Restriction map for
pFN21AA1023
(Circular) (Six-base) MAPSORT of: pFN21AA1023.seq Check: 2435 from: 1 to: 6595
pFN21AA1023 6595 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3831 4405 278
Size: 53 83 186 936 2295 574 2468
Fragments arranged by size:
2468 2295 936 574 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2226 3938 1937
Size: 289 1712 4594
Fragments arranged by size:
4594 1712 289
Acc65I G'GTAC_C
Cuts at: 3916 3916
Size: 6595
AclI AA'CG_TT
Cuts at: 1563 4722 5095 1563
Size: 3159 373 3063
Fragments arranged by size:
3159 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2146 2474 2489 2762 3029 3440 3711 4658
Size: 514 328 15 273 267 411 271 947
Cuts at: 4658 6042 6310 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 947 514 411 328 273 271
268 267 15
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6398
AflIII A'CryG_T
Cuts at: 1203 5494 6471 1203
Size: 4291 977 1327
Fragments arranged by size:
4291 1327 977
AgeI A'CCGG_T
Cuts at: 3680 5426 3680
Size: 1746 4849
AhdI GACnn_n'nnGTC
Cuts at: 5324 5324
Size: 6595
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6595
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3623 1184
Size: 2439 4156
AlwNI CAG_nnn'CTG
Cuts at: 2044 2086 2374 2740 3043 3073 3288 4338 5910
Size: 42 288 366 303 30 215 1050 1572
Cuts at: 5910 2044
Size: 2729
Fragments arranged by size:
2729 1572 1050 366 303 288 215 42
30
ApaLI G'TGCA_C
Cuts at: 4652 5808 4652
Size: 1156 5439
ApoI r'AATT_y
Cuts at: 1493 3904 4130 4282 6391 1493
Size: 2411 226 152 2109 1697
Fragments arranged by size:
2411 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5149 160
Size: 872 4117 1606
Fragments arranged by size:
4117 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6595
AvaI C'yCGr_G
Cuts at: 1870 1943 3660 3920 1870
Size: 73 1717 260 4545
Fragments arranged by size:
4545 1717 260 73
BamHI G'GATC_C
Cuts at: 2377 2463 3925 4292 2377
Size: 86 1462 367 4680
Fragments arranged by size:
4680 1462 367 86
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2325 2501 2657 3808 3916
Size: 325 217 627 538 176 156 1151 108
Cuts at: 3916 5371 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1151 627 538 325 217 176
156 108
BanII G_rGCy'C
Cuts at: 1391 2721 2876 3261 3330 3557 3581 3914 1391
Size: 1330 155 385 69 227 24 333 4072
Fragments arranged by size:
4072 1330 385 333 227 155 69 24
BbeI G_GCGC'C
Cuts at: 2329 2661 2329
Size: 332 6263
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2537 2657 928
Size: 971 638 120 4866
Fragments arranged by size:
4866 971 638 120
BbvCI CC'TCA_GC
Cuts at: 2828 2828
Size: 6595
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4786 4820 1903
Size: 34 50 34 2765 34 3678
Fragments arranged by size:
3678 2765 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4487 5697 4487
Size: 1210 5385
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6595
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5206 136
Size: 107 122 71 631 605 3534 1525
Fragments arranged by size:
3534 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6590 6590
Size: 6595
BlpI GC'TnA_GC
Cuts at: 2765 4012 2765
Size: 1247 5348
Bme1580I G_kGCm'C
Cuts at: 1790 2689 3567 3652 4656 5812 1790
Size: 899 878 85 1004 1156 2573
Fragments arranged by size:
2573 1156 1004 899 878 85
BmgBI CAC'GTC
Cuts at: 1318 2299 1318
Size: 981 5614
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3388 4326 5284
Size: 416 53 83 399 24 1971 938 958
Cuts at: 5284 442
Size: 1753
Fragments arranged by size:
1971 1753 958 938 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6595
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2351 2501 2579 2867 3113 3125 5255 1239
Size: 1112 150 78 288 246 12 2130 2579
Fragments arranged by size:
2579 2130 1112 288 246 150 78 12
Bpu10I CC'TnA_GC
Cuts at: 2693 2828 2693
Size: 135 6460
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4720 5585 5883 4720
Size: 865 298 5432
Fragments arranged by size:
5432 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2997 5258 882
Size: 617 1498 2261 2219
Fragments arranged by size:
2261 2219 1498 617
BsaAI yAC'GTr
Cuts at: 493 1204 2284 4386 493
Size: 711 1080 2102 2702
Fragments arranged by size:
2702 2102 1080 711
BsaBI GATnn'nnATC
Cuts at: 2555 6589 2555
Size: 4034 2561
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2124 2326 2658 3369
Size: 53 83 186 936 591 202 332 711
Cuts at: 3369 3744 3828 4402 4784 5419 275
Size: 375 84 574 382 635 1451
Fragments arranged by size:
1451 936 711 635 591 574 382 375
332 202 186 84 83 53
BsaWI w'CCGG_w
Cuts at: 2624 3680 5028 5426 5700 5847 6258 2624
Size: 1056 1348 398 274 147 411 2961
Fragments arranged by size:
2961 1348 1056 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2099 2129 3092 3122 3734
Size: 30 165 30 696 30 963 30 612
Cuts at: 3734 3764 3818 3848 1178
Size: 30 54 30 3925
Fragments arranged by size:
3925 963 696 612 165 54 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2348 2594 3140 3143 3148 3161 3176 3335
Size: 1162 246 546 3 5 13 15 159
Cuts at: 3335 3629 3730 3739 1186
Size: 294 101 9 4042
Fragments arranged by size:
4042 1162 546 294 246 159 101 15
13 9 5 3
BseYI C'CCAG_C
Cuts at: 2050 2381 3262 3275 3527 5798 6294 2050
Size: 331 881 13 252 2271 496 2351
Fragments arranged by size:
2351 2271 881 496 331 252 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2306 2927 3629 1163
Size: 573 570 621 702 4129
Fragments arranged by size:
4129 702 621 573 570
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2020 4045 4806 5834 1540
Size: 275 186 19 2025 761 1028 2301
Fragments arranged by size:
2301 2025 1028 761 275 186 19
BsiHKAI G_wGCw'C
Cuts at: 2721 2771 2876 3261 3557 4656 4741 5812 2721
Size: 50 105 385 296 1099 85 1071 3504
Fragments arranged by size:
3504 1099 1071 385 296 105 85 50
BsmI GAATG_Cn'
Cuts at: 4106 4199 4106
Size: 93 6502
BsmBI CGTCTCn'nnnn_
Cuts at: 2597 2990 3360 3873 2597
Size: 393 370 513 5319
Fragments arranged by size:
5319 513 393 370
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2689 2721 2771 2876 3261 3330 3557
Size: 399 899 32 50 105 385 69 227
Cuts at: 3557 3567 3581 3652 3914 4656 4741 5812 1391
Size: 10 14 71 262 742 85 1071 2174
Fragments arranged by size:
2174 1071 899 742 399 385 262 227
105 85 71 69 50 32 14 10
BspEI T'CCGG_A
Cuts at: 6258 6258
Size: 6595
BspHI T'CATG_A
Cuts at: 4484 6214 4484
Size: 1730 4865
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3950 844
Size: 3106 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3019 3342 3641 4482 1394
Size: 429 1196 323 299 841 3507
Fragments arranged by size:
3507 1196 841 429 323 299
BsrDI GCAATG_nn'
Cuts at: 65 1248 2276 3858 5090 5264 65
Size: 1183 1028 1582 1232 174 1396
Fragments arranged by size:
1582 1396 1232 1183 1028 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2355 3680 5239 5426 1700
Size: 110 545 1325 1559 187 2869
Fragments arranged by size:
2869 1559 1325 545 187 110
BsrGI T'GTAC_A
Cuts at: 96 3405 96
Size: 3309 3286
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6595
BssSI C'ACGA_G
Cuts at: 2955 2978 4655 5667 2955
Size: 23 1677 1012 3883
Fragments arranged by size:
3883 1677 1012 23
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3043 3073 3288 794
Size: 2249 30 215 4101
Fragments arranged by size:
4101 2249 215 30
BstBI TT'CG_AA
Cuts at: 5399 5399
Size: 6595
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6595
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6595
BstYI r'GATC_y
Cuts at: 1972 2377 2395 2463 3925 4292 4677 4694 6146
Size: 405 18 68 1462 367 385 17 1452
Cuts at: 6146 6232 6244 6590 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1462 1452 405 385 367 346 86
68 18 17 12
Bsu36I CC'TnA_GG
Cuts at: 3790 3790
Size: 6595
BtgI C'CryG_G
Cuts at: 513 1065 2386 2562 3332 3372 3594 513
Size: 552 1321 176 770 40 222 3514
Fragments arranged by size:
3514 1321 770 552 222 176 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3568 487
Size: 683 56 2342 3514
Fragments arranged by size:
3514 2342 683 56
BtsI GCAGTG_nn'
Cuts at: 2489 2689 3286 4111 4905 4925 2489
Size: 200 597 825 794 20 4159
Fragments arranged by size:
4159 825 794 597 200 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6595
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3786 3821 529
Size: 35 3222 35 3303
Fragments arranged by size:
3303 3222 35 35
DraI TTT'AAA
Cuts at: 3899 3899
Size: 6595
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3854 5602 809
Size: 507 2538 1748 1802
Fragments arranged by size:
2538 1802 1748 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2017 3302 3375
Size: 54 1425 50 275 18 187 1285 73
Cuts at: 3375 4042 4931 6560 8
Size: 667 889 1629 43
Fragments arranged by size:
1629 1425 1285 889 667 275 187 73
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2017 4042 1537
Size: 275 205 2025 4090
Fragments arranged by size:
4090 2025 275 205
EarI CTCTTCn'nnn_
Cuts at: 1353 2165 2285 2541 2721 3009 3210 3358 3777
Size: 812 120 256 180 288 201 148 419
Cuts at: 3777 4525 1353
Size: 748 3423
Fragments arranged by size:
3423 812 748 419 288 256 201 180
148 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5182 5556 5702 387
Size: 1064 138 3593 374 146 1280
Fragments arranged by size:
3593 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2719 2874 3259 3555 2719
Size: 155 385 296 5759
Fragments arranged by size:
5759 385 296 155
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2146 2351 2474 2489 2501 2579 2762
Size: 393 514 205 123 15 12 78 183
Cuts at: 2762 2867 3029 3113 3125 3440 3711 4658 5255
Size: 105 162 84 12 315 271 947 597
Cuts at: 5255 6042 6310 1239
Size: 787 268 1524
Fragments arranged by size:
1524 947 787 597 514 393 315 271
268 205 183 162 123 105 84 78
15 12 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2333 2744 3095 2333
Size: 411 351 5833
Fragments arranged by size:
5833 411 351
EcoO109I rG'GnC_Cy
Cuts at: 2083 2749 2083
Size: 666 5929
EcoRI G'AATT_C
Cuts at: 3904 4282 3904
Size: 378 6217
FspI TGC'GCA
Cuts at: 5101 5101
Size: 6595
HaeII r_GCGC'y
Cuts at: 1678 2191 2329 2661 5742 1678
Size: 513 138 332 3081 2531
Fragments arranged by size:
3081 2531 513 332 138
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2164 2196 3539 3571 3734
Size: 32 135 32 592 32 1343 32 163
Cuts at: 3734 3766 3818 3850 5236 5268 5310 5342 1373
Size: 32 52 32 1386 32 42 32 2626
Fragments arranged by size:
2626 1386 1343 592 163 135 52 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3832 3939 4185 669
Size: 988 281 1894 107 246 3079
Fragments arranged by size:
3079 1894 988 281 246 107
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6595
HpaI GTT'AAC
Cuts at: 4185 4185
Size: 6595
KasI G'GCGC_C
Cuts at: 2325 2657 2325
Size: 332 6263
KpnI G_GTAC'C
Cuts at: 3920 3920
Size: 6595
MfeI C'AATT_G
Cuts at: 4194 4194
Size: 6595
MluI A'CGCG_T
Cuts at: 6471 6471
Size: 6595
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3870 5709 5893 1305
Size: 2565 1839 184 2007
Fragments arranged by size:
2565 2007 1839 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4773
Fragments arranged by size:
4773 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4553 4912 5071 518
Size: 648 3387 359 159 2042
Fragments arranged by size:
3387 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2346 2388 3040 3334 3374 4011 4688 5836
Size: 740 42 652 294 40 637 677 1148
Cuts at: 5836 6081 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 740 677 652 637 294 245
42 40
NarI GG'CG_CC
Cuts at: 2326 2658 2326
Size: 332 6263
NcoI C'CATG_G
Cuts at: 513 1065 2562 513
Size: 552 1497 4546
Fragments arranged by size:
4546 1497 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6595
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6595
NotI GC'GGCC_GC
Cuts at: 4042 4042
Size: 6595
NspI r_CATG'y
Cuts at: 1132 1229 2114 3953 5498 1132
Size: 97 885 1839 1545 2229
Fragments arranged by size:
2229 1839 1545 885 97
PasI CC'CwG_GG
Cuts at: 1348 2213 2638 1348
Size: 865 425 5305
Fragments arranged by size:
5305 865 425
PciI A'CATG_T
Cuts at: 5494 5494
Size: 6595
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6595
PfoI T'CCnGG_A
Cuts at: 2373 2702 2712 2373
Size: 329 10 6256
Fragments arranged by size:
6256 329 10
PmeI GTTT'AAAC
Cuts at: 3899 3899
Size: 6595
PmlI CAC'GTG
Cuts at: 2284 2284
Size: 6595
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4664 4696 6177 6209 4664
Size: 32 1481 32 5050
Fragments arranged by size:
5050 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2749 2749
Size: 6595
PshAI GACnn'nnGTC
Cuts at: 1613 2123 2222 1613
Size: 510 99 5986
Fragments arranged by size:
5986 510 99
PsiI TTA'TAA
Cuts at: 4165 4165
Size: 6595
PstI C_TGCA'G
Cuts at: 830 3033 3048 3078 3390 3947 830
Size: 2203 15 30 312 557 3478
Fragments arranged by size:
3478 2203 557 312 30 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6595
PvuII CAG'CTG
Cuts at: 3040 3040
Size: 6595
RsrII CG'GwC_CG
Cuts at: 3021 3021
Size: 6595
SacI G_AGCT'C
Cuts at: 2721 2876 3261 3557 2721
Size: 155 385 296 5759
Fragments arranged by size:
5759 385 296 155
SacII CC_GC'GG
Cuts at: 2389 3335 3375 2389
Size: 946 40 5609
Fragments arranged by size:
5609 946 40
SalI G'TCGA_C
Cuts at: 1936 3937 1936
Size: 2001 4594
SapI GCTCTTCn'nnn_
Cuts at: 2285 3009 3777 2285
Size: 724 768 5103
Fragments arranged by size:
5103 768 724
SbfI CC_TGCA'GG
Cuts at: 3078 3390 3947 3078
Size: 312 557 5726
Fragments arranged by size:
5726 557 312
ScaI AGT'ACT
Cuts at: 4843 6555 4843
Size: 1712 4883
SfcI C'TryA_G
Cuts at: 826 1045 2227 3029 3044 3074 3386 3943 5078
Size: 219 1182 802 15 30 312 557 1135
Cuts at: 5078 5759 5950 826
Size: 681 191 1471
Fragments arranged by size:
1471 1182 1135 802 681 557 312 219
191 30 15
SfoI GGC'GCC
Cuts at: 2327 2659 2327
Size: 332 6263
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6595
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6595
SmaI CCC'GGG
Cuts at: 1872 3662 3922 1872
Size: 1790 260 4545
Fragments arranged by size:
4545 1790 260
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4699 5600 5862 820
Size: 197 926 2756 901 262 1553
Fragments arranged by size:
2756 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4386 493
Size: 3893 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6595
SphI G_CATG'C
Cuts at: 1132 2114 3953 1132
Size: 982 1839 3774
Fragments arranged by size:
3774 1839 982
SrfI GCCC'GGGC
Cuts at: 3662 3662
Size: 6595
SspI AAT'ATT
Cuts at: 5 52 4519 5
Size: 47 4467 2081
Fragments arranged by size:
4467 2081 47
StuI AGG'CCT
Cuts at: 1517 3540 3803 1517
Size: 2023 263 4309
Fragments arranged by size:
4309 2023 263
StyI C'CwwG_G
Cuts at: 513 1065 2562 3804 4034 513
Size: 552 1497 1242 230 3074
Fragments arranged by size:
3074 1497 1242 552 230
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2537 3541 4622 4639 4792 4977 5422
Size: 1255 957 1004 1081 17 153 185 445
Cuts at: 5422 325
Size: 1498
Fragments arranged by size:
1498 1255 1081 1004 957 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3405 4841 6553
Size: 274 80 33 51 1443 1428 1436 1712
Cuts at: 6553 96
Size: 138
Fragments arranged by size:
1712 1443 1436 1428 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2099 2131 2468 2500 3092 3124 6555 6587 2099
Size: 32 337 32 592 32 3431 32 2107
Fragments arranged by size:
3431 2107 592 337 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6595
XbaI T'CTAG_A
Cuts at: 3931 3931
Size: 6595
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6595
XmaI C'CCGG_G
Cuts at: 1870 3660 3920 1870
Size: 1790 260 4545
Fragments arranged by size:
4545 1790 260
XmnI GAAnn'nnTTC
Cuts at: 4724 4724
Size: 6595
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3829 4403 276
Size: 53 83 186 936 2295 574 2468
Fragments arranged by size:
2468 2295 936 574 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI
SanDI SexAI SfiI SwaI XcmI