Restriction map for
pFN21AA0806
(Circular) (Six-base) MAPSORT of: pFN21AA0806.seq Check: 1131 from: 1 to: 7900
pFN21AA0806 7900 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3981 5710 278
Size: 53 83 186 936 2445 1729 2468
Fragments arranged by size:
2468 2445 1729 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5243 1937
Size: 3306 4594
Acc65I G'GTAC_C
Cuts at: 4694 5221 4694
Size: 527 7373
AclI AA'CG_TT
Cuts at: 1563 6027 6400 1563
Size: 4464 373 3063
Fragments arranged by size:
4464 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3071 5963 7347 7615 1632
Size: 1439 2892 1384 268 1917
Fragments arranged by size:
2892 1917 1439 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2725 2765 3100 820
Size: 197 1708 40 335 5620
Fragments arranged by size:
5620 1708 335 197 40
AflIII A'CryG_T
Cuts at: 1203 6799 7776 1203
Size: 5596 977 1327
Fragments arranged by size:
5596 1327 977
AgeI A'CCGG_T
Cuts at: 6731 6731
Size: 7900
AhdI GACnn_n'nnGTC
Cuts at: 6629 6629
Size: 7900
AleI CACnn'nnGTG
Cuts at: 1166 3566 1166
Size: 2400 5500
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2146 2201 1184
Size: 962 55 6883
Fragments arranged by size:
6883 962 55
AlwNI CAG_nnn'CTG
Cuts at: 2044 4804 5643 7215 2044
Size: 2760 839 1572 2729
Fragments arranged by size:
2760 2729 1572 839
ApaLI G'TGCA_C
Cuts at: 3417 3559 5957 7113 3417
Size: 142 2398 1156 4204
Fragments arranged by size:
4204 2398 1156 142
ApoI r'AATT_y
Cuts at: 1493 2521 3025 3114 3643 3715 5209 5435 5587
Size: 1028 504 89 529 72 1494 226 152
Cuts at: 5587 7696 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1494 1028 529 504 226 152
89 72
AseI AT'TA_AT
Cuts at: 160 1032 6454 160
Size: 872 5422 1606
Fragments arranged by size:
5422 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7900
AvaI C'yCGr_G
Cuts at: 1870 1943 3942 3968 4128 4698 4749 5225 1870
Size: 73 1999 26 160 570 51 476 4545
Fragments arranged by size:
4545 1999 570 476 160 73 51 26
AvrII C'CTAG_G
Cuts at: 2020 2020
Size: 7900
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4504 4537 4686 4719 4504
Size: 33 149 33 7685
Fragments arranged by size:
7685 149 33 33
BamHI G'GATC_C
Cuts at: 5230 5597 5230
Size: 367 7533
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2014 3851 4694 5221
Size: 325 217 627 221 6 1837 843 527
Cuts at: 5221 6676 618
Size: 1455 1842
Fragments arranged by size:
1842 1837 1455 843 627 527 325 221
217 6
BanII G_rGCy'C
Cuts at: 1391 4542 5219 1391
Size: 3151 677 4072
Fragments arranged by size:
4072 3151 677
BbeI G_GCGC'C
Cuts at: 2012 2018 2012
Size: 6 7894
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3973 4518 4566 928
Size: 971 2074 545 48 4262
Fragments arranged by size:
4262 2074 971 545 48
BbvCI CC'TCA_GC
Cuts at: 2456 4779 2456
Size: 2323 5577
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6091 6125 1903
Size: 34 4154 34 3678
Fragments arranged by size:
4154 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3628 4855 5792 7002 3628
Size: 1227 937 1210 4526
Fragments arranged by size:
4526 1227 1210 937
BclI T'GATC_A
Cuts at: 1548 4795 1548
Size: 3247 4653
BfrBI ATG'CAT
Cuts at: 2921 2921
Size: 7900
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6511 136
Size: 107 122 71 631 605 4839 1525
Fragments arranged by size:
4839 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2634 2729 7895 2634
Size: 95 5166 2639
Fragments arranged by size:
5166 2639 95
BlpI GC'TnA_GC
Cuts at: 5317 5317
Size: 7900
Bme1580I G_kGCm'C
Cuts at: 1790 3421 3563 5198 5961 7117 1790
Size: 1631 142 1635 763 1156 2573
Fragments arranged by size:
2573 1635 1631 1156 763 142
BmgBI CAC'GTC
Cuts at: 1318 3558 3631 3961 1318
Size: 2240 73 330 5257
Fragments arranged by size:
5257 2240 330 73
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5631 6589 442
Size: 416 53 83 399 24 4214 958 1753
Fragments arranged by size:
4214 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3437 1056
Size: 2381 5519
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2144 4108 2144
Size: 1964 5936
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2221 2408 2462 3690 4130 4182 4726 6560
Size: 982 187 54 1228 440 52 544 1834
Cuts at: 6560 1239
Size: 2579
Fragments arranged by size:
2579 1834 1228 982 544 440 187 54
52
Bpu10I CC'TnA_GC
Cuts at: 2456 4779 2456
Size: 2323 5577
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3260 3476 6025 6890 7188 3260
Size: 216 2549 865 298 3972
Fragments arranged by size:
3972 2549 865 298 216
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2132 4058 6563 882
Size: 617 633 1926 2505 2219
Fragments arranged by size:
2505 2219 1926 633 617
BsaAI yAC'GTr
Cuts at: 493 1204 5691 493
Size: 711 4487 2702
Fragments arranged by size:
4487 2702 711
BsaBI GATnn'nnATC
Cuts at: 7894 7894
Size: 7900
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2015 2025 3978
Size: 53 83 186 936 476 6 10 1953
Cuts at: 3978 5707 6089 6724 275
Size: 1729 382 635 1451
Fragments arranged by size:
1953 1729 1451 936 635 476 382 186
83 53 10 6
BsaWI w'CCGG_w
Cuts at: 6333 6731 7005 7152 7563 6333
Size: 398 274 147 411 6670
Fragments arranged by size:
6670 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2024 2054 2406 2436 4724
Size: 30 165 30 621 30 352 30 2288
Cuts at: 4724 4754 1178
Size: 30 4324
Fragments arranged by size:
4324 2288 621 352 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2048 2090 4550 4796 1186
Size: 862 42 2460 246 4290
Fragments arranged by size:
4290 2460 862 246 42
BseYI C'CCAG_C
Cuts at: 2044 4286 4562 5040 7103 7599 2044
Size: 2242 276 478 2063 496 2345
Fragments arranged by size:
2345 2242 2063 496 478 276
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3876 1163
Size: 573 2140 5187
Fragments arranged by size:
5187 2140 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5350 6111 7139 1540
Size: 275 186 3349 761 1028 2301
Fragments arranged by size:
3349 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3421 3563 4542 5961 6046 7117 3421
Size: 142 979 1419 85 1071 4204
Fragments arranged by size:
4204 1419 1071 979 142 85
BsmI GAATG_Cn'
Cuts at: 2154 2899 3218 5411 5504 2154
Size: 745 319 2193 93 4550
Fragments arranged by size:
4550 2193 745 319 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4187 4187
Size: 7900
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3421 3563 4542 5198 5219 5961 6046
Size: 399 1631 142 979 656 21 742 85
Cuts at: 6046 7117 1391
Size: 1071 2174
Fragments arranged by size:
2174 1631 1071 979 742 656 399 142
85 21
BspEI T'CCGG_A
Cuts at: 7563 7563
Size: 7900
BspHI T'CATG_A
Cuts at: 5789 7519 5789
Size: 1730 6170
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2114 4307 5255 844
Size: 1270 2193 948 3489
Fragments arranged by size:
3489 2193 1270 948
BsrBI CCG'CTC
Cuts at: 1394 1823 2096 4971 5787 1394
Size: 429 273 2875 816 3507
Fragments arranged by size:
3507 2875 816 429 273
BsrDI GCAATG_nn'
Cuts at: 65 1248 2082 4979 6395 6569 65
Size: 1183 834 2897 1416 174 1396
Fragments arranged by size:
2897 1416 1396 1183 834 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2011 6544 6731 1700
Size: 110 201 4533 187 2869
Fragments arranged by size:
4533 2869 201 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7900
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7900
BssSI C'ACGA_G
Cuts at: 2832 5960 6972 2832
Size: 3128 1012 3760
Fragments arranged by size:
3760 3128 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3964 4645 794
Size: 3170 681 4049
Fragments arranged by size:
4049 3170 681
BstBI TT'CG_AA
Cuts at: 6704 6704
Size: 7900
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7900
BstXI CCAn_nnnn'nTGG
Cuts at: 4816 4816
Size: 7900
BstYI r'GATC_y
Cuts at: 1972 2634 2729 3460 3832 5230 5597 5982 5999
Size: 662 95 731 372 1398 367 385 17
Cuts at: 5999 7451 7537 7549 7895 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1398 731 662 385 372 367
346 95 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3855 513
Size: 552 939 1851 4558
Fragments arranged by size:
4558 1851 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7161
Fragments arranged by size:
7161 683 56
BtsI GCAGTG_nn'
Cuts at: 3561 3573 3583 5416 6210 6230 3561
Size: 12 10 1833 794 20 5231
Fragments arranged by size:
5231 1833 794 20 12 10
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7900
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7865
DraI TTT'AAA
Cuts at: 3309 5204 3309
Size: 1895 6005
DraIII CAC_nnn'GTG
Cuts at: 3600 3600
Size: 7900
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3041 3162 6907 809
Size: 507 1725 121 3745 1802
Fragments arranged by size:
3745 1802 1725 507 121
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3858 4333 5347
Size: 54 1425 50 275 18 2028 475 1014
Cuts at: 5347 6236 7865 8
Size: 889 1629 43
Fragments arranged by size:
2028 1629 1425 1014 889 475 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5347 1537
Size: 275 3535 4090
Fragments arranged by size:
4090 3535 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2084 4381 5830 1353
Size: 731 2297 1449 3423
Fragments arranged by size:
3423 2297 1449 731
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6487 6861 7007 387
Size: 1064 138 4898 374 146 1280
Fragments arranged by size:
4898 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4540 4540
Size: 7900
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2221 2408 2462 3071 3690 4130 4182
Size: 393 589 187 54 609 619 440 52
Cuts at: 4182 4726 5963 6560 7347 7615 1239
Size: 544 1237 597 787 268 1524
Fragments arranged by size:
1524 1237 787 619 609 597 589 544
440 393 268 187 54 52
EcoNI CCTnn'n_nnAGG
Cuts at: 5131 5131
Size: 7900
EcoO109I rG'GnC_Cy
Cuts at: 4746 5083 4746
Size: 337 7563
EcoRI G'AATT_C
Cuts at: 5209 5587 5209
Size: 378 7522
FspI TGC'GCA
Cuts at: 6406 6406
Size: 7900
HaeII r_GCGC'y
Cuts at: 1678 2012 2018 2210 7047 1678
Size: 334 6 192 4837 2531
Fragments arranged by size:
4837 2531 334 192 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2444 2476 2558 2590 3794
Size: 32 135 32 872 32 82 32 1204
Cuts at: 3794 3826 6541 6573 6615 6647 1373
Size: 32 2715 32 42 32 2626
Fragments arranged by size:
2715 2626 1204 872 135 82 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5244 5490 669
Size: 988 281 3306 246 3079
Fragments arranged by size:
3306 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7900
HpaI GTT'AAC
Cuts at: 5490 5490
Size: 7900
KasI G'GCGC_C
Cuts at: 2008 2014 2008
Size: 6 7894
KpnI G_GTAC'C
Cuts at: 4698 5225 4698
Size: 527 7373
MfeI C'AATT_G
Cuts at: 4366 5499 4366
Size: 1133 6767
MluI A'CGCG_T
Cuts at: 7776 7776
Size: 7900
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7014 7198 1305
Size: 5709 184 2007
Fragments arranged by size:
5709 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3860 4335 10
Size: 54 1425 343 2028 475 3575
Fragments arranged by size:
3575 2028 1425 475 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3545 3566 4401 4439 4964 5076 5858
Size: 648 2379 21 835 38 525 112 782
Cuts at: 5858 6217 6376 518
Size: 359 159 2042
Fragments arranged by size:
2379 2042 835 782 648 525 359 159
112 38 21
MspA1I CmG'CkG
Cuts at: 1606 2746 2971 4987 5316 5993 7141 7386 1606
Size: 1140 225 2016 329 677 1148 245 2120
Fragments arranged by size:
2120 2016 1148 1140 677 329 245 225
NaeI GCC'GGC
Cuts at: 2013 2013
Size: 7900
NarI GG'CG_CC
Cuts at: 2009 2015 2009
Size: 6 7894
NcoI C'CATG_G
Cuts at: 513 1065 2004 3855 513
Size: 552 939 1851 4558
Fragments arranged by size:
4558 1851 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7900
NgoMIV G'CCGG_C
Cuts at: 2011 2011
Size: 7900
NheI G'CTAG_C
Cuts at: 1052 3433 1052
Size: 2381 5519
NotI GC'GGCC_GC
Cuts at: 5347 5347
Size: 7900
NsiI A_TGCA'T
Cuts at: 2923 2923
Size: 7900
NspI r_CATG'y
Cuts at: 1132 1229 2506 3958 5258 6803 1132
Size: 97 1277 1452 1300 1545 2229
Fragments arranged by size:
2229 1545 1452 1300 1277 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7900
PciI A'CATG_T
Cuts at: 6799 6799
Size: 7900
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2662 3600 1108
Size: 1554 938 5408
Fragments arranged by size:
5408 1554 938
PfoI T'CCnGG_A
Cuts at: 2028 2163 2241 4742 2028
Size: 135 78 2501 5186
Fragments arranged by size:
5186 2501 135 78
PmeI GTTT'AAAC
Cuts at: 5204 5204
Size: 7900
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5969 6001 7482 7514 5969
Size: 32 1481 32 6355
Fragments arranged by size:
6355 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4746 4746
Size: 7900
PshAI GACnn'nnGTC
Cuts at: 1613 2172 1613
Size: 559 7341
PsiI TTA'TAA
Cuts at: 5470 5470
Size: 7900
PstI C_TGCA'G
Cuts at: 830 2174 3568 3880 4243 4555 5252 830
Size: 1344 1394 312 363 312 697 3478
Fragments arranged by size:
3478 1394 1344 697 363 312 312
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7900
PvuII CAG'CTG
Cuts at: 2971 2971
Size: 7900
SacI G_AGCT'C
Cuts at: 4542 4542
Size: 7900
SalI G'TCGA_C
Cuts at: 1936 5242 1936
Size: 3306 4594
SbfI CC_TGCA'GG
Cuts at: 5252 5252
Size: 7900
ScaI AGT'ACT
Cuts at: 6148 7860 6148
Size: 1712 6188
SfcI C'TryA_G
Cuts at: 826 1045 2170 3564 3876 4021 4239 4516 4551
Size: 219 1125 1394 312 145 218 277 35
Cuts at: 4551 4609 5248 6383 7064 7255 826
Size: 58 639 1135 681 191 1471
Fragments arranged by size:
1471 1394 1135 1125 681 639 312 277
219 218 191 145 58 35
SfoI GGC'GCC
Cuts at: 2010 2016 2010
Size: 6 7894
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7900
SgrAI Cr'CCGG_yG
Cuts at: 1700 2011 1700
Size: 311 7589
SmaI CCC'GGG
Cuts at: 1872 4700 5227 1872
Size: 2828 527 4545
Fragments arranged by size:
4545 2828 527
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2725 2765 3100 3239 3491 3942
Size: 197 926 782 40 335 139 252 451
Cuts at: 3942 6004 6905 7167 820
Size: 2062 901 262 1553
Fragments arranged by size:
2062 1553 926 901 782 451 335 262
252 197 139 40
SnaBI TAC'GTA
Cuts at: 493 5691 493
Size: 5198 2702
SpeI A'CTAG_T
Cuts at: 152 3689 152
Size: 3537 4363
SphI G_CATG'C
Cuts at: 1132 2506 3958 5258 1132
Size: 1374 1452 1300 3774
Fragments arranged by size:
3774 1452 1374 1300
SspI AAT'ATT
Cuts at: 5 52 2368 5824 5
Size: 47 2316 3456 2081
Fragments arranged by size:
3456 2316 2081 47
StuI AGG'CCT
Cuts at: 1517 5019 1517
Size: 3502 4398
StyI C'CwwG_G
Cuts at: 513 1065 2004 2020 2547 2710 3855 4318 4579
Size: 552 939 16 527 163 1145 463 261
Cuts at: 4579 5339 513
Size: 760 3074
Fragments arranged by size:
3074 1145 939 760 552 527 463 261
163 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3601 5927 5944 6097 6282 6727 325
Size: 1255 2021 2326 17 153 185 445 1498
Fragments arranged by size:
2326 2021 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4846 6146 7858
Size: 274 80 33 51 1443 2869 1300 1712
Cuts at: 7858 96
Size: 138
Fragments arranged by size:
2869 1712 1443 1300 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2406 2438 4315 4347 7860 7892 2406
Size: 32 1877 32 3513 32 2414
Fragments arranged by size:
3513 2414 1877 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4804 1316
Size: 3488 4412
XbaI T'CTAG_A
Cuts at: 2057 3247 5236 2057
Size: 1190 1989 4721
Fragments arranged by size:
4721 1989 1190
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4388 4388
Size: 7900
XhoI C'TCGA_G
Cuts at: 1943 3942 1943
Size: 1999 5901
XmaI C'CCGG_G
Cuts at: 1870 4698 5225 1870
Size: 2828 527 4545
Fragments arranged by size:
4545 2828 527
XmnI GAAnn'nnTTC
Cuts at: 3218 3318 6029 3218
Size: 100 2711 5089
Fragments arranged by size:
5089 2711 100
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3979 5708 276
Size: 53 83 186 936 2445 1729 2468
Fragments arranged by size:
2468 2445 1729 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BsiWI BstZ17I Bsu36I
EcoRV FalI FseI FspAI NruI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SrfI SwaI