Restriction map for
pFN21AA0954
(Circular) (Six-base) MAPSORT of: pFN21AA0954.seq Check: 8658 from: 1 to: 8158
pFN21AA0954 8158 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2446 2446
Size: 8158
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5968 278
Size: 53 83 186 936 4432 2468
Fragments arranged by size:
4432 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4398 5501 1937
Size: 2461 1103 4594
Fragments arranged by size:
4594 2461 1103
Acc65I G'GTAC_C
Cuts at: 5479 5479
Size: 8158
AclI AA'CG_TT
Cuts at: 1563 6285 6658 1563
Size: 4722 373 3063
Fragments arranged by size:
4722 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3317 3430 4159 4321 6221 7605 7873 1632
Size: 1685 113 729 162 1900 1384 268 1917
Fragments arranged by size:
1917 1900 1685 1384 729 268 162 113
AfeI AGC'GCT
Cuts at: 2039 4106 4292 2039
Size: 2067 186 5905
Fragments arranged by size:
5905 2067 186
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7961
AflIII A'CryG_T
Cuts at: 1203 3784 4629 7057 8034 1203
Size: 2581 845 2428 977 1327
Fragments arranged by size:
2581 2428 1327 977 845
AgeI A'CCGG_T
Cuts at: 5316 6989 5316
Size: 1673 6485
AhdI GACnn_n'nnGTC
Cuts at: 6887 6887
Size: 8158
AleI CACnn'nnGTG
Cuts at: 1166 3422 1166
Size: 2256 5902
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3605 5186 1184
Size: 2421 1581 4156
Fragments arranged by size:
4156 2421 1581
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5189 5221 5189
Size: 32 8126
AlwNI CAG_nnn'CTG
Cuts at: 2671 2713 2725 2815 4610 5068 5901 7473 2671
Size: 42 12 90 1795 458 833 1572 3356
Fragments arranged by size:
3356 1795 1572 833 458 90 42 12
ApaI G_GGCC'C
Cuts at: 2364 2924 4957 4969 4988 5008 2364
Size: 560 2033 12 19 20 5514
Fragments arranged by size:
5514 2033 560 20 19 12
ApaLI G'TGCA_C
Cuts at: 3722 3842 6215 7371 3722
Size: 120 2373 1156 4509
Fragments arranged by size:
4509 2373 1156 120
ApoI r'AATT_y
Cuts at: 1493 5074 5348 5467 5693 5845 7954 1493
Size: 3581 274 119 226 152 2109 1697
Fragments arranged by size:
3581 2109 1697 274 226 152 119
AseI AT'TA_AT
Cuts at: 160 1032 6712 160
Size: 872 5680 1606
Fragments arranged by size:
5680 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8158
AvaI C'yCGr_G
Cuts at: 1870 1943 2254 2281 2347 2428 2874 2917 3263
Size: 73 311 27 66 81 446 43 346
Cuts at: 3263 3860 4165 5483 1870
Size: 597 305 1318 4545
Fragments arranged by size:
4545 1318 597 446 346 311 305 81
73 66 43 27
BamHI G'GATC_C
Cuts at: 5488 5855 5488
Size: 367 7791
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2066 2259 2291 2354 2674
Size: 325 217 627 279 193 32 63 320
Cuts at: 2674 2970 2981 3652 4169 5162 5306 5479 6934
Size: 296 11 671 517 993 144 173 1455
Cuts at: 6934 618
Size: 1842
Fragments arranged by size:
1842 1455 993 671 627 517 325 320
296 279 217 193 173 144 63 32
11
BanII G_rGCy'C
Cuts at: 1391 2079 2234 2364 2382 2435 2469 2637 2748
Size: 688 155 130 18 53 34 168 111
Cuts at: 2748 2881 2924 3246 3749 4957 4969 4988 4998
Size: 133 43 322 503 1208 12 19 10
Cuts at: 4998 5008 5477 1391
Size: 10 469 4072
Fragments arranged by size:
4072 1208 688 503 469 322 168 155
133 130 111 53 43 34 19 18
12 10 10
BbeI G_GCGC'C
Cuts at: 2263 4173 2263
Size: 1910 6248
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3658 928
Size: 971 1759 5428
Fragments arranged by size:
5428 1759 971
BbvCI CC'TCA_GC
Cuts at: 5177 5177
Size: 8158
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2855 2889 6349 6383 1903
Size: 34 918 34 3460 34 3678
Fragments arranged by size:
3678 3460 918 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6050 7260 6050
Size: 1210 6948
BclI T'GATC_A
Cuts at: 1548 2826 1548
Size: 1278 6880
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2171 2893 3024
Size: 107 122 71 631 605 499 722 131
Cuts at: 3024 3530 3684 6769 136
Size: 506 154 3085 1525
Fragments arranged by size:
3085 1525 722 631 605 506 499 154
131 122 107 71
BglII A'GATC_T
Cuts at: 3570 3933 8153 3570
Size: 363 4220 3575
Fragments arranged by size:
4220 3575 363
BlpI GC'TnA_GC
Cuts at: 2376 2742 4370 4992 5575 2376
Size: 366 1628 622 583 4959
Fragments arranged by size:
4959 1628 622 583 366
Bme1580I G_kGCm'C
Cuts at: 1790 2071 2357 2364 2520 2679 2924 2986 3649
Size: 281 286 7 156 159 245 62 663
Cuts at: 3649 3657 3726 3846 4487 4957 4969 4988 5008
Size: 8 69 120 641 470 12 19 20
Cuts at: 5008 5046 5309 6219 7375 1790
Size: 38 263 910 1156 2573
Fragments arranged by size:
2573 1156 910 663 641 470 286 281
263 245 159 156 120 69 62 38
20 19 12 8 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8158
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3349 5089 5889
Size: 416 53 83 399 24 1932 1740 800
Cuts at: 5889 6847 442
Size: 958 1753
Fragments arranged by size:
1932 1753 1740 958 800 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8158
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2146 2461 2762 4985 2146
Size: 315 301 2223 5319
Fragments arranged by size:
5319 2223 315 301
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2093 2123 2145 2194 2532 2549 3338 3512
Size: 854 30 22 49 338 17 789 174
Cuts at: 3512 4100 4121 4129 4889 4955 5090 5091 6818
Size: 588 21 8 760 66 135 1 1727
Cuts at: 6818 1239
Size: 2579
Fragments arranged by size:
2579 1727 854 789 760 588 338 174
135 66 49 30 22 21 17 8
1
Bpu10I CC'TnA_GC
Cuts at: 2228 3408 3918 4765 5177 2228
Size: 1180 510 847 412 5209
Fragments arranged by size:
5209 1180 847 510 412
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3257 4133 6283 7148 7446 3257
Size: 876 2150 865 298 3969
Fragments arranged by size:
3969 2150 876 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3446 6821 882
Size: 617 1947 3375 2219
Fragments arranged by size:
3375 2219 1947 617
BsaAI yAC'GTr
Cuts at: 493 1204 2509 3787 4366 5949 493
Size: 711 1305 1278 579 1583 2702
Fragments arranged by size:
2702 1583 1305 1278 711 579
BsaBI GATnn'nnATC
Cuts at: 5084 8152 5084
Size: 3068 5090
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2260 3180 3260 4170
Size: 53 83 186 936 727 920 80 910
Cuts at: 4170 5965 6347 6982 275
Size: 1795 382 635 1451
Fragments arranged by size:
1795 1451 936 920 910 727 635 382
186 83 80 53
BsaWI w'CCGG_w
Cuts at: 3363 3959 4267 5316 6591 6989 7263 7410 7821
Size: 596 308 1049 1275 398 274 147 411
Cuts at: 7821 3363
Size: 3700
Fragments arranged by size:
3700 1275 1049 596 411 398 308 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2417 2447 3954 3984 5189
Size: 30 165 30 1014 30 1507 30 1205
Cuts at: 5189 5219 5265 5295 1178
Size: 30 46 30 4041
Fragments arranged by size:
4041 1507 1205 1014 165 46 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2120 4400 5191 5194 5273 1186
Size: 934 2280 791 3 79 4071
Fragments arranged by size:
4071 2280 934 791 79 3
BseYI C'CCAG_C
Cuts at: 2451 2697 2792 3235 4455 4709 4745 4751 4772
Size: 246 95 443 1220 254 36 6 21
Cuts at: 4772 4931 5377 5412 7361 7857 2451
Size: 159 446 35 1949 496 2752
Fragments arranged by size:
2752 1949 1220 496 446 443 254 246
159 95 36 35 21 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2699 2849 3287 3425 3809 5156 5248
Size: 573 963 150 438 138 384 1347 92
Cuts at: 5248 1163
Size: 4073
Fragments arranged by size:
4073 1347 963 573 438 384 150 138
92
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3679 3704 5608 6369 7397 1540
Size: 275 186 1678 25 1904 761 1028 2301
Fragments arranged by size:
2301 1904 1678 1028 761 275 186 25
BsiHKAI G_wGCw'C
Cuts at: 2748 3726 3846 5107 6219 6304 7375 2748
Size: 978 120 1261 1112 85 1071 3531
Fragments arranged by size:
3531 1261 1112 1071 978 120 85
BsmI GAATG_Cn'
Cuts at: 5669 5762 5669
Size: 93 8065
BsmBI CGTCTCn'nnnn_
Cuts at: 2015 4076 2015
Size: 2061 6097
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2071 2079 2234 2357 2364 2382 2435
Size: 399 281 8 155 123 7 18 53
Cuts at: 2435 2469 2520 2637 2679 2748 2881 2924 2986
Size: 34 51 117 42 69 133 43 62
Cuts at: 2986 3246 3649 3657 3726 3749 3846 4487 4957
Size: 260 403 8 69 23 97 641 470
Cuts at: 4957 4969 4988 4998 5008 5046 5107 5309 5477
Size: 12 19 10 10 38 61 202 168
Cuts at: 5477 6219 6304 7375 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 641 470 403 399 281
260 202 168 155 133 123 117 97
85 69 69 62 61 53 51 43
42 38 34 23 19 18 12 10
10 8 8 7
BspEI T'CCGG_A
Cuts at: 4267 7821 4267
Size: 3554 4604
BspHI T'CATG_A
Cuts at: 6047 7777 6047
Size: 1730 6428
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2446 3403 3777 5513 844
Size: 1602 957 374 1736 3489
Fragments arranged by size:
3489 1736 1602 957 374
BsrBI CCG'CTC
Cuts at: 1394 1823 2911 4764 6045 1394
Size: 429 1088 1853 1281 3507
Fragments arranged by size:
3507 1853 1281 1088 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2943 4311 6653 6827 65
Size: 1183 1695 1368 2342 174 1396
Fragments arranged by size:
2342 1695 1396 1368 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3699 4157 5316 6802 6989 1700
Size: 110 1889 458 1159 1486 187 2869
Fragments arranged by size:
2869 1889 1486 1159 458 187 110
BsrGI T'GTAC_A
Cuts at: 96 2619 3222 96
Size: 2523 603 5032
Fragments arranged by size:
5032 2523 603
BssHII G'CGCG_C
Cuts at: 1925 3772 1925
Size: 1847 6311
BssSI C'ACGA_G
Cuts at: 6218 7230 6218
Size: 1012 7146
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8158
BstBI TT'CG_AA
Cuts at: 2046 6962 2046
Size: 4916 3242
BstEII G'GTnAC_C
Cuts at: 1184 4576 1184
Size: 3392 4766
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2101 4463 4505 4780 2011
Size: 90 2362 42 275 5389
Fragments arranged by size:
5389 2362 275 90 42
BstYI r'GATC_y
Cuts at: 1972 2614 3570 3822 3933 5488 5855 6240 6257
Size: 642 956 252 111 1555 367 385 17
Cuts at: 6257 7709 7795 7807 8153 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1555 1452 956 642 385 367 346
252 111 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3942 5234 3942
Size: 1292 6866
BtgI C'CryG_G
Cuts at: 513 1065 2004 2185 2263 2658 3673 4260 4487
Size: 552 939 181 78 395 1015 587 227
Cuts at: 4487 4539 4945 513
Size: 52 406 3726
Fragments arranged by size:
3726 1015 939 587 552 406 395 227
181 78 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2213 2868 3642 3898 487
Size: 683 56 987 655 774 256 4747
Fragments arranged by size:
4747 987 774 683 655 256 56
BtsI GCAGTG_nn'
Cuts at: 2405 5168 5372 5674 6468 6488 2405
Size: 2763 204 302 794 20 4075
Fragments arranged by size:
4075 2763 794 302 204 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8158
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8123
DraI TTT'AAA
Cuts at: 5462 5462
Size: 8158
DraIII CAC_nnn'GTG
Cuts at: 3310 3310
Size: 8158
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7165 809
Size: 507 5849 1802
Fragments arranged by size:
5849 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3561 3676 3701
Size: 54 1425 50 275 18 1731 115 25
Cuts at: 3701 4122 4242 4723 4843 4878 4948 5321 5605
Size: 421 120 481 120 35 70 373 284
Cuts at: 5605 6494 8123 8
Size: 889 1629 43
Fragments arranged by size:
1731 1629 1425 889 481 421 373 284
275 120 120 115 70 54 50 43
35 25 18
EagI C'GGCC_G
Cuts at: 1537 1812 3676 3701 5605 1537
Size: 275 1864 25 1904 4090
Fragments arranged by size:
4090 1904 1864 275 25
EarI CTCTTCn'nnn_
Cuts at: 1353 2762 3812 4220 6088 1353
Size: 1409 1050 408 1868 3423
Fragments arranged by size:
3423 1868 1409 1050 408
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2526 6745 7119 7265 387
Size: 1064 138 937 4219 374 146 1280
Fragments arranged by size:
4219 1280 1064 937 374 146 138
EcoICRI GAG'CTC
Cuts at: 2746 2746
Size: 8158
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2123 2145 2194 2532 2549 3317
Size: 393 461 30 22 49 338 17 768
Cuts at: 3317 3338 3430 3512 4100 4121 4129 4159 4321
Size: 21 92 82 588 21 8 30 162
Cuts at: 4321 4889 4955 5090 5091 6221 6818 7605 7873
Size: 568 66 135 1 1130 597 787 268
Cuts at: 7873 1239
Size: 1524
Fragments arranged by size:
1524 1130 787 768 597 588 568 461
393 338 268 162 135 92 82 66
49 30 30 22 21 21 17 8
1
EcoNI CCTnn'n_nnAGG
Cuts at: 3002 3002
Size: 8158
EcoO109I rG'GnC_Cy
Cuts at: 2361 2564 2921 3268 3322 4230 4390 4769 4965
Size: 203 357 347 54 908 160 379 196
Cuts at: 4965 4966 4985 2361
Size: 1 19 5534
Fragments arranged by size:
5534 908 379 357 347 203 196 160
54 19 1
EcoRI G'AATT_C
Cuts at: 5074 5348 5467 5845 5074
Size: 274 119 378 7387
Fragments arranged by size:
7387 378 274 119
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3223 3394 3952 3223
Size: 171 558 7429
Fragments arranged by size:
7429 558 171
FspI TGC'GCA
Cuts at: 2480 4379 6664 2480
Size: 1899 2285 3974
Fragments arranged by size:
3974 2285 1899
HaeII r_GCGC'y
Cuts at: 1678 2033 2041 2263 2331 3716 4108 4173 4294
Size: 355 8 222 68 1385 392 65 121
Cuts at: 4294 7305 1678
Size: 3011 2531
Fragments arranged by size:
3011 2531 1385 392 355 222 121 68
65 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2417 2449 3954 3986 6799
Size: 32 135 32 845 32 1505 32 2813
Cuts at: 6799 6831 6873 6905 1373
Size: 32 42 32 2626
Fragments arranged by size:
2813 2626 1505 845 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5502 5748 669
Size: 988 281 3564 246 3079
Fragments arranged by size:
3564 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8158
HpaI GTT'AAC
Cuts at: 5748 5748
Size: 8158
KasI G'GCGC_C
Cuts at: 2259 4169 2259
Size: 1910 6248
KpnI G_GTAC'C
Cuts at: 5483 5483
Size: 8158
MfeI C'AATT_G
Cuts at: 3812 5757 3812
Size: 1945 6213
MluI A'CGCG_T
Cuts at: 8034 8034
Size: 8158
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2106 2157 3624 3789 4546 7272 7456 1305
Size: 801 51 1467 165 757 2726 184 2007
Fragments arranged by size:
2726 2007 1467 801 757 184 165 51
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4124 4244 4725 4845 4880
Size: 54 1425 343 2292 120 481 120 35
Cuts at: 4880 5323 10
Size: 443 2845
Fragments arranged by size:
2845 2292 1425 481 443 343 120 120
54 35
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3230 3422 3515 3587 3729 4028
Size: 648 843 1221 192 93 72 142 299
Cuts at: 4028 5063 6116 6475 6634 518
Size: 1035 1053 359 159 2042
Fragments arranged by size:
2042 1221 1053 1035 843 648 359 299
192 159 142 93 72
MspA1I CmG'CkG
Cuts at: 1606 2214 2299 2611 3643 5574 6251 7399 7644
Size: 608 85 312 1032 1931 677 1148 245
Cuts at: 7644 1606
Size: 2120
Fragments arranged by size:
2120 1931 1148 1032 677 608 312 245
85
NarI GG'CG_CC
Cuts at: 2260 4170 2260
Size: 1910 6248
NcoI C'CATG_G
Cuts at: 513 1065 2004 2263 2658 4487 4945 513
Size: 552 939 259 395 1829 458 3726
Fragments arranged by size:
3726 1829 939 552 458 395 259
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8158
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8158
NotI GC'GGCC_GC
Cuts at: 5605 5605
Size: 8158
NspI r_CATG'y
Cuts at: 1132 1229 3235 3472 3835 4633 5516 7061 1132
Size: 97 2006 237 363 798 883 1545 2229
Fragments arranged by size:
2229 2006 1545 883 798 363 237 97
PasI CC'CwG_GG
Cuts at: 1348 2237 2902 4463 4901 5008 5302 1348
Size: 889 665 1561 438 107 294 4204
Fragments arranged by size:
4204 1561 889 665 438 294 107
PciI A'CATG_T
Cuts at: 4629 7057 4629
Size: 2428 5730
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3060 4120 4818 5068 1108
Size: 1952 1060 698 250 4198
Fragments arranged by size:
4198 1952 1060 698 250
PfoI T'CCnGG_A
Cuts at: 2100 2100
Size: 8158
PmeI GTTT'AAAC
Cuts at: 5462 5462
Size: 8158
PmlI CAC'GTG
Cuts at: 2509 3787 2509
Size: 1278 6880
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5189 5221 6227 6259 7740 7772 5189
Size: 32 1006 32 1481 32 5575
Fragments arranged by size:
5575 1481 1006 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4390 4390
Size: 8158
PshAI GACnn'nnGTC
Cuts at: 1613 3908 1613
Size: 2295 5863
PsiI TTA'TAA
Cuts at: 5728 5728
Size: 8158
PspOMI G'GGCC_C
Cuts at: 2360 2920 4953 4965 4984 5004 2360
Size: 560 2033 12 19 20 5514
Fragments arranged by size:
5514 2033 560 20 19 12
PspXI vC'TCGA_Gb
Cuts at: 2874 2874
Size: 8158
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3536 3568 5123 5155 3536
Size: 32 1555 32 6539
Fragments arranged by size:
6539 1555 32 32
PstI C_TGCA'G
Cuts at: 830 2037 2610 2730 3006 4302 4891 5123 5193
Size: 1207 573 120 276 1296 589 232 70
Cuts at: 5193 5396 5510 830
Size: 203 114 3478
Fragments arranged by size:
3478 1296 1207 589 573 276 232 203
120 114 70
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8158
SacI G_AGCT'C
Cuts at: 2748 2748
Size: 8158
SalI G'TCGA_C
Cuts at: 1936 5500 1936
Size: 3564 4594
SbfI CC_TGCA'GG
Cuts at: 3006 4302 5123 5510 3006
Size: 1296 821 387 5654
Fragments arranged by size:
5654 1296 821 387
ScaI AGT'ACT
Cuts at: 5099 6406 8118 5099
Size: 1307 1712 5139
Fragments arranged by size:
5139 1712 1307
SexAI A'CCwGG_T
Cuts at: 4392 4392
Size: 8158
SfcI C'TryA_G
Cuts at: 826 1045 2033 2606 2726 3002 3847 4298 4887
Size: 219 988 573 120 276 845 451 589
Cuts at: 4887 5119 5189 5392 5506 6641 7322 7513 826
Size: 232 70 203 114 1135 681 191 1471
Fragments arranged by size:
1471 1135 988 845 681 589 573 451
276 232 219 203 191 120 114 70
SfoI GGC'GCC
Cuts at: 2261 4171 2261
Size: 1910 6248
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8158
SgrAI Cr'CCGG_yG
Cuts at: 1700 5316 1700
Size: 3616 4542
SmaI CCC'GGG
Cuts at: 1872 2256 2283 2349 2919 5485 1872
Size: 384 27 66 570 2566 4545
Fragments arranged by size:
4545 2566 570 384 66 27
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2874 3272 4148 6262 7163 7425
Size: 197 926 931 398 876 2114 901 262
Cuts at: 7425 820
Size: 1553
Fragments arranged by size:
2114 1553 931 926 901 876 398 262
197
SnaBI TAC'GTA
Cuts at: 493 5949 493
Size: 5456 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8158
SphI G_CATG'C
Cuts at: 1132 3235 3835 5516 1132
Size: 2103 600 1681 3774
Fragments arranged by size:
3774 2103 1681 600
SspI AAT'ATT
Cuts at: 5 52 6082 5
Size: 47 6030 2081
Fragments arranged by size:
6030 2081 47
StuI AGG'CCT
Cuts at: 1517 2441 2997 4277 4478 5125 1517
Size: 924 556 1280 201 647 4550
Fragments arranged by size:
4550 1280 924 647 556 201
StyI C'CwwG_G
Cuts at: 513 1065 2004 2263 2382 2658 2966 3777 4236
Size: 552 939 259 119 276 308 811 459
Cuts at: 4236 4487 4623 4936 4945 5597 513
Size: 251 136 313 9 652 3074
Fragments arranged by size:
3074 939 811 652 552 459 313 308
276 259 251 136 119 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2711 4687 5303 5315 6185 6202 6355
Size: 1255 1131 1976 616 12 870 17 153
Cuts at: 6355 6540 6985 325
Size: 185 445 1498
Fragments arranged by size:
1976 1498 1255 1131 870 616 445 185
153 17 12
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2109 2619 3222
Size: 274 80 33 51 1443 132 510 603
Cuts at: 3222 5097 6404 8116 96
Size: 1875 1307 1712 138
Fragments arranged by size:
1875 1712 1443 1307 603 510 274 138
132 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2768 2800 5265 5297 8118 8150 2768
Size: 32 2465 32 2821 32 2776
Fragments arranged by size:
2821 2776 2465 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3662 3968 1316
Size: 2346 306 5506
Fragments arranged by size:
5506 2346 306
XbaI T'CTAG_A
Cuts at: 5494 5494
Size: 8158
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2816 2889 4315 5065 2011
Size: 805 73 1426 750 5104
Fragments arranged by size:
5104 1426 805 750 73
XhoI C'TCGA_G
Cuts at: 1943 2874 1943
Size: 931 7227
XmaI C'CCGG_G
Cuts at: 1870 2254 2281 2347 2917 5483 1870
Size: 384 27 66 570 2566 4545
Fragments arranged by size:
4545 2566 570 384 66 27
XmnI GAAnn'nnTTC
Cuts at: 3206 6287 3206
Size: 3081 5077
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5966 276
Size: 53 83 186 936 4432 2468
Fragments arranged by size:
4432 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI SacI SalI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FseI
FspAI NaeI NgoMIV NruI NsiI PacI PvuII RsrII
SacII SanDI SapI SfiI SrfI SwaI