Restriction map for
pFN21AA0931
(Circular) (Six-base) MAPSORT of: pFN21AA0931.seq Check: 2098 from: 1 to: 8473
pFN21AA0931 8473 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6283 278
Size: 53 83 186 936 4747 2468
Fragments arranged by size:
4747 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3957 5310 5816 1937
Size: 2020 1353 506 4594
Fragments arranged by size:
4594 2020 1353 506
Acc65I G'GTAC_C
Cuts at: 2537 5794 2537
Size: 3257 5216
AclI AA'CG_TT
Cuts at: 1563 6600 6973 1563
Size: 5037 373 3063
Fragments arranged by size:
5037 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2300 2927 3108 3127 3719 4540 6536 7920
Size: 668 627 181 19 592 821 1996 1384
Cuts at: 7920 8188 1632
Size: 268 1917
Fragments arranged by size:
1996 1917 1384 821 668 627 592 268
181 19
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8276
AflIII A'CryG_T
Cuts at: 1203 7372 8349 1203
Size: 6169 977 1327
Fragments arranged by size:
6169 1327 977
AgeI A'CCGG_T
Cuts at: 7304 7304
Size: 8473
AhdI GACnn_n'nnGTC
Cuts at: 7202 7202
Size: 8473
AleI CACnn'nnGTG
Cuts at: 1166 3332 5582 1166
Size: 2166 2250 4057
Fragments arranged by size:
4057 2250 2166
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3223 4534 5468 1184
Size: 2039 1311 934 4189
Fragments arranged by size:
4189 2039 1311 934
AlwNI CAG_nnn'CTG
Cuts at: 4790 4958 6216 7788 4790
Size: 168 1258 1572 5475
Fragments arranged by size:
5475 1572 1258 168
ApaLI G'TGCA_C
Cuts at: 4843 6530 7686 4843
Size: 1687 1156 5630
Fragments arranged by size:
5630 1687 1156
ApoI r'AATT_y
Cuts at: 1493 2894 3126 3150 3633 5782 6008 6160 8269
Size: 1401 232 24 483 2149 226 152 2109
Cuts at: 8269 1493
Size: 1697
Fragments arranged by size:
2149 2109 1697 1401 483 232 226 152
24
AseI AT'TA_AT
Cuts at: 160 1032 7027 160
Size: 872 5995 1606
Fragments arranged by size:
5995 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8473
AvaI C'yCGr_G
Cuts at: 1870 1943 2058 2786 3719 5711 5798 1870
Size: 73 115 728 933 1992 87 4545
Fragments arranged by size:
4545 1992 933 728 115 87 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4322 4355 4322
Size: 33 8440
BamHI G'GATC_C
Cuts at: 4686 5415 5803 6170 4686
Size: 729 388 367 6989
Fragments arranged by size:
6989 729 388 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2537 5794 7249 618
Size: 325 217 627 750 3257 1455 1842
Fragments arranged by size:
3257 1842 1455 750 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2668 2985 4284 4421 4944 5724 5748 5792
Size: 1277 317 1299 137 523 780 24 44
Cuts at: 5792 1391
Size: 4072
Fragments arranged by size:
4072 1299 1277 780 523 317 137 44
24
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4546 5428 928
Size: 971 2647 882 3973
Fragments arranged by size:
3973 2647 971 882
BbvCI CC'TCA_GC
Cuts at: 3419 3419
Size: 8473
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6664 6698 1903
Size: 34 4727 34 3678
Fragments arranged by size:
4727 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6365 7575 6365
Size: 1210 7263
BclI T'GATC_A
Cuts at: 1548 4105 1548
Size: 2557 5916
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5149 7084 136
Size: 107 122 71 631 605 3477 1935 1525
Fragments arranged by size:
3477 1935 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2964 8468 2964
Size: 5504 2969
BlpI GC'TnA_GC
Cuts at: 2514 2656 5890 2514
Size: 142 3234 5097
Fragments arranged by size:
5097 3234 142
Bme1580I G_kGCm'C
Cuts at: 1790 4542 4847 5544 5589 6534 7690 1790
Size: 2752 305 697 45 945 1156 2573
Fragments arranged by size:
2752 2573 1156 945 697 305 45
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8473
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3571 6204 7162
Size: 416 53 83 399 24 2154 2633 958
Cuts at: 7162 442
Size: 1753
Fragments arranged by size:
2633 2154 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8473
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2259 3329 4174 4583 5203 5360 7133 1239
Size: 1020 1070 845 409 620 157 1773 2579
Fragments arranged by size:
2579 1773 1070 1020 845 620 409 157
Bpu10I CC'TnA_GC
Cuts at: 2799 3419 4998 5718 2799
Size: 620 1579 720 5554
Fragments arranged by size:
5554 1579 720 620
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3667 6598 7463 7761 3667
Size: 2931 865 298 4379
Fragments arranged by size:
4379 2931 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2271 4260 7136 882
Size: 617 772 1989 2876 2219
Fragments arranged by size:
2876 2219 1989 772 617
BsaAI yAC'GTr
Cuts at: 493 1204 3334 4503 4810 6264 493
Size: 711 2130 1169 307 1454 2702
Fragments arranged by size:
2702 2130 1454 1169 711 307
BsaBI GATnn'nnATC
Cuts at: 8467 8467
Size: 8473
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6280 6662 7297 275
Size: 53 83 186 936 4747 382 635 1451
Fragments arranged by size:
4747 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6906 7304 7578 7725 8136 6906
Size: 398 274 147 411 7243
Fragments arranged by size:
7243 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2268 2298 1178
Size: 30 165 30 865 30 7353
Fragments arranged by size:
7353 865 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2143 2158 2161 2164 2390 2804 4292 4411
Size: 957 15 3 3 226 414 1488 119
Cuts at: 4411 4592 5768 1186
Size: 181 1176 3891
Fragments arranged by size:
3891 1488 1176 957 414 226 181 119
15 3 3
BseYI C'CCAG_C
Cuts at: 2344 2496 2748 3506 3667 4660 5111 7676 8172
Size: 152 252 758 161 993 451 2565 496
Cuts at: 8172 2344
Size: 2645
Fragments arranged by size:
2645 2565 993 758 496 451 252 161
152
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3866 4906 1163
Size: 573 2130 1040 4730
Fragments arranged by size:
4730 2130 1040 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5923 6684 7712 1540
Size: 275 186 3922 761 1028 2301
Fragments arranged by size:
3922 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2985 3711 4284 4847 6534 6619 7690 2985
Size: 726 573 563 1687 85 1071 3768
Fragments arranged by size:
3768 1687 1071 726 573 563 85
BsmI GAATG_Cn'
Cuts at: 5984 6077 5984
Size: 93 8380
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2668 2985 3711 4284 4421 4542 4847
Size: 399 878 317 726 573 137 121 305
Cuts at: 4847 4944 5544 5589 5724 5748 5792 6534 6619
Size: 97 600 45 135 24 44 742 85
Cuts at: 6619 7690 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 878 742 726 600 573 399
317 305 137 135 121 97 85 45
44 24
BspEI T'CCGG_A
Cuts at: 8136 8136
Size: 8473
BspHI T'CATG_A
Cuts at: 3007 5656 6362 8092 3007
Size: 2649 706 1730 3388
Fragments arranged by size:
3388 2649 1730 706
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2549 3549 4652 5828 844
Size: 1705 1000 1103 1176 3489
Fragments arranged by size:
3489 1705 1176 1103 1000
BsrBI CCG'CTC
Cuts at: 1394 1823 4427 5149 6360 1394
Size: 429 2604 722 1211 3507
Fragments arranged by size:
3507 2604 1211 722 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2020 6968 7142 65
Size: 1183 772 4948 174 1396
Fragments arranged by size:
4948 1396 1183 772 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5706 7117 7304 1700
Size: 110 3896 1411 187 2869
Fragments arranged by size:
3896 2869 1411 187 110
BsrGI T'GTAC_A
Cuts at: 96 4667 96
Size: 4571 3902
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8473
BssSI C'ACGA_G
Cuts at: 2773 3751 6533 7545 2773
Size: 978 2782 1012 3701
Fragments arranged by size:
3701 2782 1012 978
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2593 794
Size: 1799 6674
BstBI TT'CG_AA
Cuts at: 7277 7277
Size: 8473
BstEII G'GTnAC_C
Cuts at: 1184 4639 1184
Size: 3455 5018
BstXI CCAn_nnnn'nTGG
Cuts at: 5626 5626
Size: 8473
BstYI r'GATC_y
Cuts at: 1972 2767 2964 3337 3367 3526 3730 3945 4686
Size: 795 197 373 30 159 204 215 741
Cuts at: 4686 5335 5415 5803 6170 6555 6572 8024 8110
Size: 649 80 388 367 385 17 1452 86
Cuts at: 8110 8122 8468 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 795 741 649 388 385 373
367 346 215 204 197 159 86 80
30 17 12
Bsu36I CC'TnA_GG
Cuts at: 3156 3156
Size: 8473
BtgI C'CryG_G
Cuts at: 513 1065 2763 3291 4159 513
Size: 552 1698 528 868 4827
Fragments arranged by size:
4827 1698 868 552 528
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2731 4901 487
Size: 683 56 1505 2170 4059
Fragments arranged by size:
4059 2170 1505 683 56
BtsI GCAGTG_nn'
Cuts at: 2127 3492 4304 4452 5029 5053 5080 5088 5124
Size: 1365 812 148 577 24 27 8 36
Cuts at: 5124 5317 5989 6783 6803 2127
Size: 193 672 794 20 3797
Fragments arranged by size:
3797 1365 812 794 672 577 193 148
36 27 24 20 8
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8473
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8438
DraI TTT'AAA
Cuts at: 5777 5777
Size: 8473
DraIII CAC_nnn'GTG
Cuts at: 3437 3437
Size: 8473
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7480 809
Size: 507 6164 1802
Fragments arranged by size:
6164 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2703 4405 5574
Size: 54 1425 50 275 18 873 1702 1169
Cuts at: 5574 5920 6809 8438 8
Size: 346 889 1629 43
Fragments arranged by size:
1702 1629 1425 1169 889 873 346 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5920 1537
Size: 275 4108 4090
Fragments arranged by size:
4108 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2161 2167 2182 2242 2319 2813 3776 4320
Size: 808 6 15 60 77 494 963 544
Cuts at: 4320 5724 6403 1353
Size: 1404 679 3423
Fragments arranged by size:
3423 1404 963 808 679 544 494 77
60 15 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3424 4433 7060 7434 7580 387
Size: 1064 138 1835 1009 2627 374 146 1280
Fragments arranged by size:
2627 1835 1280 1064 1009 374 146 138
EcoICRI GAG'CTC
Cuts at: 2983 4282 2983
Size: 1299 7174
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2259 2300 2927 3108 3127 3329 3719
Size: 393 627 41 627 181 19 202 390
Cuts at: 3719 4174 4540 4583 5203 5360 6536 7133 7920
Size: 455 366 43 620 157 1176 597 787
Cuts at: 7920 8188 1239
Size: 268 1524
Fragments arranged by size:
1524 1176 787 627 627 620 597 455
393 390 366 268 202 181 157 43
41 19
EcoNI CCTnn'n_nnAGG
Cuts at: 2789 4565 2789
Size: 1776 6697
EcoO109I rG'GnC_Cy
Cuts at: 2911 4570 4955 5116 2911
Size: 1659 385 161 6268
Fragments arranged by size:
6268 1659 385 161
EcoRI G'AATT_C
Cuts at: 3633 5782 6160 3633
Size: 2149 378 5946
Fragments arranged by size:
5946 2149 378
FspI TGC'GCA
Cuts at: 2366 6979 2366
Size: 4613 3860
FspAI rTGC'GCAy
Cuts at: 2366 2366
Size: 8473
HaeII r_GCGC'y
Cuts at: 1678 2521 5155 5197 7620 1678
Size: 843 2634 42 2423 2531
Fragments arranged by size:
2634 2531 2423 843 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2268 2300 2321 2353 3359
Size: 32 135 32 696 32 21 32 1006
Cuts at: 3359 3391 3592 3624 7114 7146 7188 7220 1373
Size: 32 201 32 3490 32 42 32 2626
Fragments arranged by size:
3490 2626 1006 696 201 135 42 32
32 32 32 32 32 32 32 21
HincII GTy'rAC
Cuts at: 669 1657 1938 4146 4341 5817 6063 669
Size: 988 281 2208 195 1476 246 3079
Fragments arranged by size:
3079 2208 1476 988 281 246 195
HindIII A'AGCT_T
Cuts at: 748 3839 748
Size: 3091 5382
HpaI GTT'AAC
Cuts at: 6063 6063
Size: 8473
KpnI G_GTAC'C
Cuts at: 2541 5798 2541
Size: 3257 5216
MfeI C'AATT_G
Cuts at: 3809 6072 3809
Size: 2263 6210
MluI A'CGCG_T
Cuts at: 8349 8349
Size: 8473
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3495 3719 3927 7587 7771 1305
Size: 2190 224 208 3660 184 2007
Fragments arranged by size:
3660 2190 2007 224 208 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4407 5576 10
Size: 54 1425 343 2575 1169 2907
Fragments arranged by size:
2907 2575 1425 1169 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3332 3678 5570 5582 5624 6431 6790
Size: 648 2166 346 1892 12 42 807 359
Cuts at: 6790 6949 518
Size: 159 2042
Fragments arranged by size:
2166 2042 1892 807 648 359 346 159
42 12
MspA1I CmG'CkG
Cuts at: 1606 2500 3418 4720 4829 5889 6566 7714 7959
Size: 894 918 1302 109 1060 677 1148 245
Cuts at: 7959 1606
Size: 2120
Fragments arranged by size:
2120 1302 1148 1060 918 894 677 245
109
NaeI GCC'GGC
Cuts at: 5708 5708
Size: 8473
NcoI C'CATG_G
Cuts at: 513 1065 3291 4159 513
Size: 552 2226 868 4827
Fragments arranged by size:
4827 2226 868 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8473
NgoMIV G'CCGG_C
Cuts at: 5706 5706
Size: 8473
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8473
NotI GC'GGCC_GC
Cuts at: 5920 5920
Size: 8473
NspI r_CATG'y
Cuts at: 1132 1229 3263 4258 5396 5449 5831 7376 1132
Size: 97 2034 995 1138 53 382 1545 2229
Fragments arranged by size:
2229 2034 1545 1138 995 382 97 53
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8473
PciI A'CATG_T
Cuts at: 7372 7372
Size: 8473
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4165 4414 1108
Size: 3057 249 5167
Fragments arranged by size:
5167 3057 249
PmeI GTTT'AAAC
Cuts at: 5777 5777
Size: 8473
PmlI CAC'GTG
Cuts at: 3334 4503 3334
Size: 1169 7304
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6542 6574 8055 8087 6542
Size: 32 1481 32 6928
Fragments arranged by size:
6928 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4570 4955 4570
Size: 385 8088
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8473
PsiI TTA'TAA
Cuts at: 6043 6043
Size: 8473
PspXI vC'TCGA_Gb
Cuts at: 2786 3719 5711 2786
Size: 933 1992 5548
Fragments arranged by size:
5548 1992 933
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2038 2070 2038
Size: 32 8441
PstI C_TGCA'G
Cuts at: 830 3936 4299 5159 5327 5825 830
Size: 3106 363 860 168 498 3478
Fragments arranged by size:
3478 3106 860 498 363 168
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8473
PvuII CAG'CTG
Cuts at: 2500 3418 4829 2500
Size: 918 1411 6144
Fragments arranged by size:
6144 1411 918
RsrII CG'GwC_CG
Cuts at: 5739 5739
Size: 8473
SacI G_AGCT'C
Cuts at: 2985 4284 2985
Size: 1299 7174
SalI G'TCGA_C
Cuts at: 1936 5815 1936
Size: 3879 4594
SbfI CC_TGCA'GG
Cuts at: 5825 5825
Size: 8473
ScaI AGT'ACT
Cuts at: 6721 8433 6721
Size: 1712 6761
SexAI A'CCwGG_T
Cuts at: 2280 2280
Size: 8473
SfcI C'TryA_G
Cuts at: 826 1045 2208 2812 3932 4134 4295 4485 5155
Size: 219 1163 604 1120 202 161 190 670
Cuts at: 5155 5323 5821 6956 7637 7828 826
Size: 168 498 1135 681 191 1471
Fragments arranged by size:
1471 1163 1135 1120 681 670 604 498
219 202 191 190 168 161
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8473
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8473
SmaI CCC'GGG
Cuts at: 1872 5800 1872
Size: 3928 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2058 2786 3646 3719 5711 6577
Size: 197 926 115 728 860 73 1992 866
Cuts at: 6577 7478 7740 820
Size: 901 262 1553
Fragments arranged by size:
1992 1553 926 901 866 860 728 262
197 115 73
SnaBI TAC'GTA
Cuts at: 493 6264 493
Size: 5771 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8473
SphI G_CATG'C
Cuts at: 1132 5449 5831 1132
Size: 4317 382 3774
Fragments arranged by size:
4317 3774 382
SspI AAT'ATT
Cuts at: 5 52 4906 6397 5
Size: 47 4854 1491 2081
Fragments arranged by size:
4854 2081 1491 47
StuI AGG'CCT
Cuts at: 1517 2877 3782 1517
Size: 1360 905 6208
Fragments arranged by size:
6208 1360 905
StyI C'CwwG_G
Cuts at: 513 1065 2736 3120 3291 3777 4159 4682 5912
Size: 552 1671 384 171 486 382 523 1230
Cuts at: 5912 513
Size: 3074
Fragments arranged by size:
3074 1671 1230 552 523 486 384 382
171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3344 4284 5181 5188 5756 6500 6517
Size: 1255 1764 940 897 7 568 744 17
Cuts at: 6517 6670 6855 7300 325
Size: 153 185 445 1498
Fragments arranged by size:
1764 1498 1255 940 897 744 568 445
185 153 17 7
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4667 6719 8431
Size: 274 80 33 51 1443 2690 2052 1712
Cuts at: 8431 96
Size: 138
Fragments arranged by size:
2690 2052 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2759 2791 3607 3639 4444 4476 4694 4726 8433
Size: 32 816 32 805 32 218 32 3707
Cuts at: 8433 8465 2759
Size: 32 2767
Fragments arranged by size:
3707 2767 816 805 218 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2282 1316
Size: 966 7507
XbaI T'CTAG_A
Cuts at: 5809 5809
Size: 8473
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2933 2933
Size: 8473
XhoI C'TCGA_G
Cuts at: 1943 2058 2786 3719 5711 1943
Size: 115 728 933 1992 4705
Fragments arranged by size:
4705 1992 933 728 115
XmaI C'CCGG_G
Cuts at: 1870 5798 1870
Size: 3928 4545
XmnI GAAnn'nnTTC
Cuts at: 6602 6602
Size: 8473
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6281 276
Size: 53 83 186 936 4747 2468
Fragments arranged by size:
4747 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PstI PvuI PvuII RsrII SacI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsiWI BsmBI BstZ17I EcoRV FalI FseI KasI
NarI NruI NsiI PacI PfoI PspOMI SacII SanDI
SapI SfiI SfoI SrfI SwaI