Restriction map for
pFN21AA0972
(Circular) (Six-base) MAPSORT of: pFN21AA0972.seq Check: 3104 from: 1 to: 6754
pFN21AA0972 6754 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2937 2937
Size: 6754
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3170 4564 278
Size: 53 83 186 936 1634 1394 2468
Fragments arranged by size:
2468 1634 1394 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4007 4097 1937
Size: 2070 90 4594
Fragments arranged by size:
4594 2070 90
Acc65I G'GTAC_C
Cuts at: 4075 4075
Size: 6754
AclI AA'CG_TT
Cuts at: 1563 4881 5254 1563
Size: 3318 373 3063
Fragments arranged by size:
3318 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4817 6201 6469 1632
Size: 3185 1384 268 1917
Fragments arranged by size:
3185 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6557
AflIII A'CryG_T
Cuts at: 1203 5653 6630 1203
Size: 4450 977 1327
Fragments arranged by size:
4450 1327 977
AgeI A'CCGG_T
Cuts at: 5585 5585
Size: 6754
AhdI GACnn_n'nnGTC
Cuts at: 5483 5483
Size: 6754
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6754
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2514 1184
Size: 1330 5424
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2303 2335 2303
Size: 32 6722
AlwNI CAG_nnn'CTG
Cuts at: 4497 6069 4497
Size: 1572 5182
ApaLI G'TGCA_C
Cuts at: 4811 5967 4811
Size: 1156 5598
ApoI r'AATT_y
Cuts at: 1493 2165 2333 2548 2567 2629 2675 2861 3360
Size: 672 168 215 19 62 46 186 499
Cuts at: 3360 3444 3528 3780 3827 3864 4029 4063 4289
Size: 84 84 252 47 37 165 34 226
Cuts at: 4289 4441 6550 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 672 499 252 226 215 186
168 165 152 84 84 62 47 46
37 34 19
AseI AT'TA_AT
Cuts at: 160 1032 2577 5308 160
Size: 872 1545 2731 1606
Fragments arranged by size:
2731 1606 1545 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6754
AvaI C'yCGr_G
Cuts at: 1870 1943 4079 1870
Size: 73 2136 4545
Fragments arranged by size:
4545 2136 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2254 2287 2254
Size: 33 6721
BamHI G'GATC_C
Cuts at: 4084 4451 4084
Size: 367 6387
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4075 5530 618
Size: 325 217 627 2288 1455 1842
Fragments arranged by size:
2288 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4073 1391
Size: 2682 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5783
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4945 4979 1903
Size: 34 3008 34 3678
Fragments arranged by size:
3678 3008 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4646 5856 4646
Size: 1210 5544
BclI T'GATC_A
Cuts at: 1548 2379 3853 1548
Size: 831 1474 4449
Fragments arranged by size:
4449 1474 831
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5365 136
Size: 107 122 71 631 605 3693 1525
Fragments arranged by size:
3693 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6749 6749
Size: 6754
BlpI GC'TnA_GC
Cuts at: 4171 4171
Size: 6754
Bme1580I G_kGCm'C
Cuts at: 1790 4815 5971 1790
Size: 3025 1156 2573
Fragments arranged by size:
3025 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2155 1318
Size: 837 5917
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3630 3798 4485
Size: 416 53 83 399 24 2213 168 687
Cuts at: 4485 5443 442
Size: 958 1753
Fragments arranged by size:
2213 1753 958 687 416 399 168 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6754
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2353 2353
Size: 6754
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2252 2688 5414 1239
Size: 1013 436 2726 2579
Fragments arranged by size:
2726 2579 1013 436
Bpu10I CC'TnA_GC
Cuts at: 4013 4013
Size: 6754
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4879 5744 6042 4879
Size: 865 298 5591
Fragments arranged by size:
5591 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5417 882
Size: 617 3918 2219
Fragments arranged by size:
3918 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4545 493
Size: 711 3341 2702
Fragments arranged by size:
3341 2702 711
BsaBI GATnn'nnATC
Cuts at: 6748 6748
Size: 6754
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3167 4561 4943 5578
Size: 53 83 186 936 1634 1394 382 635
Cuts at: 5578 275
Size: 1451
Fragments arranged by size:
1634 1451 1394 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3834 5187 5585 5859 6006 6417 3834
Size: 1353 398 274 147 411 4171
Fragments arranged by size:
4171 1353 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2303 2333 1178
Size: 30 165 30 900 30 5599
Fragments arranged by size:
5599 900 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2192 2324 2342 1186
Size: 1006 132 18 5598
Fragments arranged by size:
5598 1006 132 18
BseYI C'CCAG_C
Cuts at: 5957 6453 5957
Size: 496 6258
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6181
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4204 4965 5993 1540
Size: 275 186 2203 761 1028 2301
Fragments arranged by size:
2301 2203 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4815 4900 5971 4815
Size: 85 1071 5598
Fragments arranged by size:
5598 1071 85
BsmI GAATG_Cn'
Cuts at: 3980 4265 4358 3980
Size: 285 93 6376
Fragments arranged by size:
6376 285 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2257 2257
Size: 6754
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4073 4815 4900 5971 1391
Size: 399 2283 742 85 1071 2174
Fragments arranged by size:
2283 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 3834 6417 3834
Size: 2583 4171
BspHI T'CATG_A
Cuts at: 3142 4643 6373 3142
Size: 1501 1730 3523
Fragments arranged by size:
3523 1730 1501
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2937 4109 844
Size: 2093 1172 3489
Fragments arranged by size:
3489 2093 1172
BsrBI CCG'CTC
Cuts at: 1394 1823 4641 1394
Size: 429 2818 3507
Fragments arranged by size:
3507 2818 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3985 5249 5423 65
Size: 1183 2737 1264 174 1396
Fragments arranged by size:
2737 1396 1264 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5398 5585 1700
Size: 110 3588 187 2869
Fragments arranged by size:
3588 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2214 96
Size: 2118 4636
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6754
BssSI C'ACGA_G
Cuts at: 4814 5826 4814
Size: 1012 5742
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6754
BstBI TT'CG_AA
Cuts at: 5558 5558
Size: 6754
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6754
BstYI r'GATC_y
Cuts at: 1972 4084 4451 4836 4853 6305 6391 6403 6749
Size: 2112 367 385 17 1452 86 12 346
Cuts at: 6749 1972
Size: 1977
Fragments arranged by size:
2112 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3428 3428
Size: 6754
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6202
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6015
Fragments arranged by size:
6015 683 56
BtsI GCAGTG_nn'
Cuts at: 4270 5064 5084 4270
Size: 794 20 5940
Fragments arranged by size:
5940 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6754
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3547 3582 529
Size: 35 2983 35 3701
Fragments arranged by size:
3701 2983 35 35
DraI TTT'AAA
Cuts at: 3805 4058 3805
Size: 253 6501
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5761 809
Size: 507 4445 1802
Fragments arranged by size:
4445 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3580 3916 4201
Size: 54 1425 50 275 18 1750 336 285
Cuts at: 4201 5090 6719 8
Size: 889 1629 43
Fragments arranged by size:
1750 1629 1425 889 336 285 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4201 1537
Size: 275 2389 4090
Fragments arranged by size:
4090 2389 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2454 3078 4684 1353
Size: 1101 624 1606 3423
Fragments arranged by size:
3423 1606 1101 624
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5341 5715 5861 387
Size: 1064 138 3752 374 146 1280
Fragments arranged by size:
3752 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2252 2688 4817 5414 6201 6469 1239
Size: 393 620 436 2129 597 787 268 1524
Fragments arranged by size:
2129 1524 787 620 597 436 393 268
EcoRI G'AATT_C
Cuts at: 2165 2333 2629 3360 3444 3528 3780 3864 4063
Size: 168 296 731 84 84 252 84 199
Cuts at: 4063 4441 2165
Size: 378 4478
Fragments arranged by size:
4478 731 378 296 252 199 168 84
84 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3863 3863
Size: 6754
FspI TGC'GCA
Cuts at: 5260 5260
Size: 6754
HaeII r_GCGC'y
Cuts at: 1678 5901 1678
Size: 4223 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5395 5427 5469 5501 1373
Size: 32 135 32 3823 32 42 32 2626
Fragments arranged by size:
3823 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2967 3423 4098 4344 669
Size: 988 281 1029 456 675 246 3079
Fragments arranged by size:
3079 1029 988 675 456 281 246
HindIII A'AGCT_T
Cuts at: 748 2760 4015 748
Size: 2012 1255 3487
Fragments arranged by size:
3487 2012 1255
HpaI GTT'AAC
Cuts at: 4344 4344
Size: 6754
KpnI G_GTAC'C
Cuts at: 4079 4079
Size: 6754
MfeI C'AATT_G
Cuts at: 4353 4353
Size: 6754
MluI A'CGCG_T
Cuts at: 6630 6630
Size: 6754
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2277 5868 6052 1305
Size: 972 3591 184 2007
Fragments arranged by size:
3591 2007 972 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3582 3918 10
Size: 54 1425 343 1750 336 2846
Fragments arranged by size:
2846 1750 1425 343 336 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3301 3721 3805 4712 5071 5230 518
Size: 648 2135 420 84 907 359 159 2042
Fragments arranged by size:
2135 2042 907 648 420 359 159 84
MspA1I CmG'CkG
Cuts at: 1606 3074 4170 4847 5995 6240 1606
Size: 1468 1096 677 1148 245 2120
Fragments arranged by size:
2120 1468 1148 1096 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6202
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6754
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6754
NotI GC'GGCC_GC
Cuts at: 4201 4201
Size: 6754
NspI r_CATG'y
Cuts at: 1132 1229 2495 4112 5657 1132
Size: 97 1266 1617 1545 2229
Fragments arranged by size:
2229 1617 1545 1266 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6754
PciI A'CATG_T
Cuts at: 5653 5653
Size: 6754
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6754
PmeI GTTT'AAAC
Cuts at: 4058 4058
Size: 6754
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2303 2335 4823 4855 6336 6368 2303
Size: 32 2488 32 1481 32 2689
Fragments arranged by size:
2689 2488 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6754
PsiI TTA'TAA
Cuts at: 2715 4324 2715
Size: 1609 5145
PstI C_TGCA'G
Cuts at: 830 4106 830
Size: 3276 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6754
PvuII CAG'CTG
Cuts at: 3074 3074
Size: 6754
SalI G'TCGA_C
Cuts at: 1936 4096 1936
Size: 2160 4594
SbfI CC_TGCA'GG
Cuts at: 4106 4106
Size: 6754
ScaI AGT'ACT
Cuts at: 3349 5002 6714 3349
Size: 1653 1712 3389
Fragments arranged by size:
3389 1712 1653
SfcI C'TryA_G
Cuts at: 826 1045 2160 2239 2451 4102 5237 5918 6109
Size: 219 1115 79 212 1651 1135 681 191
Cuts at: 6109 826
Size: 1471
Fragments arranged by size:
1651 1471 1135 1115 681 219 212 191
79
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6754
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6754
SmaI CCC'GGG
Cuts at: 1872 4081 1872
Size: 2209 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4858 5759 6021 820
Size: 197 926 2915 901 262 1553
Fragments arranged by size:
2915 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4545 493
Size: 4052 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6754
SphI G_CATG'C
Cuts at: 1132 4112 1132
Size: 2980 3774
SspI AAT'ATT
Cuts at: 5 52 4678 5
Size: 47 4626 2081
Fragments arranged by size:
4626 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6754
StyI C'CwwG_G
Cuts at: 513 1065 2315 4193 513
Size: 552 1250 1878 3074
Fragments arranged by size:
3074 1878 1250 552
SwaI ATTT'AAAT
Cuts at: 3805 3805
Size: 6754
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2186 2367 4781 4798 4951 5136 5581
Size: 1255 606 181 2414 17 153 185 445
Cuts at: 5581 325
Size: 1498
Fragments arranged by size:
2414 1498 1255 606 445 185 181 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2214 3347 5000
Size: 274 80 33 51 1443 237 1133 1653
Cuts at: 5000 6712 96
Size: 1712 138
Fragments arranged by size:
1712 1653 1443 1133 274 237 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2860 2892 3940 3972 6714 6746 2860
Size: 32 1048 32 2742 32 2868
Fragments arranged by size:
2868 2742 1048 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6754
XbaI T'CTAG_A
Cuts at: 4090 4090
Size: 6754
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2906 2906
Size: 6754
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6754
XmaI C'CCGG_G
Cuts at: 1870 4079 1870
Size: 2209 4545
XmnI GAAnn'nnTTC
Cuts at: 2590 2724 3239 3716 4883 2590
Size: 134 515 477 1167 4461
Fragments arranged by size:
4461 1167 515 477 134
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3168 4562 276
Size: 53 83 186 936 1634 1394 2468
Fragments arranged by size:
2468 1634 1394 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbeI BbvCI BfrBI BsiWI
BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI