Restriction map for
pFN21AA1009
(Circular) (Six-base) MAPSORT of: pFN21AA1009.seq Check: 7896 from: 1 to: 8686
pFN21AA1009 8686 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4959 6496 278
Size: 53 83 186 936 3423 1537 2468
Fragments arranged by size:
3423 2468 1537 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3515 4227 6029 1937
Size: 1578 712 1802 4594
Fragments arranged by size:
4594 1802 1578 712
Acc65I G'GTAC_C
Cuts at: 3232 6007 3232
Size: 2775 5911
AclI AA'CG_TT
Cuts at: 1563 6813 7186 1563
Size: 5250 373 3063
Fragments arranged by size:
5250 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2960 3090 3222 3357 4601 5093 5375 5388
Size: 1328 130 132 135 1244 492 282 13
Cuts at: 5388 5637 6749 8133 8401 1632
Size: 249 1112 1384 268 1917
Fragments arranged by size:
1917 1384 1328 1244 1112 492 282 268
249 135 132 130 13
AflII C'TTAA_G
Cuts at: 820 1017 2045 2068 820
Size: 197 1028 23 7438
Fragments arranged by size:
7438 1028 197 23
AflIII A'CryG_T
Cuts at: 1203 2977 3091 7585 8562 1203
Size: 1774 114 4494 977 1327
Fragments arranged by size:
4494 1774 1327 977 114
AgeI A'CCGG_T
Cuts at: 7517 7517
Size: 8686
AhdI GACnn_n'nnGTC
Cuts at: 7415 7415
Size: 8686
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8686
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4827 1184
Size: 3643 5043
AlwNI CAG_nnn'CTG
Cuts at: 3548 4546 4634 6429 8001 3548
Size: 998 88 1795 1572 4233
Fragments arranged by size:
4233 1795 1572 998 88
ApaLI G'TGCA_C
Cuts at: 2385 3255 6743 7899 2385
Size: 870 3488 1156 3172
Fragments arranged by size:
3488 3172 1156 870
ApoI r'AATT_y
Cuts at: 1493 2428 2773 2918 4123 4311 4536 4664 5000
Size: 935 345 145 1205 188 225 128 336
Cuts at: 5000 5497 5631 5995 6221 6373 8482 1493
Size: 497 134 364 226 152 2109 1697
Fragments arranged by size:
2109 1697 1205 935 497 364 345 336
226 225 188 152 145 134 128
AseI AT'TA_AT
Cuts at: 160 1032 2280 3171 5333 7240 160
Size: 872 1248 891 2162 1907 1606
Fragments arranged by size:
2162 1907 1606 1248 891 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8686
AvaI C'yCGr_G
Cuts at: 1870 1943 6011 1870
Size: 73 4068 4545
Fragments arranged by size:
4545 4068 73
AvrII C'CTAG_G
Cuts at: 3428 3428
Size: 8686
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3577 3610 3577
Size: 33 8653
BamHI G'GATC_C
Cuts at: 6016 6383 6016
Size: 367 8319
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2571 3232 6007 7462 618
Size: 325 217 627 784 661 2775 1455 1842
Fragments arranged by size:
2775 1842 1455 784 661 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2115 2741 3314 4110 6005 1391
Size: 724 626 573 796 1895 4072
Fragments arranged by size:
4072 1895 796 724 626 573
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2700 4191 928
Size: 971 801 1491 5423
Fragments arranged by size:
5423 1491 971 801
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4055 4089 6877 6911 1903
Size: 34 2118 34 2788 34 3678
Fragments arranged by size:
3678 2788 2118 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3578 6578 7788 3578
Size: 3000 1210 4476
Fragments arranged by size:
4476 3000 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8686
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5177 7297 136
Size: 107 122 71 631 605 3505 2120 1525
Fragments arranged by size:
3505 2120 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3185 3973 4132 8681 3185
Size: 788 159 4549 3190
Fragments arranged by size:
4549 3190 788 159
BlpI GC'TnA_GC
Cuts at: 6103 6103
Size: 8686
Bme1580I G_kGCm'C
Cuts at: 1790 2389 2457 3259 6747 7903 1790
Size: 599 68 802 3488 1156 2573
Fragments arranged by size:
3488 2573 1156 802 599 68
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8686
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3324 6417 7375
Size: 416 53 83 399 24 1907 3093 958
Cuts at: 7375 442
Size: 1753
Fragments arranged by size:
3093 1907 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8686
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3894 4568 4724 5894 7346 1239
Size: 2655 674 156 1170 1452 2579
Fragments arranged by size:
2655 2579 1452 1170 674 156
Bpu10I CC'TnA_GC
Cuts at: 2562 3313 2562
Size: 751 7935
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2210 2742 2949 3326 3421 4071 4469 5185 5526
Size: 532 207 377 95 650 398 716 341
Cuts at: 5526 6811 7676 7974 2210
Size: 1285 865 298 2922
Fragments arranged by size:
2922 1285 865 716 650 532 398 377
341 298 207 95
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7349 882
Size: 617 5850 2219
Fragments arranged by size:
5850 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6477 493
Size: 711 5273 2702
Fragments arranged by size:
5273 2702 711
BsaBI GATnn'nnATC
Cuts at: 8680 8680
Size: 8686
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4956 6493 6875 7510
Size: 53 83 186 936 3423 1537 382 635
Cuts at: 7510 275
Size: 1451
Fragments arranged by size:
3423 1537 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 7119 7517 7791 7938 8349 7119
Size: 398 274 147 411 7456
Fragments arranged by size:
7456 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3995 4025 5928 5958 1178
Size: 30 165 30 2592 30 1903 30 3906
Fragments arranged by size:
3906 2592 1903 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2177 2834 4016 1186
Size: 847 144 657 1182 5856
Fragments arranged by size:
5856 1182 847 657 144
BseYI C'CCAG_C
Cuts at: 7889 8385 7889
Size: 496 8190
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5909 1163
Size: 573 4173 3940
Fragments arranged by size:
4173 3940 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6136 6897 7925 1540
Size: 275 186 4135 761 1028 2301
Fragments arranged by size:
4135 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2115 2389 2741 3259 3314 6747 6832 7903 2115
Size: 274 352 518 55 3433 85 1071 2898
Fragments arranged by size:
3433 2898 1071 518 352 274 85 55
BsmI GAATG_Cn'
Cuts at: 4948 5977 6197 6290 4948
Size: 1029 220 93 7344
Fragments arranged by size:
7344 1029 220 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2115 2389 2457 2741 3259 3314 4110
Size: 399 325 274 68 284 518 55 796
Cuts at: 4110 6005 6747 6832 7903 1391
Size: 1895 742 85 1071 2174
Fragments arranged by size:
2174 1895 1071 796 742 518 399 325
284 274 85 68 55
BspEI T'CCGG_A
Cuts at: 8349 8349
Size: 8686
BspHI T'CATG_A
Cuts at: 6575 8305 6575
Size: 1730 6956
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4615 6041 844
Size: 3771 1426 3489
Fragments arranged by size:
3771 3489 1426
BsrBI CCG'CTC
Cuts at: 1394 1823 6573 1394
Size: 429 4750 3507
Fragments arranged by size:
4750 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5430 7181 7355 65
Size: 1183 4182 1751 174 1396
Fragments arranged by size:
4182 1751 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5928 7330 7517 1700
Size: 110 4118 1402 187 2869
Fragments arranged by size:
4118 2869 1402 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8686
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8686
BssSI C'ACGA_G
Cuts at: 6746 7758 6746
Size: 1012 7674
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4526 5432 794
Size: 3732 906 4048
Fragments arranged by size:
4048 3732 906
BstBI TT'CG_AA
Cuts at: 4233 7490 4233
Size: 3257 5429
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8686
BstYI r'GATC_y
Cuts at: 1972 3185 3973 4132 4543 6016 6383 6768 6785
Size: 1213 788 159 411 1473 367 385 17
Cuts at: 6785 8237 8323 8335 8681 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1473 1452 1213 788 411 385 367
346 159 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3580 3580
Size: 8686
BtgI C'CryG_G
Cuts at: 513 1065 2004 4743 513
Size: 552 939 2739 4456
Fragments arranged by size:
4456 2739 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7947
Fragments arranged by size:
7947 683 56
BtsI GCAGTG_nn'
Cuts at: 2059 5379 6202 6996 7016 2059
Size: 3320 823 794 20 3729
Fragments arranged by size:
3729 3320 823 794 20
ClaI AT'CG_AT
Cuts at: 1554 3407 1554
Size: 1853 6833
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5079 5114 529
Size: 35 4515 35 4101
Fragments arranged by size:
4515 4101 35 35
DraI TTT'AAA
Cuts at: 2266 3625 5990 2266
Size: 1359 2365 4962
Fragments arranged by size:
4962 2365 1359
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7693 809
Size: 507 6377 1802
Fragments arranged by size:
6377 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2898 6133 7022
Size: 54 1425 50 275 18 1068 3235 889
Cuts at: 7022 8651 8
Size: 1629 43
Fragments arranged by size:
3235 1629 1425 1068 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6133 1537
Size: 275 4321 4090
Fragments arranged by size:
4321 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2010 2403 2763 3767 4577 6616 1353
Size: 657 393 360 1004 810 2039 3423
Fragments arranged by size:
3423 2039 1004 810 657 393 360
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7273 7647 7793 387
Size: 1064 138 5684 374 146 1280
Fragments arranged by size:
5684 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2113 2739 3312 2113
Size: 626 573 7487
Fragments arranged by size:
7487 626 573
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2960 3090 3222 3357 3894 4568 4601
Size: 393 1328 130 132 135 537 674 33
Cuts at: 4601 4724 5093 5375 5388 5637 5894 6749 7346
Size: 123 369 282 13 249 257 855 597
Cuts at: 7346 8133 8401 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1328 855 787 674 597 537 393
369 282 268 257 249 135 132 130
123 33 13
EcoNI CCTnn'n_nnAGG
Cuts at: 4859 4859
Size: 8686
EcoRI G'AATT_C
Cuts at: 2428 2773 5000 5995 6373 2428
Size: 345 2227 995 378 4741
Fragments arranged by size:
4741 2227 995 378 345
EcoRV GAT'ATC
Cuts at: 2731 3935 4592 2731
Size: 1204 657 6825
Fragments arranged by size:
6825 1204 657
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3938 4421 4522 5567 3938
Size: 483 101 1045 7057
Fragments arranged by size:
7057 1045 483 101
FspI TGC'GCA
Cuts at: 3663 7192 3663
Size: 3529 5157
HaeII r_GCGC'y
Cuts at: 1678 7833 1678
Size: 6155 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2309 2341 4576 4608 4976
Size: 32 135 32 737 32 2235 32 368
Cuts at: 4976 5008 5150 5182 7327 7359 7401 7433 1373
Size: 32 142 32 2145 32 42 32 2626
Fragments arranged by size:
2626 2235 2145 737 368 142 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2394 6030 6276 669
Size: 988 281 456 3636 246 3079
Fragments arranged by size:
3636 3079 988 456 281 246
HindIII A'AGCT_T
Cuts at: 748 2268 2658 748
Size: 1520 390 6776
Fragments arranged by size:
6776 1520 390
HpaI GTT'AAC
Cuts at: 6276 6276
Size: 8686
KpnI G_GTAC'C
Cuts at: 3236 6011 3236
Size: 2775 5911
MfeI C'AATT_G
Cuts at: 6285 6285
Size: 8686
MluI A'CGCG_T
Cuts at: 8562 8562
Size: 8686
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3708 7800 7984 1305
Size: 2403 4092 184 2007
Fragments arranged by size:
4092 2403 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6864
Fragments arranged by size:
6864 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2319 4941 6644 7003 7162 518
Size: 648 1153 2622 1703 359 159 2042
Fragments arranged by size:
2622 2042 1703 1153 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3054 3860 4634 6102 6779 7927 8172 1606
Size: 1448 806 774 1468 677 1148 245 2120
Fragments arranged by size:
2120 1468 1448 1148 806 774 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 4743 513
Size: 552 939 2739 4456
Fragments arranged by size:
4456 2739 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8686
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8686
NotI GC'GGCC_GC
Cuts at: 6133 6133
Size: 8686
NspI r_CATG'y
Cuts at: 1132 1229 2485 2981 3095 5756 6044 7589 1132
Size: 97 1256 496 114 2661 288 1545 2229
Fragments arranged by size:
2661 2229 1545 1256 496 288 114 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8686
PciI A'CATG_T
Cuts at: 2977 3091 7585 2977
Size: 114 4494 4078
Fragments arranged by size:
4494 4078 114
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2683 1108
Size: 1575 7111
PmeI GTTT'AAAC
Cuts at: 5990 5990
Size: 8686
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6755 6787 8268 8300 6755
Size: 32 1481 32 7141
Fragments arranged by size:
7141 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8686
PsiI TTA'TAA
Cuts at: 3386 5555 6256 3386
Size: 2169 701 5816
Fragments arranged by size:
5816 2169 701
PstI C_TGCA'G
Cuts at: 830 3475 5425 5589 6038 830
Size: 2645 1950 164 449 3478
Fragments arranged by size:
3478 2645 1950 449 164
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8686
PvuII CAG'CTG
Cuts at: 3054 3860 4634 3054
Size: 806 774 7106
Fragments arranged by size:
7106 806 774
SacI G_AGCT'C
Cuts at: 2115 2741 3314 2115
Size: 626 573 7487
Fragments arranged by size:
7487 626 573
SalI G'TCGA_C
Cuts at: 1936 6028 1936
Size: 4092 4594
SbfI CC_TGCA'GG
Cuts at: 6038 6038
Size: 8686
ScaI AGT'ACT
Cuts at: 2074 6934 8646 2074
Size: 4860 1712 2114
Fragments arranged by size:
4860 2114 1712
SfcI C'TryA_G
Cuts at: 826 1045 3471 4995 5305 5421 5585 5636 6034
Size: 219 2426 1524 310 116 164 51 398
Cuts at: 6034 7169 7850 8041 826
Size: 1135 681 191 1471
Fragments arranged by size:
2426 1524 1471 1135 681 398 310 219
191 164 116 51
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8686
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8686
SmaI CCC'GGG
Cuts at: 1872 6013 1872
Size: 4141 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2045 2068 2189 2757 2964 3341
Size: 197 926 102 23 121 568 207 377
Cuts at: 3341 3400 4086 4448 5200 5541 6790 7691 7953
Size: 59 686 362 752 341 1249 901 262
Cuts at: 7953 820
Size: 1553
Fragments arranged by size:
1553 1249 926 901 752 686 568 377
362 341 262 207 197 121 102 59
23
SnaBI TAC'GTA
Cuts at: 493 6477 493
Size: 5984 2702
SpeI A'CTAG_T
Cuts at: 152 2098 152
Size: 1946 6740
SphI G_CATG'C
Cuts at: 1132 2485 6044 1132
Size: 1353 3559 3774
Fragments arranged by size:
3774 3559 1353
SspI AAT'ATT
Cuts at: 5 52 2956 3115 4617 5904 6610 5
Size: 47 2904 159 1502 1287 706 2081
Fragments arranged by size:
2904 2081 1502 1287 706 159 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8686
StyI C'CwwG_G
Cuts at: 513 1065 2004 2567 3428 4743 6125 513
Size: 552 939 563 861 1315 1382 3074
Fragments arranged by size:
3074 1382 1315 939 861 563 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6713 6730 6883 7068 7513 325
Size: 1255 5133 17 153 185 445 1498
Fragments arranged by size:
5133 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2072 6932 8644
Size: 274 80 33 51 1443 95 4860 1712
Cuts at: 8644 96
Size: 138
Fragments arranged by size:
4860 1712 1443 274 138 95 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5420 5452 5928 5960 8646 8678 5420
Size: 32 476 32 2686 32 5428
Fragments arranged by size:
5428 2686 476 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8686
XbaI T'CTAG_A
Cuts at: 6022 6022
Size: 8686
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8686
XmaI C'CCGG_G
Cuts at: 1870 6011 1870
Size: 4141 4545
XmnI GAAnn'nnTTC
Cuts at: 2034 2845 4575 4986 5631 5708 6815 2034
Size: 811 1730 411 645 77 1107 3905
Fragments arranged by size:
3905 1730 1107 811 645 411 77
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4957 6494 276
Size: 53 83 186 936 3423 1537 2468
Fragments arranged by size:
3423 2468 1537 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoO109I FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI