Restriction map for
pFN21AA0938
(Circular) (Six-base) MAPSORT of: pFN21AA0938.seq Check: 9810 from: 1 to: 10231
pFN21AA0938 10231 bp
With 182 enzymes: SgfI *
February 20, 2021 22:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4262 4262
Size: 10231
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6253 8041 278
Size: 53 83 186 936 4717 1788 2468
Fragments arranged by size:
4717 2468 1788 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2710 2846 7574 1937
Size: 773 136 4728 4594
Fragments arranged by size:
4728 4594 773 136
Acc65I G'GTAC_C
Cuts at: 2804 7552 2804
Size: 4748 5483
AclI AA'CG_TT
Cuts at: 1563 8358 8731 1563
Size: 6795 373 3063
Fragments arranged by size:
6795 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2054 2388 2439 2741 3432 3603 4802 5169
Size: 422 334 51 302 691 171 1199 367
Cuts at: 5169 5393 5718 5804 7190 7295 8294 9678 9946
Size: 224 325 86 1386 105 999 1384 268
Cuts at: 9946 1632
Size: 1917
Fragments arranged by size:
1917 1386 1384 1199 999 691 422 367
334 325 302 268 224 171 105 86
51
AflII C'TTAA_G
Cuts at: 820 1017 4477 820
Size: 197 3460 6574
Fragments arranged by size:
6574 3460 197
AflIII A'CryG_T
Cuts at: 1203 2379 2889 2934 9130 10107 1203
Size: 1176 510 45 6196 977 1327
Fragments arranged by size:
6196 1327 1176 977 510 45
AgeI A'CCGG_T
Cuts at: 5925 9062 5925
Size: 3137 7094
AhdI GACnn_n'nnGTC
Cuts at: 6655 8960 6655
Size: 2305 7926
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 10231
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2031 1184
Size: 847 9384
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3203 3235 3203
Size: 32 10199
AlwNI CAG_nnn'CTG
Cuts at: 2239 2412 4185 4699 5759 5942 6056 7974 9546
Size: 173 1773 514 1060 183 114 1918 1572
Cuts at: 9546 2239
Size: 2924
Fragments arranged by size:
2924 1918 1773 1572 1060 514 183 173
114
ApaI G_GGCC'C
Cuts at: 4944 4944
Size: 10231
ApaLI G'TGCA_C
Cuts at: 3528 4191 8288 9444 3528
Size: 663 4097 1156 4315
Fragments arranged by size:
4315 4097 1156 663
ApoI r'AATT_y
Cuts at: 1493 2587 3789 4915 5383 7540 7766 7918 10027
Size: 1094 1202 1126 468 2157 226 152 2109
Cuts at: 10027 1493
Size: 1697
Fragments arranged by size:
2157 2109 1697 1202 1126 1094 468 226
152
AseI AT'TA_AT
Cuts at: 160 1032 8785 160
Size: 872 7753 1606
Fragments arranged by size:
7753 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10231
AvaI C'yCGr_G
Cuts at: 1870 1943 2833 3090 3419 3542 4069 4937 7453
Size: 73 890 257 329 123 527 868 2516
Cuts at: 7453 7556 1870
Size: 103 4545
Fragments arranged by size:
4545 2516 890 868 527 329 257 123
103 73
BamHI G'GATC_C
Cuts at: 4214 6194 7561 7928 4214
Size: 1980 1367 367 6517
Fragments arranged by size:
6517 1980 1367 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2804 3499 4262 7552 9007
Size: 325 217 627 1017 695 763 3290 1455
Cuts at: 9007 618
Size: 1842
Fragments arranged by size:
3290 1842 1455 1017 763 695 627 325
217
BanII G_rGCy'C
Cuts at: 1391 4622 4910 4944 5069 5796 6308 6732 6773
Size: 3231 288 34 125 727 512 424 41
Cuts at: 6773 7277 7301 7550 1391
Size: 504 24 249 4072
Fragments arranged by size:
4072 3231 727 512 504 424 288 249
125 41 34 24
BbeI G_GCGC'C
Cuts at: 3503 3503
Size: 10231
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2310 2538 2603 2931 3395 3420 5464
Size: 971 411 228 65 328 464 25 2044
Cuts at: 5464 7037 7196 7503 928
Size: 1573 159 307 3656
Fragments arranged by size:
3656 2044 1573 971 464 411 328 307
228 159 65 25
BbvCI CC'TCA_GC
Cuts at: 3275 4643 5760 5840 3275
Size: 1368 1117 80 7666
Fragments arranged by size:
7666 1368 1117 80
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 8422 8456 1903
Size: 34 50 34 6401 34 3678
Fragments arranged by size:
6401 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4233 4573 6380 8123 9333 4233
Size: 340 1807 1743 1210 5131
Fragments arranged by size:
5131 1807 1743 1210 340
BclI T'GATC_A
Cuts at: 1548 3738 5860 1548
Size: 2190 2122 5919
Fragments arranged by size:
5919 2190 2122
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3508 3963 4653
Size: 107 122 71 631 605 1836 455 690
Cuts at: 4653 5399 5965 8842 136
Size: 746 566 2877 1525
Fragments arranged by size:
2877 1836 1525 746 690 631 605 566
455 122 107 71
BglII A'GATC_T
Cuts at: 3611 4724 5443 10226 3611
Size: 1113 719 4783 3616
Fragments arranged by size:
4783 3616 1113 719
BlpI GC'TnA_GC
Cuts at: 2257 4415 5704 5955 7276 7648 2257
Size: 2158 1289 251 1321 372 4840
Fragments arranged by size:
4840 2158 1321 1289 372 251
Bme1580I G_kGCm'C
Cuts at: 1790 3532 4195 4944 8292 9448 1790
Size: 1742 663 749 3348 1156 2573
Fragments arranged by size:
3348 2573 1742 1156 749 663
BmgBI CAC'GTC
Cuts at: 1318 7035 1318
Size: 5717 4514
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3791 6728 7962
Size: 416 53 83 399 24 2374 2937 1234
Cuts at: 7962 8920 442
Size: 958 1753
Fragments arranged by size:
2937 2374 1753 1234 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 6324 1056
Size: 5268 4963
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2218 3313 4746 6746 2218
Size: 1095 1433 2000 5703
Fragments arranged by size:
5703 2000 1433 1095
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2663 2749 3490 4388 4480 4830 5054 5852
Size: 1424 86 741 898 92 350 224 798
Cuts at: 5852 7205 8891 1239
Size: 1353 1686 2579
Fragments arranged by size:
2579 1686 1424 1353 898 798 741 350
224 92 86
Bpu10I CC'TnA_GC
Cuts at: 3275 4643 5278 5760 5840 3275
Size: 1368 635 482 80 7666
Fragments arranged by size:
7666 1368 635 482 80
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2599 5968 8356 9221 9519 2599
Size: 3369 2388 865 298 3311
Fragments arranged by size:
3369 3311 2388 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2116 3176 3305 4205 4538 8894 882
Size: 617 617 1060 129 900 333 4356 2219
Fragments arranged by size:
4356 2219 1060 900 617 617 333 129
BsaAI yAC'GTr
Cuts at: 493 1204 8022 493
Size: 711 6818 2702
Fragments arranged by size:
6818 2702 711
BsaBI GATnn'nnATC
Cuts at: 10225 10225
Size: 10231
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3500 6250 8038 8420
Size: 53 83 186 936 1967 2750 1788 382
Cuts at: 8420 9055 275
Size: 635 1451
Fragments arranged by size:
2750 1967 1788 1451 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2034 4382 5883 5925 8664 9062 9336 9483 9894
Size: 2348 1501 42 2739 398 274 147 411
Cuts at: 9894 2034
Size: 2371
Fragments arranged by size:
2739 2371 2348 1501 411 398 274 147
42
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2369 2399 4361 4391 5599
Size: 30 165 30 966 30 1962 30 1208
Cuts at: 5599 5629 1178
Size: 30 5780
Fragments arranged by size:
5780 1962 1208 966 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2134 2438 2483 3975 5986 1186
Size: 948 304 45 1492 2011 5431
Fragments arranged by size:
5431 2011 1492 948 304 45
BseYI C'CCAG_C
Cuts at: 2279 2412 2488 2881 3141 4016 4331 4501 4943
Size: 133 76 393 260 875 315 170 442
Cuts at: 4943 4969 5007 7300 9434 9930 2279
Size: 26 38 2293 2134 496 2580
Fragments arranged by size:
2580 2293 2134 875 496 442 393 315
260 170 133 76 38 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2252 4275 6724 7330 1163
Size: 573 516 2023 2449 606 4064
Fragments arranged by size:
4064 2449 2023 606 573 516
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7681 8442 9470 1540
Size: 275 186 5680 761 1028 2301
Fragments arranged by size:
5680 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3532 4195 4622 5416 5796 6308 6732 7277 7460
Size: 663 427 794 380 512 424 545 183
Cuts at: 7460 8292 8377 9448 3532
Size: 832 85 1071 4315
Fragments arranged by size:
4315 1071 832 794 663 545 512 427
424 380 183 85
BsmI GAATG_Cn'
Cuts at: 3948 5464 5753 7742 7835 3948
Size: 1516 289 1989 93 6344
Fragments arranged by size:
6344 1989 1516 289 93
BsmBI CGTCTCn'nnnn_
Cuts at: 6257 6257
Size: 10231
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3532 4195 4622 4910 4944 5069 5416
Size: 399 1742 663 427 288 34 125 347
Cuts at: 5416 5796 6308 6732 6773 7277 7301 7460 7550
Size: 380 512 424 41 504 24 159 90
Cuts at: 7550 8292 8377 9448 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1742 1071 742 663 512 504 427
424 399 380 347 288 159 125 90
85 41 34 24
BspEI T'CCGG_A
Cuts at: 2034 9894 2034
Size: 7860 2371
BspHI T'CATG_A
Cuts at: 4704 8120 9850 4704
Size: 3416 1730 5085
Fragments arranged by size:
5085 3416 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4262 7586 844
Size: 3418 3324 3489
Fragments arranged by size:
3489 3418 3324
BsrBI CCG'CTC
Cuts at: 1394 1823 2197 8118 1394
Size: 429 374 5921 3507
Fragments arranged by size:
5921 3507 429 374
BsrDI GCAATG_nn'
Cuts at: 65 1248 6315 8726 8900 65
Size: 1183 5067 2411 174 1396
Fragments arranged by size:
5067 2411 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5925 8875 9062 1700
Size: 110 4115 2950 187 2869
Fragments arranged by size:
4115 2950 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 10231
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10231
BssSI C'ACGA_G
Cuts at: 4535 4615 4685 5046 8291 9303 4535
Size: 80 70 361 3245 1012 5463
Fragments arranged by size:
5463 3245 1012 361 80 70
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 10231
BstBI TT'CG_AA
Cuts at: 4249 6952 9035 4249
Size: 2703 2083 5445
Fragments arranged by size:
5445 2703 2083
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 10231
BstXI CCAn_nnnn'nTGG
Cuts at: 5015 5032 5690 5015
Size: 17 658 9556
Fragments arranged by size:
9556 658 17
BstYI r'GATC_y
Cuts at: 1972 3611 3971 4214 4724 5443 5865 6194 6347
Size: 1639 360 243 510 719 422 329 153
Cuts at: 6347 7150 7237 7405 7561 7928 8313 8330 9782
Size: 803 87 168 156 367 385 17 1452
Cuts at: 9782 9868 9880 10226 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1639 1452 803 719 510 422 385
367 360 346 329 243 168 156 153
87 86 17 12
BstZ17I GTA'TAC
Cuts at: 2847 2847
Size: 10231
Bsu36I CC'TnA_GG
Cuts at: 3327 3664 7290 3327
Size: 337 3626 6268
Fragments arranged by size:
6268 3626 337
BtgI C'CryG_G
Cuts at: 513 1065 2127 3364 7116 7229 7268 513
Size: 552 1062 1237 3752 113 39 3476
Fragments arranged by size:
3752 3476 1237 1062 552 113 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9492
Fragments arranged by size:
9492 683 56
BtsI GCAGTG_nn'
Cuts at: 2806 2929 3172 3643 3754 4408 5527 6124 6712
Size: 123 243 471 111 654 1119 597 588
Cuts at: 6712 7197 7747 8541 8561 2806
Size: 485 550 794 20 4476
Fragments arranged by size:
4476 1119 794 654 597 588 550 485
471 243 123 111 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10231
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4255 4290 6277 6312 529
Size: 35 3691 35 1987 35 4448
Fragments arranged by size:
4448 3691 1987 35 35 35
DraI TTT'AAA
Cuts at: 7535 7535
Size: 10231
DraIII CAC_nnn'GTG
Cuts at: 3121 3121
Size: 10231
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9238 809
Size: 507 7922 1802
Fragments arranged by size:
7922 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3955 5293 5966
Size: 54 1425 50 275 18 2125 1338 673
Cuts at: 5966 6112 7678 8567 10196 8
Size: 146 1566 889 1629 43
Fragments arranged by size:
2125 1629 1566 1425 1338 889 673 275
146 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7678 1537
Size: 275 5866 4090
Fragments arranged by size:
5866 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2323 2415 2589 3469 4442 4770 4987 5107
Size: 970 92 174 880 973 328 217 120
Cuts at: 5107 5634 5780 6004 8161 1353
Size: 527 146 224 2157 3423
Fragments arranged by size:
3423 2157 973 970 880 527 328 224
217 174 146 120 92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8818 9192 9338 387
Size: 1064 138 7229 374 146 1280
Fragments arranged by size:
7229 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4620 5794 6306 6730 7275 4620
Size: 1174 512 424 545 7576
Fragments arranged by size:
7576 1174 545 512 424
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2388 2439 2663 2741 2749 3432
Size: 393 422 334 51 224 78 8 683
Cuts at: 3432 3490 3603 4388 4480 4802 4830 5054 5169
Size: 58 113 785 92 322 28 224 115
Cuts at: 5169 5393 5718 5804 5852 7190 7205 7295 8294
Size: 224 325 86 48 1338 15 90 999
Cuts at: 8294 8891 9678 9946 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1338 999 787 785 683 597 422
393 334 325 322 268 224 224 224
115 113 92 90 86 78 58 51
48 28 15 8
EcoNI CCTnn'n_nnAGG
Cuts at: 3957 3957
Size: 10231
EcoO109I rG'GnC_Cy
Cuts at: 5836 5836
Size: 10231
EcoRI G'AATT_C
Cuts at: 5383 7540 7918 5383
Size: 2157 378 7696
Fragments arranged by size:
7696 2157 378
EcoRV GAT'ATC
Cuts at: 2980 6784 6947 2980
Size: 3804 163 6264
Fragments arranged by size:
6264 3804 163
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3633 4158 3633
Size: 525 9706
FspI TGC'GCA
Cuts at: 2135 8737 2135
Size: 6602 3629
FspAI rTGC'GCAy
Cuts at: 2135 2135
Size: 10231
HaeII r_GCGC'y
Cuts at: 1678 2203 3503 4069 4185 9378 1678
Size: 525 1300 566 116 5193 2531
Fragments arranged by size:
5193 2531 1300 566 525 116
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2303 2335 2348 2380 2443
Size: 32 135 32 731 32 13 32 63
Cuts at: 2443 2475 2588 2620 2759 2791 2856 2888 3154
Size: 32 113 32 139 32 65 32 266
Cuts at: 3154 3186 4220 4252 4361 4393 4716 4748 5522
Size: 32 1034 32 109 32 323 32 774
Cuts at: 5522 5554 5687 5719 6158 6190 6505 6537 6641
Size: 32 133 32 439 32 315 32 104
Cuts at: 6641 6673 7024 7056 7186 7218 8872 8904 8946
Size: 32 351 32 130 32 1654 32 42
Cuts at: 8946 8978 1373
Size: 32 2626
Fragments arranged by size:
2626 1654 1034 774 731 439 351 323
315 266 139 135 133 130 113 109
104 65 63 42 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2711 4087 7575 7821 669
Size: 988 281 773 1376 3488 246 3079
Fragments arranged by size:
3488 3079 1376 988 773 281 246
HindIII A'AGCT_T
Cuts at: 748 3438 3564 4474 748
Size: 2690 126 910 6505
Fragments arranged by size:
6505 2690 910 126
HpaI GTT'AAC
Cuts at: 7821 7821
Size: 10231
KasI G'GCGC_C
Cuts at: 3499 3499
Size: 10231
KpnI G_GTAC'C
Cuts at: 2808 7556 2808
Size: 4748 5483
MfeI C'AATT_G
Cuts at: 6630 6802 7830 6630
Size: 172 1028 9031
Fragments arranged by size:
9031 1028 172
MluI A'CGCG_T
Cuts at: 10107 10107
Size: 10231
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2166 3264 4020 6376 9345 9529 1305
Size: 861 1098 756 2356 2969 184 2007
Fragments arranged by size:
2969 2356 2007 1098 861 756 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 8409
Fragments arranged by size:
8409 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2463 2720 3369 5366 6108 6416 7267
Size: 648 1297 257 649 1997 742 308 851
Cuts at: 7267 8189 8548 8707 518
Size: 922 359 159 2042
Fragments arranged by size:
2042 1997 1297 922 851 742 649 648
359 308 257 159
MspA1I CmG'CkG
Cuts at: 1606 2011 3141 3274 4877 5147 5321 5782 7315
Size: 405 1130 133 1603 270 174 461 1533
Cuts at: 7315 7647 8324 9472 9717 1606
Size: 332 677 1148 245 2120
Fragments arranged by size:
2120 1603 1533 1148 1130 677 461 405
332 270 245 174 133
NarI GG'CG_CC
Cuts at: 3500 3500
Size: 10231
NcoI C'CATG_G
Cuts at: 513 1065 3364 7116 7229 7268 513
Size: 552 2299 3752 113 39 3476
Fragments arranged by size:
3752 3476 2299 552 113 39
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10231
NheI G'CTAG_C
Cuts at: 1052 6320 1052
Size: 5268 4963
NotI GC'GGCC_GC
Cuts at: 7678 7678
Size: 10231
NspI r_CATG'y
Cuts at: 1132 1229 2748 2893 2938 3484 3884 7589 9134
Size: 97 1519 145 45 546 400 3705 1545
Cuts at: 9134 1132
Size: 2229
Fragments arranged by size:
3705 2229 1545 1519 546 400 145 97
45
PasI CC'CwG_GG
Cuts at: 1348 2779 1348
Size: 1431 8800
PciI A'CATG_T
Cuts at: 2889 2934 9130 2889
Size: 45 6196 3990
Fragments arranged by size:
6196 3990 45
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2727 1108
Size: 1619 8612
PfoI T'CCnGG_A
Cuts at: 3467 5868 3467
Size: 2401 7830
PmeI GTTT'AAAC
Cuts at: 7535 7535
Size: 10231
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8300 8332 9813 9845 8300
Size: 32 1481 32 8686
Fragments arranged by size:
8686 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10231
PsiI TTA'TAA
Cuts at: 4472 7801 4472
Size: 3329 6902
PspOMI G'GGCC_C
Cuts at: 4940 4940
Size: 10231
PspXI vC'TCGA_Gb
Cuts at: 2833 4069 7453 2833
Size: 1236 3384 5611
Fragments arranged by size:
5611 3384 1236
PstI C_TGCA'G
Cuts at: 830 4570 4876 5781 7583 830
Size: 3740 306 905 1802 3478
Fragments arranged by size:
3740 3478 1802 905 306
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10231
PvuII CAG'CTG
Cuts at: 3141 3274 4877 5147 5782 7315 3141
Size: 133 1603 270 635 1533 6057
Fragments arranged by size:
6057 1603 1533 635 270 133
SacI G_AGCT'C
Cuts at: 4622 5796 6308 6732 7277 4622
Size: 1174 512 424 545 7576
Fragments arranged by size:
7576 1174 545 512 424
SalI G'TCGA_C
Cuts at: 1936 2709 7573 1936
Size: 773 4864 4594
Fragments arranged by size:
4864 4594 773
SapI GCTCTTCn'nnn_
Cuts at: 5107 5634 5780 5107
Size: 527 146 9558
Fragments arranged by size:
9558 527 146
SbfI CC_TGCA'GG
Cuts at: 7583 7583
Size: 10231
ScaI AGT'ACT
Cuts at: 8479 10191 8479
Size: 1712 8519
SfcI C'TryA_G
Cuts at: 826 1045 4461 4566 4872 5142 5777 7579 8714
Size: 219 3416 105 306 270 635 1802 1135
Cuts at: 8714 9395 9586 826
Size: 681 191 1471
Fragments arranged by size:
3416 1802 1471 1135 681 635 306 270
219 191 105
SfiI GGCCn_nnn'nGGCC
Cuts at: 3963 3963
Size: 10231
SfoI GGC'GCC
Cuts at: 3501 3501
Size: 10231
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10231
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10231
SmaI CCC'GGG
Cuts at: 1872 3092 3544 4939 7558 1872
Size: 1220 452 1395 2619 4545
Fragments arranged by size:
4545 2619 1395 1220 452
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2578 2833 4069 4477 5983 7453
Size: 197 926 635 255 1236 408 1506 1470
Cuts at: 7453 8335 9236 9498 820
Size: 882 901 262 1553
Fragments arranged by size:
1553 1506 1470 1236 926 901 882 635
408 262 255 197
SnaBI TAC'GTA
Cuts at: 493 8022 493
Size: 7529 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10231
SphI G_CATG'C
Cuts at: 1132 7589 1132
Size: 6457 3774
SspI AAT'ATT
Cuts at: 5 52 8155 5
Size: 47 8103 2081
Fragments arranged by size:
8103 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 10231
StyI C'CwwG_G
Cuts at: 513 1065 2163 2983 3364 3490 4266 6295 7116
Size: 552 1098 820 381 126 776 2029 821
Cuts at: 7116 7229 7268 7670 513
Size: 113 39 402 3074
Fragments arranged by size:
3074 2029 1098 821 820 776 552 402
381 126 113 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2480 2771 2945 4255 6559 7240 8258
Size: 1255 900 291 174 1310 2304 681 1018
Cuts at: 8258 8275 8428 8613 9058 325
Size: 17 153 185 445 1498
Fragments arranged by size:
2304 1498 1310 1255 1018 900 681 445
291 185 174 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 8477 10189 96
Size: 274 80 33 51 1443 6500 1712 138
Fragments arranged by size:
6500 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5599 5631 10191 10223 5599
Size: 32 4560 32 5607
Fragments arranged by size:
5607 4560 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2602 3394 7004 1316
Size: 1286 792 3610 4543
Fragments arranged by size:
4543 3610 1286 792
XbaI T'CTAG_A
Cuts at: 2497 6871 7133 7567 2497
Size: 4374 262 434 5161
Fragments arranged by size:
5161 4374 434 262
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3371 5513 6643 3371
Size: 2142 1130 6959
Fragments arranged by size:
6959 2142 1130
XhoI C'TCGA_G
Cuts at: 1943 2833 4069 7453 1943
Size: 890 1236 3384 4721
Fragments arranged by size:
4721 3384 1236 890
XmaI C'CCGG_G
Cuts at: 1870 3090 3542 4937 7556 1870
Size: 1220 452 1395 2619 4545
Fragments arranged by size:
4545 2619 1395 1220 452
XmnI GAAnn'nnTTC
Cuts at: 5091 8360 5091
Size: 3269 6962
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6251 8039 276
Size: 53 83 186 936 4717 1788 2468
Fragments arranged by size:
4717 2468 1788 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI FseI NaeI
NgoMIV NruI NsiI PacI PmlI PpuMI PsrI RsrII
SacII SanDI SexAI SrfI SwaI