Restriction map for
pFN21AA0810
(Circular) (Six-base) MAPSORT of: pFN21AA0810.seq Check: 4584 from: 1 to: 7141
pFN21AA0810 7141 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3178 3178
Size: 7141
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4951 278
Size: 53 83 186 936 3415 2468
Fragments arranged by size:
3415 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4397 4484 1937
Size: 2460 87 4594
Fragments arranged by size:
4594 2460 87
Acc65I G'GTAC_C
Cuts at: 2886 4113 4462 2886
Size: 1227 349 5565
Fragments arranged by size:
5565 1227 349
AclI AA'CG_TT
Cuts at: 1563 5268 5641 1563
Size: 3705 373 3063
Fragments arranged by size:
3705 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2079 2273 3143 5204 6588 6856 1632
Size: 447 194 870 2061 1384 268 1917
Fragments arranged by size:
2061 1917 1384 870 447 268 194
AfeI AGC'GCT
Cuts at: 2242 2242
Size: 7141
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6944
AflIII A'CryG_T
Cuts at: 1203 2025 2067 2322 3096 3565 6040 7017 1203
Size: 822 42 255 774 469 2475 977 1327
Fragments arranged by size:
2475 1327 977 822 774 469 255 42
AgeI A'CCGG_T
Cuts at: 5972 5972
Size: 7141
AhdI GACnn_n'nnGTC
Cuts at: 5870 5870
Size: 7141
AleI CACnn'nnGTG
Cuts at: 1166 3222 1166
Size: 2056 5085
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2609 3888 1184
Size: 1425 1279 4437
Fragments arranged by size:
4437 1425 1279
AlwNI CAG_nnn'CTG
Cuts at: 2565 2764 2855 3133 3262 3499 3685 3760 4270
Size: 199 91 278 129 237 186 75 510
Cuts at: 4270 4324 4884 6456 2565
Size: 54 560 1572 3250
Fragments arranged by size:
3250 1572 560 510 278 237 199 186
129 91 75 54
ApaI G_GGCC'C
Cuts at: 2625 2625
Size: 7141
ApaLI G'TGCA_C
Cuts at: 5198 6354 5198
Size: 1156 5985
ApoI r'AATT_y
Cuts at: 1493 4450 4676 4828 6937 1493
Size: 2957 226 152 2109 1697
Fragments arranged by size:
2957 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5695 160
Size: 872 4663 1606
Fragments arranged by size:
4663 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7141
AvaI C'yCGr_G
Cuts at: 1870 1943 2618 3270 3340 4466 1870
Size: 73 675 652 70 1126 4545
Fragments arranged by size:
4545 1126 675 652 73 70
BamHI G'GATC_C
Cuts at: 4020 4471 4838 4020
Size: 451 367 6323
Fragments arranged by size:
6323 451 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2219 2231 2685 2886 2937
Size: 325 217 627 432 12 454 201 51
Cuts at: 2937 4113 4462 5917 618
Size: 1176 349 1455 1842
Fragments arranged by size:
1842 1455 1176 627 454 432 349 325
217 201 51 12
BanII G_rGCy'C
Cuts at: 1391 2625 4460 1391
Size: 1234 1835 4072
Fragments arranged by size:
4072 1835 1234
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3578 928
Size: 971 1679 4491
Fragments arranged by size:
4491 1679 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2687 2721 2934 2968 5332 5366 1903
Size: 34 750 34 213 34 2364 34 3678
Fragments arranged by size:
3678 2364 750 213 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5033 6243 5033
Size: 1210 5931
BclI T'GATC_A
Cuts at: 1548 4288 1548
Size: 2740 4401
BfrBI ATG'CAT
Cuts at: 3064 3064
Size: 7141
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5752 136
Size: 107 122 71 631 605 4080 1525
Fragments arranged by size:
4080 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7136 7136
Size: 7141
BlpI GC'TnA_GC
Cuts at: 4558 4558
Size: 7141
Bme1580I G_kGCm'C
Cuts at: 1790 2625 5202 6358 1790
Size: 835 2577 1156 2573
Fragments arranged by size:
2577 2573 1156 835
BmgBI CAC'GTC
Cuts at: 1318 2340 2593 3097 3616 1318
Size: 1022 253 504 519 4843
Fragments arranged by size:
4843 1022 519 504 253
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2035 3061 4390
Size: 416 53 83 399 24 618 1026 1329
Cuts at: 4390 4872 5830 442
Size: 482 958 1753
Fragments arranged by size:
1753 1329 1026 958 618 482 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7141
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2823 2877 3503 3545 4184 4292 5801 1239
Size: 1584 54 626 42 639 108 1509 2579
Fragments arranged by size:
2579 1584 1509 639 626 108 54 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3982 5266 6131 6429 3982
Size: 1284 865 298 4694
Fragments arranged by size:
4694 1284 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3005 3735 5804 882
Size: 617 1506 730 2069 2219
Fragments arranged by size:
2219 2069 1506 730 617
BsaAI yAC'GTr
Cuts at: 493 1204 2070 2323 3568 3872 4284 4932 493
Size: 711 866 253 1245 304 412 648 2702
Fragments arranged by size:
2702 1245 866 711 648 412 304 253
BsaBI GATnn'nnATC
Cuts at: 2472 7135 2472
Size: 4663 2478
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2349 3845 4948 5330
Size: 53 83 186 936 816 1496 1103 382
Cuts at: 5330 5965 275
Size: 635 1451
Fragments arranged by size:
1496 1451 1103 936 816 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5574 5972 6246 6393 6804 5574
Size: 398 274 147 411 5911
Fragments arranged by size:
5911 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2537 2567 1178
Size: 30 165 30 1134 30 5752
Fragments arranged by size:
5752 1134 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7141
BseYI C'CCAG_C
Cuts at: 2872 4089 4147 6344 6840 2872
Size: 1217 58 2197 496 3173
Fragments arranged by size:
3173 2197 1217 496 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2760 1163
Size: 573 1024 5544
Fragments arranged by size:
5544 1024 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2707 4591 5352 6380 1540
Size: 275 186 706 1884 761 1028 2301
Fragments arranged by size:
2301 1884 1028 761 706 275 186
BsiHKAI G_wGCw'C
Cuts at: 2124 2598 3516 4170 5202 5287 6358 2124
Size: 474 918 654 1032 85 1071 2907
Fragments arranged by size:
2907 1071 1032 918 654 474 85
BsmI GAATG_Cn'
Cuts at: 4652 4745 4652
Size: 93 7048
BsmBI CGTCTCn'nnnn_
Cuts at: 2108 3315 2108
Size: 1207 5934
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 2598 2625 3516 4170 4460 5202
Size: 399 334 474 27 891 654 290 742
Cuts at: 5202 5287 6358 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 891 742 654 474 399 334
290 85 27
BspEI T'CCGG_A
Cuts at: 6804 6804
Size: 7141
BspHI T'CATG_A
Cuts at: 5030 6760 5030
Size: 1730 5411
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2323 2732 2987 3178 3753 4496 844
Size: 1479 409 255 191 575 743 3489
Fragments arranged by size:
3489 1479 743 575 409 255 191
BsrBI CCG'CTC
Cuts at: 1394 1823 2264 5028 1394
Size: 429 441 2764 3507
Fragments arranged by size:
3507 2764 441 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5636 5810 65
Size: 1183 4388 174 1396
Fragments arranged by size:
4388 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3575 5785 5972 1700
Size: 110 1765 2210 187 2869
Fragments arranged by size:
2869 2210 1765 187 110
BsrGI T'GTAC_A
Cuts at: 96 2028 96
Size: 1932 5209
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7141
BssSI C'ACGA_G
Cuts at: 5201 6213 5201
Size: 1012 6129
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2565 2764 3133 3499 3733 3760 794
Size: 1771 199 369 366 234 27 4175
Fragments arranged by size:
4175 1771 369 366 234 199 27
BstBI TT'CG_AA
Cuts at: 2944 5945 2944
Size: 3001 4140
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7141
BstXI CCAn_nnnn'nTGG
Cuts at: 3221 3221
Size: 7141
BstYI r'GATC_y
Cuts at: 1972 3756 4020 4471 4838 5223 5240 6692 6778
Size: 1784 264 451 367 385 17 1452 86
Cuts at: 6778 6790 7136 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1784 1452 451 385 367 346 264
86 17 12
BstZ17I GTA'TAC
Cuts at: 4398 4398
Size: 7141
BtgI C'CryG_G
Cuts at: 513 1065 2004 2150 2610 2829 3158 3220 3816
Size: 552 939 146 460 219 329 62 596
Cuts at: 3816 513
Size: 3838
Fragments arranged by size:
3838 939 596 552 460 329 219 146
62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6402
Fragments arranged by size:
6402 683 56
BtsI GCAGTG_nn'
Cuts at: 4657 5451 5471 4657
Size: 794 20 6327
Fragments arranged by size:
6327 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7141
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7106
DraI TTT'AAA
Cuts at: 4099 4445 4099
Size: 346 6795
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2809 3620 4226 6148 809
Size: 507 1493 811 606 1922 1802
Fragments arranged by size:
1922 1802 1493 811 606 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2178 2265 2826
Size: 54 1425 50 275 18 348 87 561
Cuts at: 2826 4588 5477 7106 8
Size: 1762 889 1629 43
Fragments arranged by size:
1762 1629 1425 889 561 348 275 87
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4588 1537
Size: 275 2776 4090
Fragments arranged by size:
4090 2776 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2987 5071 1353
Size: 1634 2084 3423
Fragments arranged by size:
3423 2084 1634
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5728 6102 6248 387
Size: 1064 138 4139 374 146 1280
Fragments arranged by size:
4139 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2079 2273 2823 2877 3143 3503 3545
Size: 393 447 194 550 54 266 360 42
Cuts at: 3545 4184 4292 5204 5801 6588 6856 1239
Size: 639 108 912 597 787 268 1524
Fragments arranged by size:
1524 912 787 639 597 550 447 393
360 268 266 194 108 54 42
EcoO109I rG'GnC_Cy
Cuts at: 2715 4321 2715
Size: 1606 5535
EcoRI G'AATT_C
Cuts at: 4450 4828 4450
Size: 378 6763
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4283 4283
Size: 7141
FspI TGC'GCA
Cuts at: 2768 5647 2768
Size: 2879 4262
HaeII r_GCGC'y
Cuts at: 1678 2244 3297 3891 4004 4212 6288 1678
Size: 566 1053 594 113 208 2076 2531
Fragments arranged by size:
2531 2076 1053 594 566 208 113
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4286 4318 5782 5814 5856
Size: 32 135 32 2714 32 1464 32 42
Cuts at: 5856 5888 1373
Size: 32 2626
Fragments arranged by size:
2714 2626 1464 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4485 4731 669
Size: 988 281 2547 246 3079
Fragments arranged by size:
3079 2547 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7141
HpaI GTT'AAC
Cuts at: 4731 4731
Size: 7141
KpnI G_GTAC'C
Cuts at: 2890 4117 4466 2890
Size: 1227 349 5565
Fragments arranged by size:
5565 1227 349
MfeI C'AATT_G
Cuts at: 4740 4740
Size: 7141
MluI A'CGCG_T
Cuts at: 7017 7017
Size: 7141
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6255 6439 1305
Size: 4950 184 2007
Fragments arranged by size:
4950 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2180 10
Size: 54 1425 343 348 4971
Fragments arranged by size:
4971 1425 348 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3219 3222 5099 5458 5617 518
Size: 648 2053 3 1877 359 159 2042
Fragments arranged by size:
2053 2042 1877 648 359 159 3
MspA1I CmG'CkG
Cuts at: 1606 2229 2612 3076 3523 3558 3682 3751 4027
Size: 623 383 464 447 35 124 69 276
Cuts at: 4027 4557 5234 6382 6627 1606
Size: 530 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 623 530 464 447 383
276 245 124 69 35
NcoI C'CATG_G
Cuts at: 513 1065 2004 3220 513
Size: 552 939 1216 4434
Fragments arranged by size:
4434 1216 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7141
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7141
NotI GC'GGCC_GC
Cuts at: 4588 4588
Size: 7141
NsiI A_TGCA'T
Cuts at: 3066 3066
Size: 7141
NspI r_CATG'y
Cuts at: 1132 1229 2029 2193 2575 2739 3064 4499 6044
Size: 97 800 164 382 164 325 1435 1545
Cuts at: 6044 1132
Size: 2229
Fragments arranged by size:
2229 1545 1435 800 382 325 164 164
97
PasI CC'CwG_GG
Cuts at: 1348 2197 4108 1348
Size: 849 1911 4381
Fragments arranged by size:
4381 1911 849
PciI A'CATG_T
Cuts at: 2025 6040 2025
Size: 4015 3126
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4357 1108
Size: 3249 3892
PfoI T'CCnGG_A
Cuts at: 2798 2798
Size: 7141
PmeI GTTT'AAAC
Cuts at: 4445 4445
Size: 7141
PmlI CAC'GTG
Cuts at: 2323 3568 3872 2323
Size: 1245 304 5592
Fragments arranged by size:
5592 1245 304
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5210 5242 6723 6755 5210
Size: 32 1481 32 5596
Fragments arranged by size:
5596 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2715 2715
Size: 7141
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7141
PsiI TTA'TAA
Cuts at: 4711 4711
Size: 7141
PspOMI G'GGCC_C
Cuts at: 2621 2621
Size: 7141
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2048 2080 3958 3990 2048
Size: 32 1878 32 5199
Fragments arranged by size:
5199 1878 32 32
PstI C_TGCA'G
Cuts at: 830 2379 2561 2570 2760 3138 3267 3690 3750
Size: 1549 182 9 190 378 129 423 60
Cuts at: 3750 3756 4493 830
Size: 6 737 3478
Fragments arranged by size:
3478 1549 737 423 378 190 182 129
60 9 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7141
PvuII CAG'CTG
Cuts at: 3523 3558 3682 3751 3523
Size: 35 124 69 6913
Fragments arranged by size:
6913 124 69 35
SacII CC_GC'GG
Cuts at: 2613 2613
Size: 7141
SalI G'TCGA_C
Cuts at: 1936 4483 1936
Size: 2547 4594
SanDI GG'GwC_CC
Cuts at: 2715 2715
Size: 7141
SbfI CC_TGCA'GG
Cuts at: 2379 4493 2379
Size: 2114 5027
ScaI AGT'ACT
Cuts at: 3967 5389 7101 3967
Size: 1422 1712 4007
Fragments arranged by size:
4007 1712 1422
SexAI A'CCwGG_T
Cuts at: 2933 4116 2933
Size: 1183 5958
SfcI C'TryA_G
Cuts at: 826 1045 2375 2557 2566 2756 3134 3254 3263
Size: 219 1330 182 9 190 378 120 9
Cuts at: 3263 3686 3746 3752 4489 5624 6305 6496 826
Size: 423 60 6 737 1135 681 191 1471
Fragments arranged by size:
1471 1330 1135 737 681 423 378 219
191 190 182 120 60 9 9 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7141
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7141
SmaI CCC'GGG
Cuts at: 1872 2620 4468 1872
Size: 748 1848 4545
Fragments arranged by size:
4545 1848 748
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3961 5245 6146 6408 820
Size: 197 926 2018 1284 901 262 1553
Fragments arranged by size:
2018 1553 1284 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4932 493
Size: 4439 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7141
SphI G_CATG'C
Cuts at: 1132 2193 2575 3064 4499 1132
Size: 1061 382 489 1435 3774
Fragments arranged by size:
3774 1435 1061 489 382
SspI AAT'ATT
Cuts at: 5 52 5065 5
Size: 47 5013 2081
Fragments arranged by size:
5013 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7141
StyI C'CwwG_G
Cuts at: 513 1065 2004 3220 4215 4580 513
Size: 552 939 1216 995 365 3074
Fragments arranged by size:
3074 1216 995 939 552 365
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2207 3304 3788 4161 5168 5185 5338
Size: 1255 627 1097 484 373 1007 17 153
Cuts at: 5338 5523 5968 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1097 1007 627 484 445 373
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2028 3965 5387
Size: 274 80 33 51 1443 51 1937 1422
Cuts at: 5387 7099 96
Size: 1712 138
Fragments arranged by size:
1937 1712 1443 1422 274 138 80 51
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3767 3799 7101 7133 3767
Size: 32 3302 32 3775
Fragments arranged by size:
3775 3302 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4183 1316
Size: 2867 4274
XbaI T'CTAG_A
Cuts at: 4477 4477
Size: 7141
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7141
XmaI C'CCGG_G
Cuts at: 1870 2618 4466 1870
Size: 748 1848 4545
Fragments arranged by size:
4545 1848 748
XmnI GAAnn'nnTTC
Cuts at: 3405 5270 3405
Size: 1865 5276
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4949 276
Size: 53 83 186 936 3415 2468
Fragments arranged by size:
3415 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SacII SalI SanDI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I
BsiWI Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PspXI RsrII
SacI SapI SfiI SfoI SrfI SwaI XcmI