Restriction map for
pFN21AA0790
(Circular) (Six-base) MAPSORT of: pFN21AA0790.seq Check: 5114 from: 1 to: 8446
pFN21AA0790 8446 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5427 6256 278
Size: 53 83 186 936 3891 829 2468
Fragments arranged by size:
3891 2468 936 829 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4668 5789 1937
Size: 2731 1121 4594
Fragments arranged by size:
4594 2731 1121
Acc65I G'GTAC_C
Cuts at: 5767 5767
Size: 8446
AclI AA'CG_TT
Cuts at: 1563 2708 6573 6946 1563
Size: 1145 3865 373 3063
Fragments arranged by size:
3865 3063 1145 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2303 2883 3049 4358 4623 5334 5394 5596
Size: 671 580 166 1309 265 711 60 202
Cuts at: 5596 5639 6509 7893 8161 1632
Size: 43 870 1384 268 1917
Fragments arranged by size:
1917 1384 1309 870 711 671 580 268
265 202 166 60 43
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8249
AflIII A'CryG_T
Cuts at: 1203 2232 2880 4386 7345 8322 1203
Size: 1029 648 1506 2959 977 1327
Fragments arranged by size:
2959 1506 1327 1029 977 648
AgeI A'CCGG_T
Cuts at: 7277 7277
Size: 8446
AhdI GACnn_n'nnGTC
Cuts at: 7175 7175
Size: 8446
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8446
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2217 2682 4677 1184
Size: 1033 465 1995 4953
Fragments arranged by size:
4953 1995 1033 465
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4873 4905 4873
Size: 32 8414
AlwNI CAG_nnn'CTG
Cuts at: 2281 4153 4648 4757 5735 6189 7761 2281
Size: 1872 495 109 978 454 1572 2966
Fragments arranged by size:
2966 1872 1572 978 495 454 109
ApaI G_GGCC'C
Cuts at: 4487 5024 5035 5240 4487
Size: 537 11 205 7693
Fragments arranged by size:
7693 537 205 11
ApaLI G'TGCA_C
Cuts at: 2933 3686 6503 7659 2933
Size: 753 2817 1156 3720
Fragments arranged by size:
3720 2817 1156 753
ApoI r'AATT_y
Cuts at: 1493 2890 4941 5755 5981 6133 8242 1493
Size: 1397 2051 814 226 152 2109 1697
Fragments arranged by size:
2109 2051 1697 1397 814 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7000 160
Size: 872 5968 1606
Fragments arranged by size:
5968 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8446
AvaI C'yCGr_G
Cuts at: 1870 1943 2029 2048 2054 2066 3194 3436 4170
Size: 73 86 19 6 12 1128 242 734
Cuts at: 4170 5198 5268 5414 5771 1870
Size: 1028 70 146 357 4545
Fragments arranged by size:
4545 1128 1028 734 357 242 146 86
73 70 19 12 6
BamHI G'GATC_C
Cuts at: 4118 5776 6143 4118
Size: 1658 367 6421
Fragments arranged by size:
6421 1658 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4898 5146 5591 5767 7222
Size: 325 217 627 3111 248 445 176 1455
Cuts at: 7222 618
Size: 1842
Fragments arranged by size:
3111 1842 1455 627 445 325 248 217
176
BanII G_rGCy'C
Cuts at: 1391 2049 2055 2067 2073 2097 3563 4263 4487
Size: 658 6 12 6 24 1466 700 224
Cuts at: 4487 4745 5024 5035 5196 5240 5765 1391
Size: 258 279 11 161 44 525 4072
Fragments arranged by size:
4072 1466 700 658 525 279 258 224
161 44 24 12 11 6 6
BbeI G_GCGC'C
Cuts at: 5150 5150
Size: 8446
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2424 2529 3527 3855 4023 4622 928
Size: 971 525 105 998 328 168 599 4752
Fragments arranged by size:
4752 998 971 599 525 328 168 105
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6637 6671 1903
Size: 34 4700 34 3678
Fragments arranged by size:
4700 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2165 6338 7548 2165
Size: 4173 1210 3063
Fragments arranged by size:
4173 3063 1210
BclI T'GATC_A
Cuts at: 1548 2604 1548
Size: 1056 7390
BfrBI ATG'CAT
Cuts at: 4676 4676
Size: 8446
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5182 7057 136
Size: 107 122 71 631 605 3510 1875 1525
Fragments arranged by size:
3510 1875 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8441 8441
Size: 8446
BlpI GC'TnA_GC
Cuts at: 4551 4806 4920 5863 4551
Size: 255 114 943 7134
Fragments arranged by size:
7134 943 255 114
Bme1580I G_kGCm'C
Cuts at: 1790 2937 3690 4487 4901 5024 5035 5240 5269
Size: 1147 753 797 414 123 11 205 29
Cuts at: 5269 6507 7663 1790
Size: 1238 1156 2573
Fragments arranged by size:
2573 1238 1156 1147 797 753 414 205
123 29 11
BmgBI CAC'GTC
Cuts at: 1318 2146 2233 2313 3006 3838 4389 4561 1318
Size: 828 87 80 693 832 551 172 5203
Fragments arranged by size:
5203 832 828 693 551 172 87 80
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2180 3699 4442
Size: 416 53 83 399 24 763 1519 743
Cuts at: 4442 5019 5034 6177 7135 442
Size: 577 15 1143 958 1753
Fragments arranged by size:
1753 1519 1143 958 763 743 577 416
399 83 53 24 15
BmtI G_CTAG'C
Cuts at: 1056 2307 4233 1056
Size: 1251 1926 5269
Fragments arranged by size:
5269 1926 1251
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2143 3451 4079 4675 2143
Size: 1308 628 596 5914
Fragments arranged by size:
5914 1308 628 596
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3001 4028 4499 4670 4757 4836 4868 5198
Size: 1762 1027 471 171 87 79 32 330
Cuts at: 5198 7106 1239
Size: 1908 2579
Fragments arranged by size:
2579 1908 1762 1027 471 330 171 87
79 32
Bpu10I CC'TnA_GC
Cuts at: 2043 3203 2043
Size: 1160 7286
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2618 3494 3713 3719 6571 7436 7734 2618
Size: 876 219 6 2852 865 298 3330
Fragments arranged by size:
3330 2852 876 865 298 219 6
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3197 4466 5263 7109 882
Size: 617 1698 1269 797 1846 2219
Fragments arranged by size:
2219 1846 1698 1269 797 617
BsaAI yAC'GTr
Cuts at: 493 1204 2881 3832 6237 493
Size: 711 1677 951 2405 2702
Fragments arranged by size:
2702 2405 1677 951 711
BsaBI GATnn'nnATC
Cuts at: 2153 8440 2153
Size: 6287 2159
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3883 4841 5147 5424
Size: 53 83 186 936 2350 958 306 277
Cuts at: 5424 6253 6635 7270 275
Size: 829 382 635 1451
Fragments arranged by size:
2350 1451 958 936 829 635 382 306
277 186 83 53
BsaWI w'CCGG_w
Cuts at: 2089 6879 7277 7551 7698 8109 2089
Size: 4790 398 274 147 411 2426
Fragments arranged by size:
4790 2426 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2511 2541 2867 2897 2992
Size: 30 165 30 1108 30 326 30 95
Cuts at: 2992 3022 3291 3321 5324 5354 1178
Size: 30 269 30 2003 30 4270
Fragments arranged by size:
4270 2003 1108 326 269 165 95 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2264 2579 2777 2888 3869 4025 4367 5348
Size: 1078 315 198 111 981 156 342 981
Cuts at: 5348 5690 1186
Size: 342 3942
Fragments arranged by size:
3942 1078 981 981 342 342 315 198
156 111
BseYI C'CCAG_C
Cuts at: 2649 2855 3903 4262 4363 4641 5113 5233 7649
Size: 206 1048 359 101 278 472 120 2416
Cuts at: 7649 8145 2649
Size: 496 2950
Fragments arranged by size:
2950 2416 1048 496 472 359 278 206
120 101
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3242 5291 5405 1163
Size: 573 1506 2049 114 4204
Fragments arranged by size:
4204 2049 1506 573 114
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4623 5896 6657 7685 1540
Size: 275 186 2622 1273 761 1028 2301
Fragments arranged by size:
2622 2301 1273 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2880 2937 3563 3587 3690 3843 3954 5196 5223
Size: 57 626 24 103 153 111 1242 27
Cuts at: 5223 6507 6592 7663 2880
Size: 1284 85 1071 3663
Fragments arranged by size:
3663 1284 1242 1071 626 153 111 103
85 57 27 24
BsmI GAATG_Cn'
Cuts at: 3456 4935 5957 6050 3456
Size: 1479 1022 93 5852
Fragments arranged by size:
5852 1479 1022 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3012 3374 5530 3012
Size: 362 2156 5928
Fragments arranged by size:
5928 2156 362
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2055 2067 2073 2097 2880 2937
Size: 399 259 6 12 6 24 783 57
Cuts at: 2937 3563 3587 3690 3843 3954 4263 4487 4745
Size: 626 24 103 153 111 309 224 258
Cuts at: 4745 4901 5024 5035 5196 5223 5240 5269 5765
Size: 156 123 11 161 27 17 29 496
Cuts at: 5765 6507 6592 7663 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 783 742 626 496 399 309
259 258 224 161 156 153 123 111
103 85 57 29 27 24 24 17
12 11 6 6
BspEI T'CCGG_A
Cuts at: 8109 8109
Size: 8446
BspHI T'CATG_A
Cuts at: 6335 8065 6335
Size: 1730 6716
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3227 5801 844
Size: 2383 2574 3489
Fragments arranged by size:
3489 2574 2383
BsrBI CCG'CTC
Cuts at: 1394 1823 2569 3162 3570 5419 6333 1394
Size: 429 746 593 408 1849 914 3507
Fragments arranged by size:
3507 1849 914 746 593 429 408
BsrDI GCAATG_nn'
Cuts at: 65 1248 4307 4676 6941 7115 65
Size: 1183 3059 369 2265 174 1396
Fragments arranged by size:
3059 2265 1396 1183 369 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2655 2896 5588 5663 7090 7277 1700
Size: 110 845 241 2692 75 1427 187 2869
Fragments arranged by size:
2869 2692 1427 845 241 187 110 75
BsrGI T'GTAC_A
Cuts at: 96 3309 96
Size: 3213 5233
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8446
BssSI C'ACGA_G
Cuts at: 4868 6506 7518 4868
Size: 1638 1012 5796
Fragments arranged by size:
5796 1638 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4561 4930 794
Size: 3767 369 4310
Fragments arranged by size:
4310 3767 369
BstBI TT'CG_AA
Cuts at: 7250 7250
Size: 8446
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8446
BstXI CCAn_nnnn'nTGG
Cuts at: 3025 3109 3916 3025
Size: 84 807 7555
Fragments arranged by size:
7555 807 84
BstYI r'GATC_y
Cuts at: 1972 3922 4118 5278 5445 5776 6143 6528 6545
Size: 1950 196 1160 167 331 367 385 17
Cuts at: 6545 7997 8083 8095 8441 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1950 1452 1160 385 367 346 331
196 167 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5736 5736
Size: 8446
BtgI C'CryG_G
Cuts at: 513 1065 2004 3208 3774 5016 5604 5742 513
Size: 552 939 1204 566 1242 588 138 3217
Fragments arranged by size:
3217 1242 1204 939 588 566 552 138
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2574 487
Size: 683 56 1348 6359
Fragments arranged by size:
6359 1348 683 56
BtsI GCAGTG_nn'
Cuts at: 4080 4201 5962 6756 6776 4080
Size: 121 1761 794 20 5750
Fragments arranged by size:
5750 1761 794 121 20
ClaI AT'CG_AT
Cuts at: 1554 2181 3753 5322 1554
Size: 627 1572 1569 4678
Fragments arranged by size:
4678 1572 1569 627
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8411
DraI TTT'AAA
Cuts at: 5750 5750
Size: 8446
DraIII CAC_nnn'GTG
Cuts at: 4412 5227 4412
Size: 815 7631
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2764 4664 7453 809
Size: 507 1448 1900 2789 1802
Fragments arranged by size:
2789 1900 1802 1448 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2687 3043 5356
Size: 54 1425 50 275 18 857 356 2313
Cuts at: 5356 5893 6782 8411 8
Size: 537 889 1629 43
Fragments arranged by size:
2313 1629 1425 889 857 537 356 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5893 1537
Size: 275 4081 4090
Fragments arranged by size:
4090 4081 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2334 2403 3639 4633 5720 6376 1353
Size: 981 69 1236 994 1087 656 3423
Fragments arranged by size:
3423 1236 1087 994 981 656 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2905 3456 7033 7407 7553 387
Size: 1064 138 1316 551 3577 374 146 1280
Fragments arranged by size:
3577 1316 1280 1064 551 374 146 138
EcoICRI GAG'CTC
Cuts at: 3561 5194 3561
Size: 1633 6813
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2303 2883 3001 3049 4028 4358 4499
Size: 393 671 580 118 48 979 330 141
Cuts at: 4499 4623 4670 4757 4836 4868 5198 5334 5394
Size: 124 47 87 79 32 330 136 60
Cuts at: 5394 5596 5639 6509 7106 7893 8161 1239
Size: 202 43 870 597 787 268 1524
Fragments arranged by size:
1524 979 870 787 671 597 580 393
330 330 268 202 141 136 124 118
87 79 60 48 47 43 32
EcoNI CCTnn'n_nnAGG
Cuts at: 3170 3657 5136 5657 3170
Size: 487 1479 521 5959
Fragments arranged by size:
5959 1479 521 487
EcoO109I rG'GnC_Cy
Cuts at: 2278 3085 3198 3780 4042 4150 4483 4484 5021
Size: 807 113 582 262 108 333 1 537
Cuts at: 5021 5031 5032 5732 2278
Size: 10 1 700 4992
Fragments arranged by size:
4992 807 700 582 537 333 262 113
108 10 1 1
EcoRI G'AATT_C
Cuts at: 2890 5755 6133 2890
Size: 2865 378 5203
Fragments arranged by size:
5203 2865 378
EcoRV GAT'ATC
Cuts at: 3748 3748
Size: 8446
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2363 2363
Size: 8446
FspI TGC'GCA
Cuts at: 6952 6952
Size: 8446
HaeII r_GCGC'y
Cuts at: 1678 2364 5150 7593 1678
Size: 686 2786 2443 2531
Fragments arranged by size:
2786 2531 2443 686
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2705 2737 2897 2929 2978
Size: 32 135 32 1133 32 160 32 49
Cuts at: 2978 3010 3707 3735 3739 3767 7087 7119 7161
Size: 32 697 28 4 28 3320 32 42
Cuts at: 7161 7193 1373
Size: 32 2626
Fragments arranged by size:
3320 2626 1133 697 160 135 49 42
32 32 32 32 32 32 32 28
28 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2370 4141 4669 5790 6036 669
Size: 988 281 432 1771 528 1121 246 3079
Fragments arranged by size:
3079 1771 1121 988 528 432 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8446
HpaI GTT'AAC
Cuts at: 6036 6036
Size: 8446
KasI G'GCGC_C
Cuts at: 5146 5146
Size: 8446
KpnI G_GTAC'C
Cuts at: 5771 5771
Size: 8446
MfeI C'AATT_G
Cuts at: 2610 6045 2610
Size: 3435 5011
MluI A'CGCG_T
Cuts at: 8322 8322
Size: 8446
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2253 3606 3866 7560 7744 1305
Size: 948 1353 260 3694 184 2007
Fragments arranged by size:
3694 2007 1353 948 260 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2689 10
Size: 54 1425 343 857 5767
Fragments arranged by size:
5767 1425 857 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3830 6404 6763 6922 518
Size: 648 2664 2574 359 159 2042
Fragments arranged by size:
2664 2574 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2024 2323 5361 5606 5862 6539 7687 7932
Size: 418 299 3038 245 256 677 1148 245
Cuts at: 7932 1606
Size: 2120
Fragments arranged by size:
3038 2120 1148 677 418 299 256 245
245
NaeI GCC'GGC
Cuts at: 5590 5665 5590
Size: 75 8371
NarI GG'CG_CC
Cuts at: 5147 5147
Size: 8446
NcoI C'CATG_G
Cuts at: 513 1065 2004 3774 5016 5742 513
Size: 552 939 1770 1242 726 3217
Fragments arranged by size:
3217 1770 1242 939 726 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8446
NgoMIV G'CCGG_C
Cuts at: 5588 5663 5588
Size: 75 8371
NheI G'CTAG_C
Cuts at: 1052 2303 4229 1052
Size: 1251 1926 5269
Fragments arranged by size:
5269 1926 1251
NotI GC'GGCC_GC
Cuts at: 5893 5893
Size: 8446
NsiI A_TGCA'T
Cuts at: 4678 4678
Size: 8446
NspI r_CATG'y
Cuts at: 1132 1229 3958 5804 7349 1132
Size: 97 2729 1846 1545 2229
Fragments arranged by size:
2729 2229 1846 1545 97
PasI CC'CwG_GG
Cuts at: 1348 4894 1348
Size: 3546 4900
PciI A'CATG_T
Cuts at: 7345 7345
Size: 8446
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2281 4495 4522 4648 4894 5495 1108
Size: 1173 2214 27 126 246 601 4059
Fragments arranged by size:
4059 2214 1173 601 246 126 27
PfoI T'CCnGG_A
Cuts at: 2157 2901 3927 4813 5214 2157
Size: 744 1026 886 401 5389
Fragments arranged by size:
5389 1026 886 744 401
PmeI GTTT'AAAC
Cuts at: 5750 5750
Size: 8446
PmlI CAC'GTG
Cuts at: 2881 2881
Size: 8446
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6515 6547 8028 8060 6515
Size: 32 1481 32 6901
Fragments arranged by size:
6901 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2278 3198 3780 4042 2278
Size: 920 582 262 6682
Fragments arranged by size:
6682 920 582 262
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8446
PsiI TTA'TAA
Cuts at: 6016 6016
Size: 8446
PspOMI G'GGCC_C
Cuts at: 4483 5020 5031 5236 4483
Size: 537 11 205 7693
Fragments arranged by size:
7693 537 205 11
PstI C_TGCA'G
Cuts at: 830 3224 5707 5798 830
Size: 2394 2483 91 3478
Fragments arranged by size:
3478 2483 2394 91
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8446
PvuII CAG'CTG
Cuts at: 2024 2323 2024
Size: 299 8147
RsrII CG'GwC_CG
Cuts at: 2913 2913
Size: 8446
SacI G_AGCT'C
Cuts at: 3563 5196 3563
Size: 1633 6813
SacII CC_GC'GG
Cuts at: 5607 5607
Size: 8446
SalI G'TCGA_C
Cuts at: 1936 4667 5788 1936
Size: 2731 1121 4594
Fragments arranged by size:
4594 2731 1121
SanDI GG'GwC_CC
Cuts at: 3198 4042 3198
Size: 844 7602
SapI GCTCTTCn'nnn_
Cuts at: 3639 3639
Size: 8446
SbfI CC_TGCA'GG
Cuts at: 5798 5798
Size: 8446
ScaI AGT'ACT
Cuts at: 6694 8406 6694
Size: 1712 6734
SexAI A'CCwGG_T
Cuts at: 4408 4408
Size: 8446
SfcI C'TryA_G
Cuts at: 826 1045 2356 2427 3220 3525 5703 5794 6929
Size: 219 1311 71 793 305 2178 91 1135
Cuts at: 6929 7610 7801 826
Size: 681 191 1471
Fragments arranged by size:
2178 1471 1311 1135 793 681 305 219
191 91 71
SfoI GGC'GCC
Cuts at: 5148 5148
Size: 8446
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8446
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8446
SmaI CCC'GGG
Cuts at: 1872 2031 5270 5773 1872
Size: 159 3239 503 4545
Fragments arranged by size:
4545 3239 503 159
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2633 3509 3728 3734 6550 7451
Size: 197 926 690 876 219 6 2816 901
Cuts at: 7451 7713 820
Size: 262 1553
Fragments arranged by size:
2816 1553 926 901 876 690 262 219
197 6
SnaBI TAC'GTA
Cuts at: 493 6237 493
Size: 5744 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8446
SphI G_CATG'C
Cuts at: 1132 5804 1132
Size: 4672 3774
SspI AAT'ATT
Cuts at: 5 52 6370 5
Size: 47 6318 2081
Fragments arranged by size:
6318 2081 47
StuI AGG'CCT
Cuts at: 1517 5131 1517
Size: 3614 4832
StyI C'CwwG_G
Cuts at: 513 1065 2004 3774 5016 5742 5885 513
Size: 552 939 1770 1242 726 143 3074
Fragments arranged by size:
3074 1770 1242 939 726 552 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2156 3787 4355 5523 5561 6473 6490
Size: 1255 576 1631 568 1168 38 912 17
Cuts at: 6490 6643 6828 7273 325
Size: 153 185 445 1498
Fragments arranged by size:
1631 1498 1255 1168 912 576 568 445
185 153 38 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3309 6692 8404
Size: 274 80 33 51 1443 1332 3383 1712
Cuts at: 8404 96
Size: 138
Fragments arranged by size:
3383 1712 1443 1332 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2992 3024 3291 3323 3691 3723 8406 8438 2992
Size: 32 267 32 368 32 4683 32 3000
Fragments arranged by size:
4683 3000 368 267 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8446
XbaI T'CTAG_A
Cuts at: 5281 5782 5281
Size: 501 7945
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4154 4154
Size: 8446
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8446
XmaI C'CCGG_G
Cuts at: 1870 2029 5268 5771 1870
Size: 159 3239 503 4545
Fragments arranged by size:
4545 3239 503 159
XmnI GAAnn'nnTTC
Cuts at: 3534 6575 3534
Size: 3041 5405
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5425 6254 276
Size: 53 83 186 936 3891 829 2468
Fragments arranged by size:
3891 2468 936 829 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BsiWI BstZ17I
FseI FspAI NruI PacI PspXI PsrI SfiI SrfI
SwaI