Restriction map for
pFN21AA0804
(Circular) (Six-base) MAPSORT of: pFN21AA0804.seq Check: 2833 from: 1 to: 8983
pFN21AA0804 8983 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6793 278
Size: 53 83 186 936 5257 2468
Fragments arranged by size:
5257 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3336 4053 4681 6326 1937
Size: 1399 717 628 1645 4594
Fragments arranged by size:
4594 1645 1399 717 628
Acc65I G'GTAC_C
Cuts at: 6304 6304
Size: 8983
AclI AA'CG_TT
Cuts at: 1563 7110 7483 1563
Size: 5547 373 3063
Fragments arranged by size:
5547 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4251 4931 4967 5517 5525 7046 8430 8698
Size: 2619 680 36 550 8 1521 1384 268
Cuts at: 8698 1632
Size: 1917
Fragments arranged by size:
2619 1917 1521 1384 680 550 268 36
8
AfeI AGC'GCT
Cuts at: 3960 3960
Size: 8983
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8786
AflIII A'CryG_T
Cuts at: 1203 3169 7882 8859 1203
Size: 1966 4713 977 1327
Fragments arranged by size:
4713 1966 1327 977
AgeI A'CCGG_T
Cuts at: 7814 7814
Size: 8983
AhdI GACnn_n'nnGTC
Cuts at: 4628 7712 4628
Size: 3084 5899
AleI CACnn'nnGTG
Cuts at: 1166 3242 1166
Size: 2076 6907
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3260 1184
Size: 2076 6907
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2126 2158 3111 3143 4647 4679 2126
Size: 32 953 32 1504 32 6430
Fragments arranged by size:
6430 1504 953 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3391 5464 6068 6726 8298 3391
Size: 2073 604 658 1572 4076
Fragments arranged by size:
4076 2073 1572 658 604
ApaLI G'TGCA_C
Cuts at: 7040 8196 7040
Size: 1156 7827
ApoI r'AATT_y
Cuts at: 1493 2108 2424 3229 3820 4073 4268 4358 4465
Size: 615 316 805 591 253 195 90 107
Cuts at: 4465 4580 4719 4727 5006 5386 5888 5959 6292
Size: 115 139 8 279 380 502 71 333
Cuts at: 6292 6518 6670 8779 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 805 615 591 502 380 333
316 279 253 226 195 152 139 115
107 90 71 8
AseI AT'TA_AT
Cuts at: 160 1032 3941 7537 160
Size: 872 2909 3596 1606
Fragments arranged by size:
3596 2909 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8983
AvaI C'yCGr_G
Cuts at: 1870 1943 6308 1870
Size: 73 4365 4545
Fragments arranged by size:
4545 4365 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5343 5376 5343
Size: 33 8950
BamHI G'GATC_C
Cuts at: 2260 3037 5326 5584 6037 6076 6313 6680 2260
Size: 777 2289 258 453 39 237 367 4563
Fragments arranged by size:
4563 2289 777 453 367 258 237 39
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3739 5249 6304 7759 618
Size: 325 217 627 1952 1510 1055 1455 1842
Fragments arranged by size:
1952 1842 1510 1455 1055 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3679 6302 1391
Size: 2288 2623 4072
Fragments arranged by size:
4072 2623 2288
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5012 928
Size: 971 3113 4899
Fragments arranged by size:
4899 3113 971
BbvCI CC'TCA_GC
Cuts at: 3488 3488
Size: 8983
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7174 7208 1903
Size: 34 5237 34 3678
Fragments arranged by size:
5237 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2500 6875 8085 2500
Size: 4375 1210 3398
Fragments arranged by size:
4375 3398 1210
BclI T'GATC_A
Cuts at: 1548 2539 4567 6100 1548
Size: 991 2028 1533 4431
Fragments arranged by size:
4431 2028 1533 991
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7594 136
Size: 107 122 71 631 605 5922 1525
Fragments arranged by size:
5922 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3315 3447 4234 5701 8978 3315
Size: 132 787 1467 3277 3320
Fragments arranged by size:
3320 3277 1467 787 132
BlpI GC'TnA_GC
Cuts at: 6400 6400
Size: 8983
Bme1580I G_kGCm'C
Cuts at: 1790 3692 7044 8200 1790
Size: 1902 3352 1156 2573
Fragments arranged by size:
3352 2573 1902 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8983
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6714 7672 442
Size: 416 53 83 399 24 5297 958 1753
Fragments arranged by size:
5297 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8983
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5347 5347
Size: 8983
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2159 2709 3180 3390 3517 4674 6115 7643
Size: 920 550 471 210 127 1157 1441 1528
Cuts at: 7643 1239
Size: 2579
Fragments arranged by size:
2579 1528 1441 1157 920 550 471 210
127
Bpu10I CC'TnA_GC
Cuts at: 3488 3488
Size: 8983
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2169 2276 2617 3875 5171 7108 7973 8271 2169
Size: 107 341 1258 1296 1937 865 298 2881
Fragments arranged by size:
2881 1937 1296 1258 865 341 298 107
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5357 7646 882
Size: 617 3858 2289 2219
Fragments arranged by size:
3858 2289 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4781 6774 493
Size: 711 3577 1993 2702
Fragments arranged by size:
3577 2702 1993 711
BsaBI GATnn'nnATC
Cuts at: 2544 2718 8977 2544
Size: 174 6259 2550
Fragments arranged by size:
6259 2550 174
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5800 6790 7172 7807
Size: 53 83 186 936 4267 990 382 635
Cuts at: 7807 275
Size: 1451
Fragments arranged by size:
4267 1451 990 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3639 4689 7416 7814 8088 8235 8646 3639
Size: 1050 2727 398 274 147 411 3976
Fragments arranged by size:
3976 2727 1050 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2784 2814 3111 3141 3363
Size: 30 165 30 1381 30 297 30 222
Cuts at: 3363 3393 4589 4619 4647 4677 4847 4877 1178
Size: 30 1196 30 28 30 170 30 5284
Fragments arranged by size:
5284 1381 1196 297 222 170 165 30
30 30 30 30 30 30 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2187 3132 3505 4104 4107 4868 5029 6134
Size: 1001 945 373 599 3 761 161 1105
Cuts at: 6134 1186
Size: 4035
Fragments arranged by size:
4035 1105 1001 945 761 599 373 161
3
BseYI C'CCAG_C
Cuts at: 8186 8682 8186
Size: 496 8487
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4869 1163
Size: 573 3133 5277
Fragments arranged by size:
5277 3133 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6433 7194 8222 1540
Size: 275 186 4432 761 1028 2301
Fragments arranged by size:
4432 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3249 3679 6211 7044 7129 8200 3249
Size: 430 2532 833 85 1071 4032
Fragments arranged by size:
4032 2532 1071 833 430 85
BsmI GAATG_Cn'
Cuts at: 2902 4129 4285 5882 6494 6587 2902
Size: 1227 156 1597 612 93 5298
Fragments arranged by size:
5298 1597 1227 612 156 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5791 6119 5791
Size: 328 8655
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3249 3679 3692 6211 6302 7044 7129
Size: 399 1459 430 13 2519 91 742 85
Cuts at: 7129 8200 1391
Size: 1071 2174
Fragments arranged by size:
2519 2174 1459 1071 742 430 399 91
85 13
BspEI T'CCGG_A
Cuts at: 4689 8646 4689
Size: 3957 5026
BspHI T'CATG_A
Cuts at: 5203 6872 8602 5203
Size: 1669 1730 5584
Fragments arranged by size:
5584 1730 1669
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3991 4190 4618 6338 844
Size: 3147 199 428 1720 3489
Fragments arranged by size:
3489 3147 1720 428 199
BsrBI CCG'CTC
Cuts at: 1394 1823 6870 1394
Size: 429 5047 3507
Fragments arranged by size:
5047 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4107 4887 5367 5451 7478 7652 65
Size: 1183 2859 780 480 84 2027 174 1396
Fragments arranged by size:
2859 2027 1396 1183 780 480 174 84
BsrFI r'CCGG_y
Cuts at: 1700 1810 7627 7814 1700
Size: 110 5817 187 2869
Fragments arranged by size:
5817 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3967 96
Size: 3871 5112
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8983
BssSI C'ACGA_G
Cuts at: 4901 7043 8055 4901
Size: 2142 1012 5829
Fragments arranged by size:
5829 2142 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4205 5549 794
Size: 3411 1344 4228
Fragments arranged by size:
4228 3411 1344
BstBI TT'CG_AA
Cuts at: 7787 7787
Size: 8983
BstEII G'GTnAC_C
Cuts at: 1184 5737 1184
Size: 4553 4430
BstXI CCAn_nnnn'nTGG
Cuts at: 4952 4952
Size: 8983
BstYI r'GATC_y
Cuts at: 1972 2260 3037 3315 3447 3781 4234 5326 5584
Size: 288 777 278 132 334 453 1092 258
Cuts at: 5584 5701 6037 6076 6313 6680 7065 7082 8534
Size: 117 336 39 237 367 385 17 1452
Cuts at: 8534 8620 8632 8978 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1092 777 453 385 367 346
336 334 288 278 258 237 132 117
86 39 17 12
Bsu36I CC'TnA_GG
Cuts at: 4305 4305
Size: 8983
BtgI C'CryG_G
Cuts at: 513 1065 2004 2955 513
Size: 552 939 951 6541
Fragments arranged by size:
6541 951 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8244
Fragments arranged by size:
8244 683 56
BtsI GCAGTG_nn'
Cuts at: 2511 3053 6042 6499 7293 7313 2511
Size: 542 2989 457 794 20 4181
Fragments arranged by size:
4181 2989 794 542 457 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8983
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8948
DraI TTT'AAA
Cuts at: 4396 5529 6287 4396
Size: 1133 758 7092
Fragments arranged by size:
7092 1133 758
DraIII CAC_nnn'GTG
Cuts at: 2673 2673
Size: 8983
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7990 809
Size: 507 6674 1802
Fragments arranged by size:
6674 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2682 2952 3028
Size: 54 1425 50 275 18 852 270 76
Cuts at: 3028 3828 5839 6430 7319 8948 8
Size: 800 2011 591 889 1629 43
Fragments arranged by size:
2011 1629 1425 889 852 800 591 275
270 76 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6430 1537
Size: 275 4618 4090
Fragments arranged by size:
4618 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2061 4668 6913 1353
Size: 708 2607 2245 3423
Fragments arranged by size:
3423 2607 2245 708
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7570 7944 8090 387
Size: 1064 138 5981 374 146 1280
Fragments arranged by size:
5981 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3677 3677
Size: 8983
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2159 2709 3180 3390 3517 4251 4674
Size: 393 527 550 471 210 127 734 423
Cuts at: 4674 4931 4967 5517 5525 6115 7046 7643 8430
Size: 257 36 550 8 590 931 597 787
Cuts at: 8430 8698 1239
Size: 268 1524
Fragments arranged by size:
1524 931 787 734 597 590 550 550
527 471 423 393 268 257 210 127
36 8
EcoO109I rG'GnC_Cy
Cuts at: 4573 4630 5419 4573
Size: 57 789 8137
Fragments arranged by size:
8137 789 57
EcoRI G'AATT_C
Cuts at: 3820 4465 4580 6292 6670 3820
Size: 645 115 1712 378 6133
Fragments arranged by size:
6133 1712 645 378 115
EcoRV GAT'ATC
Cuts at: 3979 3979
Size: 8983
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2117 3551 5624 2117
Size: 1434 2073 5476
Fragments arranged by size:
5476 2073 1434
FspI TGC'GCA
Cuts at: 7489 7489
Size: 8983
HaeII r_GCGC'y
Cuts at: 1678 2601 3962 8130 1678
Size: 923 1361 4168 2531
Fragments arranged by size:
4168 2531 1361 923
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2282 2297 2314 2329 3496
Size: 32 135 32 710 15 17 15 1167
Cuts at: 3496 3528 4589 4614 4621 4646 4847 4879 5356
Size: 32 1061 25 7 25 201 32 477
Cuts at: 5356 5388 5440 5472 5721 5753 5756 5788 7624
Size: 32 52 32 249 32 3 32 1836
Cuts at: 7624 7656 7698 7730 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1836 1167 1061 710 477 249 201
135 52 42 32 32 32 32 32
32 32 32 32 32 25 25 17
15 15 7 3
HincII GTy'rAC
Cuts at: 669 1657 1938 4682 6327 6573 669
Size: 988 281 2744 1645 246 3079
Fragments arranged by size:
3079 2744 1645 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2094 2550 748
Size: 1346 456 7181
Fragments arranged by size:
7181 1346 456
HpaI GTT'AAC
Cuts at: 6573 6573
Size: 8983
KpnI G_GTAC'C
Cuts at: 6308 6308
Size: 8983
MfeI C'AATT_G
Cuts at: 2481 6582 2481
Size: 4101 4882
MluI A'CGCG_T
Cuts at: 8859 8859
Size: 8983
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2225 2563 2789 3382 8097 8281 1305
Size: 920 338 226 593 4715 184 2007
Fragments arranged by size:
4715 2007 920 593 338 226 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2684 2954 3830 5841 10
Size: 54 1425 343 852 270 876 2011 3152
Fragments arranged by size:
3152 2011 1425 876 852 343 270 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2028 2123 2301 3242 4691 4752 5307
Size: 648 862 95 178 941 1449 61 555
Cuts at: 5307 6941 7300 7459 518
Size: 1634 359 159 2042
Fragments arranged by size:
2042 1634 1449 941 862 648 555 359
178 159 95 61
MspA1I CmG'CkG
Cuts at: 1606 2450 2790 3331 6250 6399 7076 8224 8469
Size: 844 340 541 2919 149 677 1148 245
Cuts at: 8469 1606
Size: 2120
Fragments arranged by size:
2919 2120 1148 844 677 541 340 245
149
NcoI C'CATG_G
Cuts at: 513 1065 2004 2955 513
Size: 552 939 951 6541
Fragments arranged by size:
6541 951 939 552
NdeI CA'TA_TG
Cuts at: 387 3972 387
Size: 3585 5398
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8983
NotI GC'GGCC_GC
Cuts at: 6430 6430
Size: 8983
NspI r_CATG'y
Cuts at: 1132 1229 3173 3275 5916 6341 7886 1132
Size: 97 1944 102 2641 425 1545 2229
Fragments arranged by size:
2641 2229 1944 1545 425 102 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8983
PciI A'CATG_T
Cuts at: 3169 7882 3169
Size: 4713 4270
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3391 3715 5594 5809 6194 1108
Size: 2283 324 1879 215 385 3897
Fragments arranged by size:
3897 2283 1879 385 324 215
PfoI T'CCnGG_A
Cuts at: 2725 4837 2725
Size: 2112 6871
PmeI GTTT'AAAC
Cuts at: 6287 6287
Size: 8983
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2028 2060 3111 3143 4647 4679 7052 7084 8565
Size: 32 1051 32 1504 32 2373 32 1481
Cuts at: 8565 8597 2028
Size: 32 2414
Fragments arranged by size:
2414 2373 1504 1481 1051 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 4573 4630 4573
Size: 57 8926
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8983
PsiI TTA'TAA
Cuts at: 6553 6553
Size: 8983
PstI C_TGCA'G
Cuts at: 830 2449 3116 3774 4650 6255 6335 830
Size: 1619 667 658 876 1605 80 3478
Fragments arranged by size:
3478 1619 1605 876 667 658 80
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8983
PvuII CAG'CTG
Cuts at: 2450 3331 6250 2450
Size: 881 2919 5183
Fragments arranged by size:
5183 2919 881
SacI G_AGCT'C
Cuts at: 3679 3679
Size: 8983
SalI G'TCGA_C
Cuts at: 1936 4680 6325 1936
Size: 2744 1645 4594
Fragments arranged by size:
4594 2744 1645
SapI GCTCTTCn'nnn_
Cuts at: 2061 2061
Size: 8983
SbfI CC_TGCA'GG
Cuts at: 6335 6335
Size: 8983
ScaI AGT'ACT
Cuts at: 7231 8943 7231
Size: 1712 7271
SexAI A'CCwGG_T
Cuts at: 4257 4989 4257
Size: 732 8251
SfcI C'TryA_G
Cuts at: 826 1045 2445 2475 3112 3770 4646 6181 6251
Size: 219 1400 30 637 658 876 1535 70
Cuts at: 6251 6331 7466 8147 8338 826
Size: 80 1135 681 191 1471
Fragments arranged by size:
1535 1471 1400 1135 876 681 658 637
219 191 80 70 30
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8983
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8983
SmaI CCC'GGG
Cuts at: 1872 6310 1872
Size: 4438 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2184 2255 2632 3854 5150 7087
Size: 197 926 241 71 377 1222 1296 1937
Cuts at: 7087 7988 8250 820
Size: 901 262 1553
Fragments arranged by size:
1937 1553 1296 1222 926 901 377 262
241 197 71
SnaBI TAC'GTA
Cuts at: 493 6774 493
Size: 6281 2702
SpeI A'CTAG_T
Cuts at: 152 2576 152
Size: 2424 6559
SphI G_CATG'C
Cuts at: 1132 6341 1132
Size: 5209 3774
SspI AAT'ATT
Cuts at: 5 52 2535 2739 6907 5
Size: 47 2483 204 4168 2081
Fragments arranged by size:
4168 2483 2081 204 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8983
StyI C'CwwG_G
Cuts at: 513 1065 2004 2871 2955 3697 4216 4245 5030
Size: 552 939 867 84 742 519 29 785
Cuts at: 5030 6422 513
Size: 1392 3074
Fragments arranged by size:
3074 1392 939 867 785 742 552 519
84 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4958 5725 7010 7027 7180 7365 7810
Size: 1255 3378 767 1285 17 153 185 445
Cuts at: 7810 325
Size: 1498
Fragments arranged by size:
3378 1498 1285 1255 767 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3079 3967 5790
Size: 274 80 33 51 1443 1102 888 1823
Cuts at: 5790 7229 8941 96
Size: 1439 1712 138
Fragments arranged by size:
1823 1712 1443 1439 1102 888 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6072 6104 8943 8975 6072
Size: 32 2839 32 6080
Fragments arranged by size:
6080 2839 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2562 1316
Size: 1246 7737
XbaI T'CTAG_A
Cuts at: 2089 2849 6319 2089
Size: 760 3470 4753
Fragments arranged by size:
4753 3470 760
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2677 3060 2677
Size: 383 8600
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8983
XmaI C'CCGG_G
Cuts at: 1870 6308 1870
Size: 4438 4545
XmnI GAAnn'nnTTC
Cuts at: 4097 5040 5175 6069 7112 4097
Size: 943 135 894 1043 5968
Fragments arranged by size:
5968 1043 943 894 135
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6791 276
Size: 53 83 186 936 5257 2468
Fragments arranged by size:
5257 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BbeI BfrBI BsiWI BstZ17I
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SfiI SfoI SrfI SwaI