Cuts at: 5312 5644 6629 8341 96
Size: 332 985 1712 138
Fragments arranged by size:
2035 1712 1443 1275 985 332 274 138
80 51 33 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8343 8375 8343
Size: 32 8351
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8383
XbaI T'CTAG_A
Cuts at: 5719 5719
Size: 8383
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5521 5521
Size: 8383
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8383
XmaI C'CCGG_G
Cuts at: 1870 5708 1870
Size: 3838 4545
XmnI GAAnn'nnTTC
Cuts at: 2978 3562 6512 2978
Size: 584 2950 4849
Fragments arranged by size:
4849 2950 584
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6191 276
Size: 53 83 186 936 4657 2468
Fragments arranged by size:
4657 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BplI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoO109I FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SfiI
SfoI SrfI SwaI