Restriction map for
pFN21AA0829
(Circular) (Six-base) MAPSORT of: pFN21AA0829.seq Check: 8876 from: 1 to: 8395
pFN21AA0829 8395 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2416 6205 278
Size: 53 83 186 936 880 3789 2468
Fragments arranged by size:
3789 2468 936 880 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2542 4493 5396 5738 1937
Size: 605 1951 903 342 4594
Fragments arranged by size:
4594 1951 903 605 342
Acc65I G'GTAC_C
Cuts at: 5716 5716
Size: 8395
AclI AA'CG_TT
Cuts at: 1563 6522 6895 1563
Size: 4959 373 3063
Fragments arranged by size:
4959 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3272 3636 3824 6458 7842 8110 1632
Size: 1640 364 188 2634 1384 268 1917
Fragments arranged by size:
2634 1917 1640 1384 364 268 188
AflII C'TTAA_G
Cuts at: 820 1017 4555 4913 820
Size: 197 3538 358 4302
Fragments arranged by size:
4302 3538 358 197
AflIII A'CryG_T
Cuts at: 1203 3372 5459 7294 8271 1203
Size: 2169 2087 1835 977 1327
Fragments arranged by size:
2169 2087 1835 1327 977
AgeI A'CCGG_T
Cuts at: 7226 7226
Size: 8395
AhdI GACnn_n'nnGTC
Cuts at: 7124 7124
Size: 8395
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8395
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2303 1184
Size: 1119 7276
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3942 3974 3942
Size: 32 8363
AlwNI CAG_nnn'CTG
Cuts at: 6138 7710 6138
Size: 1572 6823
ApaI G_GGCC'C
Cuts at: 3409 3409
Size: 8395
ApaLI G'TGCA_C
Cuts at: 6452 7608 6452
Size: 1156 7239
ApoI r'AATT_y
Cuts at: 1493 2200 2789 3113 3478 4973 5315 5594 5704
Size: 707 589 324 365 1495 342 279 110
Cuts at: 5704 5930 6082 8191 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1495 707 589 365 342 324
279 226 152 110
AseI AT'TA_AT
Cuts at: 160 1032 5123 6949 160
Size: 872 4091 1826 1606
Fragments arranged by size:
4091 1826 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8395
AvaI C'yCGr_G
Cuts at: 1870 1943 4425 5720 1870
Size: 73 2482 1295 4545
Fragments arranged by size:
4545 2482 1295 73
BamHI G'GATC_C
Cuts at: 4199 5725 6092 4199
Size: 1526 367 6502
Fragments arranged by size:
6502 1526 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5716 7171 618
Size: 325 217 627 3929 1455 1842
Fragments arranged by size:
3929 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3409 4095 4258 5714 1391
Size: 2018 686 163 1456 4072
Fragments arranged by size:
4072 2018 1456 686 163
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3356 3532 5058 928
Size: 971 1457 176 1526 4265
Fragments arranged by size:
4265 1526 1457 971 176
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6586 6620 1903
Size: 34 4649 34 3678
Fragments arranged by size:
4649 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4310 5134 5657 6287 7497 4310
Size: 824 523 630 1210 5208
Fragments arranged by size:
5208 1210 824 630 523
BclI T'GATC_A
Cuts at: 1548 2998 3748 1548
Size: 1450 750 6195
Fragments arranged by size:
6195 1450 750
BfrBI ATG'CAT
Cuts at: 4123 4640 4123
Size: 517 7878
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7006 136
Size: 107 122 71 631 605 5334 1525
Fragments arranged by size:
5334 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2641 3703 8390 2641
Size: 1062 4687 2646
Fragments arranged by size:
4687 2646 1062
BlpI GC'TnA_GC
Cuts at: 5812 5812
Size: 8395
Bme1580I G_kGCm'C
Cuts at: 1790 3409 6456 7612 1790
Size: 1619 3047 1156 2573
Fragments arranged by size:
3047 2573 1619 1156
BmgBI CAC'GTC
Cuts at: 1318 3056 1318
Size: 1738 6657
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2545 4016 6126
Size: 416 53 83 399 24 1128 1471 2110
Cuts at: 6126 7084 442
Size: 958 1753
Fragments arranged by size:
2110 1753 1471 1128 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8395
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4386 4386
Size: 8395
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2343 3555 7055 1239
Size: 1104 1212 3500 2579
Fragments arranged by size:
3500 2579 1212 1104
Bpu10I CC'TnA_GC
Cuts at: 5622 5622
Size: 8395
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2783 3519 4471 6520 7385 7683 2783
Size: 736 952 2049 865 298 3495
Fragments arranged by size:
3495 2049 952 865 736 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3585 7058 882
Size: 617 2086 3473 2219
Fragments arranged by size:
3473 2219 2086 617
BsaAI yAC'GTr
Cuts at: 493 1204 3511 5453 6186 493
Size: 711 2307 1942 733 2702
Fragments arranged by size:
2702 2307 1942 733 711
BsaBI GATnn'nnATC
Cuts at: 2997 8389 2997
Size: 5392 3003
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2413 6202 6584 7219
Size: 53 83 186 936 880 3789 382 635
Cuts at: 7219 275
Size: 1451
Fragments arranged by size:
3789 1451 936 880 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6828 7226 7500 7647 8058 6828
Size: 398 274 147 411 7165
Fragments arranged by size:
7165 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3115 3145 3942 3972 1178
Size: 30 165 30 1712 30 797 30 5601
Fragments arranged by size:
5601 1712 797 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3522 4168 1186
Size: 2336 646 5413
Fragments arranged by size:
5413 2336 646
BseYI C'CCAG_C
Cuts at: 3911 7598 8094 3911
Size: 3687 496 4212
Fragments arranged by size:
4212 3687 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2263 3078 3120 4851 5433 1163
Size: 573 527 815 42 1731 582 4125
Fragments arranged by size:
4125 1731 815 582 573 527 42
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5845 6606 7634 1540
Size: 275 186 3844 761 1028 2301
Fragments arranged by size:
3844 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4095 4258 4367 6456 6541 7612 4095
Size: 163 109 2089 85 1071 4878
Fragments arranged by size:
4878 2089 1071 163 109 85
BsmI GAATG_Cn'
Cuts at: 5906 5999 5906
Size: 93 8302
BsmBI CGTCTCn'nnnn_
Cuts at: 3046 3133 4514 3046
Size: 87 1381 6927
Fragments arranged by size:
6927 1381 87
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3409 4095 4258 4367 5714 6456 6541
Size: 399 1619 686 163 109 1347 742 85
Cuts at: 6541 7612 1391
Size: 1071 2174
Fragments arranged by size:
2174 1619 1347 1071 742 686 399 163
109 85
BspEI T'CCGG_A
Cuts at: 8058 8058
Size: 8395
BspHI T'CATG_A
Cuts at: 6284 8014 6284
Size: 1730 6665
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5750 844
Size: 4906 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 6282 1394
Size: 429 4459 3507
Fragments arranged by size:
4459 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2803 2976 3261 4398 6890 7064 65
Size: 1183 1555 173 285 1137 2492 174 1396
Fragments arranged by size:
2492 1555 1396 1183 1137 285 174 173
BsrFI r'CCGG_y
Cuts at: 1700 1810 7039 7226 1700
Size: 110 5229 187 2869
Fragments arranged by size:
5229 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3226 5271 5462 96
Size: 3130 2045 191 3029
Fragments arranged by size:
3130 3029 2045 191
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8395
BssSI C'ACGA_G
Cuts at: 6455 7467 6455
Size: 1012 7383
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2384 2975 3103 4637 794
Size: 1590 591 128 1534 4552
Fragments arranged by size:
4552 1590 1534 591 128
BstBI TT'CG_AA
Cuts at: 7199 7199
Size: 8395
BstEII G'GTnAC_C
Cuts at: 1184 3915 1184
Size: 2731 5664
BstXI CCAn_nnnn'nTGG
Cuts at: 2038 2125 2038
Size: 87 8308
BstYI r'GATC_y
Cuts at: 1972 2641 3703 4199 5131 5725 6092 6477 6494
Size: 669 1062 496 932 594 367 385 17
Cuts at: 6494 7946 8032 8044 8390 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1062 932 669 594 496 385
367 346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2865 2865
Size: 8395
BtgI C'CryG_G
Cuts at: 513 1065 2004 4907 513
Size: 552 939 2903 4001
Fragments arranged by size:
4001 2903 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7656
Fragments arranged by size:
7656 683 56
BtsI GCAGTG_nn'
Cuts at: 2580 5911 6705 6725 2580
Size: 3331 794 20 4250
Fragments arranged by size:
4250 3331 794 20
ClaI AT'CG_AT
Cuts at: 1554 3489 1554
Size: 1935 6460
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8360
DraI TTT'AAA
Cuts at: 3154 5218 5699 3154
Size: 2064 481 5850
Fragments arranged by size:
5850 2064 481
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7402 809
Size: 507 6086 1802
Fragments arranged by size:
6086 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2602 4137 5842
Size: 54 1425 50 275 18 772 1535 1705
Cuts at: 5842 6731 8360 8
Size: 889 1629 43
Fragments arranged by size:
1705 1629 1535 1425 889 772 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5842 1537
Size: 275 4030 4090
Fragments arranged by size:
4090 4030 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3159 3324 5068 5570 6325 1353
Size: 1806 165 1744 502 755 3423
Fragments arranged by size:
3423 1806 1744 755 502 165
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6982 7356 7502 387
Size: 1064 138 5393 374 146 1280
Fragments arranged by size:
5393 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4093 4256 4093
Size: 163 8232
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2343 3272 3555 3636 3824 6458 7055
Size: 393 711 929 283 81 188 2634 597
Cuts at: 7055 7842 8110 1239
Size: 787 268 1524
Fragments arranged by size:
2634 1524 929 787 711 597 393 283
268 188 81
EcoO109I rG'GnC_Cy
Cuts at: 3405 3406 3405
Size: 1 8394
EcoRI G'AATT_C
Cuts at: 3113 5594 5704 6082 3113
Size: 2481 110 378 5426
Fragments arranged by size:
5426 2481 378 110
EcoRV GAT'ATC
Cuts at: 4929 5308 4929
Size: 379 8016
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2324 2522 3641 4939 2324
Size: 198 1119 1298 5780
Fragments arranged by size:
5780 1298 1119 198
FspI TGC'GCA
Cuts at: 4334 6901 4334
Size: 2567 5828
HaeII r_GCGC'y
Cuts at: 1678 2016 7542 1678
Size: 338 5526 2531
Fragments arranged by size:
5526 2531 338
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2672 2704 3115 3147 3482
Size: 32 135 32 1100 32 411 32 335
Cuts at: 3482 3514 4191 4223 5292 5324 5648 5680 7036
Size: 32 677 32 1069 32 324 32 1356
Cuts at: 7036 7068 7110 7142 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1356 1100 1069 677 411 335 324
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2556 2691 3378 3882 4709 5739
Size: 988 281 618 135 687 504 827 1030
Cuts at: 5739 5985 669
Size: 246 3079
Fragments arranged by size:
3079 1030 988 827 687 618 504 281
246 135
HindIII A'AGCT_T
Cuts at: 748 4593 748
Size: 3845 4550
HpaI GTT'AAC
Cuts at: 3378 3882 5985 3378
Size: 504 2103 5788
Fragments arranged by size:
5788 2103 504
KpnI G_GTAC'C
Cuts at: 5720 5720
Size: 8395
MfeI C'AATT_G
Cuts at: 2262 5994 2262
Size: 3732 4663
MluI A'CGCG_T
Cuts at: 8271 8271
Size: 8395
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2106 3023 3568 4548 5409 7509 7693 1305
Size: 801 917 545 980 861 2100 184 2007
Fragments arranged by size:
2100 2007 980 917 861 801 545 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2604 4139 10
Size: 54 1425 343 772 1535 4266
Fragments arranged by size:
4266 1535 1425 772 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2285 4655 5263 6353 6712 6871 518
Size: 648 1119 2370 608 1090 359 159 2042
Fragments arranged by size:
2370 2042 1119 1090 648 608 359 159
MspA1I CmG'CkG
Cuts at: 1606 3062 3740 4064 4451 4628 5811 6488 7636
Size: 1456 678 324 387 177 1183 677 1148
Cuts at: 7636 7881 1606
Size: 245 2120
Fragments arranged by size:
2120 1456 1183 1148 678 677 387 324
245 177
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6904
Fragments arranged by size:
6904 939 552
NdeI CA'TA_TG
Cuts at: 387 4786 4942 387
Size: 4399 156 3840
Fragments arranged by size:
4399 3840 156
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8395
NotI GC'GGCC_GC
Cuts at: 5842 5842
Size: 8395
NsiI A_TGCA'T
Cuts at: 4125 4642 4125
Size: 517 7878
NspI r_CATG'y
Cuts at: 1132 1229 2290 3376 4127 4339 4434 5463 5753
Size: 97 1061 1086 751 212 95 1029 290
Cuts at: 5753 7298 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 1086 1061 1029 751 290 212
97 95
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8395
PciI A'CATG_T
Cuts at: 3372 5459 7294 3372
Size: 2087 1835 4473
Fragments arranged by size:
4473 2087 1835
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4288 1108
Size: 3180 5215
PmeI GTTT'AAAC
Cuts at: 5699 5699
Size: 8395
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3942 3974 6464 6496 7977 8009 3942
Size: 32 2490 32 1481 32 4328
Fragments arranged by size:
4328 2490 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 5289 1613
Size: 3676 4719
PsiI TTA'TAA
Cuts at: 2897 2937 5192 5965 2897
Size: 40 2255 773 5327
Fragments arranged by size:
5327 2255 773 40
PspOMI G'GGCC_C
Cuts at: 3405 3405
Size: 8395
PspXI vC'TCGA_Gb
Cuts at: 4425 4425
Size: 8395
PstI C_TGCA'G
Cuts at: 830 2109 5424 5747 830
Size: 1279 3315 323 3478
Fragments arranged by size:
3478 3315 1279 323
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8395
PvuII CAG'CTG
Cuts at: 3062 3740 4064 4451 4628 3062
Size: 678 324 387 177 6829
Fragments arranged by size:
6829 678 387 324 177
SacI G_AGCT'C
Cuts at: 4095 4258 4095
Size: 163 8232
SalI G'TCGA_C
Cuts at: 1936 5737 1936
Size: 3801 4594
SapI GCTCTTCn'nnn_
Cuts at: 3324 3324
Size: 8395
SbfI CC_TGCA'GG
Cuts at: 5747 5747
Size: 8395
ScaI AGT'ACT
Cuts at: 2825 5418 6643 8355 2825
Size: 2593 1225 1712 2865
Fragments arranged by size:
2865 2593 1712 1225
SexAI A'CCwGG_T
Cuts at: 3875 3875
Size: 8395
SfcI C'TryA_G
Cuts at: 826 1045 2105 3081 3885 4045 4452 4494 5420
Size: 219 1060 976 804 160 407 42 926
Cuts at: 5420 5743 6878 7559 7750 826
Size: 323 1135 681 191 1471
Fragments arranged by size:
1471 1135 1060 976 926 804 681 407
323 219 191 160 42
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8395
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8395
SmaI CCC'GGG
Cuts at: 1872 5722 1872
Size: 3850 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2762 3534 4425 4486 4555 4913
Size: 197 926 819 772 891 61 69 358
Cuts at: 4913 6499 7400 7662 820
Size: 1586 901 262 1553
Fragments arranged by size:
1586 1553 926 901 891 819 772 358
262 197 69 61
SnaBI TAC'GTA
Cuts at: 493 3511 6186 493
Size: 3018 2675 2702
Fragments arranged by size:
3018 2702 2675
SpeI A'CTAG_T
Cuts at: 152 4703 5690 152
Size: 4551 987 2857
Fragments arranged by size:
4551 2857 987
SphI G_CATG'C
Cuts at: 1132 4339 4434 5753 1132
Size: 3207 95 1319 3774
Fragments arranged by size:
3774 3207 1319 95
SspI AAT'ATT
Cuts at: 5 52 2902 6319 5
Size: 47 2850 3417 2081
Fragments arranged by size:
3417 2850 2081 47
StuI AGG'CCT
Cuts at: 1517 3940 1517
Size: 2423 5972
StyI C'CwwG_G
Cuts at: 513 1065 2004 2466 3802 5082 5834 513
Size: 552 939 462 1336 1280 752 3074
Fragments arranged by size:
3074 1336 1280 939 752 552 462
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5448 6422 6439 6592 6777 7222 325
Size: 1255 3868 974 17 153 185 445 1498
Fragments arranged by size:
3868 1498 1255 974 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2823 3226 3427
Size: 274 80 33 51 1443 846 403 201
Cuts at: 3427 5271 5416 5462 6641 8353 96
Size: 1844 145 46 1179 1712 138
Fragments arranged by size:
1844 1712 1443 1179 846 403 274 201
145 138 80 51 46 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8355 8387 8355
Size: 32 8363
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8395
XbaI T'CTAG_A
Cuts at: 4084 5281 5731 4084
Size: 1197 450 6748
Fragments arranged by size:
6748 1197 450
XhoI C'TCGA_G
Cuts at: 1943 4425 1943
Size: 2482 5913
XmaI C'CCGG_G
Cuts at: 1870 5720 1870
Size: 3850 4545
XmnI GAAnn'nnTTC
Cuts at: 2307 3105 3621 3957 6524 2307
Size: 798 516 336 2567 4178
Fragments arranged by size:
4178 2567 798 516 336
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2414 6203 276
Size: 53 83 186 936 880 3789 2468
Fragments arranged by size:
3789 2468 936 880 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BsiWI
BstZ17I DraIII EcoNI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PsrI RsrII
SacII SanDI SfiI SfoI SrfI SwaI XcmI