Restriction map for
pFN21AA1006
(Circular) (Six-base) MAPSORT of: pFN21AA1006.seq Check: 8990 from: 1 to: 8263
pFN21AA1006 8263 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6073 278
Size: 53 83 186 936 4537 2468
Fragments arranged by size:
4537 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4850 5606 1937
Size: 2913 756 4594
Fragments arranged by size:
4594 2913 756
Acc65I G'GTAC_C
Cuts at: 5553 5584 5553
Size: 31 8232
AclI AA'CG_TT
Cuts at: 1563 6390 6763 1563
Size: 4827 373 3063
Fragments arranged by size:
4827 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3665 6326 7710 7978 1632
Size: 2033 2661 1384 268 1917
Fragments arranged by size:
2661 2033 1917 1384 268
AfeI AGC'GCT
Cuts at: 4232 4232
Size: 8263
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8066
AflIII A'CryG_T
Cuts at: 1203 3521 7162 8139 1203
Size: 2318 3641 977 1327
Fragments arranged by size:
3641 2318 1327 977
AgeI A'CCGG_T
Cuts at: 7094 7094
Size: 8263
AhdI GACnn_n'nnGTC
Cuts at: 6992 6992
Size: 8263
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8263
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8263
AlwNI CAG_nnn'CTG
Cuts at: 3460 4980 6006 7578 3460
Size: 1520 1026 1572 4145
Fragments arranged by size:
4145 1572 1520 1026
ApaLI G'TGCA_C
Cuts at: 5418 6320 7476 5418
Size: 902 1156 6205
Fragments arranged by size:
6205 1156 902
ApoI r'AATT_y
Cuts at: 1493 2151 2459 3080 3116 3425 3629 4435 5145
Size: 658 308 621 36 309 204 806 710
Cuts at: 5145 5572 5798 5950 8059 1493
Size: 427 226 152 2109 1697
Fragments arranged by size:
2109 1697 806 710 658 621 427 309
308 226 204 152 36
AseI AT'TA_AT
Cuts at: 160 1032 4727 6817 160
Size: 872 3695 2090 1606
Fragments arranged by size:
3695 2090 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8263
AvaI C'yCGr_G
Cuts at: 1870 1943 2294 5588 1870
Size: 73 351 3294 4545
Fragments arranged by size:
4545 3294 351 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2688 2721 2688
Size: 33 8230
BamHI G'GATC_C
Cuts at: 4167 4756 5593 5960 4167
Size: 589 837 367 6470
Fragments arranged by size:
6470 837 589 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2375 5553 5584 7039 618
Size: 325 217 627 588 3178 31 1455 1842
Fragments arranged by size:
3178 1842 1455 627 588 325 217 31
BanII G_rGCy'C
Cuts at: 1391 5301 5582 1391
Size: 3910 281 4072
Fragments arranged by size:
4072 3910 281
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3019 3546 3848 4019 4363 4876 5027
Size: 971 1120 527 302 171 344 513 151
Cuts at: 5027 928
Size: 4164
Fragments arranged by size:
4164 1120 971 527 513 344 302 171
151
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6454 6488 1903
Size: 34 4517 34 3678
Fragments arranged by size:
4517 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3317 6155 7365 3317
Size: 2838 1210 4215
Fragments arranged by size:
4215 2838 1210
BclI T'GATC_A
Cuts at: 1548 2364 1548
Size: 816 7447
BfrBI ATG'CAT
Cuts at: 3034 3034
Size: 8263
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4985 6874 136
Size: 107 122 71 631 605 3313 1889 1525
Fragments arranged by size:
3313 1889 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3157 3739 8258 3157
Size: 582 4519 3162
Fragments arranged by size:
4519 3162 582
BlpI GC'TnA_GC
Cuts at: 3949 5680 3949
Size: 1731 6532
Bme1580I G_kGCm'C
Cuts at: 1790 5422 6324 7480 1790
Size: 3632 902 1156 2573
Fragments arranged by size:
3632 2573 1156 902
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8263
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4372 5994 6952
Size: 416 53 83 399 24 2955 1622 958
Cuts at: 6952 442
Size: 1753
Fragments arranged by size:
2955 1753 1622 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8263
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5267 5315 5267
Size: 48 8215
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3170 3849 4453 5412 6923 1239
Size: 1931 679 604 959 1511 2579
Fragments arranged by size:
2579 1931 1511 959 679 604
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4247 5237 6388 7253 7551 4247
Size: 990 1151 865 298 4959
Fragments arranged by size:
4959 1151 990 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2293 4089 6926 882
Size: 617 794 1796 2837 2219
Fragments arranged by size:
2837 2219 1796 794 617
BsaAI yAC'GTr
Cuts at: 493 1204 6054 493
Size: 711 4850 2702
Fragments arranged by size:
4850 2702 711
BsaBI GATnn'nnATC
Cuts at: 2778 8257 2778
Size: 5479 2784
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6070 6452 7087 275
Size: 53 83 186 936 4537 382 635 1451
Fragments arranged by size:
4537 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3271 6696 7094 7368 7515 7926 3271
Size: 3425 398 274 147 411 3608
Fragments arranged by size:
3608 3425 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2290 2320 3712 3742 4537
Size: 30 165 30 887 30 1392 30 795
Cuts at: 4537 4567 1178
Size: 30 4874
Fragments arranged by size:
4874 1392 887 795 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2041 3369 4077 1186
Size: 855 1328 708 5372
Fragments arranged by size:
5372 1328 855 708
BseYI C'CCAG_C
Cuts at: 3750 4344 7466 7962 3750
Size: 594 3122 496 4051
Fragments arranged by size:
4051 3122 594 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2358 2824 4255 5514 1163
Size: 573 622 466 1431 1259 3912
Fragments arranged by size:
3912 1431 1259 622 573 466
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5713 6474 7502 1540
Size: 275 186 3712 761 1028 2301
Fragments arranged by size:
3712 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3376 3785 5301 5422 6324 6409 7480 3376
Size: 409 1516 121 902 85 1071 4159
Fragments arranged by size:
4159 1516 1071 902 409 121 85
BsmI GAATG_Cn'
Cuts at: 3864 5774 5867 3864
Size: 1910 93 6260
Fragments arranged by size:
6260 1910 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5137 5137
Size: 8263
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3376 3785 5301 5422 5582 6324 6409
Size: 399 1586 409 1516 121 160 742 85
Cuts at: 6409 7480 1391
Size: 1071 2174
Fragments arranged by size:
2174 1586 1516 1071 742 409 399 160
121 85
BspEI T'CCGG_A
Cuts at: 3271 7926 3271
Size: 4655 3608
BspHI T'CATG_A
Cuts at: 2782 3226 5013 6152 7882 2782
Size: 444 1787 1139 1730 3163
Fragments arranged by size:
3163 1787 1730 1139 444
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2957 5618 844
Size: 2113 2661 3489
Fragments arranged by size:
3489 2661 2113
BsrBI CCG'CTC
Cuts at: 1394 1823 6150 1394
Size: 429 4327 3507
Fragments arranged by size:
4327 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3988 4107 6758 6932 65
Size: 1183 2740 119 2651 174 1396
Fragments arranged by size:
2740 2651 1396 1183 174 119
BsrFI r'CCGG_y
Cuts at: 1700 1810 6907 7094 1700
Size: 110 5097 187 2869
Fragments arranged by size:
5097 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2638 3462 96
Size: 2542 824 4897
Fragments arranged by size:
4897 2542 824
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8263
BssSI C'ACGA_G
Cuts at: 6323 7335 6323
Size: 1012 7251
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3257 4980 5426 794
Size: 2463 1723 446 3631
Fragments arranged by size:
3631 2463 1723 446
BstBI TT'CG_AA
Cuts at: 4369 7067 4369
Size: 2698 5565
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8263
BstXI CCAn_nnnn'nTGG
Cuts at: 2816 4262 2816
Size: 1446 6817
BstYI r'GATC_y
Cuts at: 1972 3157 3739 4167 4756 5593 5960 6345 6362
Size: 1185 582 428 589 837 367 385 17
Cuts at: 6362 7814 7900 7912 8258 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1185 837 589 582 428 385
367 346 86 17 12
BstZ17I GTA'TAC
Cuts at: 4851 4851
Size: 8263
BtgI C'CryG_G
Cuts at: 513 1065 3216 4079 513
Size: 552 2151 863 4697
Fragments arranged by size:
4697 2151 863 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7524
Fragments arranged by size:
7524 683 56
BtsI GCAGTG_nn'
Cuts at: 2068 2080 4267 4889 4981 5779 6573 6593 2068
Size: 12 2187 622 92 798 794 20 3738
Fragments arranged by size:
3738 2187 798 794 622 92 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8263
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3881 3916 4941 4976 529
Size: 35 3317 35 1025 35 3816
Fragments arranged by size:
3816 3317 1025 35 35 35
DraI TTT'AAA
Cuts at: 5567 5567
Size: 8263
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7270 809
Size: 507 5954 1802
Fragments arranged by size:
5954 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3341 3848 5710
Size: 54 1425 50 275 18 1511 507 1862
Cuts at: 5710 6599 8228 8
Size: 889 1629 43
Fragments arranged by size:
1862 1629 1511 1425 889 507 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5710 1537
Size: 275 3898 4090
Fragments arranged by size:
4090 3898 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5289 5460 6193 1353
Size: 3936 171 733 3423
Fragments arranged by size:
3936 3423 733 171
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6850 7224 7370 387
Size: 1064 138 5261 374 146 1280
Fragments arranged by size:
5261 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5299 5299
Size: 8263
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3170 3665 3849 4453 5412 6326 6923
Size: 393 1538 495 184 604 959 914 597
Cuts at: 6923 7710 7978 1239
Size: 787 268 1524
Fragments arranged by size:
1538 1524 959 914 787 604 597 495
393 268 184
EcoNI CCTnn'n_nnAGG
Cuts at: 5358 5358
Size: 8263
EcoO109I rG'GnC_Cy
Cuts at: 4349 4349
Size: 8263
EcoRI G'AATT_C
Cuts at: 5572 5950 5572
Size: 378 7885
EcoRV GAT'ATC
Cuts at: 3872 3872
Size: 8263
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2874 3899 4299 2874
Size: 1025 400 6838
Fragments arranged by size:
6838 1025 400
FspI TGC'GCA
Cuts at: 5343 6769 5343
Size: 1426 6837
HaeII r_GCGC'y
Cuts at: 1678 4234 5388 7410 1678
Size: 2556 1154 2022 2531
Fragments arranged by size:
2556 2531 2022 1154
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2568 2600 2705 2737 3919
Size: 32 135 32 996 32 105 32 1182
Cuts at: 3919 3951 6904 6936 6978 7010 1373
Size: 32 2953 32 42 32 2626
Fragments arranged by size:
2953 2626 1182 996 135 105 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5157 5607 5853 669
Size: 988 281 3219 450 246 3079
Fragments arranged by size:
3219 3079 988 450 281 246
HindIII A'AGCT_T
Cuts at: 748 3003 5310 748
Size: 2255 2307 3701
Fragments arranged by size:
3701 2307 2255
HpaI GTT'AAC
Cuts at: 5157 5853 5157
Size: 696 7567
KpnI G_GTAC'C
Cuts at: 5557 5588 5557
Size: 31 8232
MfeI C'AATT_G
Cuts at: 2601 2769 3893 5862 2601
Size: 168 1124 1969 5002
Fragments arranged by size:
5002 1969 1124 168
MluI A'CGCG_T
Cuts at: 8139 8139
Size: 8263
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2500 4304 4549 5028 7377 7561 1305
Size: 1195 1804 245 479 2349 184 2007
Fragments arranged by size:
2349 2007 1804 1195 479 245 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3343 10
Size: 54 1425 343 1511 4930
Fragments arranged by size:
4930 1511 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2331 2814 3411 3557 4260 5018 5517
Size: 648 1165 483 597 146 703 758 499
Cuts at: 5517 6221 6580 6739 518
Size: 704 359 159 2042
Fragments arranged by size:
2042 1165 758 704 703 648 597 499
483 359 159 146
MspA1I CmG'CkG
Cuts at: 1606 2342 5152 5679 6356 7504 7749 1606
Size: 736 2810 527 677 1148 245 2120
Fragments arranged by size:
2810 2120 1148 736 677 527 245
NcoI C'CATG_G
Cuts at: 513 1065 3216 513
Size: 552 2151 5560
Fragments arranged by size:
5560 2151 552
NdeI CA'TA_TG
Cuts at: 387 3613 3955 4741 387
Size: 3226 342 786 3909
Fragments arranged by size:
3909 3226 786 342
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8263
NotI GC'GGCC_GC
Cuts at: 5710 5710
Size: 8263
NsiI A_TGCA'T
Cuts at: 3036 3036
Size: 8263
NspI r_CATG'y
Cuts at: 1132 1229 3246 3525 4129 4992 5197 5516 5621
Size: 97 2017 279 604 863 205 319 105
Cuts at: 5621 7166 1132
Size: 1545 2229
Fragments arranged by size:
2229 2017 1545 863 604 319 279 205
105 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8263
PciI A'CATG_T
Cuts at: 3521 7162 3521
Size: 3641 4622
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3350 1108
Size: 2242 6021
PfoI T'CCnGG_A
Cuts at: 3865 4351 5353 3865
Size: 486 1002 6775
Fragments arranged by size:
6775 1002 486
PmeI GTTT'AAAC
Cuts at: 5567 5567
Size: 8263
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4282 4314 6332 6364 7845 7877 4282
Size: 32 2018 32 1481 32 4668
Fragments arranged by size:
4668 2018 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4349 4349
Size: 8263
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8263
PsiI TTA'TAA
Cuts at: 3627 5833 3627
Size: 2206 6057
PstI C_TGCA'G
Cuts at: 830 2134 3456 5479 5615 830
Size: 1304 1322 2023 136 3478
Fragments arranged by size:
3478 2023 1322 1304 136
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8263
PvuII CAG'CTG
Cuts at: 2342 2342
Size: 8263
SacI G_AGCT'C
Cuts at: 5301 5301
Size: 8263
SalI G'TCGA_C
Cuts at: 1936 5605 1936
Size: 3669 4594
SapI GCTCTTCn'nnn_
Cuts at: 5289 5289
Size: 8263
SbfI CC_TGCA'GG
Cuts at: 5615 5615
Size: 8263
ScaI AGT'ACT
Cuts at: 3054 3288 4020 6511 8223 3054
Size: 234 732 2491 1712 3094
Fragments arranged by size:
3094 2491 1712 732 234
SexAI A'CCwGG_T
Cuts at: 2302 5359 2302
Size: 3057 5206
SfcI C'TryA_G
Cuts at: 826 1045 2130 2348 2700 3297 3452 5186 5475
Size: 219 1085 218 352 597 155 1734 289
Cuts at: 5475 5611 6746 7427 7618 826
Size: 136 1135 681 191 1471
Fragments arranged by size:
1734 1471 1135 1085 681 597 352 289
219 218 191 155 136
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8263
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8263
SmaI CCC'GGG
Cuts at: 1872 5590 1872
Size: 3718 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4226 5252 6367 7268 7530 820
Size: 197 926 2283 1026 1115 901 262 1553
Fragments arranged by size:
2283 1553 1115 1026 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6054 493
Size: 5561 2702
SpeI A'CTAG_T
Cuts at: 152 3932 152
Size: 3780 4483
SphI G_CATG'C
Cuts at: 1132 4129 4992 5621 1132
Size: 2997 863 629 3774
Fragments arranged by size:
3774 2997 863 629
SspI AAT'ATT
Cuts at: 5 52 2554 2976 3588 6187 5
Size: 47 2502 422 612 2599 2081
Fragments arranged by size:
2599 2502 2081 612 422 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8263
StyI C'CwwG_G
Cuts at: 513 1065 2379 3216 3367 5702 513
Size: 552 1314 837 151 2335 3074
Fragments arranged by size:
3074 2335 1314 837 552 151
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4709 4728 6290 6307 6460 6645 7090
Size: 1255 3129 19 1562 17 153 185 445
Cuts at: 7090 325
Size: 1498
Fragments arranged by size:
3129 1562 1498 1255 445 185 153 19
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2638 3052 3286
Size: 274 80 33 51 1443 661 414 234
Cuts at: 3286 3462 4018 6509 8221 96
Size: 176 556 2491 1712 138
Fragments arranged by size:
2491 1712 1443 661 556 414 274 234
176 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2290 2322 8223 8255 2290
Size: 32 5901 32 2298
Fragments arranged by size:
5901 2298 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8263
XbaI T'CTAG_A
Cuts at: 4451 5599 4451
Size: 1148 7115
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2330 4582 4746 2330
Size: 2252 164 5847
Fragments arranged by size:
5847 2252 164
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8263
XmaI C'CCGG_G
Cuts at: 1870 5588 1870
Size: 3718 4545
XmnI GAAnn'nnTTC
Cuts at: 2107 6392 2107
Size: 4285 3978
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6071 276
Size: 53 83 186 936 4537 2468
Fragments arranged by size:
4537 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BbvCI Bpu10I
BsiWI Bsu36I DraIII FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SfiI SfoI SrfI SwaI