Restriction map for
pFN21AA0950
(Circular) (Six-base) MAPSORT of: pFN21AA0950.seq Check: 9445 from: 1 to: 5653
pFN21AA0950 5653 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2053 2053
Size: 5653
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3463 278
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2322 2996 1937
Size: 385 674 4594
Fragments arranged by size:
4594 674 385
Acc65I G'GTAC_C
Cuts at: 2974 2974
Size: 5653
AclI AA'CG_TT
Cuts at: 1563 3780 4153 1563
Size: 2217 373 3063
Fragments arranged by size:
3063 2217 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2623 2695 3716 5100 5368 1632
Size: 991 72 1021 1384 268 1917
Fragments arranged by size:
1917 1384 1021 991 268 72
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5456
AflIII A'CryG_T
Cuts at: 1203 4552 5529 1203
Size: 3349 977 1327
Fragments arranged by size:
3349 1327 977
AgeI A'CCGG_T
Cuts at: 4484 4484
Size: 5653
AhdI GACnn_n'nnGTC
Cuts at: 4382 4382
Size: 5653
AleI CACnn'nnGTG
Cuts at: 1166 2177 2585 1166
Size: 1011 408 4234
Fragments arranged by size:
4234 1011 408
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5653
AlwNI CAG_nnn'CTG
Cuts at: 2347 2830 3396 4968 2347
Size: 483 566 1572 3032
Fragments arranged by size:
3032 1572 566 483
ApaLI G'TGCA_C
Cuts at: 3710 4866 3710
Size: 1156 4497
ApoI r'AATT_y
Cuts at: 1493 2962 3188 3340 5449 1493
Size: 1469 226 152 2109 1697
Fragments arranged by size:
2109 1697 1469 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4207 160
Size: 872 3175 1606
Fragments arranged by size:
3175 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
AvaI C'yCGr_G
Cuts at: 1870 1943 2978 1870
Size: 73 1035 4545
Fragments arranged by size:
4545 1035 73
BamHI G'GATC_C
Cuts at: 2983 3350 2983
Size: 367 5286
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2179 2974 4429 618
Size: 325 217 627 392 795 1455 1842
Fragments arranged by size:
1842 1455 795 627 392 325 217
BanII G_rGCy'C
Cuts at: 1391 2265 2861 2884 2972 1391
Size: 874 596 23 88 4072
Fragments arranged by size:
4072 874 596 88 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2629 928
Size: 971 730 3952
Fragments arranged by size:
3952 971 730
BbvCI CC'TCA_GC
Cuts at: 2101 2101
Size: 5653
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3844 3878 1903
Size: 34 1907 34 3678
Fragments arranged by size:
3678 1907 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3545 4755 3545
Size: 1210 4443
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5653
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4264 136
Size: 107 122 71 631 605 2592 1525
Fragments arranged by size:
2592 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5648 5648
Size: 5653
BlpI GC'TnA_GC
Cuts at: 2855 3070 2855
Size: 215 5438
Bme1580I G_kGCm'C
Cuts at: 1790 2100 2757 3714 4870 1790
Size: 310 657 957 1156 2573
Fragments arranged by size:
2573 1156 957 657 310
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5653
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2437 2562 2792
Size: 416 53 83 399 24 1020 125 230
Cuts at: 2792 2818 3384 4342 442
Size: 26 566 958 1753
Fragments arranged by size:
1753 1020 958 566 416 399 230 125
83 53 26 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2073 2209 4313 1239
Size: 834 136 2104 2579
Fragments arranged by size:
2579 2104 834 136
Bpu10I CC'TnA_GC
Cuts at: 2101 2101
Size: 5653
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3778 4643 4941 3778
Size: 865 298 4490
Fragments arranged by size:
4490 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2247 4316 882
Size: 617 748 2069 2219
Fragments arranged by size:
2219 2069 748 617
BsaAI yAC'GTr
Cuts at: 493 1204 3444 493
Size: 711 2240 2702
Fragments arranged by size:
2702 2240 711
BsaBI GATnn'nnATC
Cuts at: 5647 5647
Size: 5653
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2845 3460 3842 4477
Size: 53 83 186 936 1312 615 382 635
Cuts at: 4477 275
Size: 1451
Fragments arranged by size:
1451 1312 936 635 615 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2249 4086 4484 4758 4905 5316 2249
Size: 1837 398 274 147 411 2586
Fragments arranged by size:
2586 1837 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2567 2597 2651 2681 2778
Size: 30 165 30 1164 30 54 30 97
Cuts at: 2778 2808 1178
Size: 30 4023
Fragments arranged by size:
4023 1164 165 97 54 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2879 1186
Size: 1693 3960
BseYI C'CCAG_C
Cuts at: 2164 2196 2213 2280 4856 5352 2164
Size: 32 17 67 2576 496 2465
Fragments arranged by size:
2576 2465 496 67 32 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5080
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3103 3864 4892 1540
Size: 275 186 1102 761 1028 2301
Fragments arranged by size:
2301 1102 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2265 2685 3714 3799 4870 2265
Size: 420 1029 85 1071 3048
Fragments arranged by size:
3048 1071 1029 420 85
BsmI GAATG_Cn'
Cuts at: 3164 3257 3164
Size: 93 5560
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2265 2685 2757 2861 2884 2972
Size: 399 310 165 420 72 104 23 88
Cuts at: 2972 3714 3799 4870 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 420 399 310 165 104
88 85 72 23
BspEI T'CCGG_A
Cuts at: 5316 5316
Size: 5653
BspHI T'CATG_A
Cuts at: 2697 3542 5272 2697
Size: 845 1730 3078
Fragments arranged by size:
3078 1730 845
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2053 3008 844
Size: 1209 955 3489
Fragments arranged by size:
3489 1209 955
BsrBI CCG'CTC
Cuts at: 1394 1823 2789 3540 1394
Size: 429 966 751 3507
Fragments arranged by size:
3507 966 751 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4148 4322 65
Size: 1183 2900 174 1396
Fragments arranged by size:
2900 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4297 4484 1700
Size: 110 2487 187 2869
Fragments arranged by size:
2869 2487 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5653
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5653
BssSI C'ACGA_G
Cuts at: 3713 4725 3713
Size: 1012 4641
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2065 2438 2774 794
Size: 1271 373 336 3673
Fragments arranged by size:
3673 1271 373 336
BstBI TT'CG_AA
Cuts at: 4457 4457
Size: 5653
BstEII G'GTnAC_C
Cuts at: 1184 2350 2837 1184
Size: 1166 487 4000
Fragments arranged by size:
4000 1166 487
BstYI r'GATC_y
Cuts at: 1972 2983 3350 3735 3752 5204 5290 5302 5648
Size: 1011 367 385 17 1452 86 12 346
Cuts at: 5648 1972
Size: 1977
Fragments arranged by size:
1977 1452 1011 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2862 2862
Size: 5653
BtgI C'CryG_G
Cuts at: 513 1065 2025 2538 513
Size: 552 960 513 3628
Fragments arranged by size:
3628 960 552 513
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4914
Fragments arranged by size:
4914 683 56
BtsI GCAGTG_nn'
Cuts at: 2100 3169 3963 3983 2100
Size: 1069 794 20 3770
Fragments arranged by size:
3770 1069 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5653
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5618
DraI TTT'AAA
Cuts at: 2957 2957
Size: 5653
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4660 809
Size: 507 3344 1802
Fragments arranged by size:
3344 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2727 3100 3989
Size: 54 1425 50 275 18 897 373 889
Cuts at: 3989 5618 8
Size: 1629 43
Fragments arranged by size:
1629 1425 897 889 373 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3100 1537
Size: 275 1288 4090
Fragments arranged by size:
4090 1288 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2270 2690 3583 1353
Size: 917 420 893 3423
Fragments arranged by size:
3423 917 893 420
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4240 4614 4760 387
Size: 1064 138 2651 374 146 1280
Fragments arranged by size:
2651 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2263 2263
Size: 5653
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2073 2209 2623 2695 3716 4313 5100
Size: 393 441 136 414 72 1021 597 787
Cuts at: 5100 5368 1239
Size: 268 1524
Fragments arranged by size:
1524 1021 787 597 441 414 393 268
136 72
EcoO109I rG'GnC_Cy
Cuts at: 2038 2154 2038
Size: 116 5537
EcoRI G'AATT_C
Cuts at: 2962 3340 2962
Size: 378 5275
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2677 2677
Size: 5653
FspI TGC'GCA
Cuts at: 4159 4159
Size: 5653
HaeII r_GCGC'y
Cuts at: 1678 4800 1678
Size: 3122 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2295 2327 2651 2683 4294
Size: 32 135 32 723 32 324 32 1611
Cuts at: 4294 4326 4368 4400 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1611 723 324 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2997 3243 669
Size: 988 281 1059 246 3079
Fragments arranged by size:
3079 1059 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5653
HpaI GTT'AAC
Cuts at: 3243 3243
Size: 5653
KpnI G_GTAC'C
Cuts at: 2978 2978
Size: 5653
MfeI C'AATT_G
Cuts at: 3252 3252
Size: 5653
MluI A'CGCG_T
Cuts at: 5529 5529
Size: 5653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4767 4951 1305
Size: 3462 184 2007
Fragments arranged by size:
3462 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2729 10
Size: 54 1425 343 897 2934
Fragments arranged by size:
2934 1425 897 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 2555 2585 3611 3970 4129 518
Size: 648 1011 378 30 1026 359 159 2042
Fragments arranged by size:
2042 1026 1011 648 378 359 159 30
MspA1I CmG'CkG
Cuts at: 1606 2177 2280 2344 2715 3069 3746 4894 5139
Size: 571 103 64 371 354 677 1148 245
Cuts at: 5139 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 571 371 354 245 103
64
NcoI C'CATG_G
Cuts at: 513 1065 2538 513
Size: 552 1473 3628
Fragments arranged by size:
3628 1473 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5653
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5653
NotI GC'GGCC_GC
Cuts at: 3100 3100
Size: 5653
NspI r_CATG'y
Cuts at: 1132 1229 3011 4556 1132
Size: 97 1782 1545 2229
Fragments arranged by size:
2229 1782 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2011 2042 1348
Size: 663 31 4959
Fragments arranged by size:
4959 663 31
PciI A'CATG_T
Cuts at: 4552 4552
Size: 5653
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5653
PmeI GTTT'AAAC
Cuts at: 2957 2957
Size: 5653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3722 3754 5235 5267 3722
Size: 32 1481 32 4108
Fragments arranged by size:
4108 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5653
PsiI TTA'TAA
Cuts at: 3223 3223
Size: 5653
PstI C_TGCA'G
Cuts at: 830 2931 3005 830
Size: 2101 74 3478
Fragments arranged by size:
3478 2101 74
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5653
PvuII CAG'CTG
Cuts at: 2280 2344 2280
Size: 64 5589
SacI G_AGCT'C
Cuts at: 2265 2265
Size: 5653
SalI G'TCGA_C
Cuts at: 1936 2995 1936
Size: 1059 4594
SapI GCTCTTCn'nnn_
Cuts at: 2270 2690 2270
Size: 420 5233
SbfI CC_TGCA'GG
Cuts at: 3005 3005
Size: 5653
ScaI AGT'ACT
Cuts at: 3901 5613 3901
Size: 1712 3941
SfcI C'TryA_G
Cuts at: 826 1045 2927 3001 4136 4817 5008 826
Size: 219 1882 74 1135 681 191 1471
Fragments arranged by size:
1882 1471 1135 681 219 191 74
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5653
SmaI CCC'GGG
Cuts at: 1872 2980 1872
Size: 1108 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3757 4658 4920 820
Size: 197 926 1814 901 262 1553
Fragments arranged by size:
1814 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3444 493
Size: 2951 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5653
SphI G_CATG'C
Cuts at: 1132 3011 1132
Size: 1879 3774
SspI AAT'ATT
Cuts at: 5 52 3577 5
Size: 47 3525 2081
Fragments arranged by size:
3525 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5653
StyI C'CwwG_G
Cuts at: 513 1065 2355 2538 3092 513
Size: 552 1290 183 554 3074
Fragments arranged by size:
3074 1290 554 552 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2063 2837 3680 3697 3850 4035 4480
Size: 1255 483 774 843 17 153 185 445
Cuts at: 4480 325
Size: 1498
Fragments arranged by size:
1498 1255 843 774 483 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3899 5611 96
Size: 274 80 33 51 1443 1922 1712 138
Fragments arranged by size:
1922 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5613 5645 5613
Size: 32 5621
Tth111I GACn'n_nGTC
Cuts at: 1316 2390 2402 1316
Size: 1074 12 4567
Fragments arranged by size:
4567 1074 12
XbaI T'CTAG_A
Cuts at: 2989 2989
Size: 5653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2424 2424
Size: 5653
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5653
XmaI C'CCGG_G
Cuts at: 1870 2978 1870
Size: 1108 4545
XmnI GAAnn'nnTTC
Cuts at: 3782 3782
Size: 5653
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3461 276
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI