Restriction map for
pFN21AA0982
(Circular) (Six-base) MAPSORT of: pFN21AA0982.seq Check: 5624 from: 1 to: 6187
pFN21AA0982 6187 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2942 3997 278
Size: 53 83 186 936 1406 1055 2468
Fragments arranged by size:
2468 1406 1055 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3530 1937
Size: 1593 4594
Acc65I G'GTAC_C
Cuts at: 3508 3508
Size: 6187
AclI AA'CG_TT
Cuts at: 1563 2269 4314 4687 1563
Size: 706 2045 373 3063
Fragments arranged by size:
3063 2045 706 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2301 2390 4250 5634 5902 1632
Size: 669 89 1860 1384 268 1917
Fragments arranged by size:
1917 1860 1384 669 268 89
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5990
AflIII A'CryG_T
Cuts at: 1203 2127 5086 6063 1203
Size: 924 2959 977 1327
Fragments arranged by size:
2959 1327 977 924
AgeI A'CCGG_T
Cuts at: 5018 5018
Size: 6187
AhdI GACnn_n'nnGTC
Cuts at: 4916 4916
Size: 6187
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6187
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6187
AlwNI CAG_nnn'CTG
Cuts at: 2368 3930 5502 2368
Size: 1562 1572 3053
Fragments arranged by size:
3053 1572 1562
ApaLI G'TGCA_C
Cuts at: 4244 5400 4244
Size: 1156 5031
ApoI r'AATT_y
Cuts at: 1493 2207 2611 2621 3496 3722 3874 5983 1493
Size: 714 404 10 875 226 152 2109 1697
Fragments arranged by size:
2109 1697 875 714 404 226 152 10
AseI AT'TA_AT
Cuts at: 160 1032 4741 160
Size: 872 3709 1606
Fragments arranged by size:
3709 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6187
AvaI C'yCGr_G
Cuts at: 1870 1943 2519 3512 1870
Size: 73 576 993 4545
Fragments arranged by size:
4545 993 576 73
BamHI G'GATC_C
Cuts at: 3517 3884 3517
Size: 367 5820
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3508 4963 618
Size: 325 217 627 1721 1455 1842
Fragments arranged by size:
1842 1721 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2606 2775 2949 3506 1391
Size: 1215 169 174 557 4072
Fragments arranged by size:
4072 1215 557 174 169
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2378 3402 928
Size: 971 479 1024 3713
Fragments arranged by size:
3713 1024 971 479
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2010 2021 2044 4378 4412 1903
Size: 34 50 23 11 23 2334 34 3678
Fragments arranged by size:
3678 2334 50 34 34 23 23 11
BciVI GTATCCnnnnn_n'
Cuts at: 4079 5289 4079
Size: 1210 4977
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6187
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2601 2847 3357
Size: 107 122 71 631 605 929 246 510
Cuts at: 3357 4798 136
Size: 1441 1525
Fragments arranged by size:
1525 1441 929 631 605 510 246 122
107 71
BglII A'GATC_T
Cuts at: 6182 6182
Size: 6187
BlpI GC'TnA_GC
Cuts at: 3604 3604
Size: 6187
Bme1580I G_kGCm'C
Cuts at: 1790 2970 4248 5404 1790
Size: 1180 1278 1156 2573
Fragments arranged by size:
2573 1278 1180 1156
BmgBI CAC'GTC
Cuts at: 1318 2932 1318
Size: 1614 4573
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3918 4876 442
Size: 416 53 83 399 24 2501 958 1753
Fragments arranged by size:
2501 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2392 2679 2701 4847 1239
Size: 1153 287 22 2146 2579
Fragments arranged by size:
2579 2146 1153 287 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2819 4312 5177 5475 2819
Size: 1493 865 298 3531
Fragments arranged by size:
3531 1493 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4850 882
Size: 617 3351 2219
Fragments arranged by size:
3351 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2243 2599 3978 493
Size: 711 1039 356 1379 2702
Fragments arranged by size:
2702 1379 1039 711 356
BsaBI GATnn'nnATC
Cuts at: 6181 6181
Size: 6187
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2939 3994 4376 5011
Size: 53 83 186 936 1406 1055 382 635
Cuts at: 5011 275
Size: 1451
Fragments arranged by size:
1451 1406 1055 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2910 4620 5018 5292 5439 5850 2910
Size: 1710 398 274 147 411 3247
Fragments arranged by size:
3247 1710 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3129 3159 1178
Size: 30 165 30 1726 30 4206
Fragments arranged by size:
4206 1726 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3016 1186
Size: 1830 4357
BseYI C'CCAG_C
Cuts at: 2709 3006 5390 5886 2709
Size: 297 2384 496 3010
Fragments arranged by size:
3010 2384 496 297
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2714 2977 1163
Size: 573 978 263 4373
Fragments arranged by size:
4373 978 573 263
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3637 4398 5426 1540
Size: 275 186 1636 761 1028 2301
Fragments arranged by size:
2301 1636 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2520 2759 2949 4248 4333 5404 2520
Size: 239 190 1299 85 1071 3303
Fragments arranged by size:
3303 1299 1071 239 190 85
BsmI GAATG_Cn'
Cuts at: 3698 3791 3698
Size: 93 6094
BsmBI CGTCTCn'nnnn_
Cuts at: 2930 2930
Size: 6187
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2520 2606 2759 2775 2949 2970 3506
Size: 399 730 86 153 16 174 21 536
Cuts at: 3506 4248 4333 5404 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 730 536 399 174 153
86 85 21 16
BspEI T'CCGG_A
Cuts at: 5850 5850
Size: 6187
BspHI T'CATG_A
Cuts at: 4076 5806 4076
Size: 1730 4457
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3542 844
Size: 2698 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4074 1394
Size: 429 2251 3507
Fragments arranged by size:
3507 2251 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3207 3470 4682 4856 65
Size: 1183 1959 263 1212 174 1396
Fragments arranged by size:
1959 1396 1212 1183 263 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3435 4831 5018 1700
Size: 110 1625 1396 187 2869
Fragments arranged by size:
2869 1625 1396 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6187
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6187
BssSI C'ACGA_G
Cuts at: 2447 3014 4247 5259 2447
Size: 567 1233 1012 3375
Fragments arranged by size:
3375 1233 1012 567
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6187
BstBI TT'CG_AA
Cuts at: 4991 4991
Size: 6187
BstEII G'GTnAC_C
Cuts at: 1184 3019 1184
Size: 1835 4352
BstYI r'GATC_y
Cuts at: 1972 3200 3517 3884 4269 4286 5738 5824 5836
Size: 1228 317 367 385 17 1452 86 12
Cuts at: 5836 6182 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1228 385 367 346 317 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3132 513
Size: 552 2067 3568
Fragments arranged by size:
3568 2067 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2842 3174 487
Size: 683 56 1616 332 3500
Fragments arranged by size:
3500 1616 683 332 56
BtsI GCAGTG_nn'
Cuts at: 2649 2986 3703 4497 4517 2649
Size: 337 717 794 20 4319
Fragments arranged by size:
4319 794 717 337 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6187
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6152
DraI TTT'AAA
Cuts at: 3491 3491
Size: 6187
DraIII CAC_nnn'GTG
Cuts at: 3082 3406 3082
Size: 324 5863
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5194 809
Size: 507 3878 1802
Fragments arranged by size:
3878 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2464 2496 3259
Size: 54 1425 50 275 18 634 32 763
Cuts at: 3259 3634 4523 6152 8
Size: 375 889 1629 43
Fragments arranged by size:
1629 1425 889 763 634 375 275 54
50 43 32 18
EagI C'GGCC_G
Cuts at: 1537 1812 3634 1537
Size: 275 1822 4090
Fragments arranged by size:
4090 1822 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4117 1353
Size: 2764 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3035 4774 5148 5294 387
Size: 1064 138 1446 1739 374 146 1280
Fragments arranged by size:
1739 1446 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2947 2947
Size: 6187
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2301 2390 2392 2679 2701 4250 4847
Size: 393 669 89 2 287 22 1549 597
Cuts at: 4847 5634 5902 1239
Size: 787 268 1524
Fragments arranged by size:
1549 1524 787 669 597 393 287 268
89 22 2
EcoO109I rG'GnC_Cy
Cuts at: 3064 3064
Size: 6187
EcoRI G'AATT_C
Cuts at: 3496 3874 3496
Size: 378 5809
FspI TGC'GCA
Cuts at: 4693 4693
Size: 6187
HaeII r_GCGC'y
Cuts at: 1678 5334 1678
Size: 3656 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4828 4860 4902 4934 1373
Size: 32 135 32 3256 32 42 32 2626
Fragments arranged by size:
3256 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2982 3531 3777 669
Size: 988 281 1044 549 246 3079
Fragments arranged by size:
3079 1044 988 549 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6187
HpaI GTT'AAC
Cuts at: 3777 3777
Size: 6187
KpnI G_GTAC'C
Cuts at: 3512 3512
Size: 6187
MfeI C'AATT_G
Cuts at: 3786 3786
Size: 6187
MluI A'CGCG_T
Cuts at: 6063 6063
Size: 6187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2889 3431 5301 5485 1305
Size: 1584 542 1870 184 2007
Fragments arranged by size:
2007 1870 1584 542 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2498 10
Size: 54 1425 343 666 3699
Fragments arranged by size:
3699 1425 666 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4145 4504 4663 518
Size: 648 2979 359 159 2042
Fragments arranged by size:
2979 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2698 3010 3603 4280 5428 5673 1606
Size: 1092 312 593 677 1148 245 2120
Fragments arranged by size:
2120 1148 1092 677 593 312 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5635
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6187
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6187
NotI GC'GGCC_GC
Cuts at: 3634 3634
Size: 6187
NspI r_CATG'y
Cuts at: 1132 1229 2131 3545 5090 1132
Size: 97 902 1414 1545 2229
Fragments arranged by size:
2229 1545 1414 902 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6187
PciI A'CATG_T
Cuts at: 2127 5086 2127
Size: 2959 3228
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2851 3450 1108
Size: 1743 599 3845
Fragments arranged by size:
3845 1743 599
PmeI GTTT'AAAC
Cuts at: 3491 3491
Size: 6187
PmlI CAC'GTG
Cuts at: 2599 2599
Size: 6187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4256 4288 5769 5801 4256
Size: 32 1481 32 4642
Fragments arranged by size:
4642 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3064 3064
Size: 6187
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6187
PsiI TTA'TAA
Cuts at: 3757 3757
Size: 6187
PstI C_TGCA'G
Cuts at: 830 3539 830
Size: 2709 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6187
PvuII CAG'CTG
Cuts at: 2698 2698
Size: 6187
SacI G_AGCT'C
Cuts at: 2949 2949
Size: 6187
SalI G'TCGA_C
Cuts at: 1936 3529 1936
Size: 1593 4594
SanDI GG'GwC_CC
Cuts at: 3064 3064
Size: 6187
SbfI CC_TGCA'GG
Cuts at: 3539 3539
Size: 6187
ScaI AGT'ACT
Cuts at: 4435 6147 4435
Size: 1712 4475
SfcI C'TryA_G
Cuts at: 826 1045 3535 4670 5351 5542 826
Size: 219 2490 1135 681 191 1471
Fragments arranged by size:
2490 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6187
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6187
SmaI CCC'GGG
Cuts at: 1872 3514 1872
Size: 1642 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2834 4291 5192 5454 820
Size: 197 926 891 1457 901 262 1553
Fragments arranged by size:
1553 1457 926 901 891 262 197
SnaBI TAC'GTA
Cuts at: 493 2243 3978 493
Size: 1750 1735 2702
Fragments arranged by size:
2702 1750 1735
SpeI A'CTAG_T
Cuts at: 152 2726 152
Size: 2574 3613
SphI G_CATG'C
Cuts at: 1132 3545 1132
Size: 2413 3774
SspI AAT'ATT
Cuts at: 5 52 4111 5
Size: 47 4059 2081
Fragments arranged by size:
4059 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6187
StyI C'CwwG_G
Cuts at: 513 1065 3626 513
Size: 552 2561 3074
Fragments arranged by size:
3074 2561 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3020 4214 4231 4384 4569 5014 325
Size: 1255 1440 1194 17 153 185 445 1498
Fragments arranged by size:
1498 1440 1255 1194 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2171 2457 4433
Size: 274 80 33 51 1443 194 286 1976
Cuts at: 4433 6145 96
Size: 1712 138
Fragments arranged by size:
1976 1712 1443 286 274 194 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3129 3161 6147 6179 3129
Size: 32 2986 32 3137
Fragments arranged by size:
3137 2986 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6187
XbaI T'CTAG_A
Cuts at: 3523 3523
Size: 6187
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6187
XmaI C'CCGG_G
Cuts at: 1870 3512 1870
Size: 1642 4545
XmnI GAAnn'nnTTC
Cuts at: 3089 4316 3089
Size: 1227 4960
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2940 3995 276
Size: 53 83 186 936 1406 1055 2468
Fragments arranged by size:
2468 1406 1055 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI
RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI
XcmI