Restriction map for
pFN21AA0910
(Circular) (Six-base) MAPSORT of: pFN21AA0910.seq Check: 4999 from: 1 to: 8542
pFN21AA0910 8542 bp
With 182 enzymes: SgfI *
March 12, 2010 11:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2851 2851
Size: 8542
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6352 278
Size: 53 83 186 936 4816 2468
Fragments arranged by size:
4816 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5885 1937
Size: 3948 4594
Acc65I G'GTAC_C
Cuts at: 5863 5863
Size: 8542
AclI AA'CG_TT
Cuts at: 1563 6669 7042 1563
Size: 5106 373 3063
Fragments arranged by size:
5106 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2304 2319 3833 4488 4586 4650 5085 5328
Size: 672 15 1514 655 98 64 435 243
Cuts at: 5328 5651 5784 6605 7989 8257 1632
Size: 323 133 821 1384 268 1917
Fragments arranged by size:
1917 1514 1384 821 672 655 435 323
268 243 133 98 64 15
AflII C'TTAA_G
Cuts at: 820 1017 2870 820
Size: 197 1853 6492
Fragments arranged by size:
6492 1853 197
AflIII A'CryG_T
Cuts at: 1203 4521 7441 8418 1203
Size: 3318 2920 977 1327
Fragments arranged by size:
3318 2920 1327 977
AgeI A'CCGG_T
Cuts at: 7373 7373
Size: 8542
AhdI GACnn_n'nnGTC
Cuts at: 7271 7271
Size: 8542
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8542
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4559 4785 4988 1184
Size: 3375 226 203 4738
Fragments arranged by size:
4738 3375 226 203
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3099 3131 3099
Size: 32 8510
AlwNI CAG_nnn'CTG
Cuts at: 3332 5471 6285 7857 3332
Size: 2139 814 1572 4017
Fragments arranged by size:
4017 2139 1572 814
ApaLI G'TGCA_C
Cuts at: 5244 6599 7755 5244
Size: 1355 1156 6031
Fragments arranged by size:
6031 1355 1156
ApoI r'AATT_y
Cuts at: 1493 2271 2374 2902 2998 3571 3600 4512 4930
Size: 778 103 528 96 573 29 912 418
Cuts at: 4930 5378 5743 5851 6077 6229 8338 1493
Size: 448 365 108 226 152 2109 1697
Fragments arranged by size:
2109 1697 912 778 573 528 448 418
365 226 152 108 103 96 29
AseI AT'TA_AT
Cuts at: 160 1032 7096 160
Size: 872 6064 1606
Fragments arranged by size:
6064 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8542
AvaI C'yCGr_G
Cuts at: 1870 1943 3483 5867 1870
Size: 73 1540 2384 4545
Fragments arranged by size:
4545 2384 1540 73
BamHI G'GATC_C
Cuts at: 2044 5872 6239 2044
Size: 3828 367 4347
Fragments arranged by size:
4347 3828 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5863 7318 618
Size: 325 217 627 4076 1455 1842
Fragments arranged by size:
4076 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3340 4239 5861 1391
Size: 1949 899 1622 4072
Fragments arranged by size:
4072 1949 1622 899
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3860 3986 4574 5566 5652 928
Size: 971 1961 126 588 992 86 3818
Fragments arranged by size:
3818 1961 992 971 588 126 86
BbvCI CC'TCA_GC
Cuts at: 5085 5085
Size: 8542
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3132 3166 6733 6767 1903
Size: 34 1195 34 3567 34 3678
Fragments arranged by size:
3678 3567 1195 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5471 6434 7644 5471
Size: 963 1210 6369
Fragments arranged by size:
6369 1210 963
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8542
BfrBI ATG'CAT
Cuts at: 2180 2180
Size: 8542
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7153 136
Size: 107 122 71 631 605 5481 1525
Fragments arranged by size:
5481 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4471 4477 8537 4471
Size: 6 4060 4476
Fragments arranged by size:
4476 4060 6
BlpI GC'TnA_GC
Cuts at: 4560 5959 4560
Size: 1399 7143
Bme1580I G_kGCm'C
Cuts at: 1790 5248 5530 6603 7759 1790
Size: 3458 282 1073 1156 2573
Fragments arranged by size:
3458 2573 1156 1073 282
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8542
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3455 5235 6273
Size: 416 53 83 399 24 2038 1780 1038
Cuts at: 6273 7231 442
Size: 958 1753
Fragments arranged by size:
2038 1780 1753 1038 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3212 1056
Size: 2156 6386
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4858 5352 4858
Size: 494 8048
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3354 3847 4515 5078 5535 7202 1239
Size: 2115 493 668 563 457 1667 2579
Fragments arranged by size:
2579 2115 1667 668 563 493 457
Bpu10I CC'TnA_GC
Cuts at: 5085 5085
Size: 8542
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3048 5430 6667 7532 7830 3048
Size: 2382 1237 865 298 3760
Fragments arranged by size:
3760 2382 1237 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4702 5323 7205 882
Size: 617 3203 621 1882 2219
Fragments arranged by size:
3203 2219 1882 621 617
BsaAI yAC'GTr
Cuts at: 493 1204 4524 6333 493
Size: 711 3320 1809 2702
Fragments arranged by size:
3320 2702 1809 711
BsaBI GATnn'nnATC
Cuts at: 2343 4476 8536 2343
Size: 2133 4060 2349
Fragments arranged by size:
4060 2349 2133
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3909 6349 6731 7366
Size: 53 83 186 936 2376 2440 382 635
Cuts at: 7366 275
Size: 1451
Fragments arranged by size:
2440 2376 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2025 6975 7373 7647 7794 8205 2025
Size: 4950 398 274 147 411 2362
Fragments arranged by size:
4950 2362 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4330 4360 4513 4543 5488
Size: 30 165 30 2927 30 153 30 945
Cuts at: 5488 5518 1178
Size: 30 4202
Fragments arranged by size:
4202 2927 945 165 153 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2574 5325 5496 1186
Size: 1388 2751 171 4232
Fragments arranged by size:
4232 2751 1388 171
BseYI C'CCAG_C
Cuts at: 2639 5129 5736 7745 8241 2639
Size: 2490 607 2009 496 2940
Fragments arranged by size:
2940 2490 2009 607 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3203 4529 5346 1163
Size: 573 1467 1326 817 4359
Fragments arranged by size:
4359 1467 1326 817 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5298 5992 6753 7781 1540
Size: 275 186 3297 694 761 1028 2301
Fragments arranged by size:
3297 2301 1028 761 694 275 186
BsiHKAI G_wGCw'C
Cuts at: 2127 3340 3373 3490 4239 5218 5248 5389 6603
Size: 1213 33 117 749 979 30 141 1214
Cuts at: 6603 6688 7759 2127
Size: 85 1071 2910
Fragments arranged by size:
2910 1214 1213 1071 979 749 141 117
85 33 30
BsmI GAATG_Cn'
Cuts at: 3796 4035 6053 6146 3796
Size: 239 2018 93 6192
Fragments arranged by size:
6192 2018 239 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2127 3340 3373 3490 4239 5218 5248
Size: 399 337 1213 33 117 749 979 30
Cuts at: 5248 5389 5530 5861 6603 6688 7759 1391
Size: 141 141 331 742 85 1071 2174
Fragments arranged by size:
2174 1213 1071 979 749 742 399 337
331 141 141 117 85 33 30
BspEI T'CCGG_A
Cuts at: 2025 8205 2025
Size: 6180 2362
BspHI T'CATG_A
Cuts at: 6431 8161 6431
Size: 1730 6812
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2851 5479 5769 5897 844
Size: 2007 2628 290 128 3489
Fragments arranged by size:
3489 2628 2007 290 128
BsrBI CCG'CTC
Cuts at: 1394 1823 6429 1394
Size: 429 4606 3507
Fragments arranged by size:
4606 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4965 4970 7037 7211 65
Size: 1183 3717 5 2067 174 1396
Fragments arranged by size:
3717 2067 1396 1183 174 5
BsrFI r'CCGG_y
Cuts at: 1700 1810 7186 7373 1700
Size: 110 5376 187 2869
Fragments arranged by size:
5376 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4518 96
Size: 4422 4120
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8542
BssSI C'ACGA_G
Cuts at: 5508 6602 7614 5508
Size: 1094 1012 6436
Fragments arranged by size:
6436 1094 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2402 5018 5456 794
Size: 1608 2616 438 3880
Fragments arranged by size:
3880 2616 1608 438
BstBI TT'CG_AA
Cuts at: 3142 7346 3142
Size: 4204 4338
BstEII G'GTnAC_C
Cuts at: 1184 4336 1184
Size: 3152 5390
BstXI CCAn_nnnn'nTGG
Cuts at: 2173 2772 2173
Size: 599 7943
BstYI r'GATC_y
Cuts at: 1972 2028 2044 2756 2798 2912 4471 4477 5872
Size: 56 16 712 42 114 1559 6 1395
Cuts at: 5872 6239 6624 6641 8093 8179 8191 8537 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1559 1452 1395 712 385 367 346
114 86 56 42 17 16 12 6
Bsu36I CC'TnA_GG
Cuts at: 5637 5637
Size: 8542
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 7051
Fragments arranged by size:
7051 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7803
Fragments arranged by size:
7803 683 56
BtsI GCAGTG_nn'
Cuts at: 2318 6058 6852 6872 2318
Size: 3740 794 20 3988
Fragments arranged by size:
3988 3740 794 20
ClaI AT'CG_AT
Cuts at: 1554 2328 4209 1554
Size: 774 1881 5887
Fragments arranged by size:
5887 1881 774
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8507
DraI TTT'AAA
Cuts at: 4908 5846 4908
Size: 938 7604
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3532 7549 809
Size: 507 2216 4017 1802
Fragments arranged by size:
4017 2216 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5989 6878 8507
Size: 54 1425 50 275 18 4159 889 1629
Cuts at: 8507 8
Size: 43
Fragments arranged by size:
4159 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5989 1537
Size: 275 4177 4090
Fragments arranged by size:
4177 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4040 4549 4626 5140 6472 1353
Size: 2687 509 77 514 1332 3423
Fragments arranged by size:
3423 2687 1332 514 509 77
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5328 7129 7503 7649 387
Size: 1064 138 3739 1801 374 146 1280
Fragments arranged by size:
3739 1801 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3338 4237 3338
Size: 899 7643
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2304 2319 3354 3833 3847 4488 4515
Size: 393 672 15 1035 479 14 641 27
Cuts at: 4515 4586 4650 5078 5085 5328 5535 5651 5784
Size: 71 64 428 7 243 207 116 133
Cuts at: 5784 6605 7202 7989 8257 1239
Size: 821 597 787 268 1524
Fragments arranged by size:
1524 1035 821 787 672 641 597 479
428 393 268 243 207 133 116 71
64 27 15 14 7
EcoNI CCTnn'n_nnAGG
Cuts at: 5448 5448
Size: 8542
EcoO109I rG'GnC_Cy
Cuts at: 5180 5468 5180
Size: 288 8254
EcoRI G'AATT_C
Cuts at: 2374 5851 6229 2374
Size: 3477 378 4687
Fragments arranged by size:
4687 3477 378
EcoRV GAT'ATC
Cuts at: 2986 2986
Size: 8542
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2961 4357 4381 4808 5471 5714 2961
Size: 1396 24 427 663 243 5789
Fragments arranged by size:
5789 1396 663 427 243 24
FspI TGC'GCA
Cuts at: 2439 7048 2439
Size: 4609 3933
FspAI rTGC'GCAy
Cuts at: 2439 2439
Size: 8542
HaeII r_GCGC'y
Cuts at: 1678 7689 1678
Size: 6011 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2013 2045 2300 2332 2553
Size: 32 135 32 441 32 255 32 221
Cuts at: 2553 2585 3597 3629 5488 5491 5520 5523 5819
Size: 32 1012 32 1859 3 29 3 296
Cuts at: 5819 5851 7183 7215 7257 7289 1373
Size: 32 1332 32 42 32 2626
Fragments arranged by size:
2626 1859 1332 1012 441 296 255 221
135 42 32 32 32 32 32 32
32 32 32 29 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 5886 6132 669
Size: 988 281 3948 246 3079
Fragments arranged by size:
3948 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2943 2964 5805 748
Size: 2195 21 2841 3485
Fragments arranged by size:
3485 2841 2195 21
HpaI GTT'AAC
Cuts at: 6132 6132
Size: 8542
KpnI G_GTAC'C
Cuts at: 5867 5867
Size: 8542
MfeI C'AATT_G
Cuts at: 3762 6141 3762
Size: 2379 6163
MluI A'CGCG_T
Cuts at: 8418 8418
Size: 8542
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2245 3201 3595 4373 7656 7840 1305
Size: 940 956 394 778 3283 184 2007
Fragments arranged by size:
3283 2007 956 940 778 394 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6720
Fragments arranged by size:
6720 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4173 6500 6859 7018 518
Size: 648 3007 2327 359 159 2042
Fragments arranged by size:
3007 2327 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4466 5958 6635 7783 8028 1606
Size: 2860 1492 677 1148 245 2120
Fragments arranged by size:
2860 2120 1492 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 7051
Fragments arranged by size:
7051 939 552
NdeI CA'TA_TG
Cuts at: 387 2182 2931 387
Size: 1795 749 5998
Fragments arranged by size:
5998 1795 749
NheI G'CTAG_C
Cuts at: 1052 3208 1052
Size: 2156 6386
NotI GC'GGCC_GC
Cuts at: 5989 5989
Size: 8542
NsiI A_TGCA'T
Cuts at: 2182 2182
Size: 8542
NspI r_CATG'y
Cuts at: 1132 1229 2452 2978 3565 3944 5900 7445 1132
Size: 97 1223 526 587 379 1956 1545 2229
Fragments arranged by size:
2229 1956 1545 1223 587 526 379 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8542
PciI A'CATG_T
Cuts at: 7441 7441
Size: 8542
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4682 1108
Size: 3574 4968
PfoI T'CCnGG_A
Cuts at: 3717 3717
Size: 8542
PmeI GTTT'AAAC
Cuts at: 5846 5846
Size: 8542
PmlI CAC'GTG
Cuts at: 4524 4524
Size: 8542
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2745 2777 3322 3354 6611 6643 8124 8156 2745
Size: 32 545 32 3257 32 1481 32 3131
Fragments arranged by size:
3257 3131 1481 545 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 5180 5468 5180
Size: 288 8254
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8542
PsiI TTA'TAA
Cuts at: 4650 4796 6112 4650
Size: 146 1316 7080
Fragments arranged by size:
7080 1316 146
PspXI vC'TCGA_Gb
Cuts at: 3483 3483
Size: 8542
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4650 4682 4650
Size: 32 8510
PstI C_TGCA'G
Cuts at: 830 2137 4081 4764 5894 830
Size: 1307 1944 683 1130 3478
Fragments arranged by size:
3478 1944 1307 1130 683
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8542
PvuII CAG'CTG
Cuts at: 4466 4466
Size: 8542
SacI G_AGCT'C
Cuts at: 3340 4239 3340
Size: 899 7643
SalI G'TCGA_C
Cuts at: 1936 5884 1936
Size: 3948 4594
SapI GCTCTTCn'nnn_
Cuts at: 4040 4549 5140 4040
Size: 509 591 7442
Fragments arranged by size:
7442 591 509
SbfI CC_TGCA'GG
Cuts at: 5894 5894
Size: 8542
ScaI AGT'ACT
Cuts at: 2172 3552 6790 8502 2172
Size: 1380 3238 1712 2212
Fragments arranged by size:
3238 2212 1712 1380
SexAI A'CCwGG_T
Cuts at: 2645 2645
Size: 8542
SfcI C'TryA_G
Cuts at: 826 1045 2133 3840 4063 4077 4760 5056 5890
Size: 219 1088 1707 223 14 683 296 834
Cuts at: 5890 7025 7706 7897 826
Size: 1135 681 191 1471
Fragments arranged by size:
1707 1471 1135 1088 834 683 681 296
223 219 191 14
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8542
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8542
SmaI CCC'GGG
Cuts at: 1872 5869 1872
Size: 3997 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2870 3063 3483 5445 6646 7547
Size: 197 926 927 193 420 1962 1201 901
Cuts at: 7547 7809 820
Size: 262 1553
Fragments arranged by size:
1962 1553 1201 927 926 901 420 262
197 193
SnaBI TAC'GTA
Cuts at: 493 6333 493
Size: 5840 2702
SpeI A'CTAG_T
Cuts at: 152 5147 152
Size: 4995 3547
SphI G_CATG'C
Cuts at: 1132 2452 3944 5900 1132
Size: 1320 1492 1956 3774
Fragments arranged by size:
3774 1956 1492 1320
SspI AAT'ATT
Cuts at: 5 52 3584 6466 5
Size: 47 3532 2882 2081
Fragments arranged by size:
3532 2882 2081 47
StuI AGG'CCT
Cuts at: 1517 4585 1517
Size: 3068 5474
StyI C'CwwG_G
Cuts at: 513 1065 2004 5116 5981 513
Size: 552 939 3112 865 3074
Fragments arranged by size:
3112 3074 939 865 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3270 3715 5380 5404 5509 6569 6586
Size: 1255 1690 445 1665 24 105 1060 17
Cuts at: 6586 6739 6924 7369 325
Size: 153 185 445 1498
Fragments arranged by size:
1690 1665 1498 1255 1060 445 445 185
153 105 24 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2170 2715 3550
Size: 274 80 33 51 1443 193 545 835
Cuts at: 3550 4518 5483 6788 8500 96
Size: 968 965 1305 1712 138
Fragments arranged by size:
1712 1443 1305 968 965 835 545 274
193 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3789 3821 4408 4440 4513 4545 8502 8534 3789
Size: 32 587 32 73 32 3957 32 3797
Fragments arranged by size:
3957 3797 587 73 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4424 5430 1316
Size: 3108 1006 4428
Fragments arranged by size:
4428 3108 1006
XbaI T'CTAG_A
Cuts at: 3079 4474 5878 3079
Size: 1395 1404 5743
Fragments arranged by size:
5743 1404 1395
XhoI C'TCGA_G
Cuts at: 1943 3483 1943
Size: 1540 7002
XmaI C'CCGG_G
Cuts at: 1870 5867 1870
Size: 3997 4545
XmnI GAAnn'nnTTC
Cuts at: 2329 3276 3401 5079 6671 2329
Size: 947 125 1678 1592 4200
Fragments arranged by size:
4200 1678 1592 947 125
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6350 276
Size: 53 83 186 936 4816 2468
Fragments arranged by size:
4816 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI
BstZ17I DraIII FseI KasI NaeI NarI NgoMIV NruI
PacI PspOMI RsrII SacII SanDI SfiI SfoI SrfI
SwaI XcmI