Restriction map for
pFN21AA0930
(Circular) (Six-base) MAPSORT of: pFN21AA0930.seq Check: 4433 from: 1 to: 5944
pFN21AA0930 5944 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3754 278
Size: 53 83 186 936 2218 2468
Fragments arranged by size:
2468 2218 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2816 3287 1937
Size: 879 471 4594
Fragments arranged by size:
4594 879 471
Acc65I G'GTAC_C
Cuts at: 3265 3265
Size: 5944
AclI AA'CG_TT
Cuts at: 1563 4071 4444 1563
Size: 2508 373 3063
Fragments arranged by size:
3063 2508 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2865 2878 3143 4007 5391 5659 1632
Size: 1233 13 265 864 1384 268 1917
Fragments arranged by size:
1917 1384 1233 864 268 265 13
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5747
AflIII A'CryG_T
Cuts at: 1203 2399 2619 4843 5820 1203
Size: 1196 220 2224 977 1327
Fragments arranged by size:
2224 1327 1196 977 220
AgeI A'CCGG_T
Cuts at: 4775 4775
Size: 5944
AhdI GACnn_n'nnGTC
Cuts at: 4673 4673
Size: 5944
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5944
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5944
AlwNI CAG_nnn'CTG
Cuts at: 2368 3687 5259 2368
Size: 1319 1572 3053
Fragments arranged by size:
3053 1572 1319
ApaI G_GGCC'C
Cuts at: 2774 2774
Size: 5944
ApaLI G'TGCA_C
Cuts at: 2395 4001 5157 2395
Size: 1606 1156 3182
Fragments arranged by size:
3182 1606 1156
ApoI r'AATT_y
Cuts at: 1493 3253 3479 3631 5740 1493
Size: 1760 226 152 2109 1697
Fragments arranged by size:
2109 1760 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4498 160
Size: 872 3466 1606
Fragments arranged by size:
3466 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5944
AvaI C'yCGr_G
Cuts at: 1870 1943 3269 1870
Size: 73 1326 4545
Fragments arranged by size:
4545 1326 73
BamHI G'GATC_C
Cuts at: 3274 3641 3274
Size: 367 5577
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2962 3265 4720 618
Size: 325 217 627 1175 303 1455 1842
Fragments arranged by size:
1842 1455 1175 627 325 303 217
BanII G_rGCy'C
Cuts at: 1391 2167 2648 2774 3263 1391
Size: 776 481 126 489 4072
Fragments arranged by size:
4072 776 489 481 126
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2113 928
Size: 971 214 4759
Fragments arranged by size:
4759 971 214
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2213 2247 4135 4169 1903
Size: 34 276 34 1888 34 3678
Fragments arranged by size:
3678 1888 276 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3836 5046 3836
Size: 1210 4734
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5944
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2056 2708 2778
Size: 107 122 71 631 605 384 652 70
Cuts at: 2778 2798 4555 136
Size: 20 1757 1525
Fragments arranged by size:
1757 1525 652 631 605 384 122 107
71 70 20
BglII A'GATC_T
Cuts at: 5939 5939
Size: 5944
BlpI GC'TnA_GC
Cuts at: 2253 3361 2253
Size: 1108 4836
Bme1580I G_kGCm'C
Cuts at: 1790 2399 2774 2967 3095 4005 5161 1790
Size: 609 375 193 128 910 1156 2573
Fragments arranged by size:
2573 1156 910 609 375 193 128
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5944
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2338 2457 3675
Size: 416 53 83 399 24 921 119 1218
Cuts at: 3675 4633 442
Size: 958 1753
Fragments arranged by size:
1753 1218 958 921 416 399 119 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5944
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2839 4604 1239
Size: 1600 1765 2579
Fragments arranged by size:
2579 1765 1600
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2651 2936 4069 4934 5232 2651
Size: 285 1133 865 298 3363
Fragments arranged by size:
3363 1133 865 298 285
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2307 3081 4607 882
Size: 617 808 774 1526 2219
Fragments arranged by size:
2219 1526 808 774 617
BsaAI yAC'GTr
Cuts at: 493 1204 2402 3735 493
Size: 711 1198 1333 2702
Fragments arranged by size:
2702 1333 1198 711
BsaBI GATnn'nnATC
Cuts at: 5938 5938
Size: 5944
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3751 4133 4768 275
Size: 53 83 186 936 2218 382 635 1451
Fragments arranged by size:
2218 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2973 4377 4775 5049 5196 5607 2973
Size: 1404 398 274 147 411 3310
Fragments arranged by size:
3310 1404 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2568 2598 1178
Size: 30 165 30 1165 30 4524
Fragments arranged by size:
4524 1165 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2348 2507 2558 3032 1186
Size: 1162 159 51 474 4098
Fragments arranged by size:
4098 1162 474 159 51
BseYI C'CCAG_C
Cuts at: 2860 5147 5643 2860
Size: 2287 496 3161
Fragments arranged by size:
3161 2287 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2253 3038 1163
Size: 573 517 785 4069
Fragments arranged by size:
4069 785 573 517
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3394 4155 5183 1540
Size: 275 186 1393 761 1028 2301
Fragments arranged by size:
2301 1393 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2399 4005 4090 5161 2399
Size: 1606 85 1071 3182
Fragments arranged by size:
3182 1606 1071 85
BsmI GAATG_Cn'
Cuts at: 3455 3548 3455
Size: 93 5851
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2167 2399 2648 2774 2967 3095 3263
Size: 399 377 232 249 126 193 128 168
Cuts at: 3263 4005 4090 5161 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 377 249 232 193
168 128 126 85
BspEI T'CCGG_A
Cuts at: 5607 5607
Size: 5944
BspHI T'CATG_A
Cuts at: 2526 3833 5563 2526
Size: 1307 1730 2907
Fragments arranged by size:
2907 1730 1307
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3078 3299 844
Size: 2234 221 3489
Fragments arranged by size:
3489 2234 221
BsrBI CCG'CTC
Cuts at: 1394 1823 2881 3831 1394
Size: 429 1058 950 3507
Fragments arranged by size:
3507 1058 950 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4439 4613 65
Size: 1183 3191 174 1396
Fragments arranged by size:
3191 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2280 2925 4588 4775 1700
Size: 110 470 645 1663 187 2869
Fragments arranged by size:
2869 1663 645 470 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5944
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5944
BssSI C'ACGA_G
Cuts at: 2876 4004 5016 2876
Size: 1128 1012 3804
Fragments arranged by size:
3804 1128 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5944
BstBI TT'CG_AA
Cuts at: 4748 4748
Size: 5944
BstEII G'GTnAC_C
Cuts at: 1184 2380 2884 1184
Size: 1196 504 4244
Fragments arranged by size:
4244 1196 504
BstYI r'GATC_y
Cuts at: 1972 3274 3641 4026 4043 5495 5581 5593 5939
Size: 1302 367 385 17 1452 86 12 346
Cuts at: 5939 1972
Size: 1977
Fragments arranged by size:
1977 1452 1302 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2259 2259
Size: 5944
BtgI C'CryG_G
Cuts at: 513 1065 2475 513
Size: 552 1410 3982
Fragments arranged by size:
3982 1410 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5205
Fragments arranged by size:
5205 683 56
BtsI GCAGTG_nn'
Cuts at: 3460 4254 4274 3460
Size: 794 20 5130
Fragments arranged by size:
5130 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5944
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5909
DraI TTT'AAA
Cuts at: 3248 3248
Size: 5944
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2117 4951 809
Size: 507 801 2834 1802
Fragments arranged by size:
2834 1802 801 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2057 2697
Size: 54 1425 50 275 18 198 29 640
Cuts at: 2697 3003 3391 4280 5909 8
Size: 306 388 889 1629 43
Fragments arranged by size:
1629 1425 889 640 388 306 275 198
54 50 43 29 18
EagI C'GGCC_G
Cuts at: 1537 1812 3391 1537
Size: 275 1579 4090
Fragments arranged by size:
4090 1579 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2841 3874 1353
Size: 1488 1033 3423
Fragments arranged by size:
3423 1488 1033
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4531 4905 5051 387
Size: 1064 138 2942 374 146 1280
Fragments arranged by size:
2942 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2839 2865 2878 3143 4007 4604 5391
Size: 393 1207 26 13 265 864 597 787
Cuts at: 5391 5659 1239
Size: 268 1524
Fragments arranged by size:
1524 1207 864 787 597 393 268 265
26 13
EcoO109I rG'GnC_Cy
Cuts at: 2770 2771 3114 2770
Size: 1 343 5600
Fragments arranged by size:
5600 343 1
EcoRI G'AATT_C
Cuts at: 3253 3631 3253
Size: 378 5566
FspI TGC'GCA
Cuts at: 2762 4450 2762
Size: 1688 4256
FspAI rTGC'GCAy
Cuts at: 2762 2762
Size: 5944
HaeII r_GCGC'y
Cuts at: 1678 2666 5091 1678
Size: 988 2425 2531
Fragments arranged by size:
2531 2425 988
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2568 2600 4585 4617 4659
Size: 32 135 32 996 32 1985 32 42
Cuts at: 4659 4691 1373
Size: 32 2626
Fragments arranged by size:
2626 1985 996 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3288 3534 669
Size: 988 281 1350 246 3079
Fragments arranged by size:
3079 1350 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5944
HpaI GTT'AAC
Cuts at: 3534 3534
Size: 5944
KpnI G_GTAC'C
Cuts at: 3269 3269
Size: 5944
MfeI C'AATT_G
Cuts at: 3543 3543
Size: 5944
MluI A'CGCG_T
Cuts at: 5820 5820
Size: 5944
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5058 5242 1305
Size: 3753 184 2007
Fragments arranged by size:
3753 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2030 10
Size: 54 1425 343 198 3924
Fragments arranged by size:
3924 1425 343 198 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3236 3902 4261 4420 518
Size: 648 2070 666 359 159 2042
Fragments arranged by size:
2070 2042 666 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2252 3187 3360 4037 5185 5430 1606
Size: 646 935 173 677 1148 245 2120
Fragments arranged by size:
2120 1148 935 677 646 245 173
NcoI C'CATG_G
Cuts at: 513 1065 2475 513
Size: 552 1410 3982
Fragments arranged by size:
3982 1410 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5944
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5944
NotI GC'GGCC_GC
Cuts at: 3391 3391
Size: 5944
NspI r_CATG'y
Cuts at: 1132 1229 2185 3302 4847 1132
Size: 97 956 1117 1545 2229
Fragments arranged by size:
2229 1545 1117 956 97
PasI CC'CwG_GG
Cuts at: 1348 2776 1348
Size: 1428 4516
PciI A'CATG_T
Cuts at: 4843 4843
Size: 5944
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5944
PfoI T'CCnGG_A
Cuts at: 3027 3210 3027
Size: 183 5761
PmeI GTTT'AAAC
Cuts at: 3248 3248
Size: 5944
PmlI CAC'GTG
Cuts at: 2402 2402
Size: 5944
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2534 2566 4013 4045 5526 5558 2534
Size: 32 1447 32 1481 32 2920
Fragments arranged by size:
2920 1481 1447 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3114 3114
Size: 5944
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5944
PsiI TTA'TAA
Cuts at: 3514 3514
Size: 5944
PspOMI G'GGCC_C
Cuts at: 2770 2770
Size: 5944
PstI C_TGCA'G
Cuts at: 830 2095 2364 3296 830
Size: 1265 269 932 3478
Fragments arranged by size:
3478 1265 932 269
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5944
PvuII CAG'CTG
Cuts at: 2252 2252
Size: 5944
SalI G'TCGA_C
Cuts at: 1936 3286 1936
Size: 1350 4594
SbfI CC_TGCA'GG
Cuts at: 3296 3296
Size: 5944
ScaI AGT'ACT
Cuts at: 4192 5904 4192
Size: 1712 4232
SfcI C'TryA_G
Cuts at: 826 1045 2091 2360 2817 3292 4427 5108 5299
Size: 219 1046 269 457 475 1135 681 191
Cuts at: 5299 826
Size: 1471
Fragments arranged by size:
1471 1135 1046 681 475 457 269 219
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2056 2056
Size: 5944
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5944
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5944
SmaI CCC'GGG
Cuts at: 1872 3271 1872
Size: 1399 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2666 2951 4048 4949 5211 820
Size: 197 926 723 285 1097 901 262 1553
Fragments arranged by size:
1553 1097 926 901 723 285 262 197
SnaBI TAC'GTA
Cuts at: 493 3735 493
Size: 3242 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5944
SphI G_CATG'C
Cuts at: 1132 3302 1132
Size: 2170 3774
SspI AAT'ATT
Cuts at: 5 52 3868 5
Size: 47 3816 2081
Fragments arranged by size:
3816 2081 47
StuI AGG'CCT
Cuts at: 1517 2307 1517
Size: 790 5154
StyI C'CwwG_G
Cuts at: 513 1065 2031 2475 3383 513
Size: 552 966 444 908 3074
Fragments arranged by size:
3074 966 908 552 444
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3069 3971 3988 4141 4326 4771 325
Size: 1255 1489 902 17 153 185 445 1498
Fragments arranged by size:
1498 1489 1255 902 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2739 4190 5902
Size: 274 80 33 51 1443 762 1451 1712
Cuts at: 5902 96
Size: 138
Fragments arranged by size:
1712 1451 1443 762 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5904 5936 5904
Size: 32 5912
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5944
XbaI T'CTAG_A
Cuts at: 3280 3280
Size: 5944
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2149 2149
Size: 5944
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5944
XmaI C'CCGG_G
Cuts at: 1870 3269 1870
Size: 1399 4545
XmnI GAAnn'nnTTC
Cuts at: 4073 4073
Size: 5944
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3752 276
Size: 53 83 186 936 2218 2468
Fragments arranged by size:
2468 2218 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoRV FalI FseI KasI NaeI NarI
NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfoI SrfI SwaI