Restriction map for
pFN21AA0911
(Circular) (Six-base) MAPSORT of: pFN21AA0911.seq Check: 6030 from: 1 to: 7648
pFN21AA0911 7648 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2792 3569 3914 4332 2792
Size: 777 345 418 6108
Fragments arranged by size:
6108 777 418 345
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5458 278
Size: 53 83 186 936 3922 2468
Fragments arranged by size:
3922 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3680 4991 1937
Size: 1743 1311 4594
Fragments arranged by size:
4594 1743 1311
Acc65I G'GTAC_C
Cuts at: 4969 4969
Size: 7648
AclI AA'CG_TT
Cuts at: 1563 5775 6148 1563
Size: 4212 373 3063
Fragments arranged by size:
4212 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4107 5711 7095 7363 1632
Size: 2475 1604 1384 268 1917
Fragments arranged by size:
2475 1917 1604 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7451
AflIII A'CryG_T
Cuts at: 1203 2876 6547 7524 1203
Size: 1673 3671 977 1327
Fragments arranged by size:
3671 1673 1327 977
AgeI A'CCGG_T
Cuts at: 6479 6479
Size: 7648
AhdI GACnn_n'nnGTC
Cuts at: 6377 6377
Size: 7648
AleI CACnn'nnGTG
Cuts at: 1166 2426 4286 4773 1166
Size: 1260 1860 487 4041
Fragments arranged by size:
4041 1860 1260 487
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3749 1184
Size: 2565 5083
AlwNI CAG_nnn'CTG
Cuts at: 4007 4918 5391 6963 4007
Size: 911 473 1572 4692
Fragments arranged by size:
4692 1572 911 473
ApaLI G'TGCA_C
Cuts at: 4145 4499 5705 6861 4145
Size: 354 1206 1156 4932
Fragments arranged by size:
4932 1206 1156 354
ApoI r'AATT_y
Cuts at: 1493 2268 3401 3855 4010 4418 4957 5183 5335
Size: 775 1133 454 155 408 539 226 152
Cuts at: 5335 7444 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1133 775 539 454 408 226
155 152
AseI AT'TA_AT
Cuts at: 160 1032 6202 160
Size: 872 5170 1606
Fragments arranged by size:
5170 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7648
AvaI C'yCGr_G
Cuts at: 1870 1943 3025 3406 3788 3953 3996 4973 1870
Size: 73 1082 381 382 165 43 977 4545
Fragments arranged by size:
4545 1082 977 382 381 165 73 43
BamHI G'GATC_C
Cuts at: 3032 3120 3304 4647 4978 5345 3032
Size: 88 184 1343 331 367 5335
Fragments arranged by size:
5335 1343 367 331 184 88
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2420 2834 3104 3249 3276
Size: 325 217 627 633 414 270 145 27
Cuts at: 3276 3378 4969 6424 618
Size: 102 1591 1455 1842
Fragments arranged by size:
1842 1591 1455 633 627 414 325 270
217 145 102 27
BanII G_rGCy'C
Cuts at: 1391 2124 3447 3963 4967 1391
Size: 733 1323 516 1004 4072
Fragments arranged by size:
4072 1323 1004 733 516
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3723 3789 928
Size: 971 1824 66 4787
Fragments arranged by size:
4787 1824 971 66
BbvCI CC'TCA_GC
Cuts at: 4101 4940 4101
Size: 839 6809
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5839 5873 1903
Size: 34 50 34 3818 34 3678
Fragments arranged by size:
3818 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4150 5540 6750 4150
Size: 1390 1210 5048
Fragments arranged by size:
5048 1390 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7648
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3978 6259 136
Size: 107 122 71 631 605 2306 2281 1525
Fragments arranged by size:
2306 2281 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7643 7643
Size: 7648
BlpI GC'TnA_GC
Cuts at: 5065 5065
Size: 7648
Bme1580I G_kGCm'C
Cuts at: 1790 2634 2837 3005 4149 4503 5709 6865 1790
Size: 844 203 168 1144 354 1206 1156 2573
Fragments arranged by size:
2573 1206 1156 1144 844 354 203 168
BmgBI CAC'GTC
Cuts at: 1318 2473 2629 3435 1318
Size: 1155 156 806 5531
Fragments arranged by size:
5531 1155 806 156
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3595 4277 4720
Size: 416 53 83 399 24 2178 682 443
Cuts at: 4720 5379 6337 442
Size: 659 958 1753
Fragments arranged by size:
2178 1753 958 682 659 443 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7648
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3432 3466 3803 3432
Size: 34 337 7277
Fragments arranged by size:
7277 337 34
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2138 2607 2891 4232 4940 6308 1239
Size: 899 469 284 1341 708 1368 2579
Fragments arranged by size:
2579 1368 1341 899 708 469 284
Bpu10I CC'TnA_GC
Cuts at: 4044 4101 4940 4044
Size: 57 839 6752
Fragments arranged by size:
6752 839 57
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5773 6638 6936 5773
Size: 865 298 6485
Fragments arranged by size:
6485 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4745 6311 882
Size: 617 3246 1566 2219
Fragments arranged by size:
3246 2219 1566 617
BsaAI yAC'GTr
Cuts at: 493 1204 3937 5439 493
Size: 711 2733 1502 2702
Fragments arranged by size:
2733 2702 1502 711
BsaBI GATnn'nnATC
Cuts at: 3243 7642 3243
Size: 4399 3249
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3132 3981 5455 5837
Size: 53 83 186 936 1599 849 1474 382
Cuts at: 5837 6472 275
Size: 635 1451
Fragments arranged by size:
1599 1474 1451 936 849 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4365 6081 6479 6753 6900 7311 4365
Size: 1716 398 274 147 411 4702
Fragments arranged by size:
4702 1716 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3242 3272 3815 3845 1178
Size: 30 165 30 1839 30 543 30 4981
Fragments arranged by size:
4981 1839 543 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2156 3329 3971 4151 4199 4325 4793 4796
Size: 970 1173 642 180 48 126 468 3
Cuts at: 4796 4799 4880 4883 4886 1186
Size: 3 81 3 3 3948
Fragments arranged by size:
3948 1173 970 642 468 180 126 81
48 3 3 3 3
BseYI C'CCAG_C
Cuts at: 3752 4485 4574 6851 7347 3752
Size: 733 89 2277 496 4053
Fragments arranged by size:
4053 2277 733 496 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2770 3575 3755 1163
Size: 573 1034 805 180 5056
Fragments arranged by size:
5056 1034 805 573 180
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2040 2950 2997 5098 5859 6887
Size: 275 186 39 910 47 2101 761 1028
Cuts at: 6887 1540
Size: 2301
Fragments arranged by size:
2301 2101 1028 910 761 275 186 47
39
BsiHKAI G_wGCw'C
Cuts at: 2166 4149 4503 5709 5794 6865 2166
Size: 1983 354 1206 85 1071 2949
Fragments arranged by size:
2949 1983 1206 1071 354 85
BsmI GAATG_Cn'
Cuts at: 5159 5252 5159
Size: 93 7555
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 2166 2634 2837 3005 3447 3963
Size: 399 334 42 468 203 168 442 516
Cuts at: 3963 4149 4503 4967 5709 5794 6865 1391
Size: 186 354 464 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 516 468 464 442 399
354 334 203 186 168 85 42
BspEI T'CCGG_A
Cuts at: 7311 7311
Size: 7648
BspHI T'CATG_A
Cuts at: 4623 5537 7267 4623
Size: 914 1730 5004
Fragments arranged by size:
5004 1730 914
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2792 3340 3569 3597 3692 3697 3711 3914
Size: 1948 548 229 28 95 5 14 203
Cuts at: 3914 4332 5003 844
Size: 418 671 3489
Fragments arranged by size:
3489 1948 671 548 418 229 203 95
28 14 5
BsrBI CCG'CTC
Cuts at: 1394 1823 2021 3472 4515 5535 1394
Size: 429 198 1451 1043 1020 3507
Fragments arranged by size:
3507 1451 1043 1020 429 198
BsrDI GCAATG_nn'
Cuts at: 65 1248 6143 6317 65
Size: 1183 4895 174 1396
Fragments arranged by size:
4895 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2837 2979 3279 6292 6479 1700
Size: 110 1027 142 300 3013 187 2869
Fragments arranged by size:
3013 2869 1027 300 187 142 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7648
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7648
BssSI C'ACGA_G
Cuts at: 3440 3500 4069 4196 5708 6720 3440
Size: 60 569 127 1512 1012 4368
Fragments arranged by size:
4368 1512 1012 569 127 60
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4327 794
Size: 3533 4115
BstBI TT'CG_AA
Cuts at: 6452 6452
Size: 7648
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7648
BstXI CCAn_nnnn'nTGG
Cuts at: 3760 4459 4477 3760
Size: 699 18 6931
Fragments arranged by size:
6931 699 18
BstYI r'GATC_y
Cuts at: 1972 3032 3120 3304 3738 4647 4978 5345 5730
Size: 1060 88 184 434 909 331 367 385
Cuts at: 5730 5747 7199 7285 7297 7643 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1060 909 434 385 367 346
331 184 88 86 17 12
BstZ17I GTA'TAC
Cuts at: 3681 3681
Size: 7648
BtgI C'CryG_G
Cuts at: 513 1065 2131 2517 2565 2997 3048 3271 4296
Size: 552 1066 386 48 432 51 223 1025
Cuts at: 4296 4458 4746 513
Size: 162 288 3415
Fragments arranged by size:
3415 1066 1025 552 432 386 288 223
162 51 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6909
Fragments arranged by size:
6909 683 56
BtsI GCAGTG_nn'
Cuts at: 2304 2715 3003 3120 4578 4780 5164 5958 5978
Size: 411 288 117 1458 202 384 794 20
Cuts at: 5978 2304
Size: 3974
Fragments arranged by size:
3974 1458 794 411 384 288 202 117
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7648
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7613
DraI TTT'AAA
Cuts at: 2266 4952 2266
Size: 2686 4962
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2888 3707 6655 809
Size: 507 1572 819 2948 1802
Fragments arranged by size:
2948 1802 1572 819 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2055 2847
Size: 54 1425 50 275 18 207 18 792
Cuts at: 2847 2994 3070 4299 4399 4449 4545 5095 5984
Size: 147 76 1229 100 50 96 550 889
Cuts at: 5984 7613 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1229 889 792 550 275 207
147 100 96 76 54 50 50 43
18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2037 2994 5095 1537
Size: 275 225 957 2101 4090
Fragments arranged by size:
4090 2101 957 275 225
EarI CTCTTCn'nnn_
Cuts at: 1353 3299 4785 4791 4797 4821 5578 1353
Size: 1946 1486 6 6 24 757 3423
Fragments arranged by size:
3423 1946 1486 757 24 6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4681 6235 6609 6755 387
Size: 1064 138 3092 1554 374 146 1280
Fragments arranged by size:
3092 1554 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2138 2607 2891 4107 4232 4940 5711
Size: 393 506 469 284 1216 125 708 771
Cuts at: 5711 6308 7095 7363 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1216 787 771 708 597 506 469
393 284 268 125
EcoO109I rG'GnC_Cy
Cuts at: 2411 3709 4105 4353 2411
Size: 1298 396 248 5706
Fragments arranged by size:
5706 1298 396 248
EcoRI G'AATT_C
Cuts at: 3401 3855 4957 5335 3401
Size: 454 1102 378 5714
Fragments arranged by size:
5714 1102 454 378
FspI TGC'GCA
Cuts at: 6154 6154
Size: 7648
HaeII r_GCGC'y
Cuts at: 1678 4848 6795 1678
Size: 3170 1947 2531
Fragments arranged by size:
3170 2531 1947
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2945 2977 3419 3451 3478
Size: 32 135 32 1373 32 442 32 27
Cuts at: 3478 3510 3658 3690 3855 3887 6289 6321 6363
Size: 32 148 32 165 32 2402 32 42
Cuts at: 6363 6395 1373
Size: 32 2626
Fragments arranged by size:
2626 2402 1373 442 165 148 135 42
32 32 32 32 32 32 32 32
32 27
HincII GTy'rAC
Cuts at: 669 1657 1938 2098 2644 4525 4741 4992 5238
Size: 988 281 160 546 1881 216 251 246
Cuts at: 5238 669
Size: 3079
Fragments arranged by size:
3079 1881 988 546 281 251 246 216
160
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7648
HpaI GTT'AAC
Cuts at: 2098 5238 2098
Size: 3140 4508
KpnI G_GTAC'C
Cuts at: 4973 4973
Size: 7648
MfeI C'AATT_G
Cuts at: 5247 5247
Size: 7648
MluI A'CGCG_T
Cuts at: 7524 7524
Size: 7648
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4833 6762 6946 1305
Size: 3528 1929 184 2007
Fragments arranged by size:
3528 2007 1929 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3072 4301 4547 10
Size: 54 1425 343 1240 1229 246 3111
Fragments arranged by size:
3111 1425 1240 1229 343 246 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2426 3170 4250 4286 4773 5606 5965
Size: 648 1260 744 1080 36 487 833 359
Cuts at: 5965 6124 518
Size: 159 2042
Fragments arranged by size:
2042 1260 1080 833 744 648 487 359
159 36
MspA1I CmG'CkG
Cuts at: 1606 2999 4918 5064 5741 6889 7134 1606
Size: 1393 1919 146 677 1148 245 2120
Fragments arranged by size:
2120 1919 1393 1148 677 245 146
NaeI GCC'GGC
Cuts at: 3281 3281
Size: 7648
NcoI C'CATG_G
Cuts at: 513 1065 3048 4296 4458 4746 513
Size: 552 1983 1248 162 288 3415
Fragments arranged by size:
3415 1983 1248 552 288 162
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7648
NgoMIV G'CCGG_C
Cuts at: 3279 3279
Size: 7648
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7648
NotI GC'GGCC_GC
Cuts at: 2994 5095 2994
Size: 2101 5547
NspI r_CATG'y
Cuts at: 1132 1229 2880 4349 5006 6551 1132
Size: 97 1651 1469 657 1545 2229
Fragments arranged by size:
2229 1651 1545 1469 657 97
PasI CC'CwG_GG
Cuts at: 1348 2792 1348
Size: 1444 6204
PciI A'CATG_T
Cuts at: 2876 6547 2876
Size: 3671 3977
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4543 1108
Size: 3435 4213
PfoI T'CCnGG_A
Cuts at: 3123 3641 3123
Size: 518 7130
PmeI GTTT'AAAC
Cuts at: 4952 4952
Size: 7648
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5717 5749 7230 7262 5717
Size: 32 1481 32 6103
Fragments arranged by size:
6103 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2411 3709 4105 4353 2411
Size: 1298 396 248 5706
Fragments arranged by size:
5706 1298 396 248
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7648
PsiI TTA'TAA
Cuts at: 5218 5218
Size: 7648
PstI C_TGCA'G
Cuts at: 830 2367 3337 3580 3708 4236 5000 830
Size: 1537 970 243 128 528 764 3478
Fragments arranged by size:
3478 1537 970 764 528 243 128
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7648
PvuII CAG'CTG
Cuts at: 4918 4918
Size: 7648
SacII CC_GC'GG
Cuts at: 3000 3000
Size: 7648
SalI G'TCGA_C
Cuts at: 1936 4990 1936
Size: 3054 4594
SbfI CC_TGCA'GG
Cuts at: 3708 5000 3708
Size: 1292 6356
ScaI AGT'ACT
Cuts at: 5896 7608 5896
Size: 1712 5936
SexAI A'CCwGG_T
Cuts at: 3084 3084
Size: 7648
SfcI C'TryA_G
Cuts at: 826 1045 2363 3333 3576 3704 4232 4996 6131
Size: 219 1318 970 243 128 528 764 1135
Cuts at: 6131 6812 7003 826
Size: 681 191 1471
Fragments arranged by size:
1471 1318 1135 970 764 681 528 243
219 191 128
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7648
SgrAI Cr'CCGG_yG
Cuts at: 1700 2837 1700
Size: 1137 6511
SmaI CCC'GGG
Cuts at: 1872 4975 1872
Size: 3103 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5752 6653 6915 820
Size: 197 926 3809 901 262 1553
Fragments arranged by size:
3809 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5439 493
Size: 4946 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7648
SphI G_CATG'C
Cuts at: 1132 4349 5006 1132
Size: 3217 657 3774
Fragments arranged by size:
3774 3217 657
SspI AAT'ATT
Cuts at: 5 52 5572 5
Size: 47 5520 2081
Fragments arranged by size:
5520 2081 47
StuI AGG'CCT
Cuts at: 1517 2393 1517
Size: 876 6772
StyI C'CwwG_G
Cuts at: 513 1065 3048 3771 4296 4458 4746 5087 513
Size: 552 1983 723 525 162 288 341 3074
Fragments arranged by size:
3074 1983 723 552 525 341 288 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2784 2964 3122 3130 3904 5675 5692
Size: 1255 1204 180 158 8 774 1771 17
Cuts at: 5692 5845 6030 6475 325
Size: 153 185 445 1498
Fragments arranged by size:
1771 1498 1255 1204 774 445 185 180
158 153 17 8
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2899 5894 7606
Size: 274 80 33 51 1443 922 2995 1712
Cuts at: 7606 96
Size: 138
Fragments arranged by size:
2995 1712 1443 922 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3242 3274 7608 7640 3242
Size: 32 4334 32 3250
Fragments arranged by size:
4334 3250 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7648
XbaI T'CTAG_A
Cuts at: 4984 4984
Size: 7648
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2347 2984 2347
Size: 637 7011
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7648
XmaI C'CCGG_G
Cuts at: 1870 4973 1870
Size: 3103 4545
XmnI GAAnn'nnTTC
Cuts at: 5777 5777
Size: 7648
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5456 276
Size: 53 83 186 936 3922 2468
Fragments arranged by size:
3922 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacI SanDI SapI SfiI
SfoI SrfI SwaI