Restriction map for
pFN21AA1016
(Circular) (Six-base) MAPSORT of: pFN21AA1016.seq Check: 4743 from: 1 to: 6889
pFN21AA1016 6889 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4699 278
Size: 53 83 186 936 3163 2468
Fragments arranged by size:
3163 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3796 4232 1937
Size: 1859 436 4594
Fragments arranged by size:
4594 1859 436
Acc65I G'GTAC_C
Cuts at: 4210 4210
Size: 6889
AclI AA'CG_TT
Cuts at: 1563 5016 5389 1563
Size: 3453 373 3063
Fragments arranged by size:
3453 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3665 4952 6336 6604 1632
Size: 2033 1287 1384 268 1917
Fragments arranged by size:
2033 1917 1384 1287 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6692
AflIII A'CryG_T
Cuts at: 1203 5788 6765 1203
Size: 4585 977 1327
Fragments arranged by size:
4585 1327 977
AgeI A'CCGG_T
Cuts at: 5720 5720
Size: 6889
AhdI GACnn_n'nnGTC
Cuts at: 5618 5618
Size: 6889
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6889
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2753 2845 1184
Size: 1569 92 5228
Fragments arranged by size:
5228 1569 92
AlwNI CAG_nnn'CTG
Cuts at: 2437 3986 4632 6204 2437
Size: 1549 646 1572 3122
Fragments arranged by size:
3122 1572 1549 646
ApaLI G'TGCA_C
Cuts at: 3296 4946 6102 3296
Size: 1650 1156 4083
Fragments arranged by size:
4083 1650 1156
ApoI r'AATT_y
Cuts at: 1493 2258 2373 3128 3140 3233 3679 4198 4424
Size: 765 115 755 12 93 446 519 226
Cuts at: 4424 4576 6685 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 765 755 519 446 226 152
115 93 12
AseI AT'TA_AT
Cuts at: 160 1032 5443 160
Size: 872 4411 1606
Fragments arranged by size:
4411 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6889
AvaI C'yCGr_G
Cuts at: 1870 1943 2279 4064 4214 1870
Size: 73 336 1785 150 4545
Fragments arranged by size:
4545 1785 336 150 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2380 2413 2380
Size: 33 6856
BamHI G'GATC_C
Cuts at: 4219 4586 4219
Size: 367 6522
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2123 4210 5665 618
Size: 325 217 627 336 2087 1455 1842
Fragments arranged by size:
2087 1842 1455 627 336 325 217
BanII G_rGCy'C
Cuts at: 1391 4208 1391
Size: 2817 4072
BbeI G_GCGC'C
Cuts at: 2127 2127
Size: 6889
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3036 3267 3810 928
Size: 971 1137 231 543 4007
Fragments arranged by size:
4007 1137 971 543 231
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5080 5114 1903
Size: 34 3143 34 3678
Fragments arranged by size:
3678 3143 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4781 5991 4781
Size: 1210 5679
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6889
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5500 136
Size: 107 122 71 631 605 3828 1525
Fragments arranged by size:
3828 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2698 6884 2698
Size: 4186 2703
BlpI GC'TnA_GC
Cuts at: 4306 4306
Size: 6889
Bme1580I G_kGCm'C
Cuts at: 1790 3300 4950 6106 1790
Size: 1510 1650 1156 2573
Fragments arranged by size:
2573 1650 1510 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6889
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4620 5578 442
Size: 416 53 83 399 24 3203 958 1753
Fragments arranged by size:
3203 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6889
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2213 2802 3530 4087 5549 1239
Size: 974 589 728 557 1462 2579
Fragments arranged by size:
2579 1462 974 728 589 557
Bpu10I CC'TnA_GC
Cuts at: 2237 3110 2237
Size: 873 6016
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2225 2473 2813 5014 5879 6177 2225
Size: 248 340 2201 865 298 2937
Fragments arranged by size:
2937 2201 865 340 298 248
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3189 3227 5552 882
Size: 617 1690 38 2325 2219
Fragments arranged by size:
2325 2219 1690 617 38
BsaAI yAC'GTr
Cuts at: 493 1204 2944 4680 493
Size: 711 1740 1736 2702
Fragments arranged by size:
2702 1740 1736 711
BsaBI GATnn'nnATC
Cuts at: 6883 6883
Size: 6889
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 2124 4696 5078
Size: 53 83 186 936 479 112 2572 382
Cuts at: 5078 5713 275
Size: 635 1451
Fragments arranged by size:
2572 1451 936 635 479 382 186 112
83 53
BsaWI w'CCGG_w
Cuts at: 3148 3520 3711 5322 5720 5994 6141 6552 3148
Size: 372 191 1611 398 274 147 411 3485
Fragments arranged by size:
3485 1611 411 398 372 274 191 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3083 3113 1178
Size: 30 165 30 1680 30 4954
Fragments arranged by size:
4954 1680 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2222 2805 1186
Size: 1036 583 5270
Fragments arranged by size:
5270 1036 583
BseYI C'CCAG_C
Cuts at: 2616 6092 6588 2616
Size: 3476 496 2917
Fragments arranged by size:
3476 2917 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2048 2324 3471 1163
Size: 573 312 276 1147 4581
Fragments arranged by size:
4581 1147 573 312 276
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3872 4339 5100 6128 1540
Size: 275 186 1871 467 761 1028 2301
Fragments arranged by size:
2301 1871 1028 761 467 275 186
BsiHKAI G_wGCw'C
Cuts at: 2742 3300 4950 5035 6106 2742
Size: 558 1650 85 1071 3525
Fragments arranged by size:
3525 1650 1071 558 85
BsmI GAATG_Cn'
Cuts at: 4180 4400 4493 4180
Size: 220 93 6576
Fragments arranged by size:
6576 220 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2742 3300 4208 4950 5035 6106 1391
Size: 399 952 558 908 742 85 1071 2174
Fragments arranged by size:
2174 1071 952 908 742 558 399 85
BspEI T'CCGG_A
Cuts at: 3711 6552 3711
Size: 2841 4048
BspHI T'CATG_A
Cuts at: 4778 6508 4778
Size: 1730 5159
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2181 4244 844
Size: 1337 2063 3489
Fragments arranged by size:
3489 2063 1337
BsrBI CCG'CTC
Cuts at: 1394 1823 4101 4776 1394
Size: 429 2278 675 3507
Fragments arranged by size:
3507 2278 675 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3360 5384 5558 65
Size: 1183 2112 2024 174 1396
Fragments arranged by size:
2112 2024 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2120 5533 5720 1700
Size: 110 310 3413 187 2869
Fragments arranged by size:
3413 2869 310 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6889
BssHII G'CGCG_C
Cuts at: 1925 2197 2199 1925
Size: 272 2 6615
Fragments arranged by size:
6615 272 2
BssSI C'ACGA_G
Cuts at: 2741 3097 4949 5961 2741
Size: 356 1852 1012 3669
Fragments arranged by size:
3669 1852 1012 356
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6889
BstBI TT'CG_AA
Cuts at: 4036 5693 4036
Size: 1657 5232
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6889
BstYI r'GATC_y
Cuts at: 1972 2698 3394 4219 4586 4971 4988 6440 6526
Size: 726 696 825 367 385 17 1452 86
Cuts at: 6526 6538 6884 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 825 726 696 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2412 2412
Size: 6889
BtgI C'CryG_G
Cuts at: 513 1065 2004 2110 2916 3866 513
Size: 552 939 106 806 950 3536
Fragments arranged by size:
3536 950 939 806 552 106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3089 487
Size: 683 56 1863 4287
Fragments arranged by size:
4287 1863 683 56
BtsI GCAGTG_nn'
Cuts at: 4405 5199 5219 4405
Size: 794 20 6075
Fragments arranged by size:
6075 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6889
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6854
DraI TTT'AAA
Cuts at: 3438 4193 3438
Size: 755 6134
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5896 809
Size: 507 4580 1802
Fragments arranged by size:
4580 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2214 3921 4336
Size: 54 1425 50 275 18 384 1707 415
Cuts at: 4336 5225 6854 8
Size: 889 1629 43
Fragments arranged by size:
1707 1629 1425 889 415 384 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4336 1537
Size: 275 2524 4090
Fragments arranged by size:
4090 2524 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2544 3264 3282 3732 4819 1353
Size: 1191 720 18 450 1087 3423
Fragments arranged by size:
3423 1191 1087 720 450 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2461 3851 5476 5850 5996 387
Size: 1064 138 872 1390 1625 374 146 1280
Fragments arranged by size:
1625 1390 1280 1064 872 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2213 2802 3530 3665 4087 4952 5549
Size: 393 581 589 728 135 422 865 597
Cuts at: 5549 6336 6604 1239
Size: 787 268 1524
Fragments arranged by size:
1524 865 787 728 597 589 581 422
393 268 135
EcoO109I rG'GnC_Cy
Cuts at: 4108 4108
Size: 6889
EcoRI G'AATT_C
Cuts at: 4198 4576 4198
Size: 378 6511
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2737 2737
Size: 6889
FspI TGC'GCA
Cuts at: 5395 5395
Size: 6889
HaeII r_GCGC'y
Cuts at: 1678 2127 2244 6036 1678
Size: 449 117 3792 2531
Fragments arranged by size:
3792 2531 449 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2446 2478 3774 3806 3980
Size: 32 135 32 874 32 1296 32 174
Cuts at: 3980 4012 5530 5562 5604 5636 1373
Size: 32 1518 32 42 32 2626
Fragments arranged by size:
2626 1518 1296 874 174 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3853 4048 4233 4479 669
Size: 988 281 1915 195 185 246 3079
Fragments arranged by size:
3079 1915 988 281 246 195 185
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6889
HpaI GTT'AAC
Cuts at: 4479 4479
Size: 6889
KasI G'GCGC_C
Cuts at: 2123 2123
Size: 6889
KpnI G_GTAC'C
Cuts at: 4214 4214
Size: 6889
MfeI C'AATT_G
Cuts at: 4488 4488
Size: 6889
MluI A'CGCG_T
Cuts at: 6765 6765
Size: 6889
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2959 3999 6003 6187 1305
Size: 1654 1040 2004 184 2007
Fragments arranged by size:
2007 2004 1654 1040 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3923 10
Size: 54 1425 343 2091 2976
Fragments arranged by size:
2976 2091 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3776 4173 4847 5206 5365 518
Size: 648 2610 397 674 359 159 2042
Fragments arranged by size:
2610 2042 674 648 397 359 159
MspA1I CmG'CkG
Cuts at: 1606 2160 2467 2592 2776 2894 3367 3508 3902
Size: 554 307 125 184 118 473 141 394
Cuts at: 3902 4305 4982 6130 6375 1606
Size: 403 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 554 473 403 394 307
245 184 141 125 118
NarI GG'CG_CC
Cuts at: 2124 2124
Size: 6889
NcoI C'CATG_G
Cuts at: 513 1065 2004 2916 513
Size: 552 939 912 4486
Fragments arranged by size:
4486 939 912 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6889
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6889
NotI GC'GGCC_GC
Cuts at: 4336 4336
Size: 6889
NspI r_CATG'y
Cuts at: 1132 1229 2880 4247 5792 1132
Size: 97 1651 1367 1545 2229
Fragments arranged by size:
2229 1651 1545 1367 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6889
PciI A'CATG_T
Cuts at: 5788 5788
Size: 6889
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2917 2944 1108
Size: 1809 27 5053
Fragments arranged by size:
5053 1809 27
PmeI GTTT'AAAC
Cuts at: 4193 4193
Size: 6889
PmlI CAC'GTG
Cuts at: 2944 2944
Size: 6889
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4958 4990 6471 6503 4958
Size: 32 1481 32 5344
Fragments arranged by size:
5344 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4108 4108
Size: 6889
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6889
PsiI TTA'TAA
Cuts at: 4459 4459
Size: 6889
PstI C_TGCA'G
Cuts at: 830 2433 2811 3607 4017 4241 830
Size: 1603 378 796 410 224 3478
Fragments arranged by size:
3478 1603 796 410 378 224
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6889
PvuII CAG'CTG
Cuts at: 2467 2592 2776 2894 3367 3508 2467
Size: 125 184 118 473 141 5848
Fragments arranged by size:
5848 473 184 141 125 118
SalI G'TCGA_C
Cuts at: 1936 4231 1936
Size: 2295 4594
SapI GCTCTTCn'nnn_
Cuts at: 3264 3264
Size: 6889
SbfI CC_TGCA'GG
Cuts at: 4241 4241
Size: 6889
ScaI AGT'ACT
Cuts at: 5137 6849 5137
Size: 1712 5177
SfcI C'TryA_G
Cuts at: 826 1045 2429 2807 3603 3690 4013 4237 5372
Size: 219 1384 378 796 87 323 224 1135
Cuts at: 5372 6053 6244 826
Size: 681 191 1471
Fragments arranged by size:
1471 1384 1135 796 681 378 323 224
219 191 87
SfoI GGC'GCC
Cuts at: 2125 2125
Size: 6889
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6889
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6889
SmaI CCC'GGG
Cuts at: 1872 2281 4216 1872
Size: 409 1935 4545
Fragments arranged by size:
4545 1935 409
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2204 2452 2792 4993 5894 6156
Size: 197 926 261 248 340 2201 901 262
Cuts at: 6156 820
Size: 1553
Fragments arranged by size:
2201 1553 926 901 340 262 261 248
197
SnaBI TAC'GTA
Cuts at: 493 4680 493
Size: 4187 2702
SpeI A'CTAG_T
Cuts at: 152 2692 152
Size: 2540 4349
SphI G_CATG'C
Cuts at: 1132 2880 4247 1132
Size: 1748 1367 3774
Fragments arranged by size:
3774 1748 1367
SspI AAT'ATT
Cuts at: 5 52 4813 5
Size: 47 4761 2081
Fragments arranged by size:
4761 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6889
StyI C'CwwG_G
Cuts at: 513 1065 2004 2706 2916 4328 513
Size: 552 939 702 210 1412 3074
Fragments arranged by size:
3074 1412 939 702 552 210
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2082 3155 4916 4933 5086 5271 5716
Size: 1255 502 1073 1761 17 153 185 445
Cuts at: 5716 325
Size: 1498
Fragments arranged by size:
1761 1498 1255 1073 502 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5135 6847 96
Size: 274 80 33 51 1443 3158 1712 138
Fragments arranged by size:
3158 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3083 3115 6849 6881 3083
Size: 32 3734 32 3091
Fragments arranged by size:
3734 3091 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6889
XbaI T'CTAG_A
Cuts at: 4225 4225
Size: 6889
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3916 3916
Size: 6889
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6889
XmaI C'CCGG_G
Cuts at: 1870 2279 4214 1870
Size: 409 1935 4545
Fragments arranged by size:
4545 1935 409
XmnI GAAnn'nnTTC
Cuts at: 3759 5018 3759
Size: 1259 5630
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4697 276
Size: 53 83 186 936 3163 2468
Fragments arranged by size:
3163 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SrfI SwaI