Restriction map for
pFN21AA0917
(Circular) (Six-base) MAPSORT of: pFN21AA0917.seq Check: 9067 from: 1 to: 6631
pFN21AA0917 6631 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4441 278
Size: 53 83 186 936 2905 2468
Fragments arranged by size:
2905 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2271 3974 1937
Size: 334 1703 4594
Fragments arranged by size:
4594 1703 334
Acc65I G'GTAC_C
Cuts at: 3952 3952
Size: 6631
AclI AA'CG_TT
Cuts at: 1563 4758 5131 1563
Size: 3195 373 3063
Fragments arranged by size:
3195 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2310 2880 4694 6078 6346 1632
Size: 678 570 1814 1384 268 1917
Fragments arranged by size:
1917 1814 1384 678 570 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6434
AflIII A'CryG_T
Cuts at: 1203 5530 6507 1203
Size: 4327 977 1327
Fragments arranged by size:
4327 1327 977
AgeI A'CCGG_T
Cuts at: 5462 5462
Size: 6631
AhdI GACnn_n'nnGTC
Cuts at: 5360 5360
Size: 6631
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6631
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6631
AlwNI CAG_nnn'CTG
Cuts at: 3338 4374 5946 3338
Size: 1036 1572 4023
Fragments arranged by size:
4023 1572 1036
ApaLI G'TGCA_C
Cuts at: 2846 4688 5844 2846
Size: 1842 1156 3633
Fragments arranged by size:
3633 1842 1156
ApoI r'AATT_y
Cuts at: 1493 2086 2353 2975 3940 4166 4318 6427 1493
Size: 593 267 622 965 226 152 2109 1697
Fragments arranged by size:
2109 1697 965 622 593 267 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5185 160
Size: 872 4153 1606
Fragments arranged by size:
4153 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6631
AvaI C'yCGr_G
Cuts at: 1870 1943 3956 1870
Size: 73 2013 4545
Fragments arranged by size:
4545 2013 73
BamHI G'GATC_C
Cuts at: 3961 4328 3961
Size: 367 6264
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3952 5407 618
Size: 325 217 627 2165 1455 1842
Fragments arranged by size:
2165 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3950 1391
Size: 2559 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5660
BbvCI CC'TCA_GC
Cuts at: 3514 3514
Size: 6631
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4822 4856 1903
Size: 34 2885 34 3678
Fragments arranged by size:
3678 2885 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4523 5733 4523
Size: 1210 5421
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6631
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5242 136
Size: 107 122 71 631 605 337 3233 1525
Fragments arranged by size:
3233 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6626 6626
Size: 6631
BlpI GC'TnA_GC
Cuts at: 3144 4048 3144
Size: 904 5727
Bme1580I G_kGCm'C
Cuts at: 1790 2850 4692 5848 1790
Size: 1060 1842 1156 2573
Fragments arranged by size:
2573 1842 1156 1060
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6631
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4362 5320 442
Size: 416 53 83 399 24 2945 958 1753
Fragments arranged by size:
2945 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6631
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2898 3336 5291 1239
Size: 1659 438 1955 2579
Fragments arranged by size:
2579 1955 1659 438
Bpu10I CC'TnA_GC
Cuts at: 3514 3514
Size: 6631
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3203 4756 5621 5919 3203
Size: 1553 865 298 3915
Fragments arranged by size:
3915 1553 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5294 882
Size: 617 3795 2219
Fragments arranged by size:
3795 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4422 493
Size: 711 3218 2702
Fragments arranged by size:
3218 2702 711
BsaBI GATnn'nnATC
Cuts at: 2244 6625 2244
Size: 4381 2250
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4438 4820 5455 275
Size: 53 83 186 936 2905 382 635 1451
Fragments arranged by size:
2905 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2965 5064 5462 5736 5883 6294 2965
Size: 2099 398 274 147 411 3302
Fragments arranged by size:
3302 2099 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2112 2142 3662 3692 1178
Size: 30 165 30 709 30 1520 30 4117
Fragments arranged by size:
4117 1520 709 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2166 3810 1186
Size: 980 1644 4007
Fragments arranged by size:
4007 1644 980
BseYI C'CCAG_C
Cuts at: 5834 6330 5834
Size: 496 6135
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2439 1163
Size: 573 703 5355
Fragments arranged by size:
5355 703 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4081 4842 5870 1540
Size: 275 186 2080 761 1028 2301
Fragments arranged by size:
2301 2080 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2850 3055 4692 4777 5848 2850
Size: 205 1637 85 1071 3633
Fragments arranged by size:
3633 1637 1071 205 85
BsmI GAATG_Cn'
Cuts at: 4142 4235 4142
Size: 93 6538
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2850 3055 3950 4692 4777 5848 1391
Size: 399 1060 205 895 742 85 1071 2174
Fragments arranged by size:
2174 1071 1060 895 742 399 205 85
BspEI T'CCGG_A
Cuts at: 6294 6294
Size: 6631
BspHI T'CATG_A
Cuts at: 3573 4520 6250 3573
Size: 947 1730 3954
Fragments arranged by size:
3954 1730 947
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3986 844
Size: 3142 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4518 1394
Size: 429 2695 3507
Fragments arranged by size:
3507 2695 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3739 5126 5300 65
Size: 1183 2491 1387 174 1396
Fragments arranged by size:
2491 1396 1387 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5275 5462 1700
Size: 110 3465 187 2869
Fragments arranged by size:
3465 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6631
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6631
BssSI C'ACGA_G
Cuts at: 3567 4691 5703 3567
Size: 1124 1012 4495
Fragments arranged by size:
4495 1124 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2417 794
Size: 1623 5008
BstBI TT'CG_AA
Cuts at: 2323 5435 2323
Size: 3112 3519
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6631
BstYI r'GATC_y
Cuts at: 1972 2245 2317 3961 4328 4713 4730 6182 6268
Size: 273 72 1644 367 385 17 1452 86
Cuts at: 6268 6280 6626 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1644 1452 385 367 346 273 86
72 17 12
BstZ17I GTA'TAC
Cuts at: 2272 2272
Size: 6631
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5140
Fragments arranged by size:
5140 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5892
Fragments arranged by size:
5892 683 56
BtsI GCAGTG_nn'
Cuts at: 2676 3222 3880 4147 4941 4961 2676
Size: 546 658 267 794 20 4346
Fragments arranged by size:
4346 794 658 546 267 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6631
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6596
DraI TTT'AAA
Cuts at: 2352 2871 3935 2352
Size: 519 1064 5048
Fragments arranged by size:
5048 1064 519
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5638 809
Size: 507 4322 1802
Fragments arranged by size:
4322 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2158 2749 3522
Size: 54 1425 50 275 18 328 591 773
Cuts at: 3522 4078 4967 6596 8
Size: 556 889 1629 43
Fragments arranged by size:
1629 1425 889 773 591 556 328 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4078 1537
Size: 275 2266 4090
Fragments arranged by size:
4090 2266 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2600 3321 4561 1353
Size: 1247 721 1240 3423
Fragments arranged by size:
3423 1247 1240 721
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5218 5592 5738 387
Size: 1064 138 3629 374 146 1280
Fragments arranged by size:
3629 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2310 2880 2898 3336 4694 5291 6078
Size: 393 678 570 18 438 1358 597 787
Cuts at: 6078 6346 1239
Size: 268 1524
Fragments arranged by size:
1524 1358 787 678 597 570 438 393
268 18
EcoNI CCTnn'n_nnAGG
Cuts at: 3342 3342
Size: 6631
EcoO109I rG'GnC_Cy
Cuts at: 2769 2769
Size: 6631
EcoRI G'AATT_C
Cuts at: 3940 4318 3940
Size: 378 6253
EcoRV GAT'ATC
Cuts at: 2545 2545
Size: 6631
FspI TGC'GCA
Cuts at: 5137 5137
Size: 6631
HaeII r_GCGC'y
Cuts at: 1678 5778 1678
Size: 4100 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2156 2188 3314 3346 3662
Size: 32 135 32 584 32 1126 32 316
Cuts at: 3662 3694 5272 5304 5346 5378 1373
Size: 32 1578 32 42 32 2626
Fragments arranged by size:
2626 1578 1126 584 316 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2587 2788 3829 3975 4221 669
Size: 988 281 649 201 1041 146 246 3079
Fragments arranged by size:
3079 1041 988 649 281 246 201 146
HindIII A'AGCT_T
Cuts at: 748 3438 748
Size: 2690 3941
HpaI GTT'AAC
Cuts at: 4221 4221
Size: 6631
KpnI G_GTAC'C
Cuts at: 3956 3956
Size: 6631
MfeI C'AATT_G
Cuts at: 4230 4230
Size: 6631
MluI A'CGCG_T
Cuts at: 6507 6507
Size: 6631
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5745 5929 1305
Size: 4440 184 2007
Fragments arranged by size:
4440 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2160 2751 10
Size: 54 1425 343 328 591 3890
Fragments arranged by size:
3890 1425 591 343 328 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2558 3122 3213 4589 4948 5107 518
Size: 648 1392 564 91 1376 359 159 2042
Fragments arranged by size:
2042 1392 1376 648 564 359 159 91
MspA1I CmG'CkG
Cuts at: 1606 2918 3158 4047 4724 5872 6117 1606
Size: 1312 240 889 677 1148 245 2120
Fragments arranged by size:
2120 1312 1148 889 677 245 240
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5140
Fragments arranged by size:
5140 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6631
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6631
NotI GC'GGCC_GC
Cuts at: 4078 4078
Size: 6631
NspI r_CATG'y
Cuts at: 1132 1229 3989 5534 1132
Size: 97 2760 1545 2229
Fragments arranged by size:
2760 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6631
PciI A'CATG_T
Cuts at: 5530 5530
Size: 6631
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6631
PmeI GTTT'AAAC
Cuts at: 3935 3935
Size: 6631
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4700 4732 6213 6245 4700
Size: 32 1481 32 5086
Fragments arranged by size:
5086 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6631
PsiI TTA'TAA
Cuts at: 4201 4201
Size: 6631
PstI C_TGCA'G
Cuts at: 830 2269 3875 3983 830
Size: 1439 1606 108 3478
Fragments arranged by size:
3478 1606 1439 108
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6631
PvuII CAG'CTG
Cuts at: 2918 3158 2918
Size: 240 6391
SalI G'TCGA_C
Cuts at: 1936 3973 1936
Size: 2037 4594
SbfI CC_TGCA'GG
Cuts at: 3983 3983
Size: 6631
ScaI AGT'ACT
Cuts at: 4879 6591 4879
Size: 1712 4919
SexAI A'CCwGG_T
Cuts at: 3615 3615
Size: 6631
SfcI C'TryA_G
Cuts at: 826 1045 2265 2580 3700 3871 3979 5114 5795
Size: 219 1220 315 1120 171 108 1135 681
Cuts at: 5795 5986 826
Size: 191 1471
Fragments arranged by size:
1471 1220 1135 1120 681 315 219 191
171 108
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6631
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6631
SmaI CCC'GGG
Cuts at: 1872 3958 1872
Size: 2086 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3182 4735 5636 5898 820
Size: 197 926 1239 1553 901 262 1553
Fragments arranged by size:
1553 1553 1239 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4422 493
Size: 3929 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6631
SphI G_CATG'C
Cuts at: 1132 3989 1132
Size: 2857 3774
SspI AAT'ATT
Cuts at: 5 52 2299 4555 5
Size: 47 2247 2256 2081
Fragments arranged by size:
2256 2247 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6631
StyI C'CwwG_G
Cuts at: 513 1065 2004 2436 4070 513
Size: 552 939 432 1634 3074
Fragments arranged by size:
3074 1634 939 552 432
SwaI ATTT'AAAT
Cuts at: 2352 2352
Size: 6631
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2606 4658 4675 4828 5013 5458 325
Size: 1255 1026 2052 17 153 185 445 1498
Fragments arranged by size:
2052 1498 1255 1026 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2854 4877 6589
Size: 274 80 33 51 1443 877 2023 1712
Cuts at: 6589 96
Size: 138
Fragments arranged by size:
2023 1712 1443 877 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6591 6623 6591
Size: 32 6599
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6631
XbaI T'CTAG_A
Cuts at: 3319 3967 3319
Size: 648 5983
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3550 3784 3550
Size: 234 6397
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6631
XmaI C'CCGG_G
Cuts at: 1870 3956 1870
Size: 2086 4545
XmnI GAAnn'nnTTC
Cuts at: 3024 4760 3024
Size: 1736 4895
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4439 276
Size: 53 83 186 936 2905 2468
Fragments arranged by size:
2905 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI