Restriction map for
pFN21AA0835
(Circular) (Six-base) MAPSORT of: pFN21AA0835.seq Check: 2288 from: 1 to: 8068
pFN21AA0835 8068 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2601 2601
Size: 8068
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2363 5878 278
Size: 53 83 186 936 827 3515 2468
Fragments arranged by size:
3515 2468 936 827 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3993 5411 1937
Size: 2056 1418 4594
Fragments arranged by size:
4594 2056 1418
Acc65I G'GTAC_C
Cuts at: 5389 5389
Size: 8068
AclI AA'CG_TT
Cuts at: 1563 6195 6568 1563
Size: 4632 373 3063
Fragments arranged by size:
4632 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2261 2418 2550 2582 3185 3476 4299 4382
Size: 629 157 132 32 603 291 823 83
Cuts at: 4382 4841 5141 6131 7515 7783 1632
Size: 459 300 990 1384 268 1917
Fragments arranged by size:
1917 1384 990 823 629 603 459 300
291 268 157 132 83 32
AfeI AGC'GCT
Cuts at: 2696 2696
Size: 8068
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7871
AflIII A'CryG_T
Cuts at: 1203 4992 5292 6967 7944 1203
Size: 3789 300 1675 977 1327
Fragments arranged by size:
3789 1675 1327 977 300
AgeI A'CCGG_T
Cuts at: 6899 6899
Size: 8068
AhdI GACnn_n'nnGTC
Cuts at: 6797 6797
Size: 8068
AleI CACnn'nnGTG
Cuts at: 1166 2297 1166
Size: 1131 6937
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3815 4768 1184
Size: 2631 953 4484
Fragments arranged by size:
4484 2631 953
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4436 4468 4436
Size: 32 8036
AlwNI CAG_nnn'CTG
Cuts at: 2579 2707 2722 2930 3095 4068 5811 7383 2579
Size: 128 15 208 165 973 1743 1572 3264
Fragments arranged by size:
3264 1743 1572 973 208 165 128 15
ApaI G_GGCC'C
Cuts at: 2996 2996
Size: 8068
ApaLI G'TGCA_C
Cuts at: 2588 6125 7281 2588
Size: 3537 1156 3375
Fragments arranged by size:
3537 3375 1156
ApoI r'AATT_y
Cuts at: 1493 4309 5260 5377 5603 5755 7864 1493
Size: 2816 951 117 226 152 2109 1697
Fragments arranged by size:
2816 2109 1697 951 226 152 117
AseI AT'TA_AT
Cuts at: 160 1032 6622 160
Size: 872 5590 1606
Fragments arranged by size:
5590 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8068
AvaI C'yCGr_G
Cuts at: 1870 1943 2387 3126 3659 4667 4849 5393 1870
Size: 73 444 739 533 1008 182 544 4545
Fragments arranged by size:
4545 1008 739 544 533 444 182 73
BamHI G'GATC_C
Cuts at: 2429 3417 3627 5398 5765 2429
Size: 988 210 1771 367 4732
Fragments arranged by size:
4732 1771 988 367 210
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2145 4639 5389 6844 618
Size: 325 217 627 358 2494 750 1455 1842
Fragments arranged by size:
2494 1842 1455 750 627 358 325 217
BanII G_rGCy'C
Cuts at: 1391 2035 2411 2988 2996 4707 5387 1391
Size: 644 376 577 8 1711 680 4072
Fragments arranged by size:
4072 1711 680 644 577 376 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2028 2955 928
Size: 971 129 927 6041
Fragments arranged by size:
6041 971 927 129
BbvCI CC'TCA_GC
Cuts at: 2087 2199 3944 2087
Size: 112 1745 6211
Fragments arranged by size:
6211 1745 112
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5347 5381 6259 6293 1903
Size: 34 3410 34 878 34 3678
Fragments arranged by size:
3678 3410 878 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4338 5960 7170 4338
Size: 1622 1210 5236
Fragments arranged by size:
5236 1622 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8068
BfrBI ATG'CAT
Cuts at: 3442 3442
Size: 8068
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2975 3167 3228
Size: 107 122 71 631 605 1303 192 61
Cuts at: 3228 3396 3722 6679 136
Size: 168 326 2957 1525
Fragments arranged by size:
2957 1525 1303 631 605 326 192 168
122 107 71 61
BglII A'GATC_T
Cuts at: 3429 5097 8063 3429
Size: 1668 2966 3434
Fragments arranged by size:
3434 2966 1668
BlpI GC'TnA_GC
Cuts at: 2205 5485 2205
Size: 3280 4788
Bme1580I G_kGCm'C
Cuts at: 1790 2124 2592 2996 3464 4538 4685 6129 7285
Size: 334 468 404 468 1074 147 1444 1156
Cuts at: 7285 1790
Size: 2573
Fragments arranged by size:
2573 1444 1156 1074 468 468 404 334
147
BmgBI CAC'GTC
Cuts at: 1318 2125 4993 1318
Size: 807 2868 4393
Fragments arranged by size:
4393 2868 807
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2710 3000 4150
Size: 416 53 83 399 24 1293 290 1150
Cuts at: 4150 4679 5799 6757 442
Size: 529 1120 958 1753
Fragments arranged by size:
1753 1293 1150 1120 958 529 416 399
290 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8068
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3061 3760 5074 3061
Size: 699 1314 6055
Fragments arranged by size:
6055 1314 699
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2125 2210 2360 2744 3167 3755 5198 5351
Size: 886 85 150 384 423 588 1443 153
Cuts at: 5351 6728 1239
Size: 1377 2579
Fragments arranged by size:
2579 1443 1377 886 588 423 384 153
150 85
Bpu10I CC'TnA_GC
Cuts at: 2087 2116 2199 2982 3780 3944 5192 2087
Size: 29 83 783 798 164 1248 4963
Fragments arranged by size:
4963 1248 798 783 164 83 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4036 4514 6193 7058 7356 4036
Size: 478 1679 865 298 4748
Fragments arranged by size:
4748 1679 865 478 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2063 2528 5034 6731 882
Size: 617 564 465 2506 1697 2219
Fragments arranged by size:
2506 2219 1697 617 564 465
BsaAI yAC'GTr
Cuts at: 493 1204 3493 5859 493
Size: 711 2289 2366 2702
Fragments arranged by size:
2702 2366 2289 711
BsaBI GATnn'nnATC
Cuts at: 8062 8062
Size: 8068
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2307 2360 3870 4101
Size: 53 83 186 936 774 53 1510 231
Cuts at: 4101 5875 6257 6892 275
Size: 1774 382 635 1451
Fragments arranged by size:
1774 1510 1451 936 774 635 382 231
186 83 53 53
BsaWI w'CCGG_w
Cuts at: 4590 4896 6501 6899 7173 7320 7731 4590
Size: 306 1605 398 274 147 411 4927
Fragments arranged by size:
4927 1605 411 398 306 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3059 3089 3948 3978 4310
Size: 30 165 30 1656 30 859 30 332
Cuts at: 4310 4340 4436 4466 4620 4650 5282 5312 1178
Size: 30 96 30 154 30 632 30 3934
Fragments arranged by size:
3934 1656 859 632 332 165 154 96
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2449 2642 2684 2756 2795 2804 2807 2810
Size: 1263 193 42 72 39 9 3 3
Cuts at: 2810 2813 2816 2825 2828 2831 2834 2861 2864
Size: 3 3 9 3 3 3 27 3
Cuts at: 2864 2879 2882 2885 2900 2903 2924 2927 2930
Size: 15 3 3 15 3 21 3 3
Cuts at: 2930 2933 4244 4256 4331 5024 5092 5147 5150
Size: 3 1311 12 75 693 68 55 3
Cuts at: 5150 5168 1186
Size: 18 4086
Fragments arranged by size:
4086 1311 1263 693 193 75 72 68
55 42 39 27 21 18 15 15
12 9 9 3 3 3 3 3
3 3 3 3 3 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 3226 3924 3968 4055 4107 4133 7271 7767 3226
Size: 698 44 87 52 26 3138 496 3527
Fragments arranged by size:
3527 3138 698 496 87 52 44 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3460 3864 5102 1163
Size: 573 1724 404 1238 4129
Fragments arranged by size:
4129 1724 1238 573 404
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3625 5518 6279 7307 1540
Size: 275 186 1624 1893 761 1028 2301
Fragments arranged by size:
2301 1893 1624 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2035 2211 2592 2988 5282 6129 6214 7285 2035
Size: 176 381 396 2294 847 85 1071 2818
Fragments arranged by size:
2818 2294 1071 847 396 381 176 85
BsmI GAATG_Cn'
Cuts at: 5579 5672 5579
Size: 93 7975
BsmBI CGTCTCn'nnnn_
Cuts at: 2351 2351
Size: 8068
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2035 2124 2211 2411 2592 2988 2996
Size: 399 245 89 87 200 181 396 8
Cuts at: 2996 3464 4538 4685 4707 5282 5387 6129 6214
Size: 468 1074 147 22 575 105 742 85
Cuts at: 6214 7285 1391
Size: 1071 2174
Fragments arranged by size:
2174 1074 1071 742 575 468 399 396
245 200 181 147 105 89 87 85
22 8
BspEI T'CCGG_A
Cuts at: 7731 7731
Size: 8068
BspHI T'CATG_A
Cuts at: 5957 7687 5957
Size: 1730 6338
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2601 4847 5423 844
Size: 1757 2246 576 3489
Fragments arranged by size:
3489 2246 1757 576
BsrBI CCG'CTC
Cuts at: 1394 1823 3045 4321 5955 1394
Size: 429 1222 1276 1634 3507
Fragments arranged by size:
3507 1634 1276 1222 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3537 6563 6737 65
Size: 1183 2289 3026 174 1396
Fragments arranged by size:
3026 2289 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3684 4191 6712 6899 1700
Size: 110 1874 507 2521 187 2869
Fragments arranged by size:
2869 2521 1874 507 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8068
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8068
BssSI C'ACGA_G
Cuts at: 4611 6128 7140 4611
Size: 1517 1012 5539
Fragments arranged by size:
5539 1517 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3189 4068 4928 794
Size: 2395 879 860 3934
Fragments arranged by size:
3934 2395 879 860
BstBI TT'CG_AA
Cuts at: 6872 6872
Size: 8068
BstEII G'GTnAC_C
Cuts at: 1184 3751 1184
Size: 2567 5501
BstXI CCAn_nnnn'nTGG
Cuts at: 3733 4877 3733
Size: 1144 6924
BstYI r'GATC_y
Cuts at: 1972 2429 3295 3417 3429 3595 3627 5034 5097
Size: 457 866 122 12 166 32 1407 63
Cuts at: 5097 5398 5765 6150 6167 7619 7705 7717 8063
Size: 301 367 385 17 1452 86 12 346
Cuts at: 8063 1972
Size: 1977
Fragments arranged by size:
1977 1452 1407 866 457 385 367 346
301 166 122 86 63 32 17 12
12
Bsu36I CC'TnA_GG
Cuts at: 3632 4239 3632
Size: 607 7461
BtgI C'CryG_G
Cuts at: 513 1065 5076 5106 513
Size: 552 4011 30 3475
Fragments arranged by size:
4011 3475 552 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2366 2479 3142 487
Size: 683 56 1140 113 663 5413
Fragments arranged by size:
5413 1140 683 663 113 56
BtsI GCAGTG_nn'
Cuts at: 2290 2451 3894 5584 6378 6398 2290
Size: 161 1443 1690 794 20 3960
Fragments arranged by size:
3960 1690 1443 794 161 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8068
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8033
DraI TTT'AAA
Cuts at: 5372 5372
Size: 8068
DraIII CAC_nnn'GTG
Cuts at: 2215 2215
Size: 8068
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7075 809
Size: 507 5759 1802
Fragments arranged by size:
5759 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2172 2565 3355
Size: 54 1425 50 275 18 342 393 790
Cuts at: 3355 3723 4429 4561 4761 4867 5515 6404 8033
Size: 368 706 132 200 106 648 889 1629
Cuts at: 8033 8
Size: 43
Fragments arranged by size:
1629 1425 889 790 706 648 393 368
342 275 200 132 106 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5515 1537
Size: 275 3703 4090
Fragments arranged by size:
4090 3703 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2174 2526 2541 2651 2772 2802 2826 2832
Size: 821 352 15 110 121 30 24 6
Cuts at: 2832 2838 2850 2856 2871 2877 2889 2895 2901
Size: 6 12 6 15 6 12 6 6
Cuts at: 2901 3272 4299 5998 1353
Size: 371 1027 1699 3423
Fragments arranged by size:
3423 1699 1027 821 371 352 121 110
30 24 15 15 12 12 6 6
6 6 6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5216 6655 7029 7175 387
Size: 1064 138 3627 1439 374 146 1280
Fragments arranged by size:
3627 1439 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2033 2986 2033
Size: 953 7115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2125 2210 2261 2360 2418 2550 2582
Size: 393 493 85 51 99 58 132 32
Cuts at: 2582 2744 3167 3185 3476 3755 4299 4382 4841
Size: 162 423 18 291 279 544 83 459
Cuts at: 4841 5141 5198 5351 6131 6728 7515 7783 1239
Size: 300 57 153 780 597 787 268 1524
Fragments arranged by size:
1524 787 780 597 544 493 459 423
393 300 291 279 268 162 153 132
99 85 83 58 57 51 32 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2597 3149 4226 4735 5195 2597
Size: 552 1077 509 460 5470
Fragments arranged by size:
5470 1077 552 509 460
EcoO109I rG'GnC_Cy
Cuts at: 2050 2060 2576 2641 2704 2977 2992 3664 3961
Size: 10 516 65 63 273 15 672 297
Cuts at: 3961 4663 4785 4963 5200 5308 5323 2050
Size: 702 122 178 237 108 15 4795
Fragments arranged by size:
4795 702 672 516 297 273 237 178
122 108 65 63 15 15 10
EcoRI G'AATT_C
Cuts at: 5377 5755 5377
Size: 378 7690
EcoRV GAT'ATC
Cuts at: 3985 3985
Size: 8068
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2662 3772 2662
Size: 1110 6958
FspI TGC'GCA
Cuts at: 3182 6574 3182
Size: 3392 4676
FspAI rTGC'GCAy
Cuts at: 3182 3182
Size: 8068
HaeII r_GCGC'y
Cuts at: 1678 2199 2451 2698 4114 4131 7215 1678
Size: 521 252 247 1416 17 3084 2531
Fragments arranged by size:
3084 2531 1416 521 252 247 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3059 3091 3313 3345 3580
Size: 32 135 32 1487 32 222 32 235
Cuts at: 3580 3612 5282 5314 6709 6741 6783 6815 1373
Size: 32 1670 32 1395 32 42 32 2626
Fragments arranged by size:
2626 1670 1487 1395 235 222 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3087 5412 5658 669
Size: 988 281 1149 2325 246 3079
Fragments arranged by size:
3079 2325 1149 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8068
HpaI GTT'AAC
Cuts at: 5658 5658
Size: 8068
KpnI G_GTAC'C
Cuts at: 5393 5393
Size: 8068
MfeI C'AATT_G
Cuts at: 5667 5667
Size: 8068
MluI A'CGCG_T
Cuts at: 7944 7944
Size: 8068
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2457 3084 3356 4281 4977 5079 5325 7182
Size: 1152 627 272 925 696 102 246 1857
Cuts at: 7182 7366 1305
Size: 184 2007
Fragments arranged by size:
2007 1857 1152 925 696 627 272 246
184 102
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2174 3357 3725 4563 4869
Size: 54 1425 343 342 1183 368 838 306
Cuts at: 4869 10
Size: 3209
Fragments arranged by size:
3209 1425 1183 838 368 343 342 306
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2297 4875 5291 6026 6385 6544 518
Size: 648 1131 2578 416 735 359 159 2042
Fragments arranged by size:
2578 2042 1131 735 648 416 359 159
MspA1I CmG'CkG
Cuts at: 1606 2078 2091 2396 3226 4068 4428 4719 5023
Size: 472 13 305 830 842 360 291 304
Cuts at: 5023 5086 5484 6161 7309 7554 1606
Size: 63 398 677 1148 245 2120
Fragments arranged by size:
2120 1148 842 830 677 472 398 360
305 304 291 245 63 13
NcoI C'CATG_G
Cuts at: 513 1065 5076 5106 513
Size: 552 4011 30 3475
Fragments arranged by size:
4011 3475 552 30
NdeI CA'TA_TG
Cuts at: 387 3673 387
Size: 3286 4782
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8068
NotI GC'GGCC_GC
Cuts at: 5515 5515
Size: 8068
NsiI A_TGCA'T
Cuts at: 3444 3444
Size: 8068
NspI r_CATG'y
Cuts at: 1132 1229 3802 4024 5296 5426 6971 1132
Size: 97 2573 222 1272 130 1545 2229
Fragments arranged by size:
2573 2229 1545 1272 222 130 97
PasI CC'CwG_GG
Cuts at: 1348 3711 1348
Size: 2363 5705
PciI A'CATG_T
Cuts at: 5292 6967 5292
Size: 1675 6393
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2579 2722 1108
Size: 1471 143 6454
Fragments arranged by size:
6454 1471 143
PfoI T'CCnGG_A
Cuts at: 3077 3144 4808 5328 3077
Size: 67 1664 520 5817
Fragments arranged by size:
5817 1664 520 67
PmeI GTTT'AAAC
Cuts at: 5372 5372
Size: 8068
PmlI CAC'GTG
Cuts at: 3493 3493
Size: 8068
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4436 4468 6137 6169 7650 7682 4436
Size: 32 1669 32 1481 32 4822
Fragments arranged by size:
4822 1669 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2060 2576 2704 3664 3961 4663 4785 4963 5308
Size: 516 128 960 297 702 122 178 345
Cuts at: 5308 5323 2060
Size: 15 4805
Fragments arranged by size:
4805 960 702 516 345 297 178 128
122 15
PshAI GACnn'nnGTC
Cuts at: 1613 2709 1613
Size: 1096 6972
PsiI TTA'TAA
Cuts at: 5638 5638
Size: 8068
PspOMI G'GGCC_C
Cuts at: 2992 2992
Size: 8068
PspXI vC'TCGA_Gb
Cuts at: 3659 3659
Size: 8068
PstI C_TGCA'G
Cuts at: 830 2083 2611 4073 5091 5420 830
Size: 1253 528 1462 1018 329 3478
Fragments arranged by size:
3478 1462 1253 1018 528 329
PvuI CG_AT'CG
Cuts at: 2001 3625 2001
Size: 1624 6444
PvuII CAG'CTG
Cuts at: 2078 2091 2396 3226 4068 5023 5086 2078
Size: 13 305 830 842 955 63 5060
Fragments arranged by size:
5060 955 842 830 305 63 13
SacI G_AGCT'C
Cuts at: 2035 2988 2035
Size: 953 7115
SalI G'TCGA_C
Cuts at: 1936 5410 1936
Size: 3474 4594
SanDI GG'GwC_CC
Cuts at: 4785 4785
Size: 8068
SbfI CC_TGCA'GG
Cuts at: 5420 5420
Size: 8068
ScaI AGT'ACT
Cuts at: 2138 3023 6316 8028 2138
Size: 885 3293 1712 2178
Fragments arranged by size:
3293 2178 1712 885
SexAI A'CCwGG_T
Cuts at: 2211 2211
Size: 8068
SfcI C'TryA_G
Cuts at: 826 1045 2079 2473 2607 3286 4069 5087 5416
Size: 219 1034 394 134 679 783 1018 329
Cuts at: 5416 6551 7232 7423 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1034 1018 783 681 679 394
329 219 191 134
SfiI GGCCn_nnn'nGGCC
Cuts at: 3722 3722
Size: 8068
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8068
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8068
SmaI CCC'GGG
Cuts at: 1872 3128 4851 5395 1872
Size: 1256 1723 544 4545
Fragments arranged by size:
4545 1723 1256 544
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3659 4015 4529 6172 7073 7335
Size: 197 926 1716 356 514 1643 901 262
Cuts at: 7335 820
Size: 1553
Fragments arranged by size:
1716 1643 1553 926 901 514 356 262
197
SnaBI TAC'GTA
Cuts at: 493 5859 493
Size: 5366 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8068
SphI G_CATG'C
Cuts at: 1132 3802 4024 5426 1132
Size: 2670 222 1402 3774
Fragments arranged by size:
3774 2670 1402 222
SspI AAT'ATT
Cuts at: 5 52 5992 5
Size: 47 5940 2081
Fragments arranged by size:
5940 2081 47
StuI AGG'CCT
Cuts at: 1517 4169 1517
Size: 2652 5416
StyI C'CwwG_G
Cuts at: 513 1065 2046 2463 3726 3747 4374 4647 5076
Size: 552 981 417 1263 21 627 273 429
Cuts at: 5076 5106 5507 513
Size: 30 401 3074
Fragments arranged by size:
3074 1263 981 627 552 429 417 401
273 30 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3019 3086 3519 3724 4657 4697 4849
Size: 1255 1439 67 433 205 933 40 152
Cuts at: 4849 4907 6095 6112 6265 6450 6895 325
Size: 58 1188 17 153 185 445 1498
Fragments arranged by size:
1498 1439 1255 1188 933 445 433 205
185 153 152 67 58 40 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2136 3021 5295
Size: 274 80 33 51 1443 159 885 2274
Cuts at: 5295 6314 8026 96
Size: 1019 1712 138
Fragments arranged by size:
2274 1712 1443 1019 885 274 159 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2201 2233 3948 3980 4620 4652 8028 8060 2201
Size: 32 1715 32 640 32 3376 32 2209
Fragments arranged by size:
3376 2209 1715 640 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8068
XbaI T'CTAG_A
Cuts at: 5404 5404
Size: 8068
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3718 3976 5062 3718
Size: 258 1086 6724
Fragments arranged by size:
6724 1086 258
XhoI C'TCGA_G
Cuts at: 1943 3659 1943
Size: 1716 6352
XmaI C'CCGG_G
Cuts at: 1870 3126 4849 5393 1870
Size: 1256 1723 544 4545
Fragments arranged by size:
4545 1723 1256 544
XmnI GAAnn'nnTTC
Cuts at: 6197 6197
Size: 8068
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2361 5876 276
Size: 53 83 186 936 827 3515 2468
Fragments arranged by size:
3515 2468 936 827 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SalI SanDI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BsiWI BstZ17I FseI KasI
NaeI NarI NgoMIV NruI PacI PsrI RsrII SacII
SapI SfoI SrfI SwaI