Restriction map for
pFN21AA0949
(Circular) (Six-base) MAPSORT of: pFN21AA0949.seq Check: 8701 from: 1 to: 9340
pFN21AA0949 9340 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4799 5171 5240 5580 5890 5920 4799
Size: 372 69 340 310 30 8219
Fragments arranged by size:
8219 372 340 310 69 30
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7150 278
Size: 53 83 186 936 5614 2468
Fragments arranged by size:
5614 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6683 1937
Size: 4746 4594
Acc65I G'GTAC_C
Cuts at: 6661 6661
Size: 9340
AclI AA'CG_TT
Cuts at: 1563 7467 7840 1563
Size: 5904 373 3063
Fragments arranged by size:
5904 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2105 2415 2484 2676 2982 3381 3671 3962
Size: 473 310 69 192 306 399 290 291
Cuts at: 3962 4187 4364 4382 5156 5344 6618 7403 8787
Size: 225 177 18 774 188 1274 785 1384
Cuts at: 8787 9055 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1274 785 774 473 399 310
306 291 290 268 225 192 188 177
69 18
AfeI AGC'GCT
Cuts at: 5219 5219
Size: 9340
AflII C'TTAA_G
Cuts at: 820 1017 4619 820
Size: 197 3602 5541
Fragments arranged by size:
5541 3602 197
AflIII A'CryG_T
Cuts at: 1203 8239 9216 1203
Size: 7036 977 1327
Fragments arranged by size:
7036 1327 977
AgeI A'CCGG_T
Cuts at: 8171 8171
Size: 9340
AhdI GACnn_n'nnGTC
Cuts at: 2421 8069 2421
Size: 5648 3692
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9340
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2142 4907 1184
Size: 958 2765 5617
Fragments arranged by size:
5617 2765 958
AlwNI CAG_nnn'CTG
Cuts at: 3214 3359 3940 4030 5282 5557 7083 8655 3214
Size: 145 581 90 1252 275 1526 1572 3899
Fragments arranged by size:
3899 1572 1526 1252 581 275 145 90
ApaI G_GGCC'C
Cuts at: 6188 6302 6188
Size: 114 9226
ApaLI G'TGCA_C
Cuts at: 2741 3661 4709 7397 8553 2741
Size: 920 1048 2688 1156 3528
Fragments arranged by size:
3528 2688 1156 1048 920
ApoI r'AATT_y
Cuts at: 1493 2399 2517 2880 3053 3410 4604 5036 5463
Size: 906 118 363 173 357 1194 432 427
Cuts at: 5463 5810 5849 5978 6649 6875 7027 9136 1493
Size: 347 39 129 671 226 152 2109 1697
Fragments arranged by size:
2109 1697 1194 906 671 432 427 363
357 347 226 173 152 129 118 39
AseI AT'TA_AT
Cuts at: 160 1032 7894 160
Size: 872 6862 1606
Fragments arranged by size:
6862 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9340
AvaI C'yCGr_G
Cuts at: 1870 1943 4428 5171 5843 6147 6539 6665 1870
Size: 73 2485 743 672 304 392 126 4545
Fragments arranged by size:
4545 2485 743 672 392 304 126 73
BamHI G'GATC_C
Cuts at: 6670 7037 6670
Size: 367 8973
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3542 4915 5078 5384 5878
Size: 325 217 627 1755 1373 163 306 494
Cuts at: 5878 6661 8116 618
Size: 783 1455 1842
Fragments arranged by size:
1842 1755 1455 1373 783 627 494 325
306 217 163
BanII G_rGCy'C
Cuts at: 1391 2529 2694 3222 4050 4419 4881 5400 5661
Size: 1138 165 528 828 369 462 519 261
Cuts at: 5661 5760 6188 6302 6659 1391
Size: 99 428 114 357 4072
Fragments arranged by size:
4072 1138 828 528 519 462 428 369
357 261 165 114 99
BbeI G_GCGC'C
Cuts at: 3546 3546
Size: 9340
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2350 3335 3659 4175 4409 4581 5449
Size: 971 451 985 324 516 234 172 868
Cuts at: 5449 6013 6561 6624 928
Size: 564 548 63 3644
Fragments arranged by size:
3644 985 971 868 564 548 516 451
324 234 172 63
BbvCI CC'TCA_GC
Cuts at: 2685 5717 6130 2685
Size: 3032 413 5895
Fragments arranged by size:
5895 3032 413
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7531 7565 1903
Size: 34 5594 34 3678
Fragments arranged by size:
5594 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4696 5780 5801 7232 8442 4696
Size: 1084 21 1431 1210 5594
Fragments arranged by size:
5594 1431 1210 1084 21
BclI T'GATC_A
Cuts at: 1548 2904 1548
Size: 1356 7984
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3287 7951 136
Size: 107 122 71 631 605 1615 4664 1525
Fragments arranged by size:
4664 1615 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 9335 9335
Size: 9340
BlpI GC'TnA_GC
Cuts at: 2502 6515 6757 2502
Size: 4013 242 5085
Fragments arranged by size:
5085 4013 242
Bme1580I G_kGCm'C
Cuts at: 1790 2745 3665 4713 4920 5387 5521 6188 6302
Size: 955 920 1048 207 467 134 667 114
Cuts at: 6302 7401 8557 1790
Size: 1099 1156 2573
Fragments arranged by size:
2573 1156 1099 1048 955 920 667 467
207 134 114
BmgBI CAC'GTC
Cuts at: 1318 5536 1318
Size: 4218 5122
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3766 4536 5557
Size: 416 53 83 399 24 2349 770 1021
Cuts at: 5557 7071 8029 442
Size: 1514 958 1753
Fragments arranged by size:
2349 1753 1514 1021 958 770 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 5195 1056
Size: 4139 5201
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2397 3973 2397
Size: 1576 7764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2135 2507 2561 2645 2852 3083 3125 3137
Size: 896 372 54 84 207 231 42 12
Cuts at: 3137 3188 3236 3326 3473 3692 3763 3998 4052
Size: 51 48 90 147 219 71 235 54
Cuts at: 4052 4376 4581 4850 4991 5504 6358 6506 8000
Size: 324 205 269 141 513 854 148 1494
Cuts at: 8000 1239
Size: 2579
Fragments arranged by size:
2579 1494 896 854 513 372 324 269
235 231 219 207 205 148 147 141
90 84 71 54 54 51 48 42
12
Bpu10I CC'TnA_GC
Cuts at: 2445 2685 5717 6130 2445
Size: 240 3032 413 5655
Fragments arranged by size:
5655 3032 413 240
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2268 3206 4113 6014 7465 8330 8628 2268
Size: 938 907 1901 1451 865 298 2980
Fragments arranged by size:
2980 1901 1451 938 907 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3338 4868 5741 8003 882
Size: 617 1839 1530 873 2262 2219
Fragments arranged by size:
2262 2219 1839 1530 873 617
BsaAI yAC'GTr
Cuts at: 493 1204 3660 4776 6342 7131 493
Size: 711 2456 1116 1566 789 2702
Fragments arranged by size:
2702 2456 1566 1116 789 711
BsaBI GATnn'nnATC
Cuts at: 9334 9334
Size: 9340
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3543 4615 4720 6218
Size: 53 83 186 936 2010 1072 105 1498
Cuts at: 6218 7147 7529 8164 275
Size: 929 382 635 1451
Fragments arranged by size:
2010 1498 1451 1072 936 929 635 382
186 105 83 53
BsaWI w'CCGG_w
Cuts at: 4419 5733 7773 8171 8445 8592 9003 4419
Size: 1314 2040 398 274 147 411 4756
Fragments arranged by size:
4756 2040 1314 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3251 3281 5976 6006 1178
Size: 30 165 30 1848 30 2695 30 4512
Fragments arranged by size:
4512 2695 1848 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2702 2870 2873 3122 3218 3380 3470 3760
Size: 1516 168 3 249 96 162 90 290
Cuts at: 3760 5969 6118 1186
Size: 2209 149 4408
Fragments arranged by size:
4408 2209 1516 290 249 168 162 149
96 90 3
BseYI C'CCAG_C
Cuts at: 2727 3074 4880 4947 5198 5220 5580 5681 6379
Size: 347 1806 67 251 22 360 101 698
Cuts at: 6379 6398 8543 9039 2727
Size: 19 2145 496 3028
Fragments arranged by size:
3028 2145 1806 698 496 360 347 251
101 67 22 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3276 4398 5332 5691 5948 1163
Size: 573 1540 1122 934 359 257 4555
Fragments arranged by size:
4555 1540 1122 934 573 359 257
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4526 6508 6790 7551 8579 1540
Size: 275 186 2525 1982 282 761 1028 2301
Fragments arranged by size:
2525 2301 1982 1028 761 282 275 186
BsiHKAI G_wGCw'C
Cuts at: 2529 2694 2745 3201 3222 3665 3894 4050 4419
Size: 165 51 456 21 443 229 156 369
Cuts at: 4419 4536 4713 4772 6354 7401 7486 8557 2529
Size: 117 177 59 1582 1047 85 1071 3312
Fragments arranged by size:
3312 1582 1071 1047 456 443 369 229
177 165 156 117 85 59 51 21
BsmI GAATG_Cn'
Cuts at: 2150 3810 4012 6851 6944 2150
Size: 1660 202 2839 93 4546
Fragments arranged by size:
4546 2839 1660 202 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2529 2694 2745 3201 3222 3665 3894
Size: 399 739 165 51 456 21 443 229
Cuts at: 3894 4050 4419 4536 4713 4772 4881 4920 5387
Size: 156 369 117 177 59 109 39 467
Cuts at: 5387 5400 5521 5661 5760 6188 6302 6354 6659
Size: 13 121 140 99 428 114 52 305
Cuts at: 6659 7401 7486 8557 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 739 467 456 443 428
399 369 305 229 177 165 156 140
121 117 114 109 99 85 59 52
51 39 21 13
BspEI T'CCGG_A
Cuts at: 5733 9003 5733
Size: 3270 6070
BspHI T'CATG_A
Cuts at: 4225 5496 7229 8959 4225
Size: 1271 1733 1730 4606
Fragments arranged by size:
4606 1733 1730 1271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4107 4312 4799 5171 5240 5580 5890 5920
Size: 3263 205 487 372 69 340 310 30
Cuts at: 5920 6695 844
Size: 775 3489
Fragments arranged by size:
3489 3263 775 487 372 340 310 205
69 30
BsrBI CCG'CTC
Cuts at: 1394 1823 3727 5515 6532 7227 1394
Size: 429 1904 1788 1017 695 3507
Fragments arranged by size:
3507 1904 1788 1017 695 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 7835 8009 65
Size: 1183 6587 174 1396
Fragments arranged by size:
6587 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5026 6570 7984 8171 1700
Size: 110 3216 1544 1414 187 2869
Fragments arranged by size:
3216 2869 1544 1414 187 110
BsrGI T'GTAC_A
Cuts at: 96 3559 96
Size: 3463 5877
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9340
BssSI C'ACGA_G
Cuts at: 2852 3200 6266 7400 8412 2852
Size: 348 3066 1134 1012 3780
Fragments arranged by size:
3780 3066 1134 1012 348
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3805 3994 5727 794
Size: 3011 189 1733 4407
Fragments arranged by size:
4407 3011 1733 189
BstBI TT'CG_AA
Cuts at: 8144 8144
Size: 9340
BstEII G'GTnAC_C
Cuts at: 1184 5224 1184
Size: 4040 5300
BstXI CCAn_nnnn'nTGG
Cuts at: 5633 5981 6367 5633
Size: 348 386 8606
Fragments arranged by size:
8606 386 348
BstYI r'GATC_y
Cuts at: 1972 2105 2326 2930 3369 6114 6670 7037 7422
Size: 133 221 604 439 2745 556 367 385
Cuts at: 7422 7439 8891 8977 8989 9335 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2745 1977 1452 604 556 439 385 367
346 221 133 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 6199 6199
Size: 9340
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 8788
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2915 487
Size: 683 56 1689 6912
Fragments arranged by size:
6912 1689 683 56
BtsI GCAGTG_nn'
Cuts at: 2210 3737 3888 6856 7650 7670 2210
Size: 1527 151 2968 794 20 3880
Fragments arranged by size:
3880 2968 1527 794 151 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9340
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9305
DraI TTT'AAA
Cuts at: 6644 6644
Size: 9340
DraIII CAC_nnn'GTG
Cuts at: 4686 4686
Size: 9340
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6601 8347 809
Size: 507 5285 1746 1802
Fragments arranged by size:
5285 1802 1746 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 6475 6505 6787
Size: 54 1425 50 275 18 4645 30 282
Cuts at: 6787 7676 9305 8
Size: 889 1629 43
Fragments arranged by size:
4645 1629 1425 889 282 275 54 50
43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 6505 6787 1537
Size: 275 4693 282 4090
Fragments arranged by size:
4693 4090 282 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2316 2691 3018 3336 3357 3426 4062 5505
Size: 963 375 327 318 21 69 636 1443
Cuts at: 5505 5905 7270 1353
Size: 400 1365 3423
Fragments arranged by size:
3423 1443 1365 963 636 400 375 327
318 69 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2582 3578 4414 4501 6460 7927
Size: 1064 138 993 996 836 87 1959 1467
Cuts at: 7927 8301 8447 387
Size: 374 146 1280
Fragments arranged by size:
1959 1467 1280 1064 996 993 836 374
146 138 87
EcoICRI GAG'CTC
Cuts at: 2527 2692 3220 4048 4417 2527
Size: 165 528 828 369 7450
Fragments arranged by size:
7450 828 528 369 165
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2105 2135 2415 2484 2507 2561 2645
Size: 393 473 30 280 69 23 54 84
Cuts at: 2645 2676 2852 2982 3083 3125 3137 3188 3236
Size: 31 176 130 101 42 12 51 48
Cuts at: 3236 3326 3381 3473 3671 3692 3763 3962 3998
Size: 90 55 92 198 21 71 199 36
Cuts at: 3998 4052 4187 4364 4376 4382 4581 4850 4991
Size: 54 135 177 12 6 199 269 141
Cuts at: 4991 5156 5344 5504 6358 6506 6618 7403 8000
Size: 165 188 160 854 148 112 785 597
Cuts at: 8000 8787 9055 1239
Size: 787 268 1524
Fragments arranged by size:
1524 854 787 785 597 473 393 280
269 268 199 199 198 188 177 176
165 160 148 141 135 130 112 101
92 90 84 71 69 55 54 54
51 48 42 36 31 30 23 21
12 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2117 4358 6269 2117
Size: 2241 1911 5188
Fragments arranged by size:
5188 2241 1911
EcoO109I rG'GnC_Cy
Cuts at: 2122 3697 5479 6138 6185 6298 6395 6594 2122
Size: 1575 1782 659 47 113 97 199 4868
Fragments arranged by size:
4868 1782 1575 659 199 113 97 47
EcoRI G'AATT_C
Cuts at: 2399 5849 6649 7027 2399
Size: 3450 800 378 4712
Fragments arranged by size:
4712 3450 800 378
EcoRV GAT'ATC
Cuts at: 2230 2230
Size: 9340
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2345 2345
Size: 9340
FspI TGC'GCA
Cuts at: 6458 7846 6458
Size: 1388 7952
HaeII r_GCGC'y
Cuts at: 1678 3229 3546 5221 6386 8487 1678
Size: 1551 317 1675 1165 2101 2531
Fragments arranged by size:
2531 2101 1675 1551 1165 317
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2296 2318 2328 2350 2407
Size: 32 135 32 724 22 10 22 57
Cuts at: 2407 2439 3251 3283 3384 3416 3631 3663 3752
Size: 32 812 32 101 32 215 32 89
Cuts at: 3752 3784 3826 3858 3895 3927 4406 4438 4968
Size: 32 42 32 37 32 479 32 530
Cuts at: 4968 5000 5131 5163 7981 8013 8055 8087 1373
Size: 32 131 32 2818 32 42 32 2626
Fragments arranged by size:
2818 2626 812 724 530 479 215 135
131 101 89 57 42 42 37 32
32 32 32 32 32 32 32 32
32 32 32 32 32 22 22 10
HincII GTy'rAC
Cuts at: 669 1657 1938 3276 6684 6930 669
Size: 988 281 1338 3408 246 3079
Fragments arranged by size:
3408 3079 1338 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4015 748
Size: 3267 6073
HpaI GTT'AAC
Cuts at: 6930 6930
Size: 9340
KasI G'GCGC_C
Cuts at: 3542 3542
Size: 9340
KpnI G_GTAC'C
Cuts at: 6665 6665
Size: 9340
MfeI C'AATT_G
Cuts at: 6939 6939
Size: 9340
MluI A'CGCG_T
Cuts at: 9216 9216
Size: 9340
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2298 2769 3063 5928 8454 8638 1305
Size: 993 471 294 2865 2526 184 2007
Fragments arranged by size:
2865 2526 2007 993 471 294 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 7518
Fragments arranged by size:
7518 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3393 4732 7298 7657 7816 518
Size: 648 2227 1339 2566 359 159 2042
Fragments arranged by size:
2566 2227 2042 1339 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3115 3432 3472 3847 4198 5766 6601 6756
Size: 1509 317 40 375 351 1568 835 155
Cuts at: 6756 7433 8581 8826 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1568 1509 1148 835 677 375 351
317 245 155 40
NaeI GCC'GGC
Cuts at: 6572 6572
Size: 9340
NarI GG'CG_CC
Cuts at: 3543 3543
Size: 9340
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 8788
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9340
NgoMIV G'CCGG_C
Cuts at: 6570 6570
Size: 9340
NheI G'CTAG_C
Cuts at: 1052 5191 1052
Size: 4139 5201
NotI GC'GGCC_GC
Cuts at: 6787 6787
Size: 9340
NspI r_CATG'y
Cuts at: 1132 1229 3992 4633 4675 6698 8243 1132
Size: 97 2763 641 42 2023 1545 2229
Fragments arranged by size:
2763 2229 2023 1545 641 97 42
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9340
PciI A'CATG_T
Cuts at: 8239 8239
Size: 9340
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3871 5166 5447 5875 1108
Size: 2763 1295 281 428 4573
Fragments arranged by size:
4573 2763 1295 428 281
PfoI T'CCnGG_A
Cuts at: 2112 4968 5853 2112
Size: 2856 885 5599
Fragments arranged by size:
5599 2856 885
PmeI GTTT'AAAC
Cuts at: 6644 6644
Size: 9340
PmlI CAC'GTG
Cuts at: 3660 4776 3660
Size: 1116 8224
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4032 4064 7409 7441 8922 8954 4032
Size: 32 3345 32 1481 32 4418
Fragments arranged by size:
4418 3345 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2122 5479 6138 6594 2122
Size: 3357 659 456 4868
Fragments arranged by size:
4868 3357 659 456
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9340
PsiI TTA'TAA
Cuts at: 6910 6910
Size: 9340
PspOMI G'GGCC_C
Cuts at: 6184 6298 6184
Size: 114 9226
PspXI vC'TCGA_Gb
Cuts at: 5843 5843
Size: 9340
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 6161 6193 6161
Size: 32 9308
PstI C_TGCA'G
Cuts at: 830 2217 2586 2637 3258 3288 3801 3918 4104
Size: 1387 369 51 621 30 513 117 186
Cuts at: 4104 4332 5765 6692 830
Size: 228 1433 927 3478
Fragments arranged by size:
3478 1433 1387 927 621 513 369 228
186 117 51 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9340
PvuII CAG'CTG
Cuts at: 3115 3432 3472 4198 5766 3115
Size: 317 40 726 1568 6689
Fragments arranged by size:
6689 1568 726 317 40
RsrII CG'GwC_CG
Cuts at: 4422 6447 4422
Size: 2025 7315
SacI G_AGCT'C
Cuts at: 2529 2694 3222 4050 4419 2529
Size: 165 528 828 369 7450
Fragments arranged by size:
7450 828 528 369 165
SalI G'TCGA_C
Cuts at: 1936 6682 1936
Size: 4746 4594
SanDI GG'GwC_CC
Cuts at: 6138 6138
Size: 9340
SapI GCTCTTCn'nnn_
Cuts at: 3426 4062 5505 3426
Size: 636 1443 7261
Fragments arranged by size:
7261 1443 636
SbfI CC_TGCA'GG
Cuts at: 3258 3288 6692 3258
Size: 30 3404 5906
Fragments arranged by size:
5906 3404 30
ScaI AGT'ACT
Cuts at: 5960 7588 9300 5960
Size: 1628 1712 6000
Fragments arranged by size:
6000 1712 1628
SexAI A'CCwGG_T
Cuts at: 5367 5367
Size: 9340
SfcI C'TryA_G
Cuts at: 826 1045 2213 2582 2633 3242 3254 3284 3797
Size: 219 1168 369 51 609 12 30 513
Cuts at: 3797 3914 4100 4328 5761 6061 6688 7823 8504
Size: 117 186 228 1433 300 627 1135 681
Cuts at: 8504 8695 826
Size: 191 1471
Fragments arranged by size:
1471 1433 1168 1135 681 627 609 513
369 300 228 219 191 186 117 51
30 12
SfoI GGC'GCC
Cuts at: 3544 3544
Size: 9340
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9340
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9340
SmaI CCC'GGG
Cuts at: 1872 4430 5173 6541 6667 1872
Size: 2558 743 1368 126 4545
Fragments arranged by size:
4545 2558 1368 743 126
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2283 3221 4128 4619 5843 6029
Size: 197 926 340 938 907 491 1224 186
Cuts at: 6029 7444 8345 8607 820
Size: 1415 901 262 1553
Fragments arranged by size:
1553 1415 1224 938 926 907 901 491
340 262 197 186
SnaBI TAC'GTA
Cuts at: 493 7131 493
Size: 6638 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9340
SphI G_CATG'C
Cuts at: 1132 3992 4633 6698 1132
Size: 2860 641 2065 3774
Fragments arranged by size:
3774 2860 2065 641
SspI AAT'ATT
Cuts at: 5 52 4642 7264 5
Size: 47 4590 2622 2081
Fragments arranged by size:
4590 2622 2081 47
StuI AGG'CCT
Cuts at: 1517 2090 3740 4832 1517
Size: 573 1650 1092 6025
Fragments arranged by size:
6025 1650 1092 573
StyI C'CwwG_G
Cuts at: 513 1065 4872 5874 6779 513
Size: 552 3807 1002 905 3074
Fragments arranged by size:
3807 3074 1002 905 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3491 3746 4479 4485 5611 6352 6464
Size: 1255 1911 255 733 6 1126 741 112
Cuts at: 6464 7367 7384 7537 7722 8167 325
Size: 903 17 153 185 445 1498
Fragments arranged by size:
1911 1498 1255 1126 903 741 733 445
255 185 153 112 17 6
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3434 3559 4332
Size: 274 80 33 51 1443 1457 125 773
Cuts at: 4332 4959 5835 5958 7586 9298 96
Size: 627 876 123 1628 1712 138
Fragments arranged by size:
1712 1628 1457 1443 876 773 627 274
138 125 123 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5976 6008 6252 6284 9300 9332 5976
Size: 32 244 32 3016 32 5984
Fragments arranged by size:
5984 3016 244 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9340
XbaI T'CTAG_A
Cuts at: 3193 4147 5335 6676 3193
Size: 954 1188 1341 5857
Fragments arranged by size:
5857 1341 1188 954
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4555 4555
Size: 9340
XhoI C'TCGA_G
Cuts at: 1943 5843 1943
Size: 3900 5440
XmaI C'CCGG_G
Cuts at: 1870 4428 5171 6539 6665 1870
Size: 2558 743 1368 126 4545
Fragments arranged by size:
4545 2558 1368 743 126
XmnI GAAnn'nnTTC
Cuts at: 4206 4395 7469 4206
Size: 189 3074 6077
Fragments arranged by size:
6077 3074 189
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7148 276
Size: 53 83 186 936 5614 2468
Fragments arranged by size:
5614 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I
FseI FspAI NruI NsiI PacI SacII SfiI SrfI
SwaI