Restriction map for
pFN21AA1018
(Circular) (Six-base) MAPSORT of: pFN21AA1018.seq Check: 7526 from: 1 to: 7756
pFN21AA1018 7756 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2128 2128
Size: 7756
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5566 278
Size: 53 83 186 936 4030 2468
Fragments arranged by size:
4030 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2834 5099 1937
Size: 897 2265 4594
Fragments arranged by size:
4594 2265 897
Acc65I G'GTAC_C
Cuts at: 5077 5077
Size: 7756
AclI AA'CG_TT
Cuts at: 1563 5883 6256 1563
Size: 4320 373 3063
Fragments arranged by size:
4320 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2577 2823 2916 3423 4501 4564 4763 5022
Size: 945 246 93 507 1078 63 199 259
Cuts at: 5022 5819 7203 7471 1632
Size: 797 1384 268 1917
Fragments arranged by size:
1917 1384 1078 945 797 507 268 259
246 199 93 63
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7559
AflIII A'CryG_T
Cuts at: 1203 2646 4047 4339 6655 7632 1203
Size: 1443 1401 292 2316 977 1327
Fragments arranged by size:
2316 1443 1401 1327 977 292
AgeI A'CCGG_T
Cuts at: 3113 6587 3113
Size: 3474 4282
AhdI GACnn_n'nnGTC
Cuts at: 6485 6485
Size: 7756
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7756
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4863 1184
Size: 3679 4077
AlwNI CAG_nnn'CTG
Cuts at: 3242 3383 3490 4993 5499 7071 3242
Size: 141 107 1503 506 1572 3927
Fragments arranged by size:
3927 1572 1503 506 141 107
ApaI G_GGCC'C
Cuts at: 4817 4817
Size: 7756
ApaLI G'TGCA_C
Cuts at: 4615 5813 6969 4615
Size: 1198 1156 5402
Fragments arranged by size:
5402 1198 1156
ApoI r'AATT_y
Cuts at: 1493 2245 2326 2528 2616 3221 4095 5065 5291
Size: 752 81 202 88 605 874 970 226
Cuts at: 5291 5443 7552 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 970 874 752 605 226 202
152 88 81
AseI AT'TA_AT
Cuts at: 160 1032 6310 160
Size: 872 5278 1606
Fragments arranged by size:
5278 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7756
AvaI C'yCGr_G
Cuts at: 1870 1943 2739 4685 5081 1870
Size: 73 796 1946 396 4545
Fragments arranged by size:
4545 1946 796 396 73
BamHI G'GATC_C
Cuts at: 2221 4207 5086 5453 2221
Size: 1986 879 367 4524
Fragments arranged by size:
4524 1986 879 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2155 2178 4839 5077 6532
Size: 325 217 627 368 23 2661 238 1455
Cuts at: 6532 618
Size: 1842
Fragments arranged by size:
2661 1842 1455 627 368 325 238 217
23
BanII G_rGCy'C
Cuts at: 1391 3894 4817 5075 1391
Size: 2503 923 258 4072
Fragments arranged by size:
4072 2503 923 258
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2850 3693 4570 928
Size: 971 951 843 877 4114
Fragments arranged by size:
4114 971 951 877 843
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5947 5981 1903
Size: 34 4010 34 3678
Fragments arranged by size:
4010 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5648 6858 5648
Size: 1210 6546
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7756
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4412 4595 4655
Size: 107 122 71 631 605 2740 183 60
Cuts at: 4655 4709 4812 6367 136
Size: 54 103 1555 1525
Fragments arranged by size:
2740 1555 1525 631 605 183 122 107
103 71 60 54
BglII A'GATC_T
Cuts at: 7751 7751
Size: 7756
BlpI GC'TnA_GC
Cuts at: 5173 5173
Size: 7756
Bme1580I G_kGCm'C
Cuts at: 1790 4367 4619 4817 5817 6973 1790
Size: 2577 252 198 1000 1156 2573
Fragments arranged by size:
2577 2573 1156 1000 252 198
BmgBI CAC'GTC
Cuts at: 1318 4773 1318
Size: 3455 4301
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3883 5487 6445
Size: 416 53 83 399 24 2466 1604 958
Cuts at: 6445 442
Size: 1753
Fragments arranged by size:
2466 1753 1604 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7756
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4892 4892
Size: 7756
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 6416 1239
Size: 5177 2579
Bpu10I CC'TnA_GC
Cuts at: 3885 4788 5051 3885
Size: 903 263 6590
Fragments arranged by size:
6590 903 263
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3185 4673 5881 6746 7044 3185
Size: 1488 1208 865 298 3897
Fragments arranged by size:
3897 1488 1208 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6419 882
Size: 617 4920 2219
Fragments arranged by size:
4920 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2494 4342 4719 5547 493
Size: 711 1290 1848 377 828 2702
Fragments arranged by size:
2702 1848 1290 828 711 377
BsaBI GATnn'nnATC
Cuts at: 7750 7750
Size: 7756
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3498 3690 4642 5563
Size: 53 83 186 936 1965 192 952 921
Cuts at: 5563 5945 6580 275
Size: 382 635 1451
Fragments arranged by size:
1965 1451 952 936 921 635 382 192
186 83 53
BsaWI w'CCGG_w
Cuts at: 3113 4210 4570 6189 6587 6861 7008 7419 3113
Size: 1097 360 1619 398 274 147 411 3450
Fragments arranged by size:
3450 1619 1097 411 398 360 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3068 3098 1178
Size: 30 165 30 1665 30 5836
Fragments arranged by size:
5836 1665 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3012 4463 4526 4703 1186
Size: 1826 1451 63 177 4239
Fragments arranged by size:
4239 1826 1451 177 63
BseYI C'CCAG_C
Cuts at: 4645 4758 4993 6959 7455 4645
Size: 113 235 1966 496 4946
Fragments arranged by size:
4946 1966 496 235 113
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3517 3585 4855 1163
Size: 573 1781 68 1270 4064
Fragments arranged by size:
4064 1781 1270 573 68
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3745 5206 5967 6995 1540
Size: 275 186 1744 1461 761 1028 2301
Fragments arranged by size:
2301 1744 1461 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3894 4442 4619 4824 5817 5902 6973 3894
Size: 548 177 205 993 85 1071 4677
Fragments arranged by size:
4677 1071 993 548 205 177 85
BsmI GAATG_Cn'
Cuts at: 2679 3066 3364 4598 5267 5360 2679
Size: 387 298 1234 669 93 5075
Fragments arranged by size:
5075 1234 669 387 298 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4779 4779
Size: 7756
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3894 4367 4442 4619 4817 4824 5075
Size: 399 2104 473 75 177 198 7 251
Cuts at: 5075 5817 5902 6973 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 2104 1071 742 473 399 251 198
177 85 75 7
BspEI T'CCGG_A
Cuts at: 4210 4570 7419 4210
Size: 360 2849 4547
Fragments arranged by size:
4547 2849 360
BspHI T'CATG_A
Cuts at: 4675 5645 7375 4675
Size: 970 1730 5056
Fragments arranged by size:
5056 1730 970
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2128 2221 5111 844
Size: 1284 93 2890 3489
Fragments arranged by size:
3489 2890 1284 93
BsrBI CCG'CTC
Cuts at: 1394 1823 5643 1394
Size: 429 3820 3507
Fragments arranged by size:
3820 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3100 3864 6251 6425 65
Size: 1183 1852 764 2387 174 1396
Fragments arranged by size:
2387 1852 1396 1183 764 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2175 2534 3113 6400 6587 1700
Size: 110 365 359 579 3287 187 2869
Fragments arranged by size:
3287 2869 579 365 359 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7756
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7756
BssSI C'ACGA_G
Cuts at: 5816 6828 5816
Size: 1012 6744
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2120 2990 4362 794
Size: 1326 870 1372 4188
Fragments arranged by size:
4188 1372 1326 870
BstBI TT'CG_AA
Cuts at: 6560 6560
Size: 7756
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7756
BstXI CCAn_nnnn'nTGG
Cuts at: 3592 3592
Size: 7756
BstYI r'GATC_y
Cuts at: 1972 2221 4207 4792 5086 5453 5838 5855 7307
Size: 249 1986 585 294 367 385 17 1452
Cuts at: 7307 7393 7405 7751 1972
Size: 86 12 346 1977
Fragments arranged by size:
1986 1977 1452 585 385 367 346 294
249 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 4428 513
Size: 552 3363 3841
Fragments arranged by size:
3841 3363 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7017
Fragments arranged by size:
7017 683 56
BtsI GCAGTG_nn'
Cuts at: 3573 5272 6066 6086 3573
Size: 1699 794 20 5243
Fragments arranged by size:
5243 1699 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7756
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7721
DraI TTT'AAA
Cuts at: 2392 2653 3576 5060 2392
Size: 261 923 1484 5088
Fragments arranged by size:
5088 1484 923 261
DraIII CAC_nnn'GTG
Cuts at: 4373 4373
Size: 7756
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6763 809
Size: 507 5447 1802
Fragments arranged by size:
5447 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2338 3513 3742
Size: 54 1425 50 275 18 508 1175 229
Cuts at: 3742 3861 5203 6092 7721 8
Size: 119 1342 889 1629 43
Fragments arranged by size:
1629 1425 1342 1175 889 508 275 229
119 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3742 5203 1537
Size: 275 1930 1461 4090
Fragments arranged by size:
4090 1930 1461 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2596 2667 3309 3974 4304 4782 5686 1353
Size: 1243 71 642 665 330 478 904 3423
Fragments arranged by size:
3423 1243 904 665 642 478 330 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4220 6343 6717 6863 387
Size: 1064 138 2631 2123 374 146 1280
Fragments arranged by size:
2631 2123 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3892 3892
Size: 7756
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2577 2823 2916 3423 4501 4564 4763
Size: 393 945 246 93 507 1078 63 199
Cuts at: 4763 5022 5819 6416 7203 7471 1239
Size: 259 797 597 787 268 1524
Fragments arranged by size:
1524 1078 945 797 787 597 507 393
268 259 246 199 93 63
EcoO109I rG'GnC_Cy
Cuts at: 3623 4813 4814 4873 4937 3623
Size: 1190 1 59 64 6442
Fragments arranged by size:
6442 1190 64 59 1
EcoRI G'AATT_C
Cuts at: 5065 5443 5065
Size: 378 7378
EcoRV GAT'ATC
Cuts at: 3055 3055
Size: 7756
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2867 4315 2867
Size: 1448 6308
FspI TGC'GCA
Cuts at: 6262 6262
Size: 7756
HaeII r_GCGC'y
Cuts at: 1678 4168 6903 1678
Size: 2490 2735 2531
Fragments arranged by size:
2735 2531 2490
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2954 2986 3068 3100 4000
Size: 32 135 32 1382 32 82 32 900
Cuts at: 4000 4032 4784 4816 6397 6429 6471 6503 1373
Size: 32 752 32 1581 32 42 32 2626
Fragments arranged by size:
2626 1581 1382 900 752 135 82 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3666 3736 5100 5346 669
Size: 988 281 1728 70 1364 246 3079
Fragments arranged by size:
3079 1728 1364 988 281 246 70
HindIII A'AGCT_T
Cuts at: 748 2050 748
Size: 1302 6454
HpaI GTT'AAC
Cuts at: 5346 5346
Size: 7756
KpnI G_GTAC'C
Cuts at: 5081 5081
Size: 7756
MfeI C'AATT_G
Cuts at: 5355 5355
Size: 7756
MluI A'CGCG_T
Cuts at: 7632 7632
Size: 7756
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5011 6870 7054 1305
Size: 3706 1859 184 2007
Fragments arranged by size:
3706 2007 1859 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2340 3515 3863 10
Size: 54 1425 343 508 1175 348 3903
Fragments arranged by size:
3903 1425 1175 508 348 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2184 3833 4050 4556 5714 6073 6232
Size: 648 1018 1649 217 506 1158 359 159
Cuts at: 6232 518
Size: 2042
Fragments arranged by size:
2042 1649 1158 1018 648 506 359 217
159
MspA1I CmG'CkG
Cuts at: 1606 2950 3490 4418 4479 4669 4758 5172 5849
Size: 1344 540 928 61 190 89 414 677
Cuts at: 5849 6997 7242 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1344 1148 928 677 540 414 245
190 89 61
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7204
NdeI CA'TA_TG
Cuts at: 387 2691 387
Size: 2304 5452
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7756
NotI GC'GGCC_GC
Cuts at: 5203 5203
Size: 7756
NspI r_CATG'y
Cuts at: 1132 1229 2901 3838 4051 5114 6659 1132
Size: 97 1672 937 213 1063 1545 2229
Fragments arranged by size:
2229 1672 1545 1063 937 213 97
PasI CC'CwG_GG
Cuts at: 1348 3627 1348
Size: 2279 5477
PciI A'CATG_T
Cuts at: 4047 6655 4047
Size: 2608 5148
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4373 1108
Size: 3265 4491
PmeI GTTT'AAAC
Cuts at: 5060 5060
Size: 7756
PmlI CAC'GTG
Cuts at: 4342 4719 4342
Size: 377 7379
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5825 5857 7338 7370 5825
Size: 32 1481 32 6211
Fragments arranged by size:
6211 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7756
PsiI TTA'TAA
Cuts at: 5326 5326
Size: 7756
PspOMI G'GGCC_C
Cuts at: 4813 4813
Size: 7756
PstI C_TGCA'G
Cuts at: 830 2986 3030 4038 4668 4989 5108 830
Size: 2156 44 1008 630 321 119 3478
Fragments arranged by size:
3478 2156 1008 630 321 119 44
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7756
PvuII CAG'CTG
Cuts at: 2950 3490 4669 4758 2950
Size: 540 1179 89 5948
Fragments arranged by size:
5948 1179 540 89
SacI G_AGCT'C
Cuts at: 3894 3894
Size: 7756
SalI G'TCGA_C
Cuts at: 1936 5098 1936
Size: 3162 4594
SbfI CC_TGCA'GG
Cuts at: 5108 5108
Size: 7756
ScaI AGT'ACT
Cuts at: 6004 7716 6004
Size: 1712 6044
SexAI A'CCwGG_T
Cuts at: 4884 4884
Size: 7756
SfcI C'TryA_G
Cuts at: 826 1045 2982 3026 3371 4034 4664 4985 5104
Size: 219 1937 44 345 663 630 321 119
Cuts at: 5104 6239 6920 7111 826
Size: 1135 681 191 1471
Fragments arranged by size:
1937 1471 1135 681 663 630 345 321
219 191 119 44
SfiI GGCCn_nnn'nGGCC
Cuts at: 4412 4412
Size: 7756
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7756
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7756
SmaI CCC'GGG
Cuts at: 1872 2741 5083 1872
Size: 869 2342 4545
Fragments arranged by size:
4545 2342 869
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3164 4652 5860 6761 7023 820
Size: 197 926 1221 1488 1208 901 262 1553
Fragments arranged by size:
1553 1488 1221 1208 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2494 5547 493
Size: 2001 3053 2702
Fragments arranged by size:
3053 2702 2001
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7756
SphI G_CATG'C
Cuts at: 1132 5114 1132
Size: 3982 3774
SspI AAT'ATT
Cuts at: 5 52 5680 5
Size: 47 5628 2081
Fragments arranged by size:
5628 2081 47
StuI AGG'CCT
Cuts at: 1517 2041 1517
Size: 524 7232
StyI C'CwwG_G
Cuts at: 513 1065 3591 5195 513
Size: 552 2526 1604 3074
Fragments arranged by size:
3074 2526 1604 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3683 3981 4696 5783 5800 5953 6138
Size: 1255 2103 298 715 1087 17 153 185
Cuts at: 6138 6583 325
Size: 445 1498
Fragments arranged by size:
2103 1498 1255 1087 715 445 298 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6002 7714 96
Size: 274 80 33 51 1443 4025 1712 138
Fragments arranged by size:
4025 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7716 7748 7716
Size: 32 7724
Tth111I GACn'n_nGTC
Cuts at: 1316 4863 1316
Size: 3547 4209
XbaI T'CTAG_A
Cuts at: 5092 5092
Size: 7756
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4496 4496
Size: 7756
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7756
XmaI C'CCGG_G
Cuts at: 1870 2739 5081 1870
Size: 869 2342 4545
Fragments arranged by size:
4545 2342 869
XmnI GAAnn'nnTTC
Cuts at: 5885 5885
Size: 7756
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5564 276
Size: 53 83 186 936 4030 2468
Fragments arranged by size:
4030 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BstZ17I Bsu36I EcoNI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspXI
PsrI RsrII SacII SanDI SapI SfoI SrfI SwaI