Restriction map for
pFN21AA0879
(Circular) (Six-base) MAPSORT of: pFN21AA0879.seq Check: 8629 from: 1 to: 6064
pFN21AA0879 6064 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3874 278
Size: 53 83 186 936 2338 2468
Fragments arranged by size:
2468 2338 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3407 1937
Size: 1470 4594
Acc65I G'GTAC_C
Cuts at: 3385 3385
Size: 6064
AclI AA'CG_TT
Cuts at: 1563 4191 4564 1563
Size: 2628 373 3063
Fragments arranged by size:
3063 2628 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2095 2147 2363 2616 3311 4127 5511 5779
Size: 463 52 216 253 695 816 1384 268
Cuts at: 5779 1632
Size: 1917
Fragments arranged by size:
1917 1384 816 695 463 268 253 216
52
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5867
AflIII A'CryG_T
Cuts at: 1203 4963 5940 1203
Size: 3760 977 1327
Fragments arranged by size:
3760 1327 977
AgeI A'CCGG_T
Cuts at: 4895 4895
Size: 6064
AhdI GACnn_n'nnGTC
Cuts at: 3171 4793 3171
Size: 1622 4442
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6064
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6064
AlwNI CAG_nnn'CTG
Cuts at: 2810 3807 5379 2810
Size: 997 1572 3495
Fragments arranged by size:
3495 1572 997
ApaLI G'TGCA_C
Cuts at: 4121 5277 4121
Size: 1156 4908
ApoI r'AATT_y
Cuts at: 1493 2138 2155 2934 3373 3599 3751 5860 1493
Size: 645 17 779 439 226 152 2109 1697
Fragments arranged by size:
2109 1697 779 645 439 226 152 17
AseI AT'TA_AT
Cuts at: 160 1032 3210 4618 160
Size: 872 2178 1408 1606
Fragments arranged by size:
2178 1606 1408 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
AvaI C'yCGr_G
Cuts at: 1870 1943 3389 1870
Size: 73 1446 4545
Fragments arranged by size:
4545 1446 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2409 2442 2409
Size: 33 6031
BamHI G'GATC_C
Cuts at: 2329 3394 3761 2329
Size: 1065 367 4632
Fragments arranged by size:
4632 1065 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3385 4840 618
Size: 325 217 627 1598 1455 1842
Fragments arranged by size:
1842 1598 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2804 3383 1391
Size: 1413 579 4072
Fragments arranged by size:
4072 1413 579
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2901 928
Size: 971 1002 4091
Fragments arranged by size:
4091 1002 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4255 4289 1903
Size: 34 2318 34 3678
Fragments arranged by size:
3678 2318 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2107 3956 5166 2107
Size: 1849 1210 3005
Fragments arranged by size:
3005 1849 1210
BclI T'GATC_A
Cuts at: 1548 2700 2713 1548
Size: 1152 13 4899
Fragments arranged by size:
4899 1152 13
BfrBI ATG'CAT
Cuts at: 3040 3040
Size: 6064
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4675 136
Size: 107 122 71 631 605 3003 1525
Fragments arranged by size:
3003 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6059 6059
Size: 6064
BlpI GC'TnA_GC
Cuts at: 3481 3481
Size: 6064
Bme1580I G_kGCm'C
Cuts at: 1790 4125 5281 1790
Size: 2335 1156 2573
Fragments arranged by size:
2573 2335 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6064
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2722 2798 3795
Size: 416 53 83 399 24 1305 76 997
Cuts at: 3795 4753 442
Size: 958 1753
Fragments arranged by size:
1753 1305 997 958 416 399 83 76
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6064
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2135 4724 1239
Size: 896 2589 2579
Fragments arranged by size:
2589 2579 896
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2651 4189 5054 5352 2651
Size: 1538 865 298 3363
Fragments arranged by size:
3363 1538 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4727 882
Size: 617 3228 2219
Fragments arranged by size:
3228 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3855 493
Size: 711 2651 2702
Fragments arranged by size:
2702 2651 711
BsaBI GATnn'nnATC
Cuts at: 3114 3236 3348 6058 3114
Size: 122 112 2710 3120
Fragments arranged by size:
3120 2710 122 112
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3871 4253 4888 275
Size: 53 83 186 936 2338 382 635 1451
Fragments arranged by size:
2338 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4497 4895 5169 5316 5727 4497
Size: 398 274 147 411 4834
Fragments arranged by size:
4834 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5839
Fragments arranged by size:
5839 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2461 1186
Size: 1275 4789
BseYI C'CCAG_C
Cuts at: 5267 5763 5267
Size: 496 5568
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5491
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3514 4275 5303 1540
Size: 275 186 1513 761 1028 2301
Fragments arranged by size:
2301 1513 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4125 4210 5281 4125
Size: 85 1071 4908
Fragments arranged by size:
4908 1071 85
BsmI GAATG_Cn'
Cuts at: 3575 3668 3575
Size: 93 5971
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2804 3383 4125 4210 5281 1391
Size: 399 1014 579 742 85 1071 2174
Fragments arranged by size:
2174 1071 1014 742 579 399 85
BspEI T'CCGG_A
Cuts at: 5727 5727
Size: 6064
BspHI T'CATG_A
Cuts at: 3953 5683 3953
Size: 1730 4334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3073 3419 844
Size: 2229 346 3489
Fragments arranged by size:
3489 2229 346
BsrBI CCG'CTC
Cuts at: 1394 1823 3951 1394
Size: 429 2128 3507
Fragments arranged by size:
3507 2128 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4559 4733 65
Size: 1183 3311 174 1396
Fragments arranged by size:
3311 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4708 4895 1700
Size: 110 2898 187 2869
Fragments arranged by size:
2898 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6064
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6064
BssSI C'ACGA_G
Cuts at: 4124 5136 4124
Size: 1012 5052
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6064
BstBI TT'CG_AA
Cuts at: 2525 4868 2525
Size: 2343 3721
BstEII G'GTnAC_C
Cuts at: 1184 2365 3005 1184
Size: 1181 640 4243
Fragments arranged by size:
4243 1181 640
BstXI CCAn_nnnn'nTGG
Cuts at: 2950 2950
Size: 6064
BstYI r'GATC_y
Cuts at: 1972 2329 3394 3761 4146 4163 5615 5701 5713
Size: 357 1065 367 385 17 1452 86 12
Cuts at: 5713 6059 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1065 385 367 357 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2260 3010 3058 513
Size: 552 1195 750 48 3519
Fragments arranged by size:
3519 1195 750 552 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3211 487
Size: 683 56 1985 3340
Fragments arranged by size:
3340 1985 683 56
BtsI GCAGTG_nn'
Cuts at: 3580 4374 4394 3580
Size: 794 20 5250
Fragments arranged by size:
5250 794 20
ClaI AT'CG_AT
Cuts at: 1554 2769 1554
Size: 1215 4849
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6029
DraI TTT'AAA
Cuts at: 3368 3368
Size: 6064
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5071 809
Size: 507 3755 1802
Fragments arranged by size:
3755 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2578 3511 4400
Size: 54 1425 50 275 18 748 933 889
Cuts at: 4400 6029 8
Size: 1629 43
Fragments arranged by size:
1629 1425 933 889 748 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3511 1537
Size: 275 1699 4090
Fragments arranged by size:
4090 1699 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2068 2452 3994 1353
Size: 715 384 1542 3423
Fragments arranged by size:
3423 1542 715 384
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4651 5025 5171 387
Size: 1064 138 3062 374 146 1280
Fragments arranged by size:
3062 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2095 2135 2147 2363 2616 3311 4127
Size: 393 463 40 12 216 253 695 816
Cuts at: 4127 4724 5511 5779 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 816 787 695 597 463 393 268
253 216 40 12
EcoO109I rG'GnC_Cy
Cuts at: 3165 3165
Size: 6064
EcoRI G'AATT_C
Cuts at: 2934 3373 3751 2934
Size: 439 378 5247
Fragments arranged by size:
5247 439 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2079 2323 2079
Size: 244 5820
FspI TGC'GCA
Cuts at: 4570 4570
Size: 6064
HaeII r_GCGC'y
Cuts at: 1678 5211 1678
Size: 3533 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2051 2083 2429 2461 3157
Size: 32 135 32 479 32 346 32 696
Cuts at: 3157 3189 4705 4737 4779 4811 1373
Size: 32 1516 32 42 32 2626
Fragments arranged by size:
2626 1516 696 479 346 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3196 3258 3408 3654 669
Size: 988 281 1258 62 150 246 3079
Fragments arranged by size:
3079 1258 988 281 246 150 62
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6064
HpaI GTT'AAC
Cuts at: 3258 3654 3258
Size: 396 5668
KpnI G_GTAC'C
Cuts at: 3389 3389
Size: 6064
MfeI C'AATT_G
Cuts at: 2973 3663 2973
Size: 690 5374
MluI A'CGCG_T
Cuts at: 5940 5940
Size: 6064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5178 5362 1305
Size: 3873 184 2007
Fragments arranged by size:
3873 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2580 10
Size: 54 1425 343 748 3494
Fragments arranged by size:
3494 1425 748 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2877 3015 4022 4381 4540 518
Size: 648 1711 138 1007 359 159 2042
Fragments arranged by size:
2042 1711 1007 648 359 159 138
MspA1I CmG'CkG
Cuts at: 1606 3480 4157 5305 5550 1606
Size: 1874 677 1148 245 2120
Fragments arranged by size:
2120 1874 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2260 3010 513
Size: 552 1195 750 3567
Fragments arranged by size:
3567 1195 750 552
NdeI CA'TA_TG
Cuts at: 387 2874 387
Size: 2487 3577
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6064
NotI GC'GGCC_GC
Cuts at: 3511 3511
Size: 6064
NruI TCG'CGA
Cuts at: 3230 3230
Size: 6064
NsiI A_TGCA'T
Cuts at: 3042 3042
Size: 6064
NspI r_CATG'y
Cuts at: 1132 1229 2189 3273 3422 4967 1132
Size: 97 960 1084 149 1545 2229
Fragments arranged by size:
2229 1545 1084 960 149 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6064
PciI A'CATG_T
Cuts at: 4963 4963
Size: 6064
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3136 1108
Size: 2028 4036
PmeI GTTT'AAAC
Cuts at: 3368 3368
Size: 6064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4133 4165 5646 5678 4133
Size: 32 1481 32 4519
Fragments arranged by size:
4519 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3165 3165
Size: 6064
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6064
PsiI TTA'TAA
Cuts at: 2042 2847 3634 2042
Size: 805 787 4472
Fragments arranged by size:
4472 805 787
PstI C_TGCA'G
Cuts at: 830 3416 830
Size: 2586 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6064
SalI G'TCGA_C
Cuts at: 1936 3406 1936
Size: 1470 4594
SbfI CC_TGCA'GG
Cuts at: 3416 3416
Size: 6064
ScaI AGT'ACT
Cuts at: 4312 6024 4312
Size: 1712 4352
SfcI C'TryA_G
Cuts at: 826 1045 2227 2863 3412 4547 5228 5419 826
Size: 219 1182 636 549 1135 681 191 1471
Fragments arranged by size:
1471 1182 1135 681 636 549 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6064
SmaI CCC'GGG
Cuts at: 1872 3391 1872
Size: 1519 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2666 4168 5069 5331 820
Size: 197 926 723 1502 901 262 1553
Fragments arranged by size:
1553 1502 926 901 723 262 197
SnaBI TAC'GTA
Cuts at: 493 3855 493
Size: 3362 2702
SpeI A'CTAG_T
Cuts at: 152 2092 152
Size: 1940 4124
SphI G_CATG'C
Cuts at: 1132 3422 1132
Size: 2290 3774
SspI AAT'ATT
Cuts at: 5 52 2503 3988 5
Size: 47 2451 1485 2081
Fragments arranged by size:
2451 2081 1485 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6064
StyI C'CwwG_G
Cuts at: 513 1065 2260 3010 3503 513
Size: 552 1195 750 493 3074
Fragments arranged by size:
3074 1195 750 552 493
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2353 4091 4108 4261 4446 4891 325
Size: 1255 773 1738 17 153 185 445 1498
Fragments arranged by size:
1738 1498 1255 773 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4310 6022 96
Size: 274 80 33 51 1443 2333 1712 138
Fragments arranged by size:
2333 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6024 6056 6024
Size: 32 6032
Tth111I GACn'n_nGTC
Cuts at: 1316 2654 1316
Size: 1338 4726
XbaI T'CTAG_A
Cuts at: 3400 3400
Size: 6064
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6064
XmaI C'CCGG_G
Cuts at: 1870 3389 1870
Size: 1519 4545
XmnI GAAnn'nnTTC
Cuts at: 2898 4193 2898
Size: 1295 4769
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3872 276
Size: 53 83 186 936 2338 2468
Fragments arranged by size:
2468 2338 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NruI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI