Restriction map for
pFN21AA0878
(Circular) (Six-base) MAPSORT of: pFN21AA0878.seq Check: 6435 from: 1 to: 6538
pFN21AA0878 6538 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4348 278
Size: 53 83 186 936 2812 2468
Fragments arranged by size:
2812 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3881 1937
Size: 1944 4594
Acc65I G'GTAC_C
Cuts at: 2405 3859 2405
Size: 1454 5084
AclI AA'CG_TT
Cuts at: 1563 2307 4665 5038 1563
Size: 744 2358 373 3063
Fragments arranged by size:
3063 2358 744 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2502 2730 2952 3676 4601 5985 6253 1632
Size: 870 228 222 724 925 1384 268 1917
Fragments arranged by size:
1917 1384 925 870 724 268 228 222
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6341
AflIII A'CryG_T
Cuts at: 1203 5437 6414 1203
Size: 4234 977 1327
Fragments arranged by size:
4234 1327 977
AgeI A'CCGG_T
Cuts at: 5369 5369
Size: 6538
AhdI GACnn_n'nnGTC
Cuts at: 5267 5267
Size: 6538
AleI CACnn'nnGTG
Cuts at: 1166 3531 1166
Size: 2365 4173
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2147 1184
Size: 963 5575
AlwNI CAG_nnn'CTG
Cuts at: 4281 5853 4281
Size: 1572 4966
ApaLI G'TGCA_C
Cuts at: 4595 5751 4595
Size: 1156 5382
ApoI r'AATT_y
Cuts at: 1493 2374 3279 3707 3847 4073 4225 6334 1493
Size: 881 905 428 140 226 152 2109 1697
Fragments arranged by size:
2109 1697 905 881 428 226 152 140
AseI AT'TA_AT
Cuts at: 160 1032 3168 5092 160
Size: 872 2136 1924 1606
Fragments arranged by size:
2136 1924 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6538
AvaI C'yCGr_G
Cuts at: 1870 1943 3863 1870
Size: 73 1920 4545
Fragments arranged by size:
4545 1920 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3279 3312 3279
Size: 33 6505
BamHI G'GATC_C
Cuts at: 2487 3868 4235 2487
Size: 1381 367 4790
Fragments arranged by size:
4790 1381 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2405 3859 5314 618
Size: 325 217 627 618 1454 1455 1842
Fragments arranged by size:
1842 1455 1454 627 618 325 217
BanII G_rGCy'C
Cuts at: 1391 3857 1391
Size: 2466 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2615 3265 928
Size: 971 716 650 4201
Fragments arranged by size:
4201 971 716 650
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4729 4763 1903
Size: 34 2792 34 3678
Fragments arranged by size:
3678 2792 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4430 5640 4430
Size: 1210 5328
BclI T'GATC_A
Cuts at: 1548 2295 1548
Size: 747 5791
BfrBI ATG'CAT
Cuts at: 3144 3144
Size: 6538
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2071 3308 3462
Size: 107 122 71 631 605 399 1237 154
Cuts at: 3462 5149 136
Size: 1687 1525
Fragments arranged by size:
1687 1525 1237 631 605 399 154 122
107 71
BglII A'GATC_T
Cuts at: 6533 6533
Size: 6538
BlpI GC'TnA_GC
Cuts at: 3955 3955
Size: 6538
Bme1580I G_kGCm'C
Cuts at: 1790 4599 5755 1790
Size: 2809 1156 2573
Fragments arranged by size:
2809 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2770 1318
Size: 1452 5086
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4269 5227 442
Size: 416 53 83 399 24 2852 958 1753
Fragments arranged by size:
2852 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2958 1056
Size: 1902 4636
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2118 2118
Size: 6538
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5198 1239
Size: 3959 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3218 4663 5528 5826 3218
Size: 1445 865 298 3930
Fragments arranged by size:
3930 1445 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2113 5201 882
Size: 617 614 3088 2219
Fragments arranged by size:
3088 2219 617 614
BsaAI yAC'GTr
Cuts at: 493 1204 4329 493
Size: 711 3125 2702
Fragments arranged by size:
3125 2702 711
BsaBI GATnn'nnATC
Cuts at: 6532 6532
Size: 6538
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3003 4345 4727 5362
Size: 53 83 186 936 1470 1342 382 635
Cuts at: 5362 275
Size: 1451
Fragments arranged by size:
1470 1451 1342 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2161 4971 5369 5643 5790 6201 2161
Size: 2810 398 274 147 411 2498
Fragments arranged by size:
2810 2498 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3431 3461 1178
Size: 30 165 30 2028 30 4255
Fragments arranged by size:
4255 2028 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2583 1186
Size: 1397 5141
BseYI C'CCAG_C
Cuts at: 2026 3457 3552 5741 6237 2026
Size: 1431 95 2189 496 2327
Fragments arranged by size:
2327 2189 1431 496 95
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5965
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2066 2154 3988 4749 5777 1540
Size: 275 186 65 88 1834 761 1028 2301
Fragments arranged by size:
2301 1834 1028 761 275 186 88 65
BsiHKAI G_wGCw'C
Cuts at: 4599 4684 5755 4599
Size: 85 1071 5382
Fragments arranged by size:
5382 1071 85
BsmI GAATG_Cn'
Cuts at: 4049 4142 4049
Size: 93 6445
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3857 4599 4684 5755 1391
Size: 399 2067 742 85 1071 2174
Fragments arranged by size:
2174 2067 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6201 6201
Size: 6538
BspHI T'CATG_A
Cuts at: 4427 6157 4427
Size: 1730 4808
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3618 3893 844
Size: 2774 275 3489
Fragments arranged by size:
3489 2774 275
BsrBI CCG'CTC
Cuts at: 1394 1823 4425 1394
Size: 429 2602 3507
Fragments arranged by size:
3507 2602 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3669 5033 5207 65
Size: 1183 2421 1364 174 1396
Fragments arranged by size:
2421 1396 1364 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2061 5182 5369 1700
Size: 110 251 3121 187 2869
Fragments arranged by size:
3121 2869 251 187 110
BsrGI T'GTAC_A
Cuts at: 96 2432 3438 96
Size: 2336 1006 3196
Fragments arranged by size:
3196 2336 1006
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6538
BssSI C'ACGA_G
Cuts at: 4598 5610 4598
Size: 1012 5526
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3565 794
Size: 2771 3767
BstBI TT'CG_AA
Cuts at: 3276 5342 3276
Size: 2066 4472
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6538
BstXI CCAn_nnnn'nTGG
Cuts at: 3319 3319
Size: 6538
BstYI r'GATC_y
Cuts at: 1972 2487 2914 3715 3868 4235 4620 4637 6089
Size: 515 427 801 153 367 385 17 1452
Cuts at: 6089 6175 6187 6533 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 801 515 427 385 367 346
153 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3176 3176
Size: 6538
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5986
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5799
Fragments arranged by size:
5799 683 56
BtsI GCAGTG_nn'
Cuts at: 4054 4848 4868 4054
Size: 794 20 5724
Fragments arranged by size:
5724 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6538
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3623 3658 529
Size: 35 3059 35 3409
Fragments arranged by size:
3409 3059 35 35
DraI TTT'AAA
Cuts at: 2793 3150 3842 2793
Size: 357 692 5489
Fragments arranged by size:
5489 692 357
DraIII CAC_nnn'GTG
Cuts at: 2020 2020
Size: 6538
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2225 3418 5545 809
Size: 507 909 1193 2127 1802
Fragments arranged by size:
2127 1802 1193 909 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2063 2142 3758
Size: 54 1425 50 275 18 233 79 1616
Cuts at: 3758 3985 4874 6503 8
Size: 227 889 1629 43
Fragments arranged by size:
1629 1616 1425 889 275 233 227 79
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2063 3985 1537
Size: 275 251 1922 4090
Fragments arranged by size:
4090 1922 275 251
EarI CTCTTCn'nnn_
Cuts at: 1353 2415 2861 3014 4468 1353
Size: 1062 446 153 1454 3423
Fragments arranged by size:
3423 1454 1062 446 153
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5125 5499 5645 387
Size: 1064 138 3536 374 146 1280
Fragments arranged by size:
3536 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2502 2730 2952 3676 4601 5198 5985
Size: 393 870 228 222 724 925 597 787
Cuts at: 5985 6253 1239
Size: 268 1524
Fragments arranged by size:
1524 925 870 787 724 597 393 268
228 222
EcoRI G'AATT_C
Cuts at: 3847 4225 3847
Size: 378 6160
EcoRV GAT'ATC
Cuts at: 3085 3085
Size: 6538
FspI TGC'GCA
Cuts at: 5044 5044
Size: 6538
HaeII r_GCGC'y
Cuts at: 1678 2027 2283 5685 1678
Size: 349 256 3402 2531
Fragments arranged by size:
3402 2531 349 256
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5179 5211 5253 5285 1373
Size: 32 135 32 3607 32 42 32 2626
Fragments arranged by size:
3607 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3607 3882 4128 669
Size: 988 281 1669 275 246 3079
Fragments arranged by size:
3079 1669 988 281 275 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6538
HpaI GTT'AAC
Cuts at: 4128 4128
Size: 6538
KpnI G_GTAC'C
Cuts at: 2409 3863 2409
Size: 1454 5084
MfeI C'AATT_G
Cuts at: 4137 4137
Size: 6538
MluI A'CGCG_T
Cuts at: 6414 6414
Size: 6538
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2515 2556 5652 5836 1305
Size: 1210 41 3096 184 2007
Fragments arranged by size:
3096 2007 1210 184 41
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4716
Fragments arranged by size:
4716 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2291 3317 3531 4496 4855 5014 518
Size: 648 1125 1026 214 965 359 159 2042
Fragments arranged by size:
2042 1125 1026 965 648 359 214 159
MspA1I CmG'CkG
Cuts at: 1606 3461 3556 3954 4631 5779 6024 1606
Size: 1855 95 398 677 1148 245 2120
Fragments arranged by size:
2120 1855 1148 677 398 245 95
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5986
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6538
NheI G'CTAG_C
Cuts at: 1052 2954 1052
Size: 1902 4636
NotI GC'GGCC_GC
Cuts at: 3985 3985
Size: 6538
NsiI A_TGCA'T
Cuts at: 3146 3146
Size: 6538
NspI r_CATG'y
Cuts at: 1132 1229 2439 3568 3896 5441 1132
Size: 97 1210 1129 328 1545 2229
Fragments arranged by size:
2229 1545 1210 1129 328 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6538
PciI A'CATG_T
Cuts at: 5437 5437
Size: 6538
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2020 2764 1108
Size: 912 744 4882
Fragments arranged by size:
4882 912 744
PfoI T'CCnGG_A
Cuts at: 3812 3812
Size: 6538
PmeI GTTT'AAAC
Cuts at: 3842 3842
Size: 6538
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4607 4639 6120 6152 4607
Size: 32 1481 32 4993
Fragments arranged by size:
4993 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6538
PsiI TTA'TAA
Cuts at: 2724 2781 3222 4108 2724
Size: 57 441 886 5154
Fragments arranged by size:
5154 886 441 57
PstI C_TGCA'G
Cuts at: 830 3525 3561 3890 830
Size: 2695 36 329 3478
Fragments arranged by size:
3478 2695 329 36
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6538
PvuII CAG'CTG
Cuts at: 3461 3556 3461
Size: 95 6443
SalI G'TCGA_C
Cuts at: 1936 3880 1936
Size: 1944 4594
SbfI CC_TGCA'GG
Cuts at: 3890 3890
Size: 6538
ScaI AGT'ACT
Cuts at: 4786 6498 4786
Size: 1712 4826
SfcI C'TryA_G
Cuts at: 826 1045 3521 3557 3886 5021 5702 5893 826
Size: 219 2476 36 329 1135 681 191 1471
Fragments arranged by size:
2476 1471 1135 681 329 219 191 36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6538
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6538
SmaI CCC'GGG
Cuts at: 1872 3865 1872
Size: 1993 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3233 4642 5543 5805 820
Size: 197 926 1290 1409 901 262 1553
Fragments arranged by size:
1553 1409 1290 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4329 493
Size: 3836 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6538
SphI G_CATG'C
Cuts at: 1132 3568 3896 1132
Size: 2436 328 3774
Fragments arranged by size:
3774 2436 328
SspI AAT'ATT
Cuts at: 5 52 4462 5
Size: 47 4410 2081
Fragments arranged by size:
4410 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6538
StyI C'CwwG_G
Cuts at: 513 1065 3977 513
Size: 552 2912 3074
Fragments arranged by size:
3074 2912 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4565 4582 4735 4920 5365 325
Size: 1255 2985 17 153 185 445 1498
Fragments arranged by size:
2985 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2432 2474 3438
Size: 274 80 33 51 1443 455 42 964
Cuts at: 3438 3771 4784 6496 96
Size: 333 1013 1712 138
Fragments arranged by size:
1712 1443 1013 964 455 333 274 138
80 51 42 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6498 6530 6498
Size: 32 6506
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6538
XbaI T'CTAG_A
Cuts at: 3874 3874
Size: 6538
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2017 2017
Size: 6538
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6538
XmaI C'CCGG_G
Cuts at: 1870 3863 1870
Size: 1993 4545
XmnI GAAnn'nnTTC
Cuts at: 4667 4667
Size: 6538
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4346 276
Size: 53 83 186 936 2812 2468
Fragments arranged by size:
2812 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoO109I FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI