Restriction map for
pFN21AA1012
(Circular) (Six-base) MAPSORT of: pFN21AA1012.seq Check: 8016 from: 1 to: 9010
pFN21AA1012 9010 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6820 278
Size: 53 83 186 936 5284 2468
Fragments arranged by size:
5284 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5010 6353 1937
Size: 3073 1343 4594
Fragments arranged by size:
4594 3073 1343
Acc65I G'GTAC_C
Cuts at: 6331 6331
Size: 9010
AclI AA'CG_TT
Cuts at: 1563 7137 7510 1563
Size: 5574 373 3063
Fragments arranged by size:
5574 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2381 2888 4443 7073 8457 8725 1632
Size: 749 507 1555 2630 1384 268 1917
Fragments arranged by size:
2630 1917 1555 1384 749 507 268
AflII C'TTAA_G
Cuts at: 820 1017 2132 820
Size: 197 1115 7698
Fragments arranged by size:
7698 1115 197
AflIII A'CryG_T
Cuts at: 1203 7909 8886 1203
Size: 6706 977 1327
Fragments arranged by size:
6706 1327 977
AgeI A'CCGG_T
Cuts at: 7841 7841
Size: 9010
AhdI GACnn_n'nnGTC
Cuts at: 7739 7739
Size: 9010
AleI CACnn'nnGTG
Cuts at: 1166 2532 1166
Size: 1366 7644
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3661 1184
Size: 2477 6533
AlwNI CAG_nnn'CTG
Cuts at: 3410 3569 4928 6753 8325 3410
Size: 159 1359 1825 1572 4095
Fragments arranged by size:
4095 1825 1572 1359 159
ApaLI G'TGCA_C
Cuts at: 3618 7067 8223 3618
Size: 3449 1156 4405
Fragments arranged by size:
4405 3449 1156
ApoI r'AATT_y
Cuts at: 1493 2203 2423 2520 2818 3045 4641 4811 5360
Size: 710 220 97 298 227 1596 170 549
Cuts at: 5360 5867 6274 6319 6545 6697 8806 1493
Size: 507 407 45 226 152 2109 1697
Fragments arranged by size:
2109 1697 1596 710 549 507 407 298
227 226 220 170 152 97 45
AseI AT'TA_AT
Cuts at: 160 1032 2688 3213 3813 3891 4446 7564 160
Size: 872 1656 525 600 78 555 3118 1606
Fragments arranged by size:
3118 1656 1606 872 600 555 525 78
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9010
AvaI C'yCGr_G
Cuts at: 1870 1943 6335 1870
Size: 73 4392 4545
Fragments arranged by size:
4545 4392 73
AvrII C'CTAG_G
Cuts at: 3434 6218 3434
Size: 2784 6226
BamHI G'GATC_C
Cuts at: 6340 6707 6340
Size: 367 8643
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3386 3828 6331 7786 618
Size: 325 217 627 1599 442 2503 1455 1842
Fragments arranged by size:
2503 1842 1599 1455 627 442 325 217
BanII G_rGCy'C
Cuts at: 1391 2896 4550 6329 1391
Size: 1505 1654 1779 4072
Fragments arranged by size:
4072 1779 1654 1505
BbeI G_GCGC'C
Cuts at: 3832 3832
Size: 9010
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5760 5936 928
Size: 971 3861 176 4002
Fragments arranged by size:
4002 3861 971 176
BbvCI CC'TCA_GC
Cuts at: 2114 2244 4921 2114
Size: 130 2677 6203
Fragments arranged by size:
6203 2677 130
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7201 7235 1903
Size: 34 5264 34 3678
Fragments arranged by size:
5264 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3255 3454 6902 8112 3255
Size: 199 3448 1210 4153
Fragments arranged by size:
4153 3448 1210 199
BclI T'GATC_A
Cuts at: 1548 3034 3361 4099 6291 1548
Size: 1486 327 738 2192 4267
Fragments arranged by size:
4267 2192 1486 738 327
BfrBI ATG'CAT
Cuts at: 3686 3686
Size: 9010
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7621 136
Size: 107 122 71 631 605 5949 1525
Fragments arranged by size:
5949 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2960 9005 2960
Size: 6045 2965
BlpI GC'TnA_GC
Cuts at: 6427 6427
Size: 9010
Bme1580I G_kGCm'C
Cuts at: 1790 3622 7071 8227 1790
Size: 1832 3449 1156 2573
Fragments arranged by size:
3449 2573 1832 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9010
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2005 6741 7699
Size: 416 53 83 399 24 588 4736 958
Cuts at: 7699 442
Size: 1753
Fragments arranged by size:
4736 1753 958 588 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9010
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3171 3171
Size: 9010
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2271 2735 7670 1239
Size: 1032 464 4935 2579
Fragments arranged by size:
4935 2579 1032 464
Bpu10I CC'TnA_GC
Cuts at: 2114 2244 4921 2114
Size: 130 2677 6203
Fragments arranged by size:
6203 2677 130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4163 4627 5884 7135 8000 8298 4163
Size: 464 1257 1251 865 298 4875
Fragments arranged by size:
4875 1257 1251 865 464 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3798 7673 882
Size: 617 2299 3875 2219
Fragments arranged by size:
3875 2299 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2194 5114 6801 493
Size: 711 990 2920 1687 2702
Fragments arranged by size:
2920 2702 1687 990 711
BsaBI GATnn'nnATC
Cuts at: 3606 6296 9004 3606
Size: 2690 2708 3612
Fragments arranged by size:
3612 2708 2690
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3829 6817 7199 7834
Size: 53 83 186 936 2296 2988 382 635
Cuts at: 7834 275
Size: 1451
Fragments arranged by size:
2988 2296 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2259 7443 7841 8115 8262 8673 2259
Size: 5184 398 274 147 411 2596
Fragments arranged by size:
5184 2596 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4410 4440 5076 5106 1178
Size: 30 165 30 3007 30 636 30 5082
Fragments arranged by size:
5082 3007 636 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 5310 1186
Size: 4124 4886
BseYI C'CCAG_C
Cuts at: 2235 8213 8709 2235
Size: 5978 496 2536
Fragments arranged by size:
5978 2536 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2048 2095 2625 3323 5114 5610 1163
Size: 573 312 47 530 698 1791 496 4563
Fragments arranged by size:
4563 1791 698 573 530 496 312 47
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6460 7221 8249 1540
Size: 275 186 4459 761 1028 2301
Fragments arranged by size:
4459 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2896 3433 3622 4576 7071 7156 8227 2896
Size: 537 189 954 2495 85 1071 3679
Fragments arranged by size:
3679 2495 1071 954 537 189 85
BsmI GAATG_Cn'
Cuts at: 6521 6614 6521
Size: 93 8917
BsmBI CGTCTCn'nnnn_
Cuts at: 2108 2108
Size: 9010
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2896 3433 3622 4550 4576 6329 7071
Size: 399 1106 537 189 928 26 1753 742
Cuts at: 7071 7156 8227 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1753 1106 1071 928 742 537 399
189 85 26
BspEI T'CCGG_A
Cuts at: 2259 8673 2259
Size: 6414 2596
BspHI T'CATG_A
Cuts at: 2419 6899 8629 2419
Size: 4480 1730 2800
Fragments arranged by size:
4480 2800 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 6365 844
Size: 5521 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2092 6897 1394
Size: 429 269 4805 3507
Fragments arranged by size:
4805 3507 429 269
BsrDI GCAATG_nn'
Cuts at: 65 1248 3375 3643 4328 7505 7679 65
Size: 1183 2127 268 685 3177 174 1396
Fragments arranged by size:
3177 2127 1396 1183 685 268 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7654 7841 1700
Size: 110 5844 187 2869
Fragments arranged by size:
5844 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2017 4979 96
Size: 1921 2962 4127
Fragments arranged by size:
4127 2962 1921
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9010
BssSI C'ACGA_G
Cuts at: 7070 8082 7070
Size: 1012 7998
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3386 3410 794
Size: 2592 24 6394
Fragments arranged by size:
6394 2592 24
BstBI TT'CG_AA
Cuts at: 3613 7814 3613
Size: 4201 4809
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9010
BstXI CCAn_nnnn'nTGG
Cuts at: 2736 2736
Size: 9010
BstYI r'GATC_y
Cuts at: 1972 2173 2731 2960 4714 6340 6707 7092 7109
Size: 201 558 229 1754 1626 367 385 17
Cuts at: 7109 8561 8647 8659 9005 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1754 1626 1452 558 385 367 346
229 201 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2366 3669 6097 513
Size: 552 939 362 1303 2428 3426
Fragments arranged by size:
3426 2428 1303 939 552 362
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8271
Fragments arranged by size:
8271 683 56
BtsI GCAGTG_nn'
Cuts at: 3411 4136 5211 5710 6526 7320 7340 3411
Size: 725 1075 499 816 794 20 5081
Fragments arranged by size:
5081 1075 816 794 725 499 20
ClaI AT'CG_AT
Cuts at: 1554 3712 1554
Size: 2158 6852
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2889 2924 4254 4289 529
Size: 35 2325 35 1330 35 5250
Fragments arranged by size:
5250 2325 1330 35 35 35
DraI TTT'AAA
Cuts at: 3543 5241 6314 3543
Size: 1698 1073 6239
Fragments arranged by size:
6239 1698 1073
DraIII CAC_nnn'GTG
Cuts at: 5371 5371
Size: 9010
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2051 4324 8017 809
Size: 507 735 2273 3693 1802
Fragments arranged by size:
3693 2273 1802 735 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4042 5508 6457
Size: 54 1425 50 275 18 2212 1466 949
Cuts at: 6457 7346 8975 8
Size: 889 1629 43
Fragments arranged by size:
2212 1629 1466 1425 949 889 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6457 1537
Size: 275 4645 4090
Fragments arranged by size:
4645 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4581 4668 6940 1353
Size: 3228 87 2272 3423
Fragments arranged by size:
3423 3228 2272 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3237 7597 7971 8117 387
Size: 1064 138 1648 4360 374 146 1280
Fragments arranged by size:
4360 1648 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2894 2894
Size: 9010
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2271 2381 2735 2888 4443 7073 7670
Size: 393 639 110 354 153 1555 2630 597
Cuts at: 7670 8457 8725 1239
Size: 787 268 1524
Fragments arranged by size:
2630 1555 1524 787 639 597 393 354
268 153 110
EcoNI CCTnn'n_nnAGG
Cuts at: 2301 2301
Size: 9010
EcoO109I rG'GnC_Cy
Cuts at: 3079 4649 3079
Size: 1570 7440
EcoRI G'AATT_C
Cuts at: 2520 2818 3045 5360 6274 6319 6697 2520
Size: 298 227 2315 914 45 378 4833
Fragments arranged by size:
4833 2315 914 378 298 227 45
EcoRV GAT'ATC
Cuts at: 3487 5545 3487
Size: 2058 6952
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2982 3371 3389 4325 5491 5631 2982
Size: 389 18 936 1166 140 6361
Fragments arranged by size:
6361 1166 936 389 140 18
FspI TGC'GCA
Cuts at: 7516 7516
Size: 9010
HaeII r_GCGC'y
Cuts at: 1678 3832 8157 1678
Size: 2154 4325 2531
Fragments arranged by size:
4325 2531 2154
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2716 2731 2748 2763 4103
Size: 32 135 32 1144 15 17 15 1340
Cuts at: 4103 4135 4464 4496 4667 4699 4955 4987 6148
Size: 32 329 32 171 32 256 32 1161
Cuts at: 6148 6180 7651 7683 7725 7757 1373
Size: 32 1471 32 42 32 2626
Fragments arranged by size:
2626 1471 1340 1161 1144 329 256 171
135 42 32 32 32 32 32 32
32 32 32 17 15 15
HincII GTy'rAC
Cuts at: 669 1657 1938 3591 4168 4317 5036 5670 5783
Size: 988 281 1653 577 149 719 634 113
Cuts at: 5783 6354 6600 669
Size: 571 246 3079
Fragments arranged by size:
3079 1653 988 719 634 577 571 281
246 149 113
HindIII A'AGCT_T
Cuts at: 748 2264 748
Size: 1516 7494
HpaI GTT'AAC
Cuts at: 4168 6600 4168
Size: 2432 6578
KasI G'GCGC_C
Cuts at: 3828 3828
Size: 9010
KpnI G_GTAC'C
Cuts at: 6335 6335
Size: 9010
MfeI C'AATT_G
Cuts at: 6022 6609 6022
Size: 587 8423
MluI A'CGCG_T
Cuts at: 8886 8886
Size: 9010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2889 4725 8124 8308 1305
Size: 1584 1836 3399 184 2007
Fragments arranged by size:
3399 2007 1836 1584 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4044 10
Size: 54 1425 343 2212 4976
Fragments arranged by size:
4976 2212 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2532 6109 6195 6968 7327 7486 518
Size: 648 1366 3577 86 773 359 159 2042
Fragments arranged by size:
3577 2042 1366 773 648 359 159 86
MspA1I CmG'CkG
Cuts at: 1606 3383 3853 3970 4061 5098 6172 6426 7103
Size: 1777 470 117 91 1037 1074 254 677
Cuts at: 7103 8251 8496 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1777 1148 1074 1037 677 470 254
245 117 91
NarI GG'CG_CC
Cuts at: 3829 3829
Size: 9010
NcoI C'CATG_G
Cuts at: 513 1065 2004 2366 3669 6097 513
Size: 552 939 362 1303 2428 3426
Fragments arranged by size:
3426 2428 1303 939 552 362
NdeI CA'TA_TG
Cuts at: 387 2782 3693 4804 387
Size: 2395 911 1111 4593
Fragments arranged by size:
4593 2395 1111 911
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9010
NotI GC'GGCC_GC
Cuts at: 6457 6457
Size: 9010
NsiI A_TGCA'T
Cuts at: 3688 3688
Size: 9010
NspI r_CATG'y
Cuts at: 1132 1229 2448 3021 3743 6183 6368 7913 1132
Size: 97 1219 573 722 2440 185 1545 2229
Fragments arranged by size:
2440 2229 1545 1219 722 573 185 97
PacI TTA_AT'TAA
Cuts at: 4446 4446
Size: 9010
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9010
PciI A'CATG_T
Cuts at: 7909 7909
Size: 9010
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4064 4841 1108
Size: 2956 777 5277
Fragments arranged by size:
5277 2956 777
PfoI T'CCnGG_A
Cuts at: 2040 4579 2040
Size: 2539 6471
PmeI GTTT'AAAC
Cuts at: 6314 6314
Size: 9010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4208 4240 7079 7111 8592 8624 4208
Size: 32 2839 32 1481 32 4594
Fragments arranged by size:
4594 2839 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4649 4649
Size: 9010
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9010
PsiI TTA'TAA
Cuts at: 3653 4527 6204 6580 3653
Size: 874 1677 376 6083
Fragments arranged by size:
6083 1677 874 376
PstI C_TGCA'G
Cuts at: 830 2302 3574 5264 6171 6362 830
Size: 1472 1272 1690 907 191 3478
Fragments arranged by size:
3478 1690 1472 1272 907 191
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9010
PvuII CAG'CTG
Cuts at: 3383 3853 3970 5098 6172 3383
Size: 470 117 1128 1074 6221
Fragments arranged by size:
6221 1128 1074 470 117
SacI G_AGCT'C
Cuts at: 2896 2896
Size: 9010
SalI G'TCGA_C
Cuts at: 1936 6352 1936
Size: 4416 4594
SapI GCTCTTCn'nnn_
Cuts at: 4581 4581
Size: 9010
SbfI CC_TGCA'GG
Cuts at: 6362 6362
Size: 9010
ScaI AGT'ACT
Cuts at: 7258 8970 7258
Size: 1712 7298
SfcI C'TryA_G
Cuts at: 826 1045 2298 3570 4770 5007 5260 5805 6167
Size: 219 1253 1272 1200 237 253 545 362
Cuts at: 6167 6358 7493 8174 8365 826
Size: 191 1135 681 191 1471
Fragments arranged by size:
1471 1272 1253 1200 1135 681 545 362
253 237 219 191 191
SfoI GGC'GCC
Cuts at: 3830 3830
Size: 9010
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9010
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9010
SmaI CCC'GGG
Cuts at: 1872 6337 1872
Size: 4465 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2132 4142 4606 5899 7114 8015
Size: 197 926 189 2010 464 1293 1215 901
Cuts at: 8015 8277 820
Size: 262 1553
Fragments arranged by size:
2010 1553 1293 1215 926 901 464 262
197 189
SnaBI TAC'GTA
Cuts at: 493 6801 493
Size: 6308 2702
SpeI A'CTAG_T
Cuts at: 152 4390 5339 152
Size: 4238 949 3823
Fragments arranged by size:
4238 3823 949
SphI G_CATG'C
Cuts at: 1132 3743 6183 6368 1132
Size: 2611 2440 185 3774
Fragments arranged by size:
3774 2611 2440 185
SspI AAT'ATT
Cuts at: 5 52 3560 4601 5202 5722 6934 5
Size: 47 3508 1041 601 520 1212 2081
Fragments arranged by size:
3508 2081 1212 1041 601 520 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9010
StyI C'CwwG_G
Cuts at: 513 1065 2004 2366 2735 3390 3434 3669 6097
Size: 552 939 362 369 655 44 235 2428
Cuts at: 6097 6208 6218 6449 513
Size: 111 10 231 3074
Fragments arranged by size:
3074 2428 939 655 552 369 362 235
231 111 44 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2249 2913 5223 7037 7054 7207 7392
Size: 1255 669 664 2310 1814 17 153 185
Cuts at: 7392 7837 325
Size: 445 1498
Fragments arranged by size:
2310 1814 1498 1255 669 664 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2017 2584 2798
Size: 274 80 33 51 1443 40 567 214
Cuts at: 2798 4979 7256 8968 96
Size: 2181 2277 1712 138
Fragments arranged by size:
2277 2181 1712 1443 567 274 214 138
80 51 40 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5076 5108 6078 6110 8970 9002 5076
Size: 32 970 32 2860 32 5084
Fragments arranged by size:
5084 2860 970 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9010
XbaI T'CTAG_A
Cuts at: 6346 6346
Size: 9010
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9010
XmaI C'CCGG_G
Cuts at: 1870 6335 1870
Size: 4465 4545
XmnI GAAnn'nnTTC
Cuts at: 2391 4600 5269 5870 7139 2391
Size: 2209 669 601 1269 4262
Fragments arranged by size:
4262 2209 1269 669 601
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6818 276
Size: 53 83 186 936 5284 2468
Fragments arranged by size:
5284 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BsiWI BstZ17I
Bsu36I FseI FspAI NaeI NgoMIV NruI PmlI PspOMI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI
SwaI XcmI