Restriction map for
pFN21AA0946
(Circular) (Six-base) MAPSORT of: pFN21AA0946.seq Check: 8969 from: 1 to: 6601
pFN21AA0946 6601 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4411 278
Size: 53 83 186 936 2875 2468
Fragments arranged by size:
2875 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3854 3944 1937
Size: 1917 90 4594
Fragments arranged by size:
4594 1917 90
Acc65I G'GTAC_C
Cuts at: 3888 3922 3888
Size: 34 6567
AclI AA'CG_TT
Cuts at: 1563 4728 5101 1563
Size: 3165 373 3063
Fragments arranged by size:
3165 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4664 6048 6316 1632
Size: 3032 1384 268 1917
Fragments arranged by size:
3032 1917 1384 268
AfeI AGC'GCT
Cuts at: 2787 2787
Size: 6601
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6404
AflIII A'CryG_T
Cuts at: 1203 3581 5500 6477 1203
Size: 2378 1919 977 1327
Fragments arranged by size:
2378 1919 1327 977
AgeI A'CCGG_T
Cuts at: 3343 5432 3343
Size: 2089 4512
AhdI GACnn_n'nnGTC
Cuts at: 2869 3297 5330 2869
Size: 428 2033 4140
Fragments arranged by size:
4140 2033 428
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6601
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6601
AlwNI CAG_nnn'CTG
Cuts at: 3327 3884 4344 5916 3327
Size: 557 460 1572 4012
Fragments arranged by size:
4012 1572 557 460
ApaLI G'TGCA_C
Cuts at: 4658 5814 4658
Size: 1156 5445
ApoI r'AATT_y
Cuts at: 1493 2374 2903 3104 3389 3800 3910 4136 4288
Size: 881 529 201 285 411 110 226 152
Cuts at: 4288 6397 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 881 529 411 285 226 201
152 110
AseI AT'TA_AT
Cuts at: 160 1032 2459 5155 160
Size: 872 1427 2696 1606
Fragments arranged by size:
2696 1606 1427 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6601
AvaI C'yCGr_G
Cuts at: 1870 1943 3926 1870
Size: 73 1983 4545
Fragments arranged by size:
4545 1983 73
BamHI G'GATC_C
Cuts at: 2617 3931 4298 2617
Size: 1314 367 4920
Fragments arranged by size:
4920 1314 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3888 3922 5377 618
Size: 325 217 627 2101 34 1455 1842
Fragments arranged by size:
2101 1842 1455 627 325 217 34
BanII G_rGCy'C
Cuts at: 1391 3920 1391
Size: 2529 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5630
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4792 4826 1903
Size: 34 50 34 2771 34 3678
Fragments arranged by size:
3678 2771 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4493 5703 4493
Size: 1210 5391
BclI T'GATC_A
Cuts at: 1548 2823 3394 1548
Size: 1275 571 4755
Fragments arranged by size:
4755 1275 571
BfrBI ATG'CAT
Cuts at: 3067 3067
Size: 6601
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5212 136
Size: 107 122 71 631 605 3540 1525
Fragments arranged by size:
3540 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6596 6596
Size: 6601
BlpI GC'TnA_GC
Cuts at: 4018 4018
Size: 6601
Bme1580I G_kGCm'C
Cuts at: 1790 2082 4662 5818 1790
Size: 292 2580 1156 2573
Fragments arranged by size:
2580 2573 1156 292
BmgBI CAC'GTC
Cuts at: 1318 3123 1318
Size: 1805 4796
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2401 3523 4332
Size: 416 53 83 399 24 984 1122 809
Cuts at: 4332 5290 442
Size: 958 1753
Fragments arranged by size:
1753 1122 984 958 809 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2708 1056
Size: 1652 4949
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2766 5261 1239
Size: 1527 2495 2579
Fragments arranged by size:
2579 2495 1527
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4726 5591 5889 4726
Size: 865 298 5438
Fragments arranged by size:
5438 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3524 5264 882
Size: 617 2025 1740 2219
Fragments arranged by size:
2219 2025 1740 617
BsaAI yAC'GTr
Cuts at: 493 1204 3582 4392 493
Size: 711 2378 810 2702
Fragments arranged by size:
2702 2378 810 711
BsaBI GATnn'nnATC
Cuts at: 6595 6595
Size: 6601
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2579 4408 4790 5425
Size: 53 83 186 936 1046 1829 382 635
Cuts at: 5425 275
Size: 1451
Fragments arranged by size:
1829 1451 1046 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2260 3343 5034 5432 5706 5853 6264 2260
Size: 1083 1691 398 274 147 411 2597
Fragments arranged by size:
2597 1691 1083 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2739 2769 1178
Size: 30 165 30 1336 30 5010
Fragments arranged by size:
5010 1336 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 2273 1186
Size: 844 243 5514
Fragments arranged by size:
5514 844 243
BseYI C'CCAG_C
Cuts at: 5804 6300 5804
Size: 496 6105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2466 3450 1163
Size: 573 730 984 4314
Fragments arranged by size:
4314 984 730 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4051 4812 5840 1540
Size: 275 186 2050 761 1028 2301
Fragments arranged by size:
2301 2050 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4662 4747 5818 4662
Size: 85 1071 5445
Fragments arranged by size:
5445 1071 85
BsmI GAATG_Cn'
Cuts at: 3712 4112 4205 3712
Size: 400 93 6108
Fragments arranged by size:
6108 400 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2082 3920 4662 4747 5818 1391
Size: 399 292 1838 742 85 1071 2174
Fragments arranged by size:
2174 1838 1071 742 399 292 85
BspEI T'CCGG_A
Cuts at: 6264 6264
Size: 6601
BspHI T'CATG_A
Cuts at: 4490 6220 4490
Size: 1730 4871
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2231 3956 844
Size: 1387 1725 3489
Fragments arranged by size:
3489 1725 1387
BsrBI CCG'CTC
Cuts at: 1394 1823 4488 1394
Size: 429 2665 3507
Fragments arranged by size:
3507 2665 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2384 3074 3400 5096 5270 65
Size: 1183 1136 690 326 1696 174 1396
Fragments arranged by size:
1696 1396 1183 1136 690 326 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3343 5245 5432 1700
Size: 110 1533 1902 187 2869
Fragments arranged by size:
2869 1902 1533 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6601
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6601
BssSI C'ACGA_G
Cuts at: 4661 5673 4661
Size: 1012 5589
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6601
BstBI TT'CG_AA
Cuts at: 2336 5405 2336
Size: 3069 3532
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6601
BstXI CCAn_nnnn'nTGG
Cuts at: 2132 2391 2132
Size: 259 6342
BstYI r'GATC_y
Cuts at: 1972 2449 2617 3229 3881 3931 4298 4683 4700
Size: 477 168 612 652 50 367 385 17
Cuts at: 4700 6152 6238 6250 6596 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 652 612 477 385 367 346
168 86 50 17 12
BstZ17I GTA'TAC
Cuts at: 3855 3855
Size: 6601
BtgI C'CryG_G
Cuts at: 513 1065 2125 2418 3407 3766 513
Size: 552 1060 293 989 359 3348
Fragments arranged by size:
3348 1060 989 552 359 293
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5862
Fragments arranged by size:
5862 683 56
BtsI GCAGTG_nn'
Cuts at: 2682 2706 4117 4911 4931 2682
Size: 24 1411 794 20 4352
Fragments arranged by size:
4352 1411 794 24 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6601
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3343 3378 529
Size: 35 2779 35 3752
Fragments arranged by size:
3752 2779 35 35
DraI TTT'AAA
Cuts at: 3730 3905 3730
Size: 175 6426
DraIII CAC_nnn'GTG
Cuts at: 2612 2612
Size: 6601
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5608 809
Size: 507 4292 1802
Fragments arranged by size:
4292 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4048 4937 6566
Size: 54 1425 50 275 18 2218 889 1629
Cuts at: 6566 8
Size: 43
Fragments arranged by size:
2218 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4048 1537
Size: 275 2236 4090
Fragments arranged by size:
4090 2236 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3249 4531 1353
Size: 1896 1282 3423
Fragments arranged by size:
3423 1896 1282
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2054 5188 5562 5708 387
Size: 1064 138 465 3134 374 146 1280
Fragments arranged by size:
3134 1280 1064 465 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2766 4664 5261 6048 6316 1239
Size: 393 1134 1898 597 787 268 1524
Fragments arranged by size:
1898 1524 1134 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2368 2368
Size: 6601
EcoRI G'AATT_C
Cuts at: 2374 3910 4288 2374
Size: 1536 378 4687
Fragments arranged by size:
4687 1536 378
EcoRV GAT'ATC
Cuts at: 3672 3672
Size: 6601
FspI TGC'GCA
Cuts at: 5107 5107
Size: 6601
HaeII r_GCGC'y
Cuts at: 1678 2789 5748 1678
Size: 1111 2959 2531
Fragments arranged by size:
2959 2531 1111
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2855 2887 3283 3315 3860
Size: 32 135 32 1283 32 396 32 545
Cuts at: 3860 3892 5242 5274 5316 5348 1373
Size: 32 1350 32 42 32 2626
Fragments arranged by size:
2626 1350 1283 545 396 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2656 3945 4191 669
Size: 988 281 718 1289 246 3079
Fragments arranged by size:
3079 1289 988 718 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6601
HpaI GTT'AAC
Cuts at: 2656 4191 2656
Size: 1535 5066
KpnI G_GTAC'C
Cuts at: 3892 3926 3892
Size: 34 6567
MfeI C'AATT_G
Cuts at: 2517 4200 2517
Size: 1683 4918
MluI A'CGCG_T
Cuts at: 6477 6477
Size: 6601
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2645 5715 5899 1305
Size: 1340 3070 184 2007
Fragments arranged by size:
3070 2007 1340 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4779
Fragments arranged by size:
4779 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2033 2130 2163 2423 3363 3635 3771
Size: 648 867 97 33 260 940 272 136
Cuts at: 3771 4559 4918 5077 518
Size: 788 359 159 2042
Fragments arranged by size:
2042 940 867 788 648 359 272 260
159 136 97 33
MspA1I CmG'CkG
Cuts at: 1606 2011 3378 3895 4017 4694 5842 6087 1606
Size: 405 1367 517 122 677 1148 245 2120
Fragments arranged by size:
2120 1367 1148 677 517 405 245 122
NcoI C'CATG_G
Cuts at: 513 1065 2125 2418 3407 3766 513
Size: 552 1060 293 989 359 3348
Fragments arranged by size:
3348 1060 989 552 359 293
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6601
NheI G'CTAG_C
Cuts at: 1052 2704 1052
Size: 1652 4949
NotI GC'GGCC_GC
Cuts at: 4048 4048
Size: 6601
NsiI A_TGCA'T
Cuts at: 3069 3069
Size: 6601
NspI r_CATG'y
Cuts at: 1132 1229 3959 5504 1132
Size: 97 2730 1545 2229
Fragments arranged by size:
2730 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6601
PciI A'CATG_T
Cuts at: 5500 5500
Size: 6601
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3108 3898 1108
Size: 2000 790 3811
Fragments arranged by size:
3811 2000 790
PmeI GTTT'AAAC
Cuts at: 3905 3905
Size: 6601
PmlI CAC'GTG
Cuts at: 3582 3582
Size: 6601
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4670 4702 6183 6215 4670
Size: 32 1481 32 5056
Fragments arranged by size:
5056 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2368 2368
Size: 6601
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6601
PsiI TTA'TAA
Cuts at: 2121 4171 2121
Size: 2050 4551
PstI C_TGCA'G
Cuts at: 830 3323 3953 830
Size: 2493 630 3478
Fragments arranged by size:
3478 2493 630
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6601
PvuII CAG'CTG
Cuts at: 3895 3895
Size: 6601
SalI G'TCGA_C
Cuts at: 1936 3943 1936
Size: 2007 4594
SapI GCTCTTCn'nnn_
Cuts at: 3249 3249
Size: 6601
SbfI CC_TGCA'GG
Cuts at: 3953 3953
Size: 6601
ScaI AGT'ACT
Cuts at: 4849 6561 4849
Size: 1712 4889
SfcI C'TryA_G
Cuts at: 826 1045 3024 3319 3949 5084 5765 5956 826
Size: 219 1979 295 630 1135 681 191 1471
Fragments arranged by size:
1979 1471 1135 681 630 295 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6601
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6601
SmaI CCC'GGG
Cuts at: 1872 3928 1872
Size: 2056 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4705 5606 5868 820
Size: 197 926 2762 901 262 1553
Fragments arranged by size:
2762 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4392 493
Size: 3899 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6601
SphI G_CATG'C
Cuts at: 1132 3959 1132
Size: 2827 3774
SspI AAT'ATT
Cuts at: 5 52 2977 3784 4525 5
Size: 47 2925 807 741 2081
Fragments arranged by size:
2925 2081 807 741 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6601
StyI C'CwwG_G
Cuts at: 513 1065 2125 2418 2659 3082 3407 3543 3590
Size: 552 1060 293 241 423 325 136 47
Cuts at: 3590 3766 4040 513
Size: 176 274 3074
Fragments arranged by size:
3074 1060 552 423 325 293 274 241
176 136 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2106 3400 3625 4628 4645 4798 4983
Size: 1255 526 1294 225 1003 17 153 185
Cuts at: 4983 5428 325
Size: 445 1498
Fragments arranged by size:
1498 1294 1255 1003 526 445 225 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3028 4847 6559
Size: 274 80 33 51 1443 1051 1819 1712
Cuts at: 6559 96
Size: 138
Fragments arranged by size:
1819 1712 1443 1051 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6561 6593 6561
Size: 32 6569
Tth111I GACn'n_nGTC
Cuts at: 1316 2186 1316
Size: 870 5731
XbaI T'CTAG_A
Cuts at: 3937 3937
Size: 6601
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2123 2123
Size: 6601
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6601
XmaI C'CCGG_G
Cuts at: 1870 3926 1870
Size: 2056 4545
XmnI GAAnn'nnTTC
Cuts at: 4730 4730
Size: 6601
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4409 276
Size: 53 83 186 936 2875 2468
Fragments arranged by size:
2875 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI Bsu36I EcoICRI EcoNI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI