Restriction map for
pFN21AA0868
(Circular) (Six-base) MAPSORT of: pFN21AA0868.seq Check: 2090 from: 1 to: 8698
pFN21AA0868 8698 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3779 4305 3779
Size: 526 8172
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2660 6508 278
Size: 53 83 186 936 1124 3848 2468
Fragments arranged by size:
3848 2468 1124 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5103 5310 6041 1937
Size: 3166 207 731 4594
Fragments arranged by size:
4594 3166 731 207
Acc65I G'GTAC_C
Cuts at: 6019 6019
Size: 8698
AclI AA'CG_TT
Cuts at: 1563 6825 7198 1563
Size: 5262 373 3063
Fragments arranged by size:
5262 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2243 3223 3527 4574 5146 5152 6761 8145
Size: 611 980 304 1047 572 6 1609 1384
Cuts at: 8145 8413 1632
Size: 268 1917
Fragments arranged by size:
1917 1609 1384 1047 980 611 572 304
268 6
AfeI AGC'GCT
Cuts at: 2040 4465 2040
Size: 2425 6273
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8501
AflIII A'CryG_T
Cuts at: 1203 3654 5859 7597 8574 1203
Size: 2451 2205 1738 977 1327
Fragments arranged by size:
2451 2205 1738 1327 977
AgeI A'CCGG_T
Cuts at: 7529 7529
Size: 8698
AhdI GACnn_n'nnGTC
Cuts at: 7427 7427
Size: 8698
AleI CACnn'nnGTG
Cuts at: 1166 2795 4568 1166
Size: 1629 1773 5296
Fragments arranged by size:
5296 1773 1629
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2307 1184
Size: 1123 7575
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3445 3477 3445
Size: 32 8666
AlwNI CAG_nnn'CTG
Cuts at: 2064 2079 3184 3356 6441 8013 2064
Size: 15 1105 172 3085 1572 2749
Fragments arranged by size:
3085 2749 1572 1105 172 15
ApaLI G'TGCA_C
Cuts at: 5833 6755 7911 5833
Size: 922 1156 6620
Fragments arranged by size:
6620 1156 922
ApoI r'AATT_y
Cuts at: 1493 2998 3091 3406 3598 6007 6233 6385 8494
Size: 1505 93 315 192 2409 226 152 2109
Cuts at: 8494 1493
Size: 1697
Fragments arranged by size:
2409 2109 1697 1505 315 226 192 152
93
AseI AT'TA_AT
Cuts at: 160 1032 7252 160
Size: 872 6220 1606
Fragments arranged by size:
6220 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8698
AvaI C'yCGr_G
Cuts at: 1870 1943 4894 5286 6023 1870
Size: 73 2951 392 737 4545
Fragments arranged by size:
4545 2951 737 392 73
AvrII C'CTAG_G
Cuts at: 3817 3817
Size: 8698
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2887 2920 4945 4978 2887
Size: 33 2025 33 6607
Fragments arranged by size:
6607 2025 33 33
BamHI G'GATC_C
Cuts at: 6028 6395 6028
Size: 367 8331
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3124 3160 4650 5065 5182
Size: 325 217 627 1337 36 1490 415 117
Cuts at: 5182 5282 5697 5876 6019 7474 618
Size: 100 415 179 143 1455 1842
Fragments arranged by size:
1842 1490 1455 1337 627 415 415 325
217 179 143 117 100 36
BanII G_rGCy'C
Cuts at: 1391 3078 4758 6017 1391
Size: 1687 1680 1259 4072
Fragments arranged by size:
4072 1687 1680 1259
BbeI G_GCGC'C
Cuts at: 5186 5186
Size: 8698
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3229 5375 5836 928
Size: 971 1330 2146 461 3790
Fragments arranged by size:
3790 2146 1330 971 461
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3368 3402 6889 6923 1903
Size: 34 50 34 1347 34 3487 34 3678
Fragments arranged by size:
3678 3487 1347 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3749 6590 7800 3749
Size: 2841 1210 4647
Fragments arranged by size:
4647 2841 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8698
BfrBI ATG'CAT
Cuts at: 3564 3564
Size: 8698
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2084 3169
Size: 107 122 71 631 605 337 75 1085
Cuts at: 3169 4797 5874 7309 136
Size: 1628 1077 1435 1525
Fragments arranged by size:
1628 1525 1435 1085 1077 631 605 337
122 107 75 71
BglII A'GATC_T
Cuts at: 8693 8693
Size: 8698
BlpI GC'TnA_GC
Cuts at: 6115 6115
Size: 8698
Bme1580I G_kGCm'C
Cuts at: 1790 3165 3684 5837 5879 6759 7915 1790
Size: 1375 519 2153 42 880 1156 2573
Fragments arranged by size:
2573 2153 1375 1156 880 519 42
BmgBI CAC'GTC
Cuts at: 1318 2097 5611 1318
Size: 779 3514 4405
Fragments arranged by size:
4405 3514 779
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2563 4165 6429
Size: 416 53 83 399 24 1146 1602 2264
Cuts at: 6429 7387 442
Size: 958 1753
Fragments arranged by size:
2264 1753 1602 1146 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2968 3176 4568 4772 4871 5074 5527 7358
Size: 1729 208 1392 204 99 203 453 1831
Cuts at: 7358 1239
Size: 2579
Fragments arranged by size:
2579 1831 1729 1392 453 208 204 203
99
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3511 4843 5366 5558 5605 6823 7688 7986 3511
Size: 1332 523 192 47 1218 865 298 4223
Fragments arranged by size:
4223 1332 1218 865 523 298 192 47
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2232 3233 5957 7361 882
Size: 617 733 1001 2724 1404 2219
Fragments arranged by size:
2724 2219 1404 1001 733 617
BsaAI yAC'GTr
Cuts at: 493 1204 6489 493
Size: 711 5285 2702
Fragments arranged by size:
5285 2702 711
BsaBI GATnn'nnATC
Cuts at: 5394 8692 5394
Size: 3298 5400
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2657 3036 3947 5183
Size: 53 83 186 936 1124 379 911 1236
Cuts at: 5183 5802 5928 6505 6887 7522 275
Size: 619 126 577 382 635 1451
Fragments arranged by size:
1451 1236 1124 936 911 635 619 577
382 379 186 126 83 53
BsaWI w'CCGG_w
Cuts at: 2343 4886 5853 7131 7529 7803 7950 8361 2343
Size: 2543 967 1278 398 274 147 411 2680
Fragments arranged by size:
2680 2543 1278 967 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3989 4019 4409 4439 4541
Size: 30 165 30 2586 30 390 30 102
Cuts at: 4541 4571 4949 4979 5192 5222 1178
Size: 30 378 30 213 30 4654
Fragments arranged by size:
4654 2586 390 378 213 165 102 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2146 2149 2223 5165 5186 1186
Size: 960 3 74 2942 21 4698
Fragments arranged by size:
4698 2942 960 74 21 3
BseYI C'CCAG_C
Cuts at: 2216 2978 4784 7901 8397 2216
Size: 762 1806 3117 496 2517
Fragments arranged by size:
3117 2517 1806 762 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2666 4195 4418 1163
Size: 573 930 1529 223 5443
Fragments arranged by size:
5443 1529 930 573 223
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6148 6909 7937 1540
Size: 275 186 4147 761 1028 2301
Fragments arranged by size:
4147 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3713 4758 5837 6759 6844 7915 3713
Size: 1045 1079 922 85 1071 4496
Fragments arranged by size:
4496 1079 1071 1045 922 85
BsmI GAATG_Cn'
Cuts at: 2833 4405 5190 6209 6302 2833
Size: 1572 785 1019 93 5229
Fragments arranged by size:
5229 1572 1019 785 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3078 3165 3684 3713 4758 5837 5879
Size: 399 1288 87 519 29 1045 1079 42
Cuts at: 5879 6017 6759 6844 7915 1391
Size: 138 742 85 1071 2174
Fragments arranged by size:
2174 1288 1079 1071 1045 742 519 399
138 87 85 42 29
BspEI T'CCGG_A
Cuts at: 4886 8361 4886
Size: 3475 5223
BspHI T'CATG_A
Cuts at: 3928 5937 6587 8317 3928
Size: 2009 650 1730 4309
Fragments arranged by size:
4309 2009 1730 650
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3779 4305 4440 6053 844
Size: 2935 526 135 1613 3489
Fragments arranged by size:
3489 2935 1613 526 135
BsrBI CCG'CTC
Cuts at: 1394 1823 4818 6585 1394
Size: 429 2995 1767 3507
Fragments arranged by size:
3507 2995 1767 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2295 2637 3271 4262 4900 5805 7193
Size: 1183 1047 342 634 991 638 905 1388
Cuts at: 7193 7367 65
Size: 174 1396
Fragments arranged by size:
1396 1388 1183 1047 991 905 638 634
342 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2024 4701 7342 7529 1700
Size: 110 214 2677 2641 187 2869
Fragments arranged by size:
2869 2677 2641 214 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8698
BssHII G'CGCG_C
Cuts at: 1925 2020 1925
Size: 95 8603
BssSI C'ACGA_G
Cuts at: 3380 5363 6758 7770 3380
Size: 1983 1395 1012 4308
Fragments arranged by size:
4308 1983 1395 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5078 794
Size: 4284 4414
BstBI TT'CG_AA
Cuts at: 3451 7502 3451
Size: 4051 4647
BstEII G'GTnAC_C
Cuts at: 1184 2332 4620 1184
Size: 1148 2288 5262
Fragments arranged by size:
5262 2288 1148
BstYI r'GATC_y
Cuts at: 1972 3847 4216 4716 5154 6028 6395 6780 6797
Size: 1875 369 500 438 874 367 385 17
Cuts at: 6797 8249 8335 8347 8693 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1875 1452 874 500 438 385 369
367 346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3349 3349
Size: 8698
BtgI C'CryG_G
Cuts at: 513 1065 4008 4957 513
Size: 552 2943 949 4254
Fragments arranged by size:
4254 2943 949 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7959
Fragments arranged by size:
7959 683 56
BtsI GCAGTG_nn'
Cuts at: 4659 6214 7008 7028 4659
Size: 1555 794 20 6329
Fragments arranged by size:
6329 1555 794 20
ClaI AT'CG_AT
Cuts at: 1554 3339 3426 1554
Size: 1785 87 6826
Fragments arranged by size:
6826 1785 87
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5260 5295 529
Size: 35 4696 35 3932
Fragments arranged by size:
4696 3932 35 35
DraI TTT'AAA
Cuts at: 6002 6002
Size: 8698
DraIII CAC_nnn'GTG
Cuts at: 4122 4122
Size: 8698
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2438 3922 7705 809
Size: 507 1122 1484 3783 1802
Fragments arranged by size:
3783 1802 1484 1122 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2578 2785 5325
Size: 54 1425 50 275 18 748 207 2540
Cuts at: 5325 6145 7034 8663 8
Size: 820 889 1629 43
Fragments arranged by size:
2540 1629 1425 889 820 748 275 207
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6145 1537
Size: 275 4333 4090
Fragments arranged by size:
4333 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5444 6628 1353
Size: 4091 1184 3423
Fragments arranged by size:
4091 3423 1184
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2875 5671 5855 5921 7285 7659
Size: 1064 138 1286 2796 184 66 1364 374
Cuts at: 7659 7805 387
Size: 146 1280
Fragments arranged by size:
2796 1364 1286 1280 1064 374 184 146
138 66
EcoICRI GAG'CTC
Cuts at: 4756 4756
Size: 8698
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2243 2968 3176 3223 3527 4568 4574
Size: 393 611 725 208 47 304 1041 6
Cuts at: 4574 4772 4871 5074 5146 5152 5527 6761 7358
Size: 198 99 203 72 6 375 1234 597
Cuts at: 7358 8145 8413 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1234 1041 787 725 611 597 393
375 304 268 208 203 198 99 72
47 6 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3398 5144 3398
Size: 1746 6952
EcoO109I rG'GnC_Cy
Cuts at: 3181 3872 4364 4726 5649 3181
Size: 691 492 362 923 6230
Fragments arranged by size:
6230 923 691 492 362
EcoRI G'AATT_C
Cuts at: 6007 6385 6007
Size: 378 8320
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5392 5476 5599 5392
Size: 84 123 8491
Fragments arranged by size:
8491 123 84
FspI TGC'GCA
Cuts at: 2483 7204 2483
Size: 4721 3977
FspAI rTGC'GCAy
Cuts at: 2483 2483
Size: 8698
HaeII r_GCGC'y
Cuts at: 1678 2042 2854 4005 4467 5186 7845 1678
Size: 364 812 1151 462 719 2659 2531
Fragments arranged by size:
2659 2531 1151 812 719 462 364
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3173 3205 3332 3364 3425
Size: 32 135 32 1601 32 127 32 61
Cuts at: 3425 3457 4348 4380 4541 4573 4851 4883 5398
Size: 32 891 32 161 32 278 32 515
Cuts at: 5398 5430 5554 5586 7339 7371 7413 7445 1373
Size: 32 124 32 1753 32 42 32 2626
Fragments arranged by size:
2626 1753 1601 891 515 278 161 135
127 124 61 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2266 3040 5350 5776 6042 6288
Size: 988 281 328 774 2310 426 266 246
Cuts at: 6288 669
Size: 3079
Fragments arranged by size:
3079 2310 988 774 426 328 281 266
246
HindIII A'AGCT_T
Cuts at: 748 5254 748
Size: 4506 4192
HpaI GTT'AAC
Cuts at: 6288 6288
Size: 8698
KasI G'GCGC_C
Cuts at: 5182 5182
Size: 8698
KpnI G_GTAC'C
Cuts at: 6023 6023
Size: 8698
MfeI C'AATT_G
Cuts at: 6297 6297
Size: 8698
MluI A'CGCG_T
Cuts at: 8574 8574
Size: 8698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4300 5454 5664 7812 7996 1305
Size: 2995 1154 210 2148 184 2007
Fragments arranged by size:
2995 2148 2007 1154 210 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2580 2787 5327 10
Size: 54 1425 343 748 207 2540 3381
Fragments arranged by size:
3381 2540 1425 748 343 207 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2391 2795 3578 3653 4568 5810 6656
Size: 648 1225 404 783 75 915 1242 846
Cuts at: 6656 7015 7174 518
Size: 359 159 2042
Fragments arranged by size:
2042 1242 1225 915 846 783 648 404
359 159 75
MspA1I CmG'CkG
Cuts at: 1606 2064 3861 5659 6114 6791 7939 8184 1606
Size: 458 1797 1798 455 677 1148 245 2120
Fragments arranged by size:
2120 1798 1797 1148 677 458 455 245
NaeI GCC'GGC
Cuts at: 2026 2026
Size: 8698
NarI GG'CG_CC
Cuts at: 5183 5183
Size: 8698
NcoI C'CATG_G
Cuts at: 513 1065 4008 513
Size: 552 2943 5203
Fragments arranged by size:
5203 2943 552
NdeI CA'TA_TG
Cuts at: 387 4990 387
Size: 4603 4095
NgoMIV G'CCGG_C
Cuts at: 2024 2024
Size: 8698
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8698
NotI GC'GGCC_GC
Cuts at: 6145 6145
Size: 8698
NruI TCG'CGA
Cuts at: 3638 3638
Size: 8698
NsiI A_TGCA'T
Cuts at: 3566 3566
Size: 8698
NspI r_CATG'y
Cuts at: 1132 1229 2893 3568 3658 5863 6056 7601 1132
Size: 97 1664 675 90 2205 193 1545 2229
Fragments arranged by size:
2229 2205 1664 1545 675 193 97 90
PasI CC'CwG_GG
Cuts at: 1348 4500 1348
Size: 3152 5546
PciI A'CATG_T
Cuts at: 3654 5859 7597 3654
Size: 2205 1738 4755
Fragments arranged by size:
4755 2205 1738
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4315 1108
Size: 3207 5491
PfoI T'CCnGG_A
Cuts at: 2413 5743 2413
Size: 3330 5368
PmeI GTTT'AAAC
Cuts at: 6002 6002
Size: 8698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6767 6799 8280 8312 6767
Size: 32 1481 32 7153
Fragments arranged by size:
7153 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3181 4364 3181
Size: 1183 7515
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8698
PsiI TTA'TAA
Cuts at: 6268 6268
Size: 8698
PstI C_TGCA'G
Cuts at: 830 2075 4666 6050 830
Size: 1245 2591 1384 3478
Fragments arranged by size:
3478 2591 1384 1245
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8698
PvuII CAG'CTG
Cuts at: 2064 2064
Size: 8698
SacI G_AGCT'C
Cuts at: 4758 4758
Size: 8698
SalI G'TCGA_C
Cuts at: 1936 6040 1936
Size: 4104 4594
SbfI CC_TGCA'GG
Cuts at: 6050 6050
Size: 8698
ScaI AGT'ACT
Cuts at: 3491 4046 4226 6946 8658 3491
Size: 555 180 2720 1712 3531
Fragments arranged by size:
3531 2720 1712 555 180
SfcI C'TryA_G
Cuts at: 826 1045 2071 2353 4662 4691 4759 6046 7181
Size: 219 1026 282 2309 29 68 1287 1135
Cuts at: 7181 7862 8053 826
Size: 681 191 1471
Fragments arranged by size:
2309 1471 1287 1135 1026 681 282 219
191 68 29
SfoI GGC'GCC
Cuts at: 5184 5184
Size: 8698
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8698
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8698
SmaI CCC'GGG
Cuts at: 1872 6025 1872
Size: 4153 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3526 4822 5381 5573 5584 6802
Size: 197 926 1583 1296 559 192 11 1218
Cuts at: 6802 7703 7965 820
Size: 901 262 1553
Fragments arranged by size:
1583 1553 1296 1218 926 901 559 262
197 192 11
SnaBI TAC'GTA
Cuts at: 493 6489 493
Size: 5996 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8698
SphI G_CATG'C
Cuts at: 1132 2893 3568 6056 1132
Size: 1761 675 2488 3774
Fragments arranged by size:
3774 2488 1761 675
SspI AAT'ATT
Cuts at: 5 52 5302 6622 5
Size: 47 5250 1320 2081
Fragments arranged by size:
5250 2081 1320 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8698
StyI C'CwwG_G
Cuts at: 513 1065 2305 2715 3399 3556 3817 4008 4378
Size: 552 1240 410 684 157 261 191 370
Cuts at: 4378 4450 5178 6137 513
Size: 72 728 959 3074
Fragments arranged by size:
3074 1240 959 728 684 552 410 370
261 191 157 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2320 3287 4766 5267 6725 6742 6895
Size: 1255 740 967 1479 501 1458 17 153
Cuts at: 6895 7080 7525 325
Size: 185 445 1498
Fragments arranged by size:
1498 1479 1458 1255 967 740 501 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3489 4044 4224
Size: 274 80 33 51 1443 1512 555 180
Cuts at: 4224 6944 8656 96
Size: 2720 1712 138
Fragments arranged by size:
2720 1712 1512 1443 555 274 180 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3896 3928 4949 4981 8658 8690 3896
Size: 32 1021 32 3677 32 3904
Fragments arranged by size:
3904 3677 1021 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8698
XbaI T'CTAG_A
Cuts at: 6034 6034
Size: 8698
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3229 4316 4693 5224 3229
Size: 1087 377 531 6703
Fragments arranged by size:
6703 1087 531 377
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8698
XmaI C'CCGG_G
Cuts at: 1870 6023 1870
Size: 4153 4545
XmnI GAAnn'nnTTC
Cuts at: 6827 6827
Size: 8698
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2658 6506 276
Size: 53 83 186 936 1124 3848 2468
Fragments arranged by size:
3848 2468 1124 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI BbvCI BplI Bpu10I BsiWI BsmBI BstXI
BstZ17I EcoRV FseI PacI PmlI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI