Restriction map for
pFN21AA0820
(Circular) (Six-base) MAPSORT of: pFN21AA0820.seq Check: 8276 from: 1 to: 7282
pFN21AA0820 7282 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2841 2841
Size: 7282
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5092 278
Size: 53 83 186 936 3556 2468
Fragments arranged by size:
3556 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3780 3831 3972 4625 1937
Size: 1843 51 141 653 4594
Fragments arranged by size:
4594 1843 653 141 51
Acc65I G'GTAC_C
Cuts at: 4603 4603
Size: 7282
AclI AA'CG_TT
Cuts at: 1563 4050 5409 5782 1563
Size: 2487 1359 373 3063
Fragments arranged by size:
3063 2487 1359 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2585 2875 4080 5345 6729 6997 1632
Size: 953 290 1205 1265 1384 268 1917
Fragments arranged by size:
1917 1384 1265 1205 953 290 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7085
AflIII A'CryG_T
Cuts at: 1203 2058 2386 2388 3981 6181 7158 1203
Size: 855 328 2 1593 2200 977 1327
Fragments arranged by size:
2200 1593 1327 977 855 328 2
AgeI A'CCGG_T
Cuts at: 6113 6113
Size: 7282
AhdI GACnn_n'nnGTC
Cuts at: 6011 6011
Size: 7282
AleI CACnn'nnGTG
Cuts at: 1166 3396 1166
Size: 2230 5052
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3765 1184
Size: 2581 4701
AlwNI CAG_nnn'CTG
Cuts at: 3360 4364 5025 6597 3360
Size: 1004 661 1572 4045
Fragments arranged by size:
4045 1572 1004 661
ApaI G_GGCC'C
Cuts at: 4498 4498
Size: 7282
ApaLI G'TGCA_C
Cuts at: 5339 6495 5339
Size: 1156 6126
ApoI r'AATT_y
Cuts at: 1493 2273 2950 4066 4591 4817 4969 7078 1493
Size: 780 677 1116 525 226 152 2109 1697
Fragments arranged by size:
2109 1697 1116 780 677 525 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2364 3308 5836 160
Size: 872 1332 944 2528 1606
Fragments arranged by size:
2528 1606 1332 944 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7282
AvaI C'yCGr_G
Cuts at: 1870 1943 2147 2178 4607 1870
Size: 73 204 31 2429 4545
Fragments arranged by size:
4545 2429 204 73 31
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3985 4018 3985
Size: 33 7249
BamHI G'GATC_C
Cuts at: 4612 4979 4612
Size: 367 6915
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4234 4603 6058 618
Size: 325 217 627 2447 369 1455 1842
Fragments arranged by size:
2447 1842 1455 627 369 325 217
BanII G_rGCy'C
Cuts at: 1391 2141 4077 4399 4465 4498 4601 1391
Size: 750 1936 322 66 33 103 4072
Fragments arranged by size:
4072 1936 750 322 103 66 33
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2970 2984 3419 3483 928
Size: 971 1071 14 435 64 4727
Fragments arranged by size:
4727 1071 971 435 64 14
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5473 5507 1903
Size: 34 3536 34 3678
Fragments arranged by size:
3678 3536 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2869 3605 4013 4254 5174 6384 2869
Size: 736 408 241 920 1210 3767
Fragments arranged by size:
3767 1210 920 736 408 241
BclI T'GATC_A
Cuts at: 1548 4041 1548
Size: 2493 4789
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2762 5893 136
Size: 107 122 71 631 605 1090 3131 1525
Fragments arranged by size:
3131 1525 1090 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4009 7277 4009
Size: 3268 4014
BlpI GC'TnA_GC
Cuts at: 3501 4131 4140 4699 3501
Size: 630 9 559 6084
Fragments arranged by size:
6084 630 559 9
Bme1580I G_kGCm'C
Cuts at: 1790 4498 5343 6499 1790
Size: 2708 845 1156 2573
Fragments arranged by size:
2708 2573 1156 845
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7282
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4497 5013 5971
Size: 416 53 83 399 24 3080 516 958
Cuts at: 5971 442
Size: 1753
Fragments arranged by size:
3080 1753 958 516 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2573 1056
Size: 1517 5765
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2111 2514 3201 4227 4386 5942 1239
Size: 872 403 687 1026 159 1556 2579
Fragments arranged by size:
2579 1556 1026 872 687 403 159
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2321 2574 3821 5407 6272 6570 2321
Size: 253 1247 1586 865 298 3033
Fragments arranged by size:
3033 1586 1247 865 298 253
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3947 4513 5945 882
Size: 617 2448 566 1432 2219
Fragments arranged by size:
2448 2219 1432 617 566
BsaAI yAC'GTr
Cuts at: 493 1204 2389 2698 5073 493
Size: 711 1185 309 2375 2702
Fragments arranged by size:
2702 2375 1185 711 309
BsaBI GATnn'nnATC
Cuts at: 7276 7276
Size: 7282
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5089 5471 6106 275
Size: 53 83 186 936 3556 382 635 1451
Fragments arranged by size:
3556 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5715 6113 6387 6534 6945 5715
Size: 398 274 147 411 6052
Fragments arranged by size:
6052 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7057
Fragments arranged by size:
7057 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2039 3523 4275 4389 1186
Size: 853 1484 752 114 4079
Fragments arranged by size:
4079 1484 853 752 114
BseYI C'CCAG_C
Cuts at: 2071 3860 4439 6485 6981 2071
Size: 1789 579 2046 496 2372
Fragments arranged by size:
2372 2046 1789 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6709
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4351 4732 5493 6521 1540
Size: 275 186 2350 381 761 1028 2301
Fragments arranged by size:
2350 2301 1028 761 381 275 186
BsiHKAI G_wGCw'C
Cuts at: 2141 2148 4077 4327 5343 5428 6499 2141
Size: 7 1929 250 1016 85 1071 2924
Fragments arranged by size:
2924 1929 1071 1016 250 85 7
BsmI GAATG_Cn'
Cuts at: 4793 4886 4793
Size: 93 7189
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2141 2148 4077 4327 4399 4465 4498
Size: 399 351 7 1929 250 72 66 33
Cuts at: 4498 4601 5343 5428 6499 1391
Size: 103 742 85 1071 2174
Fragments arranged by size:
2174 1929 1071 742 399 351 250 103
85 72 66 33 7
BspEI T'CCGG_A
Cuts at: 6945 6945
Size: 7282
BspHI T'CATG_A
Cuts at: 3103 3591 5171 6901 3103
Size: 488 1580 1730 3484
Fragments arranged by size:
3484 1730 1580 488
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2841 4266 4637 844
Size: 1997 1425 371 3489
Fragments arranged by size:
3489 1997 1425 371
BsrBI CCG'CTC
Cuts at: 1394 1823 5169 1394
Size: 429 3346 3507
Fragments arranged by size:
3507 3346 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2257 2987 5777 5951 65
Size: 1183 1009 730 2790 174 1396
Fragments arranged by size:
2790 1396 1183 1009 730 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2129 5926 6113 1700
Size: 110 319 3797 187 2869
Fragments arranged by size:
3797 2869 319 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7282
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7282
BssSI C'ACGA_G
Cuts at: 2497 5342 6354 2497
Size: 2845 1012 3425
Fragments arranged by size:
3425 2845 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2243 794
Size: 1449 5833
BstBI TT'CG_AA
Cuts at: 4162 6086 4162
Size: 1924 5358
BstEII G'GTnAC_C
Cuts at: 1184 4278 1184
Size: 3094 4188
BstXI CCAn_nnnn'nTGG
Cuts at: 3340 3340
Size: 7282
BstYI r'GATC_y
Cuts at: 1972 3289 4009 4612 4979 5364 5381 6833 6919
Size: 1317 720 603 367 385 17 1452 86
Cuts at: 6919 6931 7277 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1317 720 603 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 3781 3781
Size: 7282
BtgI C'CryG_G
Cuts at: 513 1065 2004 2109 3924 513
Size: 552 939 105 1815 3871
Fragments arranged by size:
3871 1815 939 552 105
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6543
Fragments arranged by size:
6543 683 56
BtsI GCAGTG_nn'
Cuts at: 4453 4798 5592 5612 4453
Size: 345 794 20 6123
Fragments arranged by size:
6123 794 345 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7282
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7247
DraI TTT'AAA
Cuts at: 4586 4586
Size: 7282
DraIII CAC_nnn'GTG
Cuts at: 4322 4322
Size: 7282
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3449 6289 809
Size: 507 2133 2840 1802
Fragments arranged by size:
2840 2133 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2119 4215 4348
Size: 54 1425 50 275 18 289 2096 133
Cuts at: 4348 4729 5618 7247 8
Size: 381 889 1629 43
Fragments arranged by size:
2096 1629 1425 889 381 289 275 133
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4348 4729 1537
Size: 275 2536 381 4090
Fragments arranged by size:
4090 2536 381 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2688 3023 3154 3275 5212 1353
Size: 1335 335 131 121 1937 3423
Fragments arranged by size:
3423 1937 1335 335 131 121
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4544 5869 6243 6389 387
Size: 1064 138 2955 1325 374 146 1280
Fragments arranged by size:
2955 1325 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2139 4075 2139
Size: 1936 5346
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2111 2514 2585 2875 3201 4080 4227
Size: 393 479 403 71 290 326 879 147
Cuts at: 4227 4386 5345 5942 6729 6997 1239
Size: 159 959 597 787 268 1524
Fragments arranged by size:
1524 959 879 787 597 479 403 393
326 290 268 159 147 71
EcoNI CCTnn'n_nnAGG
Cuts at: 2837 4118 2837
Size: 1281 6001
EcoO109I rG'GnC_Cy
Cuts at: 2842 3269 4494 4495 2842
Size: 427 1225 1 5629
Fragments arranged by size:
5629 1225 427 1
EcoRI G'AATT_C
Cuts at: 4591 4969 4591
Size: 378 6904
EcoRV GAT'ATC
Cuts at: 2458 2458
Size: 7282
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2315 3763 4205 2315
Size: 1448 442 5392
Fragments arranged by size:
5392 1448 442
FspI TGC'GCA
Cuts at: 5788 5788
Size: 7282
HaeII r_GCGC'y
Cuts at: 1678 2072 2130 4147 6429 1678
Size: 394 58 2017 2282 2531
Fragments arranged by size:
2531 2282 2017 394 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2901 2933 3274 3306 3499
Size: 32 135 32 1329 32 341 32 193
Cuts at: 3499 3531 3849 3881 5923 5955 5997 6029 1373
Size: 32 318 32 2042 32 42 32 2626
Fragments arranged by size:
2626 2042 1329 341 318 193 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3832 4626 4872 669
Size: 988 281 1894 794 246 3079
Fragments arranged by size:
3079 1894 988 794 281 246
HindIII A'AGCT_T
Cuts at: 748 3637 748
Size: 2889 4393
HpaI GTT'AAC
Cuts at: 4872 4872
Size: 7282
KpnI G_GTAC'C
Cuts at: 4607 4607
Size: 7282
MfeI C'AATT_G
Cuts at: 3935 4881 3935
Size: 946 6336
MluI A'CGCG_T
Cuts at: 2058 7158 2058
Size: 5100 2182
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3520 6396 6580 1305
Size: 2215 2876 184 2007
Fragments arranged by size:
2876 2215 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5460
Fragments arranged by size:
5460 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3396 5240 5599 5758 518
Size: 648 2230 1844 359 159 2042
Fragments arranged by size:
2230 2042 1844 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2038 3837 4698 5375 6523 6768 1606
Size: 432 1799 861 677 1148 245 2120
Fragments arranged by size:
2120 1799 1148 861 677 432 245
NaeI GCC'GGC
Cuts at: 2131 2131
Size: 7282
NcoI C'CATG_G
Cuts at: 513 1065 2004 3924 513
Size: 552 939 1920 3871
Fragments arranged by size:
3871 1920 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7282
NgoMIV G'CCGG_C
Cuts at: 2129 2129
Size: 7282
NheI G'CTAG_C
Cuts at: 1052 2569 1052
Size: 1517 5765
NotI GC'GGCC_GC
Cuts at: 4729 4729
Size: 7282
NspI r_CATG'y
Cuts at: 1132 1229 3685 3813 3985 4640 6185 1132
Size: 97 2456 128 172 655 1545 2229
Fragments arranged by size:
2456 2229 1545 655 172 128 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7282
PciI A'CATG_T
Cuts at: 3981 6181 3981
Size: 2200 5082
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7282
PfoI T'CCnGG_A
Cuts at: 4256 4256
Size: 7282
PmeI GTTT'AAAC
Cuts at: 4586 4586
Size: 7282
PmlI CAC'GTG
Cuts at: 2389 2389
Size: 7282
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5351 5383 6864 6896 5351
Size: 32 1481 32 5737
Fragments arranged by size:
5737 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2842 2842
Size: 7282
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7282
PsiI TTA'TAA
Cuts at: 4852 4852
Size: 7282
PspOMI G'GGCC_C
Cuts at: 4494 4494
Size: 7282
PspXI vC'TCGA_Gb
Cuts at: 2178 2178
Size: 7282
PstI C_TGCA'G
Cuts at: 830 2055 2220 2838 2997 4634 830
Size: 1225 165 618 159 1637 3478
Fragments arranged by size:
3478 1637 1225 618 165 159
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7282
PvuII CAG'CTG
Cuts at: 3837 3837
Size: 7282
SacI G_AGCT'C
Cuts at: 2141 4077 2141
Size: 1936 5346
SalI G'TCGA_C
Cuts at: 1936 3830 4624 1936
Size: 1894 794 4594
Fragments arranged by size:
4594 1894 794
SbfI CC_TGCA'GG
Cuts at: 4634 4634
Size: 7282
ScaI AGT'ACT
Cuts at: 5530 7242 5530
Size: 1712 5570
SexAI A'CCwGG_T
Cuts at: 3423 3423
Size: 7282
SfcI C'TryA_G
Cuts at: 826 1045 2051 2216 2834 2903 2993 3882 4630
Size: 219 1006 165 618 69 90 889 748
Cuts at: 4630 5765 6446 6637 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1006 889 748 681 618 219
191 165 90 69
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7282
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7282
SmaI CCC'GGG
Cuts at: 1872 4609 1872
Size: 2737 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2147 2178 2300 2589 3800 5386
Size: 197 926 204 31 122 289 1211 1586
Cuts at: 5386 6287 6549 820
Size: 901 262 1553
Fragments arranged by size:
1586 1553 1211 926 901 289 262 204
197 122 31
SnaBI TAC'GTA
Cuts at: 493 5073 493
Size: 4580 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7282
SphI G_CATG'C
Cuts at: 1132 4640 1132
Size: 3508 3774
SspI AAT'ATT
Cuts at: 5 52 5206 5
Size: 47 5154 2081
Fragments arranged by size:
5154 2081 47
StuI AGG'CCT
Cuts at: 1517 4486 1517
Size: 2969 4313
StyI C'CwwG_G
Cuts at: 513 1065 2004 3690 3924 4189 4721 513
Size: 552 939 1686 234 265 532 3074
Fragments arranged by size:
3074 1686 939 552 532 265 234
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2829 5309 5326 5479 5664 6109 325
Size: 1255 1249 2480 17 153 185 445 1498
Fragments arranged by size:
2480 1498 1255 1249 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3678 5528 7240
Size: 274 80 33 51 1443 1701 1850 1712
Cuts at: 7240 96
Size: 138
Fragments arranged by size:
1850 1712 1701 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3534 3566 7242 7274 3534
Size: 32 3676 32 3542
Fragments arranged by size:
3676 3542 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7282
XbaI T'CTAG_A
Cuts at: 2662 4618 2662
Size: 1956 5326
XhoI C'TCGA_G
Cuts at: 1943 2147 2178 1943
Size: 204 31 7047
Fragments arranged by size:
7047 204 31
XmaI C'CCGG_G
Cuts at: 1870 4607 1870
Size: 2737 4545
XmnI GAAnn'nnTTC
Cuts at: 2777 3111 5411 2777
Size: 334 2300 4648
Fragments arranged by size:
4648 2300 334
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5090 276
Size: 53 83 186 936 3556 2468
Fragments arranged by size:
3556 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI Bsu36I FseI FspAI KasI NarI
NruI NsiI PacI PsrI RsrII SacII SanDI SapI
SfiI SfoI SrfI SwaI XcmI