Restriction map for
pFN21AA0904
(Circular) (Six-base) MAPSORT of: pFN21AA0904.seq Check: 7837 from: 1 to: 9148
pFN21AA0904 9148 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5931 5931
Size: 9148
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5152 6958 278
Size: 53 83 186 936 3616 1806 2468
Fragments arranged by size:
3616 2468 1806 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4948 6491 1937
Size: 3011 1543 4594
Fragments arranged by size:
4594 3011 1543
Acc65I G'GTAC_C
Cuts at: 4704 6469 4704
Size: 1765 7383
AclI AA'CG_TT
Cuts at: 1563 3881 4738 7275 7648 1563
Size: 2318 857 2537 373 3063
Fragments arranged by size:
3063 2537 2318 857 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2594 3414 4289 4683 5559 5922 6260 7211
Size: 962 820 875 394 876 363 338 951
Cuts at: 7211 8595 8863 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 962 951 876 875 820 394
363 338 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8951
AflIII A'CryG_T
Cuts at: 1203 3989 8047 9024 1203
Size: 2786 4058 977 1327
Fragments arranged by size:
4058 2786 1327 977
AgeI A'CCGG_T
Cuts at: 7979 7979
Size: 9148
AhdI GACnn_n'nnGTC
Cuts at: 7877 7877
Size: 9148
AleI CACnn'nnGTG
Cuts at: 1166 4356 1166
Size: 3190 5958
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9148
AlwNI CAG_nnn'CTG
Cuts at: 3230 3383 5452 6085 6891 8463 3230
Size: 153 2069 633 806 1572 3915
Fragments arranged by size:
3915 2069 1572 806 633 153
ApaI G_GGCC'C
Cuts at: 5727 6362 5727
Size: 635 8513
ApaLI G'TGCA_C
Cuts at: 4490 5029 7205 8361 4490
Size: 539 2176 1156 5277
Fragments arranged by size:
5277 2176 1156 539
ApoI r'AATT_y
Cuts at: 1493 3424 3802 4113 4564 4958 6457 6683 6835
Size: 1931 378 311 451 394 1499 226 152
Cuts at: 6835 8944 1493
Size: 2109 1697
Fragments arranged by size:
2109 1931 1697 1499 451 394 378 311
226 152
AseI AT'TA_AT
Cuts at: 160 1032 7702 160
Size: 872 6670 1606
Fragments arranged by size:
6670 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9148
AvaI C'yCGr_G
Cuts at: 1870 1943 2361 2716 2770 2812 6473 1870
Size: 73 418 355 54 42 3661 4545
Fragments arranged by size:
4545 3661 418 355 73 54 42
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3825 3858 3825
Size: 33 9115
BamHI G'GATC_C
Cuts at: 6478 6845 6478
Size: 367 8781
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4424 4704 6469 7924 618
Size: 325 217 627 2637 280 1765 1455 1842
Fragments arranged by size:
2637 1842 1765 1455 627 325 280 217
BanII G_rGCy'C
Cuts at: 1391 2672 2885 4029 5727 5784 6362 6467 1391
Size: 1281 213 1144 1698 57 578 105 4072
Fragments arranged by size:
4072 1698 1281 1144 578 213 105 57
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2558 3839 4147 6363 928
Size: 971 659 1281 308 2216 3713
Fragments arranged by size:
3713 2216 1281 971 659 308
BbvCI CC'TCA_GC
Cuts at: 4086 5265 6246 4086
Size: 1179 981 6988
Fragments arranged by size:
6988 1179 981
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2318 2352 7339 7373 1903
Size: 34 50 34 297 34 4987 34 3678
Fragments arranged by size:
4987 3678 297 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7040 8250 7040
Size: 1210 7938
BclI T'GATC_A
Cuts at: 1548 4845 1548
Size: 3297 5851
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5722 7759 136
Size: 107 122 71 631 605 4050 2037 1525
Fragments arranged by size:
4050 2037 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2903 4039 9143 2903
Size: 1136 5104 2908
Fragments arranged by size:
5104 2908 1136
BlpI GC'TnA_GC
Cuts at: 4833 6565 4833
Size: 1732 7416
Bme1580I G_kGCm'C
Cuts at: 1790 2198 2586 4494 5033 5482 5727 6152 6362
Size: 408 388 1908 539 449 245 425 210
Cuts at: 6362 7209 8365 1790
Size: 847 1156 2573
Fragments arranged by size:
2573 1908 1156 847 539 449 425 408
388 245 210
BmgBI CAC'GTC
Cuts at: 1318 3992 1318
Size: 2674 6474
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2970 3042 4168
Size: 416 53 83 399 24 1553 72 1126
Cuts at: 4168 5555 6330 6361 6879 7837 442
Size: 1387 775 31 518 958 1753
Fragments arranged by size:
1753 1553 1387 1126 958 775 518 416
399 83 72 53 31 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9148
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3215 5415 3215
Size: 2200 6948
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2398 3936 4005 4044 4698 5079 5705 7808
Size: 1159 1538 69 39 654 381 626 2103
Cuts at: 7808 1239
Size: 2579
Fragments arranged by size:
2579 2103 1538 1159 654 626 381 69
39
Bpu10I CC'TnA_GC
Cuts at: 3014 3315 4086 5265 5390 5778 6205 6246 6334
Size: 301 771 1179 125 388 427 41 88
Cuts at: 6334 3014
Size: 5828
Fragments arranged by size:
5828 1179 771 427 388 301 125 88
41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3822 5665 5965 7273 8138 8436 3822
Size: 1843 300 1308 865 298 4534
Fragments arranged by size:
4534 1843 1308 865 300 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3030 3569 4099 5510 5941 6272 7811
Size: 617 1531 539 530 1411 431 331 1539
Cuts at: 7811 882
Size: 2219
Fragments arranged by size:
2219 1539 1531 1411 617 539 530 431
331
BsaAI yAC'GTr
Cuts at: 493 1204 6939 493
Size: 711 5735 2702
Fragments arranged by size:
5735 2702 711
BsaBI GATnn'nnATC
Cuts at: 9142 9142
Size: 9148
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4945 5149 6955 7337
Size: 53 83 186 936 3412 204 1806 382
Cuts at: 7337 7972 275
Size: 635 1451
Fragments arranged by size:
3412 1806 1451 936 635 382 204 186
83 53
BsaWI w'CCGG_w
Cuts at: 2439 2592 7581 7979 8253 8400 8811 2439
Size: 153 4989 398 274 147 411 2776
Fragments arranged by size:
4989 2776 411 398 274 153 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2237 2267 2561 2587 2591
Size: 30 165 30 834 30 294 26 4
Cuts at: 2591 2617 3619 3649 3871 3901 4245 4275 5411
Size: 26 1002 30 222 30 344 30 1136
Cuts at: 5411 5441 1178
Size: 30 4885
Fragments arranged by size:
4885 1136 1002 834 344 294 222 165
30 30 30 30 30 30 30 26
26 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2064 2486 3548 5082 5546 5768 6450 1186
Size: 878 422 1062 1534 464 222 682 3884
Fragments arranged by size:
3884 1534 1062 878 682 464 422 222
BseYI C'CCAG_C
Cuts at: 5254 5297 5499 5860 5895 6304 8351 8847 5254
Size: 43 202 361 35 409 2047 496 5555
Fragments arranged by size:
5555 2047 496 409 361 202 43 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2312 6182 1163
Size: 573 576 3870 4129
Fragments arranged by size:
4129 3870 576 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2323 2933 6598 7359 8387 1540
Size: 275 186 322 610 3665 761 1028 2301
Fragments arranged by size:
3665 2301 1028 761 610 322 275 186
BsiHKAI G_wGCw'C
Cuts at: 4029 4494 5033 5937 7209 7294 8365 4029
Size: 465 539 904 1272 85 1071 4812
Fragments arranged by size:
4812 1272 1071 904 539 465 85
BsmI GAATG_Cn'
Cuts at: 6659 6752 6659
Size: 93 9055
BsmBI CGTCTCn'nnnn_
Cuts at: 2920 2920
Size: 9148
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2198 2586 2672 2885 4029 4494 5033
Size: 399 408 388 86 213 1144 465 539
Cuts at: 5033 5482 5727 5784 5937 6152 6362 6467 7209
Size: 449 245 57 153 215 210 105 742
Cuts at: 7209 7294 8365 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1144 1071 742 539 465 449 408
399 388 245 215 213 210 153 105
86 85 57
BspEI T'CCGG_A
Cuts at: 8811 8811
Size: 9148
BspHI T'CATG_A
Cuts at: 4521 7037 8767 4521
Size: 2516 1730 4902
Fragments arranged by size:
4902 2516 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2488 5376 5931 6503 844
Size: 1644 2888 555 572 3489
Fragments arranged by size:
3489 2888 1644 572 555
BsrBI CCG'CTC
Cuts at: 1394 1823 7035 1394
Size: 429 5212 3507
Fragments arranged by size:
5212 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 7643 7817 65
Size: 1183 6395 174 1396
Fragments arranged by size:
6395 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7792 7979 1700
Size: 110 5982 187 2869
Fragments arranged by size:
5982 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3817 96
Size: 3721 5427
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9148
BssSI C'ACGA_G
Cuts at: 7208 8220 7208
Size: 1012 8136
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3230 5555 794
Size: 2436 2325 4387
Fragments arranged by size:
4387 2436 2325
BstBI TT'CG_AA
Cuts at: 2465 7952 2465
Size: 5487 3661
BstEII G'GTnAC_C
Cuts at: 1184 2170 2874 1184
Size: 986 704 7458
Fragments arranged by size:
7458 986 704
BstXI CCAn_nnnn'nTGG
Cuts at: 2161 4881 2161
Size: 2720 6428
BstYI r'GATC_y
Cuts at: 1972 2660 2903 3125 4039 5014 6478 6845 7230
Size: 688 243 222 914 975 1464 367 385
Cuts at: 7230 7247 8699 8785 8797 9143 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1464 1452 975 914 688 385 367
346 243 222 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5218 5218
Size: 9148
BtgI C'CryG_G
Cuts at: 513 1065 5520 513
Size: 552 4455 4141
Fragments arranged by size:
4455 4141 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8409
Fragments arranged by size:
8409 683 56
BtsI GCAGTG_nn'
Cuts at: 5361 6664 7458 7478 5361
Size: 1303 794 20 7031
Fragments arranged by size:
7031 1303 794 20
ClaI AT'CG_AT
Cuts at: 1554 2429 1554
Size: 875 8273
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5692 5727 529
Size: 35 5128 35 3950
Fragments arranged by size:
5128 3950 35 35
DraI TTT'AAA
Cuts at: 6452 6452
Size: 9148
DraIII CAC_nnn'GTG
Cuts at: 6220 6220
Size: 9148
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8155 809
Size: 507 6839 1802
Fragments arranged by size:
6839 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2731 4219 5917
Size: 54 1425 50 275 18 901 1488 1698
Cuts at: 5917 6595 7484 9113 8
Size: 678 889 1629 43
Fragments arranged by size:
1698 1629 1488 1425 901 889 678 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6595 1537
Size: 275 4783 4090
Fragments arranged by size:
4783 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3116 3594 3687 3954 4659 5172 5762 6421
Size: 1763 478 93 267 705 513 590 659
Cuts at: 6421 7078 1353
Size: 657 3423
Fragments arranged by size:
3423 1763 705 659 657 590 513 478
267 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7735 8109 8255 387
Size: 1064 138 6146 374 146 1280
Fragments arranged by size:
6146 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4027 4027
Size: 9148
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2398 2594 3414 3936 4005 4044 4289
Size: 393 766 196 820 522 69 39 245
Cuts at: 4289 4683 4698 5079 5559 5705 5922 6260 7211
Size: 394 15 381 480 146 217 338 951
Cuts at: 7211 7808 8595 8863 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 951 820 787 766 597 522 480
394 393 381 338 268 245 217 196
146 69 39 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2815 2890 2920 6012 2815
Size: 75 30 3092 5951
Fragments arranged by size:
5951 3092 75 30
EcoO109I rG'GnC_Cy
Cuts at: 5723 5724 5772 6099 6181 6199 6358 6398 5723
Size: 1 48 327 82 18 159 40 8473
Fragments arranged by size:
8473 327 159 82 48 40 18 1
EcoRI G'AATT_C
Cuts at: 4958 6457 6835 4958
Size: 1499 378 7271
Fragments arranged by size:
7271 1499 378
EcoRV GAT'ATC
Cuts at: 2230 2445 2230
Size: 215 8933
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2973 3965 5849 2973
Size: 992 1884 6272
Fragments arranged by size:
6272 1884 992
FspI TGC'GCA
Cuts at: 7654 7654
Size: 9148
HaeII r_GCGC'y
Cuts at: 1678 5844 8295 1678
Size: 4166 2451 2531
Fragments arranged by size:
4166 2531 2451
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2219 2237 2251 2269 2429
Size: 32 135 32 647 18 14 18 160
Cuts at: 2429 2461 2561 2587 2593 2619 2699 2731 3022
Size: 32 100 26 6 26 80 32 291
Cuts at: 3022 3054 3446 3478 3529 3561 3976 4008 4999
Size: 32 392 32 51 32 415 32 991
Cuts at: 4999 5031 7789 7821 7863 7895 1373
Size: 32 2758 32 42 32 2626
Fragments arranged by size:
2758 2626 991 647 415 392 291 160
135 100 80 51 42 32 32 32
32 32 32 32 32 32 32 32
26 26 18 18 14 6
HincII GTy'rAC
Cuts at: 669 1657 1938 4366 6492 6738 669
Size: 988 281 2428 2126 246 3079
Fragments arranged by size:
3079 2428 2126 988 281 246
HindIII A'AGCT_T
Cuts at: 748 5598 748
Size: 4850 4298
HpaI GTT'AAC
Cuts at: 4366 6738 4366
Size: 2372 6776
KpnI G_GTAC'C
Cuts at: 4708 6473 4708
Size: 1765 7383
MfeI C'AATT_G
Cuts at: 3597 6747 3597
Size: 3150 5998
MluI A'CGCG_T
Cuts at: 9024 9024
Size: 9148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2268 3014 3432 5454 8262 8446 1305
Size: 963 746 418 2022 2808 184 2007
Fragments arranged by size:
2808 2022 2007 963 746 418 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2733 4221 5919 10
Size: 54 1425 343 901 1488 1698 3239
Fragments arranged by size:
3239 1698 1488 1425 901 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4356 7106 7465 7624 518
Size: 648 3190 2750 359 159 2042
Fragments arranged by size:
3190 2750 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2964 3227 3854 4877 5036 5335 5413 5452
Size: 1358 263 627 1023 159 299 78 39
Cuts at: 5452 5615 5653 6564 7241 8389 8634 1606
Size: 163 38 911 677 1148 245 2120
Fragments arranged by size:
2120 1358 1148 1023 911 677 627 299
263 245 163 159 78 39 38
NcoI C'CATG_G
Cuts at: 513 1065 5520 513
Size: 552 4455 4141
Fragments arranged by size:
4455 4141 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9148
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9148
NotI GC'GGCC_GC
Cuts at: 6595 6595
Size: 9148
NspI r_CATG'y
Cuts at: 1132 1229 5005 5623 6275 6506 8051 1132
Size: 97 3776 618 652 231 1545 2229
Fragments arranged by size:
3776 2229 1545 652 618 231 97
PasI CC'CwG_GG
Cuts at: 1348 2191 1348
Size: 843 8305
PciI A'CATG_T
Cuts at: 8047 8047
Size: 9148
PflMI CCAn_nnn'nTGG
Cuts at: 1108 6085 1108
Size: 4977 4171
PfoI T'CCnGG_A
Cuts at: 2663 2800 2663
Size: 137 9011
PmeI GTTT'AAAC
Cuts at: 6452 6452
Size: 9148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3115 3147 7217 7249 8730 8762 3115
Size: 32 4070 32 1481 32 3501
Fragments arranged by size:
4070 3501 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 6099 6181 6199 6099
Size: 82 18 9048
Fragments arranged by size:
9048 82 18
PshAI GACnn'nnGTC
Cuts at: 1613 2298 2748 2932 1613
Size: 685 450 184 7829
Fragments arranged by size:
7829 685 450 184
PsiI TTA'TAA
Cuts at: 6718 6718
Size: 9148
PspOMI G'GGCC_C
Cuts at: 5723 6358 5723
Size: 635 8513
PspXI vC'TCGA_Gb
Cuts at: 2770 2770
Size: 9148
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5031 5063 5031
Size: 32 9116
PstI C_TGCA'G
Cuts at: 830 3241 4981 5376 6500 830
Size: 2411 1740 395 1124 3478
Fragments arranged by size:
3478 2411 1740 1124 395
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9148
PvuII CAG'CTG
Cuts at: 3227 3854 4877 5036 5335 5413 5452 5615 5653
Size: 627 1023 159 299 78 39 163 38
Cuts at: 5653 3227
Size: 6722
Fragments arranged by size:
6722 1023 627 299 163 159 78 39
38
SacI G_AGCT'C
Cuts at: 4029 4029
Size: 9148
SalI G'TCGA_C
Cuts at: 1936 6490 1936
Size: 4554 4594
SanDI GG'GwC_CC
Cuts at: 6199 6199
Size: 9148
SapI GCTCTTCn'nnn_
Cuts at: 3687 5172 3687
Size: 1485 7663
SbfI CC_TGCA'GG
Cuts at: 6500 6500
Size: 9148
ScaI AGT'ACT
Cuts at: 3745 5230 5960 7396 9108 3745
Size: 1485 730 1436 1712 3785
Fragments arranged by size:
3785 1712 1485 1436 730
SfcI C'TryA_G
Cuts at: 826 1045 2887 2917 3237 4977 5048 5372 6496
Size: 219 1842 30 320 1740 71 324 1124
Cuts at: 6496 7631 8312 8503 826
Size: 1135 681 191 1471
Fragments arranged by size:
1842 1740 1471 1135 1124 681 324 320
219 191 71 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 5722 5722
Size: 9148
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9148
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9148
SmaI CCC'GGG
Cuts at: 1872 2363 6475 1872
Size: 491 4112 4545
Fragments arranged by size:
4545 4112 491
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2770 2812 3837 5644 5944 7252
Size: 197 926 827 42 1025 1807 300 1308
Cuts at: 7252 8153 8415 820
Size: 901 262 1553
Fragments arranged by size:
1807 1553 1308 1025 926 901 827 300
262 197 42
SnaBI TAC'GTA
Cuts at: 493 6939 493
Size: 6446 2702
SpeI A'CTAG_T
Cuts at: 152 4258 152
Size: 4106 5042
SphI G_CATG'C
Cuts at: 1132 6506 1132
Size: 5374 3774
SspI AAT'ATT
Cuts at: 5 52 7072 5
Size: 47 7020 2081
Fragments arranged by size:
7020 2081 47
StuI AGG'CCT
Cuts at: 1517 5313 5942 6345 1517
Size: 3796 629 403 4320
Fragments arranged by size:
4320 3796 629 403
StyI C'CwwG_G
Cuts at: 513 1065 3264 4236 5520 6072 6124 6587 513
Size: 552 2199 972 1284 552 52 463 3074
Fragments arranged by size:
3074 2199 1284 972 552 552 463 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2295 2402 2638 2815 2989 7175 7192
Size: 1255 715 107 236 177 174 4186 17
Cuts at: 7192 7345 7530 7975 325
Size: 153 185 445 1498
Fragments arranged by size:
4186 1498 1255 715 445 236 185 177
174 153 107 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3743 3817 5228
Size: 274 80 33 51 1443 1766 74 1411
Cuts at: 5228 5958 7394 9106 96
Size: 730 1436 1712 138
Fragments arranged by size:
1766 1712 1443 1436 1411 730 274 138
80 74 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2342 2374 3994 4026 4156 4188 4245 4277 4571
Size: 32 1620 32 130 32 57 32 294
Cuts at: 4571 4603 9108 9140 2342
Size: 32 4505 32 2350
Fragments arranged by size:
4505 2350 1620 294 130 57 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5973 1316
Size: 4657 4491
XbaI T'CTAG_A
Cuts at: 6484 6484
Size: 9148
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4825 4825
Size: 9148
XhoI C'TCGA_G
Cuts at: 1943 2770 2812 1943
Size: 827 42 8279
Fragments arranged by size:
8279 827 42
XmaI C'CCGG_G
Cuts at: 1870 2361 6473 1870
Size: 491 4112 4545
Fragments arranged by size:
4545 4112 491
XmnI GAAnn'nnTTC
Cuts at: 3948 7277 3948
Size: 3329 5819
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5150 6956 276
Size: 53 83 186 936 3616 1806 2468
Fragments arranged by size:
3616 2468 1806 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BfrBI BsiWI BstZ17I
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI RsrII SacII SexAI SfoI SrfI SwaI