Restriction map for
pFN21AA0799
(Circular) (Six-base) MAPSORT of: pFN21AA0799.seq Check: 7039 from: 1 to: 10879
pFN21AA0799 10879 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4651 4651
Size: 10879
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4101 8689 278
Size: 53 83 186 936 2565 4588 2468
Fragments arranged by size:
4588 2565 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3001 3189 7257 7950 8222 1937
Size: 1064 188 4068 693 272 4594
Fragments arranged by size:
4594 4068 1064 693 272 188
Acc65I G'GTAC_C
Cuts at: 8200 8200
Size: 10879
AclI AA'CG_TT
Cuts at: 1563 9006 9379 1563
Size: 7443 373 3063
Fragments arranged by size:
7443 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3362 4939 5076 6266 6683 8942 10326 10594
Size: 1730 1577 137 1190 417 2259 1384 268
Cuts at: 10594 1632
Size: 1917
Fragments arranged by size:
2259 1917 1730 1577 1384 1190 417 268
137
AfeI AGC'GCT
Cuts at: 5714 8135 5714
Size: 2421 8458
AflII C'TTAA_G
Cuts at: 820 1017 7031 820
Size: 197 6014 4668
Fragments arranged by size:
6014 4668 197
AflIII A'CryG_T
Cuts at: 1203 3772 5931 7224 7734 9778 10755 1203
Size: 2569 2159 1293 510 2044 977 1327
Fragments arranged by size:
2569 2159 2044 1327 1293 977 510
AgeI A'CCGG_T
Cuts at: 5406 9710 5406
Size: 4304 6575
AhdI GACnn_n'nnGTC
Cuts at: 2209 3286 9608 2209
Size: 1077 6322 3480
Fragments arranged by size:
6322 3480 1077
AleI CACnn'nnGTG
Cuts at: 1166 5123 6164 1166
Size: 3957 1041 5881
Fragments arranged by size:
5881 3957 1041
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4136 5174 6938 1184
Size: 2952 1038 1764 5125
Fragments arranged by size:
5125 2952 1764 1038
AlwNI CAG_nnn'CTG
Cuts at: 4204 4729 4759 6345 6529 6739 6898 7516 7729
Size: 525 30 1586 184 210 159 618 213
Cuts at: 7729 8622 10194 4204
Size: 893 1572 4889
Fragments arranged by size:
4889 1586 1572 893 618 525 213 210
184 159 30
ApaI G_GGCC'C
Cuts at: 4258 4258
Size: 10879
ApaLI G'TGCA_C
Cuts at: 4111 8936 10092 4111
Size: 4825 1156 4898
Fragments arranged by size:
4898 4825 1156
ApoI r'AATT_y
Cuts at: 1493 2077 2834 3023 4901 6179 7352 7793 8188
Size: 584 757 189 1878 1278 1173 441 395
Cuts at: 8188 8414 8566 10675 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1878 1697 1278 1173 757 584 441
395 226 189 152
AseI AT'TA_AT
Cuts at: 160 1032 9433 160
Size: 872 8401 1606
Fragments arranged by size:
8401 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10879
AvaI C'yCGr_G
Cuts at: 1870 1943 2768 4829 5395 7065 7764 8204 1870
Size: 73 825 2061 566 1670 699 440 4545
Fragments arranged by size:
4545 2061 1670 825 699 566 440 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 7496 7529 7496
Size: 33 10846
BamHI G'GATC_C
Cuts at: 7691 8209 8576 7691
Size: 518 367 9994
Fragments arranged by size:
9994 518 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2373 5125 5300 5399 5780
Size: 325 217 627 586 2752 175 99 381
Cuts at: 5780 5996 8014 8200 9655 618
Size: 216 2018 186 1455 1842
Fragments arranged by size:
2752 2018 1842 1455 627 586 381 325
217 216 186 175 99
BanII G_rGCy'C
Cuts at: 1391 2226 4258 4548 4785 5681 8198 1391
Size: 835 2032 290 237 896 2517 4072
Fragments arranged by size:
4072 2517 2032 896 835 290 237
BbeI G_GCGC'C
Cuts at: 2377 2377
Size: 10879
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2095 2576 2642 2898 4039 4172 6225
Size: 971 196 481 66 256 1141 133 2053
Cuts at: 6225 7975 928
Size: 1750 3832
Fragments arranged by size:
3832 2053 1750 1141 971 481 256 196
133 66
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4092 4105 4126 4139 9070 9104 1903
Size: 34 2155 13 21 13 4931 34 3678
Fragments arranged by size:
4931 3678 2155 34 34 21 13 13
BciVI GTATCCnnnnn_n'
Cuts at: 3790 6963 8771 9981 3790
Size: 3173 1808 1210 4688
Fragments arranged by size:
4688 3173 1808 1210
BclI T'GATC_A
Cuts at: 1548 6066 7842 1548
Size: 4518 1776 4585
Fragments arranged by size:
4585 4518 1776
BfrBI ATG'CAT
Cuts at: 5962 5962
Size: 10879
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2382 7128 9490
Size: 107 122 71 631 605 710 4746 2362
Cuts at: 9490 136
Size: 1525
Fragments arranged by size:
4746 2362 1525 710 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3074 10874 3074
Size: 7800 3079
BlpI GC'TnA_GC
Cuts at: 3881 8296 3881
Size: 4415 6464
Bme1580I G_kGCm'C
Cuts at: 1790 3046 4115 4258 5130 5783 5999 6858 8017
Size: 1256 1069 143 872 653 216 859 1159
Cuts at: 8017 8940 10096 1790
Size: 923 1156 2573
Fragments arranged by size:
2573 1256 1159 1156 1069 923 872 859
653 216 143
BmgBI CAC'GTC
Cuts at: 1318 2947 7255 1318
Size: 1629 4308 4942
Fragments arranged by size:
4942 4308 1629
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5133 6329 8610
Size: 416 53 83 399 24 3716 1196 2281
Cuts at: 8610 9568 442
Size: 958 1753
Fragments arranged by size:
3716 2281 1753 1196 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10879
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6956 6956
Size: 10879
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3675 4013 4226 4658 4703 4814 5188 6761
Size: 2436 338 213 432 45 111 374 1573
Cuts at: 6761 7469 7493 7655 8158 9539 1239
Size: 708 24 162 503 1381 2579
Fragments arranged by size:
2579 2436 1573 1381 708 503 432 374
338 213 162 111 45 24
Bpu10I CC'TnA_GC
Cuts at: 6908 6908
Size: 10879
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2501 3528 5585 5630 5759 6926 6935 7568 9004
Size: 1027 2057 45 129 1167 9 633 1436
Cuts at: 9004 9869 10167 2501
Size: 865 298 3213
Fragments arranged by size:
3213 2057 1436 1167 1027 865 633 298
129 45 9
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 9542 882
Size: 617 8043 2219
Fragments arranged by size:
8043 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 8031 8670 493
Size: 711 6827 639 2702
Fragments arranged by size:
6827 2702 711 639
BsaBI GATnn'nnATC
Cuts at: 5843 7148 10873 5843
Size: 1305 3725 5849
Fragments arranged by size:
5849 3725 1305
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2210 2374 4098 5010
Size: 53 83 186 936 677 164 1724 912
Cuts at: 5010 7938 8686 9068 9703 275
Size: 2928 748 382 635 1451
Fragments arranged by size:
2928 1724 1451 936 912 748 677 635
382 186 164 83 53
BsaWI w'CCGG_w
Cuts at: 2106 4866 5073 5406 7683 9312 9710 9984 10131
Size: 2760 207 333 2277 1629 398 274 147
Cuts at: 10131 10542 2106
Size: 411 2443
Fragments arranged by size:
2760 2443 2277 1629 411 398 333 274
207 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2279 2309 3492 3522 4790
Size: 30 165 30 876 30 1183 30 1268
Cuts at: 4790 4820 5186 5216 5237 5267 6734 6764 7928
Size: 30 366 30 21 30 1467 30 1164
Cuts at: 7928 7958 1178
Size: 30 4099
Fragments arranged by size:
4099 1467 1268 1183 1164 876 366 165
30 30 30 30 30 30 30 30
30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2204 3877 4363 4366 4811 4949 4988 4991
Size: 1018 1673 486 3 445 138 39 3
Cuts at: 4991 5111 5191 5671 5780 6128 7730 7733 7747
Size: 120 80 480 109 348 1602 3 14
Cuts at: 7747 1186
Size: 4318
Fragments arranged by size:
4318 1673 1602 1018 486 480 445 348
138 120 109 80 39 14 3 3
3
BseYI C'CCAG_C
Cuts at: 4016 4159 4554 5033 5240 6522 7169 10082 10578
Size: 143 395 479 207 1282 647 2913 496
Cuts at: 10578 4016
Size: 4317
Fragments arranged by size:
4317 2913 1282 647 496 479 395 207
143
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2106 4828 5195 6074 6898 7520 1163
Size: 573 370 2722 367 879 824 622 4522
Fragments arranged by size:
4522 2722 879 824 622 573 370 367
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6510 8329 9090 10118 1540
Size: 275 186 4509 1819 761 1028 2301
Fragments arranged by size:
4509 2301 1819 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2147 2226 4115 4548 4785 5043 8940 9025 10096
Size: 79 1889 433 237 258 3897 85 1071
Cuts at: 10096 2147
Size: 2930
Fragments arranged by size:
3897 2930 1889 1071 433 258 237 85
79
BsmI GAATG_Cn'
Cuts at: 3482 3862 4337 5992 8390 8483 3482
Size: 380 475 1655 2398 93 5878
Fragments arranged by size:
5878 2398 1655 475 380 93
BsmBI CGTCTCn'nnnn_
Cuts at: 7948 7948
Size: 10879
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2147 2226 3046 4115 4258 4548 4785
Size: 399 357 79 820 1069 143 290 237
Cuts at: 4785 5043 5130 5681 5783 5999 6858 8017 8198
Size: 258 87 551 102 216 859 1159 181
Cuts at: 8198 8940 9025 10096 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1159 1071 1069 859 820 742 551
399 357 290 258 237 216 181 143
102 87 85 79
BspEI T'CCGG_A
Cuts at: 2106 4866 5073 7683 10542 2106
Size: 2760 207 2610 2859 2443
Fragments arranged by size:
2859 2760 2610 2443 207
BspHI T'CATG_A
Cuts at: 8100 8768 10498 8100
Size: 668 1730 8481
Fragments arranged by size:
8481 1730 668
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2477 4651 4710 6807 7350 8234 844
Size: 1633 2174 59 2097 543 884 3489
Fragments arranged by size:
3489 2174 2097 1633 884 543 59
BsrBI CCG'CTC
Cuts at: 1394 1823 4768 4879 5628 6634 8766 1394
Size: 429 2945 111 749 1006 2132 3507
Fragments arranged by size:
3507 2945 2132 1006 749 429 111
BsrDI GCAATG_nn'
Cuts at: 65 1248 2635 2946 3280 9374 9548 65
Size: 1183 1387 311 334 6094 174 1396
Fragments arranged by size:
6094 1396 1387 1183 334 311 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2370 5406 5496 6429 9523 9710 1700
Size: 110 560 3036 90 933 3094 187 2869
Fragments arranged by size:
3094 3036 2869 933 560 187 110 90
BsrGI T'GTAC_A
Cuts at: 96 2643 5511 5550 6882 96
Size: 2547 2868 39 1332 4093
Fragments arranged by size:
4093 2868 2547 1332 39
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10879
BssSI C'ACGA_G
Cuts at: 4114 8939 9951 4114
Size: 4825 1012 5042
Fragments arranged by size:
5042 4825 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3934 4759 6799 794
Size: 3140 825 2040 4874
Fragments arranged by size:
4874 3140 2040 825
BstBI TT'CG_AA
Cuts at: 4995 9683 4995
Size: 4688 6191
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 10879
BstXI CCAn_nnnn'nTGG
Cuts at: 4915 5240 7834 8080 4915
Size: 325 2594 246 7714
Fragments arranged by size:
7714 2594 325 246
BstYI r'GATC_y
Cuts at: 1972 3074 3156 4671 5949 6894 7691 8209 8576
Size: 1102 82 1515 1278 945 797 518 367
Cuts at: 8576 8961 8978 10430 10516 10528 10874 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1515 1452 1278 1102 945 797 518
385 367 346 86 82 17 12
BstZ17I GTA'TAC
Cuts at: 3002 3002
Size: 10879
Bsu36I CC'TnA_GG
Cuts at: 3143 4092 7670 7908 3143
Size: 949 3578 238 6114
Fragments arranged by size:
6114 3578 949 238
BtgI C'CryG_G
Cuts at: 513 1065 2004 2227 2355 3210 4242 5937 7110
Size: 552 939 223 128 855 1032 1695 1173
Cuts at: 7110 7123 7206 7346 7453 7695 7821 513
Size: 13 83 140 107 242 126 3571
Fragments arranged by size:
3571 1695 1173 1032 939 855 552 242
223 140 128 126 107 83 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2314 2395 5097 487
Size: 683 56 1088 81 2702 6269
Fragments arranged by size:
6269 2702 1088 683 81 56
BtsI GCAGTG_nn'
Cuts at: 2798 3030 3857 6091 6874 8395 9189 9209 2798
Size: 232 827 2234 783 1521 794 20 4468
Fragments arranged by size:
4468 2234 1521 827 794 783 232 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10879
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 7879 7914 529
Size: 35 7315 35 3494
Fragments arranged by size:
7315 3494 35 35
DraI TTT'AAA
Cuts at: 8183 8183
Size: 10879
DraIII CAC_nnn'GTG
Cuts at: 6465 6465
Size: 10879
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4862 9886 809
Size: 507 3546 5024 1802
Fragments arranged by size:
5024 3546 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2053 3207 4239
Size: 54 1425 50 275 18 223 1154 1032
Cuts at: 4239 5044 5853 6075 7203 7249 7456 7824 8091
Size: 805 809 222 1128 46 207 368 267
Cuts at: 8091 8326 9215 10844 8
Size: 235 889 1629 43
Fragments arranged by size:
1629 1425 1154 1128 1032 889 809 805
368 275 267 235 223 222 207 54
50 46 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 8326 1537
Size: 275 6514 4090
Fragments arranged by size:
6514 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3147 4215 4473 4528 4598 4662 5095 5445
Size: 1794 1068 258 55 70 64 433 350
Cuts at: 5445 7019 7554 7634 8809 1353
Size: 1574 535 80 1175 3423
Fragments arranged by size:
3423 1794 1574 1175 1068 535 433 350
258 80 70 64 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5717 7660 7690 8143 9466 9840
Size: 1064 138 4128 1943 30 453 1323 374
Cuts at: 9840 9986 387
Size: 146 1280
Fragments arranged by size:
4128 1943 1323 1280 1064 453 374 146
138 30
EcoICRI GAG'CTC
Cuts at: 2224 4546 4783 2224
Size: 2322 237 8320
Fragments arranged by size:
8320 2322 237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3362 3675 4013 4226 4658 4703 4814
Size: 393 1730 313 338 213 432 45 111
Cuts at: 4814 4939 5076 5188 6266 6683 6761 7469 7493
Size: 125 137 112 1078 417 78 708 24
Cuts at: 7493 7655 8158 8942 9539 10326 10594 1239
Size: 162 503 784 597 787 268 1524
Fragments arranged by size:
1730 1524 1078 787 784 708 597 503
432 417 393 338 313 268 213 162
137 125 112 111 78 45 24
EcoNI CCTnn'n_nnAGG
Cuts at: 4370 4370
Size: 10879
EcoO109I rG'GnC_Cy
Cuts at: 4297 4699 5101 5382 5641 6324 6502 7339 7665
Size: 402 402 281 259 683 178 837 326
Cuts at: 7665 4297
Size: 7511
Fragments arranged by size:
7511 837 683 402 402 326 281 259
178
EcoRI G'AATT_C
Cuts at: 6179 8188 8566 6179
Size: 2009 378 8492
Fragments arranged by size:
8492 2009 378
EcoRV GAT'ATC
Cuts at: 6541 6541
Size: 10879
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4963 4963
Size: 10879
FspI TGC'GCA
Cuts at: 9385 9385
Size: 10879
HaeII r_GCGC'y
Cuts at: 1678 2377 2446 5716 8137 10026 1678
Size: 699 69 3270 2421 1889 2531
Fragments arranged by size:
3270 2531 2421 1889 699 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2195 2227 2558 2590 2900
Size: 32 135 32 623 32 331 32 310
Cuts at: 2900 2932 3141 3173 3272 3304 4063 4095 4663
Size: 32 209 32 99 32 759 32 568
Cuts at: 4663 4695 5237 5269 6734 6766 7683 7715 7758
Size: 32 542 32 1465 32 917 32 43
Cuts at: 7758 7790 7928 7960 9520 9552 9594 9626 1373
Size: 32 138 32 1560 32 42 32 2626
Fragments arranged by size:
2626 1560 1465 917 759 623 568 542
331 310 209 138 135 99 43 42
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2540 3628 7258 7605 8223 8469
Size: 988 281 602 1088 3630 347 618 246
Cuts at: 8469 669
Size: 3079
Fragments arranged by size:
3630 3079 1088 988 618 602 347 281
246
HindIII A'AGCT_T
Cuts at: 748 2619 748
Size: 1871 9008
HpaI GTT'AAC
Cuts at: 3628 8469 3628
Size: 4841 6038
KasI G'GCGC_C
Cuts at: 2373 2373
Size: 10879
KpnI G_GTAC'C
Cuts at: 8204 8204
Size: 10879
MfeI C'AATT_G
Cuts at: 4433 8478 4433
Size: 4045 6834
MluI A'CGCG_T
Cuts at: 5931 10755 5931
Size: 4824 6055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4167 5358 9993 10177 1305
Size: 2862 1191 4635 184 2007
Fragments arranged by size:
4635 2862 2007 1191 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2055 3209 7205 7458 7826
Size: 54 1425 343 223 1154 3996 253 368
Cuts at: 7826 8093 10
Size: 267 2796
Fragments arranged by size:
3996 2796 1425 1154 368 343 267 253
223 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3603 5123 5849 6164 8117 8123 8837
Size: 648 2437 1520 726 315 1953 6 714
Cuts at: 8837 9196 9355 518
Size: 359 159 2042
Fragments arranged by size:
2437 2042 1953 1520 726 714 648 359
315 159 6
MspA1I CmG'CkG
Cuts at: 1606 2260 2430 2683 3973 4159 5208 5370 5517
Size: 654 170 253 1290 186 1049 162 147
Cuts at: 5517 5589 6529 6888 7169 8295 8972 10120 10365
Size: 72 940 359 281 1126 677 1148 245
Cuts at: 10365 1606
Size: 2120
Fragments arranged by size:
2120 1290 1148 1126 1049 940 677 654
359 281 253 245 186 170 162 147
72
NaeI GCC'GGC
Cuts at: 2372 2372
Size: 10879
NarI GG'CG_CC
Cuts at: 2374 2374
Size: 10879
NcoI C'CATG_G
Cuts at: 513 1065 2004 2227 2355 3210 4242 7123 7206
Size: 552 939 223 128 855 1032 2881 83
Cuts at: 7206 7346 7453 7821 513
Size: 140 107 368 3571
Fragments arranged by size:
3571 2881 1032 939 855 552 368 223
140 128 107 83
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10879
NgoMIV G'CCGG_C
Cuts at: 2370 2370
Size: 10879
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10879
NotI GC'GGCC_GC
Cuts at: 8326 8326
Size: 10879
NsiI A_TGCA'T
Cuts at: 5964 5964
Size: 10879
NspI r_CATG'y
Cuts at: 1132 1229 3590 3776 3928 4932 5230 6906 7228
Size: 97 2361 186 152 1004 298 1676 322
Cuts at: 7228 7738 8237 9782 1132
Size: 510 499 1545 2229
Fragments arranged by size:
2361 2229 1676 1545 1004 510 499 322
298 186 152 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 10879
PciI A'CATG_T
Cuts at: 3772 7224 7734 9778 3772
Size: 3452 510 2044 4873
Fragments arranged by size:
4873 3452 2044 510
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4264 5904 6465 1108
Size: 3156 1640 561 5522
Fragments arranged by size:
5522 3156 1640 561
PfoI T'CCnGG_A
Cuts at: 5183 5693 5952 6985 7377 5183
Size: 510 259 1033 392 8685
Fragments arranged by size:
8685 1033 510 392 259
PmeI GTTT'AAAC
Cuts at: 8183 8183
Size: 10879
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7986 8018 8948 8980 10461 10493 7986
Size: 32 930 32 1481 32 8372
Fragments arranged by size:
8372 1481 930 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4297 4699 5101 5382 6324 7339 7665 4297
Size: 402 402 281 942 1015 326 7511
Fragments arranged by size:
7511 1015 942 402 402 326 281
PshAI GACnn'nnGTC
Cuts at: 1613 2118 4172 6329 1613
Size: 505 2054 2157 6163
Fragments arranged by size:
6163 2157 2054 505
PsiI TTA'TAA
Cuts at: 8449 8449
Size: 10879
PspOMI G'GGCC_C
Cuts at: 4254 4254
Size: 10879
PspXI vC'TCGA_Gb
Cuts at: 4829 7764 4829
Size: 2935 7944
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 6938 6970 6938
Size: 32 10847
PstI C_TGCA'G
Cuts at: 830 4128 4707 4755 4764 6144 6735 7521 7578
Size: 3298 579 48 9 1380 591 786 57
Cuts at: 7578 8231 830
Size: 653 3478
Fragments arranged by size:
3478 3298 1380 786 653 591 579 57
48 9
PvuI CG_AT'CG
Cuts at: 2001 6510 2001
Size: 4509 6370
PvuII CAG'CTG
Cuts at: 2683 3973 4159 5517 6529 6888 2683
Size: 1290 186 1358 1012 359 6674
Fragments arranged by size:
6674 1358 1290 1012 359 186
RsrII CG'GwC_CG
Cuts at: 4027 4027
Size: 10879
SacI G_AGCT'C
Cuts at: 2226 4548 4785 2226
Size: 2322 237 8320
Fragments arranged by size:
8320 2322 237
SalI G'TCGA_C
Cuts at: 1936 7256 8221 1936
Size: 5320 965 4594
Fragments arranged by size:
5320 4594 965
SanDI GG'GwC_CC
Cuts at: 6324 7665 6324
Size: 1341 9538
SapI GCTCTTCn'nnn_
Cuts at: 4598 4598
Size: 10879
SbfI CC_TGCA'GG
Cuts at: 8231 8231
Size: 10879
ScaI AGT'ACT
Cuts at: 2071 3386 9127 10839 2071
Size: 1315 5741 1712 2111
Fragments arranged by size:
5741 2111 1712 1315
SfcI C'TryA_G
Cuts at: 826 1045 3831 4124 4703 4751 4760 6140 6731
Size: 219 2786 293 579 48 9 1380 591
Cuts at: 6731 6782 7517 7574 8137 8227 9362 10043 10234
Size: 51 735 57 563 90 1135 681 191
Cuts at: 10234 826
Size: 1471
Fragments arranged by size:
2786 1471 1380 1135 735 681 591 579
563 293 219 191 90 57 51 48
9
SfoI GGC'GCC
Cuts at: 2375 2375
Size: 10879
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10879
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10879
SmaI CCC'GGG
Cuts at: 1872 2770 8206 1872
Size: 898 5436 4545
Fragments arranged by size:
5436 4545 898
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2516 3543 4829 5600 5645 5774
Size: 197 926 573 1027 1286 771 45 129
Cuts at: 5774 6941 6950 7031 7583 7764 8983 9884 10146
Size: 1167 9 81 552 181 1219 901 262
Cuts at: 10146 820
Size: 1553
Fragments arranged by size:
1553 1286 1219 1167 1027 926 901 771
573 552 262 197 181 129 81 45
9
SnaBI TAC'GTA
Cuts at: 493 8031 8670 493
Size: 7538 639 2702
Fragments arranged by size:
7538 2702 639
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10879
SphI G_CATG'C
Cuts at: 1132 3590 4932 5230 8237 1132
Size: 2458 1342 298 3007 3774
Fragments arranged by size:
3774 3007 2458 1342 298
SspI AAT'ATT
Cuts at: 5 52 2707 8803 5
Size: 47 2655 6096 2081
Fragments arranged by size:
6096 2655 2081 47
StuI AGG'CCT
Cuts at: 1517 7200 1517
Size: 5683 5196
StyI C'CwwG_G
Cuts at: 513 1065 2004 2227 2355 3210 4170 4191 4242
Size: 552 939 223 128 855 960 21 51
Cuts at: 4242 5826 6000 6498 7123 7206 7346 7453 7821
Size: 1584 174 498 625 83 140 107 368
Cuts at: 7821 8318 513
Size: 497 3074
Fragments arranged by size:
3074 1584 960 939 855 625 552 498
497 368 223 174 140 128 107 83
51 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4754 6284 6536 6626 7913 8906 8923
Size: 1255 3174 1530 252 90 1287 993 17
Cuts at: 8923 9076 9261 9706 325
Size: 153 185 445 1498
Fragments arranged by size:
3174 1530 1498 1287 1255 993 445 252
185 153 90 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2069 2364 2643
Size: 274 80 33 51 1443 92 295 279
Cuts at: 2643 3384 5511 5550 5745 6262 6822 6882 7830
Size: 741 2127 39 195 517 560 60 948
Cuts at: 7830 9125 10837 96
Size: 1295 1712 138
Fragments arranged by size:
2127 1712 1443 1295 948 741 560 517
295 279 274 195 138 92 80 60
51 39 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4790 4822 5186 5218 10839 10871 4790
Size: 32 364 32 5621 32 4798
Fragments arranged by size:
5621 4798 364 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 7940 1316
Size: 6624 4255
XbaI T'CTAG_A
Cuts at: 8215 8215
Size: 10879
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5063 7166 8080 5063
Size: 2103 914 7862
Fragments arranged by size:
7862 2103 914
XhoI C'TCGA_G
Cuts at: 1943 4829 7764 1943
Size: 2886 2935 5058
Fragments arranged by size:
5058 2935 2886
XmaI C'CCGG_G
Cuts at: 1870 2768 8204 1870
Size: 898 5436 4545
Fragments arranged by size:
5436 4545 898
XmnI GAAnn'nnTTC
Cuts at: 6374 6981 9008 6374
Size: 607 2027 8245
Fragments arranged by size:
8245 2027 607
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4099 8687 276
Size: 53 83 186 936 2565 4588 2468
Fragments arranged by size:
4588 2565 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbvCI BsiWI FseI FspAI NruI
PacI PmlI SacII SexAI SfiI SrfI SwaI