Restriction map for
pFN21AA0973
(Circular) (Six-base) MAPSORT of: pFN21AA0973.seq Check: 3519 from: 1 to: 9415
pFN21AA0973 9415 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2916 2916
Size: 9415
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6162 7225 278
Size: 53 83 186 936 4626 1063 2468
Fragments arranged by size:
4626 2468 1063 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2547 2733 3168 4962 6758 1937
Size: 610 186 435 1794 1796 4594
Fragments arranged by size:
4594 1796 1794 610 435 186
Acc65I G'GTAC_C
Cuts at: 6304 6736 6304
Size: 432 8983
AclI AA'CG_TT
Cuts at: 1563 7542 7915 1563
Size: 5979 373 3063
Fragments arranged by size:
5979 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3413 4120 4408 6672 7478 8862 9130 1632
Size: 1781 707 288 2264 806 1384 268 1917
Fragments arranged by size:
2264 1917 1781 1384 806 707 288 268
AfeI AGC'GCT
Cuts at: 3200 3408 4919 6440 3200
Size: 208 1511 1521 6175
Fragments arranged by size:
6175 1521 1511 208
AflII C'TTAA_G
Cuts at: 820 1017 5126 820
Size: 197 4109 5109
Fragments arranged by size:
5109 4109 197
AflIII A'CryG_T
Cuts at: 1203 8314 9291 1203
Size: 7111 977 1327
Fragments arranged by size:
7111 1327 977
AgeI A'CCGG_T
Cuts at: 8246 8246
Size: 9415
AhdI GACnn_n'nnGTC
Cuts at: 8144 8144
Size: 9415
AleI CACnn'nnGTG
Cuts at: 1166 5069 1166
Size: 3903 5512
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3219 5940 1184
Size: 2035 2721 4659
Fragments arranged by size:
4659 2721 2035
AlwNI CAG_nnn'CTG
Cuts at: 4751 6361 7158 8730 4751
Size: 1610 797 1572 5436
Fragments arranged by size:
5436 1610 1572 797
ApaI G_GGCC'C
Cuts at: 4238 4516 4238
Size: 278 9137
ApaLI G'TGCA_C
Cuts at: 3485 6109 6558 7472 8628 3485
Size: 2624 449 914 1156 4272
Fragments arranged by size:
4272 2624 1156 914 449
ApoI r'AATT_y
Cuts at: 1493 2882 6724 6950 7102 9211 1493
Size: 1389 3842 226 152 2109 1697
Fragments arranged by size:
3842 2109 1697 1389 226 152
AscI GG'CGCG_CC
Cuts at: 5967 5967
Size: 9415
AseI AT'TA_AT
Cuts at: 160 1032 7969 160
Size: 872 6937 1606
Fragments arranged by size:
6937 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9415
AvaI C'yCGr_G
Cuts at: 1870 1943 2495 2688 2891 3621 3671 3824 4238
Size: 73 552 193 203 730 50 153 414
Cuts at: 4238 4921 5377 5485 5864 6044 6091 6332 6416
Size: 683 456 108 379 180 47 241 84
Cuts at: 6416 6426 6607 6706 6740 1870
Size: 10 181 99 34 4545
Fragments arranged by size:
4545 730 683 552 456 414 379 241
203 193 181 180 153 108 99 84
73 50 47 34 10
AvrII C'CTAG_G
Cuts at: 2173 5452 2173
Size: 3279 6136
BamHI G'GATC_C
Cuts at: 4496 6745 7112 4496
Size: 2249 367 6799
Fragments arranged by size:
6799 2249 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2303 3152 3180 3336 4382
Size: 325 217 627 516 849 28 156 1046
Cuts at: 4382 4541 4668 5456 6050 6098 6304 6349 6403
Size: 159 127 788 594 48 206 45 54
Cuts at: 6403 6611 6642 6736 8191 618
Size: 208 31 94 1455 1842
Fragments arranged by size:
1842 1455 1046 849 788 627 594 516
325 217 208 206 159 156 127 94
54 48 45 31 28
BanII G_rGCy'C
Cuts at: 1391 2394 3573 4098 4182 4238 4313 4416 4516
Size: 1003 1179 525 84 56 75 103 100
Cuts at: 4516 4896 5384 5769 6245 6525 6608 6734 1391
Size: 380 488 385 476 280 83 126 4072
Fragments arranged by size:
4072 1179 1003 525 488 476 385 380
280 126 103 100 84 83 75 56
BbeI G_GCGC'C
Cuts at: 3340 4386 5460 6102 6407 3340
Size: 1046 1074 642 305 6348
Fragments arranged by size:
6348 1074 1046 642 305
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2960 4301 928
Size: 971 1061 1341 6042
Fragments arranged by size:
6042 1341 1061 971
BbvCI CC'TCA_GC
Cuts at: 2489 3971 4744 6354 2489
Size: 1482 773 1610 5550
Fragments arranged by size:
5550 1610 1482 773
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5299 5333 5510 5544 7606 7640 1903
Size: 34 3362 34 177 34 2062 34 3678
Fragments arranged by size:
3678 3362 2062 177 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7307 8517 7307
Size: 1210 8205
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9415
BfrBI ATG'CAT
Cuts at: 4622 4622
Size: 9415
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2988 4474 5036
Size: 107 122 71 631 605 1316 1486 562
Cuts at: 5036 5997 6233 6328 8026 136
Size: 961 236 95 1698 1525
Fragments arranged by size:
1698 1525 1486 1316 961 631 605 562
236 122 107 95 71
BglII A'GATC_T
Cuts at: 9410 9410
Size: 9415
BlpI GC'TnA_GC
Cuts at: 2654 6239 6476 6832 2654
Size: 3585 237 356 5237
Fragments arranged by size:
5237 3585 356 237
Bme1580I G_kGCm'C
Cuts at: 1790 3489 3762 4238 4516 6113 6296 6417 6562
Size: 1699 273 476 278 1597 183 121 145
Cuts at: 6562 6614 6647 6663 7476 8632 1790
Size: 52 33 16 813 1156 2573
Fragments arranged by size:
2573 1699 1597 1156 813 476 278 273
183 145 121 52 33 16
BmgBI CAC'GTC
Cuts at: 1318 2545 3016 1318
Size: 1227 471 7717
Fragments arranged by size:
7717 1227 471
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2310 4184 4728
Size: 416 53 83 399 24 893 1874 544
Cuts at: 4728 5945 6575 7146 8104 442
Size: 1217 630 571 958 1753
Fragments arranged by size:
1874 1753 1217 958 893 630 571 544
416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3455 1056
Size: 2399 7016
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4728 6070 6501 4728
Size: 1342 431 7642
Fragments arranged by size:
7642 1342 431
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2780 2891 2933 3047 3479 4321 4376 4430
Size: 1541 111 42 114 432 842 55 54
Cuts at: 4430 4531 6040 6170 8075 1239
Size: 101 1509 130 1905 2579
Fragments arranged by size:
2579 1905 1541 1509 842 432 130 114
111 101 55 54 42
Bpu10I CC'TnA_GC
Cuts at: 2489 3060 3971 4744 6339 6354 2489
Size: 571 911 773 1595 15 5550
Fragments arranged by size:
5550 1595 911 773 571 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3482 4515 5771 6606 7540 8405 8703 3482
Size: 1033 1256 835 934 865 298 4194
Fragments arranged by size:
4194 1256 1033 934 865 835 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2140 2277 3942 8078 882
Size: 617 641 137 1665 4136 2219
Fragments arranged by size:
4136 2219 1665 641 617 137
BsaAI yAC'GTr
Cuts at: 493 1204 4057 5071 7206 493
Size: 711 2853 1014 2135 2702
Fragments arranged by size:
2853 2702 2135 1014 711
BsaBI GATnn'nnATC
Cuts at: 9409 9409
Size: 9415
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2263 2778 3337 3618
Size: 53 83 186 936 730 515 559 281
Cuts at: 3618 4383 5457 5516 5651 5945 6099 6159 6404
Size: 765 1074 59 135 294 154 60 245
Cuts at: 6404 7222 7604 8239 275
Size: 818 382 635 1451
Fragments arranged by size:
1451 1074 936 818 765 730 635 559
515 382 294 281 245 186 154 135
83 60 59 53
BsaWI w'CCGG_w
Cuts at: 4315 5289 7848 8246 8520 8667 9078 4315
Size: 974 2559 398 274 147 411 4652
Fragments arranged by size:
4652 2559 974 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2387 2417 4046 4076 4151
Size: 30 165 30 984 30 1629 30 75
Cuts at: 4151 4181 1178
Size: 30 6412
Fragments arranged by size:
6412 1629 984 165 75 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2233 2332 2500 2600 2909 3797 3851 4103
Size: 1047 99 168 100 309 888 54 252
Cuts at: 4103 4106 4256 4538 4846 5774 6476 1186
Size: 3 150 282 308 928 702 4125
Fragments arranged by size:
4125 1047 928 888 702 309 308 282
252 168 150 100 99 54 3
BseYI C'CCAG_C
Cuts at: 2461 2986 4270 4415 4478 4862 5026 5051 5479
Size: 525 1284 145 63 384 164 25 428
Cuts at: 5479 5534 5812 6441 6544 6563 6664 8618 9114
Size: 55 278 629 103 19 101 1954 496
Cuts at: 9114 2461
Size: 2762
Fragments arranged by size:
2762 1954 1284 629 525 496 428 384
278 164 145 103 101 63 55 25
19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5528 5705 5816 6151 6206 6269 6574
Size: 573 3792 177 111 335 55 63 305
Cuts at: 6574 6591 1163
Size: 17 3987
Fragments arranged by size:
3987 3792 573 335 305 177 111 63
55 17
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2922 5601 5805 6865 7626 8654
Size: 275 186 921 2679 204 1060 761 1028
Cuts at: 8654 1540
Size: 2301
Fragments arranged by size:
2679 2301 1060 1028 921 761 275 204
186
BsiHKAI G_wGCw'C
Cuts at: 2394 2423 2655 3489 4229 5492 6113 6562 7476
Size: 29 232 834 740 1263 621 449 914
Cuts at: 7476 7561 8632 2394
Size: 85 1071 3177
Fragments arranged by size:
3177 1263 1071 914 834 740 621 449
232 85 29
BsmI GAATG_Cn'
Cuts at: 6926 7019 6926
Size: 93 9322
BsmBI CGTCTCn'nnnn_
Cuts at: 2332 3437 5936 2332
Size: 1105 2499 5811
Fragments arranged by size:
5811 2499 1105
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2394 2423 2655 3489 3573 3762 4098
Size: 399 604 29 232 834 84 189 336
Cuts at: 4098 4182 4229 4238 4313 4416 4516 4896 5384
Size: 84 47 9 75 103 100 380 488
Cuts at: 5384 5492 5769 6113 6245 6296 6417 6525 6562
Size: 108 277 344 132 51 121 108 37
Cuts at: 6562 6608 6614 6647 6663 6734 7476 7561 8632
Size: 46 6 33 16 71 742 85 1071
Cuts at: 8632 1391
Size: 2174
Fragments arranged by size:
2174 1071 834 742 604 488 399 380
344 336 277 232 189 132 121 108
108 103 100 85 84 84 75 71
51 47 46 37 33 29 16 9
6
BspEI T'CCGG_A
Cuts at: 4315 5289 9078 4315
Size: 974 3789 4652
Fragments arranged by size:
4652 3789 974
BspHI T'CATG_A
Cuts at: 3573 4764 7304 9034 3573
Size: 1191 2540 1730 3954
Fragments arranged by size:
3954 2540 1730 1191
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2916 5322 5968 6770 844
Size: 2072 2406 646 802 3489
Fragments arranged by size:
3489 2406 2072 802 646
BsrBI CCG'CTC
Cuts at: 1394 1823 2478 4038 4899 5331 5352 5430 5580
Size: 429 655 1560 861 432 21 78 150
Cuts at: 5580 5782 6154 6480 7302 1394
Size: 202 372 326 822 3507
Fragments arranged by size:
3507 1560 861 822 655 432 429 372
326 202 150 78 21
BsrDI GCAATG_nn'
Cuts at: 65 1248 7910 8084 65
Size: 1183 6662 174 1396
Fragments arranged by size:
6662 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2265 4253 5401 5600 6267 8059 8246
Size: 110 455 1988 1148 199 667 1792 187
Cuts at: 8246 1700
Size: 2869
Fragments arranged by size:
2869 1988 1792 1148 667 455 199 187
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9415
BssHII G'CGCG_C
Cuts at: 1925 5405 5844 5967 1925
Size: 3480 439 123 5373
Fragments arranged by size:
5373 3480 439 123
BssSI C'ACGA_G
Cuts at: 4175 6257 7475 8487 4175
Size: 2082 1218 1012 5103
Fragments arranged by size:
5103 2082 1218 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3318 5398 794
Size: 2524 2080 4811
Fragments arranged by size:
4811 2524 2080
BstBI TT'CG_AA
Cuts at: 5166 8219 5166
Size: 3053 6362
BstEII G'GTnAC_C
Cuts at: 1184 2168 4589 1184
Size: 984 2421 6010
Fragments arranged by size:
6010 2421 984
BstXI CCAn_nnnn'nTGG
Cuts at: 3711 3892 4354 4648 5075 6223 3711
Size: 181 462 294 427 1148 6903
Fragments arranged by size:
6903 1148 462 427 294 181
BstYI r'GATC_y
Cuts at: 1972 3249 4496 6745 7112 7497 7514 8966 9052
Size: 1277 1247 2249 367 385 17 1452 86
Cuts at: 9052 9064 9410 1972
Size: 12 346 1977
Fragments arranged by size:
2249 1977 1452 1277 1247 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5106 5106
Size: 9415
BtgI C'CryG_G
Cuts at: 513 1065 2379 2727 3525 4098 4491 5899 513
Size: 552 1314 348 798 573 393 1408 4029
Fragments arranged by size:
4029 1408 1314 798 573 552 393 348
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3649 5552 5602 5712 487
Size: 683 56 2423 1903 50 110 4190
Fragments arranged by size:
4190 2423 1903 683 110 56 50
BtsI GCAGTG_nn'
Cuts at: 2571 4749 4782 4943 5154 5663 6562 6574 6579
Size: 2178 33 161 211 509 899 12 5
Cuts at: 6579 6931 7725 7745 2571
Size: 352 794 20 4241
Fragments arranged by size:
4241 2178 899 794 509 352 211 161
33 20 12 5
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9415
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9380
DraI TTT'AAA
Cuts at: 6719 6719
Size: 9415
DraIII CAC_nnn'GTG
Cuts at: 2950 4057 4949 5680 2950
Size: 1107 892 731 6685
Fragments arranged by size:
6685 1107 892 731
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4979 8422 809
Size: 507 3663 3443 1802
Fragments arranged by size:
3663 3443 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2449 2640 2919
Size: 54 1425 50 275 18 619 191 279
Cuts at: 2919 4251 5598 5712 5802 6139 6213 6225 6862
Size: 1332 1347 114 90 337 74 12 637
Cuts at: 6862 7751 9380 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1347 1332 889 637 619 337
279 275 191 114 90 74 54 50
43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2919 5598 5802 6862 1537
Size: 275 1107 2679 204 1060 4090
Fragments arranged by size:
4090 2679 1107 1060 275 204
EarI CTCTTCn'nnn_
Cuts at: 1353 4123 4697 5291 7345 1353
Size: 2770 574 594 2054 3423
Fragments arranged by size:
3423 2770 2054 594 574
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2063 2270 2969 4097 5236 5434
Size: 1064 138 474 207 699 1128 1139 198
Cuts at: 5434 5978 8002 8376 8522 387
Size: 544 2024 374 146 1280
Fragments arranged by size:
2024 1280 1139 1128 1064 699 544 474
374 207 198 146 138
EcoICRI GAG'CTC
Cuts at: 2392 2392
Size: 9415
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2780 2891 2933 3047 3413 3479 4120
Size: 393 1148 111 42 114 366 66 641
Cuts at: 4120 4321 4376 4408 4430 4531 6040 6170 6672
Size: 201 55 32 22 101 1509 130 502
Cuts at: 6672 7478 8075 8862 9130 1239
Size: 806 597 787 268 1524
Fragments arranged by size:
1524 1509 1148 806 787 641 597 502
393 366 268 201 130 114 111 101
66 55 42 32 22
EcoNI CCTnn'n_nnAGG
Cuts at: 2248 2912 2248
Size: 664 8751
EcoO109I rG'GnC_Cy
Cuts at: 3651 3838 4234 4235 4513 5056 5959 6421 3651
Size: 187 396 1 278 543 903 462 6645
Fragments arranged by size:
6645 903 543 462 396 278 187 1
EcoRI G'AATT_C
Cuts at: 2882 6724 7102 2882
Size: 3842 378 5195
Fragments arranged by size:
5195 3842 378
EcoRV GAT'ATC
Cuts at: 3277 3277
Size: 9415
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3247 4954 3247
Size: 1707 7708
FspI TGC'GCA
Cuts at: 2577 4463 5852 7921 2577
Size: 1886 1389 2069 4071
Fragments arranged by size:
4071 2069 1886 1389
HaeII r_GCGC'y
Cuts at: 1678 2356 2503 3165 3202 3340 3410 3918 4386
Size: 678 147 662 37 138 70 508 468
Cuts at: 4386 4406 4431 4554 4921 5460 5915 6102 6239
Size: 20 25 123 367 539 455 187 137
Cuts at: 6239 6407 6442 8562 1678
Size: 168 35 2120 2531
Fragments arranged by size:
2531 2120 678 662 539 508 468 455
367 187 168 147 138 137 123 70
37 35 25 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2524 2556 3059 3091 3786
Size: 32 135 32 952 32 503 32 695
Cuts at: 3786 3818 3874 3906 4022 4054 4151 4183 6071
Size: 32 56 32 116 32 97 32 1888
Cuts at: 6071 6103 8056 8088 8130 8162 1373
Size: 32 1953 32 42 32 2626
Fragments arranged by size:
2626 1953 1888 952 695 503 135 116
97 56 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 6759 7005 669
Size: 988 281 4821 246 3079
Fragments arranged by size:
4821 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9415
HpaI GTT'AAC
Cuts at: 7005 7005
Size: 9415
KasI G'GCGC_C
Cuts at: 3336 4382 5456 6098 6403 3336
Size: 1046 1074 642 305 6348
Fragments arranged by size:
6348 1074 1046 642 305
KpnI G_GTAC'C
Cuts at: 6308 6740 6308
Size: 432 8983
MfeI C'AATT_G
Cuts at: 7014 7014
Size: 9415
MluI A'CGCG_T
Cuts at: 9291 9291
Size: 9415
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3075 3987 4509 4860 5958 6429 8529 8713
Size: 1770 912 522 351 1098 471 2100 184
Cuts at: 8713 1305
Size: 2007
Fragments arranged by size:
2100 2007 1770 1098 912 522 471 351
184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 6215 6227 10
Size: 54 1425 343 4383 12 3198
Fragments arranged by size:
4383 3198 1425 343 54 12
MslI CAynn'nnrTG
Cuts at: 518 1166 3353 3474 3524 5069 5073 7373 7732
Size: 648 2187 121 50 1545 4 2300 359
Cuts at: 7732 7891 518
Size: 159 2042
Fragments arranged by size:
2300 2187 2042 1545 648 359 159 121
50 4
MspA1I CmG'CkG
Cuts at: 1606 2161 2365 2824 2986 4165 4199 4493 4883
Size: 555 204 459 162 1179 34 294 390
Cuts at: 4883 5623 6831 7508 8656 8901 1606
Size: 740 1208 677 1148 245 2120
Fragments arranged by size:
2120 1208 1179 1148 740 677 555 459
390 294 245 204 162 34
NaeI GCC'GGC
Cuts at: 4255 5403 5602 6269 4255
Size: 1148 199 667 7401
Fragments arranged by size:
7401 1148 667 199
NarI GG'CG_CC
Cuts at: 3337 4383 5457 6099 6404 3337
Size: 1046 1074 642 305 6348
Fragments arranged by size:
6348 1074 1046 642 305
NcoI C'CATG_G
Cuts at: 513 1065 3525 5899 513
Size: 552 2460 2374 4029
Fragments arranged by size:
4029 2460 2374 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9415
NgoMIV G'CCGG_C
Cuts at: 4253 5401 5600 6267 4253
Size: 1148 199 667 7401
Fragments arranged by size:
7401 1148 667 199
NheI G'CTAG_C
Cuts at: 1052 3451 1052
Size: 2399 7016
NotI GC'GGCC_GC
Cuts at: 6862 6862
Size: 9415
NsiI A_TGCA'T
Cuts at: 4624 4624
Size: 9415
NspI r_CATG'y
Cuts at: 1132 1229 3316 3433 5012 6773 8318 1132
Size: 97 2087 117 1579 1761 1545 2229
Fragments arranged by size:
2229 2087 1761 1579 1545 117 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9415
PciI A'CATG_T
Cuts at: 8314 8314
Size: 9415
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2911 5680 1108
Size: 1803 2769 4843
Fragments arranged by size:
4843 2769 1803
PfoI T'CCnGG_A
Cuts at: 3630 4353 3630
Size: 723 8692
PmeI GTTT'AAAC
Cuts at: 6719 6719
Size: 9415
PmlI CAC'GTG
Cuts at: 4057 5071 4057
Size: 1014 8401
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7484 7516 8997 9029 7484
Size: 32 1481 32 7870
Fragments arranged by size:
7870 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3651 5056 3651
Size: 1405 8010
PshAI GACnn'nnGTC
Cuts at: 1613 6007 6581 1613
Size: 4394 574 4447
Fragments arranged by size:
4447 4394 574
PsiI TTA'TAA
Cuts at: 6985 6985
Size: 9415
PspOMI G'GGCC_C
Cuts at: 4234 4512 4234
Size: 278 9137
PspXI vC'TCGA_Gb
Cuts at: 5485 5864 5485
Size: 379 9036
PstI C_TGCA'G
Cuts at: 830 3882 4204 4777 6767 830
Size: 3052 322 573 1990 3478
Fragments arranged by size:
3478 3052 1990 573 322
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9415
PvuII CAG'CTG
Cuts at: 2365 2824 2986 4165 4199 4883 2365
Size: 459 162 1179 34 684 6897
Fragments arranged by size:
6897 1179 684 459 162 34
SacI G_AGCT'C
Cuts at: 2394 2394
Size: 9415
SacII CC_GC'GG
Cuts at: 4494 4494
Size: 9415
SalI G'TCGA_C
Cuts at: 1936 6757 1936
Size: 4821 4594
SanDI GG'GwC_CC
Cuts at: 3651 3651
Size: 9415
SbfI CC_TGCA'GG
Cuts at: 6767 6767
Size: 9415
ScaI AGT'ACT
Cuts at: 7663 9375 7663
Size: 1712 7703
SexAI A'CCwGG_T
Cuts at: 3171 3171
Size: 9415
SfcI C'TryA_G
Cuts at: 826 1045 3878 4200 4773 4984 6763 7898 8579
Size: 219 2833 322 573 211 1779 1135 681
Cuts at: 8579 8770 826
Size: 191 1471
Fragments arranged by size:
2833 1779 1471 1135 681 573 322 219
211 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 4474 4474
Size: 9415
SfoI GGC'GCC
Cuts at: 3338 4384 5458 6100 6405 3338
Size: 1046 1074 642 305 6348
Fragments arranged by size:
6348 1074 1046 642 305
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9415
SgrAI Cr'CCGG_yG
Cuts at: 1700 2265 1700
Size: 565 8850
SmaI CCC'GGG
Cuts at: 1872 2497 2690 5379 6093 6334 6428 6708 6742
Size: 625 193 2689 714 241 94 280 34
Cuts at: 6742 1872
Size: 4545
Fragments arranged by size:
4545 2689 714 625 280 241 193 94
34
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3497 4530 5126 5485 5786 5864
Size: 197 926 1554 1033 596 359 301 78
Cuts at: 5864 6621 7519 8420 8682 820
Size: 757 898 901 262 1553
Fragments arranged by size:
1554 1553 1033 926 901 898 757 596
359 301 262 197 78
SnaBI TAC'GTA
Cuts at: 493 7206 493
Size: 6713 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9415
SphI G_CATG'C
Cuts at: 1132 6773 1132
Size: 5641 3774
SspI AAT'ATT
Cuts at: 5 52 3400 7339 5
Size: 47 3348 3939 2081
Fragments arranged by size:
3939 3348 2081 47
StuI AGG'CCT
Cuts at: 1517 3260 4468 4708 6672 1517
Size: 1743 1208 240 1964 4260
Fragments arranged by size:
4260 1964 1743 1208 240
StyI C'CwwG_G
Cuts at: 513 1065 2173 2934 3525 4230 4631 5250 5452
Size: 552 1108 761 591 705 401 619 202
Cuts at: 5452 5899 5962 6228 6375 6854 513
Size: 447 63 266 147 479 3074
Fragments arranged by size:
3074 1108 761 705 619 591 552 479
447 401 266 202 147 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2149 4876 4958 5081 6433 6577 7442
Size: 1255 569 2727 82 123 1352 144 865
Cuts at: 7442 7459 7612 7797 8242 325
Size: 17 153 185 445 1498
Fragments arranged by size:
2727 1498 1352 1255 865 569 445 185
153 144 123 82 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3432 3660 7661
Size: 274 80 33 51 1443 1455 228 4001
Cuts at: 7661 9373 96
Size: 1712 138
Fragments arranged by size:
4001 1712 1455 1443 274 228 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2387 2419 4046 4078 4561 4593 5621 5653 9375
Size: 32 1627 32 483 32 1028 32 3722
Cuts at: 9375 9407 2387
Size: 32 2395
Fragments arranged by size:
3722 2395 1627 1028 483 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2704 1316
Size: 1388 8027
XbaI T'CTAG_A
Cuts at: 6751 6751
Size: 9415
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2578 3711 4375 6622 2578
Size: 1133 664 2247 5371
Fragments arranged by size:
5371 2247 1133 664
XhoI C'TCGA_G
Cuts at: 1943 5485 5864 1943
Size: 3542 379 5494
Fragments arranged by size:
5494 3542 379
XmaI C'CCGG_G
Cuts at: 1870 2495 2688 5377 6091 6332 6426 6706 6740
Size: 625 193 2689 714 241 94 280 34
Cuts at: 6740 1870
Size: 4545
Fragments arranged by size:
4545 2689 714 625 280 241 193 94
34
XmnI GAAnn'nnTTC
Cuts at: 3122 7544 3122
Size: 4422 4993
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6160 7223 276
Size: 53 83 186 936 4626 1063 2468
Fragments arranged by size:
4626 2468 1063 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SacII SalI SanDI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI BaeI BsiWI BstZ17I FseI FspAI NruI PacI
PsrI RsrII SapI SrfI SwaI