Restriction map for
pFN21AA0925
(Circular) (Six-base) MAPSORT of: pFN21AA0925.seq Check: 3117 from: 1 to: 6130
pFN21AA0925 6130 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3940 278
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2049 3473 1937
Size: 112 1424 4594
Fragments arranged by size:
4594 1424 112
Acc65I G'GTAC_C
Cuts at: 3451 3451
Size: 6130
AclI AA'CG_TT
Cuts at: 1563 4257 4630 1563
Size: 2694 373 3063
Fragments arranged by size:
3063 2694 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2375 4193 5577 5845 1632
Size: 743 1818 1384 268 1917
Fragments arranged by size:
1917 1818 1384 743 268
AfeI AGC'GCT
Cuts at: 2455 2455
Size: 6130
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5933
AflIII A'CryG_T
Cuts at: 1203 2316 5029 6006 1203
Size: 1113 2713 977 1327
Fragments arranged by size:
2713 1327 1113 977
AgeI A'CCGG_T
Cuts at: 4961 4961
Size: 6130
AhdI GACnn_n'nnGTC
Cuts at: 4859 4859
Size: 6130
AleI CACnn'nnGTG
Cuts at: 1166 2124 2321 2531 1166
Size: 958 197 210 4765
Fragments arranged by size:
4765 958 210 197
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6130
AlwNI CAG_nnn'CTG
Cuts at: 3873 5445 3873
Size: 1572 4558
ApaLI G'TGCA_C
Cuts at: 2108 4187 5343 2108
Size: 2079 1156 2895
Fragments arranged by size:
2895 2079 1156
ApoI r'AATT_y
Cuts at: 1493 3439 3665 3817 5926 1493
Size: 1946 226 152 2109 1697
Fragments arranged by size:
2109 1946 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4684 160
Size: 872 3652 1606
Fragments arranged by size:
3652 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
AvaI C'yCGr_G
Cuts at: 1870 1943 2342 3455 1870
Size: 73 399 1113 4545
Fragments arranged by size:
4545 1113 399 73
AvrII C'CTAG_G
Cuts at: 2953 2953
Size: 6130
BamHI G'GATC_C
Cuts at: 2088 3460 3827 2088
Size: 1372 367 4391
Fragments arranged by size:
4391 1372 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2424 3451 4906 618
Size: 325 217 627 637 1027 1455 1842
Fragments arranged by size:
1842 1455 1027 637 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2616 2799 3045 3303 3449 1391
Size: 1225 183 246 258 146 4072
Fragments arranged by size:
4072 1225 258 246 183 146
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3093 928
Size: 971 1194 3965
Fragments arranged by size:
3965 1194 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4321 4355 1903
Size: 34 2384 34 3678
Fragments arranged by size:
3678 2384 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4022 5232 4022
Size: 1210 4920
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6130
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2250 4741 136
Size: 107 122 71 631 605 578 2491 1525
Fragments arranged by size:
2491 1525 631 605 578 122 107 71
BglII A'GATC_T
Cuts at: 6125 6125
Size: 6130
BlpI GC'TnA_GC
Cuts at: 3547 3547
Size: 6130
Bme1580I G_kGCm'C
Cuts at: 1790 2112 2397 3063 4191 5347 1790
Size: 322 285 666 1128 1156 2573
Fragments arranged by size:
2573 1156 1128 666 322 285
BmgBI CAC'GTC
Cuts at: 1318 2319 1318
Size: 1001 5129
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3861 4819 442
Size: 416 53 83 399 24 2444 958 1753
Fragments arranged by size:
2444 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6130
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2635 2779 2947 3064 2635
Size: 144 168 117 5701
Fragments arranged by size:
5701 168 144 117
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2219 2402 2701 3371 4790 1239
Size: 980 183 299 670 1419 2579
Fragments arranged by size:
2579 1419 980 670 299 183
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3011 4255 5120 5418 3011
Size: 1244 865 298 3723
Fragments arranged by size:
3723 1244 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2719 4793 882
Size: 617 1220 2074 2219
Fragments arranged by size:
2219 2074 1220 617
BsaAI yAC'GTr
Cuts at: 493 1204 2583 3921 493
Size: 711 1379 1338 2702
Fragments arranged by size:
2702 1379 1338 711
BsaBI GATnn'nnATC
Cuts at: 2093 6124 2093
Size: 4031 2099
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2014 3937 4319 4954
Size: 53 83 186 936 481 1923 382 635
Cuts at: 4954 275
Size: 1451
Fragments arranged by size:
1923 1451 936 635 481 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2040 2874 4563 4961 5235 5382 5793 2040
Size: 834 1689 398 274 147 411 2377
Fragments arranged by size:
2377 1689 834 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2015 2045 2303 2333 1178
Size: 30 165 30 612 30 258 30 4975
Fragments arranged by size:
4975 612 258 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2690 3365 1186
Size: 1504 675 3951
Fragments arranged by size:
3951 1504 675
BseYI C'CCAG_C
Cuts at: 2935 3142 5333 5829 2935
Size: 207 2191 496 3236
Fragments arranged by size:
3236 2191 496 207
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2372 1163
Size: 573 636 4921
Fragments arranged by size:
4921 636 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3580 4341 5369 1540
Size: 275 186 1579 761 1028 2301
Fragments arranged by size:
2301 1579 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2076 2112 2309 3303 4191 4276 5347 2076
Size: 36 197 994 888 85 1071 2859
Fragments arranged by size:
2859 1071 994 888 197 85 36
BsmI GAATG_Cn'
Cuts at: 3375 3641 3734 3375
Size: 266 93 5771
Fragments arranged by size:
5771 266 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2112 2309 2397 2616 2799 3045
Size: 399 286 36 197 88 219 183 246
Cuts at: 3045 3063 3303 3449 4191 4276 5347 1391
Size: 18 240 146 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 286 246 240 219
197 183 146 88 85 36 18
BspEI T'CCGG_A
Cuts at: 2040 5793 2040
Size: 3753 2377
BspHI T'CATG_A
Cuts at: 4019 5749 4019
Size: 1730 4400
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3485 844
Size: 2641 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2619 2931 4017 1394
Size: 429 796 312 1086 3507
Fragments arranged by size:
3507 1086 796 429 312
BsrDI GCAATG_nn'
Cuts at: 65 1248 2260 2295 4625 4799 65
Size: 1183 1012 35 2330 174 1396
Fragments arranged by size:
2330 1396 1183 1012 174 35
BsrFI r'CCGG_y
Cuts at: 1700 1810 2400 2503 4774 4961 1700
Size: 110 590 103 2271 187 2869
Fragments arranged by size:
2869 2271 590 187 110 103
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6130
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6130
BssSI C'ACGA_G
Cuts at: 2302 4190 5202 2302
Size: 1888 1012 3230
Fragments arranged by size:
3230 1888 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6130
BstBI TT'CG_AA
Cuts at: 3307 4934 3307
Size: 1627 4503
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6130
BstYI r'GATC_y
Cuts at: 1972 2088 2328 2337 3160 3460 3827 4212 4229
Size: 116 240 9 823 300 367 385 17
Cuts at: 4229 5681 5767 5779 6125 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 823 385 367 346 300 240
116 86 17 12 9
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5578
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5391
Fragments arranged by size:
5391 683 56
BtsI GCAGTG_nn'
Cuts at: 2074 3646 4440 4460 2074
Size: 1572 794 20 3744
Fragments arranged by size:
3744 1572 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6130
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6095
DraI TTT'AAA
Cuts at: 3434 3434
Size: 6130
DraIII CAC_nnn'GTG
Cuts at: 2583 2662 2583
Size: 79 6051
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2471 5137 809
Size: 507 1155 2666 1802
Fragments arranged by size:
2666 1802 1155 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2061 2148 2242
Size: 54 1425 50 275 18 231 87 94
Cuts at: 2242 2623 3577 4466 6095 8
Size: 381 954 889 1629 43
Fragments arranged by size:
1629 1425 954 889 381 275 231 94
87 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3577 1537
Size: 275 1765 4090
Fragments arranged by size:
4090 1765 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2775 3233 4060 1353
Size: 1422 458 827 3423
Fragments arranged by size:
3423 1422 827 458
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4717 5091 5237 387
Size: 1064 138 3128 374 146 1280
Fragments arranged by size:
3128 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3301 3301
Size: 6130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2219 2375 2402 2701 3371 4193 4790
Size: 393 587 156 27 299 670 822 597
Cuts at: 4790 5577 5845 1239
Size: 787 268 1524
Fragments arranged by size:
1524 822 787 670 597 587 393 299
268 156 27
EcoO109I rG'GnC_Cy
Cuts at: 2473 2758 3031 2473
Size: 285 273 5572
Fragments arranged by size:
5572 285 273
EcoRI G'AATT_C
Cuts at: 3439 3817 3439
Size: 378 5752
EcoRV GAT'ATC
Cuts at: 2266 2266
Size: 6130
FspI TGC'GCA
Cuts at: 3410 4636 3410
Size: 1226 4904
HaeII r_GCGC'y
Cuts at: 1678 2378 2457 5277 1678
Size: 700 79 2820 2531
Fragments arranged by size:
2820 2531 700 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2303 2335 2783 2815 4771
Size: 32 135 32 731 32 448 32 1956
Cuts at: 4771 4803 4845 4877 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1956 731 448 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2134 3253 3474 3720 669
Size: 988 281 196 1119 221 246 3079
Fragments arranged by size:
3079 1119 988 281 246 221 196
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6130
HpaI GTT'AAC
Cuts at: 3720 3720
Size: 6130
KpnI G_GTAC'C
Cuts at: 3455 3455
Size: 6130
MfeI C'AATT_G
Cuts at: 3729 3729
Size: 6130
MluI A'CGCG_T
Cuts at: 6006 6006
Size: 6130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5244 5428 1305
Size: 3939 184 2007
Fragments arranged by size:
3939 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2063 2150 10
Size: 54 1425 343 231 87 3990
Fragments arranged by size:
3990 1425 343 231 87 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2124 2321 2529 2531 2837 3050 3056
Size: 648 958 197 208 2 306 213 6
Cuts at: 3056 4088 4447 4606 518
Size: 1032 359 159 2042
Fragments arranged by size:
2042 1032 958 648 359 306 213 208
197 159 6 2
MspA1I CmG'CkG
Cuts at: 1606 3391 3546 4223 5371 5616 1606
Size: 1785 155 677 1148 245 2120
Fragments arranged by size:
2120 1785 1148 677 245 155
NaeI GCC'GGC
Cuts at: 2402 2402
Size: 6130
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5578
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6130
NgoMIV G'CCGG_C
Cuts at: 2400 2400
Size: 6130
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6130
NotI GC'GGCC_GC
Cuts at: 3577 3577
Size: 6130
NspI r_CATG'y
Cuts at: 1132 1229 2548 2842 3488 5033 1132
Size: 97 1319 294 646 1545 2229
Fragments arranged by size:
2229 1545 1319 646 294 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6130
PciI A'CATG_T
Cuts at: 5029 5029
Size: 6130
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 5228
PfoI T'CCnGG_A
Cuts at: 2379 2379
Size: 6130
PmeI GTTT'AAAC
Cuts at: 3434 3434
Size: 6130
PmlI CAC'GTG
Cuts at: 2583 2583
Size: 6130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4199 4231 5712 5744 4199
Size: 32 1481 32 4585
Fragments arranged by size:
4585 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2473 2758 2473
Size: 285 5845
PshAI GACnn'nnGTC
Cuts at: 1613 2711 1613
Size: 1098 5032
PsiI TTA'TAA
Cuts at: 3700 3700
Size: 6130
PstI C_TGCA'G
Cuts at: 830 2637 3482 830
Size: 1807 845 3478
Fragments arranged by size:
3478 1807 845
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6130
PvuII CAG'CTG
Cuts at: 3391 3391
Size: 6130
SacI G_AGCT'C
Cuts at: 3303 3303
Size: 6130
SalI G'TCGA_C
Cuts at: 1936 3472 1936
Size: 1536 4594
SbfI CC_TGCA'GG
Cuts at: 3482 3482
Size: 6130
ScaI AGT'ACT
Cuts at: 4378 6090 4378
Size: 1712 4418
SfcI C'TryA_G
Cuts at: 826 1045 2633 3478 4613 5294 5485 826
Size: 219 1588 845 1135 681 191 1471
Fragments arranged by size:
1588 1471 1135 845 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6130
SmaI CCC'GGG
Cuts at: 1872 3457 1872
Size: 1585 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3026 4234 5135 5397 820
Size: 197 926 1083 1208 901 262 1553
Fragments arranged by size:
1553 1208 1083 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3921 493
Size: 3428 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6130
SphI G_CATG'C
Cuts at: 1132 3488 1132
Size: 2356 3774
SspI AAT'ATT
Cuts at: 5 52 4054 5
Size: 47 4002 2081
Fragments arranged by size:
4002 2081 47
StuI AGG'CCT
Cuts at: 1517 2952 1517
Size: 1435 4695
StyI C'CwwG_G
Cuts at: 513 1065 2232 2953 3569 513
Size: 552 1167 721 616 3074
Fragments arranged by size:
3074 1167 721 616 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2486 2849 3298 4157 4174 4327 4512
Size: 1255 906 363 449 859 17 153 185
Cuts at: 4512 4957 325
Size: 445 1498
Fragments arranged by size:
1498 1255 906 859 449 445 363 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4376 6088 96
Size: 274 80 33 51 1443 2399 1712 138
Fragments arranged by size:
2399 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6090 6122 6090
Size: 32 6098
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6130
XbaI T'CTAG_A
Cuts at: 3466 3466
Size: 6130
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2974 2974
Size: 6130
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6130
XmaI C'CCGG_G
Cuts at: 1870 3455 1870
Size: 1585 4545
XmnI GAAnn'nnTTC
Cuts at: 2279 4259 2279
Size: 1980 4150
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3938 276
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI FalI
FseI FspAI KasI NarI NruI NsiI PacI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI