Restriction map for
pFN21AA0802
(Circular) (Six-base) MAPSORT of: pFN21AA0802.seq Check: 6778 from: 1 to: 9340
pFN21AA0802 9340 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2844 3589 5495 5580 2844
Size: 745 1906 85 6604
Fragments arranged by size:
6604 1906 745 85
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3281 6113 7150 278
Size: 53 83 186 936 1745 2832 1037 2468
Fragments arranged by size:
2832 2468 1745 1037 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5487 6683 1937
Size: 3550 1196 4594
Fragments arranged by size:
4594 3550 1196
Acc65I G'GTAC_C
Cuts at: 5346 6661 5346
Size: 1315 8025
AclI AA'CG_TT
Cuts at: 1563 4803 7467 7840 1563
Size: 3240 2664 373 3063
Fragments arranged by size:
3240 3063 2664 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2645 3038 3359 3865 4934 5895 7403 8787
Size: 1013 393 321 506 1069 961 1508 1384
Cuts at: 8787 9055 1632
Size: 268 1917
Fragments arranged by size:
1917 1508 1384 1069 1013 961 506 393
321 268
AfeI AGC'GCT
Cuts at: 3107 3107
Size: 9340
AflII C'TTAA_G
Cuts at: 820 1017 2240 820
Size: 197 1223 7920
Fragments arranged by size:
7920 1223 197
AflIII A'CryG_T
Cuts at: 1203 8239 9216 1203
Size: 7036 977 1327
Fragments arranged by size:
7036 1327 977
AgeI A'CCGG_T
Cuts at: 8171 8171
Size: 9340
AhdI GACnn_n'nnGTC
Cuts at: 3733 8069 3733
Size: 4336 5004
AleI CACnn'nnGTG
Cuts at: 1166 4203 5256 6419 1166
Size: 3037 1053 1163 4087
Fragments arranged by size:
4087 3037 1163 1053
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3927 4440 1184
Size: 2743 513 6084
Fragments arranged by size:
6084 2743 513
AlwNI CAG_nnn'CTG
Cuts at: 3538 4336 4874 5416 7083 8655 3538
Size: 798 538 542 1667 1572 4223
Fragments arranged by size:
4223 1667 1572 798 542 538
ApaI G_GGCC'C
Cuts at: 2601 2756 3001 3725 6157 6323 6543 6597 2601
Size: 155 245 724 2432 166 220 54 5344
Fragments arranged by size:
5344 2432 724 245 220 166 155 54
ApaLI G'TGCA_C
Cuts at: 4568 6185 7397 8553 4568
Size: 1617 1212 1156 5355
Fragments arranged by size:
5355 1617 1212 1156
ApoI r'AATT_y
Cuts at: 1493 4718 6649 6875 7027 9136 1493
Size: 3225 1931 226 152 2109 1697
Fragments arranged by size:
3225 2109 1931 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6198 7894 160
Size: 872 5166 1696 1606
Fragments arranged by size:
5166 1696 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9340
AvaI C'yCGr_G
Cuts at: 1870 1943 2385 2610 2755 2946 3150 3345 3684
Size: 73 442 225 145 191 204 195 339
Cuts at: 3684 3767 3777 4151 4251 5006 5548 5619 6348
Size: 83 10 374 100 755 542 71 729
Cuts at: 6348 6544 6665 1870
Size: 196 121 4545
Fragments arranged by size:
4545 755 729 542 442 374 339 225
204 196 195 191 145 121 100 83
73 71 10
BamHI G'GATC_C
Cuts at: 6477 6670 7037 6477
Size: 193 367 8780
Fragments arranged by size:
8780 367 193
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2442 2984 5346 5643 6338
Size: 325 217 627 655 542 2362 297 695
Cuts at: 6338 6661 8116 618
Size: 323 1455 1842
Fragments arranged by size:
2362 1842 1455 695 655 627 542 325
323 297 217
BanII G_rGCy'C
Cuts at: 1391 2535 2601 2756 2826 2922 3001 3048 3336
Size: 1144 66 155 70 96 79 47 288
Cuts at: 3336 3531 3725 4014 5549 6157 6182 6306 6323
Size: 195 194 289 1535 608 25 124 17
Cuts at: 6323 6543 6597 6659 1391
Size: 220 54 62 4072
Fragments arranged by size:
4072 1535 1144 608 289 288 220 195
194 155 124 96 79 70 66 62
54 47 25 17
BbeI G_GCGC'C
Cuts at: 5647 5647
Size: 9340
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2105 2898 3281 4502 4899 928
Size: 971 206 793 383 1221 397 5369
Fragments arranged by size:
5369 1221 971 793 397 383 206
BbvCI CC'TCA_GC
Cuts at: 2271 2271
Size: 9340
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2042 2076 3155 3189 7531 7565 1903
Size: 34 105 34 1079 34 4342 34 3678
Fragments arranged by size:
4342 3678 1079 105 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7232 8442 7232
Size: 1210 8130
BclI T'GATC_A
Cuts at: 1548 4786 1548
Size: 3238 6102
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2959 3492 4352
Size: 107 122 71 631 605 1287 533 860
Cuts at: 4352 7951 136
Size: 3599 1525
Fragments arranged by size:
3599 1525 1287 860 631 605 533 122
107 71
BglII A'GATC_T
Cuts at: 9335 9335
Size: 9340
BlpI GC'TnA_GC
Cuts at: 2921 3047 5585 6295 6757 2921
Size: 126 2538 710 462 5504
Fragments arranged by size:
5504 2538 710 462 126
Bme1580I G_kGCm'C
Cuts at: 1790 2601 2756 3001 3142 3725 4402 4572 5310
Size: 811 155 245 141 583 677 170 738
Cuts at: 5310 5641 6157 6189 6323 6416 6543 6597 7401
Size: 331 516 32 134 93 127 54 804
Cuts at: 7401 8557 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 811 804 738 677 583 516
331 245 170 155 141 134 127 93
54 32
BmgBI CAC'GTC
Cuts at: 1318 4450 5428 1318
Size: 3132 978 5230
Fragments arranged by size:
5230 3132 978
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3138 7071 8029
Size: 416 53 83 399 24 1721 3933 958
Cuts at: 8029 442
Size: 1753
Fragments arranged by size:
3933 1753 1721 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9340
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3271 4159 3271
Size: 888 8452
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 2318 2366 2435 2519 2543 2708 2828
Size: 968 111 48 69 84 24 165 120
Cuts at: 2828 3473 3614 3776 3908 4000 4129 4382 5172
Size: 645 141 162 132 92 129 253 790
Cuts at: 5172 5556 5723 6196 8000 1239
Size: 384 167 473 1804 2579
Fragments arranged by size:
2579 1804 968 790 645 473 384 253
167 165 162 141 132 129 120 111
92 84 69 48 24
Bpu10I CC'TnA_GC
Cuts at: 2271 2271
Size: 9340
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2693 3937 4451 5183 5740 7465 8330 8628 2693
Size: 1244 514 732 557 1725 865 298 3405
Fragments arranged by size:
3405 1725 1244 865 732 557 514 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4130 6294 6554 8003 882
Size: 617 2631 2164 260 1449 2219
Fragments arranged by size:
2631 2219 2164 1449 617 260
BsaAI yAC'GTr
Cuts at: 493 1204 7131 493
Size: 711 5927 2702
Fragments arranged by size:
5927 2702 711
BsaBI GATnn'nnATC
Cuts at: 9334 9334
Size: 9340
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3278 5644 6110 7147
Size: 53 83 186 936 1745 2366 466 1037
Cuts at: 7147 7529 8164 275
Size: 382 635 1451
Fragments arranged by size:
2366 1745 1451 1037 936 635 466 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2868 4014 7773 8171 8445 8592 9003 2868
Size: 1146 3759 398 274 147 411 3205
Fragments arranged by size:
3759 3205 1146 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2316 2346 3005 3035 3251
Size: 30 165 30 913 30 659 30 216
Cuts at: 3251 3281 4001 4031 1178
Size: 30 720 30 6487
Fragments arranged by size:
6487 913 720 659 216 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2663 3026 3215 3275 3515 3911 3914 4112
Size: 1477 363 189 60 240 396 3 198
Cuts at: 4112 4124 4516 5225 5329 6530 6535 1186
Size: 12 392 709 104 1201 5 3991
Fragments arranged by size:
3991 1477 1201 709 396 392 363 240
198 189 104 60 12 5 3
BseYI C'CCAG_C
Cuts at: 2603 2774 3216 3387 3531 3538 3718 3862 5226
Size: 171 442 171 144 7 180 144 1364
Cuts at: 5226 5933 6316 6571 8543 9039 2603
Size: 707 383 255 1972 496 2904
Fragments arranged by size:
2904 1972 1364 707 496 442 383 255
180 171 171 144 144 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3809 4277 4887 5678 6032 6269 6412
Size: 573 2073 468 610 791 354 237 143
Cuts at: 6412 1163
Size: 4091
Fragments arranged by size:
4091 2073 791 610 573 468 354 237
143
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4675 4901 6790 7551 8579 1540
Size: 275 186 2674 226 1889 761 1028 2301
Fragments arranged by size:
2674 2301 1889 1028 761 275 226 186
BsiHKAI G_wGCw'C
Cuts at: 2535 2826 2922 3048 3063 3336 3906 4014 4239
Size: 291 96 126 15 273 570 108 225
Cuts at: 4239 4467 4572 4934 5400 6189 7401 7486 8557
Size: 228 105 362 466 789 1212 85 1071
Cuts at: 8557 2535
Size: 3318
Fragments arranged by size:
3318 1212 1071 789 570 466 362 291
273 228 225 126 108 105 96 85
15
BsmI GAATG_Cn'
Cuts at: 2778 5764 6851 6944 2778
Size: 2986 1087 93 5174
Fragments arranged by size:
5174 2986 1087 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2144 5964 2144
Size: 3820 5520
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2535 2601 2756 2826 2922 3001 3048
Size: 399 745 66 155 70 96 79 47
Cuts at: 3048 3063 3142 3336 3531 3725 3906 4014 4239
Size: 15 79 194 195 194 181 108 225
Cuts at: 4239 4402 4467 4572 4934 5310 5400 5549 5641
Size: 163 65 105 362 376 90 149 92
Cuts at: 5641 6157 6182 6189 6306 6323 6416 6543 6597
Size: 516 25 7 117 17 93 127 54
Cuts at: 6597 6659 7401 7486 8557 1391
Size: 62 742 85 1071 2174
Fragments arranged by size:
2174 1071 745 742 516 399 376 362
225 195 194 194 181 163 155 149
127 117 108 105 96 93 92 90
85 79 79 70 66 65 62 54
47 25 17 15 7
BspEI T'CCGG_A
Cuts at: 2868 9003 2868
Size: 6135 3205
BspHI T'CATG_A
Cuts at: 7229 8959 7229
Size: 1730 7610
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2844 3589 3884 4620 5495 5580 6695 844
Size: 2000 745 295 736 875 85 1115 3489
Fragments arranged by size:
3489 2000 1115 875 745 736 295 85
BsrBI CCG'CTC
Cuts at: 1394 1823 2746 3294 3400 3505 3790 4705 6159
Size: 429 923 548 106 105 285 915 1454
Cuts at: 6159 7227 1394
Size: 1068 3507
Fragments arranged by size:
3507 1454 1068 923 915 548 429 285
106 105
BsrDI GCAATG_nn'
Cuts at: 65 1248 7835 8009 65
Size: 1183 6587 174 1396
Fragments arranged by size:
6587 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2901 3772 4896 6278 7984 8171 1700
Size: 110 1091 871 1124 1382 1706 187 2869
Fragments arranged by size:
2869 1706 1382 1124 1091 871 187 110
BsrGI T'GTAC_A
Cuts at: 96 2394 96
Size: 2298 7042
BssHII G'CGCG_C
Cuts at: 1925 2260 1925
Size: 335 9005
BssSI C'ACGA_G
Cuts at: 2915 3074 3380 5309 7400 8412 2915
Size: 159 306 1929 2091 1012 3843
Fragments arranged by size:
3843 2091 1929 1012 306 159
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5416 6036 794
Size: 4622 620 4098
Fragments arranged by size:
4622 4098 620
BstBI TT'CG_AA
Cuts at: 2179 8144 2179
Size: 5965 3375
BstEII G'GTnAC_C
Cuts at: 1184 4175 1184
Size: 2991 6349
BstXI CCAn_nnnn'nTGG
Cuts at: 2665 4187 5258 5816 5860 6376 2665
Size: 1522 1071 558 44 516 5629
Fragments arranged by size:
5629 1522 1071 558 516 44
BstYI r'GATC_y
Cuts at: 1972 4623 6477 6670 7037 7422 7439 8891 8977
Size: 2651 1854 193 367 385 17 1452 86
Cuts at: 8977 8989 9335 1972
Size: 12 346 1977
Fragments arranged by size:
2651 1977 1854 1452 385 367 346 193
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5034 5975 5034
Size: 941 8399
BtgI C'CryG_G
Cuts at: 513 1065 2004 2580 2701 3327 3856 4290 5373
Size: 552 939 576 121 626 529 434 1083
Cuts at: 5373 6243 6535 6597 513
Size: 870 292 62 3256
Fragments arranged by size:
3256 1083 939 870 626 576 552 529
434 292 121 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3174 487
Size: 683 56 1948 6653
Fragments arranged by size:
6653 1948 683 56
BtsI GCAGTG_nn'
Cuts at: 5398 5414 5690 6856 7650 7670 5398
Size: 16 276 1166 794 20 7068
Fragments arranged by size:
7068 1166 794 276 20 16
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9340
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9305
DraI TTT'AAA
Cuts at: 6644 6644
Size: 9340
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3679 5818 8347 809
Size: 507 2363 2139 2529 1802
Fragments arranged by size:
2529 2363 2139 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2493 4851 4898
Size: 54 1425 50 275 18 663 2358 47
Cuts at: 4898 4935 6787 7676 9305 8
Size: 37 1852 889 1629 43
Fragments arranged by size:
2358 1852 1629 1425 889 663 275 54
50 47 43 37 18
EagI C'GGCC_G
Cuts at: 1537 1812 4898 6787 1537
Size: 275 3086 1889 4090
Fragments arranged by size:
4090 3086 1889 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2004 2844 3492 4046 4485 4980 6224 7270
Size: 651 840 648 554 439 495 1244 1046
Cuts at: 7270 1353
Size: 3423
Fragments arranged by size:
3423 1244 1046 840 651 648 554 495
439
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2816 4199 7927 8301 8447 387
Size: 1064 138 1227 1383 3728 374 146 1280
Fragments arranged by size:
3728 1383 1280 1227 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2533 2824 2920 3046 3334 4012 2533
Size: 291 96 126 288 678 7861
Fragments arranged by size:
7861 678 291 288 126 96
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 2318 2366 2435 2519 2543 2645
Size: 393 575 111 48 69 84 24 102
Cuts at: 2645 2708 2828 3038 3359 3473 3614 3776 3865
Size: 63 120 210 321 114 141 162 89
Cuts at: 3865 3908 4000 4129 4382 4934 5172 5556 5723
Size: 43 92 129 253 552 238 384 167
Cuts at: 5723 5895 6196 7403 8000 8787 9055 1239
Size: 172 301 1207 597 787 268 1524
Fragments arranged by size:
1524 1207 787 597 575 552 393 384
321 301 268 253 238 210 172 167
162 141 129 120 114 111 102 92
89 84 69 63 48 43 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2189 3008 3596 3710 5033 5608 2189
Size: 819 588 114 1323 575 5921
Fragments arranged by size:
5921 1323 819 588 575 114
EcoO109I rG'GnC_Cy
Cuts at: 2329 2598 2752 2997 2998 3233 3479 4545 5140
Size: 269 154 245 1 235 246 1066 595
Cuts at: 5140 5516 5567 5820 5835 6253 6540 6593 2329
Size: 376 51 253 15 418 287 53 5076
Fragments arranged by size:
5076 1066 595 418 376 287 269 253
246 245 235 154 53 51 15 1
EcoRI G'AATT_C
Cuts at: 4718 6649 7027 4718
Size: 1931 378 7031
Fragments arranged by size:
7031 1931 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2428 4564 4738 5078 6448 2428
Size: 2136 174 340 1370 5320
Fragments arranged by size:
5320 2136 1370 340 174
FseI GG_CCGG'CC
Cuts at: 3776 3776
Size: 9340
FspI TGC'GCA
Cuts at: 2483 5480 7846 2483
Size: 2997 2366 3977
Fragments arranged by size:
3977 2997 2366
HaeII r_GCGC'y
Cuts at: 1678 3109 3415 4347 5647 8487 1678
Size: 1431 306 932 1300 2840 2531
Fragments arranged by size:
2840 2531 1431 1300 932 306
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3005 3037 3263 3295 3608
Size: 32 135 32 1433 32 226 32 313
Cuts at: 3608 3640 3719 3751 4001 4033 4252 4284 4862
Size: 32 79 32 250 32 219 32 578
Cuts at: 4862 4894 4916 4948 4949 4981 6194 6226 6551
Size: 32 22 32 1 32 1213 32 325
Cuts at: 6551 6583 7981 8013 8055 8087 1373
Size: 32 1398 32 42 32 2626
Fragments arranged by size:
2626 1433 1398 1213 578 325 313 250
226 219 135 79 42 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 22 1
HincII GTy'rAC
Cuts at: 669 1657 1938 5488 6684 6930 669
Size: 988 281 3550 1196 246 3079
Fragments arranged by size:
3550 3079 1196 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9340
HpaI GTT'AAC
Cuts at: 6930 6930
Size: 9340
KasI G'GCGC_C
Cuts at: 5643 5643
Size: 9340
KpnI G_GTAC'C
Cuts at: 5350 6665 5350
Size: 1315 8025
MfeI C'AATT_G
Cuts at: 6939 6939
Size: 9340
MluI A'CGCG_T
Cuts at: 9216 9216
Size: 9340
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2317 3120 4902 8454 8638 1305
Size: 1012 803 1782 3552 184 2007
Fragments arranged by size:
3552 2007 1782 1012 803 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2495 4853 10
Size: 54 1425 343 663 2358 4497
Fragments arranged by size:
4497 2358 1425 663 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3909 4203 5256 5858 6374 6419 7298
Size: 648 2743 294 1053 602 516 45 879
Cuts at: 7298 7657 7816 518
Size: 359 159 2042
Fragments arranged by size:
2743 2042 1053 879 648 602 516 359
294 159 45
MspA1I CmG'CkG
Cuts at: 1606 2050 3051 3391 3535 3754 4292 4336 4486
Size: 444 1001 340 144 219 538 44 150
Cuts at: 4486 4532 4903 5892 6756 7433 8581 8826 1606
Size: 46 371 989 864 677 1148 245 2120
Fragments arranged by size:
2120 1148 1001 989 864 677 538 444
371 340 245 219 150 144 46 44
NaeI GCC'GGC
Cuts at: 3774 6280 3774
Size: 2506 6834
NarI GG'CG_CC
Cuts at: 5644 5644
Size: 9340
NcoI C'CATG_G
Cuts at: 513 1065 2004 6597 513
Size: 552 939 4593 3256
Fragments arranged by size:
4593 3256 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9340
NgoMIV G'CCGG_C
Cuts at: 3772 6278 3772
Size: 2506 6834
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9340
NotI GC'GGCC_GC
Cuts at: 6787 6787
Size: 9340
NspI r_CATG'y
Cuts at: 1132 1229 2731 4840 6036 6506 6698 8243 1132
Size: 97 1502 2109 1196 470 192 1545 2229
Fragments arranged by size:
2229 2109 1545 1502 1196 470 192 97
PasI CC'CwG_GG
Cuts at: 1348 5674 6630 1348
Size: 4326 956 4058
Fragments arranged by size:
4326 4058 956
PciI A'CATG_T
Cuts at: 8239 8239
Size: 9340
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3538 3862 5933 6571 1108
Size: 2430 324 2071 638 3877
Fragments arranged by size:
3877 2430 2071 638 324
PfoI T'CCnGG_A
Cuts at: 3591 5822 5966 3591
Size: 2231 144 6965
Fragments arranged by size:
6965 2231 144
PmeI GTTT'AAAC
Cuts at: 6644 6644
Size: 9340
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5000 5032 5568 5600 7409 7441 8922 8954 5000
Size: 32 536 32 1809 32 1481 32 5386
Fragments arranged by size:
5386 1809 1481 536 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 5516 5820 5835 6253 5516
Size: 304 15 418 8603
Fragments arranged by size:
8603 418 304 15
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9340
PsiI TTA'TAA
Cuts at: 6910 6910
Size: 9340
PspOMI G'GGCC_C
Cuts at: 2597 2752 2997 3721 6153 6319 6539 6593 2597
Size: 155 245 724 2432 166 220 54 5344
Fragments arranged by size:
5344 2432 724 245 220 166 155 54
PstI C_TGCA'G
Cuts at: 830 2055 2106 2841 3009 3036 3072 3585 4287
Size: 1225 51 735 168 27 36 513 702
Cuts at: 4287 4416 6069 6692 830
Size: 129 1653 623 3478
Fragments arranged by size:
3478 1653 1225 735 702 623 513 168
129 51 36 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9340
PvuII CAG'CTG
Cuts at: 2050 3535 3754 4336 4486 2050
Size: 1485 219 582 150 6904
Fragments arranged by size:
6904 1485 582 219 150
SacI G_AGCT'C
Cuts at: 2535 2826 2922 3048 3336 4014 2535
Size: 291 96 126 288 678 7861
Fragments arranged by size:
7861 678 291 288 126 96
SacII CC_GC'GG
Cuts at: 4293 4293
Size: 9340
SalI G'TCGA_C
Cuts at: 1936 5486 6682 1936
Size: 3550 1196 4594
Fragments arranged by size:
4594 3550 1196
SanDI GG'GwC_CC
Cuts at: 5516 5820 5835 5516
Size: 304 15 9021
Fragments arranged by size:
9021 304 15
SapI GCTCTTCn'nnn_
Cuts at: 4980 4980
Size: 9340
SbfI CC_TGCA'GG
Cuts at: 3009 6692 3009
Size: 3683 5657
ScaI AGT'ACT
Cuts at: 7588 9300 7588
Size: 1712 7628
SfcI C'TryA_G
Cuts at: 826 1045 2051 2102 2837 3005 3032 3068 3581
Size: 219 1006 51 735 168 27 36 513
Cuts at: 3581 4283 4412 6065 6688 7823 8504 8695 826
Size: 702 129 1653 623 1135 681 191 1471
Fragments arranged by size:
1653 1471 1135 1006 735 702 681 623
513 219 191 168 129 51 36 27
SfoI GGC'GCC
Cuts at: 5645 5645
Size: 9340
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9340
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9340
SmaI CCC'GGG
Cuts at: 1872 2612 2757 2948 3152 3347 3686 4253 5550
Size: 740 145 191 204 195 339 567 1297
Cuts at: 5550 5621 6546 6667 1872
Size: 71 925 121 4545
Fragments arranged by size:
4545 1297 925 740 567 339 204 195
191 145 121 71
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2240 2708 3916 4466 5198 5719
Size: 197 926 297 468 1208 550 732 521
Cuts at: 5719 7444 8345 8607 820
Size: 1725 901 262 1553
Fragments arranged by size:
1725 1553 1208 926 901 732 550 521
468 297 262 197
SnaBI TAC'GTA
Cuts at: 493 7131 493
Size: 6638 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9340
SphI G_CATG'C
Cuts at: 1132 4840 6698 1132
Size: 3708 1858 3774
Fragments arranged by size:
3774 3708 1858
SspI AAT'ATT
Cuts at: 5 52 7264 5
Size: 47 7212 2081
Fragments arranged by size:
7212 2081 47
StuI AGG'CCT
Cuts at: 1517 3587 4355 1517
Size: 2070 768 6502
Fragments arranged by size:
6502 2070 768
StyI C'CwwG_G
Cuts at: 513 1065 2004 5559 6597 6779 513
Size: 552 939 3555 1038 182 3074
Fragments arranged by size:
3555 3074 1038 939 552 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2788 4224 5254 5303 6276 7367 7384
Size: 1255 1208 1436 1030 49 973 1091 17
Cuts at: 7384 7537 7722 8167 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1436 1255 1208 1091 1030 973 445
185 153 49 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2394 2541 5202
Size: 274 80 33 51 1443 417 147 2661
Cuts at: 5202 7586 9298 96
Size: 2384 1712 138
Fragments arranged by size:
2661 2384 1712 1443 417 274 147 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4439 4471 4709 4741 9300 9332 4439
Size: 32 238 32 4559 32 4447
Fragments arranged by size:
4559 4447 238 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9340
XbaI T'CTAG_A
Cuts at: 6676 6676
Size: 9340
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3703 4854 3703
Size: 1151 8189
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9340
XmaI C'CCGG_G
Cuts at: 1870 2610 2755 2946 3150 3345 3684 4251 5548
Size: 740 145 191 204 195 339 567 1297
Cuts at: 5548 5619 6544 6665 1870
Size: 71 925 121 4545
Fragments arranged by size:
4545 1297 925 740 567 339 204 195
191 145 121 71
XmnI GAAnn'nnTTC
Cuts at: 3566 4034 4718 7469 3566
Size: 468 684 2751 5437
Fragments arranged by size:
5437 2751 684 468
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3279 6111 7148 276
Size: 53 83 186 936 1745 2832 1037 2468
Fragments arranged by size:
2832 2468 1745 1037 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII
EcoRV FspAI NruI NsiI PacI PmlI PspXI PsrI
RsrII SexAI SfiI SrfI SwaI