Restriction map for
pFN21AA1033
(Circular) (Six-base) MAPSORT of: pFN21AA1033.seq Check: 9365 from: 1 to: 8224
pFN21AA1033 8224 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6034 278
Size: 53 83 186 936 4498 2468
Fragments arranged by size:
4498 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2117 4989 5567 1937
Size: 180 2872 578 4594
Fragments arranged by size:
4594 2872 578 180
Acc65I G'GTAC_C
Cuts at: 3043 5545 3043
Size: 2502 5722
AclI AA'CG_TT
Cuts at: 1563 6351 6724 1563
Size: 4788 373 3063
Fragments arranged by size:
4788 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2771 3315 5267 5389 5448 6287 7671 7939
Size: 1139 544 1952 122 59 839 1384 268
Cuts at: 7939 1632
Size: 1917
Fragments arranged by size:
1952 1917 1384 1139 839 544 268 122
59
AflII C'TTAA_G
Cuts at: 820 1017 2087 820
Size: 197 1070 6957
Fragments arranged by size:
6957 1070 197
AflIII A'CryG_T
Cuts at: 1203 3387 3900 7123 8100 1203
Size: 2184 513 3223 977 1327
Fragments arranged by size:
3223 2184 1327 977 513
AgeI A'CCGG_T
Cuts at: 7055 7055
Size: 8224
AhdI GACnn_n'nnGTC
Cuts at: 5345 6953 5345
Size: 1608 6616
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8224
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8224
AlwNI CAG_nnn'CTG
Cuts at: 2443 3116 5967 7539 2443
Size: 673 2851 1572 3128
Fragments arranged by size:
3128 2851 1572 673
ApaLI G'TGCA_C
Cuts at: 6281 7437 6281
Size: 1156 7068
ApoI r'AATT_y
Cuts at: 1493 2067 2102 2336 2406 2702 2709 2864 2955
Size: 574 35 234 70 296 7 155 91
Cuts at: 2955 3287 4002 4676 4858 4951 5006 5038 5360
Size: 332 715 674 182 93 55 32 322
Cuts at: 5360 5397 5533 5759 5911 8020 1493
Size: 37 136 226 152 2109 1697
Fragments arranged by size:
2109 1697 715 674 574 332 322 296
234 226 182 155 152 136 93 91
70 55 37 35 32 7
AseI AT'TA_AT
Cuts at: 160 1032 4458 6778 160
Size: 872 3426 2320 1606
Fragments arranged by size:
3426 2320 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8224
AvaI C'yCGr_G
Cuts at: 1870 1943 5549 1870
Size: 73 3606 4545
Fragments arranged by size:
4545 3606 73
BamHI G'GATC_C
Cuts at: 5554 5921 5554
Size: 367 7857
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3043 5545 7000 618
Size: 325 217 627 1256 2502 1455 1842
Fragments arranged by size:
2502 1842 1455 1256 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3236 5543 1391
Size: 1845 2307 4072
Fragments arranged by size:
4072 2307 1845
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3134 3832 4796 928
Size: 971 1235 698 964 4356
Fragments arranged by size:
4356 1235 971 964 698
BbvCI CC'TCA_GC
Cuts at: 2859 2859
Size: 8224
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6415 6449 1903
Size: 34 4478 34 3678
Fragments arranged by size:
4478 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3160 4665 6116 7326 3160
Size: 1505 1451 1210 4058
Fragments arranged by size:
4058 1505 1451 1210
BclI T'GATC_A
Cuts at: 1548 4645 4732 4939 1548
Size: 3097 87 207 4833
Fragments arranged by size:
4833 3097 207 87
BfrBI ATG'CAT
Cuts at: 3344 3940 3344
Size: 596 7628
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6835 136
Size: 107 122 71 631 605 5163 1525
Fragments arranged by size:
5163 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3298 4051 4793 8219 3298
Size: 753 742 3426 3303
Fragments arranged by size:
3426 3303 753 742
BlpI GC'TnA_GC
Cuts at: 5268 5641 5268
Size: 373 7851
Bme1580I G_kGCm'C
Cuts at: 1790 6285 7441 1790
Size: 4495 1156 2573
Fragments arranged by size:
4495 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8224
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2041 4255 4314
Size: 416 53 83 399 24 624 2214 59
Cuts at: 4314 5955 6913 442
Size: 1641 958 1753
Fragments arranged by size:
2214 1753 1641 958 624 416 399 83
59 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8224
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2137 2137
Size: 8224
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2571 4986 6884 1239
Size: 1332 2415 1898 2579
Fragments arranged by size:
2579 2415 1898 1332
Bpu10I CC'TnA_GC
Cuts at: 2859 2859
Size: 8224
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2129 3437 6349 7214 7512 2129
Size: 1308 2912 865 298 2841
Fragments arranged by size:
2912 2841 1308 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2354 5475 6887 882
Size: 617 855 3121 1412 2219
Fragments arranged by size:
3121 2219 1412 855 617
BsaAI yAC'GTr
Cuts at: 493 1204 4189 6015 493
Size: 711 2985 1826 2702
Fragments arranged by size:
2985 2702 1826 711
BsaBI GATnn'nnATC
Cuts at: 2181 5193 5349 8218 2181
Size: 3012 156 2869 2187
Fragments arranged by size:
3012 2869 2187 156
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6031 6413 7048 275
Size: 53 83 186 936 4498 382 635 1451
Fragments arranged by size:
4498 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6657 7055 7329 7476 7887 6657
Size: 398 274 147 411 6994
Fragments arranged by size:
6994 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2006 2036 2820 2850 1178
Size: 30 165 30 603 30 784 30 6552
Fragments arranged by size:
6552 784 603 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2126 2841 1186
Size: 940 715 6569
Fragments arranged by size:
6569 940 715
BseYI C'CCAG_C
Cuts at: 7427 7923 7427
Size: 496 7728
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2274 3454 1163
Size: 573 538 1180 5933
Fragments arranged by size:
5933 1180 573 538
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5674 6435 7463 1540
Size: 275 186 3673 761 1028 2301
Fragments arranged by size:
3673 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2580 3236 3615 6285 6370 7441 2580
Size: 656 379 2670 85 1071 3363
Fragments arranged by size:
3363 2670 1071 656 379 85
BsmI GAATG_Cn'
Cuts at: 5083 5735 5828 5083
Size: 652 93 7479
Fragments arranged by size:
7479 652 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2580 3236 3615 5543 6285 6370 7441
Size: 399 790 656 379 1928 742 85 1071
Cuts at: 7441 1391
Size: 2174
Fragments arranged by size:
2174 1928 1071 790 742 656 399 379
85
BspEI T'CCGG_A
Cuts at: 7887 7887
Size: 8224
BspHI T'CATG_A
Cuts at: 3753 4240 6113 7843 3753
Size: 487 1873 1730 4134
Fragments arranged by size:
4134 1873 1730 487
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3937 5579 844
Size: 3093 1642 3489
Fragments arranged by size:
3489 3093 1642
BsrBI CCG'CTC
Cuts at: 1394 1823 6111 1394
Size: 429 4288 3507
Fragments arranged by size:
4288 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4825 5482 6719 6893 65
Size: 1183 3577 657 1237 174 1396
Fragments arranged by size:
3577 1396 1237 1183 657 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6868 7055 1700
Size: 110 5058 187 2869
Fragments arranged by size:
5058 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2661 3208 5281 96
Size: 2565 547 2073 3039
Fragments arranged by size:
3039 2565 2073 547
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8224
BssSI C'ACGA_G
Cuts at: 6284 7296 6284
Size: 1012 7212
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8224
BstBI TT'CG_AA
Cuts at: 2992 4486 5011 7028 2992
Size: 1494 525 2017 4188
Fragments arranged by size:
4188 2017 1494 525
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8224
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2389 5164 2011
Size: 378 2775 5071
Fragments arranged by size:
5071 2775 378
BstYI r'GATC_y
Cuts at: 1972 3298 3766 4051 4793 5554 5921 6306 6323
Size: 1326 468 285 742 761 367 385 17
Cuts at: 6323 7775 7861 7873 8219 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1326 761 742 468 385 367
346 285 86 17 12
BstZ17I GTA'TAC
Cuts at: 2118 2118
Size: 8224
Bsu36I CC'TnA_GG
Cuts at: 4713 4713
Size: 8224
BtgI C'CryG_G
Cuts at: 513 1065 2004 5157 513
Size: 552 939 3153 3580
Fragments arranged by size:
3580 3153 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7485
Fragments arranged by size:
7485 683 56
BtsI GCAGTG_nn'
Cuts at: 5395 5740 6534 6554 5395
Size: 345 794 20 7065
Fragments arranged by size:
7065 794 345 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8224
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8189
DraI TTT'AAA
Cuts at: 3721 4008 4080 5528 3721
Size: 287 72 1448 6417
Fragments arranged by size:
6417 1448 287 72
DraIII CAC_nnn'GTG
Cuts at: 3695 3806 3695
Size: 111 8113
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7231 809
Size: 507 5915 1802
Fragments arranged by size:
5915 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3558 5671 6560
Size: 54 1425 50 275 18 1728 2113 889
Cuts at: 6560 8189 8
Size: 1629 43
Fragments arranged by size:
2113 1728 1629 1425 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5671 1537
Size: 275 3859 4090
Fragments arranged by size:
4090 3859 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3546 6154 1353
Size: 2193 2608 3423
Fragments arranged by size:
3423 2608 2193
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6811 7185 7331 387
Size: 1064 138 5222 374 146 1280
Fragments arranged by size:
5222 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3234 3234
Size: 8224
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2571 2771 3315 4986 5267 5389 5448
Size: 393 939 200 544 1671 281 122 59
Cuts at: 5448 6287 6884 7671 7939 1239
Size: 839 597 787 268 1524
Fragments arranged by size:
1671 1524 939 839 787 597 544 393
281 268 200 122 59
EcoO109I rG'GnC_Cy
Cuts at: 4339 4339
Size: 8224
EcoRI G'AATT_C
Cuts at: 2709 5533 5911 2709
Size: 2824 378 5022
Fragments arranged by size:
5022 2824 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2102 2927 4904 2102
Size: 825 1977 5422
Fragments arranged by size:
5422 1977 825
FspI TGC'GCA
Cuts at: 6730 6730
Size: 8224
HaeII r_GCGC'y
Cuts at: 1678 7371 1678
Size: 5693 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2006 2038 2042 2074 3197
Size: 32 135 32 434 32 4 32 1123
Cuts at: 3197 3229 3599 3631 3777 3809 5331 5363 6865
Size: 32 370 32 146 32 1522 32 1502
Cuts at: 6865 6897 6939 6971 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1522 1502 1123 434 370 146 135
42 32 32 32 32 32 32 32
32 32 32 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2050 5568 5814 669
Size: 988 281 112 3518 246 3079
Fragments arranged by size:
3518 3079 988 281 246 112
HindIII A'AGCT_T
Cuts at: 748 2204 5180 748
Size: 1456 2976 3792
Fragments arranged by size:
3792 2976 1456
HpaI GTT'AAC
Cuts at: 5814 5814
Size: 8224
KpnI G_GTAC'C
Cuts at: 3047 5549 3047
Size: 2502 5722
MfeI C'AATT_G
Cuts at: 2160 5823 2160
Size: 3663 4561
MluI A'CGCG_T
Cuts at: 8100 8100
Size: 8224
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2107 2932 7338 7522 1305
Size: 802 825 4406 184 2007
Fragments arranged by size:
4406 2007 825 802 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3560 10
Size: 54 1425 343 1728 4674
Fragments arranged by size:
4674 1728 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3392 3491 3707 5162 6182 6541
Size: 648 843 1383 99 216 1455 1020 359
Cuts at: 6541 6700 518
Size: 159 2042
Fragments arranged by size:
2042 1455 1383 1020 843 648 359 216
159 99
MspA1I CmG'CkG
Cuts at: 1606 3116 5640 6317 7465 7710 1606
Size: 1510 2524 677 1148 245 2120
Fragments arranged by size:
2524 2120 1510 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 5157 513
Size: 552 939 3153 3580
Fragments arranged by size:
3580 3153 939 552
NdeI CA'TA_TG
Cuts at: 387 3468 387
Size: 3081 5143
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8224
NotI GC'GGCC_GC
Cuts at: 5671 5671
Size: 8224
NsiI A_TGCA'T
Cuts at: 3346 3942 3346
Size: 596 7628
NspI r_CATG'y
Cuts at: 1132 1229 3391 3904 3944 5582 7127 1132
Size: 97 2162 513 40 1638 1545 2229
Fragments arranged by size:
2229 2162 1638 1545 513 97 40
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8224
PciI A'CATG_T
Cuts at: 3387 3900 7123 3387
Size: 513 3223 4488
Fragments arranged by size:
4488 3223 513
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2497 1108
Size: 1389 6835
PmeI GTTT'AAAC
Cuts at: 5528 5528
Size: 8224
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3455 3487 6293 6325 7806 7838 3455
Size: 32 2806 32 1481 32 3841
Fragments arranged by size:
3841 2806 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8224
PsiI TTA'TAA
Cuts at: 3015 5794 3015
Size: 2779 5445
PstI C_TGCA'G
Cuts at: 830 5576 830
Size: 4746 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8224
PvuII CAG'CTG
Cuts at: 3116 3116
Size: 8224
SacI G_AGCT'C
Cuts at: 3236 3236
Size: 8224
SalI G'TCGA_C
Cuts at: 1936 5566 1936
Size: 3630 4594
SapI GCTCTTCn'nnn_
Cuts at: 3546 3546
Size: 8224
SbfI CC_TGCA'GG
Cuts at: 5576 5576
Size: 8224
ScaI AGT'ACT
Cuts at: 2851 6472 8184 2851
Size: 3621 1712 2891
Fragments arranged by size:
3621 2891 1712
SfcI C'TryA_G
Cuts at: 826 1045 2193 2373 3475 4230 5354 5572 6707
Size: 219 1148 180 1102 755 1124 218 1135
Cuts at: 6707 7388 7579 826
Size: 681 191 1471
Fragments arranged by size:
1471 1148 1135 1124 1102 755 681 219
218 191 180
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8224
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8224
SmaI CCC'GGG
Cuts at: 1872 5551 1872
Size: 3679 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2087 2108 3452 6328 7229 7491
Size: 197 926 144 21 1344 2876 901 262
Cuts at: 7491 820
Size: 1553
Fragments arranged by size:
2876 1553 1344 926 901 262 197 144
21
SnaBI TAC'GTA
Cuts at: 493 4189 6015 493
Size: 3696 1826 2702
Fragments arranged by size:
3696 2702 1826
SpeI A'CTAG_T
Cuts at: 152 4870 152
Size: 4718 3506
SphI G_CATG'C
Cuts at: 1132 3944 5582 1132
Size: 2812 1638 3774
Fragments arranged by size:
3774 2812 1638
SspI AAT'ATT
Cuts at: 5 52 3070 4462 4973 6148 5
Size: 47 3018 1392 511 1175 2081
Fragments arranged by size:
3018 2081 1392 1175 511 47
StuI AGG'CCT
Cuts at: 1517 2792 1517
Size: 1275 6949
StyI C'CwwG_G
Cuts at: 513 1065 2004 5157 5663 513
Size: 552 939 3153 506 3074
Fragments arranged by size:
3153 3074 939 552 506
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2545 2871 4107 5150 6251 6268 6421
Size: 1255 965 326 1236 1043 1101 17 153
Cuts at: 6421 6606 7051 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1236 1101 1043 965 445 326
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2661 2849 3208
Size: 274 80 33 51 1443 684 188 359
Cuts at: 3208 5281 6470 8182 96
Size: 2073 1189 1712 138
Fragments arranged by size:
2073 1712 1443 1189 684 359 274 188
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2820 2852 4577 4609 8184 8216 2820
Size: 32 1725 32 3575 32 2828
Fragments arranged by size:
3575 2828 1725 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2020 1316
Size: 704 7520
XbaI T'CTAG_A
Cuts at: 5560 5560
Size: 8224
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4327 4327
Size: 8224
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8224
XmaI C'CCGG_G
Cuts at: 1870 5549 1870
Size: 3679 4545
XmnI GAAnn'nnTTC
Cuts at: 3735 6353 3735
Size: 2618 5606
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6032 276
Size: 53 83 186 936 4498 2468
Fragments arranged by size:
4498 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BsiWI BsmBI EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI