Restriction map for
pFN21AA0905
(Circular) (Six-base) MAPSORT of: pFN21AA0905.seq Check: 728 from: 1 to: 8365
pFN21AA0905 8365 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2267 3806 2267
Size: 1539 6826
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6175 278
Size: 53 83 186 936 4639 2468
Fragments arranged by size:
4639 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5708 1937
Size: 3771 4594
Acc65I G'GTAC_C
Cuts at: 2208 4606 5686 2208
Size: 2398 1080 4887
Fragments arranged by size:
4887 2398 1080
AclI AA'CG_TT
Cuts at: 1563 5449 6492 6865 1563
Size: 3886 1043 373 3063
Fragments arranged by size:
3886 3063 1043 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3173 3323 3630 4139 4881 5109 5385 5581
Size: 1541 150 307 509 742 228 276 196
Cuts at: 5581 6428 7812 8080 1632
Size: 847 1384 268 1917
Fragments arranged by size:
1917 1541 1384 847 742 509 307 276
268 228 196 150
AfeI AGC'GCT
Cuts at: 5390 5390
Size: 8365
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8168
AflIII A'CryG_T
Cuts at: 1203 7264 8241 1203
Size: 6061 977 1327
Fragments arranged by size:
6061 1327 977
AgeI A'CCGG_T
Cuts at: 2712 7196 2712
Size: 4484 3881
AhdI GACnn_n'nnGTC
Cuts at: 5098 7094 5098
Size: 1996 6369
AleI CACnn'nnGTG
Cuts at: 1166 2917 3896 1166
Size: 1751 979 5635
Fragments arranged by size:
5635 1751 979
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2121 4090 4716 1184
Size: 937 1969 626 4833
Fragments arranged by size:
4833 1969 937 626
AlwNI CAG_nnn'CTG
Cuts at: 2822 4162 4457 6108 7680 2822
Size: 1340 295 1651 1572 3507
Fragments arranged by size:
3507 1651 1572 1340 295
ApaLI G'TGCA_C
Cuts at: 6422 7578 6422
Size: 1156 7209
ApoI r'AATT_y
Cuts at: 1493 3111 3407 3659 3995 4407 5674 5900 6052
Size: 1618 296 252 336 412 1267 226 152
Cuts at: 6052 8161 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1618 1267 412 336 296 252
226 152
AseI AT'TA_AT
Cuts at: 160 1032 6919 160
Size: 872 5887 1606
Fragments arranged by size:
5887 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8365
AvaI C'yCGr_G
Cuts at: 1870 1943 5690 1870
Size: 73 3747 4545
Fragments arranged by size:
4545 3747 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4590 4623 4590
Size: 33 8332
BamHI G'GATC_C
Cuts at: 5695 6062 5695
Size: 367 7998
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2208 4606 5261 5686 7141
Size: 325 217 627 421 2398 655 425 1455
Cuts at: 7141 618
Size: 1842
Fragments arranged by size:
2398 1842 1455 655 627 425 421 325
217
BanII G_rGCy'C
Cuts at: 1391 2051 3602 4885 5275 5684 1391
Size: 660 1551 1283 390 409 4072
Fragments arranged by size:
4072 1551 1283 660 409 390
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5145 928
Size: 971 3246 4148
Fragments arranged by size:
4148 3246 971
BbvCI CC'TCA_GC
Cuts at: 3320 4689 3320
Size: 1369 6996
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6556 6590 1903
Size: 34 4619 34 3678
Fragments arranged by size:
4619 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3479 4662 6257 7467 3479
Size: 1183 1595 1210 4377
Fragments arranged by size:
4377 1595 1210 1183
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8365
BfrBI ATG'CAT
Cuts at: 2647 4540 5569 2647
Size: 1893 1029 5443
Fragments arranged by size:
5443 1893 1029
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2039 3551 5270
Size: 107 122 71 631 605 367 1512 1719
Cuts at: 5270 6976 136
Size: 1706 1525
Fragments arranged by size:
1719 1706 1525 1512 631 605 367 122
107 71
BglII A'GATC_T
Cuts at: 3499 8360 3499
Size: 4861 3504
BlpI GC'TnA_GC
Cuts at: 2094 3129 3258 5782 2094
Size: 1035 129 2524 4677
Fragments arranged by size:
4677 2524 1035 129
Bme1580I G_kGCm'C
Cuts at: 1790 5266 6426 7582 1790
Size: 3476 1160 1156 2573
Fragments arranged by size:
3476 2573 1160 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8365
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2369 2565 4773
Size: 416 53 83 399 24 952 196 2208
Cuts at: 4773 5092 6096 7054 442
Size: 319 1004 958 1753
Fragments arranged by size:
2208 1753 1004 958 952 416 399 319
196 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8365
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4067 4067
Size: 8365
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2283 2349 3696 4569 4798 4872 4983 5115
Size: 1044 66 1347 873 229 74 111 132
Cuts at: 5115 7025 1239
Size: 1910 2579
Fragments arranged by size:
2579 1910 1347 1044 873 229 132 111
74 66
Bpu10I CC'TnA_GC
Cuts at: 3320 4281 4689 3320
Size: 961 408 6996
Fragments arranged by size:
6996 961 408
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2153 4047 4113 4191 6490 7355 7653 2153
Size: 1894 66 78 2299 865 298 2865
Fragments arranged by size:
2865 2299 1894 865 298 78 66
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5597 7028 882
Size: 617 4098 1431 2219
Fragments arranged by size:
4098 2219 1431 617
BsaAI yAC'GTr
Cuts at: 493 1204 6156 493
Size: 711 4952 2702
Fragments arranged by size:
4952 2702 711
BsaBI GATnn'nnATC
Cuts at: 8359 8359
Size: 8365
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6172 6554 7189 275
Size: 53 83 186 936 4639 382 635 1451
Fragments arranged by size:
4639 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2712 6798 7196 7470 7617 8028 2712
Size: 4086 398 274 147 411 3049
Fragments arranged by size:
4086 3049 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3534 3564 4368 4398 4413
Size: 30 165 30 2131 30 804 30 15
Cuts at: 4413 4443 5447 5477 1178
Size: 30 1004 30 4066
Fragments arranged by size:
4066 2131 1004 804 165 30 30 30
30 30 30 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2185 3608 4695 4827 1186
Size: 999 1423 1087 132 4724
Fragments arranged by size:
4724 1423 1087 999 132
BseYI C'CCAG_C
Cuts at: 4644 5656 7568 8064 4644
Size: 1012 1912 496 4945
Fragments arranged by size:
4945 1912 1012 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3371 4107 5321 1163
Size: 573 1635 736 1214 4207
Fragments arranged by size:
4207 1635 1214 736 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5815 6576 7604 1540
Size: 275 186 3814 761 1028 2301
Fragments arranged by size:
3814 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3633 4372 4885 5352 6426 6511 7582 3633
Size: 739 513 467 1074 85 1071 4416
Fragments arranged by size:
4416 1074 1071 739 513 467 85
BsiWI C'GTAC_G
Cuts at: 4413 4413
Size: 8365
BsmI GAATG_Cn'
Cuts at: 2128 2649 3243 5876 5969 2128
Size: 521 594 2633 93 4524
Fragments arranged by size:
4524 2633 594 521 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 3602 3633 4372 4885 5266 5275
Size: 399 261 1551 31 739 513 381 9
Cuts at: 5275 5352 5684 6426 6511 7582 1391
Size: 77 332 742 85 1071 2174
Fragments arranged by size:
2174 1551 1071 742 739 513 399 381
332 261 85 77 31 9
BspEI T'CCGG_A
Cuts at: 8028 8028
Size: 8365
BspHI T'CATG_A
Cuts at: 5076 6254 7984 5076
Size: 1178 1730 5457
Fragments arranged by size:
5457 1730 1178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2267 3806 5720 844
Size: 1423 1539 1914 3489
Fragments arranged by size:
3489 1914 1539 1423
BsrBI CCG'CTC
Cuts at: 1394 1823 6252 1394
Size: 429 4429 3507
Fragments arranged by size:
4429 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2523 4683 5520 6860 7034 65
Size: 1183 1275 2160 837 1340 174 1396
Fragments arranged by size:
2160 1396 1340 1275 1183 837 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2712 7009 7196 1700
Size: 110 902 4297 187 2869
Fragments arranged by size:
4297 2869 902 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8365
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8365
BssSI C'ACGA_G
Cuts at: 3459 6425 7437 3459
Size: 2966 1012 4387
Fragments arranged by size:
4387 2966 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3349 5308 794
Size: 2555 1959 3851
Fragments arranged by size:
3851 2555 1959
BstBI TT'CG_AA
Cuts at: 3175 7169 3175
Size: 3994 4371
BstEII G'GTnAC_C
Cuts at: 1184 2715 5090 1184
Size: 1531 2375 4459
Fragments arranged by size:
4459 2375 1531
BstXI CCAn_nnnn'nTGG
Cuts at: 3805 4273 5656 3805
Size: 468 1383 6514
Fragments arranged by size:
6514 1383 468
BstYI r'GATC_y
Cuts at: 1972 2455 2593 2737 2818 3499 4159 5377 5695
Size: 483 138 144 81 681 660 1218 318
Cuts at: 5695 6062 6447 6464 7916 8002 8014 8360 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1218 681 660 483 385 367
346 318 144 138 86 81 17 12
BtgI C'CryG_G
Cuts at: 513 1065 4212 4504 5194 513
Size: 552 3147 292 690 3684
Fragments arranged by size:
3684 3147 690 552 292
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7626
Fragments arranged by size:
7626 683 56
BtsI GCAGTG_nn'
Cuts at: 3891 3903 4907 4931 5881 6675 6695 3891
Size: 12 1004 24 950 794 20 5561
Fragments arranged by size:
5561 1004 950 794 24 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8365
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8330
DraI TTT'AAA
Cuts at: 5669 5669
Size: 8365
DraIII CAC_nnn'GTG
Cuts at: 2765 2916 3283 3584 2765
Size: 151 367 301 7546
Fragments arranged by size:
7546 367 301 151
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7372 809
Size: 507 6056 1802
Fragments arranged by size:
6056 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2575 3789 3807
Size: 54 1425 50 275 18 745 1214 18
Cuts at: 3807 4459 5182 5812 6701 8330 8
Size: 652 723 630 889 1629 43
Fragments arranged by size:
1629 1425 1214 889 745 723 652 630
275 54 50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 5812 1537
Size: 275 4000 4090
Fragments arranged by size:
4090 4000 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2203 3606 4807 6295 1353
Size: 850 1403 1201 1488 3423
Fragments arranged by size:
3423 1488 1403 1201 850
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4887 6952 7326 7472 387
Size: 1064 138 3298 2065 374 146 1280
Fragments arranged by size:
3298 2065 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4883 4883
Size: 8365
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2283 2349 3173 3323 3630 3696 4139
Size: 393 651 66 824 150 307 66 443
Cuts at: 4139 4569 4798 4872 4881 4983 5109 5115 5385
Size: 430 229 74 9 102 126 6 270
Cuts at: 5385 5581 6428 7025 7812 8080 1239
Size: 196 847 597 787 268 1524
Fragments arranged by size:
1524 847 824 787 651 597 443 430
393 307 270 268 229 196 150 126
102 74 66 66 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 4386 4386
Size: 8365
EcoO109I rG'GnC_Cy
Cuts at: 2228 4973 2228
Size: 2745 5620
EcoRI G'AATT_C
Cuts at: 5674 6052 5674
Size: 378 7987
EcoRV GAT'ATC
Cuts at: 2515 2515
Size: 8365
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3460 4161 5169 3460
Size: 701 1008 6656
Fragments arranged by size:
6656 1008 701
FspI TGC'GCA
Cuts at: 6871 6871
Size: 8365
HaeII r_GCGC'y
Cuts at: 1678 5392 7512 1678
Size: 3714 2120 2531
Fragments arranged by size:
3714 2531 2120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2162 2194 2722 2754 3813
Size: 32 135 32 590 32 528 32 1059
Cuts at: 3813 3845 4111 4143 5084 5116 7006 7038 7080
Size: 32 266 32 941 32 1890 32 42
Cuts at: 7080 7112 1373
Size: 32 2626
Fragments arranged by size:
2626 1890 1059 941 590 528 266 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3043 3763 5709 5955 669
Size: 988 281 1105 720 1946 246 3079
Fragments arranged by size:
3079 1946 1105 988 720 281 246
HindIII A'AGCT_T
Cuts at: 748 3047 748
Size: 2299 6066
HpaI GTT'AAC
Cuts at: 5955 5955
Size: 8365
KpnI G_GTAC'C
Cuts at: 2212 4610 5690 2212
Size: 2398 1080 4887
Fragments arranged by size:
4887 2398 1080
MfeI C'AATT_G
Cuts at: 2099 2799 5964 2099
Size: 700 3165 4500
Fragments arranged by size:
4500 3165 700
MluI A'CGCG_T
Cuts at: 8241 8241
Size: 8365
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4097 4967 7479 7663 1305
Size: 2792 870 2512 184 2007
Fragments arranged by size:
2792 2512 2007 870 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3809 4461 5184 10
Size: 54 1425 343 1977 652 723 3191
Fragments arranged by size:
3191 1977 1425 723 652 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2673 2917 3896 4545 6323 6682 6841
Size: 648 1507 244 979 649 1778 359 159
Cuts at: 6841 518
Size: 2042
Fragments arranged by size:
2042 1778 1507 979 649 648 359 244
159
MspA1I CmG'CkG
Cuts at: 1606 2520 2966 4777 5781 6458 7606 7851 1606
Size: 914 446 1811 1004 677 1148 245 2120
Fragments arranged by size:
2120 1811 1148 1004 914 677 446 245
NcoI C'CATG_G
Cuts at: 513 1065 4212 4504 5194 513
Size: 552 3147 292 690 3684
Fragments arranged by size:
3684 3147 690 552 292
NdeI CA'TA_TG
Cuts at: 387 2450 4593 387
Size: 2063 2143 4159
Fragments arranged by size:
4159 2143 2063
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8365
NotI GC'GGCC_GC
Cuts at: 5812 5812
Size: 8365
NsiI A_TGCA'T
Cuts at: 2649 4542 5571 2649
Size: 1893 1029 5443
Fragments arranged by size:
5443 1893 1029
NspI r_CATG'y
Cuts at: 1132 1229 4071 4116 5230 5300 5723 7268 1132
Size: 97 2842 45 1114 70 423 1545 2229
Fragments arranged by size:
2842 2229 1545 1114 423 97 70 45
PasI CC'CwG_GG
Cuts at: 1348 5267 1348
Size: 3919 4446
PciI A'CATG_T
Cuts at: 7264 7264
Size: 8365
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2589 4457 1108
Size: 1481 1868 5016
Fragments arranged by size:
5016 1868 1481
PfoI T'CCnGG_A
Cuts at: 2769 4915 2769
Size: 2146 6219
PmeI GTTT'AAAC
Cuts at: 5669 5669
Size: 8365
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2077 2109 4960 4992 6434 6466 7947 7979 2077
Size: 32 2851 32 1442 32 1481 32 2463
Fragments arranged by size:
2851 2463 1481 1442 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4973 4973
Size: 8365
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8365
PsiI TTA'TAA
Cuts at: 2235 5935 2235
Size: 3700 4665
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4912 4944 4912
Size: 32 8333
PstI C_TGCA'G
Cuts at: 830 3898 3970 4059 4300 5115 5717 830
Size: 3068 72 89 241 815 602 3478
Fragments arranged by size:
3478 3068 815 602 241 89 72
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8365
PvuII CAG'CTG
Cuts at: 2520 2966 4777 2520
Size: 446 1811 6108
Fragments arranged by size:
6108 1811 446
SacI G_AGCT'C
Cuts at: 4885 4885
Size: 8365
SalI G'TCGA_C
Cuts at: 1936 5707 1936
Size: 3771 4594
SapI GCTCTTCn'nnn_
Cuts at: 3606 3606
Size: 8365
SbfI CC_TGCA'GG
Cuts at: 5717 5717
Size: 8365
ScaI AGT'ACT
Cuts at: 4225 6613 8325 4225
Size: 2388 1712 4265
Fragments arranged by size:
4265 2388 1712
SfcI C'TryA_G
Cuts at: 826 1045 3894 3966 4055 4227 4296 4383 5111
Size: 219 2849 72 89 172 69 87 728
Cuts at: 5111 5713 6848 7529 7720 826
Size: 602 1135 681 191 1471
Fragments arranged by size:
2849 1471 1135 728 681 602 219 191
172 89 87 72 69
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8365
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8365
SmaI CCC'GGG
Cuts at: 1872 5692 1872
Size: 3820 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2132 4062 4128 4206 6469 7370
Size: 197 926 189 1930 66 78 2263 901
Cuts at: 7370 7632 820
Size: 262 1553
Fragments arranged by size:
2263 1930 1553 926 901 262 197 189
78 66
SnaBI TAC'GTA
Cuts at: 493 6156 493
Size: 5663 2702
SpeI A'CTAG_T
Cuts at: 152 4220 152
Size: 4068 4297
SphI G_CATG'C
Cuts at: 1132 5230 5723 1132
Size: 4098 493 3774
Fragments arranged by size:
4098 3774 493
SspI AAT'ATT
Cuts at: 5 52 2296 4496 5254 6289 5
Size: 47 2244 2200 758 1035 2081
Fragments arranged by size:
2244 2200 2081 1035 758 47
StuI AGG'CCT
Cuts at: 1517 3181 4444 4799 1517
Size: 1664 1263 355 5083
Fragments arranged by size:
5083 1664 1263 355
StyI C'CwwG_G
Cuts at: 513 1065 2166 2385 4212 4302 4434 4504 5194
Size: 552 1101 219 1827 90 132 70 690
Cuts at: 5194 5804 513
Size: 610 3074
Fragments arranged by size:
3074 1827 1101 690 610 552 219 132
90 70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4616 5078 5648 5657 6392 6409 6562
Size: 1255 3036 462 570 9 735 17 153
Cuts at: 6562 6747 7192 325
Size: 185 445 1498
Fragments arranged by size:
3036 1498 1255 735 570 462 445 185
153 17 9
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4223 6611 8323
Size: 274 80 33 51 1443 2246 2388 1712
Cuts at: 8323 96
Size: 138
Fragments arranged by size:
2388 2246 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2751 2783 3534 3566 8325 8357 2751
Size: 32 751 32 4759 32 2759
Fragments arranged by size:
4759 2759 751 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8365
XbaI T'CTAG_A
Cuts at: 5701 5701
Size: 8365
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2791 5192 5449 2791
Size: 2401 257 5707
Fragments arranged by size:
5707 2401 257
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8365
XmaI C'CCGG_G
Cuts at: 1870 5690 1870
Size: 3820 4545
XmnI GAAnn'nnTTC
Cuts at: 2399 3241 6494 2399
Size: 842 3253 4270
Fragments arranged by size:
4270 3253 842
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6173 276
Size: 53 83 186 936 4639 2468
Fragments arranged by size:
4639 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BbeI BsmBI BstZ17I Bsu36I
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PspXI RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI