Restriction map for
pFN21AA0853
(Circular) (Six-base) MAPSORT of: pFN21AA0853.seq Check: 8188 from: 1 to: 9709
pFN21AA0853 9709 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6416 6416
Size: 9709
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7519 278
Size: 53 83 186 936 5983 2468
Fragments arranged by size:
5983 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2951 3999 6879 7052 1937
Size: 1014 1048 2880 173 4594
Fragments arranged by size:
4594 2880 1048 1014 173
Acc65I G'GTAC_C
Cuts at: 7030 7030
Size: 9709
AclI AA'CG_TT
Cuts at: 1563 7836 8209 1563
Size: 6273 373 3063
Fragments arranged by size:
6273 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2162 2673 4854 4983 5781 6778 6978 7772
Size: 530 511 2181 129 798 997 200 794
Cuts at: 7772 9156 9424 1632
Size: 1384 268 1917
Fragments arranged by size:
2181 1917 1384 997 798 794 530 511
268 200 129
AfeI AGC'GCT
Cuts at: 4478 4478
Size: 9709
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9512
AflIII A'CryG_T
Cuts at: 1203 2171 6647 8608 9585 1203
Size: 968 4476 1961 977 1327
Fragments arranged by size:
4476 1961 1327 977 968
AgeI A'CCGG_T
Cuts at: 8540 8540
Size: 9709
AhdI GACnn_n'nnGTC
Cuts at: 8438 8438
Size: 9709
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9709
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9709
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4690 4722 4690
Size: 32 9677
AlwNI CAG_nnn'CTG
Cuts at: 5202 6620 6997 7452 9024 5202
Size: 1418 377 455 1572 5887
Fragments arranged by size:
5887 1572 1418 455 377
ApaLI G'TGCA_C
Cuts at: 4803 7766 8922 4803
Size: 2963 1156 5590
Fragments arranged by size:
5590 2963 1156
ApoI r'AATT_y
Cuts at: 1493 2752 4887 6417 6563 7018 7244 7396 9505
Size: 1259 2135 1530 146 455 226 152 2109
Cuts at: 9505 1493
Size: 1697
Fragments arranged by size:
2135 2109 1697 1530 1259 455 226 152
146
AseI AT'TA_AT
Cuts at: 160 1032 2472 2534 8263 160
Size: 872 1440 62 5729 1606
Fragments arranged by size:
5729 1606 1440 872 62
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9709
AvaI C'yCGr_G
Cuts at: 1870 1943 3360 5579 6222 7034 1870
Size: 73 1417 2219 643 812 4545
Fragments arranged by size:
4545 2219 1417 812 643 73
BamHI G'GATC_C
Cuts at: 7039 7406 7039
Size: 367 9342
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 6842 7030 8485 618
Size: 325 217 627 5055 188 1455 1842
Fragments arranged by size:
5055 1842 1455 627 325 217 188
BanII G_rGCy'C
Cuts at: 1391 2304 3329 4861 6712 7028 1391
Size: 913 1025 1532 1851 316 4072
Fragments arranged by size:
4072 1851 1532 1025 913 316
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2077 2150 2385 2679 2717 3883 4383
Size: 971 178 73 235 294 38 1166 500
Cuts at: 4383 4659 5472 5612 6746 6896 6929 928
Size: 276 813 140 1134 150 33 3708
Fragments arranged by size:
3708 1166 1134 971 813 500 294 276
235 178 150 140 73 38 33
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5560 5594 7900 7934 1903
Size: 34 3623 34 2306 34 3678
Fragments arranged by size:
3678 3623 2306 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3556 6377 6995 7601 8811 3556
Size: 2821 618 606 1210 4454
Fragments arranged by size:
4454 2821 1210 618 606
BclI T'GATC_A
Cuts at: 1548 3208 5758 1548
Size: 1660 2550 5499
Fragments arranged by size:
5499 2550 1660
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8320 136
Size: 107 122 71 631 605 6648 1525
Fragments arranged by size:
6648 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5870 6262 9704 5870
Size: 392 3442 5875
Fragments arranged by size:
5875 3442 392
BlpI GC'TnA_GC
Cuts at: 7126 7126
Size: 9709
Bme1580I G_kGCm'C
Cuts at: 1790 4807 6845 7770 8926 1790
Size: 3017 2038 925 1156 2573
Fragments arranged by size:
3017 2573 2038 1156 925
BmgBI CAC'GTC
Cuts at: 1318 2290 1318
Size: 972 8737
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2070 5775 7440
Size: 416 53 83 399 24 653 3705 1665
Cuts at: 7440 8398 442
Size: 958 1753
Fragments arranged by size:
3705 1753 1665 958 653 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2697 1056
Size: 1641 8068
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2347 2347
Size: 9709
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2391 3406 5826 6396 6594 6924 8369 1239
Size: 1152 1015 2420 570 198 330 1445 2579
Fragments arranged by size:
2579 2420 1445 1152 1015 570 330 198
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3277 3424 3721 3986 4599 4615 4718 4777 5683
Size: 147 297 265 613 16 103 59 906
Cuts at: 5683 7834 8699 8997 3277
Size: 2151 865 298 3989
Fragments arranged by size:
3989 2151 906 865 613 298 297 265
147 103 59 16
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3330 3885 4185 4191 8372 882
Size: 617 1831 555 300 6 4181 2219
Fragments arranged by size:
4181 2219 1831 617 555 300 6
BsaAI yAC'GTr
Cuts at: 493 1204 2789 5521 7500 493
Size: 711 1585 2732 1979 2702
Fragments arranged by size:
2732 2702 1979 1585 711
BsaBI GATnn'nnATC
Cuts at: 3549 3567 3825 4437 6261 9703 3549
Size: 18 258 612 1824 3442 3555
Fragments arranged by size:
3555 3442 1824 612 258 18
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3241 5513 7516 7898
Size: 53 83 186 936 1708 2272 2003 382
Cuts at: 7898 8533 275
Size: 635 1451
Fragments arranged by size:
2272 2003 1708 1451 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4540 8142 8540 8814 8961 9372 4540
Size: 3602 398 274 147 411 4877
Fragments arranged by size:
4877 3602 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3079 3109 5272 5302 5584
Size: 30 165 30 1676 30 2163 30 282
Cuts at: 5584 5614 6853 6883 1178
Size: 30 1239 30 4004
Fragments arranged by size:
4004 2163 1676 1239 282 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2336 3039 4691 5280 5301 5581 5962 6476
Size: 1150 703 1652 589 21 280 381 514
Cuts at: 6476 1186
Size: 4419
Fragments arranged by size:
4419 1652 1150 703 589 514 381 280
21
BseYI C'CCAG_C
Cuts at: 2099 2959 3789 4499 5070 8912 9408 2099
Size: 860 830 710 571 3842 496 2400
Fragments arranged by size:
3842 2400 860 830 710 571 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5672 6296 6537 1163
Size: 573 3936 624 241 4335
Fragments arranged by size:
4335 3936 624 573 241
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4207 7159 7920 8948 1540
Size: 275 186 2206 2952 761 1028 2301
Fragments arranged by size:
2952 2301 2206 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3329 4807 4861 6871 7770 7855 8926 3329
Size: 1478 54 2010 899 85 1071 4112
Fragments arranged by size:
4112 2010 1478 1071 899 85 54
BsiWI C'GTAC_G
Cuts at: 3623 3623
Size: 9709
BsmI GAATG_Cn'
Cuts at: 4803 5192 7220 7313 4803
Size: 389 2028 93 7199
Fragments arranged by size:
7199 2028 389 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3150 5504 3150
Size: 2354 7355
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2304 3329 4807 4861 6712 6845 6871
Size: 399 514 1025 1478 54 1851 133 26
Cuts at: 6871 7028 7770 7855 8926 1391
Size: 157 742 85 1071 2174
Fragments arranged by size:
2174 1851 1478 1071 1025 742 514 399
157 133 85 54 26
BspEI T'CCGG_A
Cuts at: 4540 9372 4540
Size: 4832 4877
BspHI T'CATG_A
Cuts at: 2488 3211 3232 3781 4861 7598 9328 2488
Size: 723 21 549 1080 2737 1730 2869
Fragments arranged by size:
2869 2737 1730 1080 723 549 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 6416 6605 7064 844
Size: 5572 189 459 3489
Fragments arranged by size:
5572 3489 459 189
BsrBI CCG'CTC
Cuts at: 1394 1823 4069 4213 7596 1394
Size: 429 2246 144 3383 3507
Fragments arranged by size:
3507 3383 2246 429 144
BsrDI GCAATG_nn'
Cuts at: 65 1248 5149 8204 8378 65
Size: 1183 3901 3055 174 1396
Fragments arranged by size:
3901 3055 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4524 8353 8540 1700
Size: 110 2714 3829 187 2869
Fragments arranged by size:
3829 2869 2714 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9709
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9709
BssSI C'ACGA_G
Cuts at: 2199 3507 5493 6537 6862 7769 8781 2199
Size: 1308 1986 1044 325 907 1012 3127
Fragments arranged by size:
3127 1986 1308 1044 1012 907 325
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5360 6431 794
Size: 4566 1071 4072
Fragments arranged by size:
4566 4072 1071
BstBI TT'CG_AA
Cuts at: 6814 8513 6814
Size: 1699 8010
BstEII G'GTnAC_C
Cuts at: 1184 2232 1184
Size: 1048 8661
BstXI CCAn_nnnn'nTGG
Cuts at: 5654 5654
Size: 9709
BstYI r'GATC_y
Cuts at: 1972 2254 4422 5870 6205 6262 6269 6475 7039
Size: 282 2168 1448 335 57 7 206 564
Cuts at: 7039 7406 7791 7808 9260 9346 9358 9704 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
2168 1977 1452 1448 564 385 367 346
335 282 206 86 57 17 12 7
BtgI C'CryG_G
Cuts at: 513 1065 5096 513
Size: 552 4031 5126
Fragments arranged by size:
5126 4031 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3161 5528 487
Size: 683 56 1935 2367 4668
Fragments arranged by size:
4668 2367 1935 683 56
BtsI GCAGTG_nn'
Cuts at: 5334 7225 8019 8039 5334
Size: 1891 794 20 7004
Fragments arranged by size:
7004 1891 794 20
ClaI AT'CG_AT
Cuts at: 1554 5914 1554
Size: 4360 5349
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9674
DraI TTT'AAA
Cuts at: 7013 7013
Size: 9709
DraIII CAC_nnn'GTG
Cuts at: 2201 2201
Size: 9709
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8716 809
Size: 507 7400 1802
Fragments arranged by size:
7400 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3592 5996 7156
Size: 54 1425 50 275 18 1762 2404 1160
Cuts at: 7156 8045 9674 8
Size: 889 1629 43
Fragments arranged by size:
2404 1762 1629 1425 1160 889 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7156 1537
Size: 275 5344 4090
Fragments arranged by size:
5344 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2558 3334 3976 3988 4260 4413 5307 7639
Size: 1205 776 642 12 272 153 894 2332
Cuts at: 7639 1353
Size: 3423
Fragments arranged by size:
3423 2332 1205 894 776 642 272 153
12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8296 8670 8816 387
Size: 1064 138 6707 374 146 1280
Fragments arranged by size:
6707 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3327 4859 3327
Size: 1532 8177
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2162 2391 2673 3406 4854 4983 5781
Size: 393 530 229 282 733 1448 129 798
Cuts at: 5781 5826 6396 6594 6778 6924 6978 7772 8369
Size: 45 570 198 184 146 54 794 597
Cuts at: 8369 9156 9424 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1448 798 794 787 733 597 570
530 393 282 268 229 198 184 146
129 54 45
EcoRI G'AATT_C
Cuts at: 7018 7396 7018
Size: 378 9331
EcoRV GAT'ATC
Cuts at: 2824 3827 2824
Size: 1003 8706
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2970 3084 3879 5811 2970
Size: 114 795 1932 6868
Fragments arranged by size:
6868 1932 795 114
FspI TGC'GCA
Cuts at: 8215 8215
Size: 9709
HaeII r_GCGC'y
Cuts at: 1678 2284 4480 4525 4717 8856 1678
Size: 606 2196 45 192 4139 2531
Fragments arranged by size:
4139 2531 2196 606 192 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2048 2080 2312 2344 3079
Size: 32 135 32 476 32 232 32 735
Cuts at: 3079 3111 3193 3225 3311 3343 3469 3485 3501
Size: 32 82 32 86 32 126 16 16
Cuts at: 3501 3517 4407 4439 4837 4869 4943 4975 5732
Size: 16 890 32 398 32 74 32 757
Cuts at: 5732 5764 6254 6286 6320 6352 6460 6492 8350
Size: 32 490 32 34 32 108 32 1858
Cuts at: 8350 8382 8424 8456 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1858 890 757 735 490 476 398
232 135 126 108 86 82 74 42
34 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 16 16 16
HincII GTy'rAC
Cuts at: 669 1657 1938 7053 7299 669
Size: 988 281 5115 246 3079
Fragments arranged by size:
5115 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 6855 748
Size: 6107 3602
HpaI GTT'AAC
Cuts at: 7299 7299
Size: 9709
KpnI G_GTAC'C
Cuts at: 7034 7034
Size: 9709
MfeI C'AATT_G
Cuts at: 7308 7308
Size: 9709
MluI A'CGCG_T
Cuts at: 9585 9585
Size: 9709
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2718 4909 7012 8823 9007 1305
Size: 1413 2191 2103 1811 184 2007
Fragments arranged by size:
2191 2103 2007 1811 1413 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5998 10
Size: 54 1425 343 4166 3721
Fragments arranged by size:
4166 3721 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3210 3345 5652 7667 8026 8185 518
Size: 648 2044 135 2307 2015 359 159 2042
Fragments arranged by size:
2307 2044 2042 2015 648 359 159 135
MspA1I CmG'CkG
Cuts at: 1606 3085 3789 7125 7802 8950 9195 1606
Size: 1479 704 3336 677 1148 245 2120
Fragments arranged by size:
3336 2120 1479 1148 704 677 245
NaeI GCC'GGC
Cuts at: 4526 4526
Size: 9709
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 9157
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9709
NgoMIV G'CCGG_C
Cuts at: 4524 4524
Size: 9709
NheI G'CTAG_C
Cuts at: 1052 2693 1052
Size: 1641 8068
NotI GC'GGCC_GC
Cuts at: 7156 7156
Size: 9709
NspI r_CATG'y
Cuts at: 1132 1229 2175 3433 6651 7067 8612 1132
Size: 97 946 1258 3218 416 1545 2229
Fragments arranged by size:
3218 2229 1545 1258 946 416 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9709
PciI A'CATG_T
Cuts at: 2171 6647 8608 2171
Size: 4476 1961 3272
Fragments arranged by size:
4476 3272 1961
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2290 4448 1108
Size: 1182 2158 6369
Fragments arranged by size:
6369 2158 1182
PfoI T'CCnGG_A
Cuts at: 3581 4782 6940 3581
Size: 1201 2158 6350
Fragments arranged by size:
6350 2158 1201
PmeI GTTT'AAAC
Cuts at: 7013 7013
Size: 9709
PmlI CAC'GTG
Cuts at: 5521 5521
Size: 9709
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4716 4748 7778 7810 9291 9323 4716
Size: 32 3030 32 1481 32 5102
Fragments arranged by size:
5102 3030 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9709
PsiI TTA'TAA
Cuts at: 7279 7279
Size: 9709
PstI C_TGCA'G
Cuts at: 830 2722 7061 830
Size: 1892 4339 3478
Fragments arranged by size:
4339 3478 1892
PvuI CG_AT'CG
Cuts at: 2001 4207 2001
Size: 2206 7503
PvuII CAG'CTG
Cuts at: 3789 3789
Size: 9709
SacI G_AGCT'C
Cuts at: 3329 4861 3329
Size: 1532 8177
SalI G'TCGA_C
Cuts at: 1936 7051 1936
Size: 5115 4594
SapI GCTCTTCn'nnn_
Cuts at: 3334 3334
Size: 9709
SbfI CC_TGCA'GG
Cuts at: 7061 7061
Size: 9709
ScaI AGT'ACT
Cuts at: 5685 5791 7957 9669 5685
Size: 106 2166 1712 5725
Fragments arranged by size:
5725 2166 1712 106
SfcI C'TryA_G
Cuts at: 826 1045 2247 2718 3523 4480 4752 5526 7057
Size: 219 1202 471 805 957 272 774 1531
Cuts at: 7057 8192 8873 9064 826
Size: 1135 681 191 1471
Fragments arranged by size:
1531 1471 1202 1135 957 805 774 681
471 272 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9709
SgrAI Cr'CCGG_yG
Cuts at: 1700 4524 1700
Size: 2824 6885
SmaI CCC'GGG
Cuts at: 1872 7036 1872
Size: 5164 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3292 3360 3439 3736 3965 4594
Size: 197 926 1349 68 79 297 229 629
Cuts at: 4594 4614 4733 4792 5698 7813 8714 8976 820
Size: 20 119 59 906 2115 901 262 1553
Fragments arranged by size:
2115 1553 1349 926 906 901 629 297
262 229 197 119 79 68 59 20
SnaBI TAC'GTA
Cuts at: 493 7500 493
Size: 7007 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9709
SphI G_CATG'C
Cuts at: 1132 7067 1132
Size: 5935 3774
SspI AAT'ATT
Cuts at: 5 52 7633 5
Size: 47 7581 2081
Fragments arranged by size:
7581 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9709
StyI C'CwwG_G
Cuts at: 513 1065 2945 6237 7148 513
Size: 552 1880 3292 911 3074
Fragments arranged by size:
3292 3074 1880 911 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3417 3489 3693 4209 5991 7736 7753
Size: 1255 1837 72 204 516 1782 1745 17
Cuts at: 7753 7906 8091 8536 325
Size: 153 185 445 1498
Fragments arranged by size:
1837 1782 1745 1498 1255 516 445 204
185 153 72 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2111 5683 5789
Size: 274 80 33 51 1443 134 3572 106
Cuts at: 5789 7955 9667 96
Size: 2166 1712 138
Fragments arranged by size:
3572 2166 1712 1443 274 138 134 106
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2990 3022 6853 6885 9669 9701 2990
Size: 32 3831 32 2784 32 2998
Fragments arranged by size:
3831 2998 2784 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9709
XbaI T'CTAG_A
Cuts at: 2990 3332 5161 6208 7045 2990
Size: 342 1829 1047 837 5654
Fragments arranged by size:
5654 1829 1047 837 342
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5870 5870
Size: 9709
XhoI C'TCGA_G
Cuts at: 1943 3360 1943
Size: 1417 8292
XmaI C'CCGG_G
Cuts at: 1870 7034 1870
Size: 5164 4545
XmnI GAAnn'nnTTC
Cuts at: 3067 5791 5836 6891 7838 3067
Size: 2724 45 1055 947 4938
Fragments arranged by size:
4938 2724 1055 947 45
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7517 276
Size: 53 83 186 936 5983 2468
Fragments arranged by size:
5983 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BstZ17I Bsu36I EcoNI EcoO109I FseI FspAI KasI NarI
NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI