Restriction map for
pFN21AA0942
(Circular) (Six-base) MAPSORT of: pFN21AA0942.seq Check: 680 from: 1 to: 6244
pFN21AA0942 6244 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3291 4054 278
Size: 53 83 186 936 1755 763 2468
Fragments arranged by size:
2468 1755 936 763 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3587 1937
Size: 1650 4594
Acc65I G'GTAC_C
Cuts at: 2132 3565 2132
Size: 1433 4811
AclI AA'CG_TT
Cuts at: 1563 4371 4744 1563
Size: 2808 373 3063
Fragments arranged by size:
3063 2808 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2288 2876 2934 3218 3239 3437 4307 5691
Size: 656 588 58 284 21 198 870 1384
Cuts at: 5691 5959 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 870 656 588 284 268 198
58 21
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6047
AflIII A'CryG_T
Cuts at: 1203 2991 3486 5143 6120 1203
Size: 1788 495 1657 977 1327
Fragments arranged by size:
1788 1657 1327 977 495
AgeI A'CCGG_T
Cuts at: 5075 5075
Size: 6244
AhdI GACnn_n'nnGTC
Cuts at: 2694 4973 2694
Size: 2279 3965
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6244
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6244
AlwNI CAG_nnn'CTG
Cuts at: 3987 5559 3987
Size: 1572 4672
ApaLI G'TGCA_C
Cuts at: 2942 4301 5457 2942
Size: 1359 1156 3729
Fragments arranged by size:
3729 1359 1156
ApoI r'AATT_y
Cuts at: 1493 2094 2237 2784 3553 3779 3931 6040 1493
Size: 601 143 547 769 226 152 2109 1697
Fragments arranged by size:
2109 1697 769 601 547 226 152 143
AseI AT'TA_AT
Cuts at: 160 1032 4798 160
Size: 872 3766 1606
Fragments arranged by size:
3766 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6244
AvaI C'yCGr_G
Cuts at: 1870 1943 3430 3569 1870
Size: 73 1487 139 4545
Fragments arranged by size:
4545 1487 139 73
BamHI G'GATC_C
Cuts at: 3574 3941 3574
Size: 367 5877
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2132 3565 5020 618
Size: 325 217 627 345 1433 1455 1842
Fragments arranged by size:
1842 1455 1433 627 345 325 217
BanII G_rGCy'C
Cuts at: 1391 2012 3047 3563 1391
Size: 621 1035 516 4072
Fragments arranged by size:
4072 1035 621 516
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2816 928
Size: 971 917 4356
Fragments arranged by size:
4356 971 917
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4435 4469 1903
Size: 34 2498 34 3678
Fragments arranged by size:
3678 2498 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3445 4136 5346 3445
Size: 691 1210 4343
Fragments arranged by size:
4343 1210 691
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6244
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4855 136
Size: 107 122 71 631 605 3183 1525
Fragments arranged by size:
3183 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6239 6239
Size: 6244
BlpI GC'TnA_GC
Cuts at: 3661 3661
Size: 6244
Bme1580I G_kGCm'C
Cuts at: 1790 2946 4305 5461 1790
Size: 1156 1359 1156 2573
Fragments arranged by size:
2573 1359 1156 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6244
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3975 4933 442
Size: 416 53 83 399 24 2558 958 1753
Fragments arranged by size:
2558 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6244
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3268 3268
Size: 6244
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3037 3341 4904 1239
Size: 1798 304 1563 2579
Fragments arranged by size:
2579 1798 1563 304
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2334 3347 4369 5234 5532 2334
Size: 1013 1022 865 298 3046
Fragments arranged by size:
3046 1022 1013 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2102 4907 882
Size: 617 603 2805 2219
Fragments arranged by size:
2805 2219 617 603
BsaAI yAC'GTr
Cuts at: 493 1204 4035 493
Size: 711 2831 2702
Fragments arranged by size:
2831 2702 711
BsaBI GATnn'nnATC
Cuts at: 6238 6238
Size: 6244
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3288 4051 4433 5068
Size: 53 83 186 936 1755 763 382 635
Cuts at: 5068 275
Size: 1451
Fragments arranged by size:
1755 1451 936 763 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4677 5075 5349 5496 5907 4677
Size: 398 274 147 411 5014
Fragments arranged by size:
5014 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6019
Fragments arranged by size:
6019 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2675 3203 1186
Size: 1489 528 4227
Fragments arranged by size:
4227 1489 528
BseYI C'CCAG_C
Cuts at: 2042 5447 5943 2042
Size: 3405 496 2343
Fragments arranged by size:
3405 2343 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3087 1163
Size: 573 1351 4320
Fragments arranged by size:
4320 1351 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3694 4455 5483 1540
Size: 275 186 1693 761 1028 2301
Fragments arranged by size:
2301 1693 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2090 2946 3249 4305 4390 5461 2090
Size: 856 303 1056 85 1071 2873
Fragments arranged by size:
2873 1071 1056 856 303 85
BsmI GAATG_Cn'
Cuts at: 2327 3357 3755 3848 2327
Size: 1030 398 93 4723
Fragments arranged by size:
4723 1030 398 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2090 2946 3047 3249 3563 4305
Size: 399 222 78 856 101 202 314 742
Cuts at: 4305 4390 5461 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 856 742 399 314 222 202
101 85 78
BspEI T'CCGG_A
Cuts at: 5907 5907
Size: 6244
BspHI T'CATG_A
Cuts at: 2737 4133 5863 2737
Size: 1396 1730 3118
Fragments arranged by size:
3118 1730 1396
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3599 844
Size: 2755 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3252 4131 1394
Size: 429 1429 879 3507
Fragments arranged by size:
3507 1429 879 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2044 2149 2412 2434 2517 4739 4913
Size: 1183 796 105 263 22 83 2222 174
Cuts at: 4913 65
Size: 1396
Fragments arranged by size:
2222 1396 1183 796 263 174 105 83
22
BsrFI r'CCGG_y
Cuts at: 1700 1810 4888 5075 1700
Size: 110 3078 187 2869
Fragments arranged by size:
3078 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2580 2757 96
Size: 2484 177 3583
Fragments arranged by size:
3583 2484 177
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6244
BssSI C'ACGA_G
Cuts at: 4304 5316 4304
Size: 1012 5232
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6244
BstBI TT'CG_AA
Cuts at: 5048 5048
Size: 6244
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6244
BstXI CCAn_nnnn'nTGG
Cuts at: 2164 2489 3238 2164
Size: 325 749 5170
Fragments arranged by size:
5170 749 325
BstYI r'GATC_y
Cuts at: 1972 2563 3574 3941 4326 4343 5795 5881 5893
Size: 591 1011 367 385 17 1452 86 12
Cuts at: 5893 6239 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1011 591 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2967 513
Size: 552 939 963 3790
Fragments arranged by size:
3790 963 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5505
Fragments arranged by size:
5505 683 56
BtsI GCAGTG_nn'
Cuts at: 3465 3760 4554 4574 3465
Size: 295 794 20 5135
Fragments arranged by size:
5135 794 295 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6244
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6209
DraI TTT'AAA
Cuts at: 2323 3548 2323
Size: 1225 5019
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3020 3272 5251 809
Size: 507 1704 252 1979 1802
Fragments arranged by size:
1979 1802 1704 507 252
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2166 2479 3240
Size: 54 1425 50 275 18 336 313 761
Cuts at: 3240 3691 4580 6209 8
Size: 451 889 1629 43
Fragments arranged by size:
1629 1425 889 761 451 336 313 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3691 1537
Size: 275 1879 4090
Fragments arranged by size:
4090 1879 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2017 2622 2910 4174 1353
Size: 664 605 288 1264 3423
Fragments arranged by size:
3423 1264 664 605 288
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4831 5205 5351 387
Size: 1064 138 3242 374 146 1280
Fragments arranged by size:
3242 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2288 2876 2934 3037 3218 3239 3341
Size: 393 656 588 58 103 181 21 102
Cuts at: 3341 3437 4307 4904 5691 5959 1239
Size: 96 870 597 787 268 1524
Fragments arranged by size:
1524 870 787 656 597 588 393 268
181 103 102 96 58 21
EcoRI G'AATT_C
Cuts at: 3553 3931 3553
Size: 378 5866
FspI TGC'GCA
Cuts at: 4750 4750
Size: 6244
HaeII r_GCGC'y
Cuts at: 1678 5391 1678
Size: 3713 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2284 2316 2491 2523 2680
Size: 32 135 32 712 32 175 32 157
Cuts at: 2680 2712 3487 3519 4885 4917 4959 4991 1373
Size: 32 775 32 1366 32 42 32 2626
Fragments arranged by size:
2626 1366 775 712 175 157 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3588 3834 669
Size: 988 281 1650 246 3079
Fragments arranged by size:
3079 1650 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2603 748
Size: 1855 4389
HpaI GTT'AAC
Cuts at: 3834 3834
Size: 6244
KpnI G_GTAC'C
Cuts at: 2136 3569 2136
Size: 1433 4811
MfeI C'AATT_G
Cuts at: 3079 3843 3079
Size: 764 5480
MluI A'CGCG_T
Cuts at: 6120 6120
Size: 6244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5358 5542 1305
Size: 4053 184 2007
Fragments arranged by size:
4053 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2168 2481 10
Size: 54 1425 343 336 313 3773
Fragments arranged by size:
3773 1425 343 336 313 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4202 4561 4720 518
Size: 648 3036 359 159 2042
Fragments arranged by size:
3036 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2185 3092 3660 4337 5485 5730 1606
Size: 579 907 568 677 1148 245 2120
Fragments arranged by size:
2120 1148 907 677 579 568 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4753
Fragments arranged by size:
4753 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6244
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6244
NotI GC'GGCC_GC
Cuts at: 3691 3691
Size: 6244
NspI r_CATG'y
Cuts at: 1132 1229 3602 5147 1132
Size: 97 2373 1545 2229
Fragments arranged by size:
2373 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6244
PciI A'CATG_T
Cuts at: 5143 5143
Size: 6244
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2953 3319 1108
Size: 1845 366 4033
Fragments arranged by size:
4033 1845 366
PmeI GTTT'AAAC
Cuts at: 3548 3548
Size: 6244
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4313 4345 5826 5858 4313
Size: 32 1481 32 4699
Fragments arranged by size:
4699 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6244
PsiI TTA'TAA
Cuts at: 3814 3814
Size: 6244
PspXI vC'TCGA_Gb
Cuts at: 3430 3430
Size: 6244
PstI C_TGCA'G
Cuts at: 830 3412 3596 830
Size: 2582 184 3478
Fragments arranged by size:
3478 2582 184
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6244
PvuII CAG'CTG
Cuts at: 2185 3092 2185
Size: 907 5337
SalI G'TCGA_C
Cuts at: 1936 3586 1936
Size: 1650 4594
SapI GCTCTTCn'nnn_
Cuts at: 2017 2017
Size: 6244
SbfI CC_TGCA'GG
Cuts at: 3596 3596
Size: 6244
ScaI AGT'ACT
Cuts at: 2656 2710 4492 6204 2656
Size: 54 1782 1712 2696
Fragments arranged by size:
2696 1782 1712 54
SfcI C'TryA_G
Cuts at: 826 1045 3408 3592 4727 5408 5599 826
Size: 219 2363 184 1135 681 191 1471
Fragments arranged by size:
2363 1471 1135 681 219 191 184
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6244
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6244
SmaI CCC'GGG
Cuts at: 1872 3571 1872
Size: 1699 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2349 3362 3430 4348 5249 5511
Size: 197 926 406 1013 68 918 901 262
Cuts at: 5511 820
Size: 1553
Fragments arranged by size:
1553 1013 926 918 901 406 262 197
68
SnaBI TAC'GTA
Cuts at: 493 4035 493
Size: 3542 2702
SpeI A'CTAG_T
Cuts at: 152 2248 152
Size: 2096 4148
SphI G_CATG'C
Cuts at: 1132 3602 1132
Size: 2470 3774
SspI AAT'ATT
Cuts at: 5 52 2488 4168 5
Size: 47 2436 1680 2081
Fragments arranged by size:
2436 2081 1680 47
StuI AGG'CCT
Cuts at: 1517 2906 1517
Size: 1389 4855
StyI C'CwwG_G
Cuts at: 513 1065 2004 2221 2559 2961 3282 3683 513
Size: 552 939 217 338 402 321 401 3074
Fragments arranged by size:
3074 939 552 402 401 338 321 217
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4271 4288 4441 4626 5071 325
Size: 1255 2691 17 153 185 445 1498
Fragments arranged by size:
2691 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2580 2654 2708
Size: 274 80 33 51 1443 603 74 54
Cuts at: 2708 2757 2851 3297 4490 6202 96
Size: 49 94 446 1193 1712 138
Fragments arranged by size:
1712 1443 1193 603 446 274 138 94
80 74 54 51 49 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6204 6236 6204
Size: 32 6212
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6244
XbaI T'CTAG_A
Cuts at: 3580 3580
Size: 6244
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2143 2143
Size: 6244
XhoI C'TCGA_G
Cuts at: 1943 3430 1943
Size: 1487 4757
XmaI C'CCGG_G
Cuts at: 1870 3569 1870
Size: 1699 4545
XmnI GAAnn'nnTTC
Cuts at: 4373 4373
Size: 6244
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3289 4052 276
Size: 53 83 186 936 1755 763 2468
Fragments arranged by size:
2468 1755 936 763 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspXI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PsrI RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI