Restriction map for
pFN21AA0926
(Circular) (Six-base) MAPSORT of: pFN21AA0926.seq Check: 4604 from: 1 to: 8992
pFN21AA0926 8992 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3031 3031
Size: 8992
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6802 278
Size: 53 83 186 936 5266 2468
Fragments arranged by size:
5266 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3658 6335 1937
Size: 1721 2677 4594
Fragments arranged by size:
4594 2677 1721
Acc65I G'GTAC_C
Cuts at: 6313 6313
Size: 8992
AclI AA'CG_TT
Cuts at: 1563 4190 7119 7492 1563
Size: 2627 2929 373 3063
Fragments arranged by size:
3063 2929 2627 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2072 2087 3088 4289 4454 4796 4939 6236
Size: 440 15 1001 1201 165 342 143 1297
Cuts at: 6236 7055 8439 8707 1632
Size: 819 1384 268 1917
Fragments arranged by size:
1917 1384 1297 1201 1001 819 440 342
268 165 143 15
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8795
AflIII A'CryG_T
Cuts at: 1203 4016 7891 8868 1203
Size: 2813 3875 977 1327
Fragments arranged by size:
3875 2813 1327 977
AgeI A'CCGG_T
Cuts at: 5161 7823 5161
Size: 2662 6330
AhdI GACnn_n'nnGTC
Cuts at: 2503 7721 2503
Size: 5218 3774
AleI CACnn'nnGTG
Cuts at: 1166 2348 2546 5702 1166
Size: 1182 198 3156 4456
Fragments arranged by size:
4456 3156 1182 198
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3024 1184
Size: 1840 7152
AlwNI CAG_nnn'CTG
Cuts at: 2083 3199 4132 4603 4669 4699 4744 4915 5410
Size: 1116 933 471 66 30 45 171 495
Cuts at: 5410 6007 6094 6735 8307 2083
Size: 597 87 641 1572 2768
Fragments arranged by size:
2768 1572 1116 933 641 597 495 471
171 87 66 45 30
ApaI G_GGCC'C
Cuts at: 3550 4924 5249 3550
Size: 1374 325 7293
Fragments arranged by size:
7293 1374 325
ApaLI G'TGCA_C
Cuts at: 2386 4264 5495 7049 8205 2386
Size: 1878 1231 1554 1156 3173
Fragments arranged by size:
3173 1878 1554 1231 1156
ApoI r'AATT_y
Cuts at: 1493 2643 2834 4280 5387 6301 6527 6679 8788
Size: 1150 191 1446 1107 914 226 152 2109
Cuts at: 8788 1493
Size: 1697
Fragments arranged by size:
2109 1697 1446 1150 1107 914 226 191
152
AseI AT'TA_AT
Cuts at: 160 1032 7546 160
Size: 872 6514 1606
Fragments arranged by size:
6514 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8992
AvaI C'yCGr_G
Cuts at: 1870 1943 3353 6045 6317 1870
Size: 73 1410 2692 272 4545
Fragments arranged by size:
4545 2692 1410 272 73
BamHI G'GATC_C
Cuts at: 2364 2564 6322 6689 2364
Size: 200 3758 367 4667
Fragments arranged by size:
4667 3758 367 200
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 6313 7768 618
Size: 325 217 627 4526 1455 1842
Fragments arranged by size:
4526 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2114 2399 3153 3282 3543 3550 4257 4346
Size: 723 285 754 129 261 7 707 89
Cuts at: 4346 4842 4924 5249 5799 6311 1391
Size: 496 82 325 550 512 4072
Fragments arranged by size:
4072 754 723 707 550 512 496 325
285 261 129 89 82 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2150 3088 5826 928
Size: 971 251 938 2738 4094
Fragments arranged by size:
4094 2738 971 938 251
BbvCI CC'TCA_GC
Cuts at: 2240 2574 3732 4557 4941 2240
Size: 334 1158 825 384 6291
Fragments arranged by size:
6291 1158 825 384 334
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7183 7217 1903
Size: 34 5246 34 3678
Fragments arranged by size:
5246 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2965 4993 6884 8094 2965
Size: 2028 1891 1210 3863
Fragments arranged by size:
3863 2028 1891 1210
BclI T'GATC_A
Cuts at: 1548 3369 1548
Size: 1821 7171
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3179 3214 7603
Size: 107 122 71 631 605 1507 35 4389
Cuts at: 7603 136
Size: 1525
Fragments arranged by size:
4389 1525 1507 631 605 122 107 71
35
BglII A'GATC_T
Cuts at: 5112 5932 8987 5112
Size: 820 3055 5117
Fragments arranged by size:
5117 3055 820
BlpI GC'TnA_GC
Cuts at: 2133 3147 3687 4463 5472 6095 6409 2133
Size: 1014 540 776 1009 623 314 4716
Fragments arranged by size:
4716 1014 1009 776 623 540 314
Bme1580I G_kGCm'C
Cuts at: 1790 2390 3550 3564 4268 4924 5249 5499 7053
Size: 600 1160 14 704 656 325 250 1554
Cuts at: 7053 8209 1790
Size: 1156 2573
Fragments arranged by size:
2573 1554 1160 1156 704 656 600 325
250 14
BmgBI CAC'GTC
Cuts at: 1318 4725 5104 1318
Size: 3407 379 5206
Fragments arranged by size:
5206 3407 379
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3118 4370 6723
Size: 416 53 83 399 24 1701 1252 2353
Cuts at: 6723 7681 442
Size: 958 1753
Fragments arranged by size:
2353 1753 1701 1252 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8992
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2182 2807 4524 5161 2182
Size: 625 1717 637 6013
Fragments arranged by size:
6013 1717 637 625
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2089 2467 3313 4172 4541 4665 4946 5499
Size: 850 378 846 859 369 124 281 553
Cuts at: 5499 5606 5813 5835 5946 7652 1239
Size: 107 207 22 111 1706 2579
Fragments arranged by size:
2579 1706 859 850 846 553 378 369
281 207 124 111 107 22
Bpu10I CC'TnA_GC
Cuts at: 2240 2574 3732 4557 4619 4841 4941 5079 2240
Size: 334 1158 825 62 222 100 138 6153
Fragments arranged by size:
6153 1158 825 334 222 138 100 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4400 5018 5181 6187 7117 7982 8280 4400
Size: 618 163 1006 930 865 298 5112
Fragments arranged by size:
5112 1006 930 865 618 298 163
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2328 3705 4517 5351 6215 7655 882
Size: 617 829 1377 812 834 864 1440 2219
Fragments arranged by size:
2219 1440 1377 864 834 829 812 617
BsaAI yAC'GTr
Cuts at: 493 1204 4294 6783 493
Size: 711 3090 2489 2702
Fragments arranged by size:
3090 2702 2489 711
BsaBI GATnn'nnATC
Cuts at: 8986 8986
Size: 8992
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2440 4504 6799 7181
Size: 53 83 186 936 907 2064 2295 382
Cuts at: 7181 7816 275
Size: 635 1451
Fragments arranged by size:
2295 2064 1451 936 907 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5161 6183 7425 7823 8097 8244 8655 5161
Size: 1022 1242 398 274 147 411 5498
Fragments arranged by size:
5498 1242 1022 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3160 3166 3190 3196 1178
Size: 30 165 30 1757 6 24 6 6974
Fragments arranged by size:
6974 1757 165 30 30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2075 2459 2933 5585 1186
Size: 889 384 474 2652 4593
Fragments arranged by size:
4593 2652 889 474 384
BseYI C'CCAG_C
Cuts at: 2327 2551 2559 2579 3322 3334 3702 4769 5633
Size: 224 8 20 743 12 368 1067 864
Cuts at: 5633 6140 8195 8691 2327
Size: 507 2055 496 2628
Fragments arranged by size:
2628 2055 1067 864 743 507 496 368
224 20 12 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3272 3568 5993 1163
Size: 573 1536 296 2425 4162
Fragments arranged by size:
4162 2425 1536 573 296
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6442 7203 8231 1540
Size: 275 186 4441 761 1028 2301
Fragments arranged by size:
4441 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2114 2390 3153 3282 3543 3843 4257 4268 4635
Size: 276 763 129 261 300 414 11 367
Cuts at: 4635 5499 5799 7053 7138 8209 2114
Size: 864 300 1254 85 1071 2897
Fragments arranged by size:
2897 1254 1071 864 763 414 367 300
300 276 261 129 85 11
BsmI GAATG_Cn'
Cuts at: 6503 6596 6503
Size: 93 8899
BsmBI CGTCTCn'nnnn_
Cuts at: 4495 4495
Size: 8992
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2114 2390 2399 3153 3282 3543 3550
Size: 399 324 276 9 754 129 261 7
Cuts at: 3550 3564 3843 4257 4268 4346 4635 4842 4924
Size: 14 279 414 11 78 289 207 82
Cuts at: 4924 5249 5499 5799 6311 7053 7138 8209 1391
Size: 325 250 300 512 742 85 1071 2174
Fragments arranged by size:
2174 1071 754 742 512 414 399 325
324 300 289 279 276 261 250 207
129 85 82 78 14 11 9 7
BspEI T'CCGG_A
Cuts at: 8655 8655
Size: 8992
BspHI T'CATG_A
Cuts at: 6881 8611 6881
Size: 1730 7262
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3031 4797 5214 6347 844
Size: 2187 1766 417 1133 3489
Fragments arranged by size:
3489 2187 1766 1133 417
BsrBI CCG'CTC
Cuts at: 1394 1823 2132 3214 6879 1394
Size: 429 309 1082 3665 3507
Fragments arranged by size:
3665 3507 1082 429 309
BsrDI GCAATG_nn'
Cuts at: 65 1248 3906 7487 7661 65
Size: 1183 2658 3581 174 1396
Fragments arranged by size:
3581 2658 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2377 3307 4080 5161 7636 7823 1700
Size: 110 567 930 773 1081 2475 187 2869
Fragments arranged by size:
2869 2475 1081 930 773 567 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8992
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8992
BssSI C'ACGA_G
Cuts at: 6133 7052 8064 6133
Size: 919 1012 7061
Fragments arranged by size:
7061 1012 919
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4132 4272 6007 794
Size: 3338 140 1735 3779
Fragments arranged by size:
3779 3338 1735 140
BstBI TT'CG_AA
Cuts at: 4218 7796 4218
Size: 3578 5414
BstEII G'GTnAC_C
Cuts at: 1184 4420 5371 1184
Size: 3236 951 4805
Fragments arranged by size:
4805 3236 951
BstXI CCAn_nnnn'nTGG
Cuts at: 3074 3074
Size: 8992
BstYI r'GATC_y
Cuts at: 1972 2364 2564 2881 3195 5112 5932 6322 6689
Size: 392 200 317 314 1917 820 390 367
Cuts at: 6689 7074 7091 8543 8629 8641 8987 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1917 1452 820 392 390 385 367
346 317 314 200 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3776 5733 3776
Size: 1957 7035
BtgI C'CryG_G
Cuts at: 513 1065 2004 2702 2765 4350 5250 513
Size: 552 939 698 63 1585 900 4255
Fragments arranged by size:
4255 1585 939 900 698 552 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8253
Fragments arranged by size:
8253 683 56
BtsI GCAGTG_nn'
Cuts at: 2355 2553 3317 4075 4115 4162 4482 6508 7302
Size: 198 764 758 40 47 320 2026 794
Cuts at: 7302 7322 2355
Size: 20 4025
Fragments arranged by size:
4025 2026 794 764 758 320 198 47
40 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8992
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8957
DraI TTT'AAA
Cuts at: 5566 6296 5566
Size: 730 8262
DraIII CAC_nnn'GTG
Cuts at: 5512 6016 5512
Size: 504 8488
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7999 809
Size: 507 6683 1802
Fragments arranged by size:
6683 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3033 5848 6439
Size: 54 1425 50 275 18 1203 2815 591
Cuts at: 6439 7328 8957 8
Size: 889 1629 43
Fragments arranged by size:
2815 1629 1425 1203 889 591 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6439 1537
Size: 275 4627 4090
Fragments arranged by size:
4627 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2119 2487 3227 4409 4475 6922 1353
Size: 766 368 740 1182 66 2447 3423
Fragments arranged by size:
3423 2447 1182 766 740 368 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2028 3326 7579 7953 8099 387
Size: 1064 138 439 1298 4253 374 146 1280
Fragments arranged by size:
4253 1298 1280 1064 439 374 146 138
EcoICRI GAG'CTC
Cuts at: 2112 3151 3280 3541 4255 5797 2112
Size: 1039 129 261 714 1542 5307
Fragments arranged by size:
5307 1542 1039 714 261 129
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2087 2089 2467 3088 3313 4172
Size: 393 440 15 2 378 621 225 859
Cuts at: 4172 4289 4454 4541 4665 4796 4939 4946 5499
Size: 117 165 87 124 131 143 7 553
Cuts at: 5499 5606 5813 5835 5946 6236 7055 7652 8439
Size: 107 207 22 111 290 819 597 787
Cuts at: 8439 8707 1239
Size: 268 1524
Fragments arranged by size:
1524 859 819 787 621 597 553 440
393 378 290 268 225 207 165 143
131 124 117 111 107 87 22 15
7 2
EcoNI CCTnn'n_nnAGG
Cuts at: 2054 3671 4928 2054
Size: 1617 1257 6118
Fragments arranged by size:
6118 1617 1257
EcoO109I rG'GnC_Cy
Cuts at: 2206 2613 2697 3436 3547 3698 4114 4372 4570
Size: 407 84 739 111 151 416 258 198
Cuts at: 4570 4741 4920 4921 4993 5203 5245 5246 5255
Size: 171 179 1 72 210 42 1 9
Cuts at: 5255 5450 2206
Size: 195 5748
Fragments arranged by size:
5748 739 416 407 258 210 198 195
179 171 151 111 84 72 42 9
1 1
EcoRI G'AATT_C
Cuts at: 5387 6301 6679 5387
Size: 914 378 7700
Fragments arranged by size:
7700 914 378
FspI TGC'GCA
Cuts at: 7498 7498
Size: 8992
HaeII r_GCGC'y
Cuts at: 1678 8139 1678
Size: 6461 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2116 2148 2489 2521 2621
Size: 32 135 32 544 32 341 32 100
Cuts at: 2621 2653 3160 3192 3361 3373 3393 3405 3690
Size: 32 507 32 169 12 20 12 285
Cuts at: 3690 3704 3722 3736 4733 4765 5850 5882 6214
Size: 14 18 14 997 32 1085 32 332
Cuts at: 6214 6246 7633 7665 7707 7739 1373
Size: 32 1387 32 42 32 2626
Fragments arranged by size:
2626 1387 1085 997 544 507 341 332
285 169 135 100 42 32 32 32
32 32 32 32 32 32 32 32
20 18 14 14 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3027 5278 6336 6582 669
Size: 988 281 1089 2251 1058 246 3079
Fragments arranged by size:
3079 2251 1089 1058 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4301 748
Size: 3553 5439
HpaI GTT'AAC
Cuts at: 6582 6582
Size: 8992
KpnI G_GTAC'C
Cuts at: 6317 6317
Size: 8992
MfeI C'AATT_G
Cuts at: 5121 6591 5121
Size: 1470 7522
MluI A'CGCG_T
Cuts at: 8868 8868
Size: 8992
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 8106 8290 1305
Size: 6801 184 2007
Fragments arranged by size:
6801 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3035 5850 10
Size: 54 1425 343 1203 2815 3152
Fragments arranged by size:
3152 2815 1425 1203 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2159 2348 2546 5702 6950 7309 7468
Size: 648 993 189 198 3156 1248 359 159
Cuts at: 7468 518
Size: 2042
Fragments arranged by size:
3156 2042 1248 993 648 359 198 189
159
MspA1I CmG'CkG
Cuts at: 1606 2197 2422 2445 2711 2845 3070 3108 4129
Size: 591 225 23 266 134 225 38 1021
Cuts at: 4129 4309 4556 4705 4713 4945 5331 5436 5827
Size: 180 247 149 8 232 386 105 391
Cuts at: 5827 6037 6408 7085 8233 8478 1606
Size: 210 371 677 1148 245 2120
Fragments arranged by size:
2120 1148 1021 677 591 391 386 371
266 247 245 232 225 225 210 180
149 134 105 38 23 8
NaeI GCC'GGC
Cuts at: 2379 3309 4082 2379
Size: 930 773 7289
Fragments arranged by size:
7289 930 773
NcoI C'CATG_G
Cuts at: 513 1065 2004 2702 2765 4350 513
Size: 552 939 698 63 1585 5155
Fragments arranged by size:
5155 1585 939 698 552 63
NdeI CA'TA_TG
Cuts at: 387 3982 387
Size: 3595 5397
NgoMIV G'CCGG_C
Cuts at: 2377 3307 4080 2377
Size: 930 773 7289
Fragments arranged by size:
7289 930 773
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8992
NotI GC'GGCC_GC
Cuts at: 6439 6439
Size: 8992
NspI r_CATG'y
Cuts at: 1132 1229 3095 4234 6350 7895 1132
Size: 97 1866 1139 2116 1545 2229
Fragments arranged by size:
2229 2116 1866 1545 1139 97
PasI CC'CwG_GG
Cuts at: 1348 2392 3564 1348
Size: 1044 1172 6776
Fragments arranged by size:
6776 1172 1044
PciI A'CATG_T
Cuts at: 7891 7891
Size: 8992
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4744 5410 1108
Size: 3636 666 4690
Fragments arranged by size:
4690 3636 666
PfoI T'CCnGG_A
Cuts at: 2105 4518 5097 5583 2105
Size: 2413 579 486 5514
Fragments arranged by size:
5514 2413 579 486
PmeI GTTT'AAAC
Cuts at: 6296 6296
Size: 8992
PmlI CAC'GTG
Cuts at: 4294 4294
Size: 8992
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2779 2811 5781 5813 7061 7093 8574 8606 2779
Size: 32 2970 32 1248 32 1481 32 3165
Fragments arranged by size:
3165 2970 1481 1248 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2206 2613 3436 3698 4114 4372 4570 4741 5203
Size: 407 823 262 416 258 198 171 462
Cuts at: 5203 2206
Size: 5995
Fragments arranged by size:
5995 823 462 416 407 262 258 198
171
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8992
PsiI TTA'TAA
Cuts at: 6562 6562
Size: 8992
PspOMI G'GGCC_C
Cuts at: 3546 4920 5245 3546
Size: 1374 325 7293
Fragments arranged by size:
7293 1374 325
PstI C_TGCA'G
Cuts at: 830 3003 3113 3396 4128 4140 4477 4704 4794
Size: 2173 110 283 732 12 337 227 90
Cuts at: 4794 6344 830
Size: 1550 3478
Fragments arranged by size:
3478 2173 1550 732 337 283 227 110
90 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8992
PvuII CAG'CTG
Cuts at: 2422 2845 3070 3108 4129 4309 4556 4705 4713
Size: 423 225 38 1021 180 247 149 8
Cuts at: 4713 4945 5436 5827 6037 2422
Size: 232 491 391 210 5377
Fragments arranged by size:
5377 1021 491 423 391 247 232 225
210 180 149 38 8
SacI G_AGCT'C
Cuts at: 2114 3153 3282 3543 4257 5799 2114
Size: 1039 129 261 714 1542 5307
Fragments arranged by size:
5307 1542 1039 714 261 129
SalI G'TCGA_C
Cuts at: 1936 6334 1936
Size: 4398 4594
SanDI GG'GwC_CC
Cuts at: 2613 3698 4114 4372 2613
Size: 1085 416 258 7233
Fragments arranged by size:
7233 1085 416 258
SapI GCTCTTCn'nnn_
Cuts at: 2119 2487 3227 2119
Size: 368 740 7884
Fragments arranged by size:
7884 740 368
SbfI CC_TGCA'GG
Cuts at: 6344 6344
Size: 8992
ScaI AGT'ACT
Cuts at: 7240 8952 7240
Size: 1712 7280
SexAI A'CCwGG_T
Cuts at: 2172 2172
Size: 8992
SfcI C'TryA_G
Cuts at: 826 1045 2296 2747 2999 3109 3392 3475 3859
Size: 219 1251 451 252 110 283 83 384
Cuts at: 3859 4124 4136 4473 4691 4700 4790 5313 6340
Size: 265 12 337 218 9 90 523 1027
Cuts at: 6340 7475 8156 8347 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1251 1135 1027 681 523 451 384
337 283 265 252 219 218 191 110
90 83 12 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8992
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8992
SmaI CCC'GGG
Cuts at: 1872 6319 1872
Size: 4447 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4415 5033 5196 6166 7096 7997
Size: 197 926 2472 618 163 970 930 901
Cuts at: 7997 8259 820
Size: 262 1553
Fragments arranged by size:
2472 1553 970 930 926 901 618 262
197 163
SnaBI TAC'GTA
Cuts at: 493 6783 493
Size: 6290 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8992
SphI G_CATG'C
Cuts at: 1132 6350 1132
Size: 5218 3774
SspI AAT'ATT
Cuts at: 5 52 3470 6916 5
Size: 47 3418 3446 2081
Fragments arranged by size:
3446 3418 2081 47
StuI AGG'CCT
Cuts at: 1517 2967 5915 1517
Size: 1450 2948 4594
Fragments arranged by size:
4594 2948 1450
StyI C'CwwG_G
Cuts at: 513 1065 2004 2702 2765 3129 4350 5521 5646
Size: 552 939 698 63 364 1221 1171 125
Cuts at: 5646 5916 6431 513
Size: 270 515 3074
Fragments arranged by size:
3074 1221 1171 939 698 552 515 364
270 125 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2405 2539 2605 2778 3556 4622 5402
Size: 1255 825 134 66 173 778 1066 780
Cuts at: 5402 7019 7036 7189 7374 7819 325
Size: 1617 17 153 185 445 1498
Fragments arranged by size:
1617 1498 1255 1066 825 780 778 445
185 173 153 134 66 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 7238 8950 96
Size: 274 80 33 51 1443 5261 1712 138
Fragments arranged by size:
5261 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3166 3198 4957 4989 5159 5191 6173 6205 8952
Size: 32 1759 32 170 32 982 32 2747
Cuts at: 8952 8984 3166
Size: 32 3174
Fragments arranged by size:
3174 2747 1759 982 170 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8992
XbaI T'CTAG_A
Cuts at: 6328 6328
Size: 8992
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2548 5257 5433 2548
Size: 2709 176 6107
Fragments arranged by size:
6107 2709 176
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8992
XmaI C'CCGG_G
Cuts at: 1870 6317 1870
Size: 4447 4545
XmnI GAAnn'nnTTC
Cuts at: 2072 6158 7121 2072
Size: 4086 963 3943
Fragments arranged by size:
4086 3943 963
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6800 276
Size: 53 83 186 936 5266 2468
Fragments arranged by size:
5266 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PspXI PsrI RsrII SacII SfiI SfoI
SrfI SwaI