Restriction map for
pFN21AA0996
(Circular) (Six-base) MAPSORT of: pFN21AA0996.seq Check: 1667 from: 1 to: 7249
pFN21AA0996 7249 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4402 4402
Size: 7249
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2097 5059 278
Size: 53 83 186 936 561 2962 2468
Fragments arranged by size:
2962 2468 936 561 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2058 4592 1937
Size: 121 2534 4594
Fragments arranged by size:
4594 2534 121
Acc65I G'GTAC_C
Cuts at: 4570 4570
Size: 7249
AclI AA'CG_TT
Cuts at: 1563 5376 5749 1563
Size: 3813 373 3063
Fragments arranged by size:
3813 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2378 2691 3771 4524 5312 6696 6964 1632
Size: 746 313 1080 753 788 1384 268 1917
Fragments arranged by size:
1917 1384 1080 788 753 746 313 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7052
AflIII A'CryG_T
Cuts at: 1203 6148 7125 1203
Size: 4945 977 1327
Fragments arranged by size:
4945 1327 977
AgeI A'CCGG_T
Cuts at: 6080 6080
Size: 7249
AhdI GACnn_n'nnGTC
Cuts at: 5978 5978
Size: 7249
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7249
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2504 1184
Size: 1320 5929
AlwNI CAG_nnn'CTG
Cuts at: 2569 4992 6564 2569
Size: 2423 1572 3254
Fragments arranged by size:
3254 2423 1572
ApaI G_GGCC'C
Cuts at: 2091 2166 2091
Size: 75 7174
ApaLI G'TGCA_C
Cuts at: 5306 6462 5306
Size: 1156 6093
ApoI r'AATT_y
Cuts at: 1493 2677 2936 2959 3726 3734 4558 4784 4936
Size: 1184 259 23 767 8 824 226 152
Cuts at: 4936 7045 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1184 824 767 259 226 152
23 8
AseI AT'TA_AT
Cuts at: 160 1032 3621 5803 160
Size: 872 2589 2182 1606
Fragments arranged by size:
2589 2182 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7249
AvaI C'yCGr_G
Cuts at: 1870 1943 3132 4574 1870
Size: 73 1189 1442 4545
Fragments arranged by size:
4545 1442 1189 73
BamHI G'GATC_C
Cuts at: 4579 4946 4579
Size: 367 6882
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4570 6025 618
Size: 325 217 627 2783 1455 1842
Fragments arranged by size:
2783 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2091 2166 4568 1391
Size: 700 75 2402 4072
Fragments arranged by size:
4072 2402 700 75
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2316 2850 3063 3954 4323 928
Size: 971 417 534 213 891 369 3854
Fragments arranged by size:
3854 971 891 534 417 369 213
BbvCI CC'TCA_GC
Cuts at: 2013 2013
Size: 7249
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3420 3454 5440 5474 1903
Size: 34 50 34 1399 34 1986 34 3678
Fragments arranged by size:
3678 1986 1399 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3808 5141 6351 3808
Size: 1333 1210 4706
Fragments arranged by size:
4706 1333 1210
BclI T'GATC_A
Cuts at: 1548 3649 1548
Size: 2101 5148
BfrBI ATG'CAT
Cuts at: 3523 3523
Size: 7249
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2132 5860 136
Size: 107 122 71 631 605 460 3728 1525
Fragments arranged by size:
3728 1525 631 605 460 122 107 71
BglII A'GATC_T
Cuts at: 7244 7244
Size: 7249
BlpI GC'TnA_GC
Cuts at: 2219 4666 2219
Size: 2447 4802
Bme1580I G_kGCm'C
Cuts at: 1790 2091 2166 2324 5310 6466 1790
Size: 301 75 158 2986 1156 2573
Fragments arranged by size:
2986 2573 1156 301 158 75
BmgBI CAC'GTC
Cuts at: 1318 2233 2260 1318
Size: 915 27 6307
Fragments arranged by size:
6307 915 27
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4330 4980 5938
Size: 416 53 83 399 24 2913 650 958
Cuts at: 5938 442
Size: 1753
Fragments arranged by size:
2913 1753 958 650 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7249
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2216 2459 3263 3849 5909 1239
Size: 977 243 804 586 2060 2579
Fragments arranged by size:
2579 2060 977 804 586 243
Bpu10I CC'TnA_GC
Cuts at: 2013 2456 2013
Size: 443 6806
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2507 5374 6239 6537 2507
Size: 2867 865 298 3219
Fragments arranged by size:
3219 2867 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5912 882
Size: 617 4413 2219
Fragments arranged by size:
4413 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3635 5040 493
Size: 711 2431 1405 2702
Fragments arranged by size:
2702 2431 1405 711
BsaBI GATnn'nnATC
Cuts at: 4265 7243 4265
Size: 2978 4271
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2094 5056 5438 6073
Size: 53 83 186 936 561 2962 382 635
Cuts at: 6073 275
Size: 1451
Fragments arranged by size:
2962 1451 936 635 561 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2721 5682 6080 6354 6501 6912 2721
Size: 2961 398 274 147 411 3058
Fragments arranged by size:
3058 2961 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3964 3994 4158 4188 1178
Size: 30 165 30 2561 30 164 30 4239
Fragments arranged by size:
4239 2561 165 164 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2456 3541 3782 4010 4013 1186
Size: 1270 1085 241 228 3 4422
Fragments arranged by size:
4422 1270 1085 241 228 3
BseYI C'CCAG_C
Cuts at: 2147 2562 3376 6452 6948 2147
Size: 415 814 3076 496 2448
Fragments arranged by size:
3076 2448 814 496 415
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2384 2414 2766 2817 3368 3817 1163
Size: 573 648 30 352 51 551 449 4595
Fragments arranged by size:
4595 648 573 551 449 352 51 30
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4699 5460 6488 1540
Size: 275 186 2698 761 1028 2301
Fragments arranged by size:
2698 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5310 5395 6466 5310
Size: 85 1071 6093
Fragments arranged by size:
6093 1071 85
BsmI GAATG_Cn'
Cuts at: 3376 3431 4760 4853 3376
Size: 55 1329 93 5772
Fragments arranged by size:
5772 1329 93 55
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2166 2324 4568 5310 5395 6466
Size: 399 301 75 158 2244 742 85 1071
Cuts at: 6466 1391
Size: 2174
Fragments arranged by size:
2244 2174 1071 742 399 301 158 85
75
BspEI T'CCGG_A
Cuts at: 2721 6912 2721
Size: 4191 3058
BspHI T'CATG_A
Cuts at: 2283 5138 6868 2283
Size: 2855 1730 2664
Fragments arranged by size:
2855 2664 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4402 4604 844
Size: 3558 202 3489
Fragments arranged by size:
3558 3489 202
BsrBI CCG'CTC
Cuts at: 1394 1823 2068 2446 5136 1394
Size: 429 245 378 2690 3507
Fragments arranged by size:
3507 2690 429 378 245
BsrDI GCAATG_nn'
Cuts at: 65 1248 3241 5744 5918 65
Size: 1183 1993 2503 174 1396
Fragments arranged by size:
2503 1993 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5893 6080 1700
Size: 110 4083 187 2869
Fragments arranged by size:
4083 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7249
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7249
BssSI C'ACGA_G
Cuts at: 3044 5309 6321 3044
Size: 2265 1012 3972
Fragments arranged by size:
3972 2265 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2789 794
Size: 1995 5254
BstBI TT'CG_AA
Cuts at: 2954 3202 6053 2954
Size: 248 2851 4150
Fragments arranged by size:
4150 2851 248
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7249
BstXI CCAn_nnnn'nTGG
Cuts at: 2233 2233
Size: 7249
BstYI r'GATC_y
Cuts at: 1972 3943 4579 4946 5331 5348 6800 6886 6898
Size: 1971 636 367 385 17 1452 86 12
Cuts at: 6898 7244 1972
Size: 346 1977
Fragments arranged by size:
1977 1971 1452 636 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 4452 4452
Size: 7249
BtgI C'CryG_G
Cuts at: 513 1065 2115 2130 2189 513
Size: 552 1050 15 59 5573
Fragments arranged by size:
5573 1050 552 59 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4199 487
Size: 683 56 2973 3537
Fragments arranged by size:
3537 2973 683 56
BtsI GCAGTG_nn'
Cuts at: 2326 2461 3805 4765 5559 5579 2326
Size: 135 1344 960 794 20 3996
Fragments arranged by size:
3996 1344 960 794 135 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7249
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7214
DraI TTT'AAA
Cuts at: 2941 3052 4553 2941
Size: 111 1501 5637
Fragments arranged by size:
5637 1501 111
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6256 809
Size: 507 4940 1802
Fragments arranged by size:
4940 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2103 4696 5585
Size: 54 1425 50 275 18 273 2593 889
Cuts at: 5585 7214 8
Size: 1629 43
Fragments arranged by size:
2593 1629 1425 889 275 273 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4696 1537
Size: 275 2884 4090
Fragments arranged by size:
4090 2884 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2523 2742 3255 3536 3790 4338 5179 1353
Size: 1170 219 513 281 254 548 841 3423
Fragments arranged by size:
3423 1170 841 548 513 281 254 219
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5836 6210 6356 387
Size: 1064 138 4247 374 146 1280
Fragments arranged by size:
4247 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2216 2378 2459 2691 3263 3771 3849
Size: 393 584 162 81 232 572 508 78
Cuts at: 3849 4524 5312 5909 6696 6964 1239
Size: 675 788 597 787 268 1524
Fragments arranged by size:
1524 788 787 675 597 584 572 508
393 268 232 162 81 78
EcoNI CCTnn'n_nnAGG
Cuts at: 3560 3560
Size: 7249
EcoO109I rG'GnC_Cy
Cuts at: 2087 2162 2163 2087
Size: 75 1 7173
Fragments arranged by size:
7173 75 1
EcoRI G'AATT_C
Cuts at: 2959 3734 4558 4936 2959
Size: 775 824 378 5272
Fragments arranged by size:
5272 824 775 378
EcoRV GAT'ATC
Cuts at: 4350 4350
Size: 7249
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3552 4096 3552
Size: 544 6705
FspI TGC'GCA
Cuts at: 5755 5755
Size: 7249
HaeII r_GCGC'y
Cuts at: 1678 2226 6396 1678
Size: 548 4170 2531
Fragments arranged by size:
4170 2531 548
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3205 3237 3289 3321 3893
Size: 32 135 32 1633 32 52 32 572
Cuts at: 3893 3925 4316 4348 5890 5922 5964 5996 1373
Size: 32 391 32 1542 32 42 32 2626
Fragments arranged by size:
2626 1633 1542 572 391 135 52 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4593 4839 669
Size: 988 281 2655 246 3079
Fragments arranged by size:
3079 2655 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2634 3534 748
Size: 1886 900 4463
Fragments arranged by size:
4463 1886 900
HpaI GTT'AAC
Cuts at: 4839 4839
Size: 7249
KpnI G_GTAC'C
Cuts at: 4574 4574
Size: 7249
MfeI C'AATT_G
Cuts at: 4848 4848
Size: 7249
MluI A'CGCG_T
Cuts at: 7125 7125
Size: 7249
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3042 6363 6547 1305
Size: 1737 3321 184 2007
Fragments arranged by size:
3321 2007 1737 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5427
Fragments arranged by size:
5427 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2231 2610 2793 5207 5566 5725 518
Size: 648 1065 379 183 2414 359 159 2042
Fragments arranged by size:
2414 2042 1065 648 379 359 183 159
MspA1I CmG'CkG
Cuts at: 1606 2021 2076 2117 2191 2428 2569 3756 4665
Size: 415 55 41 74 237 141 1187 909
Cuts at: 4665 5342 6490 6735 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1187 1148 909 677 415 245 237
141 74 55 41
NcoI C'CATG_G
Cuts at: 513 1065 2130 513
Size: 552 1065 5632
Fragments arranged by size:
5632 1065 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7249
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7249
NotI GC'GGCC_GC
Cuts at: 4696 4696
Size: 7249
NsiI A_TGCA'T
Cuts at: 3525 3525
Size: 7249
NspI r_CATG'y
Cuts at: 1132 1229 2041 2057 4607 6152 1132
Size: 97 812 16 2550 1545 2229
Fragments arranged by size:
2550 2229 1545 812 97 16
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7249
PciI A'CATG_T
Cuts at: 6148 6148
Size: 7249
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4241 1108
Size: 3133 4116
PfoI T'CCnGG_A
Cuts at: 3279 3279
Size: 7249
PmeI GTTT'AAAC
Cuts at: 4553 4553
Size: 7249
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5318 5350 6831 6863 5318
Size: 32 1481 32 5704
Fragments arranged by size:
5704 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7249
PsiI TTA'TAA
Cuts at: 3302 4819 3302
Size: 1517 5732
PspOMI G'GGCC_C
Cuts at: 2087 2162 2087
Size: 75 7174
PspXI vC'TCGA_Gb
Cuts at: 3132 3132
Size: 7249
PstI C_TGCA'G
Cuts at: 830 2274 2757 4601 830
Size: 1444 483 1844 3478
Fragments arranged by size:
3478 1844 1444 483
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7249
PvuII CAG'CTG
Cuts at: 2428 2569 3756 2428
Size: 141 1187 5921
Fragments arranged by size:
5921 1187 141
SacII CC_GC'GG
Cuts at: 2118 2192 2118
Size: 74 7175
SalI G'TCGA_C
Cuts at: 1936 4591 1936
Size: 2655 4594
SapI GCTCTTCn'nnn_
Cuts at: 2742 3255 2742
Size: 513 6736
SbfI CC_TGCA'GG
Cuts at: 4601 4601
Size: 7249
ScaI AGT'ACT
Cuts at: 2393 3666 5497 7209 2393
Size: 1273 1831 1712 2433
Fragments arranged by size:
2433 1831 1712 1273
SexAI A'CCwGG_T
Cuts at: 2762 2762
Size: 7249
SfcI C'TryA_G
Cuts at: 826 1045 2270 2753 4597 5732 6413 6604 826
Size: 219 1225 483 1844 1135 681 191 1471
Fragments arranged by size:
1844 1471 1225 1135 681 483 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2132 2132
Size: 7249
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7249
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7249
SmaI CCC'GGG
Cuts at: 1872 4576 1872
Size: 2704 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2522 3132 5353 6254 6516 820
Size: 197 926 579 610 2221 901 262 1553
Fragments arranged by size:
2221 1553 926 901 610 579 262 197
SnaBI TAC'GTA
Cuts at: 493 5040 493
Size: 4547 2702
SpeI A'CTAG_T
Cuts at: 152 2893 152
Size: 2741 4508
SphI G_CATG'C
Cuts at: 1132 2041 4607 1132
Size: 909 2566 3774
Fragments arranged by size:
3774 2566 909
SspI AAT'ATT
Cuts at: 5 52 5173 5
Size: 47 5121 2081
Fragments arranged by size:
5121 2081 47
StuI AGG'CCT
Cuts at: 1517 2621 1517
Size: 1104 6145
StyI C'CwwG_G
Cuts at: 513 1065 2130 2811 3612 4688 513
Size: 552 1065 681 801 1076 3074
Fragments arranged by size:
3074 1076 1065 801 681 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2576 4133 5276 5293 5446 5631 6076
Size: 1255 996 1557 1143 17 153 185 445
Cuts at: 6076 325
Size: 1498
Fragments arranged by size:
1557 1498 1255 1143 996 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2391 3664 5495
Size: 274 80 33 51 1443 414 1273 1831
Cuts at: 5495 7207 96
Size: 1712 138
Fragments arranged by size:
1831 1712 1443 1273 414 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2397 2429 7209 7241 2397
Size: 32 4780 32 2405
Fragments arranged by size:
4780 2405 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7249
XbaI T'CTAG_A
Cuts at: 4585 4585
Size: 7249
XhoI C'TCGA_G
Cuts at: 1943 3132 1943
Size: 1189 6060
XmaI C'CCGG_G
Cuts at: 1870 4574 1870
Size: 2704 4545
XmnI GAAnn'nnTTC
Cuts at: 3961 4473 5378 3961
Size: 512 905 5832
Fragments arranged by size:
5832 905 512
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2095 5057 276
Size: 53 83 186 936 561 2962 2468
Fragments arranged by size:
2962 2468 936 561 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI
BsmBI BstZ17I DraIII EcoICRI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PpuMI PsrI RsrII
SacI SanDI SfoI SrfI SwaI XcmI