Restriction map for
pFN21AA0877
(Circular) (Six-base) MAPSORT of: pFN21AA0877.seq Check: 1094 from: 1 to: 6361
pFN21AA0877 6361 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4171 278
Size: 53 83 186 936 2635 2468
Fragments arranged by size:
2635 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2801 3399 3704 1937
Size: 864 598 305 4594
Fragments arranged by size:
4594 864 598 305
Acc65I G'GTAC_C
Cuts at: 3682 3682
Size: 6361
AclI AA'CG_TT
Cuts at: 1563 4488 4861 1563
Size: 2925 373 3063
Fragments arranged by size:
3063 2925 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2285 4424 5808 6076 1632
Size: 653 2139 1384 268 1917
Fragments arranged by size:
2139 1917 1384 653 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6164
AflIII A'CryG_T
Cuts at: 1203 2953 3069 5260 6237 1203
Size: 1750 116 2191 977 1327
Fragments arranged by size:
2191 1750 1327 977 116
AgeI A'CCGG_T
Cuts at: 5192 5192
Size: 6361
AhdI GACnn_n'nnGTC
Cuts at: 5090 5090
Size: 6361
AleI CACnn'nnGTG
Cuts at: 1166 3070 3072 1166
Size: 1904 2 4455
Fragments arranged by size:
4455 1904 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6361
AlwNI CAG_nnn'CTG
Cuts at: 4104 5676 4104
Size: 1572 4789
ApaLI G'TGCA_C
Cuts at: 4418 5574 4418
Size: 1156 5205
ApoI r'AATT_y
Cuts at: 1493 2715 2765 3528 3670 3896 4048 6157 1493
Size: 1222 50 763 142 226 152 2109 1697
Fragments arranged by size:
2109 1697 1222 763 226 152 142 50
AseI AT'TA_AT
Cuts at: 160 1032 4915 160
Size: 872 3883 1606
Fragments arranged by size:
3883 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6361
AvaI C'yCGr_G
Cuts at: 1870 1943 3686 1870
Size: 73 1743 4545
Fragments arranged by size:
4545 1743 73
BamHI G'GATC_C
Cuts at: 3691 4058 3691
Size: 367 5994
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3296 3682 5137 618
Size: 325 217 627 1509 386 1455 1842
Fragments arranged by size:
1842 1509 1455 627 386 325 217
BanII G_rGCy'C
Cuts at: 1391 3680 1391
Size: 2289 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3372 928
Size: 971 1473 3917
Fragments arranged by size:
3917 1473 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4552 4586 1903
Size: 34 2615 34 3678
Fragments arranged by size:
3678 2615 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4253 5463 4253
Size: 1210 5151
BclI T'GATC_A
Cuts at: 1548 2974 1548
Size: 1426 4935
BfrBI ATG'CAT
Cuts at: 2999 2999
Size: 6361
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4972 136
Size: 107 122 71 631 605 3300 1525
Fragments arranged by size:
3300 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6356 6356
Size: 6361
BlpI GC'TnA_GC
Cuts at: 3778 3778
Size: 6361
Bme1580I G_kGCm'C
Cuts at: 1790 4422 5578 1790
Size: 2632 1156 2573
Fragments arranged by size:
2632 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6361
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3196 4092 5050
Size: 416 53 83 399 24 1779 896 958
Cuts at: 5050 442
Size: 1753
Fragments arranged by size:
1779 1753 958 896 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6361
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2078 2078
Size: 6361
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5021 1239
Size: 3782 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3082 3397 4486 5351 5649 3082
Size: 315 1089 865 298 3794
Fragments arranged by size:
3794 1089 865 315 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5024 882
Size: 617 3525 2219
Fragments arranged by size:
3525 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4152 493
Size: 711 2948 2702
Fragments arranged by size:
2948 2702 711
BsaBI GATnn'nnATC
Cuts at: 6355 6355
Size: 6361
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4168 4550 5185 275
Size: 53 83 186 936 2635 382 635 1451
Fragments arranged by size:
2635 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4794 5192 5466 5613 6024 4794
Size: 398 274 147 411 5131
Fragments arranged by size:
5131 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2187 2217 2250 2280 2973
Size: 30 165 30 784 30 33 30 693
Cuts at: 2973 3003 1178
Size: 30 4536
Fragments arranged by size:
4536 784 693 165 33 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2067 1186
Size: 881 5480
BseYI C'CCAG_C
Cuts at: 3557 5564 6060 3557
Size: 2007 496 3858
Fragments arranged by size:
3858 2007 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2829 3315 1163
Size: 573 1093 486 4209
Fragments arranged by size:
4209 1093 573 486
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3811 4572 5600 1540
Size: 275 186 1810 761 1028 2301
Fragments arranged by size:
2301 1810 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4422 4507 5578 4422
Size: 85 1071 5205
Fragments arranged by size:
5205 1071 85
BsmI GAATG_Cn'
Cuts at: 3872 3965 3872
Size: 93 6268
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3680 4422 4507 5578 1391
Size: 399 1890 742 85 1071 2174
Fragments arranged by size:
2174 1890 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6024 6024
Size: 6361
BspHI T'CATG_A
Cuts at: 4250 5980 4250
Size: 1730 4631
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3716 844
Size: 2872 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4248 1394
Size: 429 2425 3507
Fragments arranged by size:
3507 2425 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2613 2999 3174 4856 5030 65
Size: 1183 1365 386 175 1682 174 1396
Fragments arranged by size:
1682 1396 1365 1183 386 175 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5005 5192 1700
Size: 110 3195 187 2869
Fragments arranged by size:
3195 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6361
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6361
BssSI C'ACGA_G
Cuts at: 4421 5433 4421
Size: 1012 5349
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3290 794
Size: 2496 3865
BstBI TT'CG_AA
Cuts at: 5165 5165
Size: 6361
BstEII G'GTnAC_C
Cuts at: 1184 2044 1184
Size: 860 5501
BstYI r'GATC_y
Cuts at: 1972 2095 3500 3544 3691 4058 4443 4460 5912
Size: 123 1405 44 147 367 385 17 1452
Cuts at: 5912 5998 6010 6356 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1405 385 367 346 147 123
86 44 17 12
BstZ17I GTA'TAC
Cuts at: 2802 3400 2802
Size: 598 5763
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4870
Fragments arranged by size:
4870 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5622
Fragments arranged by size:
5622 683 56
BtsI GCAGTG_nn'
Cuts at: 3291 3877 4671 4691 3291
Size: 586 794 20 4961
Fragments arranged by size:
4961 794 586 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6361
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3185 3220 529
Size: 35 2621 35 3670
Fragments arranged by size:
3670 2621 35 35
DraI TTT'AAA
Cuts at: 2133 3665 2133
Size: 1532 4829
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5368 809
Size: 507 4052 1802
Fragments arranged by size:
4052 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3808 4697 6326
Size: 54 1425 50 275 18 1978 889 1629
Cuts at: 6326 8
Size: 43
Fragments arranged by size:
1978 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3808 1537
Size: 275 1996 4090
Fragments arranged by size:
4090 1996 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3059 3474 4291 1353
Size: 1706 415 817 3423
Fragments arranged by size:
3423 1706 817 415
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4948 5322 5468 387
Size: 1064 138 3359 374 146 1280
Fragments arranged by size:
3359 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2285 4424 5021 5808 6076 1239
Size: 393 653 2139 597 787 268 1524
Fragments arranged by size:
2139 1524 787 653 597 393 268
EcoRI G'AATT_C
Cuts at: 3670 4048 3670
Size: 378 5983
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3263 3263
Size: 6361
FspI TGC'GCA
Cuts at: 4867 4867
Size: 6361
HaeII r_GCGC'y
Cuts at: 1678 5508 1678
Size: 3830 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2248 2280 3036 3068 5002
Size: 32 135 32 676 32 756 32 1934
Cuts at: 5002 5034 5076 5108 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1934 756 676 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3705 3951 669
Size: 988 281 1767 246 3079
Fragments arranged by size:
3079 1767 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2275 2353 748
Size: 1527 78 4756
Fragments arranged by size:
4756 1527 78
HpaI GTT'AAC
Cuts at: 3951 3951
Size: 6361
KpnI G_GTAC'C
Cuts at: 3686 3686
Size: 6361
MfeI C'AATT_G
Cuts at: 3005 3960 3005
Size: 955 5406
MluI A'CGCG_T
Cuts at: 6237 6237
Size: 6361
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2018 3395 5475 5659 1305
Size: 713 1377 2080 184 2007
Fragments arranged by size:
2080 2007 1377 713 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4539
Fragments arranged by size:
4539 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3070 3072 3138 4319 4678 4837 518
Size: 648 1904 2 66 1181 359 159 2042
Fragments arranged by size:
2042 1904 1181 648 359 159 66 2
MspA1I CmG'CkG
Cuts at: 1606 2813 3777 4454 5602 5847 1606
Size: 1207 964 677 1148 245 2120
Fragments arranged by size:
2120 1207 1148 964 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4870
Fragments arranged by size:
4870 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6361
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6361
NotI GC'GGCC_GC
Cuts at: 3808 3808
Size: 6361
NsiI A_TGCA'T
Cuts at: 3001 3001
Size: 6361
NspI r_CATG'y
Cuts at: 1132 1229 2957 3073 3719 5264 1132
Size: 97 1728 116 646 1545 2229
Fragments arranged by size:
2229 1728 1545 646 116 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6361
PciI A'CATG_T
Cuts at: 2953 3069 5260 2953
Size: 116 2191 4054
Fragments arranged by size:
4054 2191 116
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6361
PmeI GTTT'AAAC
Cuts at: 3665 3665
Size: 6361
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4430 4462 5943 5975 4430
Size: 32 1481 32 4816
Fragments arranged by size:
4816 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6361
PsiI TTA'TAA
Cuts at: 2778 3931 2778
Size: 1153 5208
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3617 3649 3617
Size: 32 6329
PstI C_TGCA'G
Cuts at: 830 3713 830
Size: 2883 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6361
SalI G'TCGA_C
Cuts at: 1936 3703 1936
Size: 1767 4594
SbfI CC_TGCA'GG
Cuts at: 3713 3713
Size: 6361
ScaI AGT'ACT
Cuts at: 3271 4609 6321 3271
Size: 1338 1712 3311
Fragments arranged by size:
3311 1712 1338
SfcI C'TryA_G
Cuts at: 826 1045 3709 4844 5525 5716 826
Size: 219 2664 1135 681 191 1471
Fragments arranged by size:
2664 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6361
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6361
SmaI CCC'GGG
Cuts at: 1872 3688 1872
Size: 1816 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3097 3376 4465 5366 5628 820
Size: 197 926 1154 279 1089 901 262 1553
Fragments arranged by size:
1553 1154 1089 926 901 279 262 197
SnaBI TAC'GTA
Cuts at: 493 4152 493
Size: 3659 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6361
SphI G_CATG'C
Cuts at: 1132 3719 1132
Size: 2587 3774
SspI AAT'ATT
Cuts at: 5 52 3211 4285 5
Size: 47 3159 1074 2081
Fragments arranged by size:
3159 2081 1074 47
StuI AGG'CCT
Cuts at: 1517 2199 1517
Size: 682 5679
StyI C'CwwG_G
Cuts at: 513 1065 2004 2396 3025 3800 513
Size: 552 939 392 629 775 3074
Fragments arranged by size:
3074 939 775 629 552 392
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4388 4405 4558 4743 5188 325
Size: 1255 2808 17 153 185 445 1498
Fragments arranged by size:
2808 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2416 3130 3269
Size: 274 80 33 51 1443 439 714 139
Cuts at: 3269 4607 6319 96
Size: 1338 1712 138
Fragments arranged by size:
1712 1443 1338 714 439 274 139 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2250 2282 6321 6353 2250
Size: 32 4039 32 2258
Fragments arranged by size:
4039 2258 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2064 1316
Size: 748 5613
XbaI T'CTAG_A
Cuts at: 3475 3697 3475
Size: 222 6139
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2975 3307 2975
Size: 332 6029
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6361
XmaI C'CCGG_G
Cuts at: 1870 3686 1870
Size: 1816 4545
XmnI GAAnn'nnTTC
Cuts at: 4490 4490
Size: 6361
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4169 276
Size: 53 83 186 936 2635 2468
Fragments arranged by size:
2635 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI