Restriction map for
pFN21AA0994
(Circular) (Six-base) MAPSORT of: pFN21AA0994.seq Check: 5531 from: 1 to: 6700
pFN21AA0994 6700 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3358 3358
Size: 6700
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4510 278
Size: 53 83 186 936 2974 2468
Fragments arranged by size:
2974 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2091 2520 4043 1937
Size: 154 429 1523 4594
Fragments arranged by size:
4594 1523 429 154
Acc65I G'GTAC_C
Cuts at: 4021 4021
Size: 6700
AclI AA'CG_TT
Cuts at: 1563 4827 5200 1563
Size: 3264 373 3063
Fragments arranged by size:
3264 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2107 2150 3029 3377 4763 6147 6415 1632
Size: 475 43 879 348 1386 1384 268 1917
Fragments arranged by size:
1917 1386 1384 879 475 348 268 43
AfeI AGC'GCT
Cuts at: 2207 3641 2207
Size: 1434 5266
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6503
AflIII A'CryG_T
Cuts at: 1203 2143 2145 2544 5599 6576 1203
Size: 940 2 399 3055 977 1327
Fragments arranged by size:
3055 1327 977 940 399 2
AgeI A'CCGG_T
Cuts at: 5531 5531
Size: 6700
AhdI GACnn_n'nnGTC
Cuts at: 5429 5429
Size: 6700
AleI CACnn'nnGTG
Cuts at: 1166 3858 1166
Size: 2692 4008
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2249 1184
Size: 1065 5635
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2648 2680 2735 2767 2648
Size: 32 55 32 6581
Fragments arranged by size:
6581 55 32 32
AlwNI CAG_nnn'CTG
Cuts at: 4443 6015 4443
Size: 1572 5128
ApaI G_GGCC'C
Cuts at: 2116 2435 3167 2116
Size: 319 732 5649
Fragments arranged by size:
5649 732 319
ApaLI G'TGCA_C
Cuts at: 4757 5913 4757
Size: 1156 5544
ApoI r'AATT_y
Cuts at: 1493 3461 3923 4009 4235 4387 6496 1493
Size: 1968 462 86 226 152 2109 1697
Fragments arranged by size:
2109 1968 1697 462 226 152 86
AseI AT'TA_AT
Cuts at: 160 1032 5254 160
Size: 872 4222 1606
Fragments arranged by size:
4222 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6700
AvaI C'yCGr_G
Cuts at: 1870 1943 2435 3120 3186 4025 1870
Size: 73 492 685 66 839 4545
Fragments arranged by size:
4545 839 685 492 73 66
BamHI G'GATC_C
Cuts at: 3048 4030 4397 3048
Size: 982 367 5351
Fragments arranged by size:
5351 982 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2069 3376 3947 3994 4021
Size: 325 217 627 282 1307 571 47 27
Cuts at: 4021 5476 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1307 627 571 325 282 217
47 27
BanII G_rGCy'C
Cuts at: 1391 2116 2337 2435 3159 3167 3195 3390 3876
Size: 725 221 98 724 8 28 195 486
Cuts at: 3876 4019 1391
Size: 143 4072
Fragments arranged by size:
4072 725 724 486 221 195 143 98
28 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5729
BbvCI CC'TCA_GC
Cuts at: 2178 2178
Size: 6700
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2302 2336 3277 3311 3880
Size: 34 50 34 281 34 941 34 569
Cuts at: 3880 3893 3914 3927 4891 4925 1903
Size: 13 21 13 964 34 3678
Fragments arranged by size:
3678 964 941 569 281 50 34 34
34 34 34 21 13 13
BciVI GTATCCnnnnn_n'
Cuts at: 2588 4592 5802 2588
Size: 2004 1210 3486
Fragments arranged by size:
3486 2004 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6700
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2439 3817 5311
Size: 107 122 71 631 605 767 1378 1494
Cuts at: 5311 136
Size: 1525
Fragments arranged by size:
1525 1494 1378 767 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2355 2533 6695 2355
Size: 178 4162 2360
Fragments arranged by size:
4162 2360 178
BlpI GC'TnA_GC
Cuts at: 4117 4117
Size: 6700
Bme1580I G_kGCm'C
Cuts at: 1790 2072 2116 2435 3167 3999 4761 5917 1790
Size: 282 44 319 732 832 762 1156 2573
Fragments arranged by size:
2573 1156 832 762 732 319 282 44
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6700
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3245 4431 5389
Size: 416 53 83 399 24 1828 1186 958
Cuts at: 5389 442
Size: 1753
Fragments arranged by size:
1828 1753 1186 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6700
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2806 3409 2806
Size: 603 6097
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2843 3257 3866 3890 5360 1239
Size: 1604 414 609 24 1470 2579
Fragments arranged by size:
2579 1604 1470 609 414 24
Bpu10I CC'TnA_GC
Cuts at: 2178 2178
Size: 6700
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2522 3653 4825 5690 5988 2522
Size: 1131 1172 865 298 3234
Fragments arranged by size:
3234 1172 1131 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2806 5363 882
Size: 617 1307 2557 2219
Fragments arranged by size:
2557 2219 1307 617
BsaAI yAC'GTr
Cuts at: 493 1204 2062 2146 2653 3181 3418 3856 4491
Size: 711 858 84 507 528 237 438 635
Cuts at: 4491 493
Size: 2702
Fragments arranged by size:
2702 858 711 635 528 507 438 237
84
BsaBI GATnn'nnATC
Cuts at: 6694 6694
Size: 6700
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4507 4889 5524 275
Size: 53 83 186 936 2974 382 635 1451
Fragments arranged by size:
2974 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2880 5133 5531 5805 5952 6363 2880
Size: 2253 398 274 147 411 3217
Fragments arranged by size:
3217 2253 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2042 2072 2648 2678 2735
Size: 30 165 30 639 30 576 30 57
Cuts at: 2735 2765 3614 3644 1178
Size: 30 849 30 4234
Fragments arranged by size:
4234 849 639 576 165 57 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2195 3845 1186
Size: 1009 1650 4041
Fragments arranged by size:
4041 1650 1009
BseYI C'CCAG_C
Cuts at: 2065 2104 2222 3363 3679 5903 6399 2065
Size: 39 118 1141 316 2224 496 2366
Fragments arranged by size:
2366 2224 1141 496 316 118 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3392 3638 1163
Size: 573 1656 246 4225
Fragments arranged by size:
4225 1656 573 246
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2581 3043 4150 4911 5939 1540
Size: 275 186 580 462 1107 761 1028 2301
Fragments arranged by size:
2301 1107 1028 761 580 462 275 186
BsiHKAI G_wGCw'C
Cuts at: 2261 3159 3390 4761 4846 5917 2261
Size: 898 231 1371 85 1071 3044
Fragments arranged by size:
3044 1371 1071 898 231 85
BsmI GAATG_Cn'
Cuts at: 2313 3842 3893 4211 4304 2313
Size: 1529 51 318 93 4709
Fragments arranged by size:
4709 1529 318 93 51
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2116 2261 2337 2435 3159 3167
Size: 399 282 44 145 76 98 724 8
Cuts at: 3167 3195 3390 3876 3999 4019 4761 4846 5917
Size: 28 195 486 123 20 742 85 1071
Cuts at: 5917 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 724 486 399 282 195
145 123 98 85 76 44 28 20
8
BspEI T'CCGG_A
Cuts at: 2880 6363 2880
Size: 3483 3217
BspHI T'CATG_A
Cuts at: 2898 4589 6319 2898
Size: 1691 1730 3279
Fragments arranged by size:
3279 1730 1691
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3358 3717 4055 844
Size: 2514 359 338 3489
Fragments arranged by size:
3489 2514 359 338
BsrBI CCG'CTC
Cuts at: 1394 1823 2017 3716 4587 1394
Size: 429 194 1699 871 3507
Fragments arranged by size:
3507 1699 871 429 194
BsrDI GCAATG_nn'
Cuts at: 65 1248 5195 5369 65
Size: 1183 3947 174 1396
Fragments arranged by size:
3947 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2697 5344 5531 1700
Size: 110 887 2647 187 2869
Fragments arranged by size:
2869 2647 887 187 110
BsrGI T'GTAC_A
Cuts at: 96 2610 96
Size: 2514 4186
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6700
BssSI C'ACGA_G
Cuts at: 4760 5772 4760
Size: 1012 5688
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3994 794
Size: 3200 3500
BstBI TT'CG_AA
Cuts at: 5504 5504
Size: 6700
BstEII G'GTnAC_C
Cuts at: 1184 2239 2281 1184
Size: 1055 42 5603
Fragments arranged by size:
5603 1055 42
BstXI CCAn_nnnn'nTGG
Cuts at: 2458 2458
Size: 6700
BstYI r'GATC_y
Cuts at: 1972 2355 2533 3048 4030 4397 4782 4799 6251
Size: 383 178 515 982 367 385 17 1452
Cuts at: 6251 6337 6349 6695 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 982 515 385 383 367 346
178 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2739 2891 3102 3381 3529 513
Size: 552 1674 152 211 279 148 3684
Fragments arranged by size:
3684 1674 552 279 211 152 148
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5961
Fragments arranged by size:
5961 683 56
BtsI GCAGTG_nn'
Cuts at: 2278 4216 5010 5030 2278
Size: 1938 794 20 3948
Fragments arranged by size:
3948 1938 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6700
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6665
DraI TTT'AAA
Cuts at: 4004 4004
Size: 6700
DraIII CAC_nnn'GTG
Cuts at: 3994 3994
Size: 6700
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5707 809
Size: 507 4391 1802
Fragments arranged by size:
4391 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2187 2671 3283
Size: 54 1425 50 275 18 357 484 612
Cuts at: 3283 3477 3526 4147 5036 6665 8
Size: 194 49 621 889 1629 43
Fragments arranged by size:
1629 1425 889 621 612 484 357 275
194 54 50 49 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4147 1537
Size: 275 2335 4090
Fragments arranged by size:
4090 2335 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2342 3509 4630 1353
Size: 989 1167 1121 3423
Fragments arranged by size:
3423 1167 1121 989
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2716 5287 5661 5807 387
Size: 1064 138 1127 2571 374 146 1280
Fragments arranged by size:
2571 1280 1127 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3157 3388 3157
Size: 231 6469
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2107 2150 2843 3029 3257 3377 3866
Size: 393 475 43 693 186 228 120 489
Cuts at: 3866 3890 4763 5360 6147 6415 1239
Size: 24 873 597 787 268 1524
Fragments arranged by size:
1524 873 787 693 597 489 475 393
268 228 186 120 43 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2969 3617 3671 2969
Size: 648 54 5998
Fragments arranged by size:
5998 648 54
EcoO109I rG'GnC_Cy
Cuts at: 2112 2431 2432 2573 3082 3793 2112
Size: 319 1 141 509 711 5019
Fragments arranged by size:
5019 711 509 319 141 1
EcoRI G'AATT_C
Cuts at: 4009 4387 4009
Size: 378 6322
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3034 3034
Size: 6700
FspI TGC'GCA
Cuts at: 5206 5206
Size: 6700
HaeII r_GCGC'y
Cuts at: 1678 2209 3643 5847 1678
Size: 531 1434 2204 2531
Fragments arranged by size:
2531 2204 1434 531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3416 3448 3525 3557 5341
Size: 32 135 32 1844 32 77 32 1784
Cuts at: 5341 5373 5415 5447 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1844 1784 135 77 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2290 4044 4290 669
Size: 988 281 352 1754 246 3079
Fragments arranged by size:
3079 1754 988 352 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6700
HpaI GTT'AAC
Cuts at: 4290 4290
Size: 6700
KpnI G_GTAC'C
Cuts at: 4025 4025
Size: 6700
MfeI C'AATT_G
Cuts at: 4299 4299
Size: 6700
MluI A'CGCG_T
Cuts at: 6576 6576
Size: 6700
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3026 3305 3627 5814 5998 1305
Size: 1721 279 322 2187 184 2007
Fragments arranged by size:
2187 2007 1721 322 279 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2189 3479 10
Size: 54 1425 343 357 1290 3231
Fragments arranged by size:
3231 1425 1290 357 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2618 3420 3779 3858 4658 5017 5176
Size: 648 1452 802 359 79 800 359 159
Cuts at: 5176 518
Size: 2042
Fragments arranged by size:
2042 1452 802 800 648 359 359 159
79
MspA1I CmG'CkG
Cuts at: 1606 2741 4116 4793 5941 6186 1606
Size: 1135 1375 677 1148 245 2120
Fragments arranged by size:
2120 1375 1148 1135 677 245
NaeI GCC'GGC
Cuts at: 2699 2699
Size: 6700
NcoI C'CATG_G
Cuts at: 513 1065 3529 513
Size: 552 2464 3684
Fragments arranged by size:
3684 2464 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6700
NgoMIV G'CCGG_C
Cuts at: 2697 2697
Size: 6700
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6700
NotI GC'GGCC_GC
Cuts at: 4147 4147
Size: 6700
NspI r_CATG'y
Cuts at: 1132 1229 2548 4058 5603 1132
Size: 97 1319 1510 1545 2229
Fragments arranged by size:
2229 1545 1510 1319 97
PasI CC'CwG_GG
Cuts at: 1348 3877 1348
Size: 2529 4171
PciI A'CATG_T
Cuts at: 2544 5599 2544
Size: 3055 3645
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2692 3173 1108
Size: 1584 481 4635
Fragments arranged by size:
4635 1584 481
PfoI T'CCnGG_A
Cuts at: 2420 3051 3867 2420
Size: 631 816 5253
Fragments arranged by size:
5253 816 631
PmeI GTTT'AAAC
Cuts at: 4004 4004
Size: 6700
PmlI CAC'GTG
Cuts at: 2146 3418 3856 2146
Size: 1272 438 4990
Fragments arranged by size:
4990 1272 438
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2648 2680 2735 2767 4769 4801 6282 6314 2648
Size: 32 55 32 2002 32 1481 32 3034
Fragments arranged by size:
3034 2002 1481 55 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3793 3793
Size: 6700
PshAI GACnn'nnGTC
Cuts at: 1613 2087 1613
Size: 474 6226
PsiI TTA'TAA
Cuts at: 4270 4270
Size: 6700
PspOMI G'GGCC_C
Cuts at: 2112 2431 3163 2112
Size: 319 732 5649
Fragments arranged by size:
5649 732 319
PstI C_TGCA'G
Cuts at: 830 2034 4052 830
Size: 1204 2018 3478
Fragments arranged by size:
3478 2018 1204
PvuI CG_AT'CG
Cuts at: 2001 3043 2001
Size: 1042 5658
RsrII CG'GwC_CG
Cuts at: 2019 2019
Size: 6700
SacI G_AGCT'C
Cuts at: 3159 3390 3159
Size: 231 6469
SacII CC_GC'GG
Cuts at: 2742 2742
Size: 6700
SalI G'TCGA_C
Cuts at: 1936 4042 1936
Size: 2106 4594
SapI GCTCTTCn'nnn_
Cuts at: 2342 3509 2342
Size: 1167 5533
SbfI CC_TGCA'GG
Cuts at: 4052 4052
Size: 6700
ScaI AGT'ACT
Cuts at: 4948 6660 4948
Size: 1712 4988
SfcI C'TryA_G
Cuts at: 826 1045 2030 2092 2521 4048 5183 5864 6055
Size: 219 985 62 429 1527 1135 681 191
Cuts at: 6055 826
Size: 1471
Fragments arranged by size:
1527 1471 1135 985 681 429 219 191
62
SfiI GGCCn_nnn'nGGCC
Cuts at: 2439 2439
Size: 6700
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6700
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6700
SmaI CCC'GGG
Cuts at: 1872 3188 4027 1872
Size: 1316 839 4545
Fragments arranged by size:
4545 1316 839
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2537 3668 4804 5705 5967 820
Size: 197 926 594 1131 1136 901 262 1553
Fragments arranged by size:
1553 1136 1131 926 901 594 262 197
SnaBI TAC'GTA
Cuts at: 493 4491 493
Size: 3998 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6700
SphI G_CATG'C
Cuts at: 1132 4058 1132
Size: 2926 3774
SspI AAT'ATT
Cuts at: 5 52 4624 5
Size: 47 4572 2081
Fragments arranged by size:
4572 2081 47
StuI AGG'CCT
Cuts at: 1517 2766 2796 1517
Size: 1249 30 5421
Fragments arranged by size:
5421 1249 30
StyI C'CwwG_G
Cuts at: 513 1065 2493 3336 3529 4139 513
Size: 552 1428 843 193 610 3074
Fragments arranged by size:
3074 1428 843 610 552 193
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2099 2140 2487 3548 4727 4744 4897
Size: 1255 519 41 347 1061 1179 17 153
Cuts at: 4897 5082 5527 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1179 1061 519 445 347 185
153 41 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2610 2724 2826
Size: 274 80 33 51 1443 633 114 102
Cuts at: 2826 3630 4946 6658 96
Size: 804 1316 1712 138
Fragments arranged by size:
1712 1443 1316 804 633 274 138 114
102 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2132 2164 6660 6692 2132
Size: 32 4496 32 2140
Fragments arranged by size:
4496 2140 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2279 1316
Size: 963 5737
XbaI T'CTAG_A
Cuts at: 4036 4036
Size: 6700
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6700
XmaI C'CCGG_G
Cuts at: 1870 3186 4025 1870
Size: 1316 839 4545
Fragments arranged by size:
4545 1316 839
XmnI GAAnn'nnTTC
Cuts at: 2663 3968 4829 2663
Size: 1305 861 4534
Fragments arranged by size:
4534 1305 861
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4508 276
Size: 53 83 186 936 2974 2468
Fragments arranged by size:
2974 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I
Bsu36I EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PspXI PsrI PvuII SanDI SexAI SfoI SrfI
SwaI XcmI