Restriction map for
pFN21AA0966
(Circular) (Six-base) MAPSORT of: pFN21AA0966.seq Check: 366 from: 1 to: 8101
pFN21AA0966 8101 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5911 278
Size: 53 83 186 936 4375 2468
Fragments arranged by size:
4375 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3138 5444 1937
Size: 1201 2306 4594
Fragments arranged by size:
4594 2306 1201
Acc65I G'GTAC_C
Cuts at: 5422 5422
Size: 8101
AclI AA'CG_TT
Cuts at: 1563 6228 6601 1563
Size: 4665 373 3063
Fragments arranged by size:
4665 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2417 2525 3200 3590 4157 4170 4545 4743
Size: 785 108 675 390 567 13 375 198
Cuts at: 4743 5166 6164 7548 7816 1632
Size: 423 998 1384 268 1917
Fragments arranged by size:
1917 1384 998 785 675 567 423 390
375 268 198 108 13
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7904
AflIII A'CryG_T
Cuts at: 1203 4554 7000 7977 1203
Size: 3351 2446 977 1327
Fragments arranged by size:
3351 2446 1327 977
AgeI A'CCGG_T
Cuts at: 6932 6932
Size: 8101
AhdI GACnn_n'nnGTC
Cuts at: 6830 6830
Size: 8101
AleI CACnn'nnGTG
Cuts at: 1166 3401 4517 1166
Size: 2235 1116 4750
Fragments arranged by size:
4750 2235 1116
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4100 5121 1184
Size: 2916 1021 4164
Fragments arranged by size:
4164 2916 1021
AlwNI CAG_nnn'CTG
Cuts at: 3445 4544 5324 5844 7416 3445
Size: 1099 780 520 1572 4130
Fragments arranged by size:
4130 1572 1099 780 520
ApaI G_GGCC'C
Cuts at: 3871 3871
Size: 8101
ApaLI G'TGCA_C
Cuts at: 6158 7314 6158
Size: 1156 6945
ApoI r'AATT_y
Cuts at: 1493 2166 4327 4343 4589 4600 5410 5636 5788
Size: 673 2161 16 246 11 810 226 152
Cuts at: 5788 7897 1493
Size: 2109 1697
Fragments arranged by size:
2161 2109 1697 810 673 246 226 152
16 11
AseI AT'TA_AT
Cuts at: 160 1032 5334 6655 160
Size: 872 4302 1321 1606
Fragments arranged by size:
4302 1606 1321 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8101
AvaI C'yCGr_G
Cuts at: 1870 1943 5426 1870
Size: 73 3483 4545
Fragments arranged by size:
4545 3483 73
BamHI G'GATC_C
Cuts at: 5431 5798 5431
Size: 367 7734
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5422 6877 618
Size: 325 217 627 3635 1455 1842
Fragments arranged by size:
3635 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2013 2322 3871 5420 1391
Size: 622 309 1549 1549 4072
Fragments arranged by size:
4072 1549 1549 622 309
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2405 4281 4551 4623 928
Size: 971 506 1876 270 72 4406
Fragments arranged by size:
4406 1876 971 506 270 72
BbvCI CC'TCA_GC
Cuts at: 4693 4693
Size: 8101
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6292 6326 1903
Size: 34 4355 34 3678
Fragments arranged by size:
4355 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5993 7203 5993
Size: 1210 6891
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8101
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2079 2608 6712
Size: 107 122 71 631 605 407 529 4104
Cuts at: 6712 136
Size: 1525
Fragments arranged by size:
4104 1525 631 605 529 407 122 107
71
BglII A'GATC_T
Cuts at: 2182 2656 3721 4937 8096 2182
Size: 474 1065 1216 3159 2187
Fragments arranged by size:
3159 2187 1216 1065 474
BlpI GC'TnA_GC
Cuts at: 5518 5518
Size: 8101
Bme1580I G_kGCm'C
Cuts at: 1790 3085 3871 6162 7318 1790
Size: 1295 786 2291 1156 2573
Fragments arranged by size:
2573 2291 1295 1156 786
BmgBI CAC'GTC
Cuts at: 1318 3916 1318
Size: 2598 5503
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2592 4714 5832
Size: 416 53 83 399 24 1175 2122 1118
Cuts at: 5832 6790 442
Size: 958 1753
Fragments arranged by size:
2122 1753 1175 1118 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 4631 1056
Size: 3575 4526
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2072 2661 5301 6761 1239
Size: 833 589 2640 1460 2579
Fragments arranged by size:
2640 2579 1460 833 589
Bpu10I CC'TnA_GC
Cuts at: 4693 4693
Size: 8101
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2330 4268 5171 6226 7091 7389 2330
Size: 1938 903 1055 865 298 3042
Fragments arranged by size:
3042 1938 1055 903 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2235 2777 4299 4925 6764 882
Size: 617 736 542 1522 626 1839 2219
Fragments arranged by size:
2219 1839 1522 736 626 617 542
BsaAI yAC'GTr
Cuts at: 493 1204 3955 5892 493
Size: 711 2751 1937 2702
Fragments arranged by size:
2751 2702 1937 711
BsaBI GATnn'nnATC
Cuts at: 8095 8095
Size: 8101
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5908 6290 6925 275
Size: 53 83 186 936 4375 382 635 1451
Fragments arranged by size:
4375 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6534 6932 7206 7353 7764 6534
Size: 398 274 147 411 6871
Fragments arranged by size:
6871 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5161 5191 1178
Size: 30 165 30 3758 30 4088
Fragments arranged by size:
4088 3758 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2497 2907 1186
Size: 1311 410 6380
Fragments arranged by size:
6380 1311 410
BseYI C'CCAG_C
Cuts at: 3335 3554 3864 7304 7800 3335
Size: 219 310 3440 496 3636
Fragments arranged by size:
3636 3440 496 310 219
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2086 2588 2741 4070 1163
Size: 573 350 502 153 1329 5194
Fragments arranged by size:
5194 1329 573 502 350 153
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2599 5551 6312 7340 1540
Size: 275 186 598 2952 761 1028 2301
Fragments arranged by size:
2952 2301 1028 761 598 275 186
BsiHKAI G_wGCw'C
Cuts at: 2013 2280 2322 2582 3243 6162 6247 7318 2013
Size: 267 42 260 661 2919 85 1071 2796
Fragments arranged by size:
2919 2796 1071 661 267 260 85 42
BsmI GAATG_Cn'
Cuts at: 2880 3705 5098 5612 5705 2880
Size: 825 1393 514 93 5276
Fragments arranged by size:
5276 1393 825 514 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3906 3906
Size: 8101
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2280 2322 2582 3085 3243 3871
Size: 399 223 267 42 260 503 158 628
Cuts at: 3871 5420 6162 6247 7318 1391
Size: 1549 742 85 1071 2174
Fragments arranged by size:
2174 1549 1071 742 628 503 399 267
260 223 158 85 42
BspEI T'CCGG_A
Cuts at: 7764 7764
Size: 8101
BspHI T'CATG_A
Cuts at: 5990 7720 5990
Size: 1730 6371
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5456 844
Size: 4612 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5988 1394
Size: 429 4165 3507
Fragments arranged by size:
4165 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4124 4179 6596 6770 65
Size: 1183 2876 55 2417 174 1396
Fragments arranged by size:
2876 2417 1396 1183 174 55
BsrFI r'CCGG_y
Cuts at: 1700 1810 6745 6932 1700
Size: 110 4935 187 2869
Fragments arranged by size:
4935 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8101
BssHII G'CGCG_C
Cuts at: 1925 2053 2055 1925
Size: 128 2 7971
Fragments arranged by size:
7971 128 2
BssSI C'ACGA_G
Cuts at: 2982 6161 7173 2982
Size: 3179 1012 3910
Fragments arranged by size:
3910 3179 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3190 794
Size: 2396 5705
BstBI TT'CG_AA
Cuts at: 3090 5227 6905 3090
Size: 2137 1678 4286
Fragments arranged by size:
4286 2137 1678
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8101
BstXI CCAn_nnnn'nTGG
Cuts at: 2137 2926 4870 2137
Size: 789 1944 5368
Fragments arranged by size:
5368 1944 789
BstYI r'GATC_y
Cuts at: 1972 2182 2305 2656 3721 4937 5431 5798 6183
Size: 210 123 351 1065 1216 494 367 385
Cuts at: 6183 6200 7652 7738 7750 8096 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1216 1065 494 385 367 351
346 210 123 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2301 2793 2301
Size: 492 7609
BtgI C'CryG_G
Cuts at: 513 1065 2004 2189 3038 3856 4212 5235 5275
Size: 552 939 185 849 818 356 1023 40
Cuts at: 5275 513
Size: 3339
Fragments arranged by size:
3339 1023 939 849 818 552 356 185
40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3183 3403 5022 487
Size: 683 56 1957 220 1619 3566
Fragments arranged by size:
3566 1957 1619 683 220 56
BtsI GCAGTG_nn'
Cuts at: 3241 5298 5617 6411 6431 3241
Size: 2057 319 794 20 4911
Fragments arranged by size:
4911 2057 794 319 20
ClaI AT'CG_AT
Cuts at: 1554 3022 1554
Size: 1468 6633
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4036 4071 5061 5096 529
Size: 35 3472 35 990 35 3534
Fragments arranged by size:
3534 3472 990 35 35 35
DraI TTT'AAA
Cuts at: 5405 5405
Size: 8101
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2249 7108 809
Size: 507 933 4859 1802
Fragments arranged by size:
4859 1802 933 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5033 5548 6437
Size: 54 1425 50 275 18 3203 515 889
Cuts at: 6437 8066 8
Size: 1629 43
Fragments arranged by size:
3203 1629 1425 889 515 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5548 1537
Size: 275 3736 4090
Fragments arranged by size:
4090 3736 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2018 2490 2884 3145 4146 4250 5303 6031
Size: 665 472 394 261 1001 104 1053 728
Cuts at: 6031 1353
Size: 3423
Fragments arranged by size:
3423 1053 1001 728 665 472 394 261
104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6688 7062 7208 387
Size: 1064 138 5099 374 146 1280
Fragments arranged by size:
5099 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2011 2320 2011
Size: 309 7792
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2417 2525 2661 3200 3590 4157
Size: 393 440 345 108 136 539 390 567
Cuts at: 4157 4170 4545 4743 5166 5301 6164 6761 7548
Size: 13 375 198 423 135 863 597 787
Cuts at: 7548 7816 1239
Size: 268 1524
Fragments arranged by size:
1524 863 787 597 567 539 440 423
393 390 375 345 268 198 136 135
108 13
EcoNI CCTnn'n_nnAGG
Cuts at: 2042 2042
Size: 8101
EcoO109I rG'GnC_Cy
Cuts at: 3868 4037 3868
Size: 169 7932
EcoRI G'AATT_C
Cuts at: 4589 5410 5788 4589
Size: 821 378 6902
Fragments arranged by size:
6902 821 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3209 3595 4187 4490 3209
Size: 386 592 303 6820
Fragments arranged by size:
6820 592 386 303
FspI TGC'GCA
Cuts at: 6607 6607
Size: 8101
HaeII r_GCGC'y
Cuts at: 1678 4700 7248 1678
Size: 3022 2548 2531
Fragments arranged by size:
3022 2548 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2510 2542 2581 2613 2641
Size: 32 135 32 938 32 39 32 28
Cuts at: 2641 2659 2673 2691 2776 2808 3550 3582 3636
Size: 18 14 18 85 32 742 32 54
Cuts at: 3636 3668 3706 3738 4556 4588 4922 4954 5014
Size: 32 38 32 818 32 334 32 60
Cuts at: 5014 5046 6742 6774 6816 6848 1373
Size: 32 1696 32 42 32 2626
Fragments arranged by size:
2626 1696 938 818 742 334 135 85
60 54 42 39 38 32 32 32
32 32 32 32 32 32 32 32
32 32 28 18 18 14
HincII GTy'rAC
Cuts at: 669 1657 1938 4444 5445 5691 669
Size: 988 281 2506 1001 246 3079
Fragments arranged by size:
3079 2506 1001 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3759 748
Size: 3011 5090
HpaI GTT'AAC
Cuts at: 4444 5691 4444
Size: 1247 6854
KpnI G_GTAC'C
Cuts at: 5426 5426
Size: 8101
MfeI C'AATT_G
Cuts at: 5700 5700
Size: 8101
MluI A'CGCG_T
Cuts at: 7977 7977
Size: 8101
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2119 2457 7215 7399 1305
Size: 814 338 4758 184 2007
Fragments arranged by size:
4758 2007 814 338 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6279
Fragments arranged by size:
6279 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3401 3710 4517 4584 4782 6059 6418
Size: 648 2235 309 807 67 198 1277 359
Cuts at: 6418 6577 518
Size: 159 2042
Fragments arranged by size:
2235 2042 1277 807 648 359 309 198
159 67
MspA1I CmG'CkG
Cuts at: 1606 3040 3445 3563 5517 6194 7342 7587 1606
Size: 1434 405 118 1954 677 1148 245 2120
Fragments arranged by size:
2120 1954 1434 1148 677 405 245 118
NcoI C'CATG_G
Cuts at: 513 1065 2004 2189 3856 4212 5235 5275 513
Size: 552 939 185 1667 356 1023 40 3339
Fragments arranged by size:
3339 1667 1023 939 552 356 185 40
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8101
NheI G'CTAG_C
Cuts at: 1052 4627 1052
Size: 3575 4526
NotI GC'GGCC_GC
Cuts at: 5548 5548
Size: 8101
NruI TCG'CGA
Cuts at: 3422 3422
Size: 8101
NspI r_CATG'y
Cuts at: 1132 1229 4081 4558 5143 5459 7004 1132
Size: 97 2852 477 585 316 1545 2229
Fragments arranged by size:
2852 2229 1545 585 477 316 97
PasI CC'CwG_GG
Cuts at: 1348 4318 1348
Size: 2970 5131
PciI A'CATG_T
Cuts at: 4554 7000 4554
Size: 2446 5655
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8101
PmeI GTTT'AAAC
Cuts at: 5405 5405
Size: 8101
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6170 6202 7683 7715 6170
Size: 32 1481 32 6556
Fragments arranged by size:
6556 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8101
PsiI TTA'TAA
Cuts at: 5671 5671
Size: 8101
PspOMI G'GGCC_C
Cuts at: 3867 3867
Size: 8101
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3465 3497 3465
Size: 32 8069
PstI C_TGCA'G
Cuts at: 830 2043 4197 5453 830
Size: 1213 2154 1256 3478
Fragments arranged by size:
3478 2154 1256 1213
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8101
PvuII CAG'CTG
Cuts at: 3445 3563 3445
Size: 118 7983
SacI G_AGCT'C
Cuts at: 2013 2322 2013
Size: 309 7792
SacII CC_GC'GG
Cuts at: 3041 3041
Size: 8101
SalI G'TCGA_C
Cuts at: 1936 5443 1936
Size: 3507 4594
SapI GCTCTTCn'nnn_
Cuts at: 2018 4250 2018
Size: 2232 5869
SbfI CC_TGCA'GG
Cuts at: 5453 5453
Size: 8101
ScaI AGT'ACT
Cuts at: 6349 8061 6349
Size: 1712 6389
SfcI C'TryA_G
Cuts at: 826 1045 2039 4090 4193 5449 6584 7265 7456
Size: 219 994 2051 103 1256 1135 681 191
Cuts at: 7456 826
Size: 1471
Fragments arranged by size:
2051 1471 1256 1135 994 681 219 191
103
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8101
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8101
SmaI CCC'GGG
Cuts at: 1872 5428 1872
Size: 3556 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2309 4247 5150 6205 7106 7368
Size: 197 926 366 1938 903 1055 901 262
Cuts at: 7368 820
Size: 1553
Fragments arranged by size:
1938 1553 1055 926 903 901 366 262
197
SnaBI TAC'GTA
Cuts at: 493 5892 493
Size: 5399 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8101
SphI G_CATG'C
Cuts at: 1132 4081 5143 5459 1132
Size: 2949 1062 316 3774
Fragments arranged by size:
3774 2949 1062 316
SspI AAT'ATT
Cuts at: 5 52 4393 6025 5
Size: 47 4341 1632 2081
Fragments arranged by size:
4341 2081 1632 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8101
StyI C'CwwG_G
Cuts at: 513 1065 2004 2022 2189 2710 3741 3856 4212
Size: 552 939 18 167 521 1031 115 356
Cuts at: 4212 4303 5235 5275 5540 513
Size: 91 932 40 265 3074
Fragments arranged by size:
3074 1031 939 932 552 521 356 265
167 115 91 40 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2640 2838 4704 6128 6145 6298 6483
Size: 1255 1060 198 1866 1424 17 153 185
Cuts at: 6483 6928 325
Size: 445 1498
Fragments arranged by size:
1866 1498 1424 1255 1060 445 198 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6347 8059 96
Size: 274 80 33 51 1443 4370 1712 138
Fragments arranged by size:
4370 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3352 3384 8061 8093 3352
Size: 32 4677 32 3360
Fragments arranged by size:
4677 3360 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4005 1316
Size: 2689 5412
XbaI T'CTAG_A
Cuts at: 4009 5437 4009
Size: 1428 6673
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3424 4210 3424
Size: 786 7315
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8101
XmaI C'CCGG_G
Cuts at: 1870 5426 1870
Size: 3556 4545
XmnI GAAnn'nnTTC
Cuts at: 2510 6230 2510
Size: 3720 4381
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5909 276
Size: 53 83 186 936 4375 2468
Fragments arranged by size:
4375 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NruI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SacI SacII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NsiI PacI PfoI PmlI PpuMI
PspXI RsrII SanDI SexAI SfiI SfoI SrfI SwaI