Restriction map for
pFN21AA0850
(Circular) (Six-base) MAPSORT of: pFN21AA0850.seq Check: 8781 from: 1 to: 6631
pFN21AA0850 6631 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3113 3113
Size: 6631
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4441 278
Size: 53 83 186 936 2905 2468
Fragments arranged by size:
2905 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3974 1937
Size: 2037 4594
Acc65I G'GTAC_C
Cuts at: 3952 3952
Size: 6631
AclI AA'CG_TT
Cuts at: 1563 4758 5131 1563
Size: 3195 373 3063
Fragments arranged by size:
3195 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2096 2360 2520 2868 4694 6078 6346 1632
Size: 464 264 160 348 1826 1384 268 1917
Fragments arranged by size:
1917 1826 1384 464 348 268 264 160
AflII C'TTAA_G
Cuts at: 820 1017 2516 820
Size: 197 1499 4935
Fragments arranged by size:
4935 1499 197
AflIII A'CryG_T
Cuts at: 1203 5530 6507 1203
Size: 4327 977 1327
Fragments arranged by size:
4327 1327 977
AgeI A'CCGG_T
Cuts at: 5462 5462
Size: 6631
AhdI GACnn_n'nnGTC
Cuts at: 2114 5360 2114
Size: 3246 3385
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6631
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6631
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3654 3686 3654
Size: 32 6599
AlwNI CAG_nnn'CTG
Cuts at: 2249 4374 5946 2249
Size: 2125 1572 2934
Fragments arranged by size:
2934 2125 1572
ApaLI G'TGCA_C
Cuts at: 4688 5844 4688
Size: 1156 5475
ApoI r'AATT_y
Cuts at: 1493 2041 2211 2225 2419 2493 3857 3940 4166
Size: 548 170 14 194 74 1364 83 226
Cuts at: 4166 4318 6427 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1364 548 226 194 170 152
83 74 14
AseI AT'TA_AT
Cuts at: 160 1032 5185 160
Size: 872 4153 1606
Fragments arranged by size:
4153 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6631
AvaI C'yCGr_G
Cuts at: 1870 1943 3956 1870
Size: 73 2013 4545
Fragments arranged by size:
4545 2013 73
BamHI G'GATC_C
Cuts at: 3961 4328 3961
Size: 367 6264
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3952 5407 618
Size: 325 217 627 2165 1455 1842
Fragments arranged by size:
2165 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2981 3223 3908 3950 1391
Size: 1590 242 685 42 4072
Fragments arranged by size:
4072 1590 685 242 42
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2046 3140 928
Size: 971 147 1094 4419
Fragments arranged by size:
4419 1094 971 147
BbvCI CC'TCA_GC
Cuts at: 2727 2727
Size: 6631
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4822 4856 1903
Size: 34 2885 34 3678
Fragments arranged by size:
3678 2885 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4523 5733 4523
Size: 1210 5421
BclI T'GATC_A
Cuts at: 1548 2686 1548
Size: 1138 5493
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5242 136
Size: 107 122 71 631 605 3570 1525
Fragments arranged by size:
3570 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6626 6626
Size: 6631
BlpI GC'TnA_GC
Cuts at: 4048 4048
Size: 6631
Bme1580I G_kGCm'C
Cuts at: 1790 3493 4692 5848 1790
Size: 1703 1199 1156 2573
Fragments arranged by size:
2573 1703 1199 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6631
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2614 4362 5320
Size: 416 53 83 399 24 1197 1748 958
Cuts at: 5320 442
Size: 1753
Fragments arranged by size:
1753 1748 1197 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2156 1056
Size: 1100 5531
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3681 5291 1239
Size: 2442 1610 2579
Fragments arranged by size:
2579 2442 1610
Bpu10I CC'TnA_GC
Cuts at: 2727 2859 2727
Size: 132 6499
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3908 4756 5621 5919 3908
Size: 848 865 298 4620
Fragments arranged by size:
4620 865 848 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5294 882
Size: 617 3795 2219
Fragments arranged by size:
3795 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2783 4422 493
Size: 711 1579 1639 2702
Fragments arranged by size:
2702 1639 1579 711
BsaBI GATnn'nnATC
Cuts at: 2199 2238 6625 2199
Size: 39 4387 2205
Fragments arranged by size:
4387 2205 39
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4438 4820 5455 275
Size: 53 83 186 936 2905 382 635 1451
Fragments arranged by size:
2905 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5064 5462 5736 5883 6294 5064
Size: 398 274 147 411 5401
Fragments arranged by size:
5401 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2471 2501 2977 3007 3648
Size: 30 165 30 1068 30 476 30 641
Cuts at: 3648 3654 3678 3684 1178
Size: 6 24 6 4125
Fragments arranged by size:
4125 1068 641 476 165 30 30 30
30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2519 2522 3669 1186
Size: 1333 3 1147 4148
Fragments arranged by size:
4148 1333 1147 3
BseYI C'CCAG_C
Cuts at: 2570 3800 5834 6330 2570
Size: 1230 2034 496 2871
Fragments arranged by size:
2871 2034 1230 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2630 2786 3582 1163
Size: 573 894 156 796 4212
Fragments arranged by size:
4212 894 796 573 156
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4081 4842 5870 1540
Size: 275 186 2080 761 1028 2301
Fragments arranged by size:
2301 2080 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2981 3385 4692 4777 5848 2981
Size: 404 1307 85 1071 3764
Fragments arranged by size:
3764 1307 1071 404 85
BsmI GAATG_Cn'
Cuts at: 3167 4142 4235 3167
Size: 975 93 5563
Fragments arranged by size:
5563 975 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2981 3223 3385 3493 3908 3950 4692
Size: 399 1191 242 162 108 415 42 742
Cuts at: 4692 4777 5848 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1191 1071 742 415 399 242 162
108 85 42
BspEI T'CCGG_A
Cuts at: 6294 6294
Size: 6631
BspHI T'CATG_A
Cuts at: 4520 6250 4520
Size: 1730 4901
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2350 3113 3986 844
Size: 1506 763 873 3489
Fragments arranged by size:
3489 1506 873 763
BsrBI CCG'CTC
Cuts at: 1394 1823 4518 1394
Size: 429 2695 3507
Fragments arranged by size:
3507 2695 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2581 3802 3859 5126 5300 65
Size: 1183 1333 1221 57 1267 174 1396
Fragments arranged by size:
1396 1333 1267 1221 1183 174 57
BsrFI r'CCGG_y
Cuts at: 1700 1810 5275 5462 1700
Size: 110 3465 187 2869
Fragments arranged by size:
3465 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3546 96
Size: 3450 3181
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6631
BssSI C'ACGA_G
Cuts at: 4691 5703 4691
Size: 1012 5619
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6631
BstBI TT'CG_AA
Cuts at: 3151 5435 3151
Size: 2284 4347
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6631
BstXI CCAn_nnnn'nTGG
Cuts at: 3643 3643
Size: 6631
BstYI r'GATC_y
Cuts at: 1972 3961 4328 4713 4730 6182 6268 6280 6626
Size: 1989 367 385 17 1452 86 12 346
Cuts at: 6626 1972
Size: 1977
Fragments arranged by size:
1989 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2078 2078
Size: 6631
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6079
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5892
Fragments arranged by size:
5892 683 56
BtsI GCAGTG_nn'
Cuts at: 2154 2277 2746 3840 4147 4941 4961 2154
Size: 123 469 1094 307 794 20 3824
Fragments arranged by size:
3824 1094 794 469 307 123 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6631
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6596
DraI TTT'AAA
Cuts at: 3935 3935
Size: 6631
DraIII CAC_nnn'GTG
Cuts at: 2783 2783
Size: 6631
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5638 809
Size: 507 4322 1802
Fragments arranged by size:
4322 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2089 2122 2791
Size: 54 1425 50 275 18 259 33 669
Cuts at: 2791 3277 4078 4967 6596 8
Size: 486 801 889 1629 43
Fragments arranged by size:
1629 1425 889 801 669 486 275 259
54 50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 4078 1537
Size: 275 2266 4090
Fragments arranged by size:
4090 2266 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2496 2640 2652 2836 4561 1353
Size: 1143 144 12 184 1725 3423
Fragments arranged by size:
3423 1725 1143 184 144 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5218 5592 5738 387
Size: 1064 138 3629 374 146 1280
Fragments arranged by size:
3629 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2979 2979
Size: 6631
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2360 2520 2868 3681 4694 5291
Size: 393 464 264 160 348 813 1013 597
Cuts at: 5291 6078 6346 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1013 813 787 597 464 393 348
268 264 160
EcoRI G'AATT_C
Cuts at: 3940 4318 3940
Size: 378 6253
FspI TGC'GCA
Cuts at: 5137 5137
Size: 6631
HaeII r_GCGC'y
Cuts at: 1678 5778 1678
Size: 4100 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2100 2132 3173 3205 5272
Size: 32 135 32 528 32 1041 32 2067
Cuts at: 5272 5304 5346 5378 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2067 1041 528 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3975 4221 669
Size: 988 281 2037 246 3079
Fragments arranged by size:
3079 2037 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2519 3016 748
Size: 1771 497 4363
Fragments arranged by size:
4363 1771 497
HpaI GTT'AAC
Cuts at: 4221 4221
Size: 6631
KpnI G_GTAC'C
Cuts at: 3956 3956
Size: 6631
MfeI C'AATT_G
Cuts at: 4230 4230
Size: 6631
MluI A'CGCG_T
Cuts at: 6507 6507
Size: 6631
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2517 3770 5745 5929 1305
Size: 1212 1253 1975 184 2007
Fragments arranged by size:
2007 1975 1253 1212 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2091 2124 2793 3279 10
Size: 54 1425 343 259 33 669 486 3362
Fragments arranged by size:
3362 1425 669 486 343 259 54 33
MslI CAynn'nnrTG
Cuts at: 518 1166 3285 3641 4589 4948 5107 518
Size: 648 2119 356 948 359 159 2042
Fragments arranged by size:
2119 2042 948 648 359 356 159
MspA1I CmG'CkG
Cuts at: 1606 2403 2684 4047 4724 5872 6117 1606
Size: 797 281 1363 677 1148 245 2120
Fragments arranged by size:
2120 1363 1148 797 677 281 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6079
NdeI CA'TA_TG
Cuts at: 387 3424 387
Size: 3037 3594
NheI G'CTAG_C
Cuts at: 1052 2152 1052
Size: 1100 5531
NotI GC'GGCC_GC
Cuts at: 4078 4078
Size: 6631
NspI r_CATG'y
Cuts at: 1132 1229 3989 5534 1132
Size: 97 2760 1545 2229
Fragments arranged by size:
2760 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6631
PciI A'CATG_T
Cuts at: 5530 5530
Size: 6631
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6631
PmeI GTTT'AAAC
Cuts at: 3935 3935
Size: 6631
PmlI CAC'GTG
Cuts at: 2783 2783
Size: 6631
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3654 3686 4700 4732 6213 6245 3654
Size: 32 1014 32 1481 32 4040
Fragments arranged by size:
4040 1481 1014 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3514 1613
Size: 1901 4730
PsiI TTA'TAA
Cuts at: 4201 4201
Size: 6631
PstI C_TGCA'G
Cuts at: 830 2165 2329 3124 3523 3983 830
Size: 1335 164 795 399 460 3478
Fragments arranged by size:
3478 1335 795 460 399 164
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6631
PvuII CAG'CTG
Cuts at: 2403 2684 2403
Size: 281 6350
SacI G_AGCT'C
Cuts at: 2981 2981
Size: 6631
SalI G'TCGA_C
Cuts at: 1936 3973 1936
Size: 2037 4594
SapI GCTCTTCn'nnn_
Cuts at: 2640 2640
Size: 6631
SbfI CC_TGCA'GG
Cuts at: 3983 3983
Size: 6631
ScaI AGT'ACT
Cuts at: 2992 4879 6591 2992
Size: 1887 1712 3032
Fragments arranged by size:
3032 1887 1712
SfcI C'TryA_G
Cuts at: 826 1045 2161 2325 2844 3120 3519 3813 3979
Size: 219 1116 164 519 276 399 294 166
Cuts at: 3979 5114 5795 5986 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1116 681 519 399 294 276
219 191 166 164
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6631
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6631
SmaI CCC'GGG
Cuts at: 1872 3958 1872
Size: 2086 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2516 3887 4735 5636 5898 820
Size: 197 926 573 1371 848 901 262 1553
Fragments arranged by size:
1553 1371 926 901 848 573 262 197
SnaBI TAC'GTA
Cuts at: 493 4422 493
Size: 3929 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6631
SphI G_CATG'C
Cuts at: 1132 3989 1132
Size: 2857 3774
SspI AAT'ATT
Cuts at: 5 52 4555 5
Size: 47 4503 2081
Fragments arranged by size:
4503 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6631
StyI C'CwwG_G
Cuts at: 513 1065 2525 3788 4070 513
Size: 552 1460 1263 282 3074
Fragments arranged by size:
3074 1460 1263 552 282
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2596 3649 4658 4675 4828 5013 5458
Size: 1255 1016 1053 1009 17 153 185 445
Cuts at: 5458 325
Size: 1498
Fragments arranged by size:
1498 1255 1053 1016 1009 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2673 2848 2990
Size: 274 80 33 51 1443 696 175 142
Cuts at: 2990 3238 3546 4877 6589 96
Size: 248 308 1331 1712 138
Fragments arranged by size:
1712 1443 1331 696 308 274 248 175
142 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3472 3504 3648 3680 6591 6623 3472
Size: 32 144 32 2911 32 3480
Fragments arranged by size:
3480 2911 144 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6631
XbaI T'CTAG_A
Cuts at: 2384 3967 2384
Size: 1583 5048
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3727 3832 3727
Size: 105 6526
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6631
XmaI C'CCGG_G
Cuts at: 1870 3956 1870
Size: 2086 4545
XmnI GAAnn'nnTTC
Cuts at: 2661 3147 4760 2661
Size: 486 1613 4532
Fragments arranged by size:
4532 1613 486
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4439 276
Size: 53 83 186 936 2905 2468
Fragments arranged by size:
2905 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI