(Circular) (Six-base) MAPSORT of: pFN21AA1023.seq Check: 2435 from: 1 to: 6595 pFN21AA1023 6595 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3831 4405 278 Size: 53 83 186 936 2295 574 2468 Fragments arranged by size: 2468 2295 936 574 186 83 53 AccI GT'mk_AC Cuts at: 1937 2226 3938 1937 Size: 289 1712 4594 Fragments arranged by size: 4594 1712 289 Acc65I G'GTAC_C Cuts at: 3916 3916 Size: 6595 AclI AA'CG_TT Cuts at: 1563 4722 5095 1563 Size: 3159 373 3063 Fragments arranged by size: 3159 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2146 2474 2489 2762 3029 3440 3711 4658 Size: 514 328 15 273 267 411 271 947 Cuts at: 4658 6042 6310 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 947 514 411 328 273 271 268 267 15 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6398 AflIII A'CryG_T Cuts at: 1203 5494 6471 1203 Size: 4291 977 1327 Fragments arranged by size: 4291 1327 977 AgeI A'CCGG_T Cuts at: 3680 5426 3680 Size: 1746 4849 AhdI GACnn_n'nnGTC Cuts at: 5324 5324 Size: 6595 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6595 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3623 1184 Size: 2439 4156 AlwNI CAG_nnn'CTG Cuts at: 2044 2086 2374 2740 3043 3073 3288 4338 5910 Size: 42 288 366 303 30 215 1050 1572 Cuts at: 5910 2044 Size: 2729 Fragments arranged by size: 2729 1572 1050 366 303 288 215 42 30 ApaLI G'TGCA_C Cuts at: 4652 5808 4652 Size: 1156 5439 ApoI r'AATT_y Cuts at: 1493 3904 4130 4282 6391 1493 Size: 2411 226 152 2109 1697 Fragments arranged by size: 2411 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5149 160 Size: 872 4117 1606 Fragments arranged by size: 4117 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6595 AvaI C'yCGr_G Cuts at: 1870 1943 3660 3920 1870 Size: 73 1717 260 4545 Fragments arranged by size: 4545 1717 260 73 BamHI G'GATC_C Cuts at: 2377 2463 3925 4292 2377 Size: 86 1462 367 4680 Fragments arranged by size: 4680 1462 367 86 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2325 2501 2657 3808 3916 Size: 325 217 627 538 176 156 1151 108 Cuts at: 3916 5371 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1151 627 538 325 217 176 156 108 BanII G_rGCy'C Cuts at: 1391 2721 2876 3261 3330 3557 3581 3914 1391 Size: 1330 155 385 69 227 24 333 4072 Fragments arranged by size: 4072 1330 385 333 227 155 69 24 BbeI G_GCGC'C Cuts at: 2329 2661 2329 Size: 332 6263 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2537 2657 928 Size: 971 638 120 4866 Fragments arranged by size: 4866 971 638 120 BbvCI CC'TCA_GC Cuts at: 2828 2828 Size: 6595 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4786 4820 1903 Size: 34 50 34 2765 34 3678 Fragments arranged by size: 3678 2765 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4487 5697 4487 Size: 1210 5385 BclI T'GATC_A Cuts at: 1548 1548 Size: 6595 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5206 136 Size: 107 122 71 631 605 3534 1525 Fragments arranged by size: 3534 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6590 6590 Size: 6595 BlpI GC'TnA_GC Cuts at: 2765 4012 2765 Size: 1247 5348 Bme1580I G_kGCm'C Cuts at: 1790 2689 3567 3652 4656 5812 1790 Size: 899 878 85 1004 1156 2573 Fragments arranged by size: 2573 1156 1004 899 878 85 BmgBI CAC'GTC Cuts at: 1318 2299 1318 Size: 981 5614 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3388 4326 5284 Size: 416 53 83 399 24 1971 938 958 Cuts at: 5284 442 Size: 1753 Fragments arranged by size: 1971 1753 958 938 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6595 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2351 2501 2579 2867 3113 3125 5255 1239 Size: 1112 150 78 288 246 12 2130 2579 Fragments arranged by size: 2579 2130 1112 288 246 150 78 12 Bpu10I CC'TnA_GC Cuts at: 2693 2828 2693 Size: 135 6460 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4720 5585 5883 4720 Size: 865 298 5432 Fragments arranged by size: 5432 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2997 5258 882 Size: 617 1498 2261 2219 Fragments arranged by size: 2261 2219 1498 617 BsaAI yAC'GTr Cuts at: 493 1204 2284 4386 493 Size: 711 1080 2102 2702 Fragments arranged by size: 2702 2102 1080 711 BsaBI GATnn'nnATC Cuts at: 2555 6589 2555 Size: 4034 2561 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2124 2326 2658 3369 Size: 53 83 186 936 591 202 332 711 Cuts at: 3369 3744 3828 4402 4784 5419 275 Size: 375 84 574 382 635 1451 Fragments arranged by size: 1451 936 711 635 591 574 382 375 332 202 186 84 83 53 BsaWI w'CCGG_w Cuts at: 2624 3680 5028 5426 5700 5847 6258 2624 Size: 1056 1348 398 274 147 411 2961 Fragments arranged by size: 2961 1348 1056 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2099 2129 3092 3122 3734 Size: 30 165 30 696 30 963 30 612 Cuts at: 3734 3764 3818 3848 1178 Size: 30 54 30 3925 Fragments arranged by size: 3925 963 696 612 165 54 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2348 2594 3140 3143 3148 3161 3176 3335 Size: 1162 246 546 3 5 13 15 159 Cuts at: 3335 3629 3730 3739 1186 Size: 294 101 9 4042 Fragments arranged by size: 4042 1162 546 294 246 159 101 15 13 9 5 3 BseYI C'CCAG_C Cuts at: 2050 2381 3262 3275 3527 5798 6294 2050 Size: 331 881 13 252 2271 496 2351 Fragments arranged by size: 2351 2271 881 496 331 252 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2306 2927 3629 1163 Size: 573 570 621 702 4129 Fragments arranged by size: 4129 702 621 573 570 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2020 4045 4806 5834 1540 Size: 275 186 19 2025 761 1028 2301 Fragments arranged by size: 2301 2025 1028 761 275 186 19 BsiHKAI G_wGCw'C Cuts at: 2721 2771 2876 3261 3557 4656 4741 5812 2721 Size: 50 105 385 296 1099 85 1071 3504 Fragments arranged by size: 3504 1099 1071 385 296 105 85 50 BsmI GAATG_Cn' Cuts at: 4106 4199 4106 Size: 93 6502 BsmBI CGTCTCn'nnnn_ Cuts at: 2597 2990 3360 3873 2597 Size: 393 370 513 5319 Fragments arranged by size: 5319 513 393 370 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2689 2721 2771 2876 3261 3330 3557 Size: 399 899 32 50 105 385 69 227 Cuts at: 3557 3567 3581 3652 3914 4656 4741 5812 1391 Size: 10 14 71 262 742 85 1071 2174 Fragments arranged by size: 2174 1071 899 742 399 385 262 227 105 85 71 69 50 32 14 10 BspEI T'CCGG_A Cuts at: 6258 6258 Size: 6595 BspHI T'CATG_A Cuts at: 4484 6214 4484 Size: 1730 4865 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3950 844 Size: 3106 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3019 3342 3641 4482 1394 Size: 429 1196 323 299 841 3507 Fragments arranged by size: 3507 1196 841 429 323 299 BsrDI GCAATG_nn' Cuts at: 65 1248 2276 3858 5090 5264 65 Size: 1183 1028 1582 1232 174 1396 Fragments arranged by size: 1582 1396 1232 1183 1028 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2355 3680 5239 5426 1700 Size: 110 545 1325 1559 187 2869 Fragments arranged by size: 2869 1559 1325 545 187 110 BsrGI T'GTAC_A Cuts at: 96 3405 96 Size: 3309 3286 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6595 BssSI C'ACGA_G Cuts at: 2955 2978 4655 5667 2955 Size: 23 1677 1012 3883 Fragments arranged by size: 3883 1677 1012 23 BstAPI GCAn_nnn'nTGC Cuts at: 794 3043 3073 3288 794 Size: 2249 30 215 4101 Fragments arranged by size: 4101 2249 215 30 BstBI TT'CG_AA Cuts at: 5399 5399 Size: 6595 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6595 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6595 BstYI r'GATC_y Cuts at: 1972 2377 2395 2463 3925 4292 4677 4694 6146 Size: 405 18 68 1462 367 385 17 1452 Cuts at: 6146 6232 6244 6590 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1462 1452 405 385 367 346 86 68 18 17 12 Bsu36I CC'TnA_GG Cuts at: 3790 3790 Size: 6595 BtgI C'CryG_G Cuts at: 513 1065 2386 2562 3332 3372 3594 513 Size: 552 1321 176 770 40 222 3514 Fragments arranged by size: 3514 1321 770 552 222 176 40 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3568 487 Size: 683 56 2342 3514 Fragments arranged by size: 3514 2342 683 56 BtsI GCAGTG_nn' Cuts at: 2489 2689 3286 4111 4905 4925 2489 Size: 200 597 825 794 20 4159 Fragments arranged by size: 4159 825 794 597 200 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6595 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3786 3821 529 Size: 35 3222 35 3303 Fragments arranged by size: 3303 3222 35 35 DraI TTT'AAA Cuts at: 3899 3899 Size: 6595 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3854 5602 809 Size: 507 2538 1748 1802 Fragments arranged by size: 2538 1802 1748 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2017 3302 3375 Size: 54 1425 50 275 18 187 1285 73 Cuts at: 3375 4042 4931 6560 8 Size: 667 889 1629 43 Fragments arranged by size: 1629 1425 1285 889 667 275 187 73 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2017 4042 1537 Size: 275 205 2025 4090 Fragments arranged by size: 4090 2025 275 205 EarI CTCTTCn'nnn_ Cuts at: 1353 2165 2285 2541 2721 3009 3210 3358 3777 Size: 812 120 256 180 288 201 148 419 Cuts at: 3777 4525 1353 Size: 748 3423 Fragments arranged by size: 3423 812 748 419 288 256 201 180 148 120 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5182 5556 5702 387 Size: 1064 138 3593 374 146 1280 Fragments arranged by size: 3593 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2719 2874 3259 3555 2719 Size: 155 385 296 5759 Fragments arranged by size: 5759 385 296 155 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2146 2351 2474 2489 2501 2579 2762 Size: 393 514 205 123 15 12 78 183 Cuts at: 2762 2867 3029 3113 3125 3440 3711 4658 5255 Size: 105 162 84 12 315 271 947 597 Cuts at: 5255 6042 6310 1239 Size: 787 268 1524 Fragments arranged by size: 1524 947 787 597 514 393 315 271 268 205 183 162 123 105 84 78 15 12 12 EcoNI CCTnn'n_nnAGG Cuts at: 2333 2744 3095 2333 Size: 411 351 5833 Fragments arranged by size: 5833 411 351 EcoO109I rG'GnC_Cy Cuts at: 2083 2749 2083 Size: 666 5929 EcoRI G'AATT_C Cuts at: 3904 4282 3904 Size: 378 6217 FspI TGC'GCA Cuts at: 5101 5101 Size: 6595 HaeII r_GCGC'y Cuts at: 1678 2191 2329 2661 5742 1678 Size: 513 138 332 3081 2531 Fragments arranged by size: 3081 2531 513 332 138 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2164 2196 3539 3571 3734 Size: 32 135 32 592 32 1343 32 163 Cuts at: 3734 3766 3818 3850 5236 5268 5310 5342 1373 Size: 32 52 32 1386 32 42 32 2626 Fragments arranged by size: 2626 1386 1343 592 163 135 52 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3832 3939 4185 669 Size: 988 281 1894 107 246 3079 Fragments arranged by size: 3079 1894 988 281 246 107 HindIII A'AGCT_T Cuts at: 748 748 Size: 6595 HpaI GTT'AAC Cuts at: 4185 4185 Size: 6595 KasI G'GCGC_C Cuts at: 2325 2657 2325 Size: 332 6263 KpnI G_GTAC'C Cuts at: 3920 3920 Size: 6595 MfeI C'AATT_G Cuts at: 4194 4194 Size: 6595 MluI A'CGCG_T Cuts at: 6471 6471 Size: 6595 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3870 5709 5893 1305 Size: 2565 1839 184 2007 Fragments arranged by size: 2565 2007 1839 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4773 Fragments arranged by size: 4773 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4553 4912 5071 518 Size: 648 3387 359 159 2042 Fragments arranged by size: 3387 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2346 2388 3040 3334 3374 4011 4688 5836 Size: 740 42 652 294 40 637 677 1148 Cuts at: 5836 6081 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 740 677 652 637 294 245 42 40 NarI GG'CG_CC Cuts at: 2326 2658 2326 Size: 332 6263 NcoI C'CATG_G Cuts at: 513 1065 2562 513 Size: 552 1497 4546 Fragments arranged by size: 4546 1497 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6595 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6595 NotI GC'GGCC_GC Cuts at: 4042 4042 Size: 6595 NspI r_CATG'y Cuts at: 1132 1229 2114 3953 5498 1132 Size: 97 885 1839 1545 2229 Fragments arranged by size: 2229 1839 1545 885 97 PasI CC'CwG_GG Cuts at: 1348 2213 2638 1348 Size: 865 425 5305 Fragments arranged by size: 5305 865 425 PciI A'CATG_T Cuts at: 5494 5494 Size: 6595 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6595 PfoI T'CCnGG_A Cuts at: 2373 2702 2712 2373 Size: 329 10 6256 Fragments arranged by size: 6256 329 10 PmeI GTTT'AAAC Cuts at: 3899 3899 Size: 6595 PmlI CAC'GTG Cuts at: 2284 2284 Size: 6595 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4664 4696 6177 6209 4664 Size: 32 1481 32 5050 Fragments arranged by size: 5050 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2749 2749 Size: 6595 PshAI GACnn'nnGTC Cuts at: 1613 2123 2222 1613 Size: 510 99 5986 Fragments arranged by size: 5986 510 99 PsiI TTA'TAA Cuts at: 4165 4165 Size: 6595 PstI C_TGCA'G Cuts at: 830 3033 3048 3078 3390 3947 830 Size: 2203 15 30 312 557 3478 Fragments arranged by size: 3478 2203 557 312 30 15 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6595 PvuII CAG'CTG Cuts at: 3040 3040 Size: 6595 RsrII CG'GwC_CG Cuts at: 3021 3021 Size: 6595 SacI G_AGCT'C Cuts at: 2721 2876 3261 3557 2721 Size: 155 385 296 5759 Fragments arranged by size: 5759 385 296 155 SacII CC_GC'GG Cuts at: 2389 3335 3375 2389 Size: 946 40 5609 Fragments arranged by size: 5609 946 40 SalI G'TCGA_C Cuts at: 1936 3937 1936 Size: 2001 4594 SapI GCTCTTCn'nnn_ Cuts at: 2285 3009 3777 2285 Size: 724 768 5103 Fragments arranged by size: 5103 768 724 SbfI CC_TGCA'GG Cuts at: 3078 3390 3947 3078 Size: 312 557 5726 Fragments arranged by size: 5726 557 312 ScaI AGT'ACT Cuts at: 4843 6555 4843 Size: 1712 4883 SfcI C'TryA_G Cuts at: 826 1045 2227 3029 3044 3074 3386 3943 5078 Size: 219 1182 802 15 30 312 557 1135 Cuts at: 5078 5759 5950 826 Size: 681 191 1471 Fragments arranged by size: 1471 1182 1135 802 681 557 312 219 191 30 15 SfoI GGC'GCC Cuts at: 2327 2659 2327 Size: 332 6263 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6595 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6595 SmaI CCC'GGG Cuts at: 1872 3662 3922 1872 Size: 1790 260 4545 Fragments arranged by size: 4545 1790 260 SmlI C'TyrA_G Cuts at: 820 1017 1943 4699 5600 5862 820 Size: 197 926 2756 901 262 1553 Fragments arranged by size: 2756 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4386 493 Size: 3893 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6595 SphI G_CATG'C Cuts at: 1132 2114 3953 1132 Size: 982 1839 3774 Fragments arranged by size: 3774 1839 982 SrfI GCCC'GGGC Cuts at: 3662 3662 Size: 6595 SspI AAT'ATT Cuts at: 5 52 4519 5 Size: 47 4467 2081 Fragments arranged by size: 4467 2081 47 StuI AGG'CCT Cuts at: 1517 3540 3803 1517 Size: 2023 263 4309 Fragments arranged by size: 4309 2023 263 StyI C'CwwG_G Cuts at: 513 1065 2562 3804 4034 513 Size: 552 1497 1242 230 3074 Fragments arranged by size: 3074 1497 1242 552 230 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2537 3541 4622 4639 4792 4977 5422 Size: 1255 957 1004 1081 17 153 185 445 Cuts at: 5422 325 Size: 1498 Fragments arranged by size: 1498 1255 1081 1004 957 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3405 4841 6553 Size: 274 80 33 51 1443 1428 1436 1712 Cuts at: 6553 96 Size: 138 Fragments arranged by size: 1712 1443 1436 1428 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2099 2131 2468 2500 3092 3124 6555 6587 2099 Size: 32 337 32 592 32 3431 32 2107 Fragments arranged by size: 3431 2107 592 337 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6595 XbaI T'CTAG_A Cuts at: 3931 3931 Size: 6595 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6595 XmaI C'CCGG_G Cuts at: 1870 3660 3920 1870 Size: 1790 260 4545 Fragments arranged by size: 4545 1790 260 XmnI GAAnn'nnTTC Cuts at: 4724 4724 Size: 6595 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3829 4403 276 Size: 53 83 186 936 2295 574 2468 Fragments arranged by size: 2468 2295 936 574 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI SanDI SexAI SfiI SwaI XcmI