Restriction map for
pFN21AA0913
(Circular) (Six-base) MAPSORT of: pFN21AA0913.seq Check: 6568 from: 1 to: 10093
pFN21AA0913 10093 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2242 3791 6179 6239 6456 6879 7143 2242
Size: 1549 2388 60 217 423 264 5192
Fragments arranged by size:
5192 2388 1549 423 264 217 60
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7903 278
Size: 53 83 186 936 6367 2468
Fragments arranged by size:
6367 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 7436 1937
Size: 5499 4594
Acc65I G'GTAC_C
Cuts at: 2198 7414 2198
Size: 5216 4877
AclI AA'CG_TT
Cuts at: 1563 8220 8593 1563
Size: 6657 373 3063
Fragments arranged by size:
6657 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2926 3383 3729 4208 6156 8156 9540 9808
Size: 1294 457 346 479 1948 2000 1384 268
Cuts at: 9808 1632
Size: 1917
Fragments arranged by size:
2000 1948 1917 1384 1294 479 457 346
268
AfeI AGC'GCT
Cuts at: 2042 7169 2042
Size: 5127 4966
AflII C'TTAA_G
Cuts at: 820 1017 4916 5380 820
Size: 197 3899 464 5533
Fragments arranged by size:
5533 3899 464 197
AflIII A'CryG_T
Cuts at: 1203 4850 8992 9969 1203
Size: 3647 4142 977 1327
Fragments arranged by size:
4142 3647 1327 977
AgeI A'CCGG_T
Cuts at: 8924 8924
Size: 10093
AhdI GACnn_n'nnGTC
Cuts at: 6837 8822 6837
Size: 1985 8108
AleI CACnn'nnGTG
Cuts at: 1166 2472 3192 6347 6626 6707 7137 1166
Size: 1306 720 3155 279 81 430 4122
Fragments arranged by size:
4122 3155 1306 720 430 279 81
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2724 1184
Size: 1540 8553
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4352 4384 4352
Size: 32 10061
AlwNI CAG_nnn'CTG
Cuts at: 2306 2990 3959 4064 4303 4790 4975 5084 5093
Size: 684 969 105 239 487 185 109 9
Cuts at: 5093 5120 6299 7108 7836 9408 2306
Size: 27 1179 809 728 1572 2991
Fragments arranged by size:
2991 1572 1179 969 809 728 684 487
239 185 109 105 27 9
ApaI G_GGCC'C
Cuts at: 2157 2841 3532 3538 5045 5062 5413 6046 6293
Size: 684 691 6 1507 17 351 633 247
Cuts at: 6293 2157
Size: 5957
Fragments arranged by size:
5957 1507 691 684 633 351 247 17
6
ApaLI G'TGCA_C
Cuts at: 7025 8150 9306 7025
Size: 1125 1156 7812
Fragments arranged by size:
7812 1156 1125
ApoI r'AATT_y
Cuts at: 1493 7402 7628 7780 9889 1493
Size: 5909 226 152 2109 1697
Fragments arranged by size:
5909 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8647 160
Size: 872 7615 1606
Fragments arranged by size:
7615 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10093
AvaI C'yCGr_G
Cuts at: 1870 1943 3531 3585 5526 5570 6382 6821 7370
Size: 73 1588 54 1941 44 812 439 549
Cuts at: 7370 7418 1870
Size: 48 4545
Fragments arranged by size:
4545 1941 1588 812 549 439 73 54
48 44
AvrII C'CTAG_G
Cuts at: 6574 6574
Size: 10093
BamHI G'GATC_C
Cuts at: 7423 7790 7423
Size: 367 9726
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2198 2485 3324 3554 4614
Size: 325 217 627 411 287 839 230 1060
Cuts at: 4614 5901 6349 6652 6758 7414 8869 618
Size: 1287 448 303 106 656 1455 1842
Fragments arranged by size:
1842 1455 1287 1060 839 656 627 448
411 325 303 287 230 217 106
BanII G_rGCy'C
Cuts at: 1391 2157 2659 2733 2821 2841 3532 3538 3797
Size: 766 502 74 88 20 691 6 259
Cuts at: 3797 3935 5045 5062 5413 5480 5527 6046 6293
Size: 138 1110 17 351 67 47 519 247
Cuts at: 6293 6583 7067 7412 1391
Size: 290 484 345 4072
Fragments arranged by size:
4072 1110 766 691 519 502 484 351
345 290 259 247 138 88 74 67
47 20 17 6
BbeI G_GCGC'C
Cuts at: 3328 3328
Size: 10093
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2775 2932 2961 3407 3840 4811 5186
Size: 971 876 157 29 446 433 971 375
Cuts at: 5186 5540 5648 6517 928
Size: 354 108 869 4504
Fragments arranged by size:
4504 971 971 876 869 446 433 375
354 157 108 29
BbvCI CC'TCA_GC
Cuts at: 2736 2842 3178 4236 2736
Size: 106 336 1058 8593
Fragments arranged by size:
8593 1058 336 106
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2584 2618 4160 4194 5561 5595 5629
Size: 34 647 34 1542 34 1367 34 34
Cuts at: 5629 5663 8284 8318 1903
Size: 34 2621 34 3678
Fragments arranged by size:
3678 2621 1542 1367 647 34 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4378 7985 9195 4378
Size: 3607 1210 5276
Fragments arranged by size:
5276 3607 1210
BclI T'GATC_A
Cuts at: 1548 4887 5652 1548
Size: 3339 765 5989
Fragments arranged by size:
5989 3339 765
BfrBI ATG'CAT
Cuts at: 6017 6017
Size: 10093
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3547 3972 5057
Size: 107 122 71 631 605 1875 425 1085
Cuts at: 5057 6095 8704 136
Size: 1038 2609 1525
Fragments arranged by size:
2609 1875 1525 1085 1038 631 605 425
122 107 71
BglII A'GATC_T
Cuts at: 4923 10088 4923
Size: 5165 4928
BlpI GC'TnA_GC
Cuts at: 2757 3056 3796 3824 7061 7510 2757
Size: 299 740 28 3237 449 5340
Fragments arranged by size:
5340 3237 740 449 299 28
Bme1580I G_kGCm'C
Cuts at: 1790 2157 2841 3207 3532 3538 5045 5062 5350
Size: 367 684 366 325 6 1507 17 288
Cuts at: 5350 5413 5904 6046 6293 6354 6537 6928 7029
Size: 63 491 142 247 61 183 391 101
Cuts at: 7029 7144 7264 8154 9310 1790
Size: 115 120 890 1156 2573
Fragments arranged by size:
2573 1507 1156 890 684 491 391 367
366 325 288 247 183 142 120 115
101 63 61 17 6
BmgBI CAC'GTC
Cuts at: 1318 2602 4853 1318
Size: 1284 2251 6558
Fragments arranged by size:
6558 2251 1284
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2654 2782 3469
Size: 416 53 83 399 24 1237 128 687
Cuts at: 3469 3776 6404 7824 8782 442
Size: 307 2628 1420 958 1753
Fragments arranged by size:
2628 1753 1420 1237 958 687 416 399
307 128 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4310 1056
Size: 3254 6839
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2062 7134 2062
Size: 5072 5021
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3428 3596 3866 4491 4695 5473 6926 8753
Size: 2189 168 270 625 204 778 1453 1827
Cuts at: 8753 1239
Size: 2579
Fragments arranged by size:
2579 2189 1827 1453 778 625 270 204
168
Bpu10I CC'TnA_GC
Cuts at: 2307 2736 2842 3178 4236 4406 5993 6114 2307
Size: 429 106 336 1058 170 1587 121 6286
Fragments arranged by size:
6286 1587 1058 429 336 170 121 106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4310 4556 6736 7310 8218 9083 9381 4310
Size: 246 2180 574 908 865 298 5022
Fragments arranged by size:
5022 2180 908 865 574 298 246
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5028 5383 7369 8756 882
Size: 617 3529 355 1986 1387 2219
Fragments arranged by size:
3529 2219 1986 1387 617 355
BsaAI yAC'GTr
Cuts at: 493 1204 3173 5810 7024 7884 493
Size: 711 1969 2637 1214 860 2702
Fragments arranged by size:
2702 2637 1969 1214 860 711
BsaBI GATnn'nnATC
Cuts at: 10087 10087
Size: 10093
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3042 3325 7283 7900
Size: 53 83 186 936 1509 283 3958 617
Cuts at: 7900 8282 8917 275
Size: 382 635 1451
Fragments arranged by size:
3958 1509 1451 936 635 617 382 283
186 83 53
BsaWI w'CCGG_w
Cuts at: 2821 7254 8526 8924 9198 9345 9756 2821
Size: 4433 1272 398 274 147 411 3158
Fragments arranged by size:
4433 3158 1272 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2000 2030 4208 4238 6935
Size: 30 165 30 597 30 2178 30 2697
Cuts at: 6935 6965 1178
Size: 30 4306
Fragments arranged by size:
4306 2697 2178 597 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2087 4091 4178 4778 5470 5535 6594 6839
Size: 901 2004 87 600 692 65 1059 245
Cuts at: 6839 6842 1186
Size: 3 4437
Fragments arranged by size:
4437 2004 1059 901 692 600 245 87
65 3
BseYI C'CCAG_C
Cuts at: 2579 3318 3525 4124 4157 4181 5120 5406 5892
Size: 739 207 599 33 24 939 286 486
Cuts at: 5892 6260 6478 6536 6788 6913 6991 7009 9296
Size: 368 218 58 252 125 78 18 2287
Cuts at: 9296 9792 2579
Size: 496 2880
Fragments arranged by size:
2880 2287 939 739 599 496 486 368
286 252 218 207 125 78 58 33
24 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2578 2894 3291 5601 5720 5762 6071
Size: 573 842 316 397 2310 119 42 309
Cuts at: 6071 6404 6848 7184 1163
Size: 333 444 336 4072
Fragments arranged by size:
4072 2310 842 573 444 397 336 333
316 309 119 42
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7543 8304 9332 1540
Size: 275 186 5542 761 1028 2301
Fragments arranged by size:
5542 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2733 2821 4170 4455 4711 7029 8154 8239 9310
Size: 88 1349 285 256 2318 1125 85 1071
Cuts at: 9310 2733
Size: 3516
Fragments arranged by size:
3516 2318 1349 1125 1071 285 256 88
85
BsiWI C'GTAC_G
Cuts at: 3169 3169
Size: 10093
BsmI GAATG_Cn'
Cuts at: 2211 4114 5894 6569 6900 7252 7604 7697 2211
Size: 1903 1780 675 331 352 352 93 4607
Fragments arranged by size:
4607 1903 1780 675 352 352 331 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2768 4283 7088 2768
Size: 1515 2805 5773
Fragments arranged by size:
5773 2805 1515
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2157 2659 2733 2821 2841 3207 3532
Size: 399 367 502 74 88 20 366 325
Cuts at: 3532 3538 3797 3935 4170 4455 4711 5045 5062
Size: 6 259 138 235 285 256 334 17
Cuts at: 5062 5350 5413 5480 5527 5904 6046 6293 6354
Size: 288 63 67 47 377 142 247 61
Cuts at: 6354 6537 6583 6928 7029 7067 7144 7264 7412
Size: 183 46 345 101 38 77 120 148
Cuts at: 7412 8154 8239 9310 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 502 399 377 367 366
345 334 325 288 285 259 256 247
235 183 148 142 138 120 101 88
85 77 74 67 63 61 47 46
38 20 17 6
BspEI T'CCGG_A
Cuts at: 2821 7254 9756 2821
Size: 4433 2502 3158
Fragments arranged by size:
4433 3158 2502
BspHI T'CATG_A
Cuts at: 7982 9712 7982
Size: 1730 8363
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2242 3060 3791 5541 5742 6179 6239 6456
Size: 1398 818 731 1750 201 437 60 217
Cuts at: 6456 6879 7143 7448 844
Size: 423 264 305 3489
Fragments arranged by size:
3489 1750 1398 818 731 437 423 305
264 217 201 60
BsrBI CCG'CTC
Cuts at: 1394 1823 3412 3564 6804 6957 7118 7980 1394
Size: 429 1589 152 3240 153 161 862 3507
Fragments arranged by size:
3507 3240 1589 862 429 161 153 152
BsrDI GCAATG_nn'
Cuts at: 65 1248 2450 3100 4039 8588 8762 65
Size: 1183 1202 650 939 4549 174 1396
Fragments arranged by size:
4549 1396 1202 1183 939 650 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3591 6304 8737 8924 1700
Size: 110 1781 2713 2433 187 2869
Fragments arranged by size:
2869 2713 2433 1781 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 10093
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10093
BssSI C'ACGA_G
Cuts at: 4372 8153 9165 4372
Size: 3781 1012 5300
Fragments arranged by size:
5300 3781 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3245 4064 4787 4790 5093 6871 794
Size: 2451 819 723 3 303 1778 4016
Fragments arranged by size:
4016 2451 1778 819 723 303 3
BstBI TT'CG_AA
Cuts at: 8897 8897
Size: 10093
BstEII G'GTnAC_C
Cuts at: 1184 4865 1184
Size: 3681 6412
BstXI CCAn_nnnn'nTGG
Cuts at: 3766 4525 6413 6709 6796 3766
Size: 759 1888 296 87 7063
Fragments arranged by size:
7063 1888 759 296 87
BstYI r'GATC_y
Cuts at: 1972 2742 4300 4578 4923 6033 7423 7790 8175
Size: 770 1558 278 345 1110 1390 367 385
Cuts at: 8175 8192 9644 9730 9742 10088 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1558 1452 1390 1110 770 385 367
346 345 278 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5077 5704 5916 6276 5077
Size: 627 212 360 8894
Fragments arranged by size:
8894 627 360 212
BtgI C'CryG_G
Cuts at: 513 1065 2004 2973 5850 6648 7287 513
Size: 552 939 969 2877 798 639 3319
Fragments arranged by size:
3319 2877 969 939 798 639 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9354
Fragments arranged by size:
9354 683 56
BtsI GCAGTG_nn'
Cuts at: 2197 2398 2906 3199 3359 3787 4270 5350 6059
Size: 201 508 293 160 428 483 1080 709
Cuts at: 6059 6105 6333 6429 6621 6695 6727 6764 7609
Size: 46 228 96 192 74 32 37 845
Cuts at: 7609 8403 8423 2197
Size: 794 20 3867
Fragments arranged by size:
3867 1080 845 794 709 508 483 428
293 228 201 192 160 96 74 46
37 32 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10093
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2235 2270 2902 2937 529
Size: 35 1671 35 632 35 7685
Fragments arranged by size:
7685 1671 632 35 35 35
DraI TTT'AAA
Cuts at: 7397 7397
Size: 10093
DraIII CAC_nnn'GTG
Cuts at: 2605 3282 3770 4649 6557 2605
Size: 677 488 879 1908 6141
Fragments arranged by size:
6141 1908 879 677 488
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3272 9100 809
Size: 507 1956 5828 1802
Fragments arranged by size:
5828 1956 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2382 3291 3431
Size: 54 1425 50 275 18 552 909 140
Cuts at: 3431 3548 3858 4680 5328 6403 7377 7540 8429
Size: 117 310 822 648 1075 974 163 889
Cuts at: 8429 10058 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1075 974 909 889 822 648
552 310 275 163 140 117 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7540 1537
Size: 275 5728 4090
Fragments arranged by size:
5728 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2349 2429 2624 3422 3459 4131 5464 6099
Size: 996 80 195 798 37 672 1333 635
Cuts at: 6099 8023 1353
Size: 1924 3423
Fragments arranged by size:
3423 1924 1333 996 798 672 635 195
80 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2196 3822 8680 9054 9200 387
Size: 1064 138 607 1626 4858 374 146 1280
Fragments arranged by size:
4858 1626 1280 1064 607 374 146 138
EcoICRI GAG'CTC
Cuts at: 2731 2819 2731
Size: 88 10005
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2926 3383 3428 3596 3729 3866 4208
Size: 393 1294 457 45 168 133 137 342
Cuts at: 4208 4491 4695 5473 6156 6926 8156 8753 9540
Size: 283 204 778 683 770 1230 597 787
Cuts at: 9540 9808 1239
Size: 268 1524
Fragments arranged by size:
1524 1294 1230 787 778 770 683 597
457 393 342 283 268 204 168 137
133 45
EcoNI CCTnn'n_nnAGG
Cuts at: 2508 4409 2508
Size: 1901 8192
EcoO109I rG'GnC_Cy
Cuts at: 2153 2446 2514 2837 2838 3535 3571 3670 3689
Size: 293 68 323 1 697 36 99 19
Cuts at: 3689 4240 5041 5058 5059 5090 5129 5292 5410
Size: 551 801 17 1 31 39 163 118
Cuts at: 5410 5720 6043 6289 2153
Size: 310 323 246 5957
Fragments arranged by size:
5957 801 697 551 323 323 310 293
246 163 118 99 68 39 36 31
19 17 1 1
EcoRI G'AATT_C
Cuts at: 7402 7780 7402
Size: 378 9715
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4435 4942 4435
Size: 507 9586
FspI TGC'GCA
Cuts at: 4251 8599 4251
Size: 4348 5745
FspAI rTGC'GCAy
Cuts at: 4251 4251
Size: 10093
HaeII r_GCGC'y
Cuts at: 1678 2044 3328 7171 7272 9240 1678
Size: 366 1284 3843 101 1968 2531
Fragments arranged by size:
3843 2531 1968 1284 366 101
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2208 2240 2488 2520 2646
Size: 32 135 32 636 32 248 32 126
Cuts at: 2646 2660 2678 2692 2939 2971 5909 5927 5941
Size: 14 18 14 247 32 2938 18 14
Cuts at: 5941 5959 6823 6855 6901 6933 8734 8766 8808
Size: 18 864 32 46 32 1801 32 42
Cuts at: 8808 8840 1373
Size: 32 2626
Fragments arranged by size:
2938 2626 1801 864 636 248 247 135
126 46 42 32 32 32 32 32
32 32 32 32 18 18 18 14
14 14
HincII GTy'rAC
Cuts at: 669 1657 1938 2793 4428 7437 7683 669
Size: 988 281 855 1635 3009 246 3079
Fragments arranged by size:
3079 3009 1635 988 855 281 246
HindIII A'AGCT_T
Cuts at: 748 4913 748
Size: 4165 5928
HpaI GTT'AAC
Cuts at: 7683 7683
Size: 10093
KasI G'GCGC_C
Cuts at: 3324 3324
Size: 10093
KpnI G_GTAC'C
Cuts at: 2202 7418 2202
Size: 5216 4877
MfeI C'AATT_G
Cuts at: 7692 7692
Size: 10093
MluI A'CGCG_T
Cuts at: 9969 9969
Size: 10093
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3090 4051 6060 6871 9207 9391 1305
Size: 1785 961 2009 811 2336 184 2007
Fragments arranged by size:
2336 2009 2007 1785 961 811 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2384 4682 5330 6405 7379
Size: 54 1425 343 552 2298 648 1075 974
Cuts at: 7379 10
Size: 2724
Fragments arranged by size:
2724 2298 1425 1075 974 648 552 343
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2472 3192 3249 4251 5324 6012 6098
Size: 648 1306 720 57 1002 1073 688 86
Cuts at: 6098 6347 6362 6626 6707 6873 7137 7292 8051
Size: 249 15 264 81 166 264 155 759
Cuts at: 8051 8410 8569 518
Size: 359 159 2042
Fragments arranged by size:
2042 1306 1073 1002 759 720 688 648
359 264 264 249 166 159 155 86
81 57 15
MspA1I CmG'CkG
Cuts at: 1606 2150 2544 2565 2698 2990 3322 3346 3437
Size: 544 394 21 133 292 332 24 91
Cuts at: 3437 3498 3620 3685 3707 5615 6040 6320 7105
Size: 61 122 65 22 1908 425 280 785
Cuts at: 7105 7360 7509 8186 9334 9579 1606
Size: 255 149 677 1148 245 2120
Fragments arranged by size:
2120 1908 1148 785 677 544 425 394
332 292 280 255 245 149 133 122
91 65 61 24 22 21
NarI GG'CG_CC
Cuts at: 3325 3325
Size: 10093
NcoI C'CATG_G
Cuts at: 513 1065 2004 5850 6648 7287 513
Size: 552 939 3846 798 639 3319
Fragments arranged by size:
3846 3319 939 798 639 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10093
NheI G'CTAG_C
Cuts at: 1052 4306 1052
Size: 3254 6839
NotI GC'GGCC_GC
Cuts at: 7540 7540
Size: 10093
NsiI A_TGCA'T
Cuts at: 6019 6019
Size: 10093
NspI r_CATG'y
Cuts at: 1132 1229 2434 3006 3262 4250 4483 6511 6878
Size: 97 1205 572 256 988 233 2028 367
Cuts at: 6878 7189 7451 8996 1132
Size: 311 262 1545 2229
Fragments arranged by size:
2229 2028 1545 1205 988 572 367 311
262 256 233 97
PasI CC'CwG_GG
Cuts at: 1348 3892 5054 5473 6293 1348
Size: 2544 1162 419 820 5148
Fragments arranged by size:
5148 2544 1162 820 419
PciI A'CATG_T
Cuts at: 8992 8992
Size: 10093
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2605 3482 4462 5421 5851 7159 1108
Size: 1497 877 980 959 430 1308 4042
Fragments arranged by size:
4042 1497 1308 980 959 877 430
PfoI T'CCnGG_A
Cuts at: 2766 5831 6388 2766
Size: 3065 557 6471
Fragments arranged by size:
6471 3065 557
PmeI GTTT'AAAC
Cuts at: 7397 7397
Size: 10093
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4818 4850 8162 8194 9675 9707 4818
Size: 32 3312 32 1481 32 5204
Fragments arranged by size:
5204 3312 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2446 3670 3689 5292 5720 2446
Size: 1224 19 1603 428 6819
Fragments arranged by size:
6819 1603 1224 428 19
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10093
PsiI TTA'TAA
Cuts at: 7663 7663
Size: 10093
PspOMI G'GGCC_C
Cuts at: 2153 2837 3528 3534 5041 5058 5409 6042 6289
Size: 684 691 6 1507 17 351 633 247
Cuts at: 6289 2153
Size: 5957
Fragments arranged by size:
5957 1507 691 684 633 351 247 17
6
PstI C_TGCA'G
Cuts at: 830 2564 2644 4069 5089 6190 7080 7104 7445
Size: 1734 80 1425 1020 1101 890 24 341
Cuts at: 7445 830
Size: 3478
Fragments arranged by size:
3478 1734 1425 1101 1020 890 341 80
24
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10093
PvuII CAG'CTG
Cuts at: 2565 2698 3346 3620 3707 5615 2565
Size: 133 648 274 87 1908 7043
Fragments arranged by size:
7043 1908 648 274 133 87
RsrII CG'GwC_CG
Cuts at: 2062 2062
Size: 10093
SacI G_AGCT'C
Cuts at: 2733 2821 2733
Size: 88 10005
SalI G'TCGA_C
Cuts at: 1936 7435 1936
Size: 5499 4594
SanDI GG'GwC_CC
Cuts at: 3670 3689 5292 3670
Size: 19 1603 8471
Fragments arranged by size:
8471 1603 19
SapI GCTCTTCn'nnn_
Cuts at: 2429 3422 5464 2429
Size: 993 2042 7058
Fragments arranged by size:
7058 2042 993
SbfI CC_TGCA'GG
Cuts at: 7445 7445
Size: 10093
ScaI AGT'ACT
Cuts at: 2860 8341 10053 2860
Size: 5481 1712 2900
Fragments arranged by size:
5481 2900 1712
SfcI C'TryA_G
Cuts at: 826 1045 2315 2478 2560 2640 2684 3493 4017
Size: 219 1270 163 82 80 44 809 524
Cuts at: 4017 4065 4258 4496 5085 6186 6226 6437 6454
Size: 48 193 238 589 1101 40 211 17
Cuts at: 6454 7076 7100 7316 7441 8576 9257 9448 826
Size: 622 24 216 125 1135 681 191 1471
Fragments arranged by size:
1471 1270 1135 1101 809 681 622 589
524 238 219 216 211 193 191 163
125 82 80 48 44 40 24 17
SfiI GGCCn_nnn'nGGCC
Cuts at: 3972 3972
Size: 10093
SfoI GGC'GCC
Cuts at: 3326 3326
Size: 10093
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10093
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10093
SmaI CCC'GGG
Cuts at: 1872 3533 3587 5528 6384 7420 1872
Size: 1661 54 1941 856 1036 4545
Fragments arranged by size:
4545 1941 1661 1036 856 54
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4325 4571 4916 5380 6715 7325
Size: 197 926 2382 246 345 464 1335 610
Cuts at: 7325 7370 8197 9098 9360 820
Size: 45 827 901 262 1553
Fragments arranged by size:
2382 1553 1335 926 901 827 610 464
345 262 246 197 45
SnaBI TAC'GTA
Cuts at: 493 3173 7884 493
Size: 2680 4711 2702
Fragments arranged by size:
4711 2702 2680
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10093
SphI G_CATG'C
Cuts at: 1132 2434 4250 6878 7451 1132
Size: 1302 1816 2628 573 3774
Fragments arranged by size:
3774 2628 1816 1302 573
SrfI GCCC'GGGC
Cuts at: 3533 5528 3533
Size: 1995 8098
SspI AAT'ATT
Cuts at: 5 52 8017 5
Size: 47 7965 2081
Fragments arranged by size:
7965 2081 47
StuI AGG'CCT
Cuts at: 1517 3446 3470 4271 4504 4523 6089 1517
Size: 1929 24 801 233 19 1566 5521
Fragments arranged by size:
5521 1929 1566 801 233 24 19
StyI C'CwwG_G
Cuts at: 513 1065 2004 3630 3759 4347 4488 4518 4524
Size: 552 939 1626 129 588 141 30 6
Cuts at: 4524 4794 5219 5770 5850 6574 6648 6754 7050
Size: 270 425 551 80 724 74 106 296
Cuts at: 7050 7287 7344 7532 513
Size: 237 57 188 3074
Fragments arranged by size:
3074 1626 939 724 588 552 551 425
296 270 237 188 141 129 106 80
74 57 30 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2612 3650 3758 4378 4959 6492 6670
Size: 1255 1032 1038 108 620 581 1533 178
Cuts at: 6670 8120 8137 8290 8475 8920 325
Size: 1450 17 153 185 445 1498
Fragments arranged by size:
1533 1498 1450 1255 1038 1032 620 581
445 185 178 153 108 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2858 6664 7191
Size: 274 80 33 51 1443 881 3806 527
Cuts at: 7191 8339 10051 96
Size: 1148 1712 138
Fragments arranged by size:
3806 1712 1443 1148 881 527 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4633 4665 6652 6684 10053 10085 4633
Size: 32 1987 32 3369 32 4641
Fragments arranged by size:
4641 3369 1987 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 10093
XbaI T'CTAG_A
Cuts at: 7429 7429
Size: 10093
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3042 4121 4734 3042
Size: 1079 613 8401
Fragments arranged by size:
8401 1079 613
XhoI C'TCGA_G
Cuts at: 1943 7370 1943
Size: 5427 4666
XmaI C'CCGG_G
Cuts at: 1870 3531 3585 5526 6382 7418 1870
Size: 1661 54 1941 856 1036 4545
Fragments arranged by size:
4545 1941 1661 1036 856 54
XmnI GAAnn'nnTTC
Cuts at: 8222 8222
Size: 10093
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7901 276
Size: 53 83 186 936 6367 2468
Fragments arranged by size:
6367 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BstZ17I EcoRV FseI NaeI NgoMIV NruI
PacI PmlI PspXI PsrI SacII SexAI SwaI