Restriction map for
pFN21AA0983
(Circular) (Six-base) MAPSORT of: pFN21AA0983.seq Check: 1975 from: 1 to: 6754
pFN21AA0983 6754 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2186 2802 3167 2186
Size: 616 365 5773
Fragments arranged by size:
5773 616 365
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4564 278
Size: 53 83 186 936 3028 2468
Fragments arranged by size:
3028 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4097 1937
Size: 2160 4594
Acc65I G'GTAC_C
Cuts at: 4075 4075
Size: 6754
AclI AA'CG_TT
Cuts at: 1563 4881 5254 1563
Size: 3318 373 3063
Fragments arranged by size:
3318 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2088 2240 3047 3080 3461 3973 4027 4817
Size: 456 152 807 33 381 512 54 790
Cuts at: 4817 6201 6469 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 807 790 512 456 381 268
152 54 33
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6557
AflIII A'CryG_T
Cuts at: 1203 5653 6630 1203
Size: 4450 977 1327
Fragments arranged by size:
4450 1327 977
AgeI A'CCGG_T
Cuts at: 5585 5585
Size: 6754
AhdI GACnn_n'nnGTC
Cuts at: 3187 5483 3187
Size: 2296 4458
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6754
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3038 1184
Size: 1854 4900
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2253 2285 2253
Size: 32 6722
AlwNI CAG_nnn'CTG
Cuts at: 2197 2401 3343 3889 4497 6069 2197
Size: 204 942 546 608 1572 2882
Fragments arranged by size:
2882 1572 942 608 546 204
ApaI G_GGCC'C
Cuts at: 3951 3951
Size: 6754
ApaLI G'TGCA_C
Cuts at: 4811 5967 4811
Size: 1156 5598
ApoI r'AATT_y
Cuts at: 1493 3956 4063 4289 4441 6550 1493
Size: 2463 107 226 152 2109 1697
Fragments arranged by size:
2463 2109 1697 226 152 107
AseI AT'TA_AT
Cuts at: 160 1032 5308 160
Size: 872 4276 1606
Fragments arranged by size:
4276 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6754
AvaI C'yCGr_G
Cuts at: 1870 1943 4079 1870
Size: 73 2136 4545
Fragments arranged by size:
4545 2136 73
BamHI G'GATC_C
Cuts at: 2268 4084 4451 2268
Size: 1816 367 4571
Fragments arranged by size:
4571 1816 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3033 4075 5530 618
Size: 325 217 627 1246 1042 1455 1842
Fragments arranged by size:
1842 1455 1246 1042 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2062 3951 4073 1391
Size: 671 1889 122 4072
Fragments arranged by size:
4072 1889 671 122
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3449 928
Size: 971 1550 4233
Fragments arranged by size:
4233 1550 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4945 4979 1903
Size: 34 3008 34 3678
Fragments arranged by size:
3678 3008 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3228 4646 5856 3228
Size: 1418 1210 4126
Fragments arranged by size:
4126 1418 1210
BclI T'GATC_A
Cuts at: 1548 3159 1548
Size: 1611 5143
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5365 136
Size: 107 122 71 631 605 3693 1525
Fragments arranged by size:
3693 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6749 6749
Size: 6754
BlpI GC'TnA_GC
Cuts at: 2361 3404 4171 2361
Size: 1043 767 4944
Fragments arranged by size:
4944 1043 767
Bme1580I G_kGCm'C
Cuts at: 1790 3683 3951 4815 5971 1790
Size: 1893 268 864 1156 2573
Fragments arranged by size:
2573 1893 1156 864 268
BmgBI CAC'GTC
Cuts at: 1318 2573 1318
Size: 1255 5499
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2590 2999 4485
Size: 416 53 83 399 24 1173 409 1486
Cuts at: 4485 5443 442
Size: 958 1753
Fragments arranged by size:
1753 1486 1173 958 416 409 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6754
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2959 2959
Size: 6754
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2378 2609 2675 2898 3935 5414 1239
Size: 1139 231 66 223 1037 1479 2579
Fragments arranged by size:
2579 1479 1139 1037 231 223 66
Bpu10I CC'TnA_GC
Cuts at: 2681 2681
Size: 6754
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2636 3329 4879 5744 6042 2636
Size: 693 1550 865 298 3348
Fragments arranged by size:
3348 1550 865 693 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2541 2974 3416 3911 5417 882
Size: 617 1042 433 442 495 1506 2219
Fragments arranged by size:
2219 1506 1042 617 495 442 433
BsaAI yAC'GTr
Cuts at: 493 1204 4545 493
Size: 711 3341 2702
Fragments arranged by size:
3341 2702 711
BsaBI GATnn'nnATC
Cuts at: 2379 6748 2379
Size: 4369 2385
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4561 4943 5578 275
Size: 53 83 186 936 3028 382 635 1451
Fragments arranged by size:
3028 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2728 5187 5585 5859 6006 6417 2728
Size: 2459 398 274 147 411 3065
Fragments arranged by size:
3065 2459 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2441 2471 2729 2759 3959
Size: 30 165 30 1038 30 258 30 1200
Cuts at: 3959 3989 1178
Size: 30 3943
Fragments arranged by size:
3943 1200 1038 258 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 2507 2969 2972 3715 1186
Size: 949 372 462 3 743 4225
Fragments arranged by size:
4225 949 743 462 372 3
BseYI C'CCAG_C
Cuts at: 2062 3343 3375 3638 3854 5957 6453 2062
Size: 1281 32 263 216 2103 496 2363
Fragments arranged by size:
2363 2103 1281 496 263 216 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2753 3195 3356 3473 3587 1163
Size: 573 1017 442 161 117 114 4330
Fragments arranged by size:
4330 1017 573 442 161 117 114
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4204 4965 5993 1540
Size: 275 186 2203 761 1028 2301
Fragments arranged by size:
2301 2203 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4815 4900 5971 4815
Size: 85 1071 5598
Fragments arranged by size:
5598 1071 85
BsmI GAATG_Cn'
Cuts at: 4265 4358 4265
Size: 93 6661
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2062 3683 3951 4073 4815 4900 5971
Size: 399 272 1621 268 122 742 85 1071
Cuts at: 5971 1391
Size: 2174
Fragments arranged by size:
2174 1621 1071 742 399 272 268 122
85
BspEI T'CCGG_A
Cuts at: 6417 6417
Size: 6754
BspHI T'CATG_A
Cuts at: 2289 3105 3120 4643 6373 2289
Size: 816 15 1523 1730 2670
Fragments arranged by size:
2670 1730 1523 816 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2186 2802 3167 4109 844
Size: 1342 616 365 942 3489
Fragments arranged by size:
3489 1342 942 616 365
BsrBI CCG'CTC
Cuts at: 1394 1823 4641 1394
Size: 429 2818 3507
Fragments arranged by size:
3507 2818 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5249 5423 65
Size: 1183 4001 174 1396
Fragments arranged by size:
4001 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5398 5585 1700
Size: 110 3588 187 2869
Fragments arranged by size:
3588 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6754
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6754
BssSI C'ACGA_G
Cuts at: 2354 4814 5826 2354
Size: 2460 1012 3282
Fragments arranged by size:
3282 2460 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6754
BstBI TT'CG_AA
Cuts at: 5558 5558
Size: 6754
BstEII G'GTnAC_C
Cuts at: 1184 3317 1184
Size: 2133 4621
BstXI CCAn_nnnn'nTGG
Cuts at: 2446 3418 2446
Size: 972 5782
BstYI r'GATC_y
Cuts at: 1972 2134 2208 2268 4084 4451 4836 4853 6305
Size: 162 74 60 1816 367 385 17 1452
Cuts at: 6305 6391 6403 6749 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1816 1452 385 367 346 162 86
74 60 17 12
Bsu36I CC'TnA_GG
Cuts at: 4032 4032
Size: 6754
BtgI C'CryG_G
Cuts at: 513 1065 2541 3150 3804 513
Size: 552 1476 609 654 3463
Fragments arranged by size:
3463 1476 654 609 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2067 4034 487
Size: 683 56 841 1967 3207
Fragments arranged by size:
3207 1967 841 683 56
BtsI GCAGTG_nn'
Cuts at: 2829 2887 4270 5064 5084 2829
Size: 58 1383 794 20 4499
Fragments arranged by size:
4499 1383 794 58 20
ClaI AT'CG_AT
Cuts at: 1554 2083 1554
Size: 529 6225
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6719
DraI TTT'AAA
Cuts at: 4018 4058 4018
Size: 40 6714
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5761 809
Size: 507 4445 1802
Fragments arranged by size:
4445 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4201 5090 6719
Size: 54 1425 50 275 18 2371 889 1629
Cuts at: 6719 8
Size: 43
Fragments arranged by size:
2371 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4201 1537
Size: 275 2389 4090
Fragments arranged by size:
4090 2389 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2216 2335 3023 3539 3992 4684 1353
Size: 863 119 688 516 453 692 3423
Fragments arranged by size:
3423 863 692 688 516 453 119
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5341 5715 5861 387
Size: 1064 138 3752 374 146 1280
Fragments arranged by size:
3752 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2088 2240 2378 2609 2675 2898 3047
Size: 393 456 152 138 231 66 223 149
Cuts at: 3047 3080 3461 3935 3973 4027 4817 5414 6201
Size: 33 381 474 38 54 790 597 787
Cuts at: 6201 6469 1239
Size: 268 1524
Fragments arranged by size:
1524 790 787 597 474 456 393 381
268 231 223 152 149 138 66 54
38 33
EcoNI CCTnn'n_nnAGG
Cuts at: 2426 2426
Size: 6754
EcoO109I rG'GnC_Cy
Cuts at: 2143 3947 3995 2143
Size: 1804 48 4902
Fragments arranged by size:
4902 1804 48
EcoRI G'AATT_C
Cuts at: 4063 4441 4063
Size: 378 6376
EcoRV GAT'ATC
Cuts at: 2521 2521
Size: 6754
FspI TGC'GCA
Cuts at: 5260 5260
Size: 6754
HaeII r_GCGC'y
Cuts at: 1678 2977 3411 3668 5901 1678
Size: 1299 434 257 2233 2531
Fragments arranged by size:
2531 2233 1299 434 257
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2441 2473 2615 2647 3173
Size: 32 135 32 869 32 142 32 526
Cuts at: 3173 3205 5395 5427 5469 5501 1373
Size: 32 2190 32 42 32 2626
Fragments arranged by size:
2626 2190 869 526 142 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3870 4098 4344 669
Size: 988 281 1932 228 246 3079
Fragments arranged by size:
3079 1932 988 281 246 228
HindIII A'AGCT_T
Cuts at: 748 2303 748
Size: 1555 5199
HpaI GTT'AAC
Cuts at: 4344 4344
Size: 6754
KpnI G_GTAC'C
Cuts at: 4079 4079
Size: 6754
MfeI C'AATT_G
Cuts at: 3576 4353 3576
Size: 777 5977
MluI A'CGCG_T
Cuts at: 6630 6630
Size: 6754
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2982 5868 6052 1305
Size: 1677 2886 184 2007
Fragments arranged by size:
2886 2007 1677 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4932
Fragments arranged by size:
4932 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4712 5071 5230 518
Size: 648 3546 359 159 2042
Fragments arranged by size:
3546 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2066 2596 3701 3865 3880 3886 4170 4847
Size: 460 530 1105 164 15 6 284 677
Cuts at: 4847 5995 6240 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1105 677 530 460 284 245
164 15 6
NcoI C'CATG_G
Cuts at: 513 1065 2541 3150 3804 513
Size: 552 1476 609 654 3463
Fragments arranged by size:
3463 1476 654 609 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6754
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6754
NotI GC'GGCC_GC
Cuts at: 4201 4201
Size: 6754
NspI r_CATG'y
Cuts at: 1132 1229 4112 5657 1132
Size: 97 2883 1545 2229
Fragments arranged by size:
2883 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6754
PciI A'CATG_T
Cuts at: 5653 5653
Size: 6754
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6754
PmeI GTTT'AAAC
Cuts at: 4058 4058
Size: 6754
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4823 4855 6336 6368 4823
Size: 32 1481 32 5209
Fragments arranged by size:
5209 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2143 3995 2143
Size: 1852 4902
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6754
PsiI TTA'TAA
Cuts at: 2502 4324 2502
Size: 1822 4932
PspOMI G'GGCC_C
Cuts at: 3947 3947
Size: 6754
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3704 3736 3704
Size: 32 6722
PstI C_TGCA'G
Cuts at: 830 2391 3696 3885 4106 830
Size: 1561 1305 189 221 3478
Fragments arranged by size:
3478 1561 1305 221 189
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6754
PvuII CAG'CTG
Cuts at: 2066 3865 3880 2066
Size: 1799 15 4940
Fragments arranged by size:
4940 1799 15
SalI G'TCGA_C
Cuts at: 1936 4096 1936
Size: 2160 4594
SanDI GG'GwC_CC
Cuts at: 2143 2143
Size: 6754
SapI GCTCTTCn'nnn_
Cuts at: 2216 3992 2216
Size: 1776 4978
SbfI CC_TGCA'GG
Cuts at: 4106 4106
Size: 6754
ScaI AGT'ACT
Cuts at: 5002 6714 5002
Size: 1712 5042
SfcI C'TryA_G
Cuts at: 826 1045 2113 2189 2387 2423 3692 3881 4102
Size: 219 1068 76 198 36 1269 189 221
Cuts at: 4102 5237 5918 6109 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1269 1135 1068 681 221 219 198
191 189 76 36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6754
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6754
SmaI CCC'GGG
Cuts at: 1872 4081 1872
Size: 2209 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2651 3308 4858 5759 6021 820
Size: 197 926 708 657 1550 901 262 1553
Fragments arranged by size:
1553 1550 926 901 708 657 262 197
SnaBI TAC'GTA
Cuts at: 493 4545 493
Size: 4052 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6754
SphI G_CATG'C
Cuts at: 1132 4112 1132
Size: 2980 3774
SspI AAT'ATT
Cuts at: 5 52 4678 5
Size: 47 4626 2081
Fragments arranged by size:
4626 2081 47
StuI AGG'CCT
Cuts at: 1517 2846 1517
Size: 1329 5425
StyI C'CwwG_G
Cuts at: 513 1065 2541 2895 3150 3543 3804 3848 3951
Size: 552 1476 354 255 393 261 44 103
Cuts at: 3951 4193 513
Size: 242 3074
Fragments arranged by size:
3074 1476 552 393 354 261 255 242
103 44
SwaI ATTT'AAAT
Cuts at: 4018 4018
Size: 6754
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3051 3322 3335 3559 3935 4781 4798
Size: 1255 1471 271 13 224 376 846 17
Cuts at: 4798 4951 5136 5581 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1471 1255 846 445 376 271 224
185 153 17 13
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2172 3304 5000
Size: 274 80 33 51 1443 195 1132 1696
Cuts at: 5000 6712 96
Size: 1712 138
Fragments arranged by size:
1712 1696 1443 1132 274 195 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3726 3758 3959 3991 6714 6746 3726
Size: 32 201 32 2723 32 3734
Fragments arranged by size:
3734 2723 201 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3200 1316
Size: 1884 4870
XbaI T'CTAG_A
Cuts at: 4090 4090
Size: 6754
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3619 3619
Size: 6754
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6754
XmaI C'CCGG_G
Cuts at: 1870 4079 1870
Size: 2209 4545
XmnI GAAnn'nnTTC
Cuts at: 4883 4883
Size: 6754
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4562 276
Size: 53 83 186 936 3028 2468
Fragments arranged by size:
3028 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI
BsmBI BstZ17I DraIII EcoICRI FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspXI RsrII SacI SacII SexAI SfiI SfoI SrfI