Restriction map for
pFN21AA0864
(Circular) (Six-base) MAPSORT of: pFN21AA0864.seq Check: 1834 from: 1 to: 7819
pFN21AA0864 7819 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3952 3952
Size: 7819
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5629 278
Size: 53 83 186 936 4093 2468
Fragments arranged by size:
4093 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5162 1937
Size: 3225 4594
Acc65I G'GTAC_C
Cuts at: 5140 5140
Size: 7819
AclI AA'CG_TT
Cuts at: 1563 5946 6319 1563
Size: 4383 373 3063
Fragments arranged by size:
4383 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2862 4014 5075 5882 7266 7534 1632
Size: 1230 1152 1061 807 1384 268 1917
Fragments arranged by size:
1917 1384 1230 1152 1061 807 268
AfeI AGC'GCT
Cuts at: 3788 3788
Size: 7819
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7622
AflIII A'CryG_T
Cuts at: 1203 6718 7695 1203
Size: 5515 977 1327
Fragments arranged by size:
5515 1327 977
AgeI A'CCGG_T
Cuts at: 2196 6650 2196
Size: 4454 3365
AhdI GACnn_n'nnGTC
Cuts at: 3130 6548 3130
Size: 3418 4401
AleI CACnn'nnGTG
Cuts at: 1166 2318 1166
Size: 1152 6667
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7819
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4452 4484 4452
Size: 32 7787
AlwNI CAG_nnn'CTG
Cuts at: 2854 3272 3667 3824 4432 4525 4546 4597 4750
Size: 418 395 157 608 93 21 51 153
Cuts at: 4750 5013 5562 7134 2854
Size: 263 549 1572 3539
Fragments arranged by size:
3539 1572 608 549 418 395 263 157
153 93 51 21
ApaI G_GGCC'C
Cuts at: 2979 2979
Size: 7819
ApaLI G'TGCA_C
Cuts at: 2192 2311 3449 3759 5876 7032 2192
Size: 119 1138 310 2117 1156 2979
Fragments arranged by size:
2979 2117 1156 1138 310 119
ApoI r'AATT_y
Cuts at: 1493 2036 3166 5128 5354 5506 7615 1493
Size: 543 1130 1962 226 152 2109 1697
Fragments arranged by size:
2109 1962 1697 1130 543 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6373 160
Size: 872 5341 1606
Fragments arranged by size:
5341 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7819
AvaI C'yCGr_G
Cuts at: 1870 1943 3529 4040 5144 1870
Size: 73 1586 511 1104 4545
Fragments arranged by size:
4545 1586 1104 511 73
AvrII C'CTAG_G
Cuts at: 3029 3029
Size: 7819
BamHI G'GATC_C
Cuts at: 3426 5149 5516 3426
Size: 1723 367 5729
Fragments arranged by size:
5729 1723 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2291 3964 4422 5140 6595
Size: 325 217 627 504 1673 458 718 1455
Cuts at: 6595 618
Size: 1842
Fragments arranged by size:
1842 1673 1455 718 627 504 458 325
217
BanII G_rGCy'C
Cuts at: 1391 2496 2979 3144 3717 3863 4578 4848 5138
Size: 1105 483 165 573 146 715 270 290
Cuts at: 5138 1391
Size: 4072
Fragments arranged by size:
4072 1105 715 573 483 290 270 165
146
BbeI G_GCGC'C
Cuts at: 4426 4426
Size: 7819
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2825 3981 928
Size: 971 926 1156 4766
Fragments arranged by size:
4766 1156 971 926
BbvCI CC'TCA_GC
Cuts at: 3273 4115 3273
Size: 842 6977
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2183 2217 3772 3806 6010 6044 1903
Size: 34 246 34 1555 34 2204 34 3678
Fragments arranged by size:
3678 2204 1555 246 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3345 5711 6921 3345
Size: 2366 1210 4243
Fragments arranged by size:
4243 2366 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7819
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2343 2528
Size: 107 122 71 631 605 337 334 185
Cuts at: 2528 4514 6430 136
Size: 1986 1916 1525
Fragments arranged by size:
1986 1916 1525 631 605 337 334 185
122 107 71
BglII A'GATC_T
Cuts at: 4225 7814 4225
Size: 3589 4230
BlpI GC'TnA_GC
Cuts at: 4847 5236 4847
Size: 389 7430
Bme1580I G_kGCm'C
Cuts at: 1790 2196 2294 2315 2979 3453 3763 3969 5880
Size: 406 98 21 664 474 310 206 1911
Cuts at: 5880 7036 1790
Size: 1156 2573
Fragments arranged by size:
2573 1911 1156 664 474 406 310 206
98 21
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7819
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2183 2571 3646
Size: 416 53 83 399 24 766 388 1075
Cuts at: 3646 4656 4680 4931 5550 6508 442
Size: 1010 24 251 619 958 1753
Fragments arranged by size:
1753 1075 1010 958 766 619 416 399
388 251 83 53 24 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7819
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2685 2709 3637 4048 4489 4501 4783 2685
Size: 24 928 411 441 12 282 5721
Fragments arranged by size:
5721 928 441 411 282 24 12
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2148 2450 2834 3767 4073 4106 4214 4385
Size: 909 302 384 933 306 33 108 171
Cuts at: 4385 4454 4484 4520 4547 6479 1239
Size: 69 30 36 27 1932 2579
Fragments arranged by size:
2579 1932 933 909 384 306 302 171
108 69 36 33 30 27
Bpu10I CC'TnA_GC
Cuts at: 3273 3576 3672 4115 4863 3273
Size: 303 96 443 748 6229
Fragments arranged by size:
6229 748 443 303 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4757 4886 5944 6809 7107 4757
Size: 129 1058 865 298 5469
Fragments arranged by size:
5469 1058 865 298 129
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2205 2396 2954 3515 3929 6482 882
Size: 617 706 191 558 561 414 2553 2219
Fragments arranged by size:
2553 2219 706 617 561 558 414 191
BsaAI yAC'GTr
Cuts at: 493 1204 3448 3904 4159 4173 5610 493
Size: 711 2244 456 255 14 1437 2702
Fragments arranged by size:
2702 2244 1437 711 456 255 14
BsaBI GATnn'nnATC
Cuts at: 7813 7813
Size: 7819
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2372 3078 3152 3804
Size: 53 83 186 936 839 706 74 652
Cuts at: 3804 4423 5626 6008 6643 275
Size: 619 1203 382 635 1451
Fragments arranged by size:
1451 1203 936 839 706 652 635 619
382 186 83 74 53
BsaWI w'CCGG_w
Cuts at: 2196 3855 4955 6252 6650 6924 7071 7482 2196
Size: 1659 1100 1297 398 274 147 411 2533
Fragments arranged by size:
2533 1659 1297 1100 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2012 2042 3368 3398 3998
Size: 30 165 30 609 30 1326 30 600
Cuts at: 3998 4028 4430 4460 1178
Size: 30 402 30 4537
Fragments arranged by size:
4537 1326 609 600 402 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2756 3057 3290 3601 3788 4307 4451 1186
Size: 1570 301 233 311 187 519 144 4554
Fragments arranged by size:
4554 1570 519 311 301 233 187 144
BseYI C'CCAG_C
Cuts at: 2678 2876 2882 3061 3559 7022 7518 2678
Size: 198 6 179 498 3463 496 2979
Fragments arranged by size:
3463 2979 498 496 198 179 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2050 4472 1163
Size: 573 314 2422 4510
Fragments arranged by size:
4510 2422 573 314
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2764 5269 6030 7058 1540
Size: 275 186 763 2505 761 1028 2301
Fragments arranged by size:
2505 2301 1028 763 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2196 2241 2315 2955 3453 3763 4578 4848 5880
Size: 45 74 640 498 310 815 270 1032
Cuts at: 5880 5965 7036 2196
Size: 85 1071 2979
Fragments arranged by size:
2979 1071 1032 815 640 498 310 270
85 74 45
BsmI GAATG_Cn'
Cuts at: 4510 5330 5423 4510
Size: 820 93 6906
Fragments arranged by size:
6906 820 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2196 2241 2294 2315 2496 2955 2979
Size: 399 406 45 53 21 181 459 24
Cuts at: 2979 3144 3453 3717 3763 3863 3969 4578 4848
Size: 165 309 264 46 100 106 609 270
Cuts at: 4848 5138 5880 5965 7036 1391
Size: 290 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 609 459 406 399 309
290 270 264 181 165 106 100 85
53 46 45 24 21
BspEI T'CCGG_A
Cuts at: 4955 7482 4955
Size: 2527 5292
BspHI T'CATG_A
Cuts at: 3438 5708 7438 3438
Size: 2270 1730 3819
Fragments arranged by size:
3819 2270 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3168 3533 3952 4875 5174 844
Size: 2324 365 419 923 299 3489
Fragments arranged by size:
3489 2324 923 419 365 299
BsrBI CCG'CTC
Cuts at: 1394 1823 2799 2866 3604 3621 3679 3751 4357
Size: 429 976 67 738 17 58 72 606
Cuts at: 4357 4537 5706 1394
Size: 180 1169 3507
Fragments arranged by size:
3507 1169 976 738 606 429 180 72
67 58 17
BsrDI GCAATG_nn'
Cuts at: 65 1248 4200 6314 6488 65
Size: 1183 2952 2114 174 1396
Fragments arranged by size:
2952 2114 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2196 4008 4095 6463 6650 1700
Size: 110 386 1812 87 2368 187 2869
Fragments arranged by size:
2869 2368 1812 386 187 110 87
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7819
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7819
BssSI C'ACGA_G
Cuts at: 3710 4022 5879 6891 3710
Size: 312 1857 1012 4638
Fragments arranged by size:
4638 1857 1012 312
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4450 4546 794
Size: 3656 96 4067
Fragments arranged by size:
4067 3656 96
BstBI TT'CG_AA
Cuts at: 6623 6623
Size: 7819
BstEII G'GTnAC_C
Cuts at: 1184 4004 4674 1184
Size: 2820 670 4329
Fragments arranged by size:
4329 2820 670
BstXI CCAn_nnnn'nTGG
Cuts at: 2648 2971 3121 2648
Size: 323 150 7346
Fragments arranged by size:
7346 323 150
BstYI r'GATC_y
Cuts at: 1972 3426 4225 5009 5149 5516 5901 5918 7370
Size: 1454 799 784 140 367 385 17 1452
Cuts at: 7370 7456 7468 7814 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1454 1452 799 784 385 367 346
140 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2286 3726 2286
Size: 1440 6379
BtgI C'CryG_G
Cuts at: 513 1065 3135 4947 513
Size: 552 2070 1812 3385
Fragments arranged by size:
3385 2070 1812 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3955 4837 487
Size: 683 56 2729 882 3469
Fragments arranged by size:
3469 2729 882 683 56
BtsI GCAGTG_nn'
Cuts at: 2976 3459 4559 4793 5335 6129 6149 2976
Size: 483 1100 234 542 794 20 4646
Fragments arranged by size:
4646 1100 794 542 483 234 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7819
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7784
DraI TTT'AAA
Cuts at: 5123 5123
Size: 7819
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6826 809
Size: 507 5510 1802
Fragments arranged by size:
5510 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2776 3205 3396
Size: 54 1425 50 275 18 946 429 191
Cuts at: 3396 4302 5266 6155 7784 8
Size: 906 964 889 1629 43
Fragments arranged by size:
1629 1425 964 946 906 889 429 275
191 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5266 1537
Size: 275 3454 4090
Fragments arranged by size:
4090 3454 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2733 3011 3103 3578 3948 4263 4364 5749
Size: 1380 278 92 475 370 315 101 1385
Cuts at: 5749 1353
Size: 3423
Fragments arranged by size:
3423 1385 1380 475 370 315 278 101
92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2414 3433 3746 6406 6780 6926
Size: 1064 138 825 1019 313 2660 374 146
Cuts at: 6926 387
Size: 1280
Fragments arranged by size:
2660 1280 1064 1019 825 374 313 146
138
EcoICRI GAG'CTC
Cuts at: 4576 4846 4576
Size: 270 7549
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2148 2450 2834 2862 3767 4014 4073
Size: 393 516 302 384 28 905 247 59
Cuts at: 4073 4106 4214 4385 4454 4484 4520 4547 5075
Size: 33 108 171 69 30 36 27 528
Cuts at: 5075 5882 6479 7266 7534 1239
Size: 807 597 787 268 1524
Fragments arranged by size:
1524 905 807 787 597 528 516 393
384 302 268 247 171 108 69 59
36 33 30 28 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2123 2285 3529 3671 4289 4685 4754 2123
Size: 162 1244 142 618 396 69 5188
Fragments arranged by size:
5188 1244 618 396 162 142 69
EcoO109I rG'GnC_Cy
Cuts at: 2116 2904 2975 2976 3664 3695 4414 4468 4543
Size: 788 71 1 688 31 719 54 75
Cuts at: 4543 2116
Size: 5392
Fragments arranged by size:
5392 788 719 688 75 71 54 31
1
EcoRI G'AATT_C
Cuts at: 5128 5506 5128
Size: 378 7441
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3058 3058
Size: 7819
FspI TGC'GCA
Cuts at: 3842 6325 3842
Size: 2483 5336
HaeII r_GCGC'y
Cuts at: 1678 2751 3781 3790 4146 4426 4526 6966 1678
Size: 1073 1030 9 356 280 100 2440 2531
Fragments arranged by size:
2531 2440 1073 1030 356 280 100 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2053 2085 2269 2301 3116
Size: 32 135 32 481 32 184 32 815
Cuts at: 3116 3148 3928 3960 3998 4030 4210 4242 4430
Size: 32 780 32 38 32 180 32 188
Cuts at: 4430 4462 5001 5033 6460 6492 6534 6566 1373
Size: 32 539 32 1427 32 42 32 2626
Fragments arranged by size:
2626 1427 815 780 539 481 188 184
180 135 42 38 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4399 5163 5409 669
Size: 988 281 2461 764 246 3079
Fragments arranged by size:
3079 2461 988 764 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7819
HpaI GTT'AAC
Cuts at: 5409 5409
Size: 7819
KasI G'GCGC_C
Cuts at: 4422 4422
Size: 7819
KpnI G_GTAC'C
Cuts at: 5144 5144
Size: 7819
MfeI C'AATT_G
Cuts at: 5418 5418
Size: 7819
MluI A'CGCG_T
Cuts at: 7695 7695
Size: 7819
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6933 7117 1305
Size: 5628 184 2007
Fragments arranged by size:
5628 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5997
Fragments arranged by size:
5997 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2300 2318 3848 5777 6136 6295 518
Size: 648 1134 18 1530 1929 359 159 2042
Fragments arranged by size:
2042 1929 1530 1134 648 359 159 18
MspA1I CmG'CkG
Cuts at: 1606 2854 3510 3919 4114 4246 4301 4378 4456
Size: 1248 656 409 195 132 55 77 78
Cuts at: 4456 5235 5912 7060 7305 1606
Size: 779 677 1148 245 2120
Fragments arranged by size:
2120 1248 1148 779 677 656 409 245
195 132 78 77 55
NarI GG'CG_CC
Cuts at: 4423 4423
Size: 7819
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7267
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7819
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7819
NotI GC'GGCC_GC
Cuts at: 5266 5266
Size: 7819
NruI TCG'CGA
Cuts at: 4856 4856
Size: 7819
NspI r_CATG'y
Cuts at: 1132 1229 3318 5177 6722 1132
Size: 97 2089 1859 1545 2229
Fragments arranged by size:
2229 2089 1859 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3863 4126 1348
Size: 2515 263 5041
Fragments arranged by size:
5041 2515 263
PciI A'CATG_T
Cuts at: 6718 6718
Size: 7819
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2613 4432 4525 1108
Size: 1505 1819 93 4402
Fragments arranged by size:
4402 1819 1505 93
PfoI T'CCnGG_A
Cuts at: 3948 3948
Size: 7819
PmeI GTTT'AAAC
Cuts at: 5123 5123
Size: 7819
PmlI CAC'GTG
Cuts at: 3448 3904 4173 3448
Size: 456 269 7094
Fragments arranged by size:
7094 456 269
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5888 5920 7401 7433 5888
Size: 32 1481 32 6274
Fragments arranged by size:
6274 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2116 2904 4468 2116
Size: 788 1564 5467
Fragments arranged by size:
5467 1564 788
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7819
PsiI TTA'TAA
Cuts at: 5389 5389
Size: 7819
PspOMI G'GGCC_C
Cuts at: 2975 2975
Size: 7819
PstI C_TGCA'G
Cuts at: 830 4107 4167 4446 4618 4788 5171 830
Size: 3277 60 279 172 170 383 3478
Fragments arranged by size:
3478 3277 383 279 172 170 60
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7819
PvuII CAG'CTG
Cuts at: 2854 3510 4114 2854
Size: 656 604 6559
Fragments arranged by size:
6559 656 604
SacI G_AGCT'C
Cuts at: 4578 4848 4578
Size: 270 7549
SalI G'TCGA_C
Cuts at: 1936 5161 1936
Size: 3225 4594
SapI GCTCTTCn'nnn_
Cuts at: 3103 4364 3103
Size: 1261 6558
SbfI CC_TGCA'GG
Cuts at: 4107 5171 4107
Size: 1064 6755
ScaI AGT'ACT
Cuts at: 4484 6067 7779 4484
Size: 1583 1712 4524
Fragments arranged by size:
4524 1712 1583
SfcI C'TryA_G
Cuts at: 826 1045 3256 4103 4163 4442 4614 4784 5167
Size: 219 2211 847 60 279 172 170 383
Cuts at: 5167 6302 6983 7174 826
Size: 1135 681 191 1471
Fragments arranged by size:
2211 1471 1135 847 681 383 279 219
191 172 170 60
SfiI GGCCn_nnn'nGGCC
Cuts at: 2343 2343
Size: 7819
SfoI GGC'GCC
Cuts at: 4424 4424
Size: 7819
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7819
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7819
SmaI CCC'GGG
Cuts at: 1872 5146 1872
Size: 3274 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4040 4772 4901 5923 6824 7086
Size: 197 926 2097 732 129 1022 901 262
Cuts at: 7086 820
Size: 1553
Fragments arranged by size:
2097 1553 1022 926 901 732 262 197
129
SnaBI TAC'GTA
Cuts at: 493 5610 493
Size: 5117 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7819
SphI G_CATG'C
Cuts at: 1132 5177 1132
Size: 4045 3774
SspI AAT'ATT
Cuts at: 5 52 5743 5
Size: 47 5691 2081
Fragments arranged by size:
5691 2081 47
StuI AGG'CCT
Cuts at: 1517 2930 3535 4073 5064 1517
Size: 1413 605 538 991 4272
Fragments arranged by size:
4272 1413 991 605 538
StyI C'CwwG_G
Cuts at: 513 1065 2016 2406 2523 2634 2931 3029 5258
Size: 552 951 390 117 111 297 98 2229
Cuts at: 5258 513
Size: 3074
Fragments arranged by size:
3074 2229 951 552 390 297 117 111
98
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2189 2412 3722 5846 5863 6016 6201
Size: 1255 609 223 1310 2124 17 153 185
Cuts at: 6201 6646 325
Size: 445 1498
Fragments arranged by size:
2124 1498 1310 1255 609 445 223 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4482 4824 6065
Size: 274 80 33 51 1443 2505 342 1241
Cuts at: 6065 7777 96
Size: 1712 138
Fragments arranged by size:
2505 1712 1443 1241 342 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2012 2044 2266 2298 2888 2920 3194 3226 3319
Size: 32 222 32 590 32 274 32 93
Cuts at: 3319 3351 7779 7811 2012
Size: 32 4428 32 2020
Fragments arranged by size:
4428 2020 590 274 222 93 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7819
XbaI T'CTAG_A
Cuts at: 5155 5155
Size: 7819
XhoI C'TCGA_G
Cuts at: 1943 4040 1943
Size: 2097 5722
XmaI C'CCGG_G
Cuts at: 1870 5144 1870
Size: 3274 4545
XmnI GAAnn'nnTTC
Cuts at: 2078 5948 2078
Size: 3870 3949
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5627 276
Size: 53 83 186 936 4093 2468
Fragments arranged by size:
4093 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NruI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV
FseI FspAI NaeI NgoMIV NsiI PacI PspXI PsrI
RsrII SacII SanDI SexAI SrfI SwaI XcmI