Restriction map for
pFN21AA1008
(Circular) (Six-base) MAPSORT of: pFN21AA1008.seq Check: 4538 from: 1 to: 7489
pFN21AA1008 7489 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3046 3046
Size: 7489
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5299 278
Size: 53 83 186 936 3763 2468
Fragments arranged by size:
3763 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3348 4832 1937
Size: 1411 1484 4594
Fragments arranged by size:
4594 1484 1411
Acc65I G'GTAC_C
Cuts at: 4810 4810
Size: 7489
AclI AA'CG_TT
Cuts at: 1563 5616 5989 1563
Size: 4053 373 3063
Fragments arranged by size:
4053 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2580 3708 3794 3840 5552 6936 7204 1632
Size: 948 1128 86 46 1712 1384 268 1917
Fragments arranged by size:
1917 1712 1384 1128 948 268 86 46
AflII C'TTAA_G
Cuts at: 820 1017 2614 2903 820
Size: 197 1597 289 5406
Fragments arranged by size:
5406 1597 289 197
AflIII A'CryG_T
Cuts at: 1203 6388 7365 1203
Size: 5185 977 1327
Fragments arranged by size:
5185 1327 977
AgeI A'CCGG_T
Cuts at: 6320 6320
Size: 7489
AhdI GACnn_n'nnGTC
Cuts at: 6218 6218
Size: 7489
AleI CACnn'nnGTG
Cuts at: 1166 2216 1166
Size: 1050 6439
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7489
AlwNI CAG_nnn'CTG
Cuts at: 2181 5232 6804 2181
Size: 3051 1572 2866
Fragments arranged by size:
3051 2866 1572
ApaLI G'TGCA_C
Cuts at: 5546 6702 5546
Size: 1156 6333
ApoI r'AATT_y
Cuts at: 1493 3060 3136 3169 3409 3768 3892 3908 3945
Size: 1567 76 33 240 359 124 16 37
Cuts at: 3945 3956 4014 4042 4366 4798 5024 5176 7285
Size: 11 58 28 324 432 226 152 2109
Cuts at: 7285 1493
Size: 1697
Fragments arranged by size:
2109 1697 1567 432 359 324 240 226
152 124 76 58 37 33 28 16
11
AseI AT'TA_AT
Cuts at: 160 1032 3660 4502 6043 160
Size: 872 2628 842 1541 1606
Fragments arranged by size:
2628 1606 1541 872 842
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7489
AvaI C'yCGr_G
Cuts at: 1870 1943 2037 2105 4814 1870
Size: 73 94 68 2709 4545
Fragments arranged by size:
4545 2709 94 73 68
BamHI G'GATC_C
Cuts at: 4819 5186 4819
Size: 367 7122
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4810 6265 618
Size: 325 217 627 3023 1455 1842
Fragments arranged by size:
3023 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4808 1391
Size: 3417 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3028 3324 928
Size: 971 1129 296 5093
Fragments arranged by size:
5093 1129 971 296
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5680 5714 1903
Size: 34 50 34 3659 34 3678
Fragments arranged by size:
3678 3659 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5381 6591 5381
Size: 1210 6279
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7489
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6100 136
Size: 107 122 71 631 605 4428 1525
Fragments arranged by size:
4428 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3862 7484 3862
Size: 3622 3867
BlpI GC'TnA_GC
Cuts at: 3545 4213 4906 3545
Size: 668 693 6128
Fragments arranged by size:
6128 693 668
Bme1580I G_kGCm'C
Cuts at: 1790 5550 6706 1790
Size: 3760 1156 2573
Fragments arranged by size:
3760 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3577 1318
Size: 2259 5230
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4417 5220 6178
Size: 416 53 83 399 24 3000 803 958
Cuts at: 6178 442
Size: 1753
Fragments arranged by size:
3000 1753 958 803 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2676 1056
Size: 1620 5869
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3072 4606 3072
Size: 1534 5955
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2168 2260 2266 3304 6149 1239
Size: 929 92 6 1038 2845 2579
Fragments arranged by size:
2845 2579 1038 929 92 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1994 5614 6479 6777 1994
Size: 3620 865 298 2706
Fragments arranged by size:
3620 2706 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2968 6152 882
Size: 617 1469 3184 2219
Fragments arranged by size:
3184 2219 1469 617
BsaAI yAC'GTr
Cuts at: 493 1204 5280 493
Size: 711 4076 2702
Fragments arranged by size:
4076 2702 711
BsaBI GATnn'nnATC
Cuts at: 2057 4200 7483 2057
Size: 2143 3283 2063
Fragments arranged by size:
3283 2143 2063
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5296 5678 6313 275
Size: 53 83 186 936 3763 382 635 1451
Fragments arranged by size:
3763 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5922 6320 6594 6741 7152 5922
Size: 398 274 147 411 6259
Fragments arranged by size:
6259 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7264
Fragments arranged by size:
7264 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7489
BseYI C'CCAG_C
Cuts at: 6692 7188 6692
Size: 496 6993
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2210 1163
Size: 573 474 6442
Fragments arranged by size:
6442 573 474
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4939 5700 6728 1540
Size: 275 186 2938 761 1028 2301
Fragments arranged by size:
2938 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2073 5550 5635 6706 2073
Size: 3477 85 1071 2856
Fragments arranged by size:
3477 2856 1071 85
BsmI GAATG_Cn'
Cuts at: 2904 4492 4722 5000 5093 2904
Size: 1588 230 278 93 5300
Fragments arranged by size:
5300 1588 278 230 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2076 4224 2076
Size: 2148 5341
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 4808 5550 5635 6706 1391
Size: 399 283 2735 742 85 1071 2174
Fragments arranged by size:
2735 2174 1071 742 399 283 85
BspEI T'CCGG_A
Cuts at: 7152 7152
Size: 7489
BspHI T'CATG_A
Cuts at: 3949 5378 7108 3949
Size: 1429 1730 4330
Fragments arranged by size:
4330 1730 1429
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2085 3046 4844 844
Size: 1241 961 1798 3489
Fragments arranged by size:
3489 1798 1241 961
BsrBI CCG'CTC
Cuts at: 1394 1823 5376 1394
Size: 429 3553 3507
Fragments arranged by size:
3553 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5984 6158 65
Size: 1183 4736 174 1396
Fragments arranged by size:
4736 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2140 6133 6320 1700
Size: 110 330 3993 187 2869
Fragments arranged by size:
3993 2869 330 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7489
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7489
BssSI C'ACGA_G
Cuts at: 2132 5549 6561 2132
Size: 3417 1012 3060
Fragments arranged by size:
3417 3060 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2077 2181 2200 794
Size: 1283 104 19 6083
Fragments arranged by size:
6083 1283 104 19
BstBI TT'CG_AA
Cuts at: 3979 6293 3979
Size: 2314 5175
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7489
BstXI CCAn_nnnn'nTGG
Cuts at: 4075 4075
Size: 7489
BstYI r'GATC_y
Cuts at: 1972 3862 4680 4819 5186 5571 5588 7040 7126
Size: 1890 818 139 367 385 17 1452 86
Cuts at: 7126 7138 7484 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1890 1452 818 385 367 346 139
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3323 3323
Size: 7489
BtgI C'CryG_G
Cuts at: 513 1065 3745 3897 513
Size: 552 2680 152 4105
Fragments arranged by size:
4105 2680 552 152
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6750
Fragments arranged by size:
6750 683 56
BtsI GCAGTG_nn'
Cuts at: 5005 5799 5819 5005
Size: 794 20 6675
Fragments arranged by size:
6675 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7489
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7454
DraI TTT'AAA
Cuts at: 2763 4793 2763
Size: 2030 5459
DraIII CAC_nnn'GTG
Cuts at: 3163 3163
Size: 7489
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6496 809
Size: 507 5180 1802
Fragments arranged by size:
5180 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4936 5825 7454
Size: 54 1425 50 275 18 3106 889 1629
Cuts at: 7454 8
Size: 43
Fragments arranged by size:
3106 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4936 1537
Size: 275 3124 4090
Fragments arranged by size:
4090 3124 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2880 3484 5419 1353
Size: 1527 604 1935 3423
Fragments arranged by size:
3423 1935 1527 604
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6076 6450 6596 387
Size: 1064 138 4487 374 146 1280
Fragments arranged by size:
4487 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2168 2260 2266 2580 3304 3708 3794
Size: 393 536 92 6 314 724 404 86
Cuts at: 3794 3840 5552 6149 6936 7204 1239
Size: 46 1712 597 787 268 1524
Fragments arranged by size:
1712 1524 787 724 597 536 404 393
314 268 92 86 46 6
EcoO109I rG'GnC_Cy
Cuts at: 2164 2256 2164
Size: 92 7397
EcoRI G'AATT_C
Cuts at: 3060 4798 5176 3060
Size: 1738 378 5373
Fragments arranged by size:
5373 1738 378
EcoRV GAT'ATC
Cuts at: 3146 3146
Size: 7489
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2041 2041
Size: 7489
FseI GG_CCGG'CC
Cuts at: 2144 2144
Size: 7489
FspI TGC'GCA
Cuts at: 2113 5995 2113
Size: 3882 3607
HaeII r_GCGC'y
Cuts at: 1678 6636 1678
Size: 4958 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2073 2105 3464 3496 6130
Size: 32 135 32 501 32 1359 32 2634
Cuts at: 6130 6162 6204 6236 1373
Size: 32 42 32 2626
Fragments arranged by size:
2634 2626 1359 501 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4326 4833 5079 669
Size: 988 281 2388 507 246 3079
Fragments arranged by size:
3079 2388 988 507 281 246
HindIII A'AGCT_T
Cuts at: 748 4340 4778 748
Size: 3592 438 3459
Fragments arranged by size:
3592 3459 438
HpaI GTT'AAC
Cuts at: 5079 5079
Size: 7489
KpnI G_GTAC'C
Cuts at: 4814 4814
Size: 7489
MfeI C'AATT_G
Cuts at: 5088 5088
Size: 7489
MluI A'CGCG_T
Cuts at: 7365 7365
Size: 7489
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3163 6603 6787 1305
Size: 1858 3440 184 2007
Fragments arranged by size:
3440 2007 1858 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5667
Fragments arranged by size:
5667 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2216 3358 3595 4206 5447 5806 5965
Size: 648 1050 1142 237 611 1241 359 159
Cuts at: 5965 518
Size: 2042
Fragments arranged by size:
2042 1241 1142 1050 648 611 359 237
159
MspA1I CmG'CkG
Cuts at: 1606 2419 4905 5582 6730 6975 1606
Size: 813 2486 677 1148 245 2120
Fragments arranged by size:
2486 2120 1148 813 677 245
NaeI GCC'GGC
Cuts at: 2142 2142
Size: 7489
NcoI C'CATG_G
Cuts at: 513 1065 3897 513
Size: 552 2832 4105
Fragments arranged by size:
4105 2832 552
NdeI CA'TA_TG
Cuts at: 387 3682 387
Size: 3295 4194
NgoMIV G'CCGG_C
Cuts at: 2140 2140
Size: 7489
NheI G'CTAG_C
Cuts at: 1052 2672 1052
Size: 1620 5869
NotI GC'GGCC_GC
Cuts at: 4936 4936
Size: 7489
NspI r_CATG'y
Cuts at: 1132 1229 2992 3971 4847 6392 1132
Size: 97 1763 979 876 1545 2229
Fragments arranged by size:
2229 1763 1545 979 876 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7489
PciI A'CATG_T
Cuts at: 6388 6388
Size: 7489
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2984 4514 1108
Size: 1876 1530 4083
Fragments arranged by size:
4083 1876 1530
PmeI GTTT'AAAC
Cuts at: 4793 4793
Size: 7489
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5558 5590 7071 7103 5558
Size: 32 1481 32 5944
Fragments arranged by size:
5944 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2164 2256 2164
Size: 92 7397
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7489
PsiI TTA'TAA
Cuts at: 5059 5059
Size: 7489
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4692 4724 4692
Size: 32 7457
PstI C_TGCA'G
Cuts at: 830 3870 4841 830
Size: 3040 971 3478
Fragments arranged by size:
3478 3040 971
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7489
PvuII CAG'CTG
Cuts at: 2419 2419
Size: 7489
SalI G'TCGA_C
Cuts at: 1936 4831 1936
Size: 2895 4594
SbfI CC_TGCA'GG
Cuts at: 4841 4841
Size: 7489
ScaI AGT'ACT
Cuts at: 5737 7449 5737
Size: 1712 5777
SfcI C'TryA_G
Cuts at: 826 1045 2624 2697 2937 3858 3866 4837 5972
Size: 219 1579 73 240 921 8 971 1135
Cuts at: 5972 6653 6844 826
Size: 681 191 1471
Fragments arranged by size:
1579 1471 1135 971 921 681 240 219
191 73 8
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7489
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7489
SmaI CCC'GGG
Cuts at: 1872 2039 2107 4816 1872
Size: 167 68 2709 4545
Fragments arranged by size:
4545 2709 167 68
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2009 2614 2903 5593 6494 6756
Size: 197 926 66 605 289 2690 901 262
Cuts at: 6756 820
Size: 1553
Fragments arranged by size:
2690 1553 926 901 605 289 262 197
66
SnaBI TAC'GTA
Cuts at: 493 5280 493
Size: 4787 2702
SpeI A'CTAG_T
Cuts at: 152 3737 152
Size: 3585 3904
SphI G_CATG'C
Cuts at: 1132 2992 4847 1132
Size: 1860 1855 3774
Fragments arranged by size:
3774 1860 1855
SspI AAT'ATT
Cuts at: 5 52 2595 3928 4140 5413 5
Size: 47 2543 1333 212 1273 2081
Fragments arranged by size:
2543 2081 1333 1273 212 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7489
StyI C'CwwG_G
Cuts at: 513 1065 3468 3873 3897 4928 513
Size: 552 2403 405 24 1031 3074
Fragments arranged by size:
3074 2403 1031 552 405 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4261 5516 5533 5686 5871 6316 325
Size: 1255 2681 1255 17 153 185 445 1498
Fragments arranged by size:
2681 1498 1255 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3362 4170 4310
Size: 274 80 33 51 1443 1385 808 140
Cuts at: 4310 5735 7447 96
Size: 1425 1712 138
Fragments arranged by size:
1712 1443 1425 1385 808 274 140 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3147 3179 3486 3518 7449 7481 3147
Size: 32 307 32 3931 32 3155
Fragments arranged by size:
3931 3155 307 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7489
XbaI T'CTAG_A
Cuts at: 4825 4825
Size: 7489
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3279 3279
Size: 7489
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7489
XmaI C'CCGG_G
Cuts at: 1870 2037 2105 4814 1870
Size: 167 68 2709 4545
Fragments arranged by size:
4545 2709 167 68
XmnI GAAnn'nnTTC
Cuts at: 3706 5618 3706
Size: 1912 5577
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5297 276
Size: 53 83 186 936 3763 2468
Fragments arranged by size:
3763 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FalI FseI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstZ17I EcoICRI EcoNI FspAI KasI
NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI